CN107522242A - A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical - Google Patents
A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical Download PDFInfo
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- CN107522242A CN107522242A CN201710952824.2A CN201710952824A CN107522242A CN 107522242 A CN107522242 A CN 107522242A CN 201710952824 A CN201710952824 A CN 201710952824A CN 107522242 A CN107522242 A CN 107522242A
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- additive
- active oxygen
- heavy metal
- damaged
- oxygen radical
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- 241000237502 Ostreidae Species 0.000 title claims abstract description 28
- 239000000654 additive Substances 0.000 title claims abstract description 28
- 230000000996 additive effect Effects 0.000 title claims abstract description 28
- 239000001301 oxygen Substances 0.000 title claims abstract description 28
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 28
- 235000020636 oyster Nutrition 0.000 title claims abstract description 27
- 239000013535 sea water Substances 0.000 title claims abstract description 25
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 239000008363 phosphate buffer Substances 0.000 claims abstract description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 6
- 229910021655 trace metal ion Inorganic materials 0.000 claims abstract description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 3
- 239000001110 calcium chloride Substances 0.000 claims abstract description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 3
- 239000011780 sodium chloride Substances 0.000 claims abstract description 3
- 239000011684 sodium molybdate Substances 0.000 claims abstract description 3
- 235000015393 sodium molybdate Nutrition 0.000 claims abstract description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 12
- 238000005422 blasting Methods 0.000 claims description 10
- 239000000341 volatile oil Substances 0.000 claims description 10
- 241000205585 Aquilegia canadensis Species 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 238000004821 distillation Methods 0.000 claims description 5
- 238000004880 explosion Methods 0.000 claims description 5
- 239000012982 microporous membrane Substances 0.000 claims description 5
- 239000007908 nanoemulsion Substances 0.000 claims description 5
- 239000003921 oil Substances 0.000 claims description 5
- 241001522252 Crassostrea rivularis Species 0.000 abstract description 23
- 239000003086 colorant Substances 0.000 abstract description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 229960002685 biotin Drugs 0.000 abstract 1
- 235000020958 biotin Nutrition 0.000 abstract 1
- 239000011616 biotin Substances 0.000 abstract 1
- 235000011187 glycerol Nutrition 0.000 abstract 1
- 102000016938 Catalase Human genes 0.000 description 17
- 108010053835 Catalase Proteins 0.000 description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 4
- 235000013372 meat Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010025 steaming Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 230000004792 oxidative damage Effects 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 210000002824 peroxisome Anatomy 0.000 description 2
- 241000894007 species Species 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 241000206751 Chrysophyceae Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 108010070551 Meat Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 241000204606 Tetraselmis helgolandica Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000000091 biomarker candidate Substances 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000006342 environmental stress response Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical, additive is made up of following part by weight composition:Glycerine 5.0 7.0%, yeast base 3.0 3.5%, trace metal ion 1.8 2.2%, biotin 0.5 1.0%, accelerator 0.5 1.0%, water, surplus;The trace metal ion is sodium chloride, calcium chloride, one kind of sodium molybdate;It is 8.0 8.2 to adjust pH with phosphate buffer.Using the additive of the present invention, obtained Crassostrea rivularis, software part colours are pure white, black portions or stain are few;And from the angle of mouthfeel, such as it is steamed after, fine and tender taste is smooth.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly to a kind of heavy metal seawater that reduces is to big oyster active oxygen freedom
The additive of base infringement.
Background technology
Crassostrea rivularis (Crassostrea rivularis), also known as big oyster, raw oyster, are the marine products rare greatly of Guangxi Qinzhou four
One of, meat can be eaten raw, can also be processed into dried oyster, oyster sauce.Big oyster oyster meat be high protein, low fat, delicious flavour have it is very high
The aquatic products of nutrition and health care function, oyster meat protein content is nutritious more than 40%, delicious flavour, is known as " marine ox
The title of milk ", while can also be used as medicine.Big oyster meat is rich in taurine, can promote human brain development.Crassostrea rivularis is that the main oyster in China is supported
One of species is grown, belongs to brackish water species, is mainly distributed on the numerous river mouth areas of the South China coastal.Crassostrea rivularis is nutritious, its
Artificial farming is concentrated mainly on Guangdong, Guangxi and the band of Fujian one.Oyster belongs to natural marine product, and the algae in seawater is eaten mainly by filter
Class is earned a living, and cultivation risk is relatively low, production is easily managed, technology is easy to grasp.In recent years, raw oyster cultivation is sent out from beach toward shallow sea
Exhibition, constantly risen from inserting to support toward hanging culture mode development, finished product oyster per mu yield, become the pillar industry of China's Coastal Areas,
Coastal fisherman's increased income has effectively been driven to get rich.
