CN107625796A - A kind of medical composition and its use containing the root of Dahurain angelica - Google Patents
A kind of medical composition and its use containing the root of Dahurain angelica Download PDFInfo
- Publication number
- CN107625796A CN107625796A CN201710957675.9A CN201710957675A CN107625796A CN 107625796 A CN107625796 A CN 107625796A CN 201710957675 A CN201710957675 A CN 201710957675A CN 107625796 A CN107625796 A CN 107625796A
- Authority
- CN
- China
- Prior art keywords
- root
- dahurain angelica
- extract
- hours
- ethanol solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to tcm field, is related to a kind of medical composition and its use containing the root of Dahurain angelica.The invention further relates to the new application of the root of Dahurain angelica.In particular it relates to a kind of pharmaceutical composition, it is selected from any one of following (1) to (8) item comprising effective dose：(1) root of Dahurain angelica, (2) root of Dahurain angelica and borneol, (3) root of Dahurain angelica and Ligusticum wallichii, (4) root of Dahurain angelica, borneol and Ligusticum wallichii, (5) Angelica Dahurica extract, (6) Angelica Dahurica extract and borneol, (7) root of Dahurain angelica Rhizoma Chuanxiong extract, and (8) root of Dahurain angelica Rhizoma Chuanxiong extract and borneol；Alternatively, described pharmaceutical composition is also comprising one or more pharmaceutically acceptable auxiliary materials.The present invention can effectively treat or prevention of depression.
Description
Technical field
The invention belongs to tcm field, is related to a kind of medical composition and its use containing the root of Dahurain angelica.The invention further relates to
The new application of the root of Dahurain angelica.
Background technology
Depression is a kind of disease increasingly to draw attention, and it is clinically so that notable and lasting mental state is low, thinking
It is slow and it is movable be reduced to principal character, and mental state is low unbecoming with its situation, and severe patient may occur in which suicidal thought and row
For.The specific cause of disease, the pathogenesis of depression it is not immediately clear.
Pharmaceutical intervention is to treat the Main Means of depression, and clinically conventional antidepressant mainly has：5-HT rephotographies
Take inhibitor (SSRI), the dual reuptake inhibitors of 5-HT/NE (SNRIs), Iricyclic antidepressants (TCAs), and monoamine
Oxidase inhibitor (MAOIs) etc..However, these medicines have many weak points, such as efficient low, onset time prolongs
Late, more than adverse reaction etc..
The root of Dahurain angelica (Angelica dahurica) is the common medicine of tcm clinical practice, be used as medicine first appeared in《Sheng Nong's herbal classic》, it is classified as
Middle product medicine.Its is warm-natured, fragrance, pungent, slight bitter, return lung, stomach, large intestine channel, has the work(such as expelling cold, eliminating, sensible analgesic, detumescence and apocenosis
Effect.Clinic is mainly used in treating cold headache, nasal obstruction, nasosinusitis, toothache, leukorrhea and sore swell and ache curative etc., is also used for treating psoriasis
Deng disease.
Borneol has the effect of " lead to all keys, dissipate stagnated fire ".Frequently as " efficacy-enhancing ingredient " in many Chinese patent drugs, to increase other medicines
The therapeutic effect of thing, document prompting may promote medicine to pass through blood-brain barrier.
Ligusticum wallichii is the dry rhizome of samphire Ligusticum wallichii, and gas is aromatic, bitterly, property pungent-warm, is attributed to liver, courage, pericardium channel.
There is the effect of blood-activating and qi-promoting, wind-expelling pain-stopping, be usually used in irregular menstruation, closed dysmenorrhea, chest side of body shouting pain, tumbling and swelling, headache, rheumatism
Numbness pain etc..
At present, new antidepressant will be developed by still needing.
The content of the invention
The present inventor passes through in-depth study and performing creative labour, it has surprisingly been found that the root of Dahurain angelica and answering comprising the root of Dahurain angelica
Side being capable of effective antidepressant effect.Thus provide following inventions：
One aspect of the present invention is related to a kind of pharmaceutical composition, and it is selected from following (1) to (8) item comprising effective dose
Any one of：
(1) root of Dahurain angelica,
(2) root of Dahurain angelica and borneol,
(3) root of Dahurain angelica and Ligusticum wallichii,
(4) root of Dahurain angelica, borneol and Ligusticum wallichii,
(5) Angelica Dahurica extract,
(6) Angelica Dahurica extract and borneol,
(7) root of Dahurain angelica Rhizoma Chuanxiong extract, and
(8) root of Dahurain angelica Rhizoma Chuanxiong extract and borneol；
Alternatively, described pharmaceutical composition is also comprising one or more pharmaceutically acceptable auxiliary materials.
In the present invention, any one of above-mentioned (1) to (8) item is the active ingredient or active principle of pharmaceutical composition.
In some embodiments of the present invention, the mass ratio of the root of Dahurain angelica and borneol is 180：(0.1-5), preferably 180：
(0.5-1.5), more preferably 180：(0.6-1.2), particularly preferably 180：1.
In some embodiments of the present invention, the mass ratio of the root of Dahurain angelica and Ligusticum wallichii is 1：(0.1-5), preferably 1：(0.2-
1.5), more preferably 1：(0.4-0.8), particularly preferably 1：0.6.
In some embodiments of the present invention, the borneol is powder type.
In some embodiments of the present invention, the root of Dahurain angelica Rhizoma Chuanxiong extract carries for what the common extraction of root of Dahurain angelica Ligusticum wallichii obtained
Thing is taken, or is Angelica Dahurica extract and the mixture of Rhizoma Chuanxiong extract.
