CN107648170A - A kind of Korean ginseng stem cell extract sunlight screening skin-protecting product and preparation method thereof - Google Patents
A kind of Korean ginseng stem cell extract sunlight screening skin-protecting product and preparation method thereof Download PDFInfo
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- CN107648170A CN107648170A CN201711053472.3A CN201711053472A CN107648170A CN 107648170 A CN107648170 A CN 107648170A CN 201711053472 A CN201711053472 A CN 201711053472A CN 107648170 A CN107648170 A CN 107648170A
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- stem cell
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
Abstract
The invention discloses a kind of Korean ginseng stem cell extract sunlight screening skin-protecting product and preparation method thereof, the anti-aging skin care product is made up of the component of following parts by weight：40~60 parts of 0.8~2 part of Korean ginseng stem cell extract, 1~5 part of caraway seed extract, 0.5~1 part of Megranate Seed P.E, 0.1~2.5 part of Radix seu Caulis Parabarii extract, 2~5 parts of jojoba oil, 1~3 part of NMF, 0.1~0.4 part of sodium versenate, 0.4~1.5 part of thickener and deionized water.Sun-proof effective active composition is used as using Korean ginseng stem cell extract, caraway seed extract, Megranate Seed P.E and Radix seu Caulis Parabarii extract in the Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, do not add physical sunscreen agent and chemical sun, it is natural gentle non-stimulated, there is good sun-proof and after-sun effect.
Description
Technical field
The present invention relates to skin care field, and in particular to a kind of Korean ginseng stem cell extract sunlight screening skin-protecting product and its preparation
Method.
Background technology
Ultraviolet irradiation in sunlight is to cause one of principal element of skin aging, and excessive ultraviolet irradiation can not only
Skin surface is enough acted on, triggers erythema, skin skin corium on earth can also be penetrated, cause skin darkening and pigment deposition, very
To causing cutaneum carcinoma.Ultraviolet irradiation can also trigger free radical and active oxygen, accelerate skin aging.
Sun-proof is the important measures of pre- antiultraviolet do harm to huamn body, and sunlight screening skin-protecting product is sun-proof conventional of people
Personal care product.In sunlight screening skin-protecting product on the market, physical shielding type sun-screening agent, the ultraviolet suction of chemical synthesis can be largely classified into
Receipts type sun-screening agent and natural plant type sun-screening agent.Physical shielding type sun-screening agent is mainly by reflective agent scatters ultraviolet and to skin
Play a protective role, conventional has Nano titanium dioxide and Zinc oxide powder, but easily triggers because its photocatalytic activity is high
Sunscreen component changes, and security has much room for improvement.Chemical absorbing type sun-screening agent by absorbing UVA or UVB, with heat energy or
Harmless visible luminous effect discharges, so as to protect human body skin from the injury of ultraviolet, but their often photostability
It is poor, easy oxidation deterioration and cause allergic.Natural plant type sun-screening agent contains antiultraviolet and scavenging activated oxygen
Effective active composition, ultraviolet can be absorbed or reduce active oxygen radical caused by ultraviolet, prevent or reduce skin after shining
Damage, and there is skin repair after shining, and security is good, is sun-proof at present and after-sun skin care item one
Individual important research focus.
Korean ginseng stem cell is undifferentiated cell in Korean ginseng body, is the source and signals-modulating center to grow,
It is antitan agent and solarization with very strong self-renewing and lasting splitting ability, and powerful anti-oxidant and activity of fighting against senium
Repair afterwards and a new method is provided.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of Korean ginseng stem cell extract sun-proof skin care
Product, there is the effect of sun-proof and after-sun.
It is another object of the present invention to provide a kind of preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of Korean ginseng stem cell extract sunlight screening skin-protecting product, is made up of the component of following parts by weight：Korean ginseng is dry thin
0.8~2 part of born of the same parents' extract, 1~5 part of caraway seed extract, 0.5~1 part of Megranate Seed P.E, Radix seu Caulis Parabarii extract 0.1~2.5
Part, 2~5 parts of jojoba oil, 1~3 part of NMF, 0.1~0.4 part of sodium versenate, 0.4~1.5 part of thickener and
40~60 parts of deionized water.
