CN110573016B

CN110573016B - Fungicidal compositions and their use

Info

Publication number: CN110573016B
Application number: CN201880025098.2A
Authority: CN
Inventors: J·T·布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2017-05-04
Filing date: 2018-04-16
Publication date: 2022-04-15
Anticipated expiration: 2038-04-16
Also published as: GB2562083A; WO2018201883A1; CN110573016A; GB201707096D0; AR111376A1; GB2562083B

Abstract

The invention provides a fungicidal composition which comprises boscalid, dimethomorph and picoxystrobin. Also provided are methods of preventing, treating and/or controlling a fungal infection in a plant of interest, comprising applying to the plant, plant part or surroundings thereof boscalid, dimethomorph and picoxystrobin. The combination of boscalid, dimethomorph and picoxystrobin showed a significant synergistic effect in the treatment of a wide range of fungal infections.

Description

Fungicidal compositions and their use

The present invention relates to fungicidal compositions comprising a synergistic combination of three active components. The present invention also relates to methods of preventing, controlling and treating fungal infections using combinations of the foregoing active ingredients.

Fungicides are natural or synthetic compounds used to protect plants from fungal infections. Current practice in agriculture relies on the use of fungicides. In fact, many crops cannot grow efficiently without the use of certain fungicides. By using fungicides, farmers can increase crop yield and thus crop value. In most cases, the value gain of the crop due to the fungicide is many times higher than the cost of applying the fungicide to the crop.

However, there are no fungicides that can be applied to all cases of fungal infections. In addition, repeated use of the same type of fungicide will cause the fungus infecting the target plant to develop resistance to this type or other related types of fungicides.

Accordingly, there is an ongoing effort in the art to develop and produce fungicides and fungicidal compositions having greater safety, improved performance, lower cost, lower dosage rates, and greater convenience and ease of use. The ability to reduce the fungicide dosage rate required to achieve a desired level of control helps reduce the development of resistance by the target fungus.

Boscalid, dimethomorph and picoxystrobin are known fungicidally active compounds and are commercially available as fungicidal preparations. It has surprisingly been found that the combination of boscalid, dimethomorph and picoxystrobin shows a synergistic effect in the prevention and treatment of fungal infections.

According to a first aspect of the present invention, there is provided a fungicidal composition comprising boscalid, dimethomorph and picoxystrobin.

In a further aspect, the present invention also provides a method of preventing, treating and/or controlling fungal infection in a plant of interest, the method comprising applying boscalid, dimethomorph and picoxystrobin to the plant, part of the plant or surroundings thereof.

In a still further aspect, the present invention provides the use of a combination of boscalid, dimethomorph and picoxystrobin for the prevention, treatment or control of a fungal infection in a plant of interest.

Boscalid is a nicotinamide fungicide successfully developed by BASF, germany. The chemical name of boscalid is 2-chloro- Ν - (4' -chlorobiphenyl-2-yl) nicotinamide. It has a broad fungicidal spectrum and exhibits preventive effects. Although shown to be active against almost all types of fungal diseases, it is particularly effective in controlling powdery mildew, gray mold, root rot, sclerotinia and various rot diseases, and also effective against fungal infections that exhibit resistance to other agents. The boscalid is mainly used for controlling fungal diseases of rape, grapes, fruit trees, vegetables, field crops and the like.

Boscalid is a succinate dehydrogenase inhibitor (SDHI) which belongs to the class of mitochondrial respiration inhibitors and acts by inhibiting succinate coenzyme Q reductase (also known as compound II) in the mitochondrial electron transport chain.

Dimethomorph is a morpholine fungicide and has the chemical name (E, Z) -4- [3- (4-chlorophenyl) -3- (3, 4-dimethoxyphenyl) acryloyl ] morpholine. Dimethomorph is effective against oomycetes, in particular the peronosporaceae (Peronosporaceae) and Phytophthora species (Phytophthora spp.). The compounds can be used for preventing or treating infection caused by fungal pathogens in crops such as grapes, cucumbers, melons, balsam pears, tomatoes, peppers, potatoes, cruciferous vegetables and the like.

Dimethomorphs act by preventing the formation of or causing damage to the cell wall of the target fungus.

Picoxystrobin is a strobilurin fungicide of the strobilurin class and has the chemical name methyl (2E) -3-methoxy-2- {2- [6- (trifluoromethyl) -2-pyridyloxymethyl ] phenyl } acrylate. Picoxystrobin is a systemic broad-spectrum fungicide used to control wheat leaf diseases (e.g., leaf blight, leaf rust, glume blight, brown spot, powdery mildew, etc.).

The inventors have surprisingly found that the combined application of boscalid, dimethomorph and picoxystrobin in one composition or separately results in significantly better harmful fungal control than the application of each of the above compounds alone. More specifically, the activity and/or other desirable properties achieved by the combination of boscalid, dimethomorph and picoxystrobin according to the invention are higher than expected based on the individual components boscalid, dimethomorph or picoxystrobin, indicating a significant synergistic effect.

The boscalid, dimethomorph and picoxystrobin combination of the invention allows the application dosage rates of these components to be significantly reduced and thus the application costs to be reduced, while still achieving the desired level of control. The combination of boscalid, dimethomorph and picoxystrobin broadens the control spectrum of the fungicide, delays the generation and development of fungal pathogen resistance and shows obvious synergistic effect. The three active ingredients do not produce cross-resistance.

As indicated above, in one aspect of the present invention, there is provided a composition comprising boscalid, dimethomorph and picoxystrobin.

Boscalid may be present in the composition in any suitable amount to provide a synergistic effect with dimethomorph and picoxystrobin. The amount of boscalid in the composition may range from 1% to 90% by weight of the composition, preferably from 1% to 80%, more preferably from 1% to 70%, yet more preferably from 5% to 60%, yet more preferably from 10% to 60% by weight. In some preferred embodiments, boscalid is present in an amount from 5% to 55% by weight of the composition, more preferably from 10% to 55%, still more preferably from 10% to 50%, in particular from 10% to 45% by weight of the composition.

