CN107519402A - A kind of new technology Chinese medicine composition for treating osteopathy - Google Patents
A kind of new technology Chinese medicine composition for treating osteopathy Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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Abstract
The invention provides a kind of Chinese medicine composition for treating osteopathy, including the composition such as rhizoma Gastrodiae, windproof, notopterygium root, reticulate millettia, the root of kudzu vine, the rhizome of davallia, notopterygium root therein, the rhizome of davallia alcohol reflux after bromelain and cellulase processing extract.
Description
One technical field
The present invention relates to the field of Chinese medicines, specifically, treats collar disk the present invention relates to one kind and protrusion of lumber intervertebral disc, wound are drawn
Rise bone injury, the Chinese medicine composition of rheumatic arthritis, which use ferment treatment auxiliary notopterygium root and the rhizome of davallia extraction
Journey.
Two background technologies
Fracture belongs to common wound type.At present, doctor trained in Western medicine treat fracture when, typically by the position of bone righting of patient part, so
Afterwards using medicine equipment carried out in it is fixed, or with gypsum, bandage, clamping plate etc. carry out outside fixation, resting slowly, if necessary
It is aided with medicine of replenishing the calcium, does not specifically facilitate the medicine that fracture is recovered.It is many to treat the external application Chinese medicine of fracture, it is generally basede on promoting blood circulationization
The mechanism of the stasis of blood, but it is most cut there is uncertain therapeutic efficacy, the shortcomings that effect is not ideal enough.
Disc herniation/bulging is one of refractory chronic disease on Orthopedic Clinical.At present, the treatment of doctor trained in Western medicine to draw,
Based on the physical means such as operation, it is aided with anodyne if necessary, does not specifically facilitate the medicine of interverbebral disc recovery.The traditional Chinese medical science except massage,
Outside the means such as acupuncture, activating blood and removing stasis drug auxiliary treatment typically also can be used, clinically curative effect is better than doctor trained in Western medicine, but still suffers from curative effect
It is imprecise, the shortcomings that effect is poor.
The rhizoma Gastrodiae for being traditionally usually used in such disease, windproof, notopterygium root, chicken blood is commonly used in putting into practice for many years in this hospital
Rattan, the root of kudzu vine, sand Rhizoma drynariae preparata, the Radix Astragali, golden cypress, pseudo-ginseng, berba aristolochiae mollissimae, cassia twig, Shrubalthea Bark, rheum officinale, frutus cnidii, chaulmoogra, Niu Xi,
Ligusticum wallichii configures external Chinese medicament to treat the diseases such as bone injury, disc herniation, synovitis, gout, achieves numerous patients'
Favorable comment.But significant difference is had no with other Chinese patent drugs and prescription according to the medicine drug effect is counted, with traditional compatibility, adjustment dosage, letter
Single means curative effects such as extracting method that change are difficult to further improve, and such self-control medicine component is indefinite, is not suitable for mark
Standardization requirement, it is difficult to be widely applied.
Three content of the invention
According to the demand for clinically strengthening drug effect, applicants studied active component in several main components, extracting method and
The constituent of plant medicinal part, find with bromelain and cellulase processing notopterygium root and the rhizome of davallia destroy cell membrane and
Some intercellular structurals（Speculate, specific mechanism is to be verified）Extraction can unexpectedly strengthen active material extraction effect afterwards, enter
And effectively strengthen drug effect, while unobvious increase prepares complexity and cost.
On the one hand, the invention provides a kind of Chinese medicine composition for treating osteopathy, including following component：Rhizoma Gastrodiae, windproof, Qiang
Work, reticulate millettia, the root of kudzu vine, the rhizome of davallia.
Further, wherein the parts by weight of each composition are：Rhizoma Gastrodiae 50, windproof 100, notopterygium root 150, reticulate millettia 200, the root of kudzu vine
200th, the rhizome of davallia 200.