In some embodiments of the present invention, the Angelica Dahurica extract is made as follows：
The root of Dahurain angelica is extracted with ethanol solution, obtains extract solution；
Wherein, the envelope-bulk to weight ratio (L/kg) of ethanol solution and the root of Dahurain angelica used is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
In some embodiments of the present invention, it is characterised in that any one in following (1) to (10) item or
It is multinomial：
(1) before extracting, the root of Dahurain angelica is crushed；
(2) before extracting, the root of Dahurain angelica is soaked with ethanol solution；Preferably, soak time be at least 1 hour, at least 2
Hour, at least 6 hours, at least 12 hours or at least 24 hours, such as 12-48 hours or 12-36 hours；
(3) envelope-bulk to weight ratio of ethanol solution used in and the root of Dahurain angelica (L/kg) is 1-15,1-10,1-5,5-15 or 8-12, example
Such as 8,9,10,11 or 12；
(4) volumetric concentration of ethanol solution used in be 50%-95%, 50%-90%, 60%-95%, 60%-90%,
70%-95%, 70%-90%, 80%-95% or 80%-90%, for example, 80%, 81%, 82%, 83%, 84%, 85%,
86%th, 87%, 88%, 89% or 90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or
0.5-2 hours, such as 0.5 hour, 1 hour, 1.5 hours or 2 hours；
(6) Extracting temperature be 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C, such as 21,22 DEG C, 23 DEG C, 24 DEG C, 25
DEG C, 26 DEG C, 27 DEG C, 28 DEG C, 29 DEG C or 30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 2 times or 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
In some embodiments of the present invention, the root of Dahurain angelica Rhizoma Chuanxiong extract is made as follows：
The root of Dahurain angelica and Ligusticum wallichii are extracted with ethanol solution simultaneously or respectively, obtain extract solution；
Wherein, the envelope-bulk to weight ratio (L/kg) of ethanol solution used and the root of Dahurain angelica and/or Ligusticum wallichii is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
In some embodiments of the present invention, it is characterised in that any one in following (1) to (10) item or
It is multinomial：
(1) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are crushed；
(2) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are soaked with ethanol solution；Preferably, soak time is at least 1
Hour, at least 2 hours, at least 6 hours, at least 12 hours or at least 24 hours, such as 12-48 hours or 12-36 hours；
(3) envelope-bulk to weight ratio (L/kg) of ethanol solution used in and the root of Dahurain angelica and/or Ligusticum wallichii is 1-15,1-10,1-5,5-15
Or 8-12, such as 8,9,10,11 or 12；
(4) volumetric concentration of ethanol solution used in be 50%-95%, 50%-90%, 60%-95%, 60%-90%,
70%-95%, 70%-90%, 80%-95% or 80%-90%, for example, 80%, 81%, 82%, 83%, 84%, 85%,
86%th, 87%, 88%, 89% or 90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or
0.5-2 hours, such as 0.5 hour, 1 hour, 1.5 hours or 2 hours；
(6) Extracting temperature be 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C, such as 21,22 DEG C, 23 DEG C, 24 DEG C, 25
DEG C, 26 DEG C, 27 DEG C, 28 DEG C, 29 DEG C or 30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 2 times or 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
According to the pharmaceutical composition described in any one of the present invention, it is also comprising one or more pharmaceutically acceptable auxiliary
Material, such as carrier or excipient；Specifically, its be injection, oral liquid, capsule, tablet, granule, pill or extract again
Mix formulation.
Conventional processing methods can be used, parenteral solution, oral liquid, capsule, tablet, the particle of above-mentioned traditional Chinese medicine monomer composition is made
Agent, pill, extract such as remix at the formulation.
Term " composition " means to include the product of each specified composition comprising specified amount, and directly or indirectly from specified
Any product caused by the combination of each specified composition of amount.
Usual pharmaceutical composition of the present invention contains 0.1-90 weight % active ingredient.Pharmaceutical composition can be according to ability
It is prepared by method known to domain.When for this purpose, if it is desired, can be by active ingredient and one or more solids or liquid medicine
Excipient and/or assistant agent combine, and being made can be as the appropriate administration form or dosage form of people.
The pharmaceutical composition of the present invention can be administered in a unit, and method of administration can be enteron aisle or non-bowel, such as mouth
Clothes, muscle, subcutaneous, nasal cavity, oral mucosa, skin, peritonaeum or rectum etc..Form of administration such as tablet, capsule, dripping pill, aerosol
Agent, pill, pulvis, solution, supensoid agent, emulsion, granule, liposome, transdermal agent, buccal tablet, suppository, freeze drying powder injection
Deng.Can be ordinary preparation, sustained release preparation, controlled release preparation and various particulate delivery systems.In order to which unit dosage forms for administration is made
Tablet, various carriers well known in the art can be widely used.Example on carrier is, such as diluent and absorbent, such as
Starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, urea, calcium carbonate, white bole, microcrystalline cellulose
Element, alumina silicate etc.；Wetting agent and adhesive, such as water, glycerine, polyethylene glycol, ethanol, propyl alcohol, starch slurry, dextrin, syrup, honeybee
Honey, glucose solution, mucialga of arabic gummy, gelatine size, sodium carboxymethylcellulose, lac, methylcellulose, potassium phosphate, polyethylene
Pyrrolidones etc.；Disintegrant, such as dry starch, alginate, agar powder, laminaran, sodium acid carbonate and citric acid, carbonic acid
Calcium, polyoxyethylene, sorbitan fatty acid ester, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.；Disintegration suppresses
Agent, such as sucrose, glyceryl tristearate, cocoa butter, hydrogenated oil and fat etc.；Sorbefacient, such as quaternary ammonium salt, dodecyl sulphate
Sodium etc.；Lubricant, such as talcum powder, silica, cornstarch, stearate, boric acid, atoleine, polyethylene glycol etc..Also
Tablet can be further made to coating tablet, such as sugar coated tablet, thin membrane coated tablet, enteric coated tablets, or double-layer tablets and multilayer
Piece.In order to which administration unit is made into pill, various carriers well known in the art can be widely used.Example on carrier is,
Such as diluent and absorbent, as glucose, lactose, starch, cocoa butter, hydrogenated vegetable oil, polyvinylpyrrolidone,
Gelucire, kaolin, talcum powder etc.；Adhesive such as Arabic gum, bassora gum, gelatin, ethanol, honey, liquid sugar, rice paste or face
Paste etc.；Disintegrant, such as agar powder, dry starch, alginate, dodecyl sodium sulfate, methylcellulose, ethyl cellulose
Deng.In order to which administration unit is made into suppository, various carriers well known in the art can be widely used.Example on carrier is,
Such as the ester of polyethylene glycol, lecithin, cocoa butter, higher alcohol, higher alcohol, gelatin, semi-synthetic glyceride etc..In order to which prescription will be given
Capsule is made in member, active ingredient is mixed with above-mentioned various carriers, and thus obtained mixture is placed in hard obviously glue
In capsule or soft capsule.Also microcapsules can be made in active ingredient, be suspended in aqueous medium and forms supensoid agent, can also load ebonite
In capsule or it is made injection application.In order to which administration unit is made into injection preparation, as solution, emulsion, freeze drying powder injection and
Supensoid agent, all diluents commonly used in the art can be used, for example, water, ethanol, polyethylene glycol, 1,3-PD, ethyoxyl
The isooctadecanol of change, polyoxygenated isooctadecanol, Polyoxyethylene Sorbitol Fatty Acid Esters etc..In addition, in order to prepare isotonic injection
Liquid, appropriate sodium chloride, glucose or glycerine can be added into injection preparation, further, it is also possible to add conventional hydrotropy
Agent, buffer, pH adjusting agent etc..
