CN107582430A - A kind of methods for making and using same of highland barley beta glucan poly-aspartate compound - Google Patents
A kind of methods for making and using same of highland barley beta glucan poly-aspartate compound Download PDFInfo
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- CN107582430A CN107582430A CN201711054169.5A CN201711054169A CN107582430A CN 107582430 A CN107582430 A CN 107582430A CN 201711054169 A CN201711054169 A CN 201711054169A CN 107582430 A CN107582430 A CN 107582430A
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- highland barley
- poly
- aspartate
- barley beta
- dextran
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Abstract
The invention discloses a kind of methods for making and using same of highland barley beta glucan poly-aspartate compound, its preparation method is：Highland barley beta glucan, poly-aspartate be fully swelled with water, dissolve after, ethylene glycol or glycerine are added in 80 DEG C~100 DEG C reaction a period of times, reaction solution is freeze-dried, by freeze-dried powder put 55 DEG C~85 DEG C, relative humidity be 65%~85% and saturation KBr or saturated solution of potassium iodide environment in 10~20d of placing response, obtain highland barley beta glucan poly-aspartate compound.The compound can be applied in cosmetic humectant field, biologic adhesion preparation class drug field.The advantage of the invention is that：The compound has good performance of keeping humidity and bioadhesion performance.
Description
Technical field
The present invention relates to biomedicine technical field, more particularly to a kind of highland barley Beta-dextran-poly-aspartate compound
Methods for making and using same.
Background technology
Highland barley Beta-dextran is the family macromolecule SNSP for extracting to obtain from highland barley, and its chemical constitution is grape
The polymer that sugar unit is formed by β-(1 → 3) and β-(1 → 4) glycosidic bond.Highland barley Beta-dextran has certain water imbibition and viscoelastic
Property, therefore have potential use in cosmetic humectant and bioadhesive pharmaceutical preparation.
Poly-aspartate is a kind of polyaminoacid, and its structure is the polymer that aspartic acid units are formed by peptide bond.It is poly-
Aspartic acid has compared with strong absorptive, is commonly used for synthesizing high-hydroscopicity resin, used also as cosmetic humectant.
Compound is made by appropriate reaction in highland barley Beta-dextran and poly-aspartate, highland barley Beta-dextran can be improved
Some performances, so as to expand its application field, but this area still lacks highland barley Beta-dextran-poly-aspartate compound at present
Methods for making and using same.
The content of the invention
A kind of the defects of present invention is directed to prior art, there is provided the system of highland barley Beta-dextran-poly-aspartate compound
Standby and methods for using them, can effectively solve the problem that the above-mentioned problems of the prior art.
In order to realize above goal of the invention, the technical scheme that the present invention takes is as follows：
A kind of preparation method of highland barley Beta-dextran-poly-aspartate compound, comprises the following steps：
Step 1, by shown weight ratio scope, take 5~9 parts of highland barley Beta-dextran, 0.5~3 part of poly-aspartate；
Step 2, the water for adding parts by weight 100~200,10~15h is stirred, make fully swelling, dissolving；
Step 3, the ethylene glycol for adding highland barley Beta-dextran weight 5%~15%, control temperature as 80 DEG C~100 DEG C and protect
Constant temperature is held, stirs 4~8h, cooling；
Step 4, reaction solution is freeze-dried, obtains freeze-dried powder；
Step 5, freeze-dried powder is placed in constant-temperature enclosed container, controls temperature as 55 DEG C~85 DEG C and keep constant temperature, kept
Relative humidity is 65%~85%, and places saturation KBr or saturated solution of potassium iodide in container bottom, places 10~20d.
Preferably, the ethylene glycol described in step 3 is replaced with into glycerine.
The invention also discloses the application side of above-mentioned highland barley Beta-dextran-poly-aspartate compound, and it is applied to make up
The NMF of product, and as bioadhesive polymer, the pharmaceutic adjuvant applied to biologic adhesion preparation class medicine.
Compared with prior art the advantage of the invention is that：The performance of highland barley Beta-dextran is improved, is prepared for a kind of new
Compound (i.e. highland barley Beta-dextran-poly-aspartate compound), the complex biological adhesiveness is strong, moisture-retaining capacity is strong.