Further, Chinese medicine composition also includes following component：The Radix Astragali, golden cypress, pseudo-ginseng, berba aristolochiae mollissimae, cassia twig, Shrubalthea Bark,
Rheum officinale, frutus cnidii, chaulmoogra, Niu Xi, Ligusticum wallichii.
Further, wherein the parts by weight of each composition are：Rhizoma Gastrodiae 50, windproof 100, notopterygium root 150, reticulate millettia 200, the root of kudzu vine
200th, the rhizome of davallia 200, the Radix Astragali 150, golden cypress 300, pseudo-ginseng 150, berba aristolochiae mollissimae 300, cassia twig 50, Shrubalthea Bark 150, rheum officinale 200, cnidium monnieri
Son 150, chaulmoogra 50, ox sunset 200, Ligusticum wallichii 300.
Further, Chinese medicine composition is liquid preparation.
Further, Chinese medicine composition, which is prepared by the following method, forms：
（1）Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight, the pineapple of 200U/g medicinal powder
The cellulase of protease and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivator 5min,
Add 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h；
（2）By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6 months,
Filter to take filtrate；
（3）By step（1）And step（2）The liquid of gained merges, and obtains pharmaceutical composition of the invention.
The Chinese medicine composition of the present invention can be used for bone injury, wind caused by treatment collar disk and protrusion of lumber intervertebral disc, wound
Wet arthritis.
On the other hand, the invention provides the preparation method of the Chinese medicine composition, including step：
（1）Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight and the spinach of 200U/g medicinal powder
The cellulase of trailing plants protease and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivators
5min, add 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h；
（2）By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6 months,
Filter to take filtrate；
（3）By step（1）And step（2）The liquid of gained merges, and obtains pharmaceutical composition of the invention.
Four brief description of the drawings
Fig. 1 is the representative interverbebral disc state diagram of embodiment 3（It is left：Group 2-4 representativeness states, it is right：1 representative state of group）.
Five embodiments
The preparation of 1 Chinese medicine composition of the present invention of embodiment
Weigh rhizoma Gastrodiae 50g, windproof 100g, notopterygium root 150g, reticulate millettia 200g, root of kudzu vine 200g, sand Rhizoma drynariae preparata 200g, the Radix Astragali
150g, golden cypress 300g, pseudo-ginseng 150g, berba aristolochiae mollissimae 300g, cassia twig 50g, Shrubalthea Bark 150g, rheum officinale 200g, frutus cnidii 150g, strong wind
Sub- 50g, ox sunset 200g, Ligusticum wallichii 300g.
By notopterygium root, sand Rhizoma drynariae preparata, 120 mesh sieves are crossed, add 1700ml water, food-grade bromelain 1.5g（Hunan profit
You are health biology Co., Ltd 50000U/g）With food-grade cellulose element enzyme 1.7g（Hunan Lerkam Co., Ltd., 10000U/
g）, pH is adjusted to react 60min at 6.0,50 DEG C, is heated to 100 DEG C of inactivator 5min, adds 1700ml 80% edible ethanol
Solution, refluxing extraction 1h.
By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 12L height（60 degree）In pure grain wine, seal 3 months, filter to take
Filtrate.
The extract solution of preceding step and filtrate are merged, obtain Chinese medicine composition of the invention.
Effect of 2 Chinese medicine composition of the present invention of embodiment to rabbit fracture model
Body weight 2kg or so healthy male rabbit 40 is chosen, 4 groups is equally divided into, after Intravenous Anesthesia, aseptically uses
Horizontal be drilled to wide is about 4mm to medical operation dental burr at epimere 1/3 in the radius of both sides, and the cavity for going deep into marrow forms bone injury,
Skin suture.Model is made.
Start medication within postoperative 7th day：
Group 1：Pharmaceutical composition prepared by embodiment 1,3 times a day, about 2ml, is wiped in injury region every time；
Group 2：YUNNAN BAIYAO DING（Yunnan Baiyao group）, 3 times a day, about 2ml, is wiped in injury region every time；
Group 3：Hongyao aerosol（Shenyang Hongyao Jingcheng Pharmaceutical Co., Ltd.）, 3 times a day, it is sprayed on injury region；
Group 4：Blank control, not medication.