Above-mentioned Korean ginseng stem cell extract sunlight screening skin-protecting product is with Korean ginseng stem cell extract, caraway seed extract, stone
Pomegranate seed extract and Radix seu Caulis Parabarii extract do not add physical sunscreen agent and chemical sun, day as sun-proof effective active composition
It is so gentle non-stimulated；Korean ginseng stem cell extract has powerful anti-oxidant and activity of fighting against senium, good after-sun work(
Effect.Caraway seed extract and Megranate Seed P.E contain flavones ingredient, contain chlorogenic acid and flavonoids in Radix seu Caulis Parabarii extract
Chemicals, ultraviolet can be absorbed, repair sun-damaged skin, with proportioning of the Korean ginseng stem cell extract Jing Guo science, had excellent
Different sun-proof and after-sun effect, and steady quality, it is gentle non-stimulated.
As the preferred embodiment of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, by following weight
The component of number is made：1~1.5 part of Korean ginseng stem cell extract, 2~4 parts of caraway seed extract, Megranate Seed P.E 0.6
~0.9 part, 0.3~1.6 part of Radix seu Caulis Parabarii extract, 2~5 parts of jojoba oil, 1~3 part of NMF, sodium versenate
40~60 parts of 0.1~0.4 part, 0.4~1.5 part of thickener and deionized water.
As the preferred embodiment of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, by following weight
The component of number is made：1.2 parts of Korean ginseng stem cell extract, 3 parts of caraway seed extract, 0.7 part of Megranate Seed P.E, red Du
Secondary 1.5 parts of extract, 2~5 parts of jojoba oil, 1~3 part of NMF, 0.1~0.4 part of sodium versenate, thickener
0.4~1.5 part and 40~60 parts of deionized water.
As the preferred embodiment of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, the Korean ginseng
Stem cell is Korean ginseng forming layer stem cell.
As the preferred embodiment of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described Koryo
Joining stem cell extract includes the Korean ginseng stem cell media after culture Korean ginseng stem cell and the cracking of Korean ginseng stem cell
Thing.
As the preferred embodiment of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, the NMF
For at least one of glycerine, propane diols and sorbierite.
As the preferred embodiment of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, the thickener
For xanthans.
The present invention also provides a kind of preparation method of any of the above-described Korean ginseng stem cell extract sunlight screening skin-protecting product, including
Following steps：
(1) the Korean ginseng stem cell media after culture Korean ginseng stem cell and the lysate of Korean ginseng stem cell are freezed
Dry, obtain Korean ginseng stem cell extract；
(2) each component raw material is weighed in proportion；
(3) jojoba oil, sodium versenate is taken to be heated to 70~80 DEG C, constant temperature stirs to being completely dissolved to form oil
Phase；
(4) take glycerine, xanthans, propane diols and deionized water to be heated to 70~80 DEG C, constant temperature stirring, be thoroughly mixed to form
Aqueous phase；
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion；
(6) when the emulsion that step (5) obtains is cooled to 40~45 DEG C, Korean ginseng stem cell extract, caraway seeds are added
Extract, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, and it is dry thin to obtain the Korean ginseng
Born of the same parents' extract sunlight screening skin-protecting product.
As the preferred embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention,
The culture medium is before freeze-drying, through ultrafiltration membrance filter, concentrates filtrate, removes phenol red in culture medium, retains in culture medium
Active component.
As the preferred embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention,
The aperture of the milipore filter is 250nm.
Compared with prior art, the invention has the advantages that：
Extracted in the Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention with Korean ginseng stem cell extract, caraway seeds
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract do not add physical sunscreen agent and chemical sunscreen as sun-proof effective active composition
Agent, it is natural gentle non-stimulated.Korean ginseng stem cell is the source and letter to grow as undifferentiated cell in Korean ginseng body
Number regulation and control center, there is very strong self-renewing and lasting splitting ability, and powerful anti-oxidant and activity of fighting against senium, have
Good after-sun effect.Caraway seed extract and Megranate Seed P.E contain flavones ingredient, contain in Radix seu Caulis Parabarii extract
There are chlorogenic acid and flavonoids chemicals, ultraviolet can be absorbed, repair sun-damaged skin, pass through section with Korean ginseng stem cell extract
Proportioning, there is excellent sun-proof and after-sun effect, and product quality is stable, it is gentle non-stimulated.
