CN107549465A - A kind of green peel of walnut feed addictive and preparation method - Google Patents

A kind of green peel of walnut feed addictive and preparation method Download PDF

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Publication number
CN107549465A
CN107549465A CN201710611294.5A CN201710611294A CN107549465A CN 107549465 A CN107549465 A CN 107549465A CN 201710611294 A CN201710611294 A CN 201710611294A CN 107549465 A CN107549465 A CN 107549465A
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China
Prior art keywords
walnut
parts
green peel
feed addictive
preparation
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Pending
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CN201710611294.5A
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Chinese (zh)
Inventor
赖朋
曹旭
王成伟
马凡胤
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Xihua University
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Xihua University
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Priority to CN201710611294.5A priority Critical patent/CN107549465A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to Feed Manufacturing field, discloses a kind of green peel of walnut feed addictive and preparation method, and extracting green peel of walnut by a variety of extracting methods such as water extraction method, ethanol extraction method, methanol extraction is used as feed addictive.Antibiotic and antiviral drugs in the alternative feed of the present invention reduce its dosage, play and suppress domestic animals and birds common pathogen and virus breeding, regulation gut flora ratio, prophylaxis against infection diseases, the purpose for increasing survival rate.Application in this feed addictive includes poultry fodder and livestock feed.

Description

A kind of green peel of walnut feed addictive and preparation method
Technical field
The present invention relates to Feed Manufacturing field, more particularly to a kind of green peel of walnut feed addictive and preparation method.
Background technology
In the prior art, Modernize extensive animal husbandry feeding manner, while improving speed of production, also bring Many problems, livestock products quality reduces and various diseases, and largely addition antibioticses in feed easily occur for livestock and poultry Medicine and additive endanger to caused by human health, and these problems affect the sound development of China's animal husbandry.High-quality feed It is the guarantee for producing high-quality livestock products, China is there is an urgent need to improve the quality and quality of animal products at present, to animal health Grow it is beneficial, to the healthy and beneficial harmless feed of consumer.
The content of the invention
The present invention provides a kind of green peel of walnut feed addictive and preparation method, solves to be badly in need of production pair in the prior art Animal health growth is beneficial, to the technical problem of the healthy and beneficial harmless feed of consumer.
The purpose of the present invention is achieved through the following technical solutions:
A kind of green peel of walnut feed addictive, including walnut peel extract.
A kind of preparation method of green peel of walnut feed addictive, including following three kinds of methods:
Water extraction method:50g drying green peel of walnut is beaten into powder, 1000ml distilled water refluxing extraction 30min, in triplicate, mistake Filter, merging filtrate is concentrated under reduced pressure, to obtain water extract;
Ethanol extraction method:50g drying green peel of walnut is beaten into powder, 1000ml absolute ethyl alcohol refluxing extraction 30min, repeats three Secondary, filtering, merging filtrate is concentrated under reduced pressure, to obtain ethanol extract;
Methanol extraction:1000ml methanol is oozed into clear extraction 48h, filtering, merging filtrate is concentrated under reduced pressure, to obtain methanol Extract.
The present invention provides a kind of green peel of walnut feed addictive and preparation method, by water extraction method, ethanol extraction method, A variety of extracting methods such as methanol extraction extract green peel of walnut as feed addictive.Antibiosis in the alternative feed of the present invention Element and antiviral drugs reduce its dosage, play and suppress domestic animals and birds common pathogen and virus breeding, regulation enterobacteriaceae Group's ratio, prophylaxis against infection diseases, the purpose for increasing survival rate.Application in this feed addictive includes poultry fodder and poultry Use feed.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to embodiment The present invention is further detailed explanation.
The embodiments of the invention provide a kind of green peel of walnut feed addictive, including walnut peel extract.
Wherein, green peel of walnut refers mainly to outer (green grass or young crops) pericarp extract of walnut, and its source is all juglandaceae plant fruits Exocarp and middle pericarp.
