CN107574119A - A kind of parallel filling device - Google Patents
A kind of parallel filling device Download PDFInfo
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- CN107574119A CN107574119A CN201710899613.7A CN201710899613A CN107574119A CN 107574119 A CN107574119 A CN 107574119A CN 201710899613 A CN201710899613 A CN 201710899613A CN 107574119 A CN107574119 A CN 107574119A
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- feed liquid
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- drain pipe
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Abstract
The invention discloses a kind of parallel filling device, for inputting feed liquid in each reactor to parallel reaction, including：Tank body, for taking up the feed liquid；Rabbling mechanism, acting on feed liquid in the tank body makes it well mixed；Cover, can cover makes its closed on the tank body；Drain pipe, one end extend to the bottom of the tank body, and the other end is divided into several drain pipe branch roads, and each drain pipe branch road is in a reactor；Pump for giving-out, being arranged on drain pipe branch road makes feed liquid enter the reactor via the discharge nozzle.Both from same tank body, the feed liquid in the tank body is uniformly mixed the feed liquid inputted for each reactor of parallel reactor by the stirring of rabbling mechanism.Some branched pipes that drain pipe extends are respectively arranged with Pump for giving-out to suction out feed liquid, and can regulate and control the flow that feed liquid flows through.The consistent of the amount of initial feed liquid in each reactor and quality is so achieved, lifts the confidence level of final result of study.
Description
Technical field
The present invention relates to parallel reactor technical field, specially a kind of parallel filling device.
Background technology
Due to the characteristic of microorganism, its incubation was both influenceed by external environmental condition, also interior in heredity by it
The influence of characteristic, its incubation can not repeat completely.Therefore, in its culture process optimization process, using parallel culture
Method, by eliminating system deviation, comparativity and the higher result of study of confidence level can be obtained.
In incubation, the influence of the inoculation situation of strain to follow-up cultivation result, except there is the difference of inoculum concentration, more
It is important that there is difference of the inoculation liquid in quality.Because the thalline in seed liquor, a generation can be bred per 20-40 minutes；
The mode that is inoculated with to small-sized fermentation tank generally use shaking flask simultaneously, now shaking flask enough dissolved oxygens can not be provided, can be right
Bacterium solution produces certain influence, and the time is longer, and influence is bigger.So that single reactor is inoculated with as an example, the several steps of seeded process point, when spending
Between in terms of 5 minutes, then for parallel reactor, each tank quantity reaches 4 even 8, takes time to reach 20-40 and divides
Clock, it is enough the quality discrepancy for causing strain liquid this period.The difference of the quality and quantity of seed liquor, can be from training to optimizing research scheme
Foster source just has an impact, that is, the condition of setting out there is inborn difference, can not avoid to the validity of result of study with can
Reliability produces interference.
The content of the invention
It is an object of the invention to provide the inoculation product of a kind of parallel filling device, as much as possible guarantee each tank of parallel reactor
Matter is all identical.
Technical scheme provided by the invention is as follows：A kind of parallel filling device, in each reactor to parallel reaction
Feed liquid is inputted, including：Tank body, for taking up the feed liquid；Rabbling mechanism, acting on feed liquid in the tank body makes it well mixed
And provide dissolved oxygen；Cover, can cover makes its closed on the tank body；Drain pipe, one end extend to the bottom of the tank body,
The other end is divided into several drain pipe branch roads, and each drain pipe branch road is in a reactor；Pump for giving-out,
Being arranged on drain pipe branch road makes feed liquid enter the reactor via the discharge nozzle.
The feed liquid inputted for each reactor of parallel reactor is both from same tank body, and the feed liquid in the tank body is by stirring
The stirring for mixing mechanism is uniformly mixed.Some branched pipes that drain pipe extends are respectively arranged with Pump for giving-out to suction out feed liquid,
And the flow that feed liquid flows through can be regulated and controled, the consistent of the amount of initial feed liquid in each reactor and quality is so achieved, especially
It is that the liquid quality and quantity for the initial seed that can cause each reactor when for being inoculated with is consistent, lifts final result of study
Confidence level.
Preferably, the parallel filling device also includes feed supplement pipe, and feed supplement pump is provided with the liquid supplementation pipe.Can be to tank body
Middle supplement feed liquid.
Specifically, the Pump for giving-out and the feed supplement pump can require according to the required accuracy, from peristaltic pump or syringe pump etc..
