CN107616177B - Composition containing ZJ10520 and pyrrole bactericide - Google Patents

Abstract

The invention discloses a composition containing ZJ10520 and pyrrole bactericide and a bactericide thereof, wherein the composition has the advantages of obviously improved bactericidal effect compared with a single bactericide, good curative and protective effects, low medication cost, capability of slowing down the generation of resistance and prolonging the service life of the bactericide.

Description

Composition containing ZJ10520 and pyrrole bactericide

Technical Field

The invention belongs to the field of pesticides, and relates to a bactericidal composition for preventing and treating agricultural diseases.

Background

In agricultural production processes, fungal diseases are a major factor affecting yield and quality. In order to improve the yield and quality of crops, pesticides for preventing and treating diseases need to be used, and a bactericide is one of the pesticides. Commonly used bactericides can be classified into amides, dihydroxy imides, strobilurins, triazoles, imidazoles, oxazoles, thiazoles, morpholines, pyrroles, pyridines, pyrimidines, quinolines, carbamates, organophosphates, antibiotics and the like according to the chemical structure type. Wherein the pyrrole fungicide is a variety with a large using amount, and mainly comprises fludioxonil and fenpiclonil.

Fludioxonil (fludioxonil), chemical name: 4- (2, 2-difluoro-1, 3-benzodioxyl-4-yl) pyrrole-3-nitrile belongs to pyrrole contact fungicides, and the action mechanism of the pyrrole contact fungicides is that the transfer related to phosphorylation of glucose is inhibited, the growth of fungal mycelium is inhibited, and finally, the germs die. The composition can be used as stem and leaf treating agent and seed treating agent, and can be used as seed treating agent for preventing and treating seed-borne and soil-borne diseases such as Alternaria, Septoria, Aspergillus, Fusarium, Helminthosporium, Rhizoctonia, Penicillium, etc. Fludioxonil has no systemic properties.

Fenpiclonil (fenpiclonil), chemical name: the 4- (2, 3-dichlorophenyl) pyrrole-3-nitrile is a protective bactericide, is mainly used for preventing and controlling diseases of crops such as wheat, barley, corn, cotton, soybean, peanut, rice, rape, potato, vegetables and the like, has special effect on pathogenic bacteria of cereal crops in seed treatment, and also has good prevention and control effect on seed-borne and soil-borne diseases of non-cereal crops.

However, long-term use of the same variety of bactericide can cause the pesticide resistance of the pathogenic bacteria of the crops to be generated and reduce the control effect. For example, crop pathogens have developed some resistance to fludioxonil and fenpiclonil. In order to cope with the drug resistance of pathogenic bacteria, the sterilization active compounds with different action mechanisms are compounded to be a better method.

Therefore, the compounding of the pyrrole bactericide and other bactericides is a way to solve the drug resistance of germs.

Disclosure of Invention

The invention aims to provide a composition which has the characteristics of good sterilization effect, low medication cost and difficult generation of drug resistance.

The invention provides the following technical scheme:

a composition comprises two active ingredients, wherein the first active ingredient is ZJ10520, and the second active ingredient is selected from pyrrole bactericides.

The first active ingredient used in the present invention is ZJ10520, whose chemical name is: 3- (difluoromethyl) -5-fluoro-1-methyl-N- [2- (2-chloro-4-trifluoromethyl-phenoxy) phenyl ] pyrazole-4-carboxamide.

The second active ingredient used in the present invention is selected from azole fungicides. The pyrrole bactericide is a pyrrole bactericide commonly used in the industry.

Preferably, the azole fungicide is at least one selected from the group consisting of fludioxonil and fenpiclonil. When the azole fungicide is fludioxonil, the composition contains ZJ10520 and fludioxonil. When the pyrrole bactericide is fenpiclonil, the composition contains ZJ10520 and fenpiclonil. When the pyrrole bactericide is fludioxonil and fenpiclonil, the composition contains ZJ10520, fludioxonil and fenpiclonil.

The composition provided by the invention does not need to be specially matched with the two active ingredients.