Heavy metal is a kind of typical environmental contaminants, and the source of Heavy Metals In Environment is mainly chemical industry, mining, gold
The industries such as category is smelted and processed, plating, steamer manufacture, and agricultural insecticide, sanitary sewage and rubbish diffusate etc..For life
For object, significant toxicity can be just produced when containing low concentration lead in tissue to organism, organism can produce oxidation should
Swash reaction, influence the 26S Proteasome Structure and Function of cell, it is therefore desirable to which antioxidant system resists issuable cellular damage.
Catalase (CAT), is the enzyme that catalyzing hydrogen peroxide resolves into oxygen and water, is present in the peroxidase precursor of cell
It is interior.Catalase is a kind of enzyme scavenger, also known as catalase, is the desmoenzyme using ferriporphyrin as prothetic group.It can promote H2O2
Molecular oxygen and water are decomposed into, removes internal hydrogen peroxide, so that cell protects against H2O2Murder by poisoning, be biophylaxis body
One of key enzyme of system.Catalase is the marker enzyme of peroxisome, accounts for the 40% of peroxisome enzyme total amount.
Catalase is present in each tissue of all known animals, especially exists in liver with high concentration.Hydrogen peroxide
Enzyme is present in the peroxidating body in red blood cell and some tissues, and its main function is exactly to be catalyzed H2O2It is decomposed into H2O2With O2,
So that H2O2It is unlikely to and O2Reaction generates very harmful-OH under iron chelate effect.Catalase is that body is anti-oxidant
Very crucial a kind of non-specific antioxidase in system, superoxide dismutase (SOD) catalytic activity oxygen freedom can be removed
H caused by base2O2, it is converted into the H nontoxic to body2O、O2, the latter and SOD collective effects can make body from oxygen from
By the attack of base.
Because Crassostrea rivularis lives in estuary region, constantly there is fresh water through this person who lives in exile ocean, along with tide, wave etc.
Influenceing, acute variation often occurs for the living environment of Crassostrea rivularis, and Crassostrea rivularis can be moved unlike other marine organisms,
It can go after profits and advoid disadvantages, and be integrally fixed on rock or other objects and live, thus Crassostrea rivularis itself forms a series of replies
The adaptation mechanism of environment-stress, it is the good material for research environment stress response.Heavy metal is rich in marine organisms body
Collection, which can induce, produces substantial amounts of active oxygen radical, such as H2O2、O2 -, OH etc., enter internal disease to help body to remove
Original, but excessive active oxygen can cause the lipidization degree of organism inner cell film according to active oxygen that is fast, destroying in organism
Balance, and then cause the oxidative damage of organism.Now, organism is the injury for avoiding active oxygen to own cells, can be automatic
Swash intravital oxidative stress system -- Antioxidative Defense System, body Antioxidative Defense System is to ensure Heavy Metal Contamination Stress
Caused by active oxygen balance important regulation system, thus be often used as monitor marine sediment thing biomarker candidate
Thing.Regoli etc. is pointed out, in the oxidative stress path of marine organisms, CAT is to prevent active oxygen species from forming essential point
Son.CAT main function is exactly to be catalyzed H2O2It is decomposed into H2O、O2So that H2O2It is unlikely to and O2Reacted under iron chelate effect
Generate very harmful OH.It is necessary how research reduces the infringement of Crassostrea rivularis active oxygen radical.
The content of the invention
The invention provides a kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical, it is added near
In the growing environment of river oyster (big oyster), it can effectively reduce Crassostrea rivularis and be damaged by active oxygen radical.
A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical, the additive is by following weight ratio
Example composition is made:
The trace metal ion is sodium chloride, calcium chloride, one kind of sodium molybdate;
It is 8.0-8.2 to adjust pH with phosphate buffer;
The accelerator is prepared using following methods:
1) Feedstock treating:By honeysuckle it is clean, it is drying, crush;
2) Steam explosion treatment:The material that step 1) is obtained is put into steam blasting tank, burst pressure 3.0-3.5Mpa, protects
The pressure time maintains 150-200s;
3) distillation is handled:The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil;
4) high-shear homogenizing machine is handled:The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Water of the present invention meets pharmaceutical industry standard.