Preferably, the parallel filling device also includes the pinch valve being arranged on drain pipe branch road.
Specifically, the drain pipe branch road is provided with sebific duct or contact pin with the side that the reactor is connected.
Preferably, the parallel filling device also includes filtrated air air inlet and the tank pressure regulation dress for acting on the tank body
Put, temperature-adjusting device and pH adjusting meanss.
A kind of parallel filling device provided by the invention, can bring following at least one beneficial effect：
1st, both from same tank body, the feed liquid in the tank body passes through the feed liquid inputted for each reactor of parallel reactor
The stirring of rabbling mechanism is uniformly mixed.Some branched pipes that drain pipe extends are respectively arranged with Pump for giving-out to suction out material
Liquid, and the flow that feed liquid flows through can be regulated and controled, the consistent of the amount of initial feed liquid in each reactor and quality is so achieved,
It especially when for being inoculated with, can make it that the liquid quality and quantity of the initial seed of each reactor is consistent, lift final research knot
The confidence level of fruit.
2nd, to require basal medium it is consistent, using process adjustment as the parallel culture of comparative study method for, using
Method and apparatus, it can also eliminate moisture evaporation difference, training after carrying out sterilization quality because of the independent sterilization zone of each tank of parallel reactor, sterilize
Support in base and cause to disappear the difference of wild Oryza species concentration containing solid content etc. can be settled, and have culture medium and seed liquor characteristic concurrently
The component of mixed-culture medium and the difference of time-varying characteristics, realize the effect for eliminating condition deviation of setting out.
3rd, the tank body part of the present apparatus can increase culture medium it is in situ prepare, sterilizing, inoculation, culture, feed supplement, and ventilation with
Stirring carries out the cellar culture required function such as supplying oxygen, to meet to the parallel packing culture medium of each parallel reactor, parallel inoculation, this
Outside, parallel filling device of the invention applies also for the training mode that links, and the training mode is based on single tank culture a to timing
Between nutrient solution be dispensed into a kind of parallel research method that compares of culture of the more strategies for carrying out cultivating midway in parallel reactor.
Brief description of the drawings
Below by a manner of clearly understandable, preferred embodiment is described with reference to the drawings, to the above-mentioned of parallel filling device
Characteristic, technical characteristic, advantage and its implementation are further described.
Fig. 1 is the structural representation of parallel filling device.
Drawing reference numeral explanation：100th, tank body, 110, cover, 200, rabbling mechanism, 300, drain pipe, 310, Pump for giving-out,
400th, feed supplement pipe, 410, feed supplement pump, 500, control system, 510, pH electrodes, 511, pH regulation pipelines, 512, charging peristaltic pump,
520th, temperature electrode, 521, temperature-controlling system, 530, DO electrodes, 600, air inlet pipeline, 610, gas exhaust piping
Embodiment
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, control is illustrated below
The embodiment of the present invention.It should be evident that drawings in the following description are only some embodiments of the present invention, for
For those of ordinary skill in the art, on the premise of not paying creative work, other can also be obtained according to these accompanying drawings
Accompanying drawing, and obtain other embodiments.
To make simplified form, part related to the present invention is only schematically show in each figure, their not generations
Its practical structures as product of table.
Device embodiment one
As shown in figure 1, the present embodiment discloses a kind of parallel filling device, for being inputted in each reactor to parallel reaction
Feed liquid.Including：Tank body 100, rabbling mechanism 200, cover 110, drain pipe 300 and Pump for giving-out 310.
Tank body 100 is used to take up feed liquid, and cover 110 covers makes its closed on tank body 100.Rabbling mechanism 200, is acted on
Feed liquid is well mixed feed liquid in tank body 100, exemplary, as illustrated, rabbling mechanism 200 can be stirring for motor driven
Mix paddle structure.
Drain pipe 300, one end are divided into the branch road of several drain pipes 300, and each branch road of drain pipe 300 may be connected to one
In individual reactor, the side that particularly drain pipe 300 is connected with reactor is provided with sebific duct or contact pin, by the sebific duct or inserts
Pin is connected with reactor.And the other end extends to the bottom of tank body 100 to extract the feed liquid in tank body 100.Fluid reservoir goes out liquid
Pump 310, being arranged on the branch road of drain pipe 300 makes feed liquid enter reactor via discharge nozzle.Namely Pump for giving-out 310 it is dynamic
Under power is supported, the feed liquid in tank body 100 is directed to out via discharge nozzle 300, and then is able to by some branch roads of discharge nozzle 300
Shunting, and the flow in respective branch is controlled by each Pump for giving-out 310 simultaneously, the action can also pass through control by control system 500
It is identical to be adjusted into the amount of the feed liquid of each reactor to make several Pump for giving-out 310.