Successive administration 6 weeks, injury region is taken in the 3rd and 6 week every group of weekend execution 6 animal（Containing upper-lower section）Radius with
LUNAR borne densitometers determine bone density.
Experimental result：
As a result showing the plurality of Chinese composition of group 1 to group 3 has certain effect that is promoting blood circulation and removing blood stasis, promoting knitting, organizes 1 bone
Density value added is most obvious, and significantly beyond group 2 and group 3, knitting, effect can be effectively facilitated by illustrating the Chinese medicine composition of the present invention
It is better than existing conventional Exterior-applied Chinese patent medicine.
Effect of 3 Chinese medicine composition of the present invention of embodiment to rabbit protrusion of lumber intervertebral disc model.
Body weight 2kg or so healthy male rabbit 20 is chosen, 4 groups is equally divided into, is fixed on rabbit with special cloth set
60 ° of cervical vertebra flexing, 4h is fixed daily, is freely raised in cage after loosening, continues model to be made in 1 month.Start to use from 2nd month
Medicine, continue 2 months, the observation of c spine ap ＆ lat X ray, primary part observation C4/5, C5/6, C6/7 disc tissue.
Group 1：Pharmaceutical composition prepared by embodiment 1,3 times a day, about 2ml, is rubbed in affected part every time；
Group 2：The positive pain tincture of toad horse（Shaanxi Ou Ke pharmaceutcal corporation, Ltds）, twice daily, about 2ml is rubbed in affected part every time；
Group 3：Return first chinkuning Wet-dressing agent（Tongda Pharmaceutical Co., Ltd., Hangzhou）, it is affixed on affected part, each 12h every 1 time on the two.
Group 4：Blank control, not medication.
Iconography shows that group 2-4 interverbebral disc states do not have significant difference, and the interverbebral disc state for organizing 1 is integrally better than a group 2-4,
Fig. 1 is shown in representative interverbebral disc state diagram（It is left：Group 2-4 representativeness states, it is right：1 representative state of group）.
Effect of 4 Chinese medicine composition of the present invention of embodiment to rat arthritis model
30 150g or so healthy Wistar rats are chosen, are equally divided into 3 groups, the pawpaw of rat double knee joint chamber injection 4%
Protein enzyme solution 0.2ml, injected 1 time every 3 days, it is continuous to inject 3 times.Model is made.
Model starts medication on the 2nd after being made
Group 1：Pharmaceutical composition prepared by embodiment 1,3 times a day, each 1ml, is rubbed in affected part；
Group 2：Double auspicious English 0.009g/kg of vinegar are daily, single administration；
Group 3：Blank control, not medication.
After 4 weeks, abdominal aorta blood sampling, ELISA（Shanghai connection section IL-1 β ELISA kits, reading at ELIASA 450nm）
Detect IL-1 β and TGF-β 1.
Although can not be compared with the Western medicine of definite effect, compared with object, the obvious energy of Chinese medicine composition of the application
It is horizontal (outstanding feature thing in arthritis) to reduce IL-1 β.
The actual clinical effect of 5 Chinese medicine composition of the present invention of embodiment
For privacy confidentiality reasons, case concrete condition and source hospital do not do disclosure.
Case selection：
The similar male's upper limb fracture case 30 of selection situation, age 20-40 year, is equally divided into 2 groups.
The similar slight male's lumbar intervertebral disc protrusion example 20 of selection situation, age 40-50 year, is equally divided into 2 groups.
The similar slight rheumatoid arthritis disease example 30 of selection situation, age 40-50 year, is equally divided into 2 groups.
Therapeutic scheme：
All fracture and protrusion of lumber intervertebral disc patient receive fixation and the traction therapeutic of standard, and fracture and protrusion of lumber intervertebral disc respectively have
One group of pharmaceutical composition prepared using embodiment 1,3 times a day, about 10ml, is wiped in injury region every time（Fracture patient bearing
Begin to use within 3 weeks after reason, protrusion of lumber intervertebral disc patient uses daily from beginning to end）.Observation comparison is carried out after 3 months.