The dimethomorph may be present in the composition in any suitable amount to provide a synergistic effect with boscalid and picoxystrobin. The amount of dimethomorph in the composition may range from 1% to 90% by weight of the composition, preferably from 1% to 80%, more preferably from 1% to 70%, yet more preferably from 1% to 60%, yet more preferably from 5% to 60% by weight. In some preferred embodiments, the dimethomorph is present in an amount of from 1% to 55% by weight of the composition, more preferably from 1% to 50%, yet more preferably from 5% to 50%, especially from 5% to 45% by weight of the composition.

Picoxystrobin may be present in the composition in any suitable amount to provide a synergistic effect with boscalid and dimethomorph. The amount of picoxystrobin in the composition may range from 1% to 90% by weight of the composition, preferably from 1% to 80%, more preferably from 1% to 70%, still more preferably from 1% to 60%, still more preferably from 5% to 60% by weight. In some preferred embodiments picoxystrobin is present in an amount of from 5% to 55% by weight of the composition, more preferably from 5% to 50%, still more preferably from 5% to 45%, in particular from 5% to 40% by weight of the composition.

According to a preferred embodiment of the present invention, the composition comprises from 10 to 60% by weight boscalid, from 5 to 45% by weight dimethomorph, and from 5 to 40% by weight picoxystrobin.

The compositions of the present invention may be prepared in conventional manner and provided in any suitable formulation, for example by mixing boscalid, dimethomorph and picoxystrobin together with one or more adjuvants suitable for the type of formulation.

The compositions of the present invention may contain one or more adjuvants, depending on the requirements of the particular formulation. Suitable adjuvants which may be included in the compositions of the present invention are conventional formulation adjuvants or components such as one or more extenders, carriers, solvents, surfactants, stabilizers, defoamers, antifreeze agents, preservatives, antioxidants, colorants, thickeners, solid adhesion agents and inert fillers. Such adjuvants are known in the art and are commercially available. Their use in the formulation of the compositions of the present invention will be clear to one of ordinary skill in the art.

The preparation types suitable for the composition comprise soluble Solution (SL), missible oil (EC), aqueous Emulsion (EW), Microemulsion (ME), suspending agent (SC), oil suspending agent (OD), suspended seed coating agent (FS), water dispersible granule (WG), Soluble Granule (SG), Wettable Powder (WP), Soluble Powder (SP), Granule (GR), micro-Capsule Granule (CG), Fine Granule (FG), large granule (GG), aqueous Suspoemulsion (SE), micro-capsule suspending agent (CS) and micro-granule (MG). The following paragraphs will describe exemplary formulations of fungicide compositions, including water dispersible granules (WG), suspending agents (SC), Wettable Powders (WP), and Soluble Granules (SG).

The fungicidal composition may comprise one or more inert fillers. Such inert fillers are known in the art and are commercially available. Suitable fillers include, for example, natural ground minerals such as kaolin, alumina, talc, chalk, quartz, attapulgite, montmorillonite, and diatomaceous earth; or synthetic ground minerals such as highly dispersed silicic acid, alumina, silicates, and calcium hydrogenphosphates. Suitable inert fillers for the granules include, for example, crushed and classified natural minerals (such as calcite, marble, pumice, sepiolite, and dolomite), or synthetic granules of inorganic and organic abrasive materials, as well as granules of organic materials (such as sawdust, coconut shells, corn cobs, and tobacco stalks).

The fungicidal compositions of the present invention optionally include one or more surfactants, which are preferably nonionic, cationic and/or anionic in nature, and are mixtures of surfactants with good emulsifying, dispersing and wetting properties, depending on the nature of the active compound to be formulated. Suitable surfactants are known in the art and are commercially available.

The surfactant may be an emulsifier, dispersant or wetting agent of ionic or non-ionic type. Examples which may be used are salts of polyacrylic acids; a lignosulfonate; salts of benzenesulfonic acid or naphthalenesulfonic acid; polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines; substituted phenols, especially alkylphenols; sulfosuccinate salts; taurine derivatives, especially alkyl taurates; or a phosphate ester of a polyethoxylated phenol or alcohol. The presence of at least one surfactant is generally required when the active compound and/or inert carrier and/or adjuvant/adjuvant is insoluble in water and the final application vehicle for the composition is water.

Examples of suitable surfactants are Polyoxyethylated (POE) sorbitan esters, such as POE (20) sorbitan trioleate and Polyoxyethylated (POE) sorbitol esters, such as POE (40) sorbitol hexaoleate. POE (20) sorbitan trioleate is commercially available under the trade names ATLAS G1086 and CIRRASOL G1086 sold by UniqEMA. The combination of POE sorbitan ester with POE sorbitol ester allows to optimize the HLB (hydrophilic lipophilic balance) value of the surfactant in order to obtain the highest quality aqueous emulsion (minimum suspended droplets) when the composition is added to water. Higher quality aqueous emulsions typically achieve optimal fungicidal performance.

Suitable anionic surfactants may be both so-called soluble soaps and soluble synthetic surface active compounds. The soap which can be used in the composition is an alkali metal, an alkaline earth metal or a higher fatty acid (C)₁₀-C₂₂) Substituted or unsubstituted ammonium salts of, for example, oleic or stearic acid, sodium or potassium salts, or natural fatty acidsSodium or potassium salts of the compounds.

The amount of surfactant present in the composition will depend on such factors as the type of formulation used.

The fungicidal compositions of the present invention optionally further comprise one or more polymeric stabilizers. Suitable polymeric stabilizers that may be used in the present invention include, but are not limited to: polypropylene, polyisobutylene, polyisoprene, copolymers of monoolefins and diolefins, polyacrylates, polystyrene, polyvinyl acetate, polyurethanes or polyamides. Suitable stabilizers are known in the art and are commercially available.

It is generally believed that the surfactants and polymeric stabilizers described above impart stability to the composition, thereby allowing the composition to be formulated, stored, transported, and applied.

Suitable antifoams include all materials which normally can be used for this purpose in agrochemical compositions. Suitable defoamers are known in the art and are commercially available. Particularly preferred defoamers are mixtures of polydimethylsiloxanes and perfluoroalkyl phosphoric acids, such as silicone defoamers available from GE or Compton.

