CN107628652A - A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity - Google Patents
A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity Download PDFInfo
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- CN107628652A CN107628652A CN201710954382.5A CN201710954382A CN107628652A CN 107628652 A CN107628652 A CN 107628652A CN 201710954382 A CN201710954382 A CN 201710954382A CN 107628652 A CN107628652 A CN 107628652A
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- additive
- influenceed
- heavy metal
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- seawater
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity, additive is made up of following part by weight composition：Glycerine 5.0 7.0%, yeast base 3.0 3.5%, trace metal ion 1.8 2.2%, biotin 0.5 1.0%, accelerator 0.5 1.0%, water, surplus；The trace metal ion is sodium chloride, calcium chloride, one kind of sodium molybdate；It is 8.0 8.2 to adjust pH with phosphate buffer.Using the additive of the present invention, obtained Crassostrea rivularis, software part colours are pure white, black portions or stain are few；And from the angle of mouthfeel, such as it is steamed after, fine and tender taste is smooth.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly to a kind of heavy metal seawater that reduces is to big oyster lysozyme activity
The additive of influence.
Background technology
Crassostrea rivularis (Crassostrea rivularis), also known as big oyster, raw oyster, are the marine products rare greatly of Guangxi Qinzhou four
One of, meat can be eaten raw, can also be processed into dried oyster, oyster sauce.Big oyster oyster meat be high protein, low fat, delicious flavour have it is very high
The aquatic products of nutrition and health care function, oyster meat protein content is nutritious more than 40%, delicious flavour, is known as " marine ox
The title of milk ", while can also be used as medicine.Big oyster meat is rich in taurine, can promote human brain development.Crassostrea rivularis is that the main oyster in China is supported
One of species is grown, belongs to brackish water species, is mainly distributed on the numerous river mouth areas of the South China coastal.Crassostrea rivularis is nutritious, its
Artificial farming is concentrated mainly on Guangdong, Guangxi and the band of Fujian one.Oyster belongs to natural marine product, and the algae in seawater is eaten mainly by filter
Class is earned a living, and cultivation risk is relatively low, production is easily managed, technology is easy to grasp.In recent years, raw oyster cultivation is sent out from beach toward shallow sea
Exhibition, constantly risen from inserting to support toward hanging culture mode development, finished product oyster per mu yield, become the pillar industry of China's Coastal Areas,
Coastal fisherman's increased income has effectively been driven to get rich.
Heavy metal is a kind of typical environmental contaminants, and the source of Heavy Metals In Environment is mainly chemical industry, mining, gold
The industries such as category is smelted and processed, plating, steamer manufacture, and agricultural insecticide, sanitary sewage and rubbish diffusate etc..For life
For object, significant toxicity can be just produced when containing low concentration lead in tissue to organism, organism can produce oxidation should
Swash reaction, influence the 26S Proteasome Structure and Function of cell, it is therefore desirable to which antioxidant system resists issuable cellular damage.
Lysozyme (lysozyme) nickname N-acetylmuramide lycanohydrlase, it is a kind of basic protein water of small-molecular-weight
Enzyme is solved, panimmunity reaction in body is participated in, plays an important roll in body normal defense and nonspecific immunity, be organism
The interior important nospecific immunity factor, frequently as one of horizontal important indicator of evaluation immunity of organism.McHenery etc. is with 30
Kind shellfish is research object, and lysozyme activity can be detected in its glandula digestive, outer embrane, the gill, muscle.Existing research hair
It is existing, can detect the lysozyme of high level in the Crassostrea rivularis gill, and lysozyme be nonspecific immunity important in organism because
One of son, during immunity of organism can not only catalyzing hydrolysis bacteria cell wall and cause bacterolysis dead, also can induce and adjust
Save the synthesis and secretion of other immune factors.It can be seen that including Crassostrea rivularis, the gill of shellfish is preventing pathogenic microorganism from invading
Aspect plays an important role.Crassostrea rivularis is by after the heavy metal stress such as pH value reduction and lead, and gill tissue's lysozyme content is in first
Decline, rear rising, again downward trend.When meeting with stress from outside, metabolism is suppressed Crassostrea rivularis, lysozyme content drop
It is low；Then strengthen because adaptability is metabolized, lysozyme content is gradually increasing, even more than previous level；With the extension of time,
Stress does not release still, and prolonged environmental stimulus exceeds the accommodation of Crassostrea rivularis, causes lysozyme content gradually to drop
It is low, immunity degradation.During breeding production, should try one's best avoids that cultivation water pH value is too low, lead content is too high, once find
It should in time adjust, to ensure that the immunity of Crassostrea rivularis maintains normal level, reduce the generation of disease.
The content of the invention
The invention provides a kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity, it is added to nearly river
In the growing environment of oyster (big oyster), can effectively reduce heavy metal seawater influences on big oyster lysozyme activity.