In order to improve antibacterial characteristics, the present invention can also include:Desmodium 5-10 parts, rheum officinale 5-15 parts, radix scutellariae 5-15 Part, golden cypress 10-15 parts, dandelion 5-15 parts, Radix Isatidis 5-15 parts, Rhizoma Sparganii 10-15 parts, curcuma zedoary 5-15 parts, chrysanthemum indicum 5-15 Part, bighead atractylodes rhizome 5-15 parts, ginseng 10-15 parts.Using natural traditional Chinese medicine as primary raw material, formed through traditional handicraft finishing, advantage is The immunity of livestock and antiviral and antibacterial ability can be strengthened, while there is very significant anti-diarrhea effect, can be fast after use Speed is absorbed by organisms, and harm of the pathogen to body can be thoroughly removed after being combined with pathogen, controls epidemic situation rapidly, is made Cost is cheap, simple to operate, is adapted to large-scale production.
Contrast that common its ANTIMICROBIAL DATA of birds pathogenic bacteria is as shown in table 1, the influence to broiler chicken survival rate is to the such as institute of table 2 Show, contrast is influenceed on mouse intestinal flora quantity as shown in table 3, it is as follows:
Table 1
Note:Data are minimal inhibitory concentration (MIC) in table, unit:mg/ml
Table 2
Sample 20 ages in days 25 ages in days 30 ages in days 35 ages in days 40 ages in days
Water extract 97.2% 97.0% 96.8% 96.8%* 96.8%*
Ethanol extract 98.2% 98.0% 98.0%* 96.9%* 96.5%*
Methanolic extract 98.1% 97.6% 97.1%* 97.0%* 96.1%*
Control group 98.4% 97.4% 95.9% 93.2% 92.5%
* represent that there is significant difference compared with control group.
Table 3
Sample Bifidobacterium Bacillus acidi lactici Enterobacteriaceae Enterococcus
Water extract 7.04±0.08 5.08±0.10 7.00±0.05* 6.58±0.16*
Ethanol extract 7.30±0.22 5.49±0.17 7.12±0.14* 6.97±0.08*
Methanolic extract 7.08±0.05 5.14±0.14 7.60±0.03 7.06±0.18*
Control group 7.24±0.15 5.68±0.12 8.02±0.09 8.26±0.20
Note:Data are " logarithm value [log (CFU/g colonic contentses)] of every gram of intestinal contents bacteria colony count " in table To represent.* represent that there is significant difference compared with control group.
It can be seen that outer (green grass or young crops) pericarp extract of walnut participates in preparing with antibacterial, the compound of antiviral activity as component Feed addictive.Outer (green grass or young crops) pericarp extract of walnut is preparing the feeding with antibacterial, antiviral activity as sole active agent Application in feed additives.The antibiotic in feed and antiviral drugs can be substituted or reduce its dosage, play and suppress domestic animals And birds common pathogen and virus breeding, regulation gut flora ratio, prophylaxis against infection diseases, the purpose for increasing survival rate. Application in this feed addictive includes poultry fodder and livestock feed.
The present invention provides a kind of green peel of walnut feed addictive, antibiotic and antiviral drugs in alternative feed or Its dosage is reduced, plays and suppresses domestic animals and birds common pathogen and virus breeding, regulation gut flora ratio, prevention infection Property disease, increase survival rate purpose.Application in this feed addictive includes poultry fodder and livestock feed.
The embodiment of the present invention also provides a kind of preparation method of green peel of walnut feed addictive, including following three kinds of methods:
Water extraction method:50g drying green peel of walnut is beaten into powder, 1000ml distilled water refluxing extraction 30min, in triplicate, mistake Filter, merging filtrate is concentrated under reduced pressure, to obtain water extract;
Ethanol extraction method:50g drying green peel of walnut is beaten into powder, 1000ml absolute ethyl alcohol refluxing extraction 30min, repeats three Secondary, filtering, merging filtrate is concentrated under reduced pressure, to obtain ethanol extract;
Methanol extraction:1000ml methanol is oozed into clear extraction 48h, filtering, merging filtrate is concentrated under reduced pressure, to obtain methanol Extract.
Wherein, refluxing extraction can also use one of following solvent:Petroleum ether, ether, dichloromethane, chloroform, second Acetoacetic ester, acetone and other organic solvent.
Preparation method also includes diacolation, backflow, dipping, ultrasound and CO 2 supercritical extraction etc..
Wherein, Extracting temperature is 10-120 DEG C.Condensing mode includes being concentrated under reduced pressure, being freeze-dried.
The present invention is described in detail above, principle and implementation of the specific case used herein to the present invention Mode is set forth, and the explanation of above example is only intended to help the method and its core concept for understanding the present invention;Together When, for those of ordinary skill in the art, according to the thought of the present invention, can in specific embodiments and applications There is change part, in summary, this specification content should not be construed as limiting the invention.