Suitable organic solvents for use in these compositions may be selected from all conventional organic solvents which adequately dissolve one or more of the active compounds employed. Suitable organic solvents for the active compounds in the compositions of the present invention are also known in the art. The following may be mentioned as preferred: n-methylpyrrolidone, N-octylpyrrolidone, cyclohexyl-1-pyrrolidone; or mixtures of paraffinic, isoparaffinic, cycloparaffinic and aromatic hydrocarbons, e.g. SOLVESSO^TM200. Suitable solvents are commercially available.

Suitable preservatives include all materials which may normally be used in agrochemical compositions of this type for this purpose and are likewise well known in the art. Suitable preservatives which may be mentioned include tolylfluanide, for example

Commercially available from Bayer AG and benzisothiazolinones, e.g.

(commercially available from Bayer AG).

Suitable antioxidants are all substances which can be normally used for this purpose in agrochemical compositions, as is known in the art. Butylated hydroxytoluene is preferred.

Suitable thickeners include all substances which can normally be used in agrochemical compositions for this purpose. Suitable thickeners include, for example, xanthan gum, PVOH, cellulose and its derivatives, hydrated silicate clays, magnesium aluminum silicate or mixtures thereof. Again, such thickeners are known in the art and are commercially available.

The fungicidal compositions of the present invention may further comprise one or more solid adhesion agents. Such adhesives are known in the art and are commercially available. They include organic binders including tackifiers such as cellulose or substituted cellulose, natural and synthetic polymers in powder, granule, or lattice form, and inorganic binders such as gypsum, silica, or cement.

Furthermore, the composition of the present invention may further comprise water depending on the formulation.

Boscalid, dimethomorph and picoxystrobin may be present in the composition or used in the present invention in any weight ratio that provides the above synergistic effect. Preferably, boscalid, dimethomorph and picoxystrobin are used in the following weight ratios: from 0.5 to 8.0 boscalid, from 0.5 to 3.0 dimethomorph, from 0.5 to 3.0 picoxystrobin, more preferably from 0.7 to 7.5 boscalid, from 0.7 to 2.5 dimethomorph, from 0.7 to 2.5 picoxystrobin, still more preferably from 0.8 to 7.0 picoxystrobin, from 0.8 to 2.2 dimethomorph, from 0.8 to 2.0 picoxystrobin. Particularly preferred weight ratios of boscalid, dimethomorph and picoxystrobin are described in the specific examples listed below.

According to an embodiment of the invention, the weight ratio of boscalid to the combined amount of dimethomorph and picoxystrobin is from about 1:100 to 100:1, preferably from about 1:50 to 50:1, more preferably from about 1:25 to 25:1, still more preferably from about 1:10 to 10: 1.

The rates of application (use) of boscalid, dimethomorph and picoxystrobin in the compositions, methods and uses of the invention will vary depending on factors such as the type of use, the type of soil, the season, the climate, soil ecology, the type of plant, etc., but such that boscalid, dimethomorph and picoxystrobin are applied in effective amounts to provide the desired effect. The rate of application of the composition for a given set of conditions can be readily determined by conducting tests.

The rate of application of the total amount of boscalid, dimethomorph and picoxystrobin is generally in the range of from about 10 grams per hectare (g/ha) to about 9000g/ha, preferably from 20g/ha to 5000 g/ha. In general, beneficial results will be obtained when the following compounds are used: from about 5g/ha to about 3000g/ha, preferably from about 10g/ha to about 2000g/ha of boscalid; from about 5g/ha to about 1500g/ha, preferably from about 10g/ha to about 800g/ha, of dimethomorph; and from about 5g/ha to 1500g/ha, preferably from about 10g/ha to about 800g/ha of picoxystrobin.

Such formulations, as described above, can be used directly (i.e. undiluted) or after dilution with a suitable solvent (especially water), by spraying, pouring or immersion for treatment and protection of the plants, plant parts and/or the locus thereof from fungal infections. In general, it is preferred that these formulations can be diluted with water prior to administration. These compositions and formulations can be administered by methods known in the art. The method comprises coating, spraying, soaking, injecting, irrigating and the like.

The active ingredients may be applied simultaneously and/or continuously, preferably at short intervals (e.g. on the same day) to the plant, plant part and/or locus thereof which it is desired to control. The active ingredients may be applied to the plant, one or more parts of the plant (such as the leaves or seeds), and/or the locus thereof, in any order. Each component may be administered only once or multiple times. Preferably, each of the components is applied a plurality of times, in particular from 2 to 5 times. The active fungicidal components may be applied to the target plant, plant material or plant part or to the locus where the plant is growing, planted or is to be planted in any order.

The active ingredient may be administered in any suitable form as described above. Typically, as discussed above, the active ingredients will be administered as a formulation, i.e., a composition comprising one or more active ingredients together with additional carriers, surfactants, or other adjuvants conventionally used in formulation technology to facilitate administration.

In the case of simultaneous application of the active ingredients in the present invention, they may be applied as compositions comprising the fungicidal active ingredients, in which case the ingredients may be obtained from separate formulation sources and mixed together (referred to as tank mixes, ready-to-use forms, spray liquors, or slurries), optionally together with other pesticides), or the ingredients may be obtained as a single formulation mixture source (referred to as a premix, concentrate, formulated compound (or product)) and optionally mixed together with other pesticides), in particular as a composition according to the present invention.

The compositions, methods and uses of the present invention are useful for preventing and/or controlling fungal infections in a range of plants.

The fungicidal components may be applied to a plant (including plant propagation material, e.g. a seed) at any stage of growth, to the locus of the plant before germination (i.e. the period before the plant germinates from the soil), after germination (i.e. after the plant germinates from the soil) or in two or more of these stages. In one embodiment, the fungicidal component is applied to the plant during the pre-emergence and post-emergence stages (i.e., the stages between seedling emergence and plant maturation). In one embodiment, the pre-emergence application comprises seed treatment. In a preferred embodiment, the fungicidal component is applied directly to the foliage (or leaves) of the plant.