Embodiment
For the objects, technical solutions and advantages of the present invention are more clearly understood, by the following examples, the present invention is done into
One step describes in detail.
Embodiment 1
6 parts of highland barley Beta-dextran, 1 part of poly-aspartate are taken, adds 180 parts of water, 100rpm stirring 12h, adds 0.5 part the third three
Alcohol, 5h is stirred under the conditions of 90 DEG C, 100rpm, is then freeze-dried reaction solution, freeze-dried powder is placed in constant-temperature enclosed container,
75 DEG C of keeping temperature, relative humidity 80%, and saturation potassium bromide solution is placed in container bottom, constant temperature places 15d.
Embodiment 2：
8 parts of highland barley Beta-dextran, 1.5 parts of poly-aspartate are taken, adds 200 parts of water, 100rpm stirring 15h, adds 0.8 part of second
Glycol, 6h is stirred under the conditions of 80 DEG C, 100rpm, is then freeze-dried reaction solution, freeze-dried powder is placed in constant-temperature enclosed container
In, 65 DEG C of keeping temperature, relative humidity 70%, and saturation potassium bromide solution is placed in container bottom, constant temperature places 20d.
Embodiment 3：
6 parts of highland barley Beta-dextran, 1 part of poly-aspartate are taken, adds 150 parts of water, 100rpm stirring 11h, adds 0.9 part the third three
Alcohol, 6h is stirred under the conditions of 85 DEG C, 100rpm, is then freeze-dried reaction solution, freeze-dried powder is placed in constant-temperature enclosed container,
70 DEG C of keeping temperature, relative humidity 82%, and saturated solution of potassium iodide is placed in container bottom, constant temperature places 12d.
Embodiment 4：
A kind of moisturizing essence：Highland barley Beta-dextran-poly-aspartate compound 2.0g, D-pantothenyl aleohol 0.5g, aloe is taken to carry
Take thing 1.0g, licorice 1.0g, water-soluble leaves of pulse plants leaves of pulse plants fragrant plant oil 3.0g, L-Ascorbic Acid L-O-Phosphate 0.5g, 1,4- butanediol
5.0g, xanthans 0.1g, allantoin 0.2g, pungent glycol 2.0g, water 84.7g, are made Essence 100g.
Embodiment 5：
A kind of Bio-adhesive tablets：Take ginseng extract (or other Chinese medical extracts) 5.0g, highland barley Beta-dextran-poly-
Asparaginic acid complex 8.0g, polyvinylpyrrolidone 1.0g, microcrystalline cellulose 8.0g, lactose 8.0g, 100, tablet is made.
The investigation of reaction effect：
According to literature method (Kato A, Sasaki Y, Furuta R, et al.Functional protein-
polysaccharide conjugate prepared by controlled dry-heating of ovalbumin-
dextran mixtures.Agricultural and Biological Chemistry,1990,54(1):107-112.) comment
Covalent reaction effect, the absorbance of partial reaction of the present invention or the product reacted completely in 470nm is determined, and with glucose and poly- day
Absorbance of the reaction product of winter propylhomoserin in 470nm is compared, and is calculated as the following formula：
R=(Asample/AGlc) × 100%
In formula, R be the extent of reaction ratio (value is more big, reacts more complete), AsampleFor this programme partial reaction or
Complete reaction product is in 470nm absorbance, AGlcFor the reaction product of glucose and poly-aspartate 470nm absorbance.
1. glucose and the measure of poly-aspartate reaction product：Glucose 6.0g, poly-aspartate 1.0g are taken, adds water
180ml, 100rpm stir 12h, add 0.5g glycerine, 5h is stirred under the conditions of 90 DEG C, 100rpm, then freezes reaction solution
Dry, freeze-dried powder is placed in constant-temperature enclosed container, 75 DEG C of keeping temperature, relative humidity 80%, and place and satisfy in container bottom
And potassium bromide solution, constant temperature place 15d.Take reaction product to be made into the 10mg/ml aqueous solution, A is determined at 470nmGlc。
1. highland barley Beta-dextran reaction one (addition crosslinking agent glycerine)：Take highland barley Beta-dextran 6.0g, poly-aspartate
1.0g, add water 180ml, 100rpm stirring 12h, add 0.5g glycerine, 5h is stirred under the conditions of 90 DEG C, 100rpm, then will
Reaction solution is freeze-dried, and takes freeze-dried powder to be made into the 10mg/ml aqueous solution, and A is determined at 470nmsample, R values are calculated, as a result R=
23.7%.
