CN107519402B - A kind of new process Chinese medicine composition for treating osteopathy - Google Patents
A kind of new process Chinese medicine composition for treating osteopathy Download PDFInfo
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Abstract
The present invention provides a kind of Chinese medicine composition for treating osteopathy, including the component such as rhizoma Gastrodiae, windproof, notopterygium root, reticulate millettia, pueraria lobata, the rhizome of davallia, notopterygium root therein, the rhizome of davallia alcohol reflux after bromelain and cellulase processing extract.
Description
One technical field
The present invention relates to the field of Chinese medicines, specifically, the present invention relates to one kind to treat collar disk and protrusion of lumber intervertebral disc, outer
The Chinese medicine composition of bone injury, rheumatic arthritis caused by wound, which uses enzymatic treatment auxiliary notopterygium root and the rhizome of davallia carries
Take process.
Two background technologies
Fracture belongs to common wound type.At present, doctor trained in Western medicine typically helps the position of bone of patient part when treating fracture
Just, then using medical instrument carried out in it is fixed, or with gypsum, bandage, clamping plate etc. carry out outside fixation, resting slowly,
It is aided with medicine of replenishing the calcium if necessary, does not specifically facilitate the medicine that fracture is recovered.It is many to treat the external application Chinese medicine of fracture, it is generally basede on
Mechanism promoting blood circulation and removing blood stasis, but it is most cut there is uncertain therapeutic efficacy, the shortcomings that effect is not ideal enough.
Disc herniation/bulging is one of refractory chronic disease on Orthopedic Clinical.At present, the treatment of doctor trained in Western medicine to draw,
Based on the physical means such as operation, it is aided with anodyne if necessary, does not specifically facilitate the medicine of interverbebral disc recovery.The traditional Chinese medical science except massage,
Outside the means such as acupuncture, activating blood and removing stasis drug auxiliary treatment generally also can be used, clinically curative effect is better than doctor trained in Western medicine, but still suffers from curative effect
It is imprecise, the shortcomings that effect is poor.
The rhizoma Gastrodiae for being traditionally usually used in such disease, windproof, notopterygium root, chicken blood is commonly used in putting into practice for many years in this hospital
Rattan, pueraria lobata, sand Rhizoma drynariae preparata, radix astragali, Cortex Phellodendri, pseudo-ginseng, berba aristolochiae mollissimae, cassia twig, Shrubalthea Bark, rheum officinale, frutus cnidii, chaulmoogra, Niu Xi,
Ligusticum wallichii configures external Chinese medicament to treat the diseases such as bone injury, disc herniation, synovitis, gout, achieves numerous patients'
Favorable comment.But significant difference is had no with other Chinese patent drugs and prescription according to the medicine drug effect is counted, with traditional compatibility, adjustment dosage, letter
Single means curative effects such as extracting method that change are difficult to further improve, and such self-control medicine component is indefinite, is not suitable for mark
Standardization requirement, it is difficult to be widely applied.
Three content of the invention
According to the demand for clinically strengthening drug effect, applicants studied active ingredient, extracting method in several main components
And the constituent of plant medicinal part, find to destroy cell with bromelain and cellulase processing notopterygium root and the rhizome of davallia
Wall and some intercellular structurals(Speculate, specific mechanism is to be verified)Extraction can unexpectedly strengthen active material extraction effect afterwards
Fruit, and then effectively strengthen drug effect, while unobvious increase prepares complexity and cost.
On the one hand, the present invention provides a kind of Chinese medicine composition for treating osteopathy, including following component:Rhizoma Gastrodiae, windproof, Qiang
Work, reticulate millettia, pueraria lobata, the rhizome of davallia.
Further, wherein the parts by weight of each component are:Rhizoma Gastrodiae 50, windproof 100, notopterygium root 150, reticulate millettia 200, pueraria lobata
200th, the rhizome of davallia 200.
Further, Chinese medicine composition further includes following component:Radix astragali, Cortex Phellodendri, pseudo-ginseng, berba aristolochiae mollissimae, cassia twig, Shrubalthea Bark,
Rheum officinale, frutus cnidii, chaulmoogra, Niu Xi, Ligusticum wallichii.
Further, wherein the parts by weight of each component are:Rhizoma Gastrodiae 50, windproof 100, notopterygium root 150, reticulate millettia 200, pueraria lobata
200th, the rhizome of davallia 200, radix astragali 150, Cortex Phellodendri 300, pseudo-ginseng 150, berba aristolochiae mollissimae 300, cassia twig 50, Shrubalthea Bark 150, rheum officinale 200, cnidium monnieri
Son 150, chaulmoogra 50, ox sunset 200, Ligusticum wallichii 300.