The compositions according to the invention are suitable for treating a wide range of plants. Crop plants that can be treated using the present invention include: cereals (e.g., wheat, barley, rye, oats, corn, rice, sorghum, triticale, and related crops); beets, such as sugar beets and fodder beets; fruits such as pomes, stone fruits, and soft fruits (e.g., apples, pears, grapes, plums, peaches, almonds, cherries, and berries (e.g., strawberries, raspberries, and blackberries)); legumes, for example, kidney beans, lentils, peas, soybeans; oil plants, such as rape, mustard, sunflower; melons, for example, the melons cantaloupe (chieh-qua), cucumber, cantaloupe; fiber plants, such as cotton, flax, hemp and jute; citrus fruits such as oranges, lemons, grapefruits and mandarins; vegetables, such as spinach, lettuce, asparagus, cabbage, carrot, onion, tomato, potato, pepper; coffee; and ornamental plants, such as flowers, shrubs, broad-leaved trees or evergreens (e.g., conifers).

The invention is particularly suitable for the treatment and protection of cereals, fruits, legumes, oil plants, lemons and vegetables, in particular soybeans, cotton, dried beans, sugar cane, tomatoes, potatoes and rice.

The compositions, methods and uses of the invention are useful for preventing, controlling or treating infections caused by a range of fungal pathogens. Fungal infections that may be treated using the present invention include, but are not limited to: alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), Aureobasidium spp.), Bipolaris spp (Bipolar spp.), Black spp, Blumeria (Blumeria spp.), Botrytis spp, Peronospora (Bremia spp.), Cercospora (Cercospora spp.), Cercospora spp (Cercospora spp.), Cladospora spp, Cladosporium spp, Cochlospora spp, Colletotrichia spp, Diynia spp (Corespora spp.), Microsporum spp, Sporidiobolus (Hypocrea spp.), Erysiporium spp), Lecospora spp (Marcospora spp.), Leptosporium spp., Leptosporium spp, Ercospora spp (Leptosporidium spp.), Erysia (Corespora spp.), Erysiphe spp.), Leptosporium spp Mycosphaerella sp., Peronospora spp, Sphaerotheca sp., Phaeosphaera sp., Phellinus sp., Phoma sp., Phytophthora sp., Sphaerotheca sp., Phytophthora sp., Plasmopara sp., Pseudoperonospora sp., Pyrenophora sp., Phytophthora sp., Pyrenophora sp., Pyrenophora.

The present invention is particularly effective in preventing or treating infections caused by the following species: alternaria (Alternaria spp.), ascospora (Ascochyta spp.), Cercospora (Cercospora spp.), Cochlospora (Cochlobolus spp.), Colletotrichum (Colletotrichum spp.), Alternaria (Colletotrichum spp.), Septoria (Didymella spp.), Erysipelothrix (Erysiphe spp.), Mycosphaerella (Mycosphaerella spp.), Peronospora (Peronospora spp.), Hymenoxenia (Phakopsora spp.), Phytophora (Phytophora spp.), Rhizoctonia (Rhizoctonia spp.), Sclerotia (Sclerotinia spp.), and Septoria (Septoria spp.).