, can also be on the branch road of drain pipe 300 in addition to control load is coordinated by Pump for giving-out 310 and controlling organization
Pinch valve (being not drawn into figure) is set, by controlling the keying of pinch valve, and using tank pressure as power, to control dnockout amount.
Device embodiment two
On the basis of device embodiment one, as shown in figure 1, parallel filling device also includes feed supplement pipe 400, in liquid supplementation pipe
It is provided with feed supplement pump 410.Feed liquid can be inputted by feed supplement pipe 400 to add to tank body 100, exemplary, the He of Pump for giving-out 310
Feed supplement pump 410 can select peristaltic pump or syringe pump etc..
Device embodiment three
On the basis of device embodiment one and device embodiment two.Parallel filling device also includes acting on tank body
100 barometric control unit, temperature-adjusting device and pH adjusting meanss.
Exemplary, interface is provided with cover 110 to install temperature electrode 520, pH electrodes 510, DO electrodes 530,
Respectively to measure the temperature of feed liquid, pH value, dissolved oxygen and pressure in tank body, the registration of temperature electrode 520 is via control system point
The action of the final control temperature control mechanism 521 of analysis causes temperature is run according to default parameter to control, and pH electrodes 510 are via control
System, which is analyzed, finally controls the effect for feeding peristaltic pump 512 to control the reagent that pH regulation pipelines are passed through to adjust feed liquid in tank body
PH value.Air inlet pipeline 600 and gas exhaust piping 610 are also plugged on cover 110, regulate and control its intake and exhaust by control system, with dimension
Tank pressure needed for holding.Air inlet simultaneously maintains to supply oxygen by agitating device.
Namely cause the tank body 100 of parallel filling device to maintain normal condition of culture, it can so keep
The certain activity of seed liquor, and prevent parallel filling device microbiological contamination because of negative pressure.
Barometric control unit, temperature-adjusting device, air inlet and stirring apparatus of oxygen supply, and pH in this parallel filling device
The control units such as adjusting means and corresponding software can be both integrated in the switch board of parallel reactor, and it is flat can also to be integrated in this
In row filling device.
Embodiment of the method one
Based on more than device embodiment one, two or three in device, can into parallel reaction each reactor additive
Liquid, the system deviation that the feed liquid characteristic is changed over time and brought can be eliminated.The parallel culture kind of the microorganism that is particularly suitable for use in
The operation of sub- liquid inoculation.Its specifically used step is：
(1) inoculation container, various adapters and sebific duct thereon or contact pin are sterilized, after the completion of by the parallel dnockout
Each branch road of the drain pipe 300 of device is docked under aseptic conditions with the reactor feed mouth after each sterilizing respectively.
(2) while by seed liquor add in aseptic manner in tank body 100, start rabbling mechanism 200, in tank body 100
Seed liquor mixed.
(3) Pump for giving-out 310 on each branch road of drain pipe 300 is opened, seed liquor is suctioned out from tank body 100, with identical
Amount be synchronously assigned in each reactor.In addition it is power that can also be pressed by tank, and dnockout amount is controlled using pinch valve.
In addition, to requiring that basal medium unanimously, using process adjustment for the parallel culture of comparative study method, uses
This method and device, can also eliminate because the independent sterilization zone of each tank of parallel reactor come Volume Changes after sterilization quality, sterilizing it is different,
Caused to disappear the difference of wild Oryza species concentration in culture medium containing solid content can be settled, and have culture medium and seed liquor characteristic concurrently
The component of mixed-culture medium and the difference of time-varying characteristics, realize the effect for eliminating condition deviation of setting out.
Further, the tank body part of the present apparatus can also be designed and manufacture fully according to the pattern of reactor, bag
The preparation in situ of increase culture medium, sterilizing, inoculation are included, and is aided with the functions such as air agitation, temperature control, charging, is met to each parallel anti-
The parallel packing culture medium of device, and the pattern that can normally cultivate are answered, realizes that parallel inoculation and the pattern of parallel distribution nutrient solution are entered
Row linkage culture.