Further, Chinese medicine composition is liquid preparation.
Further, Chinese medicine composition, which is prepared by the following method, forms:
(1)Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight, 200U/g medicinal powder
The cellulase of bromelain and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivators
5min, adds 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h;
(2)By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6
Month, filter to take filtrate;
(3)By step(1)And step(2)The liquid of gained merges, and obtains pharmaceutical composition of the invention.
The Chinese medicine composition of the present invention can be used for bone injury, wind caused by treatment collar disk and protrusion of lumber intervertebral disc, wound
Wet arthritis.
On the other hand, the present invention provides the preparation method of the Chinese medicine composition, including step:
(1)Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water and 200U/g medicinal powder of 5 times of medicinal powder gross weight
Bromelain and 50U/g medicinal powder cellulase, pH is adjusted to react 60min at 6.0,50 DEG C, is heated to 100 DEG C of inactivators
5min, adds 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h;
(2)By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6
Month, filter to take filtrate;
(3)By step(1)And step(2)The liquid of gained merges, and obtains pharmaceutical composition of the invention.
Four brief description of the drawings
Fig. 1 is the representative interverbebral disc state diagram of embodiment 3(It is left:Group 2-4 representativeness states, it is right:Group 1 represents character
State).
Five embodiments
The preparation of 1 Chinese medicine composition of the present invention of embodiment
Weigh rhizoma Gastrodiae 50g, windproof 100g, notopterygium root 150g, reticulate millettia 200g, pueraria lobata 200g, sand Rhizoma drynariae preparata 200g, radix astragali
150g, Cortex Phellodendri 300g, pseudo-ginseng 150g, berba aristolochiae mollissimae 300g, cassia twig 50g, Shrubalthea Bark 150g, rheum officinale 200g, frutus cnidii 150g, strong wind
Sub- 50g, ox sunset 200g, Ligusticum wallichii 300g.
By notopterygium root, sand Rhizoma drynariae preparata, 120 mesh sieves are crossed, add 1700ml water, food-grade bromelain 1.5g(Hunan profit
You are health biology Co., Ltd 50000U/g)With food-grade cellulose element enzyme 1.7g(Hunan Lerkam Co., Ltd., 10000U/
g), pH is adjusted to react 60min at 6.0,50 DEG C, is heated to 100 DEG C of inactivator 5min, adds 80% edible ethanol of 1700ml
Solution, refluxing extraction 1h.
By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 12L height(60 degree)In pure grain wine, seal 3 months, filter to take
Filtrate.
The extracting solution of preceding step and filtrate are merged, obtain Chinese medicine composition of the invention.
Effect of 2 Chinese medicine composition of the present invention of embodiment to rabbit fracture model
The healthy male rabbit 40 of weight 2kg or so is chosen, 4 groups are equally divided into, after Intravenous Anesthesia, in aseptic condition
Lower with medical operation dental burr, horizontal be drilled to wide is about 4mm at epimere 1/3 in the radius of both sides, and the cavity for going deep into marrow forms bone damage
Wound, skin suture.Model is made.
Start medication within postoperative 7th day:
Group 1:Pharmaceutical composition prepared by embodiment 1,3 times a day, about 2ml, is wiped in injury region every time;
Group 2:YUNNAN BAIYAO DING(Yunnan Baiyao group), 3 times a day, about 2ml, is wiped in injury region every time;
Group 3:Hongyao aerosol(Shenyang Hongyao Jingcheng Pharmaceutical Co., Ltd.), 3 times a day, it is sprayed on injury region;
Group 4:Blank control, not medication.
Successive administration 6 weeks, injury region is taken in the 3rd and 6 week every group of weekend execution 6 animal(Containing upper-lower section)Radius with
LUNAR borne densitometers measure bone density.
Experimental result:
The result shows that the plurality of Chinese composition of group 1 to group 3 has certain effect that is promoting blood circulation and removing blood stasis, promoting knitting,
1 bone density value added of group is most obvious, significantly beyond group 2 and group 3, illustrates that the Chinese medicine composition of the present invention can effectively facilitate bone and be cured
Close, effect is better than existing common Exterior-applied Chinese patent medicine.
Effect of 3 Chinese medicine composition of the present invention of embodiment to rabbit protrusion of lumber intervertebral disc model.
The healthy male rabbit 20 of weight 2kg or so is chosen, 4 groups is equally divided into, is fixed on rabbit with special cloth set
60 ° of cervical vertebra flexing, fixes 4h, is freely raised in cage after loosening, continue model to be made in 1 month daily.Start to use from 2nd month
Medicine, continues 2 months, the observation of c spine ap & lat X-ray, primary part observation C4/5, C5/6, C6/7 disc tissue.