A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity, the additive is by following part by weight
Composition is made：
The trace metal ion is sodium chloride, calcium chloride, one kind of sodium molybdate；
It is 8.0-8.2 to adjust pH with phosphate buffer；
The accelerator is prepared using following methods：
1) Feedstock treating：By honeysuckle it is clean, it is drying, crush；
2) Steam explosion treatment：The material that step 1) is obtained is put into steam blasting tank, burst pressure 3.0-3.5Mpa, protects
The pressure time maintains 150-200s；
3) distillation is handled：The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil；
4) high-shear homogenizing machine is handled：The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Water of the present invention meets pharmaceutical industry standard.
The usage amount of honeysuckle of the present invention, preferably 1/4-1/ of the volume after crushing equivalent to steam blasting tank volume
3。
It is further preferred that the additive is made up of following part by weight composition：
It is 8.0 to adjust pH with phosphate buffer.
The usage amount of additive of the present invention, preferably 0.03-0.10% (w/w seawater).
Compared with prior art, advantages of the present invention：
1st, now there are some researches show in seawater is acidified, acidifying seawater shows to the inductive effect of Crassostrea rivularis lysozyme activity
Now it is slowly lasting, the lysozyme of high level can be detected in the Crassostrea rivularis gill, and lysozyme is important in organism
One of nonspecific immune factors, during immunity of organism can not only catalyzing hydrolysis bacteria cell wall and cause bacterolysis dead
Die, also can induce the synthesis and secretion for adjusting other immune factors.Crassostrea rivularis is chronically in acidifying seawater, is metabolized and is pressed down
System, lysozyme content reduce, and prolonged environmental stimulus exceeds the accommodation of Crassostrea rivularis, causes lysozyme content gradually to drop
It is low, immunity degradation.And the present invention obtains Crassostrea rivularis, pass through the detection to lysozyme activity, it was demonstrated that alleviate acidifying seawater
Crassostrea rivularis lysozyme activity is influenceed.
2nd, lysozyme content reduces, on the appearance of Crassostrea rivularis be Crassostrea rivularis software part yellowish,
Black portions or stain increase, so as to influence the sale of Crassostrea rivularis；And from the angle of mouthfeel, such as it is steamed after, carnification
Always, smoothness is deteriorated.And use the present invention additive, obtained Crassostrea rivularis, software part colours are pure white, black portions or
Person's stain is few；And from the angle of mouthfeel, such as it is steamed after, fine and tender taste is smooth.
Embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1：
A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity, the additive is by following part by weight
Composition is made：
It is 8.0 to adjust pH with phosphate buffer；
The accelerator is prepared using following methods：
1) Feedstock treating：By honeysuckle it is clean, it is drying, crush；
2) Steam explosion treatment：The material that step 1) is obtained is put into steam blasting tank, and volume is equivalent to steam blasting tank
The 1/4-1/3 of volume, burst pressure 3.0-3.5Mpa, dwell time maintain 150-200s；
3) distillation is handled：The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil；
4) high-shear homogenizing machine is handled：The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Embodiment 2：
A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity, the additive is by following part by weight
Composition is made：
It is 8.2 to adjust pH with phosphate buffer；
The accelerator is prepared using following methods：
1) Feedstock treating：By honeysuckle it is clean, it is drying, crush；
2) Steam explosion treatment：The material that step 1) is obtained is put into steam blasting tank, and volume is equivalent to steam blasting tank
The 1/4-1/3 of volume, burst pressure 3.0-3.5Mpa, dwell time maintain 150-200s；
3) distillation is handled：The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil；
4) high-shear homogenizing machine is handled：The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Embodiment 3：
A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity, the additive is by following part by weight
Composition is made：
It is 8.0 to adjust pH with phosphate buffer；
The accelerator is prepared using following methods：
1) Feedstock treating：By honeysuckle it is clean, it is drying, crush；
2) Steam explosion treatment：The material that step 1) is obtained is put into steam blasting tank, and volume is equivalent to steam blasting tank
The 1/4-1/3 of volume, burst pressure 3.0-3.5Mpa, dwell time maintain 150-200s；
3) distillation is handled：The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil；
4) high-shear homogenizing machine is handled：The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Blank example：
The seawater in big oyster base, without using additive.
Comparative example 1：
Additive is made up of following part by weight composition：
It is 8.0 to adjust pH with phosphate buffer.
Comparative example 2：
Additive is made up of following part by weight composition：
It is 8.0 to adjust pH with phosphate buffer；
The accelerator is prepared using following methods：
1) Feedstock treating：By honeysuckle it is clean, it is drying, crush；
2) water extraction is handled：The material that step 1) is obtained adds 10 times of pure water, and ebuillition of heated extracts 2 times, and 2 is small every time
When, filtering, merge extract solution, be concentrated into 1.05-1.1mg/mL (25 DEG C).