Fungal pathogens which can be controlled using the present invention are, for example, Alternaria cucumerina (Alternaria cuprina) (blight), Alternaria carotovora (Alternaria dauci) (leaf spot), Alternaria solani (Alternaria porifera) (purple leaf spot), Alternaria solani (Alternaria solani) (early blight), Alternaria (Alternaria spp.) (blight), Alternaria alternata (Alternaria spp.) (leaf spot), Alternaria alternata (Alternaria spp.) (paniculate spot), Alternaria alternata (Alternaria spp.) (leaf spot and Alternaria spongiosa), Alternaria sporogenes (cochyta) (blight), Alternaria sporulata (Alternaria sporulata), Alternaria sporulata (Alternaria sporulata) (leaf spot and Alternaria sporulata) (Alternaria sporulata), Alternaria sporulata (Alternaria sporulata) (maize blight), Alternaria sporulata (Alternaria sporulata) (maize sporulata), Alternaria sporulata (maize sporulata), Alternaria sporulata (maize sporulata), maize sporulation, maize, Wheat powdery mildew (Blumeria graminis), Botrytis cinerea (Botrytis cinerea) (Botrytis Botrytis gray mold), Botrytis cinerea (Botrytis cinerea) (Botrytis gray mold), Botrytis cinerea (Botrytis cinerea) (Gray rot), Botrytis spp (Gray rot), Bremia lactucae (downy mildew), Pseudocercospora arachidicola (Cercospora arachidicola) (early leaf spot), Cercospora (Cercospora) (blight and leaf spot), Cercospora kikuchi (Cercospora kikuchi) (Cercospora blight and leaf spot), Cercospora purpurea (Cercospora purpurea) (leaf spot disease), Pseudocercospora sojnina) (eye disease, leaf spot disease), Cercospora sorghoniensis (Cercospora sojae) (leaf spot disease), Cercospora sporum (Cercospora sporum) (leaf spot disease), Gray leaf spot disease (Cladospora sporum) (leaf spot disease), Gray spot disease (Cladospora sporum (Cladosporium), Gray spot disease (Gray spot disease), Gray leaf spot disease (Cladospora sporum (Cladosporium), Gray spot disease (Cladosporum) of corn sporum (Cladospora (Cladosporium), Gray spot disease (Cladospora sporum (Cladosporum) of corn sporum (Gray spot disease (Cladosporum) of corn (Cladospora) of corn (Gray spot disease), Gray spot disease (Gray spot disease), Gray spot disease, leaf spot disease, Gray spot disease, leaf spot disease, Gray spot disease, leaf spot disease, Gray spot disease, leaf spot disease, Gray spot disease, leaf spot disease, Gray spot disease, leaf spot disease, Gray spot disease, leaf spot disease, Gray spot disease, examples of the disease include, but are not limited to, Cochliobolus graminis (Cochliobolus sativus) (cyanosis), Cochliobolus heterosporum (Cochliobolus heterosporum) (southern corn leaf blight), Colletotrichum graminearum (Colletotrichum graminicola) (anthracnose, leaf blight and stalk rot), Colletotrichum (Colletotrichum spp.) (anthracnose), Colletotrichum truncatum (Colletotrichum truncatum) (anthracnose), Corynespora polyspora (corynesicola) (target spot), sphaceliopsis phaseoloides (Diaporthe phaseoloum) (pod and stalk blight), sphaera citrullaria (dimorpha bryoniae) (diaphora sphaerothecoides), sphaera leucotrichum (sphaera) and sphaera (sphaerothecium blight), sphaera graminis (erysipelas graminis) (powdery mildew), sphaera (Erysiphe sphaera) and sphaera (sphaera) blight (sphaera), sphaera (sphaera) sphaera (sphaera) and sphaera (sphaera) blight (sphaera) and sphaera (sphaera) of the species (sphaera) of sphaera (sphaera) and (sphaera) of the species (sphaera) of sphaera (sphaera) of sphaeria) of the species (sphaeria) of the plant (sphaeria) of the species (sphaeria) of the plant (sphaeria) of the plant (sphaeria) of the plant, sphaeria) sphaera (sphaeria) of the plant (sphaera (sphaeria) sphaeria dotella (sphaeria) of the species (sphaeria) sphaeria, sphaeria) of (sphaeria) sphaeria, sphaeria (sphaeria) of (sphaeria) of (sphaeria) sphaeria (sphaeria ) sphaeria (sphaeria) sphaeria, sphaeria) sphaeria, sphaeria (sphaeria) sphaeria (sphaeria, sphaeria (sphaeria, sphaeria (sphaeria) sphaeria (sphaeria) sphaeria (sphaeria) sphaeria (sphaeria) sphaeria, Mycosphaeria nodorum (Leptosphaeria nodorum) (septoria nodorum), Lactuca sativa (Marssonia pantoea) (anthracnose), Mycosphaera graminicola (Mycosphaera graminicola), Mycosphaera spp (Mycosphaera spp.) (Mycosphaera blight), Peronospora arborescens (Peronospora arborescens) (downy mildew), Peronospora destructor (Peronospora destructor) (downy mildew), Peronospora sojae (Peronospora manshurica) (downy mildew), Sphaerotheca nodorum (Phaospora nodorum), Phytophthora sp.phakopsorales (Phakopsora sporum), Phytophthora sp.sp.sp.sp.cruentosa (Phoma), Phytophthora sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.arachidis (Phytophthora nodorum), Phytophthora sp.sp.sp.sp.sp.sp.zeae (Phoma) and Phoma nigella (Phosporum) and Phoma nigella maydis (Phoma) origin, Phoma nigella sporum (Phoma) and Phosporum (Phosporum) Bulina var.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp.sp., Phytophthora infestans (Phytophthora infestans) (late blight), Phytophthora nicotianae (Phytophthora nicotiana) (downy mildew), Plasmopara viticola (Plasmopara viticola) (downy mildew), Pseudoperonospora cubensis (downy mildew), Pseudocercospora medicaginosa (Pseudocercospora medicinalis) (leaf spot), Puccinia graminis (Puccinia coronaria) (crown rust disease), Puccinia graminis (Puccinia hortorhei) (brown rust), Pseudocercospora zeae (Puccinia polyspora) (rust disease), Puccinia sorghi (Puccinia sorghi) (rust disease), Puccinia sorghii (Puccinia sorghi) (rust disease), Puccinia striiformidis (Puccinia striiformidis) (yellow rust disease), Puccinia triticcinia terricola (rust disease), Pyrenophora niruri (Rhizoctonia neritic) (lumen of Rhizoctonia), Rhizoctonia solani (Rhizoctonia solani) (Rhizoctonia solani (Rhizoctonia) and Rhizoctonia solani (Rhizoctonia niponaria, Rhizoctonia) of Rhizoctonia, Rhizoctonia (Rhizoctonia) of wheat, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia nivora) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can), Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, Rhizoctonia (Rhizoctonia) can, rhinochlorospora (Rhynchosporium), Sclerotinia (Sclerotinia), Sclerotinia sclerotiorum (Sclerotinia minor) (wilt disease of lettuce), Sclerotinia sclerotiorum (Sclerotinia minor) (wilt disease of Sclerotinia sclerotiorum), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum) (stalk rot disease of Sclerotinia sclerotiorum), Sclerotinia sclerotiorum (white mold), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum) (rot disease of Sclerotinia sclerotiorum, white mold), Sclerotinia sclerotiorum (rot disease and wilt disease), Septoria soybean Septoria (Septoria) (Septoria leaf spot), Septoria Septoria (Septoria Septoria), Septoria (Septoria), Septoria (Septoria), Septoria, chitin (Stagonospora spp.) (leaf spot and glume blight), Rhizoctonia botrys (Uncinula necator) (powdery mildew), and Phomonadaceae (Uromyces spp.) (rust).

The present invention is particularly effective in the treatment and control of the following species: alternaria spp, Septoria spp, Sphaerotheca spp, Botrytis cinerea, Brown spot of peanut (Cercospora arachidicola), Ashbya glycines (Cercospora sojina), Gray corn (Cercospora zeae-maydis), Sphaerotheca graminis (Cochliobolus sativus), Anthrax (Colletotrichum spp), Erysipellus (Erysiphe spp), Peronospora arborescens (Peronospora arborescens), Sphaerotheca (Phakosporum spp), Phytophthora infestans (Phytophthora infestans), Plasmopara viticola (Plasmopora virticus), Rhizoctonia solani (Rhizoctonia spp), Sclerotia infestans (Sclerotiella sclerotiorum), Sphaerotheca (Sclerotium tritici), and Hypocrea (Vitis vinifera necator).

The invention is advantageous when used for controlling plants against a range of fungal diseases, in particular the following diseases: gray mold, downy mildew, late blight, early blight, powdery mildew, gray leaf spot, sclerotinia, rice blast, banded sclerotial blight, leaf rust, glume blight, leaf brown spot, net blotch, brown stain, root rot, stalk rot, leaf rot and other rot diseases.

The invention will now be described by way of the following examples for illustrative purposes only.

Percentages are percentages by weight unless otherwise indicated.

Examples of the invention

Formulation examples

Wettable Powder (WP)

By mixing and grinding the active ingredients and adjuvants (5%

1494；8％

622S; kaolin (to 100g)) toWettable Powder (WP) formulations were prepared.

The composition of an exemplary wettable powder formulation is summarized below:

water dispersible granule (WG)

The water dispersible granule (WG) formulation was prepared as follows:

mixing the active ingredient and adjuvant (0.5%

(sodium lauryl sulfate, Witco Inc., Greenwich),

88B (sodium lignosulfonate, Weishi Vickers (Westvaco Corp)), potassium carbonate (make up to 100%)), mixed and ground, then wetted, extruded and dried to provide water dispersible granules.