Group 1:Pharmaceutical composition prepared by embodiment 1,3 times a day, about 2ml, rubs in affected part every time;
Group 2:The positive pain tincture of toad horse(Shaanxi Ou Ke pharmaceutcal corporation, Ltds), twice daily, about 2ml is rubbed in affected part every time;
Group 3:Return first chinkuning Wet-dressing agent(Tongda Pharmaceutical Co., Ltd., Hangzhou), it is affixed on affected part, each 12h every 1 time on the two.
Group 4:Blank control, not medication.
Iconography shows that group 2-4 interverbebral disc states do not have significant difference, and the interverbebral disc state for organizing 1 is integrally better than a group 2-4,
Fig. 1 is shown in representative interverbebral disc state diagram(It is left:Group 2-4 representativeness states, it is right:The representative state of group 1).
Effect of 4 Chinese medicine composition of the present invention of embodiment to rat arthritis model
The healthy Wistar rats of 30 150g or so are chosen, are equally divided into 3 groups, rat double knee joint chamber injection 4%
Papain solution 0.2ml, was injected 1 time every 3 days, continuous to inject 3 times.Model is made.
Model starts medication on the 2nd after being made
Group 1:Pharmaceutical composition prepared by embodiment 1,3 times a day, each 1ml, rubs in affected part;
Group 2:Double auspicious English 0.009g/kg of vinegar are daily, single administration;
Group 3:Blank control, not medication.
After 4 weeks, abdominal aorta blood sampling, ELISA(Shanghai connection section IL-1 β ELISA kits, reading at microplate reader 450nm)
Detect IL-1 β and TGF-β 1.
Although can not be compared with the Western medicine of definite effect, compared with object, the obvious energy of Chinese medicine composition of the application
It is horizontal (outstanding feature thing in arthritis) to reduce IL-1 β.
The actual clinical effect of 5 Chinese medicine composition of the present invention of embodiment
For privacy confidentiality reasons, case concrete condition and source hospital do not do disclosure.
Case selection:
The similar male's upper limb fracture case 30 of selection situation, age 20-40 Sui, is equally divided into 2 groups.
The similar slight male's lumbar intervertebral disc protrusion example 20 of selection situation, age 40-50 Sui, is equally divided into 2 groups.
The similar slight rheumatoid arthritis disease example 30 of selection situation, age 40-50 Sui, is equally divided into 2 groups.
Therapeutic scheme:
All fracture and protrusion of lumber intervertebral disc patient receive fixation and the traction therapeutic of standard, fracture and protrusion of lumber intervertebral disc
Respectively there is one group of pharmaceutical composition prepared using embodiment 1,3 times a day, about 10ml, is wiped in injury region every time(Fracture patient is consolidated
Begin to use within 3 weeks after fixed processing, protrusion of lumber intervertebral disc patient uses daily from beginning to end).Observation comparison is carried out after 3 months.
Whole rheumatoid arthritis patients use clinical common FENGSHI GUANJIEYAN PIAN(The limited public affairs of Shanxi Hua Kang medicine company shares
Department)Treatment, one of which add the pharmaceutical composition prepared with embodiment 1, and 3 times a day, about 10ml, is wiped in injury region every time.3
Observation comparison is carried out after month.
Observation of curative effect:
Clinical imageology and bone density evidence show that the medicine of the application has the effect of obvious aids fracture reparation(Wound
Place's mean bone density is higher than non-medication group more than 15%);The iconography and readme symptom of the protrusion of lumber intervertebral disc patient of medication also compared with
Non- medication group is more preferable;Add and be also generally better than list with rheumatoid arthritis patients' readme of Chinese medicine composition of the present invention and clinical score
Solely use the patient of FENGSHI GUANJIEYAN PIAN.
The Chinese medicine composition drug effect of the present invention of embodiment 6 improves mechanism study
In order to prove and further study the extraction effect of enzymatic treatment assisted extraction method proposed by the present invention, carry out following
The more a variety of notopterygium roots of experiment and the extracting method of the rhizome of davallia:
Predominantly detect equipment:
1100 type chromatographs of Aglient, 753 spectrophotometers
Main agents and sample:
Nodakenin, Notopterol, aurantiin standard items are purchased from Aladdin industrial corporation;
Sample 1:The notopterygium root Drynaria Rhizome extracted according to 1 step of embodiment(To avoid more multicomponent influence, not with
With regard to immersion filtrate mixing);
Sample 2:Extracted 3 times with 8 times of 60% alcohol refluxs of weight, the sand Rhizoma drynariae preparata extracting solution of each 1h;
Sample 3:Extracted 3 times with 8 times of 80% alcohol refluxs of weight, the notopterygium root extracting solution of each 1h;
Sample 4:According to 1 step of embodiment, without enzymatic treatment, the notopterygium root Drynaria Rhizome of extraction.