Embodiment
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment to the present invention
Further illustrate.It will be appreciated by those skilled in the art that specific embodiment described herein is only to explain the present invention, not
For limiting the present invention.
Test method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
The material that uses, reagent etc., unless otherwise specified, are commercially obtained.
Embodiment 1
A kind of embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described in the present embodiment
The preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 250nm ultrafiltration membrance filter, is concentrated
Filtrate, remove phenol red in culture medium, the active component in reservation culture medium；By culture medium and Korean ginseng forming layer stem cell
Lysate is freeze-dried, and obtains Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
1.2 parts of Korean ginseng stem cell extract, 3 parts of caraway seed extract, 0.7 part of Megranate Seed P.E, red bark of eucommia extraction
40 parts of 1.5 parts of thing, 2 parts of jojoba oil, 3 parts of glycerine, 0.1 part of sodium versenate, 0.4 part of xanthans and deionized water.
Caraway seed extract is purchased from Tianfeng Biological Science ＆ Technology Co., Ltd., Xi'an；Megranate Seed P.E is purchased from Xi'an Tianrui biology
Technology Co., Ltd.；Radix seu Caulis Parabarii extract Xi'an Wei Zhen bio tech ltd.
(3) jojoba oil, sodium versenate is taken to be heated to 70 DEG C, constant temperature stirs to being completely dissolved to form oil phase.
(4) take glycerine, xanthans and deionized water to be heated to 70 DEG C, constant temperature stirring, be thoroughly mixed to form aqueous phase.
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion.
(6) when the emulsion that step (5) obtains is cooled to 40 DEG C, Korean ginseng stem cell extract, caraway seeds extraction are added
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, obtain the Korean ginseng stem cell and carry
Take thing sunlight screening skin-protecting product.
Embodiment 2
A kind of embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described in the present embodiment
The preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 250nm ultrafiltration membrance filter, is concentrated
Filtrate, remove phenol red in culture medium, the active component in reservation culture medium；By culture medium and Korean ginseng forming layer stem cell
Lysate is freeze-dried, and obtains Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
2 parts of Korean ginseng stem cell extract, 1 part of caraway seed extract, 0.5 part of Megranate Seed P.E, Radix seu Caulis Parabarii extract
0.1 part, 5 parts of jojoba oil, 2.3 parts of glycerine, 0.4 part of sodium versenate, 0.4 part of xanthans, 0.7 part of propane diols and go
56 parts of ionized water.
Caraway seed extract is purchased from Tianfeng Biological Science ＆ Technology Co., Ltd., Xi'an；Megranate Seed P.E is purchased from Xi'an Tianrui biology
Technology Co., Ltd.；Radix seu Caulis Parabarii extract Xi'an Wei Zhen bio tech ltd.
(3) jojoba oil, sodium versenate is taken to be heated to 80 DEG C, constant temperature stirs to being completely dissolved to form oil phase.
(4) take glycerine, xanthans, propane diols and deionized water to be heated to 80 DEG C, constant temperature stirring, be thoroughly mixed to form water
Phase.
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion.
(6) when the emulsion that step (5) obtains is cooled to 45 DEG C, Korean ginseng stem cell extract, caraway seeds extraction are added
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, obtain the Korean ginseng stem cell and carry
Take thing sunlight screening skin-protecting product.
Embodiment 3
A kind of embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described in the present embodiment
The preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 250nm ultrafiltration membrance filter, is concentrated
Filtrate, remove phenol red in culture medium, the active component in reservation culture medium；By culture medium and Korean ginseng forming layer stem cell
Lysate is freeze-dried, and obtains Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
0.8 part of Korean ginseng stem cell extract, 5 parts of caraway seed extract, 1 part of Megranate Seed P.E, Radix seu Caulis Parabarii extract
0.5 part, 4 parts of jojoba oil, 2 parts of glycerine, 0.3 part of sodium versenate, 0.8 part of xanthans, 0.6 part of propane diols and go from
Sub- 60 parts of water.