Preferably, the mass ratio of the first active ingredient to the second active ingredient is 60: 1-1: 60.

More preferably, the mass ratio of the first active ingredient to the second active ingredient is 20: 1-1: 20.

More preferably, the mass ratio of the first active ingredient to the second active ingredient is 6: 1-1: 6.

Most preferably, the mass ratio of the first active ingredient to the second active ingredient is 3: 1-1: 3.

The composition provided by the invention can further comprise a third active ingredient. The third active component can be at least one selected from triazole bactericides, imidazole bactericides, oxazole bactericides, amide bactericides, dicarboximide bactericides, thiazole bactericides, pyrimidine bactericides, morpholine bactericides, pyridine bactericides, strobilurin bactericides, quinoline bactericides, organophosphorus bactericides, carbamate bactericides and antibiotic bactericides.

The composition provided by the invention is suitable for sterilization, and is particularly suitable for agricultural sterilization.

Preferably, the composition provided by the invention is suitable for controlling at least one selected from powdery mildew, damping off, gray mold, smut, snow rot, snow mold, banded sclerotial blight, root rot, take-all, glume blight, stalk smut, stripe disease, net blotch, bacterial wilt, stem rot, damping-off, red rot, anthracnose, black root disease, rot, bakanae disease, rice blast, black spot, black shank, blight, gummy stem blight, rust disease, scab, rust disease, leaf spot, downy mildew, epidemic disease, leaf blight, brown spot, ring spot, sclerotinia rot, fusarium wilt and gibberellic disease.

Further preferably, the composition provided by the invention is suitable for controlling at least one selected from rice sheath blight disease, brown spot of lawn, wheat sheath blight, vegetable damping-off and chrysanthemum gray mold.

The invention also provides a bactericide which comprises more than 0.1% by weight of the composition.

Preferably, the bactericide contains 1 to 90% by weight of the composition.

It is further preferred that the bactericide comprises 2 to 80% by weight of the composition.

The bactericide provided by the invention can further comprise agriculturally acceptable carriers and auxiliaries besides the composition.

The carrier of the present invention may be a solid or a liquid, and any carrier commonly used for formulating agricultural pharmaceutical compositions can be used.

Suitable solid supports include: minerals, plants, synthetic fillers and inorganic salts. Wherein the minerals include silicates, carbonates, sulfates and oxides. Silicates such as kaolin, sepiolite, nacrite, montmorillonite, mica, vermiculite, pyrophyllite, talc. Carbonates such as: calcium carbonate and dolomite. Sulfates such as ammonium sulfate, sodium sulfate, calcium sulfate. Oxides such as quicklime, magnesia lime, diatomaceous earth. Plants such as: citrus pulp, corn cob cores, rice hull powder, rice hulls, soybean straw powder, tobacco powder, walnut shells and sawmilling powder. Synthetic fillers such as precipitated calcium carbonate hydrate, precipitated calcium carbonate, white carbon black. Inorganic salts such as potassium chloride, sodium chloride.

The liquid carrier includes water and an organic solvent. When the active ingredient is a suspoemulsion, the organic solvent plays a role in solubilization and freeze protection. Suitable organic solvents include aromatic hydrocarbons such as benzene, xylene, toluene, alkylbenzenes, alkylnaphthalenes, and chlorinated aromatic hydrocarbons; chlorinated aliphatic hydrocarbons such as vinyl chloride, chloroform, methylene chloride, chloroform, carbon tetrachloride and polychlorinated ethane; aliphatic hydrocarbons such as petroleum fractions, cyclohexane, light mineral oil and paraffin wax. Alcohols such as methanol, ethanol, isopropanol, butanol, isobutanol, ethylene glycol, propylene glycol, glycerol, fatty alcohols, and the like; ethers such as methyl glycol ether, ethyl glycol ether, petroleum ether. Ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, and isophorone, N-methyl-pyrrolidone; the special solvent also comprises dimethylformamide, dimethyl sulfoxide, polyethylene glycol and hexanenitrile; vegetable oils and methylated vegetable oils. The organic solvents mentioned above may be used alone, or in admixture with water.