2. highland barley Beta-dextran reacts two (dry heat treatments)：Highland barley Beta-dextran 6.0g, poly-aspartate 1.0g are taken, adds water
180ml, 100rpm stir 12h, are then freeze-dried, freeze-dried powder is placed in constant-temperature enclosed container, 75 DEG C of keeping temperature,
Relative humidity 80%, and saturation potassium bromide solution is placed in container bottom, constant temperature places 15d.Reaction product is taken to be made into 10mg/ml
The aqueous solution, at 470nm determine Asample.R values are calculated, as a result R=41.3%.
3. highland barley Beta-dextran reaction three：Highland barley Beta-dextran 6.0g, poly-aspartate 1.0g are taken, adds water 180ml,
100rpm stirs 12h, adds 0.5g glycerine, 5h is stirred under the conditions of 90 DEG C, 100rpm, is then freeze-dried reaction solution,
Freeze-dried powder is placed in constant-temperature enclosed container, 75 DEG C of keeping temperature, relative humidity 80%, and saturation bromination is placed in container bottom
Potassium solution, constant temperature place 15d.Take reaction product to be made into the 10mg/ml aqueous solution, A is determined at 470nmsample.R values are calculated,
As a result R=73.6%.
It is above-mentioned test result indicates that, the reaction of highland barley Beta-dextran and poly-aspartate, only add crosslinking agent glycerine, or
Dry heat treatment is only carried out, the extent of reaction is below the reaction not only added glycerine but also carry out dry heat treatment.
Experiment shows in addition, above-mentioned " in reaction three ", to change glycerine into ethylene glycol, KBr changes KI into, as a result
It is similar, R=71.9%.
The moisture retention of product is investigated：
(Gao Ruiying, Zhang Xiuyu, Mu Dan, the bioactive polysaccharide moisture absorption used for cosmetic such as hyaluronic acids is waited according to literature method
Performance of keeping humidity measure Guangdong chemical industry, 2009,36 (10):230-232.) determine highland barley Beta-dextran, poly-aspartate, highland barley β-
The moisture absorption of glucan-poly-aspartate compound and moisture-retaining capacity are as a result as follows.During the environment of relative humidity 81% placement 72h
Hydroscopicity：Highland barley Beta-dextran is 16.1%, poly-aspartate 22.4%, compound 34.6%；The ring of relative humidity 43%
Place hydroscopicity during 72h in border：Highland barley Beta-dextran is 6.6%, poly-aspartate 7.4%, compound 8.2%.Use silicon
Glue drier handles 72h moisturizing rate：Highland barley Beta-dextran is 58.7%, poly-aspartate 66.3%, and compound is
75.8%.
It is above-mentioned test result indicates that, the highland barley Beta-dextran-moisture absorption of poly-aspartate compound, moisture-retaining capacity are better than highland barley
Beta glucan and poly-aspartate.
The bioadhesive of product is investigated：
Adhesion is used as using highland barley Beta-dextran, poly-aspartate, highland barley Beta-dextran-poly-aspartate compound respectively
Agent, tablet is made by formula as below：Adhesive 6g, dextrin 12g, lactose 12g, are made 100 altogether.Not add matching somebody with somebody for adhesive
Side's (dextrin 15g, lactose 15g, 100 are made altogether) made tablet is as reference.According to literature method (Xiang great Xiong, Li Huande, sieve
Outstanding English, waits Pueraria Flavonid bioadhesive sustained release tablets vitro tissue adhesions and release research China Dispensaries, and 2002,13
(8):459-461.) determine bioadhesive, as a result, to the adhesion of isolated small intestines of rabbits tissue, be not added with the piece of adhesive
Agent is 0g/cm2, using the adhesion of tablet made of highland barley Beta-dextran as 5.3g/cm2, with tablet made of poly-aspartate
Adhesion be 6.8g/cm2, using the adhesion of highland barley Beta-dextran-tablet made of poly-aspartate compound as 10.6g/
cm2。
It is above-mentioned test result indicates that, the bioadhesive of highland barley Beta-dextran-poly-aspartate compound be better than highland barley β-
Glucan and poly-aspartate.