Nodakenin, Notopterol, general flavone, the assay method of aurantiin:
Nodakenin and each 5 mg of Notopterol standard items are weighed, is respectively placed in 5mL volumetric flasks, is matched somebody with somebody with methanol constant volume
Reference substance storing solution processed.Appropriate reference substance storing solution is drawn respectively to mix and use methanol constant volume to be made mixed in 5mL volumetric flasks
Close reference substance storing solution.Accurate mixing reference substance 10 different quality concentration of storing solution of drawing successively carry out HPLC analysis detections peak
Area, using mass concentration as abscissa X, average peak area carries out linear regression for ordinate Y, and regression equation is y=83565x+
28991、y=31934x+46537;
Aurantiin reference substance 5mg is weighed, is placed in 50mL volumetric flasks, prepares reference substance solution with methanol constant volume, then distinguish
0.5,1.0,1.5,2.0,2.5,3.0 ml of reference substance solution is drawn in 10ml volumetric flasks, methanol constant volume, using methanol as blank,
Trap is surveyed at 283 nm, using concentration as abscissa X, trap is ordinate Y, and regression equation is: y=0.025x+
0.013;
Aurantiin standard items 5mg is weighed, is placed in 5mL volumetric flasks, reference substance storing solution is prepared with methanol constant volume.It is accurate according to
Secondary mixing reference substance 10 different quality concentration of storing solution of drawing carry out HPLC analysis detection peak areas, using mass concentration as horizontal seat
X is marked, average peak area carries out linear regression for ordinate Y, and regression equation is:y=28631x+62227.
With the nodakenin in above-mentioned standard curve and regression equation calculation each sample, Notopterol, Notopterol, aurantiin
(Sample 2 only measures general flavone, aurantiin, and sample 3 only measures nodakenin, Notopterol)And calculate and be reduced to Unit Weight Qiang
Active ingredient weight obtained by the living and rhizome of davallia is as a result as follows:
As it can be seen that the processing that the application adds bromelain and cellulase greatly improves notopterygium root in from the above
With the extraction efficiency of nodakenin, Notopterol, Notopterol, aurantiin in the rhizome of davallia(Each component only 1h refluxing extractions are with regard to that can reach
To 1.5-1.8 times of simple 3 1h effects of refluxing extraction, from the point of view of active ingredient weight obtained by Unit Weight notopterygium root and the rhizome of davallia
Also apparently higher than numerical value disclosed in existing literature), while ensure that extraction cost hardly raises(Food-grade bromelain and
Cellulase is cheap, and dosage is also very low;3h of total processing time 2h also below traditional 3 refluxing extractions).For carrying for drug effect
Height provides strong guarantee.
Claims (5)
1. a kind of Chinese medicine composition for treating osteopathy, is made of the component of following parts by weight:Rhizoma Gastrodiae 50, windproof 100, notopterygium root
150th, reticulate millettia 200, pueraria lobata 200, the rhizome of davallia 200, radix astragali 150, Cortex Phellodendri 300, pseudo-ginseng 150, berba aristolochiae mollissimae 300, cassia twig 50, the river rose of Sharon
Skin 150, rheum officinale 200, frutus cnidii 150, chaulmoogra 50, the root of bidentate achyranthes 200, Ligusticum wallichii 300;The Chinese medicine composition is prepared by the following method
Form:(1)Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight, the spinach of 200U/g medicinal powder
The cellulase of trailing plants protease and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivators
5min, adds 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h;(2)By remaining pulverizing medicinal materials, 100 are crossed
Mesh sieve, is soaked in 5 times of height pure grain wines of medicinal powder gross weight, and sealing 3-6 months, filters to take filtrate;
(3)By step(1)And step(2)The liquid of gained merges, and obtains the Chinese medicine composition.
2. purposes of the Chinese medicine composition of claim 1 in the medicine for the treatment of collar disk and protrusion of lumber intervertebral disc is prepared.
3. purposes of the Chinese medicine composition of claim 1 in the medicine of bone injury caused by treatment wound is prepared.
4. purposes of the Chinese medicine composition of claim 1 in the medicine for preparing treatment rheumatic arthritis.
5. the preparation method of the Chinese medicine composition of claim 1, including step:
(1)Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight, the pineapple of 200U/g medicinal powder
The cellulase of protease and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivator 5min,
Add 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h;
(2)By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6 months,
Filter to take filtrate;
(3)By step(1)And step(2)The liquid of gained merges, and obtains the Chinese medicine composition.
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