The auxiliary agent can comprise one or more of a surfactant, an antifoaming agent, a thickening agent, a suspending agent and an antifreezing agent according to needs, and can also comprise other auxiliary agents commonly used in the industry according to needs.

The surfactant can be an emulsifier, dispersant, stabilizer or wetting agent; may be ionic or non-ionic. Suitable surfactants include: sodium and calcium salts of polyacrylic acid and lignosulfonic acid; polycondensation products of fatty acids or fatty amines containing at least 12 carbon atoms in the molecule with ethylene oxide and/or propylene oxide; fatty acid esters of glycerol, dodecanol-1, tetradecanol-1, sorbitol, sucrose, or pentaerythritol; and their polycondensation products with ethylene oxide and/or propylene oxide; sulfates or sulfonates of their polycondensation products; alkali metal or alkaline earth metal salts of sulfuric acid or sulfonic acid having at least 10 carbon atoms in the molecule, preferably sodium salts such as sodium lauryl sulfate, sodium secondary alkyl sulfate, sodium salt of sulfonated castor oil, sodium alkylaryl sulfonate and sodium dodecylbenzene sulfonate; polymers of ethylene oxide, copolymers of ethylene oxide and propylene oxide.

The emulsifier includes nonionic emulsifier and anionic emulsifier. The nonionic emulsifier is preferably nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, styrylphenyl polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, hydroxyl-terminated polyoxyethylene polyoxypropylene ether, styrylphenol formaldehyde resin polyoxyethylene polyoxypropylene ether, and castor oil polyoxyethylene ether. The anionic emulsifier mainly comprises calcium dodecyl benzene sulfonate, ammonium triphenethyl phenol polyoxyethylene ether phosphate, ammonium nonylphenol polyoxyethylene ether phosphate and ammonium castor oil polyoxyethylene ether phosphate.

The dispersant of the invention comprises: one or more of acrylic acid homopolymer sodium salt, maleic acid disodium salt, naphthalene sulfonic acid formaldehyde condensate sodium salt, rosin block polyoxyethylene ether polyoxypropylene ether sulfonate, hydroxyl-terminated polyoxyethylene polyoxypropylene ether block copolymer, triphenyl ethyl phenol polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphoric acid and p-hydroxyphenyl lignin sulfonic acid sodium salt.

The humectants of the present invention include: one or more of fatty alcohol-polyoxyethylene ether, naphthalene sulfonate, sodium dodecyl sulfate and alkylphenol resin polyoxyethylene ether sulfate.

The thickening agent comprises one or more of xanthan gum, magnesium aluminum silicate, sodium alginate, sodium carboxymethylcellulose, Arabic gum, gelatin and polyvinyl alcohol.

The defoaming agent of the present invention is preferably: molinate, silicones, C_8～10Fatty alcohol, C_10～20Saturated fatty acids, amides, and the like.

The fungicide according to the present invention can be formulated into any agriculturally acceptable dosage form as required.

Preferably, the bactericide may be formulated into a powdery preparation, a granular preparation, a dispersible powdery preparation, a dispersible granular preparation, a dispersible tablet preparation, a soluble solid preparation, a soluble liquid preparation, an oil preparation, an ultra-low volume preparation, a dispersible liquid preparation, an emulsion preparation, a suspension preparation, a suspoemulsion preparation or a seed coating preparation.

Preferably, the powdered formulation is selected from a powder, a contact powder or a floating powder.

Preferably, the granular formulation is selected from granules, macrogranules, fine granules, microgranules or microencapsulated granules.

Preferably, the dispersible powder formulation is selected from a wettable powder or an oil dispersible powder.

Preferably, the dispersible granular formulation is selected from water dispersible granules, milk granules or effervescent granules.

Preferably, the dispersible tablet formulation is selected from a dispersible tablet or an effervescent tablet.

Preferably, the soluble solid formulation is selected from soluble powders, soluble granules or soluble tablets.

Preferably, the soluble liquid formulation is selected from a solubles, a water aqua, or a sol.

Preferably, the oil formulation is selected from an oil or a spreading oil.