Caraway seed extract is purchased from Tianfeng Biological Science ＆ Technology Co., Ltd., Xi'an；Megranate Seed P.E is purchased from Xi'an Tianrui biology
Technology Co., Ltd.；Radix seu Caulis Parabarii extract Xi'an Wei Zhen bio tech ltd.
(3) jojoba oil, sodium versenate is taken to be heated to 75 DEG C, constant temperature stirs to being completely dissolved to form oil phase.
(4) take glycerine, xanthans, propane diols and deionized water to be heated to 75 DEG C, constant temperature stirring, be thoroughly mixed to form water
Phase.
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion.
(6) when the emulsion that step (5) obtains is cooled to 40 DEG C, Korean ginseng stem cell extract, caraway seeds extraction are added
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, obtain the Korean ginseng stem cell and carry
Take thing sunlight screening skin-protecting product.
Embodiment 4
A kind of embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described in the present embodiment
The preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 250nm ultrafiltration membrance filter, is concentrated
Filtrate, remove phenol red in culture medium, the active component in reservation culture medium；By culture medium and Korean ginseng forming layer stem cell
Lysate is freeze-dried, and obtains Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
1.5 parts of Korean ginseng stem cell extract, 2 parts of caraway seed extract, 0.6 part of Megranate Seed P.E, red bark of eucommia extraction
1.6 parts of thing, 3 parts of jojoba oil, 1 part of glycerine, 0.4 part of sodium versenate, 1.2 parts of xanthans, 0.5 part of propane diols and go
54 parts of ionized water.
Caraway seed extract is purchased from Tianfeng Biological Science ＆ Technology Co., Ltd., Xi'an；Megranate Seed P.E is purchased from Xi'an Tianrui biology
Technology Co., Ltd.；Radix seu Caulis Parabarii extract Xi'an Wei Zhen bio tech ltd.
(3) jojoba oil, sodium versenate is taken to be heated to 70 DEG C, constant temperature stirs to being completely dissolved to form oil phase.
(4) take glycerine, xanthans, propane diols and deionized water to be heated to 70 DEG C, constant temperature stirring, be thoroughly mixed to form water
Phase.
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion.
(6) when the emulsion that step (5) obtains is cooled to 45 DEG C, Korean ginseng stem cell extract, caraway seeds extraction are added
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, obtain the Korean ginseng stem cell and carry
Take thing sunlight screening skin-protecting product.
Embodiment 5
A kind of embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described in the present embodiment
The preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 250nm ultrafiltration membrance filter, is concentrated
Filtrate, remove phenol red in culture medium, the active component in reservation culture medium；By culture medium and Korean ginseng forming layer stem cell
Lysate is freeze-dried, and obtains Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
1 part of Korean ginseng stem cell extract, 4 parts of caraway seed extract, 0.9 part of Megranate Seed P.E, Radix seu Caulis Parabarii extract
60 parts of 0.3 part, 5 parts of jojoba oil, 2 parts of sorbierite, 0.2 part of sodium versenate, 0.7 part of xanthans and deionized water.
Caraway seed extract is purchased from Tianfeng Biological Science ＆ Technology Co., Ltd., Xi'an；Megranate Seed P.E is purchased from Xi'an Tianrui biology
Technology Co., Ltd.；Radix seu Caulis Parabarii extract Xi'an Wei Zhen bio tech ltd.
(3) jojoba oil, sodium versenate is taken to be heated to 70~80 DEG C, constant temperature stirs to being completely dissolved to form oil
Phase.
(4) take sweet xanthans, sorbierite and deionized water to be heated to 70~80 DEG C, constant temperature stirring, be thoroughly mixed to form water
Phase.
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion.
(6) when the emulsion that step (5) obtains is cooled to 40~45 DEG C, Korean ginseng stem cell extract, caraway seeds are added
Extract, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, and it is dry thin to obtain the Korean ginseng
Born of the same parents' extract sunlight screening skin-protecting product.