The usage amount of honeysuckle of the present invention, preferably 1/4-1/ of the volume after crushing equivalent to steam blasting tank volume
3。
It is further preferred that the additive is made up of following part by weight composition:
It is 8.0 to adjust pH with phosphate buffer.
The usage amount of additive of the present invention, preferably 0.03-0.10% (w/w seawater).
Compared with prior art, advantages of the present invention:
1st, now there are some researches show in normal seawater, induction of the heavy metal such as low concentration lead to Crassostrea rivularis CAT activity is imitated
It should show slowly lasting, high-concentration lead shows soon to the inductive effect of Crassostrea rivularis CAT activity, excessive active oxygen meeting
Cause the lipidization degree of organism inner cell film according to active oxygen balance that is fast, destroying in organism, and then cause organism
Oxidative damage.And the present invention obtains Crassostrea rivularis, pass through the detection to CAT activity, it was demonstrated that reduce heavy metal seawater to nearly river
Oyster is influenceed by active oxygen radical infringement.
2nd, active oxygen radical damages, and is the software part colours hair of Crassostrea rivularis on the appearance of Crassostrea rivularis
Yellow, black portions or stain increase, so as to influence the sale of Crassostrea rivularis;And from the angle of mouthfeel, such as it is steamed after, meat
Ageing, smoothness is deteriorated.And the additive of the present invention is used, obtained Crassostrea rivularis, software part colours are pure white, black portions
Or stain is few;And from the angle of mouthfeel, such as it is steamed after, fine and tender taste is smooth.
Embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1:
A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical, the additive is by following weight ratio
Example composition is made:
It is 8.0 to adjust pH with phosphate buffer;
The accelerator is prepared using following methods:
1) Feedstock treating:By honeysuckle it is clean, it is drying, crush;
2) Steam explosion treatment:The material that step 1) is obtained is put into steam blasting tank, and volume is equivalent to steam blasting tank
The 1/4-1/3 of volume, burst pressure 3.0-3.5Mpa, dwell time maintain 150-200s;
3) distillation is handled:The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil;
4) high-shear homogenizing machine is handled:The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Embodiment 2:
A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical, the additive is by following weight ratio
Example composition is made:
It is 8.2 to adjust pH with phosphate buffer;
The accelerator is prepared using following methods:
1) Feedstock treating:By honeysuckle it is clean, it is drying, crush;
2) Steam explosion treatment:The material that step 1) is obtained is put into steam blasting tank, and volume is equivalent to steam blasting tank
The 1/4-1/3 of volume, burst pressure 3.0-3.5Mpa, dwell time maintain 150-200s;
3) distillation is handled:The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil;
4) high-shear homogenizing machine is handled:The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Embodiment 3:
A kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical, the additive is by following weight ratio
Example composition is made:
It is 8.0 to adjust pH with phosphate buffer;
The accelerator is prepared using following methods:
1) Feedstock treating:By honeysuckle it is clean, it is drying, crush;
2) Steam explosion treatment:The material that step 1) is obtained is put into steam blasting tank, and volume is equivalent to steam blasting tank
The 1/4-1/3 of volume, burst pressure 3.0-3.5Mpa, dwell time maintain 150-200s;
3) distillation is handled:The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steaming
Vapour, when hot steam is filled in distiller, vapor is introduced into condenser, then pass through water-oil separating by condenser pipe
Device, collect essential oil;
4) high-shear homogenizing machine is handled:The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-
40m/s, time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
Blank example:
The seawater in big oyster base, without using additive.
Comparative example 1:
Additive is made up of following part by weight composition:
It is 8.0 to adjust pH with phosphate buffer.
Comparative example 2:
Additive is made up of following part by weight composition:
It is 8.0 to adjust pH with phosphate buffer;
The accelerator is prepared using following methods:
1) Feedstock treating:By honeysuckle it is clean, it is drying, crush;
2) water extraction is handled:The material that step 1) is obtained adds 10 times of pure water, and ebuillition of heated extracts 2 times, and 2 is small every time
When, filtering, merge extract solution, be concentrated into 1.05-1.1mg/mL (25 DEG C).