Preferably, the ultra-low volume formulation is selected from an ultra-low volume liquid formulation or an ultra-low volume microcapsule suspension.

Preferably, the dispersible liquid formulation is selected from emulsifiable concentrates or dispersible liquid formulations.

Preferably, the emulsion formulation is selected from an aqueous emulsion, an oil emulsion or a microemulsion.

Preferably, the suspending agent is selected from a suspending agent, a microcapsule suspending agent or an oil suspending agent.

Preferably, the seed coating is selected from an aqueous suspension seed coating, a dispersible powder seed coating or a dispersible granule seed coating.

Compared with the prior art, the composition and the bactericide have the following advantages:

(1) the two active ingredients in the composition are compounded to show excellent synergistic effect, the sterilization effect of the mixed composition is obviously improved compared with that of a single agent, and the composition has good sterilization effect and treatment and protection effects;

(2) the dosage and the cost are reduced;

(3) the two active ingredients are reasonably mixed by different action modes and different action mechanisms, so that the service life of the bactericide is prolonged, the generation of resistance is slowed down, the safety to crops is good, and the safety requirement of pesticide preparations is met.

Detailed Description

The present invention is further illustrated by the following examples, which are not intended to limit the invention to these embodiments. It will be appreciated by those skilled in the art that the present invention encompasses all alternatives, modifications and equivalents as may be included within the scope of the claims.

First, bioassay example

Example 1, indoor toxicity assay of the Compound pairing of ZJ10520 and fludioxonil with Chrysanthemum Botrytis

Test subjects: botrytis cinerea (Botrytis cinerea) is preserved in the unit strain room.

The test method comprises the following steps: refer to the hypha growth rate method of agricultural industry Standard NY/T1156.2-2006 of the people's republic of China. Each medicament is respectively treated by 5 dosages according to the content of effective components, the pathogenic bacteria are cultured by a PDA culture medium, when bacterial colonies grow to a culture dish, a puncher with the diameter of 5mm is used for punching bacterial blocks at the edges of the bacterial colonies, the bacterial blocks are moved to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in a 25 ℃ culture box for culture, and the treatment is repeated for 4 times. According to the growth condition of CK colonies, the diameter cm of each treated colony is measured by a caliper by adopting a cross method, and the corrected inhibition percentage is calculated. The EC of each drug was determined by linear regression analysis between the probability of inhibition and the logarithm of the series of concentrations₅₀The co-toxicity coefficient (CTC) was then calculated according to the Sun cloud Peel method.

And (3) calculating the drug effect: two diameters were cross-measured per colony, and the average was used to represent colony size. The colony growth inhibition rate was determined according to the following formula: the colony growth inhibition ratio ═ g (blank colony growth diameter-agent-treated colony growth diameter) × 100/blank colony growth diameter.

The results were subjected to data analysis and statistics using the 3.11 professional edition to determine a regression line, EC50, and a correlation coefficient. EC50 for each treatment was converted to the Actual Toxicity Index (ATI) by the Sun cloud Peel (Y-P Sun); obtaining a Theoretical Toxicity Index (TTI) according to the mixture ratio of the mixture, and calculating the co-toxicity coefficient (CTC) of the mixture according to the following formula.

Theoretical virulence index of the mix (TTI) ═ A virulence index x A content in the mix (%)

+ B virulence index x B content in the mix (%)

If the co-toxicity coefficient is more than 120, the synergistic effect is shown; if the content is obviously lower than 100, antagonism is indicated; between 100 and 120, the additive effect is shown.