In addition, if desired, can also be added into pharmaceutical preparation colouring agent, preservative, spices, flavouring, sweetener or
Other materials.
The dosage of the pharmaceutical composition of the present invention depends on many factors, such as to be prevented or treated the property of disease
Sex, age, body weight and the individual reaction of matter and the order of severity, patient or animal, method of administration and administration number of times etc..Above-mentioned dose
Amount with ingle dose form or can be divided into several, such as two, three or four dosage forms for administration.Dosage level must be according to specific
Method of administration, the patient's condition of the order of severity for treating the patient's condition and patient to be treated and medical history etc. are selected.But ability
The way in domain is that dosage is since less than the level for obtaining required therapeutic effect and requiring, gradual incremental dose, until
Obtain required effect.
Another aspect of the present invention is related to is preparing treatment and/or prevention suppression selected from any one of following (1) to (8)
Purposes in the medicine of strongly fragrant disease：
(1) root of Dahurain angelica,
(2) root of Dahurain angelica and borneol,
(3) root of Dahurain angelica and Ligusticum wallichii,
(4) root of Dahurain angelica, borneol and Ligusticum wallichii,
(5) Angelica Dahurica extract,
(6) Angelica Dahurica extract and borneol,
(7) root of Dahurain angelica Rhizoma Chuanxiong extract, and
(8) root of Dahurain angelica Rhizoma Chuanxiong extract and borneol.
In some embodiments of the present invention, the mass ratio of the root of Dahurain angelica and borneol is 180：(0.1-5), preferably 180：
(0.5-1.5), more preferably 180：(0.6-1.2), particularly preferably 180：1.
In some embodiments of the present invention, the mass ratio of the root of Dahurain angelica and Ligusticum wallichii is 1：(0.1-5), preferably 1：(0.2-
1.5), more preferably 1：(0.4-0.8), particularly preferably 1：0.6.
In some embodiments of the present invention, the borneol is powder type.
In some embodiments of the present invention, the root of Dahurain angelica Rhizoma Chuanxiong extract carries for what the common extraction of root of Dahurain angelica Ligusticum wallichii obtained
Thing is taken, or is Angelica Dahurica extract and the mixture of Rhizoma Chuanxiong extract.
In some embodiments of the present invention, the Angelica Dahurica extract is made as follows：
The root of Dahurain angelica is extracted with ethanol solution, obtains extract solution；
Wherein, the envelope-bulk to weight ratio (L/kg) of ethanol solution and the root of Dahurain angelica used is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
In some embodiments of the present invention, it is characterised in that any one in following (1) to (10) item or
It is multinomial：
(1) before extracting, the root of Dahurain angelica is crushed；
(2) before extracting, the root of Dahurain angelica is soaked with ethanol solution；Preferably, soak time be at least 1 hour, at least 2
Hour, at least 6 hours, at least 12 hours or at least 24 hours, such as 12-48 hours or 12-36 hours；
(3) envelope-bulk to weight ratio of ethanol solution used in and the root of Dahurain angelica (L/kg) is 1-15,1-10,1-5,5-15 or 8-12, example
Such as 8,9,10,11 or 12；
(4) volumetric concentration of ethanol solution used in be 50%-95%, 50%-90%, 60%-95%, 60%-90%,
70%-95%, 70%-90%, 80%-95% or 80%-90%, for example, 80%, 81%, 82%, 83%, 84%, 85%,
86%th, 87%, 88%, 89% or 90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or
0.5-2 hours, such as 0.5 hour, 1 hour, 1.5 hours or 2 hours；
(6) Extracting temperature be 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C, such as 21,22 DEG C, 23 DEG C, 24 DEG C, 25
DEG C, 26 DEG C, 27 DEG C, 28 DEG C, 29 DEG C or 30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 2 times or 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
In some embodiments of the present invention, the root of Dahurain angelica Rhizoma Chuanxiong extract is made as follows：
The root of Dahurain angelica and Ligusticum wallichii are extracted with ethanol solution simultaneously or respectively, obtain extract solution；
Wherein, the envelope-bulk to weight ratio (L/kg) of ethanol solution used and the root of Dahurain angelica and/or Ligusticum wallichii is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
In some embodiments of the present invention, it is characterised in that any one in following (1) to (10) item or
It is multinomial：
(1) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are crushed；
(2) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are soaked with ethanol solution；Preferably, soak time is at least 1
Hour, at least 2 hours, at least 6 hours, at least 12 hours or at least 24 hours, such as 12-48 hours or 12-36 hours；
(3) envelope-bulk to weight ratio (L/kg) of ethanol solution used in and the root of Dahurain angelica and/or Ligusticum wallichii is 1-15,1-10,1-5,5-15
Or 8-12, such as 8,9,10,11 or 12；
(4) volumetric concentration of ethanol solution used in be 50%-95%, 50%-90%, 60%-95%, 60%-90%,
70%-95%, 70%-90%, 80%-95% or 80%-90%, for example, 80%, 81%, 82%, 83%, 84%, 85%,
86%th, 87%, 88%, 89% or 90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or
0.5-2 hours, such as 0.5 hour, 1 hour, 1.5 hours or 2 hours；
(6) Extracting temperature be 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C, such as 21,22 DEG C, 23 DEG C, 24 DEG C, 25
DEG C, 26 DEG C, 27 DEG C, 28 DEG C, 29 DEG C or 30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 2 times or 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
Another aspect of the invention be related to it is a kind of treat and/or prevention of depression method, including give demand by
The step of examination person is with pharmaceutical composition any one of the present invention of effective dose.
It is to be understood that total consumption per day of the pharmaceutical composition of the present invention must be by attending physician in reliable medical judgment model
Maked decision in enclosing.For any specific patient, specific treatment effective dose level must be described depending on many factors
Factor includes treated obstacle and the order of severity of the obstacle；Used concrete composition；Age of patient, body weight, one
As health status, sex and diet；Administration time, method of administration and excretion rate；Treat the duration；With being applied in combination or simultaneously
The medicine used；And similar factor known to medical field.For example, the way of this area is, the dosage of administration from less than
The level required to required therapeutic effect starts, gradual incremental dose, until obtaining required effect.It is, in general, that this hair
Bright pharmaceutical composition is calculated as active ingredients can be between 0.001-1000mg/ for the dosage of mammal particularly people
Kg body weight/days, such as between 0.01-100mg/kg body weight/days, such as between 0.01-10mg/kg body weight/days.