Experimental example:
Crassostrea rivularis used in this experiment is purchased from Qiezhou in April, 2017, is immediately sent to test site, is placed in after washing
7d is temporarily supported in 5mx5mx1.8m indoor cement pit, after measured, locality and experimental field Lead In Sea Water content is low for Crassostrea rivularis
In 0.01mg/L, there was no significant difference.Water law is changed using static state during temporarily supporting, changes water daily 1 time, breeding water is filtering sea
Water, main Water Factors are regulated and controled by sampling site, water temperature (27 ± 1) DEG C, pH value 8.0, salinity 3%, with chrysophyceae, Platymonas helgolandica var
As bait, 24h is continuously oxygenated.Temporarily support after terminating, the death rate<1%, Pass Test requirement, select it is uniform in size [(11.28 ±
1.45) g/ only] Crassostrea rivularis individual be used as biological subject.
Using plumbi nitras as pollutant, plumbi nitras (analysis is pure) is configured to 10mg/mL mother liquor, experiment seawater is prepared
Into 0.05mg/L, 0.5mg/L concentration.
1st, handle
Using acidifying seawater (pH value 7.6) needed for acetic acid allotment experiment before experiment, to prevent deployed seawater from using
During pH value raise, is added dropwise using woven hose uniform speed slow into test chamber acetic acid method stably water pH value.Experiment
Control (pH value 8.0) is used as using normal seawater.
2nd, sample preparation
0d is designated as on the day of on-test, after on-test 5,10,15,20d, take 3 Crassostrea rivularis at random from each bucket,
Every group 9, gill tissue is taken, claims quality, by 1:The physiological saline of the ratio addition precooling of 9 (mass volume ratios), is homogenized, 4 on ice
DEG C, 5000r/min centrifugation 10min, take supernatant, be placed in -80 DEG C of ultra low temperature freezers and save backup.
3rd, CAT determinations of activity
The CAT detection kits (A007-1) of Bioengineering Research Institute's development are built up in CAT determinations of activity using Nanjing, according to
Kit operation instruction is operated, and each testing sample is tested 3 times.
4th, the usage amount of additive:0.05% (w/w seawater)
5th, result:
Different lead concentrations are active (U/mg) to the influence CAT of Crassostrea rivularis CAT activity
Conclusion:The sample result of embodiment 3 is better than comparative example.
Claims (4)
1. a kind of reduce the additive that is damaged to big oyster active oxygen radical of heavy metal seawater, it is characterised in that the additive by
Following part by weight composition is made:
The trace metal ion is sodium chloride, calcium chloride, one kind of sodium molybdate;
It is 8.0-8.2 to adjust pH with phosphate buffer;
The accelerator is prepared using following methods:
1) Feedstock treating:By honeysuckle it is clean, it is drying, crush;
2) Steam explosion treatment:The material that step 1) is obtained is put into steam blasting tank, burst pressure 3.0-3.5Mpa, during pressurize
Between maintain 150-200s;
3) distillation is handled:The material that step 2) is obtained is put into distiller, in distiller bottom, heats burning or is passed through steam,
When hot steam is filled in distiller, vapor is introduced into condenser by condenser pipe, then by oil water separator,
Collect essential oil;
4) high-shear homogenizing machine is handled:The essential oil that step 3) is obtained is handled by high-shear homogenizing machine, linear velocity 30-40m/s,
Time 3-5min, nano-emulsion is made, and is obtained with filtering with microporous membrane is degerming.
2. a kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical according to claim 1, its
It is characterised by, the additive is made up of following part by weight composition:
It is 8.0 to adjust pH with phosphate buffer.
3. a kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical according to claim 1, its
It is characterised by:The usage amount of the honeysuckle, the 1/4-1/3 of volume after crushing equivalent to steam blasting tank volume.
4. a kind of additive for reducing heavy metal seawater and being damaged to big oyster active oxygen radical according to claim 1, its
It is characterised by:The water meets pharmaceutical industry standard.
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Cited By (1)
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CN107628652A (en) * | 2017-10-13 | 2018-01-26 | 桂林融通科技有限公司 | A kind of additive for reducing heavy metal seawater and being influenceed on big oyster lysozyme activity |
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