TABLE 1 indoor toxicity test results of chrysanthemum gray mold compounded by ZJ10520 and fludioxonil

Medicament	EC50(μg/mL)	ATI	TTI	Co-toxicity coefficient CTC
					ZJ10520(A)	3.27	100.00	/	/
Fludioxonil (B)	0.047	6957.45	/	/
					A+B(20：1)	0.561	582.89	426.55	136.65
A+B(16：1)	0.423	773.05	503.38	153.57
					A+B(12：1)	0.282	1159.57	627.50	184.79
A+B(6：1)	0.141	2319.15	1079.64	214.81
					A+B(3：1)	0.071	4605.63	1814.36	253.84
A+B(1：1)	0.042	7785.71	3528.73	220.64
					A+B(1：3)	0.032	10218.75	5243.09	194.90
A+B(1：6)	0.033	9909.09	5977.81	165.76
					A+B(1：12)	0.031	10548.39	6429.95	164.05
A+B(1：16)	0.034	9617.65	6554.07	146.74
					A+B(1：20)	0.039	8384.62	6630.90	126.45

Example 2, indoor virulence determination of ZJ10520 in combination with fludioxonil for wheat sheath blight and turf brown spot

Test subjects: wheat sharp eyespot (Rhizoctonia solani) and lawn brown spot (Rhizoctonia solasonii), were maintained in the unit seed chamber. Test methods and drug efficacy calculations refer to example 1.

Table 2, results of indoor toxicity determination of ZJ10520 and fludioxonil compounding on wheat sharp eyespot

Table 3, results of indoor toxicity measurement of lawn brown spot compounded by ZJ10520 and fludioxonil

Medicament	EC50(μg/mL)	ATI	TTI	Co-toxicity coefficient CTC
					ZJ10520(A)	0.0029	100.00	/	/
Fludioxonil (B)	0.0183	15.85	/	/
					A+B(20：1)	0.0025	116.00	95.99	120.84
A+B(16：1)	0.0023	126.09	95.05	132.65
					A+B(12：1)	0.0022	131.82	93.53	140.94
A+B(6：1)	0.0019	152.63	87.98	173.49
					A+B(3：1)	0.0016	181.25	78.96	229.54
A+B(1：1)	0.0018	161.11	57.93	278.14
					A+B(1：3)	0.0032	90.63	36.89	245.68
A+B(1：6)	0.0047	61.70	27.87	221.38
					A+B(1：12)	0.0071	40.85	22.32	182.97
A+B(1：16)	0.0092	31.52	20.80	151.55
					A+B(1：20)	0.011	26.36	19.86	132.77

Example 3, ZJ10520, indoor toxicity determination of fludioxonil and azoxystrobin ternary combination on rice sheath blight

Test subjects: wheat sharp eyespot (rhizoctoniaseni), test method and efficacy calculation reference example 1.

TABLE 4 indoor toxicity test results of ternary combination of fludioxonil and difenoconazole for rice sheath blight disease, ZJ10520

Second, preparation examples

The percentage contents in all the preparation ratios are mass percentages.

Example 4, 60% ZJ10520 fludioxonil Water dispersible granule

45% of ZJ10520, 15% of fludioxonil, 3% of TERSPERSE 2700, 2% of a dispersing agent NNO (alkyl naphthalene sulfonate formaldehyde condensate), 3% of nekal BX (sodium dibutylnaphthalene sulfonate), 4% of K-12 (sodium dodecyl sulfate), 3% of diatomite, 5% of glucose and kaolin are weighed to be added to 100%.

The preparation method of the water dispersible granule comprises the following steps: according to the formula proportion, the raw medicine, the dispersing agent, the wetting agent, the binding agent and the like are mixed and subjected to superfine airflow crushing by a conventional method for preparing water dispersible granules, and the mixture is added into a granulator with a screen with a certain specification for granulation. Then drying and screening to obtain granular products.

Example 5, 50% ZJ10520 fludioxonil wettable powder

Weighing 25% of ZJ10520, 25% of fludioxonil, 5% of calcium lignosulfonate, 2% of nekal BX, 1% of K-12, 5% of white carbon black and kaolin to 100% by mass.

The preparation method of the wettable powder comprises the following steps: according to the formula requirement, after being mixed, the mixture is crushed by a coarse crusher and then enters a jet mill to be fully crushed and mixed to prepare the wettable powder.