Whole rheumatoid arthritis patients use clinical conventional FENGSHI GUANJIEYAN PIAN（The limited public affairs of Shanxi Hua Kang medicine company shares
Department）Treatment, one of which add the pharmaceutical composition prepared with embodiment 1, and 3 times a day, about 10ml, is wiped in injury region every time.3
Observation comparison is carried out after month.
Observation of curative effect：
Clinical imageology and bone density evidence show that the medicine of the application has the effect of obvious aids fracture reparation（Put down injury
Equal bone density is higher than non-medication group more than 15%）；The iconography and readme symptom of the protrusion of lumber intervertebral disc patient of medication is also more unused
Medicine group is more preferable；Adding also generally to be better than with rheumatoid arthritis patients' readme of Chinese medicine composition of the present invention and clinical score individually makes
With the patient of FENGSHI GUANJIEYAN PIAN.
The Chinese medicine composition drug effect of the present invention of embodiment 6 improves study mechanism
In order to prove and further study the extraction effect of ferment treatment assisted extraction method proposed by the present invention, following test is carried out
More a variety of notopterygium roots and the extracting method of the rhizome of davallia：
Predominantly detect equipment：
The type chromatographs of Aglient 1100,753 spectrophotometers
Main agents and sample：
Nodakenin, Notopterol, aurantiin standard items are purchased from Aladdin industrial corporation；
Sample 1：The notopterygium root Drynaria Rhizome extracted according to the step of embodiment 1（To avoid more multicomponent influence, not with just soaking
Steep filtrate mixing）；
Sample 2：Extracted 3 times with 8 times of alcohol refluxs of weight 60%, each 1h sand Rhizoma drynariae preparata extract solution；
Sample 3：Extracted 3 times with 8 times of alcohol refluxs of weight 80%, each 1h notopterygium root extract solution；
Sample 4：According to the step of embodiment 1, without ferment treatment, the notopterygium root Drynaria Rhizome of extraction.
Nodakenin, Notopterol, general flavone, the assay method of aurantiin：
Nodakenin and each 5 mg of Notopterol standard items are weighed, is respectively placed in 5mL volumetric flasks, with methanol constant volume preparation pair
According to product storing solution.Appropriate reference substance storing solution is drawn respectively to mix and use methanol constant volume in 5mL volumetric flasks, and mixing pair is made
According to product storing solution.Accurate mixing reference substance 10 different quality concentration of storing solution of drawing successively carry out HPLC analysis detections peak face
Product, using mass concentration as abscissa X, average peak area is that ordinate Y carries out linear regression, and regression equation is y=83565x+
28991、y=31934x+46537；
Aurantiin reference substance 5mg is weighed, is placed in 50mL volumetric flasks, prepares reference substance solution with methanol constant volume, then draw respectively
Ml is in 10ml volumetric flasks for reference substance solution 0.5,1.0,1.5,2.0,2.5,3.0, methanol constant volume, using methanol as blank,
Trap is surveyed at 283 nm, using concentration as abscissa X, trap is ordinate Y, and regression equation is: y=0.025x+0.013；
Aurantiin standard items 5mg is weighed, is placed in 5mL volumetric flasks, reference substance storing solution is prepared with methanol constant volume.Precision is inhaled successively
Mixing reference substance 10 different quality concentration of storing solution are taken to carry out HPLC analysis detection peak areas, using mass concentration as abscissa X
, average peak area is that ordinate Y carries out linear regression, and regression equation is：y=28631x+62227.