Embodiment 6
A kind of embodiment of the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product of the present invention, described in the present embodiment
The preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 250nm ultrafiltration membrance filter, is concentrated
Filtrate, remove phenol red in culture medium, the active component in reservation culture medium；By culture medium and Korean ginseng forming layer stem cell
Lysate is freeze-dried, and obtains Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
1.3 parts of Korean ginseng stem cell extract, 2.5 parts of caraway seed extract, 0.7 part of Megranate Seed P.E, red bark of eucommia carry
Take 1 part of thing, 2 parts of jojoba oil, 2 parts of glycerine, 0.4 part of sodium versenate, 1.5 parts of xanthans, 0.3 part of propane diols and go
45 parts of ionized water.
Caraway seed extract is purchased from Tianfeng Biological Science ＆ Technology Co., Ltd., Xi'an；Megranate Seed P.E is purchased from Xi'an Tianrui biology
Technology Co., Ltd.；Radix seu Caulis Parabarii extract Xi'an Wei Zhen bio tech ltd.
(3) jojoba oil, sodium versenate is taken to be heated to 70 DEG C, constant temperature stirs to being completely dissolved to form oil phase.
(4) take glycerine, xanthans, propane diols and deionized water to be heated to 70 DEG C, constant temperature stirring, be thoroughly mixed to form water
Phase.
(5) oil phase made from step (3) is instilled while stirring in the aqueous phase that step (4) obtains, after emulsifying is uniform
Obtain emulsion.
(6) when the emulsion that step (5) obtains is cooled to 45 DEG C, Korean ginseng stem cell extract, caraway seeds extraction are added
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, obtain the Korean ginseng stem cell and carry
Take thing sunlight screening skin-protecting product.
Sunlight screening skin-protecting product performance evaluation
First, SPF determines
Determined by SPF-290S SPF analyzer (Optomet rics, the U.S.) anti-prepared by embodiment 1~6
Shine the spf value of skin care item.The preparation method and assay method of sample are with reference to the national evaluation criterion about sun care preparations《Make up
Product hygienic practice》The related request of " sun care preparations SPF (spf value) assay method " is carried out in (2007 editions), and table 1 is
SPF measurement result.
The SPF measurement result of table 1
| Numbering | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 |
| SPF | 12 | 14 | 18 | 17 | 21 | 16 |
As seen from the results in Table 1, the Korean ginseng stem cell extract sunlight screening skin-protecting product that prepared by the present invention has well sun-proof
Effect, by adjusting between Korean ginseng stem cell extract, caraway seed extract, Megranate Seed P.E and Radix seu Caulis Parabarii extract
Compound proportion, sun-proof result can be strengthened.
2nd, ultraviolet absorptivity determines
By the Korean ginseng stem cell extract sunlight screening skin-protecting product obtained by embodiment 1~6 with reference to QB/T2410-1998《It is anti-
Shine the evaluation method ultraviolet absorptivity method of cosmetic UV B areas sun-proof result》It is measured, determines the height obtained by embodiment 1~6
Absorbance of the beautiful ginseng stem cell extract sunlight screening skin-protecting product in wavelength between 280~320nm, calculates UVB according to formula (1) respectively
Absorbance A average value under area's wavelength, as a result as shown in table 2.
UVB：280~320nm
AUVB=(A280+A290+A300+A310+A320)/5 (1)
Absorbance A under table 2UVB areas wavelength
| Absorbance | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 |
| AUVB | 0.914 | 0.998 | 1.515 | 1.569 | 1.853 | 1.322 |
Wherein, AUVBIt is minimum ultraviolet protection to be worth for 0.5~1.0；AUVBIt is medium ultraviolet protection for 1.1~1.5；AUVBFor
1.6~2.0 be that effective UV protects.
The result of table 2 shows that Korean ginseng stem cell extract sunlight screening skin-protecting product prepared by the present invention can effectively absorb UVB areas
Ultraviolet, and embodiment 5 prepare Korean ginseng stem cell extract sunlight screening skin-protecting product there is effective UV protection effect.