It can effectively prevent and/or treat various diseases or disease of the present invention according to the pharmaceutical composition of the present invention
Disease.
In the present invention, if not otherwise specified, the root of Dahurain angelica is angelica root.In one embodiment of the invention
In, the root of Dahurain angelica is angelica dahurica decoction pieces.Under normal circumstances, the root of Dahurain angelica is used as medicine for root.
In the present invention, if not otherwise specified, the Ligusticum wallichii is Ligusticum chuanxiong Hort.In one embodiment of the invention
In, the Ligusticum wallichii is ligusticum wallichii decoction piece.
Ethanol solution of the present invention, if not otherwise specified, its concentration are concentration of volume percent.The ethanol
If not otherwise specified, ethanol solution is referred to.
Term " effective dose ", which refers to realize in subject, treats, prevents, mitigates and/or alleviates disease of the present invention
Or the dosage of illness.
Term " subject " can refer to patient or it is other receive pharmaceutical composition of the present invention with treat, prevent, mitigate and/
Or alleviate the animal of disease or illness of the present invention, particularly mammal, such as people, dog, monkey, ox, horse etc..
Term " disease and/or illness " refers to a kind of condition of the subject, the condition and institute of the present invention
State disease and/or illness is relevant.
The beneficial effect of invention
The root of Dahurain angelica and its there is effective antidepressant effect with the compound of borneol and/or Ligusticum wallichii, its composition is few, strong drug action, system
It is standby simple, and without obvious toxic side effect.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.It is unreceipted specific in embodiment
Condition person, the condition suggested according to normal condition or manufacturer are carried out.Agents useful for same or the unreceipted production firm person of instrument, it is
The conventional products of acquisition purchased in market can be passed through.
The root of Dahurain angelica, borneol, Ligusticum wallichii are purchased from Beijing Tongrentang.
Duloxetine hydrochloride is purchased from Shanghai all ages pharmaceutical Co. Ltd.
Preparation example 1：The preparation of Angelica Dahurica extract (medicinal extract)
The root of Dahurain angelica (be used as medicine root) 2kg crushing, volumetric concentration be 85% ethanol solution (1：10, w:V) immersion 24 is small in
When, then ultrasonic extraction 1h, shakes up 1 time, 25 DEG C of Extracting temperature for during which every 15 minutes.Collect No. the 1st extract solution.In the same terms
Under to carried out respectively in the solid dregs of a decoction the 2nd time and the 3rd time extraction.Merge No. 3 extract solutions, abandon the dregs of a decoction.
The extract solution of merging is concentrated under reduced pressure, obtains medicinal extract 150g.
Recovery rate=150g/2000g=7.5%.
Preparation example 2：The preparation of root of Dahurain angelica medicinal extract-borneol composition
Root of Dahurain angelica medicinal extract extracting method is the same as preparation example 1 above.
By borneol and grinding into powder, mixed with preceding same root of Dahurain angelica medicinal extract and be dissolved in 0.5%CMC-Na, borneol：The root of Dahurain angelica presses medicinal material matter
Measure ratio 1：180 into side.
Preparation example 3：The preparation of the root of Dahurain angelica-Rhizoma Chuanxiong extract (medicinal extract)
Root of Dahurain angelica 1kg, Ligusticum wallichii 0.6kg, co-grinding, in 85% ethanol (1：10, w:V) immersion 24 hours in.Then surpass
Sound extracts 1h, shakes up 1 time within during which every 15 minutes, 25 DEG C of Extracting temperature.Collect No. the 1st extract solution.Under the same conditions to solid
The 2nd time and the 3rd time extraction is carried out in the dregs of a decoction respectively.Merge 3 ultrasonic alcohol extracts, abandon the dregs of a decoction.
The extract solution of merging is concentrated under reduced pressure, obtains medicinal extract 111g.
Recovery rate=111g/1600g=6.9%.
Embodiment 1：The antidepressant effect of root of Dahurain angelica simple
1. Tail suspension test evaluates the antidepressant effect of root of Dahurain angelica simple
Experimental animal：ICR mouse, male, SPF levels, 18~20g are limited purchased from Si Beifu (Beijing) Animal Science
Company.
Given the test agent：Angelica Dahurica extract made from preparation example 1, according to recovery rate and the dosage of angelica root, weigh corresponding
The root of Dahurain angelica medicinal extract of dosage, 0.5% sodium carboxymethylcellulose (CMC-Na) is added, suspension is made, for gavage.
Laboratory apparatus：Mouse tail suspension case：25 × 25 × 35cm, roof center rope connect a Small clamp.
Experimental method：Mouse buys rear adaptability raising 1w, is randomly divided into 5 groups by body weight equilibrium, every group 10, is respectively
Solvent control group (vehicle, 0.5%CMC-Na), Duloxetine group (0.02g/kg), root of Dahurain angelica various dose (based on medicinal material,
1g, 3g, 9g/kg) group, the laggard row progress tail-suspention tests of single oral gavage (p.o., 10ml/kg) administration 60min.Adhesive plaster is bonded at small
At the 1cm of rat-tail end, adhesive plaster is clamped with clip, mouse is observed 6min, note from outstanding boot bottom surface about 5cm in position, head is hung by the feet
The 4min accumulative dead time after record.It is that animal stops struggling to judge motionless standard, and body is perpendicular to hang state by the feet, it is static not
It is dynamic.
Experimental result：It is shown in Table 1.
Influence of the root of Dahurain angelica of table 1 to the mouse tail suspension dead time
Note：N=10, compared with solvent control group,*P<0.05,**P<0.01,***P<0.001.
Tail suspension test result shows that the root of Dahurain angelica (9g/kg, p.o.) can shorten the mouse tail suspension dead time, with solvent pair
There is conspicuousness (P according to a group comparing difference<0.01).Single, which gives positive drug Duloxetine (0.02g/kg, p.o.), can shorten outstanding tail
Dead time, there is conspicuousness (P with control group comparing difference<0.001).The above results prompting root of Dahurain angelica 9g/kg has on this model
There is antidepressant effect.
2. mouse swimming test evaluates the antidepressant effect of the root of Dahurain angelica
Experimental animal：ICR mouse, male, SPF levels, 18~20g are limited purchased from Si Beifu (Beijing) Animal Science
Company.
Given the test agent：Angelica Dahurica extract made from preparation example 1, according to recovery rate and the dosage of angelica root, weigh corresponding
The root of Dahurain angelica medicinal extract of dosage, 0.5% sodium carboxymethylcellulose (CMC-Na) is added, suspension is made, for gavage.