Claims (7)

1. a kind of green peel of walnut feed addictive, it is characterised in that including walnut peel extract.
2. green peel of walnut feed addictive according to claim 1, it is characterised in that also include:It is desmodium 5-10 parts, big Yellow 5-15 parts, radix scutellariae 5-15 parts, golden cypress 10-15 parts, dandelion 5-15 parts, Radix Isatidis 5-15 parts, Rhizoma Sparganii 10-15 parts, curcuma zedoary 5- 15 parts, chrysanthemum indicum 5-15 parts, bighead atractylodes rhizome 5-15 parts, ginseng 10-15 parts.
3. a kind of preparation method of green peel of walnut feed addictive, it is characterised in that including following three kinds of methods:
Water extraction method:50g drying green peel of walnut is beaten into powder, 1000ml distilled water refluxing extraction 30min, in triplicate, filters, closes And filtrate decompression concentrates, to obtain water extract;
Ethanol extraction method:50g drying green peel of walnut is beaten into powder, 1000ml absolute ethyl alcohol refluxing extraction 30min, in triplicate, mistake Filter, merging filtrate is concentrated under reduced pressure, to obtain ethanol extract;
Methanol extraction:1000ml methanol is oozed into clear extraction 48h, filtering, merging filtrate is concentrated under reduced pressure, to obtain methanol extraction Thing.
4. green peel of walnut feed addictive preparation method according to claim 3, it is characterised in that refluxing extraction can be with Using one of following solvent:Petroleum ether, ether, dichloromethane, chloroform, ethyl acetate, acetone and other organic solvent.
5. green peel of walnut feed addictive preparation method according to claim 3, it is characterised in that preparation method also includes Following method:
Diacolation, backflow, dipping, ultrasound and CO 2 supercritical extraction.
6. green peel of walnut feed addictive preparation method according to claim 3, it is characterised in that Extracting temperature 10- 120℃。
7. green peel of walnut feed addictive preparation method according to claim 3, it is characterised in that condensing mode includes subtracting Pressure concentration, freeze-drying.
CN201710611294.5A 2017-07-25 2017-07-25 A kind of green peel of walnut feed addictive and preparation method Pending CN107549465A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110353121A (en) * 2019-07-23 2019-10-22 新疆农业大学 Application and corresponding broiler diet of the plant-derived additive in broiler diet
CN110353120A (en) * 2019-07-23 2019-10-22 新疆农业大学 A kind of plant-derived additive and its application in laying ration
CN110447777A (en) * 2019-07-23 2019-11-15 新疆农业大学 A kind of novel plant lumen fermentation adjusting control agent and the application in sheep daily ration

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766409A (en) * 2012-10-18 2014-05-07 王涵 Walnut green seedcase extract product and bacteriostatic use thereof
US20150297656A1 (en) * 2014-04-16 2015-10-22 Shanghai Zhao Xiang Biological Technology Co., LTD Atractylodes Lancea extract feed additive and preparation method thereof
CN106359878A (en) * 2016-08-29 2017-02-01 黎志春 Preparation method of feed additive

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766409A (en) * 2012-10-18 2014-05-07 王涵 Walnut green seedcase extract product and bacteriostatic use thereof
US20150297656A1 (en) * 2014-04-16 2015-10-22 Shanghai Zhao Xiang Biological Technology Co., LTD Atractylodes Lancea extract feed additive and preparation method thereof
CN106359878A (en) * 2016-08-29 2017-02-01 黎志春 Preparation method of feed additive

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
吴莹等: "核桃 (JuglansregiaL.)青皮提取物抗氧化及抑菌活性的研究", 《畜牧兽医学报》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110353121A (en) * 2019-07-23 2019-10-22 新疆农业大学 Application and corresponding broiler diet of the plant-derived additive in broiler diet
CN110353120A (en) * 2019-07-23 2019-10-22 新疆农业大学 A kind of plant-derived additive and its application in laying ration
CN110447777A (en) * 2019-07-23 2019-11-15 新疆农业大学 A kind of novel plant lumen fermentation adjusting control agent and the application in sheep daily ration

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