CN107619353A - A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate - Google Patents

A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate Download PDF

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Publication number
CN107619353A
CN107619353A CN201711070937.6A CN201711070937A CN107619353A CN 107619353 A CN107619353 A CN 107619353A CN 201711070937 A CN201711070937 A CN 201711070937A CN 107619353 A CN107619353 A CN 107619353A
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China
Prior art keywords
herba dendrobii
tissue culture
survival rate
matrix
culture seedling
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CN201711070937.6A
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Chinese (zh)
Inventor
张本厚
胡燕花
丁小余
金磊磊
何春阳
徐德林
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INSTITUTE OF DAFENG MARINE INDUSTRY NANJING UNIVERSITY OF TECHNOLOGY
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INSTITUTE OF DAFENG MARINE INDUSTRY NANJING UNIVERSITY OF TECHNOLOGY
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Priority to CN201711070937.6A priority Critical patent/CN107619353A/en
Publication of CN107619353A publication Critical patent/CN107619353A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:Including fat tree skin, wood fragments bits, vermiculite, decay sheep excrement, the volume ratio including fat tree skin, wood fragments bits, vermiculite, decay sheep excrement is:Fat tree skin:Wood fragments are considered to be worth doing:Vermiculite:Decay sheep excrement=8:4:1:0.5.The matrix disclosed in this invention for improving HERBA DENDROBII training tissue culture seedling survival rate, air permeability is good, can prevent rotten of plant offspring, improves HERBA DENDROBII training tissue culture seedling survival rate.

Description

A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate
Technical field
The present invention relates to HERBA DENDROBII technical field of cultivation, more particularly to a kind of HERBA DENDROBII training tissue culture seedling that improves survives The matrix of rate.
Background technology
HERBA DENDROBII category orchid family Dendrobium Sw, it is the rare traditional Chinese medicine of Precious, Rare, Endangered, is listed in one of " nine big celestial grass ". Traditional Chinese Medicine thinks that it has Yin-nourishing and body fluid promoting, strengthens immune, spleen benefiting and stimulating the appetite, protect liver cholagogic, clearing away heat to and alleviating pain, moistens the lung and relieve the cough, enriching yin Heat-clearing, improving eyesight salubrity, reduce blood glucose, profit supports skin, promotes longevity and other effects, to weak and sickly, hypoimmunity, deficiency of Yin body Matter, dry polydipsia, excessive drinking and smoking symptoms such as the weary, post-operative recovery of body of staying up late have the effect of good and remission effect.Modern pharmacology is ground Study carefully and show, HERBA DENDROBII has radioresistance, anti-cancer, antitumor, delaying senility, reduces blood pressure, expands blood vessel, immunoregulatory work With.HERBA DENDROBII is used as medicine with stem, and main chemical compositions have alkaloid, polysaccharide, sesquiterpenoids, luxuriant and rich with fragrance class, bibenzyl etc., and dendrobine is The characteristics index composition of HERBA DENDROBII, and dendrobine has pharmacological activity in terms of blood pressure and blood lipoid is reduced.Moreover, HERBA DENDROBII Flower pattern is unique, beautiful in colour, great ornamental value.Therefore, HERBA DENDROBII is the rare traditional Chinese medicine for having medicinal and ornamental value concurrently. However, due to the seed of HERBA DENDROBII is tiny, without endosperm, fertility is extremely low under field conditions (factors), along with growth is slow naturally Slowly, the mankind's excessive digging for a long time, wild resource is increasingly exhausted, is classified as national endangered second class protection plant by China.
Shortage is alleviated to a certain extent and is asked using the method production HERBA DENDROBII seedling of Plant Tissue Breeding at present Topic, but tissue-cultured seedling needs that after carrying out hardening greenhouse or imitating wild planting could be entered, and hardening quality directly affects the output of seedling, And the selection of matrix and an important factor for using being hardening survival rate, therefore the selection of hardening matrix is most important.It is existing The air permeability of most of matrix is inadequate, easily allows the offspring of plant rotten or slow growing situation occur, causes HERBA DENDROBII Training tissue culture seedling survival rate is relatively low, and cost is cultivated in increase.
The content of the invention
The present invention seeks to:Overcome the shortcomings of the prior art, solve problems of the prior art, there is provided be a kind of The matrix of HERBA DENDROBII training tissue culture seedling survival rate is improved, air permeability is good, can prevent rotten of plant offspring, improves HERBA DENDROBII Training tissue culture seedling survival rate.
The technical scheme is that:
A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:Including fat tree skin, wood fragments bits, Vermiculite, decay sheep excrement, the volume ratio including fat tree skin, wood fragments bits, vermiculite, decay sheep excrement are:Fat tree skin:Wood fragments are considered to be worth doing:Leech Stone:Decay sheep excrement=8:4:1:0.5.
Preferably, the matrix for improving HERBA DENDROBII training tissue culture seedling survival rate also includes carbendazim, the carbendazim The percentage by weight for accounting for matrix is 0.3%-0.5%.
Preferably, the matrix for improving HERBA DENDROBII training tissue culture seedling survival rate also includes organic nutrient, described organic The percentage by weight that nutrient accounts for matrix is 0.02%-0.05%.
Preferably, the organic nutrient includes inositol, vitamin B1, vitamin B6, glycine, nicotinic acid.
Preferably, the inositol, vitamin B1, vitamin B6, glycine, the volume ratio of nicotinic acid are:Inositol:Vitamin B1:Vitamin B6:Glycine:Nicotinic acid=150:1:1:4:1.
Advantages of the present invention:
1st, the matrix disclosed in this invention for improving HERBA DENDROBII training tissue culture seedling survival rate, air permeability is good, can prevent Rotten of plant offspring, improve HERBA DENDROBII training tissue culture seedling survival rate.
2nd, the matrix nutrition value high easily absorption disclosed in this invention for improving HERBA DENDROBII training tissue culture seedling survival rate, Improve emergence rate or the seeding stage shifts to an earlier date, reduce and cultivate cost.
Embodiment
Technical solution of the present invention is described in detail with reference to preferred embodiment.
A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate, it includes fat tree skin, wood fragments bits, vermiculite, decomposed Sheep manure, the volume ratio including fat tree skin, wood fragments bits, vermiculite, decay sheep excrement are:Fat tree skin:Wood fragments are considered to be worth doing:Vermiculite:Decomposed sheep Excrement=8:4:1:0.5.The matrix disclosed in this invention for improving HERBA DENDROBII training tissue culture seedling survival rate, air permeability is good, can be with Rotten of plant offspring is prevented, improves HERBA DENDROBII training tissue culture seedling survival rate.Raising HERBA DENDROBII tissue culture disclosed in this invention The matrix nutrition value of seedling hardening survival rate is high easily to be absorbed, and improves emergence rate or the seeding stage shifts to an earlier date, reduce and cultivate cost.
Preferably, the matrix for improving HERBA DENDROBII training tissue culture seedling survival rate also includes carbendazim, the carbendazim The percentage by weight for accounting for matrix is 0.3%-0.5%.Rotten can be prevented, improves HERBA DENDROBII training tissue culture seedling survival rate.
Preferably, the matrix for improving HERBA DENDROBII training tissue culture seedling survival rate also includes organic nutrient, described organic The percentage by weight that nutrient accounts for matrix is 0.02%-0.05%.
Preferably, the organic nutrient includes inositol, vitamin B1, vitamin B6, glycine, nicotinic acid.Nutritive value is high Easily absorb, improve emergence rate or the seeding stage shifts to an earlier date, reduce and cultivate cost.
Preferably, the inositol, vitamin B1, vitamin B6, glycine, the volume ratio of nicotinic acid are:Inositol:Vitamin B1:Vitamin B6:Glycine:Nicotinic acid=150:1:1:4:1.Business ratio optimization, easily absorbs, and improves emergence rate or emergence Phase shifts to an earlier date, and reduces and cultivates cost.
The present invention still has numerous embodiments, all technical sides formed using equivalents or equivalent transformation Case, it is within the scope of the present invention.