It should be noted that above-described embodiment can independent assortment as needed.Described above is only the preferred of the present invention
Embodiment, it is noted that for those skilled in the art, do not departing from the premise of the principle of the invention
Under, some improvements and modifications can also be made, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (6)
- A kind of 1. parallel filling device, for inputting feed liquid in each reactor to parallel reaction, it is characterised in that including：Tank body, for taking up the feed liquid,Rabbling mechanism, acting on feed liquid in the tank body makes it well mixed,Cover, can cover makes its closed on the tank body；Drain pipe, one end are drawn from the bottom of the tank body, and the other end is divided into several drain pipe branch roads, each drain pipe Branch road is in a reactor；Pump for giving-out, being arranged on drain pipe branch road makes feed liquid enter the reactor via the discharge nozzle.
- 2. parallel filling device according to claim 1, it is characterised in that：The parallel filling device also includes feed supplement Manage, feed supplement pump is provided with the liquid supplementation pipe.
- 3. parallel filling device according to claim 2, it is characterised in that：The Pump for giving-out and the feed supplement pump can select With peristaltic pump or syringe pump.
- 4. parallel filling device according to claim 1, it is characterised in that：The parallel filling device also includes being arranged on Pinch valve on drain pipe branch road.
- 5. parallel filling device according to claim 1, it is characterised in that：The drain pipe branch road connects with the reactor The side connect is provided with sebific duct or contact pin.
- 6. according to any described parallel filling devices of claim 1-5, it is characterised in that：The parallel filling device also includes Act on the barometric control unit, temperature-adjusting device, air inlet and stirring apparatus of oxygen supply, and pH adjusting meanss of the tank body Deng.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710899613.7A CN107574119A (en) | 2017-09-28 | 2017-09-28 | A kind of parallel filling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710899613.7A CN107574119A (en) | 2017-09-28 | 2017-09-28 | A kind of parallel filling device |
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| Publication Number | Publication Date |
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| CN107574119A true CN107574119A (en) | 2018-01-12 |
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| CN201710899613.7A Pending CN107574119A (en) | 2017-09-28 | 2017-09-28 | A kind of parallel filling device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08228757A (en) * | 1996-01-12 | 1996-09-10 | Hitachi Ltd | Method for supplying chemical solutiion for culture tank and apparatus therefor |
| CN102559783A (en) * | 2012-02-29 | 2012-07-11 | 浙江大学 | Method for producing butyric acid through fermentation of multilinked fiber bed bioreactor system |
| CN204490886U (en) * | 2015-02-13 | 2015-07-22 | 吉林大学 | A kind of biology cellulose fermentation system |
| CN204779387U (en) * | 2015-05-20 | 2015-11-18 | 天紫环保投资控股有限公司 | Fertile system of system with good oxygen stirring function of wall is scraped in rotation of heating cloth oxygen |
| CN205142965U (en) * | 2015-10-28 | 2016-04-13 | 江苏久禾生物科技发展有限公司 | Steam type mushroom growing room heating system |
| CN207512180U (en) * | 2017-09-28 | 2018-06-19 | 上海国强生化工程装备有限公司 | A kind of parallel filling device |
-
2017
- 2017-09-28 CN CN201710899613.7A patent/CN107574119A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08228757A (en) * | 1996-01-12 | 1996-09-10 | Hitachi Ltd | Method for supplying chemical solutiion for culture tank and apparatus therefor |
| CN102559783A (en) * | 2012-02-29 | 2012-07-11 | 浙江大学 | Method for producing butyric acid through fermentation of multilinked fiber bed bioreactor system |
| CN204490886U (en) * | 2015-02-13 | 2015-07-22 | 吉林大学 | A kind of biology cellulose fermentation system |
| CN204779387U (en) * | 2015-05-20 | 2015-11-18 | 天紫环保投资控股有限公司 | Fertile system of system with good oxygen stirring function of wall is scraped in rotation of heating cloth oxygen |
| CN205142965U (en) * | 2015-10-28 | 2016-04-13 | 江苏久禾生物科技发展有限公司 | Steam type mushroom growing room heating system |
| CN207512180U (en) * | 2017-09-28 | 2018-06-19 | 上海国强生化工程装备有限公司 | A kind of parallel filling device |
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