The composition of an exemplary water dispersible granule formulation is summarized below:

suspending agent (SC)

The suspension formulation (SC) was prepared as follows:

the finely ground active ingredient was mixed with adjuvants (10% propylene glycol, 5% tristyrylphenol ethoxylate), 1% sodium lignosulfonate, 1% carboxymethylcellulose, 1% silicone oil (in the form of a 75% aqueous emulsion), 0.1% xanthan gum, 0.1% NIPACIDE BIT 20 and water (make up to 1L)).

The composition of exemplary aqueous suspension formulations is summarized below:

picoxystrobin	10％
		Boscalid	30％
Dimethomorph	20％
		Tristyrylphenol ethoxylate	5％
Lignosulfonic acid sodium salt	1％
		Carboxymethyl cellulose	1％
1% Silicone oil (in the form of a 75% aqueous emulsion)	1％
		Xanthan gum	0.1％
NIPACIDE BIT 20	0.1％
		Water (W)	To 1L

Soluble Granule (SG)

The Soluble Granule (SG) formulation was prepared as follows:

mixing the active ingredient with adjuvant (0.5%

(sodium lauryl sulfate, Witco Inc., Greenwich),

88B (sodium lignosulfonate, Weishiweck Corp) and 2% sodium bicarbonate (NaHCO)₃) Potassium sulfate (make up to 100%)) and ground and then wetted, extruded and dried to provide soluble granules.

The composition of an exemplary soluble granule formulation is summarized below:

a series of formulations were prepared using a series of different compositions as described above. The formulation types and active ingredient contents are summarized in the table below.

Examples 21-26 formulations are embodiments of the invention. The formulations of examples 1 to 20 are provided for comparative purposes.

The weight ratio of boscalid to dimethomorph to picoxystrobin in examples 21 to 26 was as follows:

example 21: 1:1.5:1.5

Example 22: 3:2:1

Example 23: 1.5:1:1

Example 24: 4:1:1

Example 25: 1.5:1:1

Example 26: 6:1:1.6

Biological examples

The expected activity of a given combination of two active compounds can be calculated according to the following "Colby (kolbe) formula" [ s.r.colby, Weeds [ Weeds ],15,20-22, (1967) ]:

E＝A+B-(A×B/100)

wherein:

efficacy of compound a used at a dose of m grams per hectare (g/ha) (%);

b ═ efficacy of compound B used at a dose of n grams per hectare (g/ha) (%);

e ═ the expected activity (%) of the combination given when compound a and compound B were used together at m grams per hectare (g/ha) and n grams per hectare (g/ha), respectively.

The expected activity of a given combination of three active compounds can be calculated according to the following formula:

E＝(A+B+C)-(A×B+A×C+B×C)/100+A×B×C/10000

wherein:

efficacy of compound a used at a dose of m grams per hectare (g/ha) (%);

b ═ efficacy of compound B used at a dose of n grams per hectare (g/ha) (%);

efficacy of compound C used at a dose of p grams per hectare (g/ha) (%);

e ═ the expected activity (%) of the combination given when compound a, compound B and compound C were used together at doses of m grams per hectare (g/ha), n grams per hectare (g/ha) and p grams per hectare (g/ha), respectively.

Field test 1: barley-Ciclosporium gramineum

Young barley plants were sprayed with a conidia suspension of c.graminicola and cultured at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

The plants were divided into groups and the formulations of examples 1 to 6 and 21 were used to treat infected plants. One group of plants was left untreated as a control group.

The treated plants were kept in a greenhouse at 15 ℃ and at 80% relative atmospheric humidity for 15 days. Thereafter, fungicidal efficacy was evaluated.

The results of the evaluation are listed in table a below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

TABLE A

The results listed in table a show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling barley infection caused by c.graminicola.

And (3) field test 2: phaseolus ascochyta

Young legumes were sprayed with a conidia suspension of the genus ascochyta and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

The plants were divided into groups and the formulations of examples 1 to 3, 7 to 9 and 22 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table B below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

TABLE B

The results listed in table B show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling legume infections caused by ascochyta.

And (3) field test: genus Raphanus-Erysiphe

Young radish plants were sprayed with a conidia suspension of erysiphe spp and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

Plants were divided into groups and the formulations of examples 1 to 3, 12 to 14 and 24 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table C below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

Watch C

The results listed in table C show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling radish infections caused by erysiphe.

And (4) field test: corn-corn gray spot pathogen

Young maize plants were sprayed with a conidia suspension of the species alternaria charantia and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

Plants were divided into groups and the formulations of examples 1 to 3, 10 to 12 and 23 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table D below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

Table D

The results presented in table D show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling corn plant infections caused by botrytis cinerea.

And (5) field test: melon Alternaria and anthrax

Young melon plants were sprayed with conidial suspensions of alternaria and anthrax, respectively, and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

Plants were divided into groups and the formulations of examples 1 to 3, 15 to 17 and 25 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table E below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

TABLE E

The results listed in table E show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling melon plant infections caused by alternaria and anthrax.

And (6) field test: vitis vinifera, Plasmopara viticola and Uncaria botrytis

Young grape plants were sprayed with conidia suspensions of botrytis, peronospora viticola and leptospirillum viticola, respectively, and cultured at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

The results of the evaluation are listed in table F below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

The results listed in table F show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling grape plant infection caused by alternaria and anthrax.

And (7) field test: rape-tree-like downy mildew

Young rape plants were sprayed with a conidia suspension of peronospora arborescens and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

Plants were divided into groups and the formulations of examples 1 to 3, 10 to 12 and 26 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table G below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

Watch G

The results listed in table G show that the combination of boscalid, dimethomorph and picoxystrobin shows a synergistic level of activity in controlling infections of rape plants caused by peronospora arborescens.

And (4) field test 8: peanut-peanut brown spot pathogen

Young peanut plants were sprayed with a conidia suspension of peanut brown spot pathogen and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

Plants were divided into groups and the formulations of examples 1 to 3, 7 to 9 and 22 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table H below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

Watch H

The results listed in table H show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling peanut plant infection caused by peanut brown spot pathogen.