Laboratory apparatus：Mouse swimming device：Circular glass container, high 25cm, diameter 10cm；
Experimental method：Mouse buys rear adaptability raising 1w, is randomly divided into 5 groups by body weight equilibrium, every group 10, is respectively
Solvent control group (vehicle, 0.5%CMC-Na), Duloxetine group (0.04g/kg), root of Dahurain angelica various dose (based on medicinal material, 1,
3rd, 9g/kg) group, the laggard row progress forced swim tests of single oral gavage (p.o., 10ml/kg) administration 60min.Mouse is put into height
In 25cm, diameter 10cm, the depth of water 19cm circular glass container, 25 DEG C of water temperature, 6min is observed, 4min's is accumulative motionless after record
Time.It is that animal stops struggling in water to judge motionless standard, and in floating state, only tiny limb motion is with holding head
Keep afloat in portion.
Experimental result：It is shown in Table 2.
Influence of the root of Dahurain angelica of table 2 to the mouse forced swimming test dead time
Note：N=10, compared with control group*P<0.05,**P<0.01,***P<0.0001。
Mouse forced swimming test result shows that the root of Dahurain angelica (9g/kg, p.o.) can shorten mouse non-swimming time, and molten
Agent control group comparing difference has conspicuousness (P<0.05).Single oral gavage, which gives positive drug Duloxetine 0.04g/kg, can shorten mouse
Non-swimming time, there is conspicuousness (P with control group comparing difference<0.001).The above results prompt root of Dahurain angelica 9g/kg in this model
It is upper that there is antidepressant effect.
Embodiment 2：The antidepressant effect of the root of Dahurain angelica-borneol compatibility
1. Tail suspension test evaluates the antidepressant effect of the root of Dahurain angelica-Borneolum Syntheticum extract
Experimental animal：ICR mouse, male, SPF levels, 18~20g are limited purchased from Si Beifu (Beijing) Animal Science
Company.
Given the test agent：Root of Dahurain angelica medicinal extract-borneol composition made from preparation example 2.
Laboratory apparatus：Mouse tail suspension case：25 × 25 × 35cm, roof center rope connect a Small clamp.
Experimental method：Mouse buys rear adaptability raising 1w, is randomly divided into 4 groups by body weight equilibrium, every group 10, is respectively
Solvent control group (vehicle, 0.5%CMC-Na), Duloxetine group (0.02g/kg), root of Dahurain angelica borneol compatibility various dose (are pressed
Medicinal material meter, the root of Dahurain angelica+borneol, 3g/kg+0.017g/kg, 9g/kg+0.05g/kg) group, single oral gavage (p.o., 10ml/kg) administration
The laggard rows of 60min carry out tail-suspention test.Adhesive plaster is bonded at mouse tail end 1cm, adhesive plaster is clamped with clip, it is in hang body by the feet to make mouse
6min is observed from outstanding boot bottom surface about 5cm in position, head, the 4min accumulative dead time after record.Judge that motionless standard is
Thing stops struggling, and body is perpendicular to hang state, transfixion by the feet.
Experimental result：It is shown in Table 3.
Influence of 3 roots of Dahurain angelica of the table-Borneolum Syntheticum extract to the mouse tail suspension dead time
Note：N=10, compared with solvent control group,*P<0.05,**P<0.01,***P<0.001.
Tail suspension test result shows, root of Dahurain angelica borneol compatibility (3g+0.017g/kg, 9g/kg+0.05g/kg) dosage group
The mouse tail suspension dead time can be shortened, have conspicuousness (P respectively with solvent control group comparing difference<0.01, P<0.001).Single
The outstanding tail dead time can be shortened by giving positive drug Duloxetine (0.02g/kg, p.o.), have conspicuousness with control group comparing difference
(P<0.001).The above results prompting root of Dahurain angelica borneol compatibility has antidepressant effect.
2. mouse swimming test evaluates the antidepressant effect of the root of Dahurain angelica-Borneolum Syntheticum extract
Experimental animal：ICR mouse, male, SPF levels, 18~20g are limited purchased from Si Beifu (Beijing) Animal Science
Company.
Given the test agent：Root of Dahurain angelica medicinal extract-borneol composition made from preparation example 2.
Laboratory apparatus：Mouse swimming device：Circular glass container, high 25cm, diameter 10cm；
Experimental method：Mouse buys rear adaptability raising 1w, is randomly divided into 4 groups by body weight equilibrium, every group 10, is respectively
Solvent control group (vehicle, 0.5%CMC-Na), Duloxetine group (0.04g/kg), root of Dahurain angelica borneol compatibility various dose (are pressed
Medicinal material meter, the root of Dahurain angelica+borneol, 3g+0.017g/kg, 9g/kg+0.05g/kg) group, single oral gavage (p.o., 10ml/kg) administration
The laggard rows of 60min carry out forced swim test.Mouse is put into high 25cm, diameter 10cm, the depth of water 19cm circular glass container
In, 25 DEG C of water temperature, 6min is observed, the 4min accumulative dead time after record.It is that animal stops in water to judge motionless standard
Struggle, in floating state, only tiny limb motion is to keep head to keep afloat.
Experimental result：It is shown in Table 4.
Influence of 4 roots of Dahurain angelica of the table-Borneolum Syntheticum extract to mouse non-swimming time
Note：N=10, compared with solvent control group,*P<0.05,***P<0.001。
Tail suspension test result shows, root of Dahurain angelica borneol compatibility (3g/kg+0.017g/kg, 9g/kg+0.05g/kg) dosage
Group can shorten the mouse tail suspension dead time, have conspicuousness (P respectively with solvent control group comparing difference<0.05, P<0.001).It is single
Secondary positive drug Duloxetine (0.04g/kg, p.o.) of giving can shorten the outstanding tail dead time, have significantly with control group comparing difference
Property (P<0.001).The above results prompting root of Dahurain angelica borneol compatibility has antidepressant effect.
In addition, root of Dahurain angelica borneol compound and root of Dahurain angelica list medicine phases ratio, effective dose is down to 3g/kg from 9g/kg, merely from action agent
Seen in amount, compound is better than single drug effect fruit.
Embodiment 3：The antidepressant effect of the root of Dahurain angelica-Ligusticum wallichii compatibility
1. Tail suspension test evaluates the antidepressant effect of the root of Dahurain angelica-Rhizoma Chuanxiong extract
Experimental animal：ICR mouse, male, SPF levels, 18~20g are limited purchased from Si Beifu (Beijing) Animal Science
Company.