With the nodakenin in above-mentioned standard curve and regression equation calculation each sample, Notopterol, Notopterol, aurantiin
（Sample 2 only determines general flavone, aurantiin, and sample 3 only determines nodakenin, Notopterol）And calculate and be reduced to Unit Weight Qiang
Active component weight obtained by the living and rhizome of davallia is as a result as follows：
Visible in from the above, the processing that the application adds bromelain and cellulase greatly improves notopterygium root and bone
Broken bowl spares nodakenin, Notopterol, Notopterol, the extraction efficiency of aurantiin（Each composition only 1h refluxing extractions are with regard to that can reach single
1.5-1.8 times of pure 3 1h effects of refluxing extraction, it is also bright from the point of view of active component weight obtained by Unit Weight notopterygium root and the rhizome of davallia
It is aobvious to be higher than numerical value disclosed in existing literature）, while ensure that extraction cost hardly raises（Food-grade bromelain and fiber
Plain enzyme is cheap, and dosage is also very low；3h of total processing time 2h also below traditional 3 refluxing extractions）.Carried for the raising of drug effect
Strong guarantee is supplied.
Claims (10)
1. a kind of Chinese medicine composition for treating osteopathy, including following component：Rhizoma Gastrodiae, windproof, notopterygium root, reticulate millettia, the root of kudzu vine, bone are broken
Mend.
2. Chinese medicine composition according to claim 1, wherein the parts by weight of each composition are：Rhizoma Gastrodiae 50, windproof 100, notopterygium root
150th, reticulate millettia 200, the root of kudzu vine 200, the rhizome of davallia 200.
3. Chinese medicine composition according to claim 1, in addition to following component：The Radix Astragali, golden cypress, pseudo-ginseng, berba aristolochiae mollissimae, cassia twig, river
Rose of Sharon skin, rheum officinale, frutus cnidii, chaulmoogra, Niu Xi, Ligusticum wallichii.
4. Chinese medicine composition according to claim 3, wherein the parts by weight of each composition are：Rhizoma Gastrodiae 50, windproof 100, notopterygium root
150th, reticulate millettia 200, the root of kudzu vine 200, the rhizome of davallia 200, the Radix Astragali 150, golden cypress 300, pseudo-ginseng 150, berba aristolochiae mollissimae 300, cassia twig 50, the river rose of Sharon
Skin 150, rheum officinale 200, frutus cnidii 150, chaulmoogra 50, ox sunset 200, Ligusticum wallichii 300.
5. any one of claim 1-4 Chinese medicine composition, it is liquid preparation for external application.
6. any one of claim 1-5 Chinese medicine composition, it, which is prepared by the following method, forms：
（1）Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight, the pineapple of 200U/g medicinal powder
The cellulase of protease and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivator 5min,
Add 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h；
（2）By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6 months,
Filter to take filtrate；
（3）By step（1）And step（2）The liquid of gained merges, and obtains pharmaceutical composition of the invention.
7. any one of claim 1-6 Chinese medicine composition, it is used to treat collar disk and protrusion of lumber intervertebral disc.
8. any one of claim 1-6 Chinese medicine composition, it is used to treat bone injury caused by wound.
9. any one of claim 1-6 Chinese medicine composition, it is used to treat rheumatic arthritis.
10. the preparation method of any one of claim 1-6 Chinese medicine composition, including step：
（1）Notopterygium root, sand Rhizoma drynariae preparata are crushed, cross 120 mesh sieves, adds the water of 5 times of medicinal powder gross weight, the pineapple of 200U/g medicinal powder
The cellulase of protease and 50U/g medicinal powder, pH are adjusted to react 60min at 6.0,50 DEG C, are heated to 100 DEG C of inactivator 5min,
Add 80% edible ethanol solution of 5 times of medicinal powder gross weight, refluxing extraction 1h；
（2）By remaining pulverizing medicinal materials, 100 mesh sieves are crossed, are soaked in 5 times of height pure grain wines of medicinal powder gross weight, sealing 3-6 months,
Filter to take filtrate；
（3）By step（1）And step（2）The liquid of gained merges, and obtains pharmaceutical composition of the invention.
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| CN108498593A (en) * | 2018-06-25 | 2018-09-07 | 高金儿 | A kind of Chinese medicine composition for treating neck-shoulder pain, lumbago and leg pain |
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