And (3) field test 9: pisum sativum-sclerotinia sclerotiorum

Young pea plants were sprayed with a conidia suspension of sclerotinia sclerotiorum and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

The results of the evaluation are listed in table I below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

TABLE I

The results set out in table I show that the combination of boscalid, dimethomorph and picoxystrobin shows a synergistic level of activity in controlling pea plant infections caused by sclerotinia sclerotiorum.

Field test 10: phytophthora infestans in potato

Young potato plants were sprayed with a conidia suspension of phytophthora infestans and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

Plants were divided into groups and the formulations of examples 1 to 3, 18 to 20 and 26 were used to treat infected plants. One group of plants was left untreated as a control group.

The results of the evaluation are listed in table J below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

TABLE J

The results listed in table J indicate that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling potato plant infection caused by phytophthora infestans.

And (3) field test 11: soybean-soybean Gray leaf mold, Phyllosora and Rhizoctonia solani

Young soybean plants were sprayed with conidia suspensions of soybean griseofulvin, phakopsora and rhizoctonia solani, respectively, and cultured at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

The results of the evaluation are listed in table K below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

The results listed in table K show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling soybean plant infections caused by botrytis cinerea, phakopsora and rhizoctonia solani.

And (3) field test 12: wheat-septoria tritici

Young wheat plants were sprayed with a conidia suspension of septoria tritici and incubated at 20 ℃ and 100% relative atmospheric humidity for 48 hours.

The results of the evaluation are listed in table L below. 100% means that no fungal infection was observed and 0% corresponds to the efficacy of the control group.

Watch L

The results listed in table L show that the combination of boscalid, dimethomorph and picoxystrobin showed a synergistic level of activity in controlling wheat plant infection caused by septoria tritici.

As will be appreciated by those skilled in the art, the foregoing description is only exemplary and descriptive of some specific examples and is not intended to limit the scope of the invention, particularly the scope of the claims. The scope of the invention is determined by the appended claims.

Claims

1. A fungicidal composition comprising boscalid, dimethomorph and picoxystrobin, wherein the composition comprises from 10 to 60% by weight boscalid, from 5 to 45% by weight dimethomorph and from 5 to 40% by weight picoxystrobin.

2. The fungicidal composition according to claim 1, wherein boscalid is present in an amount of from 10 to 45% by weight.

3. The fungicidal composition according to any one of claims 1-2, wherein boscalid, dimethomorph and picoxystrobin are present in a weight ratio of boscalid from 0.5 to 8.0, dimethomorph from 0.5 to 3.0, picoxystrobin from 0.5 to 3.0.

4. The fungicidal composition according to claim 3, wherein boscalid, dimethomorph and picoxystrobin are present in a weight ratio of boscalid from 0.8 to 7.0, dimethomorph from 0.8 to 2.2, picoxystrobin from 0.8 to 2.0.

5. The fungicidal composition according to any one of claims 1-2, wherein the weight ratio of boscalid to the combined amount of dimethomorph and picoxystrobin is from 1:100 to 100: 1.

6. The fungicidal composition according to claim 5, wherein the weight ratio of boscalid to the combined amount of dimethomorph and picoxystrobin is from 1:25 to 25: 1.

7. The fungicidal composition according to claim 6, wherein the weight ratio of boscalid to the combined amount of dimethomorph and picoxystrobin is from 1:10 to 10: 1.

8. The fungicidal composition according to any one of claims 1-2, further comprising one or more adjuvants selected from: one or more extenders, carriers, solvents, surfactants, stabilizers, defoamers, antifreeze agents, preservatives, antioxidants, colorants, thickeners, solid adhesion agents, and inert fillers.

9. The fungicidal composition according to any one of claims 1 to 2, wherein the composition is a soluble Solution (SL), an Emulsifiable Concentrate (EC), an aqueous Emulsion (EW), a Microemulsion (ME), a Suspension (SC), an oil suspension (OD), a suspended seed coating (FS), a water dispersible granule (WG), a Soluble Granule (SG), a Wettable Powder (WP), a Soluble Powder (SP), a Granule (GR), a micro-Capsule Granule (CG), a Fine Granule (FG), a macro-granule (GG), an aqueous Suspoemulsion (SE), a micro-Capsule Suspension (CS), or a micro-granule (MG).

10. The fungicidal composition according to claim 9, wherein the composition is a water dispersible granule (WG), a suspending agent (SC), a Wettable Powder (WP), or a Soluble Granule (SG).

11. A method for preventing, treating and/or controlling a fungal infection in a target plant, the method comprising applying to the plant, part of the plant or surroundings thereof a composition comprising boscalid, dimethomorph and picoxystrobin, wherein the composition comprises from 10 to 60% by weight boscalid, from 5 to 45% by weight dimethomorph and from 5 to 40% by weight picoxystrobin.

12. The method according to claim 11, wherein the rate of application of the total amount of boscalid, dimethomorph and picoxystrobin is in the range of from 10 grams per hectare (g/ha) to 9000 g/ha.

13. The method according to claim 12, wherein the application rate of the total amount boscalid, dimethomorph and picoxystrobin is from 20g/ha to 5000 g/ha.

14. The method according to any one of claims 11 to 13, wherein the rate of boscalid application is from 10g/ha to 2000 g/ha.

15. The method according to any one of claims 11 to 13, wherein the rate of application of dimethomorph is from 10g/ha to 800 g/ha.

16. The method according to any one of claims 11 to 13, wherein picoxystrobin is applied at a rate of from 10 to 800 g/ha.

17. The method according to any one of claims 11 to 13, wherein boscalid, dimethomorph and picoxystrobin are applied simultaneously.

18. The method according to any one of claims 11 to 13, wherein boscalid, dimethomorph and picoxystrobin are applied sequentially.

19. The method according to any one of claims 11 to 13, wherein boscalid, dimethomorph and picoxystrobin are each applied a plurality of times.

20. The method according to any one of claims 11 to 13, wherein boscalid, dimethomorph and picoxystrobin are applied to the foliage of the target plants.

21. A method according to any one of claims 11 to 13, wherein the plants treated comprise cereals, sugar beets, fruits, oil plants, melons, fibre plants, citrus fruits, vegetables, coffee and ornamental plants.