One of ordinary skill in the art will be appreciated that embodiment described here is to aid in reader and understands this hair
Bright implementation, it should be understood that protection scope of the present invention is not limited to such especially statement and embodiment.Ability
The those of ordinary skill in domain can be made according to these technical inspirations disclosed by the invention it is various do not depart from essence of the invention its
Its various specific deformations and combination, these deformations and combination are still within the scope of the present invention.
Claims (4)
1. the preparation method of a kind of highland barley Beta-dextran-poly-aspartate compound, it is characterised in that comprise the following steps：
Step 1, by shown weight ratio scope, take 5~9 parts of highland barley Beta-dextran, 0.5~3 part of poly-aspartate；
Step 2, the water for adding parts by weight 100~200,10~15h is stirred, make fully swelling, dissolving；
Step 3, the ethylene glycol for adding highland barley Beta-dextran weight 5%~15%, control temperature for 80 DEG C~100 DEG C and keep permanent
Temperature, stir 4~8h, cooling；
Step 4, reaction solution is freeze-dried, obtains freeze-dried powder；
Step 5, freeze-dried powder is placed in constant-temperature enclosed container, controls temperature as 55 DEG C~85 DEG C and keep constant temperature, keep relative
Humidity is 65%~85%, and places saturation KBr or saturated solution of potassium iodide in container bottom, places 10~20d.
2. a kind of preparation method of highland barley Beta-dextran-poly-aspartate compound according to claim 1, its feature
：Ethylene glycol described in step 3 is replaced with into glycerine.
3. a kind of preparation method of highland barley Beta-dextran-poly-aspartate compound according to claim 1 or 2, it is special
Sign is：The compound is applied to the NMF of cosmetics.
4. a kind of preparation method of highland barley Beta-dextran-poly-aspartate compound according to claim 1 or 2, it is special
Sign is：The compound is as bioadhesive polymer, the pharmaceutic adjuvant applied to biologic adhesion preparation class medicine.
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| CN201711054169.5A CN107582430B (en) | 2017-10-31 | 2017-10-31 | Preparation and application method of highland barley beta-glucan-polyaspartic acid compound |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109260094A (en) * | 2018-11-20 | 2019-01-25 | 上海悦目化妆品有限公司 | A kind of composition and preparation method thereof containing plant antiallergic ingredient |
| CN109431860A (en) * | 2018-12-28 | 2019-03-08 | 北京焉支山科技有限公司 | Cosmetics-stage poly (sodium aspartate) and daily chemical product containing the ingredient |
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| JPS6281372A (en) * | 1985-10-04 | 1987-04-14 | Terumo Corp | Unsaturated fatty acid amide derivative and blood platelet agglutination inhibitor containing said derivative |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS6281372A (en) * | 1985-10-04 | 1987-04-14 | Terumo Corp | Unsaturated fatty acid amide derivative and blood platelet agglutination inhibitor containing said derivative |
| US20140236082A1 (en) * | 2002-03-15 | 2014-08-21 | Abbott Cardiovascular Systems Inc. | Biocompatible Carrier Containing A Bioadhesive Material |
| CN103463453A (en) * | 2013-09-04 | 2013-12-25 | 贵州仙草生物科技有限公司 | Dendrobium stem tablets and extraction and preparation methods thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109260094A (en) * | 2018-11-20 | 2019-01-25 | 上海悦目化妆品有限公司 | A kind of composition and preparation method thereof containing plant antiallergic ingredient |
| CN109431860A (en) * | 2018-12-28 | 2019-03-08 | 北京焉支山科技有限公司 | Cosmetics-stage poly (sodium aspartate) and daily chemical product containing the ingredient |
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| CN107582430B (en) | 2020-10-27 |
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