Example 6, 2% ZJ10520 seed-Rol suspension seed coating

0.5% of ZJ10520, 1.5% of fenpiclonil, 1.6% of polyvinyl alcohol (PVA), 0.4% of homopolymerized vinyl acetate, 1.0% of nonylphenol polyoxyethylene ether (NP-10), 3.0% of nonylphenol polyoxyethylene ether phosphate, 5.0% of ethylene glycol, 0.01% of 1, 2-benzisothiazolin-3-one (BIT), 0.3% of organic modified siloxane antifoaming agent, 0.1% of xanthan gum, 1.5% of alkaline rose essence and the balance of water are weighed. Mixing to obtain 2% ZJ10520 seed-chuck suspension seed coating agent.

Example 7, 10% ZJ10520 suspension

Weighing 10% of ZJ10520, 4% of acrylic acid homopolymer sodium salt, 1% of fatty alcohol-polyoxyethylene ether, 3% of propylene glycol, 0.3% of organic silicone oil, 0.2% of potassium sorbate, 0.15% of xanthan gum and water to be supplemented to 100%. Mixing to obtain 10% ZJ10520 suspending agent.

Third, field application example

Example 8 field efficacy test for prevention and treatment of Rice sheath blight disease

The field efficacy test for preventing and treating the rice sheath blight refers to the regulation of pesticide field efficacy test criterion (I) GB/T17980.20-2000 pesticide field efficacy test institute of Ministry of agriculture, namely that the bactericide prevents and treats the rice sheath blight, the cell treatment of the test medicament, the control medicament and the blank control adopts random block arrangement, the cell area is 15m2, the 4 times of repetition are carried out, and the test is carried out twice in total. The first administration is at the initial stage of onset, and the second administration is given one week later. The test is carried out for 2 times in total, five points of diagonal lines of each test cell are sampled, 5 connected clusters and 25 clusters are investigated at each point, and the total number of plants, the number of diseased plants and the number of diseased stages are recorded.

Disease grading criteria are as follows:

level 0: the whole plant is disease-free;

level 1: the fourth leaf and the leaf sheaths and leaves below the fourth leaf (the first sword leaf is taken as the first leaf);

and 3, level: the third leaf and the leaf sheaths and leaves below the third leaf are attacked;

and 5, stage: the second leaf and the leaf sheaths and leaves below the second leaf are attacked;

and 7, stage: attack of Jianye leaf and the leaf sheaths and leaves below the Jianye leaf;

and 9, stage: the whole plant is attacked and withered in advance.

The drug effect calculation method comprises the following steps:

according to the investigation result, the disease index and the prevention effect are calculated according to the following formulas (1) and (2). The test data were statistically analyzed by the Duncan's New Complex Pole Difference Method (DMRT).

In the formula: CK (CK)₀、CK₁Disease indexes before and after drug application in the blank control area are respectively; PT₀、PT₁The disease indexes before and after the medicine treatment area respectively.

TABLE 5 field test results of efficacy in preventing and treating sheath blight of rice

Example 9 field efficacy test for controlling tomato damping-off

Uniformly coating tomato seeds and 2 percent of ZJ10520 & fenpiclonil suspension seed coating agent according to the ratio of the seeds to the seeds of the tomato being 1: 800. Sowing the seeds 3 days after coating, sowing the seeds in pots with the inner diameter of 6.5cm and the height of 6cm (2 seeds are sowed in each pot), then placing the pots in a greenhouse for conventional management until the leaf stage reaches 3, taking uncoated seeds as blank control, and repeating the steps for 4 times. The fusarium wilt bacteria are mashed and inoculated on the base of the stem. And (4) carrying out grading investigation according to the requirement of pesticide field efficacy test criterion (II) (GB/T17980.113-2004). Observing the influence of the seed coating agent on seedling emergence and the control effect of tomato damping-off.

TABLE 6, 2% ZJ 10520. prevention of tomato damping off with seed-pyrrole suspension seed coating agent

Treatment of	Damping off (%)	Rate of emergence (%)	Injury from drug
				The medicine seed ratio is 1:800	90.88	92.11	Is free of
Blank control	-	87.3	Is free of

The embodiments show that the composition provided by the invention can well prevent and treat rice sheath blight disease, lawn brown spot, wheat sheath blight, vegetable damping-off and chrysanthemum gray mold, the activity and the bactericidal effect of the composition are not simple superposition of the activities of all components, but have obvious synergistic effect, and meanwhile, due to the increase of action sites, the composition is beneficial to overcoming and delaying the generation of drug resistance of germs. In addition, the composition provided by the invention has no obvious adverse effect on test crops, has normal leaf color, growth vigor and the like, has good safety and meets the safety requirement of pesticide preparations.