22. The method according to claim 21, wherein the target plants are selected from the group consisting of grains, fruits, legumes, oil plants, lemons, and vegetables.

23. The method according to any one of claims 11 to 13, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of infections caused by: alternaria sp, Septoria sp, Aureobasidium sp, Diphyllum sp, Blackspot sp, Bluezia sp, Bluemeria sp, Botrytis sp, Bluenia sp, Cercospora sp, Cladosporium sp, Cochiulospora sp, Colletotrichum sp, Dicospora sp, Corcospora sp, Spirosporum sp, Spherosporium sp, Leptosporium sp, Erysiporium sp, Erysiphe sp, Marcospora sp, Leptosporium sp, Marcospora sp, Helicosporium sp, and Helicosporium sp Mycosphaerella sp., Peronospora spp, Sphaerotheca sp., Phaeosphaera sp., Phellinus sp., Phoma sp., Phytophthora sp., Sphaerotheca sp., Phytophthora sp., Plasmopara sp., Pseudoperonospora sp., Pyrenophora sp., Phytophthora sp., Pyrenophora sp., Pyrenophora.

24. The method of claim 23, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of infections caused by: alternaria, Septoria, Cercospora, Sporotrichum species, anthrax, Aschersonia, Erysiphe, Mycosphaerella, Peronospora, Hymenochaete, Phytophthora, Rhizoctonia, Sclerotinia, and Septoria.

25. The method of claim 23, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of infections caused by: alternaria, Septoria, Botrytis cinerea (Botrytis cinerea), peanut brown spot pathogen (Cercospora arachidicola), soybean gray spot pathogen (Cercospora sojina), corn gray spot pathogen (Cercospora zeae-maydis), Cochliobolus graminis (Cochliobolus sativus), Anthragma (Colletotrichum spp.), Erysiphe (Erysiphe spp.), Peronospora arborescens (Peronospora arborescens), Hymenochaete, Phytophthora infestans (Phytophthora infestans), Plasmopara viticola (Plasmopara viticola), Rhizoctonia solani (Rhizoctonia solani), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Sphaerothecium tritici (Tortomium tritici), and Uncaria vinifera (hook).

26. The method of claim 23, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of: gray mold, downy mildew, late blight, early blight, powdery mildew, gray leaf spot, sclerotinia, rice blast, banded sclerotial blight, leaf rust, glume blight, leaf brown spot, net blotch, brown stain, root rot, stalk rot, leaf rot and other rot diseases.

27. Use of a composition comprising boscalid, dimethomorph and picoxystrobin for preventing, treating and/or controlling a fungal infection in a target plant, wherein the composition comprises from 10 to 60% by weight boscalid, from 5 to 45% by weight dimethomorph and from 5 to 40% by weight picoxystrobin.

28. The use according to claim 27, wherein boscalid, dimethomorph and picoxystrobin are used in a total amount of from 10 grams per hectare (g/ha) to 9000 g/ha.

29. Use according to claim 28, wherein boscalid, dimethomorph and picoxystrobin are used in a total amount of from 20g/ha to 5000 g/ha.

30. Use according to any one of claims 27 to 29, wherein boscalid is used in an amount of from 10g/ha to 2000 g/ha.

31. Use according to any one of claims 27 to 29, wherein dimethomorph is used in an amount of from 10g/ha to 800 g/ha.

32. Use according to any one of claims 27 to 29, wherein picoxystrobin is used in an amount of from 10g/ha to 800 g/ha.

33. Use according to any one of claims 27 to 29, wherein boscalid, dimethomorph and picoxystrobin are used simultaneously.

34. Use according to any one of claims 27 to 29, wherein boscalid, dimethomorph and picoxystrobin are used sequentially.

35. Use according to any one of claims 27 to 29, wherein boscalid, dimethomorph and picoxystrobin are each used a plurality of times.

36. Use according to any one of claims 27 to 29, wherein boscalid, dimethomorph and picoxystrobin are applied to the foliage of the target plants.

37. Use according to any one of claims 27 to 29, wherein the plants treated comprise cereals, sugar beets, fruits, oil plants, melons, fiber plants, citrus fruits, vegetables, coffee, and ornamental plants.

38. Use according to claim 37, wherein the target plants are selected from cereals, fruits, legumes, oil plants, lemons and vegetables.

39. Use according to any one of claims 27 to 29, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of infections caused by: alternaria, Septoria, Aureobasidium, Bismith, Bluella, Blumeria, Botrytis, Peronospora, Cercospora, Cladosporium, Coccidioides, anthrax, Corynospora, Aschersonia, Erysipelis, Erysipellicum, Podosporum, Helicoverruca, Helicoveromyces, Microsphaera, Calycota, Hyalochaetala, Hymenochaetala, Phoma, Fomitomyces, Chytridium, Phytophthora, Plasmopara, Pseudoperonospora, Pseudoverrucaria, Puccinia, Pyrenophora, Rhizoctonia, Sclerotia, Petasites, Sporotrichum, Sphingomonas, and Ruscus.

40. Use according to claim 39, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of infections caused by: alternaria, Septoria, Cercospora, Sporotrichum, anthrax, Aschersonia, Erysiphe, Mycosphaerella, Peronospora, Hyriopsis, Phytophthora, Rhizoctonia, Sclerotinia, and Septoria.

41. Use according to claim 39, wherein the fungal infection prevented, treated and/or controlled is selected from the group consisting of infections caused by: alternaria, Septoria, Botrytis, peanut brown spot, soybean gray spot, corn gray spot, Cocospora graminicola, Colletotrichum, Blumeria, Peronospora, Blastomyces, Pseudoperonospora arborescens, Phytophthora infestans, Plasmopara viticola, Rhizoctonia solani, Sclerotinia sclerotiorum, Septoria tritici, and Uncaria botrys.

42. Use according to claim 39, wherein the fungal infection prevented, treated and/or controlled is selected from the following: gray mold, downy mildew, late blight, early blight, powdery mildew, gray leaf spot, sclerotinia, rice blast, banded sclerotial blight, leaf rust, glume blight, leaf brown spot, net blotch, brown stain, root rot, stalk rot, leaf rot and other rot diseases.