Claims (5)

  1. A kind of 1. matrix for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:Including fat tree skin, wood fragments bits, leech Stone, decay sheep excrement, the volume ratio including fat tree skin, wood fragments bits, vermiculite, decay sheep excrement are:Fat tree skin:Wood fragments are considered to be worth doing:Vermiculite: Decay sheep excrement=8:4:1:0.5.
  2. 2. the matrix according to claim 1 for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:It is described to carry The matrix of high HERBA DENDROBII training tissue culture seedling survival rate also includes carbendazim, and the percentage by weight that the carbendazim accounts for matrix is 0.3%-0.5%.
  3. 3. the matrix according to claim 1 for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:It is described to carry The matrix of high HERBA DENDROBII training tissue culture seedling survival rate also includes organic nutrient, and the organic nutrient accounts for the percentage by weight of matrix For 0.02%-0.05%.
  4. 4. the matrix according to claim 3 for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:It is described to have Machine nutrient includes inositol, vitamin B1, vitamin B6, glycine, nicotinic acid.
  5. 5. the matrix according to claim 4 for improving HERBA DENDROBII training tissue culture seedling survival rate, it is characterised in that:The flesh Alcohol, vitamin B1, vitamin B6, glycine, the volume ratio of nicotinic acid are:Inositol:Vitamin B1:Vitamin B6:Glycine:Nicotinic acid =150:1:1:4:1.
CN201711070937.6A 2017-11-03 2017-11-03 A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate Pending CN107619353A (en)

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CN201711070937.6A CN107619353A (en) 2017-11-03 2017-11-03 A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate

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CN201711070937.6A CN107619353A (en) 2017-11-03 2017-11-03 A kind of matrix for improving HERBA DENDROBII training tissue culture seedling survival rate

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104145794A (en) * 2014-08-15 2014-11-19 湖南省农业信息与工程研究所 Temporary planting and seedling hardening method of dendrobium tissue culture seedlings

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104145794A (en) * 2014-08-15 2014-11-19 湖南省农业信息与工程研究所 Temporary planting and seedling hardening method of dendrobium tissue culture seedlings

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Address after: 224100 Jiangsu Yancheng City Dafeng Dafeng Port Economic Zone Jiangsu Ocean Industry Research Institute building

Applicant after: Institute of Dafeng Marine Industry, Nanjing University of Technology

Address before: 224100 Jiangsu Yancheng City Dafeng Port Economic Zone Jiangsu Ocean Industry Research Institute building

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Application publication date: 20180123