Given the test agent：The root of Dahurain angelica-Rhizoma Chuanxiong extract made from preparation example 3, according to recovery rate and the dosage of medicinal material, weigh corresponding
The root of Dahurain angelica-Rhizoma Chuanxiong extractum of dosage, 0.5% sodium carboxymethylcellulose (CMC-Na) is added, suspension is made, for gavage.
Laboratory apparatus：Mouse tail suspension case：25 × 25 × 35cm, roof center rope connect a Small clamp.
Experimental method：Mouse buys rear adaptability raising 1w, is randomly divided into 5 groups by body weight equilibrium, every group 10, is respectively
Solvent control group (vehicle, 0.5%CMC-Na), Duloxetine group (0.02g/kg), root of Dahurain angelica Ligusticum wallichii compatibility various dose (are pressed
Medicinal material meter, the root of Dahurain angelica+Ligusticum wallichii, 2.25g/kg+1.35g/kg, 4.5g/kg+2.7g/kg, 9g/kg+5.4g/kg) group, single oral gavage
The laggard rows of (p.o., 10ml/kg) administration 60min carry out tail-suspention test.Adhesive plaster is bonded at mouse tail end 1cm, clamped with clip
Adhesive plaster, make mouse in hanging position by the feet, 6min is observed from outstanding boot bottom surface about 5cm in head, after record 4min it is accumulative motionless when
Between.It is that animal stops struggling to judge motionless standard, and body is perpendicular to hang state, transfixion by the feet.
Experimental result：It is shown in Table 5.
Influence of 5 roots of Dahurain angelica of the table-Rhizoma Chuanxiong extract to the mouse tail suspension dead time
Note：N=10, compared with solvent control group,*P<0.05,***P<0.001。
Tail suspension test result shows, root of Dahurain angelica Ligusticum wallichii compatibility (4.5g/kg+2.7g/kg, p.o.) can agent shorten mouse and hang
The tail dead time, there is conspicuousness (P with solvent control group comparing difference<0.05).Single gives positive drug Duloxetine (0.02g/
Kg, p.o.) the outstanding tail dead time can be shortened, there is conspicuousness (P with control group comparing difference<0.001).The above results prompt the root of Dahurain angelica
Ligusticum wallichii compatibility has antidepressant effect on this model.
2. mouse swimming test evaluates the antidepressant effect of the root of Dahurain angelica-Rhizoma Chuanxiong extract
Experimental animal：ICR mouse, male, SPF levels, 18~20g are limited purchased from Si Beifu (Beijing) Animal Science
Company.
Given the test agent：The root of Dahurain angelica-Rhizoma Chuanxiong extract made from preparation example 3, according to recovery rate and the dosage of medicinal material, weigh corresponding
The root of Dahurain angelica-Rhizoma Chuanxiong extractum of dosage, 0.5% sodium carboxymethylcellulose (CMC-Na) is added, suspension is made, for gavage.
Laboratory apparatus：Mouse swimming device：Circular glass container, high 25cm, diameter 10cm；
Experimental method：Mouse buys rear adaptability raising 1w, is randomly divided into 5 groups by body weight equilibrium, every group 10, is respectively
Solvent control group (vehicle, 0.5%CMC-Na), Duloxetine group (0.04g/kg), root of Dahurain angelica Ligusticum wallichii compatibility various dose (are pressed
Medicinal material meter, the root of Dahurain angelica+Ligusticum wallichii, 2.25g/kg+1.35g/kg, 4.5g/kg+2.7g/kg, 9g/kg+5.4g/kg) group, single oral gavage
The laggard rows of (p.o., 10ml/kg) administration 60min carry out forced swim test.Mouse is put into high 25cm, diameter 10cm, the depth of water
In 19cm circular glass container, 25 DEG C of water temperature, 6min is observed, the 4min accumulative dead time after record.Judge motionless mark
Standard is that animal stops struggling in water, and in floating state, only tiny limb motion is to keep head to keep afloat.
Experimental result：It is shown in Table 6.
Influence of 6 roots of Dahurain angelica of the table-Rhizoma Chuanxiong extract to the mouse forced swimming test dead time
Note：N=10, compared with control group,*P<0.05,***P<0.001。
Mouse forced swimming test result shows that root of Dahurain angelica Ligusticum wallichii compatibility middle dose group (4.5g/kg+2.7g/kg, p.o.) can
Shorten mouse non-swimming time, have conspicuousness (P with solvent control group comparing difference<0.05).Single oral gavage gives positive drug
Duloxetine 0.04g/kg can shorten mouse non-swimming time, have conspicuousness (P with control group comparing difference<0.001).It is above-mentioned
As a result prompting root of Dahurain angelica Ligusticum wallichii compatibility has antidepressant effect on this model.
Above example 1-3's test result indicates that：Angelica Dahurica extract, the root of Dahurain angelica-borneol compatibility, the root of Dahurain angelica-Ligusticum wallichii compatibility are equal
With effective antidepressant effect.
Although the embodiment of the present invention has obtained detailed description, it will be understood to those of skill in the art that.Root
According to disclosed all teachings, those details can be carried out with various modifications and replacement, these change in the guarantor of the present invention
Within the scope of shield.The four corner of the present invention is provided by appended claims and its any equivalent.
Claims (16)
1. a kind of pharmaceutical composition, it is selected from any one of following (1) to (8) item comprising effective dose：
(1) root of Dahurain angelica,
(2) root of Dahurain angelica and borneol,
(3) root of Dahurain angelica and Ligusticum wallichii,
(4) root of Dahurain angelica, borneol and Ligusticum wallichii,
(5) Angelica Dahurica extract,
(6) Angelica Dahurica extract and borneol,
(7) root of Dahurain angelica Rhizoma Chuanxiong extract, and
(8) root of Dahurain angelica Rhizoma Chuanxiong extract and borneol；
Alternatively, described pharmaceutical composition is also comprising one or more pharmaceutically acceptable auxiliary materials.
2. pharmaceutical composition according to claim 1, wherein in (2), (4), (6) or (8) item, the matter of the root of Dahurain angelica and borneol
Amount is than being 180：(0.1-5), preferably 180：(0.5-1.5), more preferably 180：(0.6-1.2), particularly preferably 180：1.
3. pharmaceutical composition according to claim 1, wherein, in (3), (4), (7) or (8) item, the root of Dahurain angelica and Ligusticum wallichii
Mass ratio is 1：(0.1-5), preferably 1：(0.2-1.5), more preferably 1：(0.4-0.8), particularly preferably 1：0.6.