3rd, after-sun effect determines
The female volunteers 210 that the age is 20~50 years old are chosen, are randomly divided into 7 groups, every group of each 30 people, volunteer distinguishes
Korean ginseng stem cell extract sunlight screening skin-protecting product prepared by embodiment 1~6 is applied to face, daily in the morning, afternoon and evening it is each once,
Continuous 2 months, control group volunteer did not smeared any skin care item/cosmetics.The sunburn quantity of fore-and-aft observing volunteer is tested, point
Do not counted.
The after-sun measurement result of table 3
Efficiently individual quantity is user's number of sunburn reduction before and after use, is increased using rear sunburn or is not changed into invalid；
Improvement degree=(using the preceding rear sunburn number of sunburn number-use)/uses preceding sunburn number
Table 3 is after-sun measurement result, as shown in Table 3, compared with control group, the Korean ginseng high cell of the invention prepared
UV-induced sunburn phenomenon can be effectively reduced, sun-damaged skin is repaired, there is the effect of after-sun.
4th, human body skin adverse reaction is tested
The female volunteers 180 that the age is 20~50 years old are chosen, are randomly divided into 6 groups, every group of each 30 people.Reference《Make up
Product hygienic practice》The related request of " human skin patch " carries out human body skin adverse reaction experiment in (2007 editions).Point
Korean ginseng stem cell extract sunlight screening skin-protecting product prepared by 0.025g embodiment 1~6 is not added on spot examination device, and with stingless
Spot examination device is attached to the forearm song side of volunteer by sharp adhesive tape, is gently pressed with hand and is allowed to uniformly be attached on skin, despeckle is removed after 24h
Device is tried, observes dermoreaction after 30min, 1h, 2h, 24h respectively, and according to《Skin adverse reaction grade scale table》Record is anti-
Answer result.
The skin adverse reaction grade scale table of table 4
Korean ginseng stem cell extract sunlight screening skin-protecting product skin adverse reaction result prepared by the embodiment 1 of table 5
| Grading system | Number of cases | Ratio |
| 0 | 30 | 100% |
| 1 | 0 | 0 |
| 2 | 0 | 0 |
| 3 | 0 | 0 |
| 4 | 0 | 0 |
Table 5 is Korean ginseng stem cell extract sunlight screening skin-protecting product skin adverse reaction result prepared by embodiment 1.Embodiment
The skin adverse reaction result of beautiful ginseng stem cell sunlight screening skin-protecting product prepared by 2~6 is identical with embodiment.
Result of the test shows that volunteer's result of the test of 1~6 group of the embodiment of the present invention is feminine gender, anti-without bad skin
It should occur, show that Korean ginseng stem cell extract sunlight screening skin-protecting product prepared by the present invention is gently non-stimulated, security is good.
Result above shows there is good sun-proof and solarization in Korean ginseng stem cell extract sunlight screening skin-protecting product of the invention
After repair effect, can absorb ultraviolet, repair sun-damaged skin, and product is gently non-stimulated.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of Korean ginseng stem cell extract sunlight screening skin-protecting product, it is characterised in that be made up of the component of following parts by weight：It is high
Beautiful 0.8~2 part of ginseng stem cell extract, 1~5 part of caraway seed extract, 0.5~1 part of Megranate Seed P.E, Radix seu Caulis Parabarii extract
0.1~2.5 part, 2~5 parts of jojoba oil, 1~3 part of NMF, 0.1~0.4 part of sodium versenate, thickener 0.4~
1.5 parts and 40~60 parts of deionized water.
2. Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 1, it is characterised in that by following parts by weight
Several components are made：1~1.5 part of Korean ginseng stem cell extract, 2~4 parts of caraway seed extract, Megranate Seed P.E 0.6~
0.9 part, 0.3~1.6 part of Radix seu Caulis Parabarii extract, 2~5 parts of jojoba oil, 1~3 part of NMF, sodium versenate 0.1
40~60 parts of~0.4 part, 0.4~1.5 part of thickener and deionized water.
3. Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 1, it is characterised in that by following parts by weight
Several components are made：1.2 parts of Korean ginseng stem cell extract, 3 parts of caraway seed extract, 0.7 part of Megranate Seed P.E, red bark of eucommia
1.5 parts of extract, 2~5 parts of jojoba oil, 1~3 part of NMF, 0.1~0.4 part of sodium versenate, thickener 0.4
~1.5 parts and 40~60 parts of deionized water.
4. Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 1, it is characterised in that the Korean ginseng is done
Cell is Korean ginseng forming layer stem cell.
5. Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 1, it is characterised in that the Korean ginseng is done
Cell extract includes the Korean ginseng stem cell media and the lysate of Korean ginseng stem cell after culture Korean ginseng stem cell.
6. Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 1, it is characterised in that the NMF is
At least one of glycerine, propane diols and sorbierite.
7. Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 1, it is characterised in that the thickener is
Xanthans.
8. the preparation method of the Korean ginseng stem cell extract sunlight screening skin-protecting product according to any one of claim 1~7, it is special
Sign is, comprises the following steps：
(1) the Korean ginseng stem cell media after culture Korean ginseng stem cell and the lysate of Korean ginseng stem cell are freezed dry
It is dry, obtain Korean ginseng stem cell extract；
(2) each component raw material is weighed in proportion；
(3) jojoba oil, sodium versenate is taken to be heated to 70~80 DEG C, constant temperature stirs to being completely dissolved to form oil phase；
(4) humectant, thickener and the deionized water of going bail for are heated to 70~80 DEG C, constant temperature stirring, are thoroughly mixed to form aqueous phase；
(5) instill step (3) while stirring in the aqueous phase that step (4) obtains made from oil phase, obtained after emulsifying is uniform
Emulsion；
(6) when the emulsion that step (5) obtains is cooled to 40~45 DEG C, Korean ginseng stem cell extract, caraway seeds extraction are added
Thing, Megranate Seed P.E and Radix seu Caulis Parabarii extract, stir, and are cooled to room temperature, discharging, obtain the Korean ginseng stem cell and carry
Take thing sunlight screening skin-protecting product.
9. the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 8, it is characterised in that institute
Culture medium is stated before freeze-drying, through ultrafiltration membrance filter, concentrates filtrate, removes phenol red in culture medium, is retained in culture medium
Active component.
10. the preparation method of Korean ginseng stem cell extract sunlight screening skin-protecting product according to claim 9, it is characterised in that
The aperture of the milipore filter is 250nm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110241068A (en) * | 2019-07-25 | 2019-09-17 | 成都清科生物科技有限公司 | A method of it is phenol red in removal cell culture waste liquid |
| CN111035574A (en) * | 2019-11-20 | 2020-04-21 | 湖北研妆实业有限公司 | Amino acid antibacterial spray |
| CN112156063A (en) * | 2020-09-30 | 2021-01-01 | 广州研智化妆品有限公司 | Repair essence containing plant stem cell extract and preparation method thereof |
| US11096885B2 (en) * | 2018-08-29 | 2021-08-24 | Amorepacific Corporation | Composition for enhancing skin elasticity or improving skin wrinkles comprising ginseng cell lysate |
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| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11096885B2 (en) * | 2018-08-29 | 2021-08-24 | Amorepacific Corporation | Composition for enhancing skin elasticity or improving skin wrinkles comprising ginseng cell lysate |
| CN110241068A (en) * | 2019-07-25 | 2019-09-17 | 成都清科生物科技有限公司 | A method of it is phenol red in removal cell culture waste liquid |
| CN110241068B (en) * | 2019-07-25 | 2021-05-04 | 成都清科生物科技有限公司 | Method for removing phenol red in cell culture waste liquid |
| CN111035574A (en) * | 2019-11-20 | 2020-04-21 | 湖北研妆实业有限公司 | Amino acid antibacterial spray |
| CN112156063A (en) * | 2020-09-30 | 2021-01-01 | 广州研智化妆品有限公司 | Repair essence containing plant stem cell extract and preparation method thereof |
| CN112156063B (en) * | 2020-09-30 | 2021-06-15 | 广州研智化妆品有限公司 | Preparation method of repair essence containing plant stem cell extract |
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Application publication date: 20180202 |