Claims (11)

1. A composition characterized in that it comprises two active ingredients, the first active ingredient being ZJ10520, chemically named: the composition comprises 3- (difluoromethyl) -5-fluoro-1-methyl-N- [2- (2-chloro-4-trifluoromethyl-phenoxy) phenyl ] pyrazole-4-formamide and a second active ingredient, wherein the second active ingredient is selected from a pyrrole bactericide, the mass ratio of the first active ingredient to the second active ingredient is 60: 1-1: 60, and the pyrrole bactericide is selected from at least one of fludioxonil and fenpiclonil.

2. The composition according to claim 1, wherein the mass ratio of the first active ingredient to the second active ingredient is 20: 1-1: 20.

3. The composition according to claim 2, wherein the mass ratio of the first active ingredient to the second active ingredient is 6:1 to 1: 6.

4. The composition according to claim 3, wherein the mass ratio of the first active ingredient to the second active ingredient is 3:1 to 1: 3.

5. A fungicide characterized by containing, by weight, more than 0.1% of the composition according to any one of claims 1 to 4.

6. The fungicide according to claim 5, characterized in that said fungicide contains said composition in an amount of 1% to 90% by weight.

7. The fungicide according to claim 6, characterized in that said fungicide contains 2% to 80% by weight of said composition.

8. The fungicide according to claim 5, characterized in that the fungicide is formulated into a powdery preparation, a granular preparation, a dispersible powdery preparation, a dispersible granular preparation, a dispersible tablet preparation, a soluble solid preparation, a soluble liquid preparation, an oil preparation, an ultra-low volume preparation, a dispersible liquid preparation, an emulsion preparation, a suspension preparation, a suspoemulsion preparation or a seed coating preparation.

9. The bactericide as claimed in claim 8, wherein: the powdery preparation is selected from powder, contact powder or floating powder, the granular preparation is selected from granules, large granules, fine granules, micro granules or micro capsule granules, the dispersible powdery preparation is selected from wettable powder or oil dispersible powder, the dispersible granular preparation is selected from water dispersible granules, emulsion granules or effervescent granules, the dispersible tablet preparation is selected from dispersible tablets or effervescent tablets, the soluble solid preparation is selected from soluble powder, soluble granules or soluble tablets, the soluble liquid preparation is selected from soluble solution, water aqua or soluble gum agent, the oil preparation is selected from oil solution or film-spreading oil solution, the ultra-low volume preparation is selected from ultra-low volume liquid preparation or ultra-low volume micro capsule suspension, the separable liquid preparation is selected from missible oil or dispersible solution, the emulsion preparation is selected from emulsion in water, oil emulsion or microemulsion, the suspension preparation is selected from suspension, oil emulsion in water, or microemulsion, The microcapsule suspension or oil suspension is selected from water suspension seed coating, dispersible powder seed coating or dispersible granule seed coating.

10. Use of a composition according to claim 1 for controlling at least one selected from the group consisting of damping off, gray mold, black ear, snow rot, snow mold, sheath blight, root rot, take-all, glume blight, stalk smut, stripe disease, net blotch, bacterial wilt, stem rot, damping off, red rot, anthracnose, black root, rot, bakanae disease, rice blast, black spot, black shank, blight, gummy stem blight, rust, scab, rust, leaf spot, downy mildew, blight, leaf blight, brown spot, ring spot, sclerotinia, fusarium wilt and gibberellic disease.

11. Use of a composition according to claim 10, characterized in that the composition is used for controlling at least one selected from the group consisting of rice sheath blight disease, lawn brown spot disease, wheat sheath blight disease, vegetable damping off and chrysanthemum gray mold.