4. pharmaceutical composition according to claim 1, wherein, the root of Dahurain angelica Rhizoma Chuanxiong extract is that root of Dahurain angelica Ligusticum wallichii extracts jointly
Obtained extract, or be Angelica Dahurica extract and the mixture of Rhizoma Chuanxiong extract.
5. the pharmaceutical composition according to any claim in Claims 1-4, wherein, the Angelica Dahurica extract passes through
Following steps are made：
The root of Dahurain angelica is extracted with ethanol solution, obtains extract solution；
Wherein, the envelope-bulk to weight ratio (L/kg) of ethanol solution and the root of Dahurain angelica used is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
6. pharmaceutical composition according to claim 5, it is characterised in that any one in following (1) to (10) item or
Person is multinomial：
(1) before extracting, the root of Dahurain angelica is crushed；
(2) before extracting, the root of Dahurain angelica is soaked with ethanol solution；Preferably, soak time be at least 1 hour, it is at least 2 small
When, at least 6 hours, at least 12 hours or at least 24 hours；
(3) envelope-bulk to weight ratio of ethanol solution used in and the root of Dahurain angelica (L/kg) is 1-15,1-10,1-5,5-15 or 8-12；
(4) volumetric concentration of ethanol solution used in is 50%-95%, 50%-90%, 60%-95%, 60%-90%, 70%-
95%th, 70%-90%, 80%-95% or 80%-90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or 0.5-
2 hours；
(6) Extracting temperature is 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
7. the pharmaceutical composition according to any claim in Claims 1-4, wherein, the root of Dahurain angelica Rhizoma Chuanxiong extract
It is made as follows：
The root of Dahurain angelica and Ligusticum wallichii are extracted with ethanol solution simultaneously or respectively, obtain extract solution；
Wherein,
The envelope-bulk to weight ratio (L/kg) of ethanol solution used and the root of Dahurain angelica and/or Ligusticum wallichii is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
8. pharmaceutical composition according to claim 7, it is characterised in that any one in following (1) to (10) item or
Person is multinomial：
(1) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are crushed；
(2) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are soaked with ethanol solution；Preferably, soak time be at least 1 hour,
At least 2 hours, at least 6 hours, at least 12 hours or at least 24 hours；
(3) envelope-bulk to weight ratio (L/kg) of ethanol solution used in and the root of Dahurain angelica and/or Ligusticum wallichii is 1-15,1-10,1-5,5-15 or 8-
12；
(4) volumetric concentration of ethanol solution used in is 50%-95%, 50%-90%, 60%-95%, 60%-90%, 70%-
95%th, 70%-90%, 80%-95% or 80%-90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or 0.5-
2 hours；
(6) Extracting temperature is 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
9. the purposes selected from any one of following (1) to (8) in the medicine for the treatment of and/or prevention of depression is prepared：
(1) root of Dahurain angelica,
(2) root of Dahurain angelica and borneol,
(3) root of Dahurain angelica and Ligusticum wallichii,
(4) root of Dahurain angelica, borneol and Ligusticum wallichii,
(5) Angelica Dahurica extract,
(6) Angelica Dahurica extract and borneol,
(7) root of Dahurain angelica Rhizoma Chuanxiong extract, and
(8) root of Dahurain angelica Rhizoma Chuanxiong extract and borneol.
10. purposes according to claim 9, wherein in (2), (4), (6) or (8) item, the mass ratio of the root of Dahurain angelica and borneol
For 180：(0.1-5), preferably 180：(0.5-1.5), more preferably 180：(0.6-1.2), particularly preferably 180：1.
11. purposes according to claim 9, wherein in (3), (4), (7) or (8) item, the mass ratio of the root of Dahurain angelica and Ligusticum wallichii
For 1：(0.1-5), preferably 1：(0.2-1.5), more preferably 1：(0.4-0.8), particularly preferably 1：0.6.
12. purposes according to claim 9, wherein, the root of Dahurain angelica Rhizoma Chuanxiong extract is that extraction obtains root of Dahurain angelica Ligusticum wallichii jointly
Extract, or the mixture for Angelica Dahurica extract and Rhizoma Chuanxiong extract.
13. the purposes according to any claim in claim 9 to 12, wherein, the Angelica Dahurica extract passes through as follows
Step is made：
The root of Dahurain angelica is extracted with ethanol solution, obtains extract solution；
Wherein,
The envelope-bulk to weight ratio (L/kg) of ethanol solution and the root of Dahurain angelica used is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
14. purposes according to claim 13, it is characterised in that any one or more in following (1) to (10) item
：
(1) before extracting, the root of Dahurain angelica is crushed；
(2) before extracting, the root of Dahurain angelica is soaked with ethanol solution；Preferably, soak time be at least 1 hour, it is at least 2 small
When, at least 6 hours, at least 12 hours or at least 24 hours；
(3) envelope-bulk to weight ratio of ethanol solution used in and the root of Dahurain angelica (L/kg) is 1-15,1-10,1-5,5-15 or 8-12；
(4) volumetric concentration of ethanol solution used in is 50%-95%, 50%-90%, 60%-95%, 60%-90%, 70%-
95%th, 70%-90%, 80%-95% or 80%-90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or 0.5-
2 hours；
(6) Extracting temperature is 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
15. the purposes according to any claim in claim 9 to 12, wherein, the root of Dahurain angelica Rhizoma Chuanxiong extract passes through
Following steps are made：
The root of Dahurain angelica and Ligusticum wallichii are extracted with ethanol solution simultaneously or respectively, obtain extract solution；
Wherein, the envelope-bulk to weight ratio (L/kg) of ethanol solution used and the root of Dahurain angelica and/or Ligusticum wallichii is 1-20,
Ethanol solution used is the ethanol solution that volumetric concentration is 50%-99%；
Extracting temperature is 5 DEG C -40 DEG C；
Extraction time is at least 10 minutes.