Experimental example：
Crassostrea rivularis used in this experiment is purchased from Qiezhou in April, 2017, is immediately sent to test site, is placed in after washing
7d is temporarily supported in 5mx5mx1.8m indoor cement pit, after measured, locality and experimental field Lead In Sea Water content is low for Crassostrea rivularis
In 0.01mg/L, there was no significant difference.Water law is changed using static state during temporarily supporting, changes water daily 1 time, breeding water is filtering sea
Water, main Water Factors are regulated and controled by sampling site, water temperature (27 ± 1) DEG C, pH value 8.0, salinity 3%, with chrysophyceae, Platymonas helgolandica var
As bait, 24h is continuously oxygenated.Temporarily support after terminating, the death rate<1%, Pass Test requirement, select it is uniform in size [(11.28 ±
1.45) g/ only] Crassostrea rivularis individual be used as biological subject.
Using plumbi nitras as pollutant, plumbi nitras (analysis is pure) is configured to 10mg/mL mother liquor, experiment seawater is prepared
Into 0.05mg/L, 0.5mg/L concentration.
1st, handle
Using acidifying seawater (pH value 7.6) needed for acetic acid allotment experiment before experiment, to prevent deployed seawater from using
During pH value raise, is added dropwise using woven hose uniform speed slow into test chamber acetic acid method stably water pH value.Experiment
Control (pH value 8.0) is used as using normal seawater.
2nd, sample preparation
0d is designated as on the day of on-test, after on-test 5,10,15,20d, take 3 Crassostrea rivularis at random from each bucket,
Every group 9, gill tissue is taken, claims quality, the physiological saline of precooling is added in the ratio of 1: 9 (mass volume ratio), is homogenized on ice, 4
DEG C, 5000r/min centrifugation 10min, take supernatant, be placed in -80 DEG C of ultra low temperature freezers and save backup.
3rd, lysozyme activity determines
Lysozyme activity measure builds up the bacteriolyze enzyme detection kit (A050 of Bioengineering Research Institute's development using Nanjing
Type), operated according to kit operation instruction, each testing sample is tested 3 times.
4th, the usage amount of additive：0.05% (w/w seawater)
5th, result：
Influence lysozyme activity (U/mg) of the different lead concentrations to Crassostrea rivularis lysozyme activity
Conclusion：The sample result of embodiment 3 is better than comparative example.
Claims (4)
1. a kind of reduce the additive that is influenceed on big oyster lysozyme activity of heavy metal seawater, it is characterised in that the additive by with
Lower part by weight composition is made：
The trace metal ion is sodium chloride, calcium chloride, one kind of sodium molybdate；
It is 8.0-8.2 to adjust pH with phosphate buffer；
The accelerator is prepared using following methods：
1) Feedstock treating：By honeysuckle it is clean, it is drying, crush；
2) Steam explosion treatment：The material that step 1) is obtained is put into steam blasting tank, burst pressure 3.0-3.5Mpa, during pressurize
Between maintain 150-200s；
3) distillation is handled：The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steam,
When hot steam is filled in distiller, vapor is introduced into condenser by condenser pipe, then by oil water separator,
Collect essential oil；
4) high-shear homogenizing machine is handled：The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-40m/s,
Time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
2. a kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity according to claim 1, it is special
Sign is that the additive is made up of following part by weight composition：
It is 8.0 to adjust pH with phosphate buffer.
3. a kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity according to claim 1, it is special
Sign is：The usage amount of the honeysuckle, the 1/4-1/3 of volume after crushing equivalent to steam blasting tank volume.
4. a kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity according to claim 1, it is special
Sign is：The water meets pharmaceutical industry standard.
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| CN201710954382.5A CN107628652A (en) | 2017-10-13 | 2017-10-13 | A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107522242A (en) * | 2017-10-13 | 2017-12-29 | 桂林融通科技有限公司 | A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102972660A (en) * | 2012-12-13 | 2013-03-20 | 刘宏生 | Chinese herbal medicine compound bait for improving growing power, immunity and disease resistance of stichopus japonicus |
| CN105296580A (en) * | 2015-11-26 | 2016-02-03 | 桂林华诺威基因药业有限公司 | Fermentation medium of recombinant human epidermal growth factor |
| CN107522242A (en) * | 2017-10-13 | 2017-12-29 | 桂林融通科技有限公司 | A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical |
-
2017
- 2017-10-13 CN CN201710954382.5A patent/CN107628652A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972660A (en) * | 2012-12-13 | 2013-03-20 | 刘宏生 | Chinese herbal medicine compound bait for improving growing power, immunity and disease resistance of stichopus japonicus |
| CN105296580A (en) * | 2015-11-26 | 2016-02-03 | 桂林华诺威基因药业有限公司 | Fermentation medium of recombinant human epidermal growth factor |
| CN107522242A (en) * | 2017-10-13 | 2017-12-29 | 桂林融通科技有限公司 | A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107522242A (en) * | 2017-10-13 | 2017-12-29 | 桂林融通科技有限公司 | A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical |
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