16. purposes according to claim 15, it is characterised in that any one or more in following (1) to (10) item
：
(1) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are crushed；
(2) before extracting, the root of Dahurain angelica and/or Ligusticum wallichii are soaked with ethanol solution；Preferably, soak time be at least 1 hour,
At least 2 hours, at least 6 hours, at least 12 hours or at least 24 hours；
(3) envelope-bulk to weight ratio (L/kg) of ethanol solution used in and the root of Dahurain angelica and/or Ligusticum wallichii is 1-15,1-10,1-5,5-15 or 8-
12；
(4) volumetric concentration of ethanol solution used in is 50%-95%, 50%-90%, 60%-95%, 60%-90%, 70%-
95%th, 70%-90%, 80%-95% or 80%-90%；
(5) extraction time be at least 20 minutes, at least 0.5 hour, 0.5-24 hours, 0.5-12 hours, 0.5-5 hours or 0.5-
2 hours；
(6) Extracting temperature is 10 DEG C -40 DEG C, 15 DEG C -35 DEG C, 20 DEG C -30 DEG C；
(7) it is stirred or vibrates simultaneously in extraction process；
(8) extract 1 time or multiple, such as 3 times；
(9) extract solution is concentrated and be for example concentrated under reduced pressure, obtain medicinal extract；
(10) described be extracted under ultrasound condition is carried out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710957675.9A CN107625796B (en) | 2017-10-16 | 2017-10-16 | Pharmaceutical composition containing radix angelicae and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710957675.9A CN107625796B (en) | 2017-10-16 | 2017-10-16 | Pharmaceutical composition containing radix angelicae and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107625796A true CN107625796A (en) | 2018-01-26 |
| CN107625796B CN107625796B (en) | 2021-07-06 |
Family
ID=61104374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710957675.9A Active CN107625796B (en) | 2017-10-16 | 2017-10-16 | Pharmaceutical composition containing radix angelicae and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107625796B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116570659A (en) * | 2023-05-25 | 2023-08-11 | 中国人民解放军军事科学院军事医学研究院 | A Chinese medicinal composition for treating depression and insomnia, and its preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634375A (en) * | 2004-11-08 | 2005-07-06 | 贵州吉仁药业有限公司 | Chinese traditional medicine for treating arthralgia due to wind-dampness and preparation method thereof |
| CN1723997A (en) * | 2005-07-08 | 2006-01-25 | 陈陆敢 | Traditional Chinese medicine prepn. for treating mental disease |
| CN1872250A (en) * | 2005-06-01 | 2006-12-06 | 天津天士力制药股份有限公司 | Composition of medication for treating headache |
| CN107048895A (en) * | 2016-10-19 | 2017-08-18 | 天津佰亿达生物科技股份有限公司 | A kind of medicine-filled pillow with health-care efficacy |
-
2017
- 2017-10-16 CN CN201710957675.9A patent/CN107625796B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634375A (en) * | 2004-11-08 | 2005-07-06 | 贵州吉仁药业有限公司 | Chinese traditional medicine for treating arthralgia due to wind-dampness and preparation method thereof |
| CN1872250A (en) * | 2005-06-01 | 2006-12-06 | 天津天士力制药股份有限公司 | Composition of medication for treating headache |
| CN1723997A (en) * | 2005-07-08 | 2006-01-25 | 陈陆敢 | Traditional Chinese medicine prepn. for treating mental disease |
| CN107048895A (en) * | 2016-10-19 | 2017-08-18 | 天津佰亿达生物科技股份有限公司 | A kind of medicine-filled pillow with health-care efficacy |
Non-Patent Citations (7)
| Title |
|---|
| MIN HOU等: "Pharmacodynamic action and mechanism of DuL iang soft capsule, a traditional Chinese medicine capsule, on treating nitroglycerin-induced migraine", 《JOURNAL OF ETHNOPHARMACOLOGY》 * |
| YANJUN CAO等: "Antidepressive-like effect of imperatorin from Angelica dahurica in prenatally stressed offspring rats through 5-hydroxytryptamine system", 《NEUROREPORT》 * |
| 张莹等: "川芎嗪抗抑郁作用研究", 《中药材》 * |
| 梁明金等: "白芷中欧前胡素的提取方法研究", 《中成药》 * |
| 肖衍宇等: "冰片对川芎嗪促吸收作用的研究", 《药学学报》 * |
| 郜文辉: "《有益全身的养生妙招：人体不同部位的保健养生》", 31 July 2017, 中国中医药出版社 * |
| 雷玲等: "三种冰片对大鼠肠道细胞色素P450和糖蛋白P-gp的影响", 《中药药理与临床》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116570659A (en) * | 2023-05-25 | 2023-08-11 | 中国人民解放军军事科学院军事医学研究院 | A Chinese medicinal composition for treating depression and insomnia, and its preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107625796B (en) | 2021-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1840166A (en) | Modern Chinese medicinal oral liquid of 'Wen Dan Tang' and preparation method thereof | |
| CN102319342B (en) | Medicament for relaxing bowel and preparation method thereof | |
| CN104352552A (en) | Food, health care product or medicine composition | |
| CN104208425B (en) | A kind of Chinese medicine preparation for being used to nurse one's health critical hypertension | |
| CN102716135B (en) | Lupenone prevents in preparation or treats the application in the product of diabetes | |
| CN105616679A (en) | Traditional Chinese medicine preparation with effects of nourishing blood and tranquilization and preparation method therefor | |
| CN107625796A (en) | A kind of medical composition and its use containing the root of Dahurain angelica | |
| US20230125425A1 (en) | Traditional chinese medicine extract composition with function of regulating depressive emotion and preparation method and traditional chinese medicine preparation thereof | |
| CN103860565B (en) | Medicinal composition for treating diabetes hepatic fibrosis | |
| CN103768357B (en) | A kind of Traditional Chinese medicine compound composition with treatment recurrent aphtha | |
| CN105169290A (en) | Traditional Chinese medicine composition for treating primary trigeminal neuralgia and application thereof | |
| CN107744571B (en) | Pharmaceutical composition for improving vascular endothelial dysfunction and preparation method and application thereof | |
| CN102861117A (en) | Composition containing Chinese medicine active ingredients and application thereof | |
| CN101433598A (en) | Uses of giant knotweed for preventing and treating insulin resistance and related metabolic diseases thereof | |
| CN106176510A (en) | A kind of oral thing for aesthetic health care and corresponding beautifying health composition | |
| CN102327304B (en) | Drug for preventing and controlling chronic mountain sickness and preparation method thereof | |
| CN108403759A (en) | A kind of health composition and its preparation method and application | |
| CN102309561B (en) | Pharmaceutical composition used for preventing and treating urarthritis and hyperuricemia | |
| CN1903314A (en) | Traditional Chinese medicine composition for treating head-ache, ahypnia, palpitate and feeble, and its prepn. method | |
| CN107753567B (en) | Anti-fatigue pharmaceutical composition and preparation method and application thereof | |
| CN102631497B (en) | Health care composition | |
| CN115624543B (en) | Medicine for treating migraine, pharmaceutical composition, preparation method and pharmaceutical application thereof | |
| CN111643606B (en) | Gel plaster for treating insomnia and preparation method thereof | |
| CN101053595B (en) | Medicinal preparation for treating constipation and its preparing method | |
| CN101579481B (en) | Medicinal composition for treating hypertension |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |