CN107495079A - A kind of method for preparing selenium-rich natto - Google Patents

A kind of method for preparing selenium-rich natto Download PDF

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Publication number
CN107495079A
CN107495079A CN201710810024.7A CN201710810024A CN107495079A CN 107495079 A CN107495079 A CN 107495079A CN 201710810024 A CN201710810024 A CN 201710810024A CN 107495079 A CN107495079 A CN 107495079A
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CN
China
Prior art keywords
natto
selenium
rich
semi
fermentation tank
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Pending
Application number
CN201710810024.7A
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Chinese (zh)
Inventor
孔维海
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Nanjing Royal Plaque Biotechnology Co Ltd
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Nanjing Royal Plaque Biotechnology Co Ltd
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Priority to CN201710810024.7A priority Critical patent/CN107495079A/en
Publication of CN107495079A publication Critical patent/CN107495079A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10General methods of cooking foods, e.g. by roasting or frying
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a kind of method for preparing selenium-rich natto.This method comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried standby;Step 2, the soya bean after cleaning is put into fermentation tank, access Bacillus subtilis natto from Traditional Japanese Food ferments after 1 4h, takes out the natto semi-finished product with white silk;Step 3, natto semi-finished product are entered in catalytic mixing liquid after the 40h of deepfreeze 30, it is standby after the 3min of plasma treatment 1;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues 1 1.5h that ferments, produce selenium-rich natto.Compared with prior art, preparation method of the present invention is simple, using multistage fermentation and plasma treatment, improves the fermentation yield of natto, and the quality of natto.

Description

A kind of method for preparing selenium-rich natto
Technical field
The present invention relates to natto preparation field, and in particular to a kind of method for preparing selenium-rich natto.
Background technology
Natto be by fermenting bacillus natto soybean and made of functional food.Numerous studies show, are removed in natto and are rich in ammonia Base acid, organic acid, outside oligosaccharides, in addition to several physiological active substances, as Nattokinase can dissolve thrombus, mainly effectively dissolving It is insoluble.But Nattokinase is active relatively low in traditional natto product, and the duration is short, and thrombolytic effect is effective.
Application number 201611095448.1, a kind of entitled preparation method of natto grain, this method comprise the following steps: (1)Selected chick-pea is crushed, crosses 60 mesh sieves, then plus water cooks;(2)Treat that boiling is cooled to 37 DEG C, be inoculated with withered grass gemma Bacillus carries out heat-preservation fermentation;(3)After fermentation, by fermented liquid spray drying, 60 mesh sieves are then crossed, obtain natto powder;(4)It will receive After bean powder mixes with auxiliary material, granulation, then it is dried, obtains dry particle;(5)Dried particle is subjected to tabletting, Obtain natto grain;Step(4)The weight ratio of middle natto powder and auxiliary material is 50-55:45-50, the auxiliary material is by microcrystalline cellulose, hydroxyl Third methylcellulose and ethanol composition.But the yield of the natto has much room for improvement.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of method for preparing selenium-rich natto, this method Simple and easy, Nattokinase content is high in natto.
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 1-4h, taken out with white silk Natto semi-finished product;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 30-40h, plasma treatment 1- It is standby after 3min;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues the 1-1.5h that ferments, produce selenium-rich Natto.
It is that temperature is dried in step 1 for 30-45 DEG C as improved.
It is that the mixing speed of fermentation tank is 100-120rpm in step 2 as improved, oxygen ventilation amount is 0.03- 0.08L/(min·L), fermentation temperature is 40-60 DEG C.
It is that the inoculum concentration of Bacillus subtilis natto from Traditional Japanese Food is 1 in step 2 as improved:500-700.
It is as improved, the mixing that catalytic mixing liquid described in step 3 is made up of Organic Selenium, citric acid and deionized water The weight ratio of thing, wherein Organic Selenium, citric acid and deionized water is 1:2-3:1-4.
It is that the frequency of plasma treatment is 1200-1500HZ in step 3, and the temperature of deepfreeze is 10- as improved 15℃。
As it is improved when, oxygen content maintains 80-85% in fermentation tank in step 4.
Compared with prior art, preparation method of the present invention is simple, using multistage fermentation and plasma treatment, improves natto Fermentation yield, and the content of Nattokinase is increased in the quality of natto, especially natto.
Embodiment
The present invention is further described in detail below by specific embodiment.
Embodiment 1
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried at 30 DEG C standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 1h, takes out receiving with white silk Beans semi-finished product;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 30h, plasma treatment 1min standby With;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues the 1h that ferments, produce selenium-rich natto.
Wherein, the mixing speed of fermentation tank is 100rpm in step 2, and oxygen ventilation amount is 0.03L/(min·L), fermentation Temperature is 40 DEG C.
The inoculum concentration of Bacillus subtilis natto from Traditional Japanese Food is 1 in step 2:500.
The mixture that catalytic mixing liquid described in step 3 is made up of Organic Selenium, citric acid and deionized water, wherein organic The weight ratio of selenium, citric acid and deionized water is 1:2:1.
The frequency of plasma treatment is 1200HZ in step 3, and the temperature of deepfreeze is 10 DEG C.
Oxygen content maintains 80% in fermentation tank in step 4.
Embodiment 2
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried at 40 DEG C standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 2h, takes out receiving with white silk Beans semi-finished product;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 35h, plasma treatment 2min standby With;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues the 1.2h that ferments, produce selenium-rich natto.
Wherein, the mixing speed of fermentation tank is 110rpm in step 2, and oxygen ventilation amount is 0.05L/(min·L), fermentation Temperature is 42 DEG C.
The inoculum concentration of Bacillus subtilis natto from Traditional Japanese Food is 1 in step 2:600.
The mixture that catalytic mixing liquid described in step 3 is made up of Organic Selenium, citric acid and deionized water, wherein organic The weight ratio of selenium, citric acid and deionized water is 1:2.4:3.
The frequency of plasma treatment is 1300HZ in step 3, and the temperature of deepfreeze is 12 DEG C.
Oxygen content maintains 82% in fermentation tank in step 4.
Embodiment 3
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried at 45 DEG C standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 4h, takes out receiving with white silk Beans semi-finished product;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 40h, plasma treatment 3min standby With;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues the 1.5h that ferments, produce selenium-rich natto.
Wherein, the mixing speed of fermentation tank is 120rpm in step 2, and oxygen ventilation amount is 0.08L/(min·L), fermentation Temperature is 60 DEG C.
The inoculum concentration of Bacillus subtilis natto from Traditional Japanese Food is 1 in step 2: 700.
The mixture that catalytic mixing liquid described in step 3 is made up of Organic Selenium, citric acid and deionized water, wherein organic The weight ratio of selenium, citric acid and deionized water is 1:3: 4.
The frequency of plasma treatment is 1200-1500HZ in step 3, and deepfreeze temperature is 15 DEG C.
Oxygen content maintains 85% in fermentation tank in step 4.
Embodiment 4
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried at 30 DEG C standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 1h, takes out receiving with white silk Beans semi-finished product;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 30h, plasma treatment 1min standby With;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues the 1h that ferments, produce selenium-rich natto.
Wherein, the mixing speed of fermentation tank is 120rpm in step 2, and oxygen ventilation amount is 0.08L/(min·L), fermentation Temperature is 60 DEG C.
The inoculum concentration of Bacillus subtilis natto from Traditional Japanese Food is 1 in step 2: 700.
The mixture that catalytic mixing liquid described in step 3 is made up of Organic Selenium, citric acid and deionized water, wherein organic The weight ratio of selenium, citric acid and deionized water is 1:3: 4.
The frequency of plasma treatment is 1200-1500HZ in step 3, and deepfreeze temperature is 15 DEG C.
Oxygen content maintains 85% in fermentation tank in step 4.
Embodiment 5
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried at 45 DEG C standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 4h, takes out receiving with white silk Beans semi-finished product;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 40h, plasma treatment 3min standby With;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continues the 1.5h that ferments, produce selenium-rich natto.
Wherein, the mixing speed of fermentation tank is 100rpm in step 2, and oxygen ventilation amount is 0.03L/(min·L), fermentation Temperature is 40 DEG C.
The inoculum concentration of Bacillus subtilis natto from Traditional Japanese Food is 1 in step 2:500.
The mixture that catalytic mixing liquid described in step 3 is made up of Organic Selenium, citric acid and deionized water, wherein organic The weight ratio of selenium, citric acid and deionized water is 1:2:1.
The frequency of plasma treatment is 1200HZ in step 3, and the temperature of deepfreeze is 10 DEG C.
Oxygen content maintains 80% in fermentation tank in step 4.
Comparative example 1
A kind of method for preparing selenium-rich natto, comprises the following steps:Step 1, the soya bean being of moderate size is selected, is dried at 40 DEG C standby With;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 2h, takes out receiving with white silk Beans.
Remaining is the same as embodiment 2.
The natto quality of embodiment 1-5 and comparative example 1 is detected, the data obtained is as shown in the table.
It is seen from the above data that the natto quality of the present invention is good, and fermentation operation is convenient.
Typical case:
100 nattos that embodiment 2 and comparative example 1 are taken with thrombosis patients of selection, adhere to after taking 30 days by daily two, Taking in the natto of embodiment 2 50 has 48 patient symptoms to mitigate, and 38 patients' recoveries from illness, takes 50 in the natto of comparative example 1 There are 28 patient symptoms to mitigate in name, 15 patient's recoveries from illness.Therefore, selenium-rich Nattokinase is substantially increased and received in natto of the present invention Beans cure ability.
In addition, the invention is not restricted to above-mentioned embodiment, as long as in without departing from the scope of the present invention, can take various Mode implements the present invention.

Claims (7)

  1. A kind of 1. method for preparing selenium-rich natto, it is characterised in that comprise the following steps:Step 1, the Huang being of moderate size is selected Beans, dry standby;Step 2, the soya bean after cleaning is put into fermentation tank, after access Bacillus subtilis natto from Traditional Japanese Food fermentation 1-4h, taken out Natto semi-finished product with white silk;Step 3, natto semi-finished product are entered in catalytic mixing liquid after deepfreeze 30-40h, wait from It is standby after subprocessing 1-3min;Step 4, the natto semi-finished product of step 3 are put into new fermentation tank, continue the 1-1.5h that ferments, Produce selenium-rich natto.
  2. 2. a kind of method for preparing selenium-rich natto according to claim 1, it is characterised in that temperature is dried in step 1 is 30-45℃。
  3. 3. a kind of method for preparing selenium-rich natto according to claim 1, it is characterised in that fermentation tank stirs in step 2 It is 100-120rpm to mix speed, and oxygen ventilation amount is 0.03-0.08L/(min·L), fermentation temperature is 40-60 DEG C.
  4. A kind of 4. method for preparing selenium-rich natto according to claim 1, it is characterised in that natto withered grass bar in step 2 The inoculum concentration of bacterium is 1:500-700.
  5. 5. a kind of method for preparing selenium-rich natto according to claim 1, it is characterised in that be catalyzed described in step 3 mixed Close the weight ratio of mixture, wherein Organic Selenium, citric acid and deionized water that liquid is made up of Organic Selenium, citric acid and deionized water For 1:2-3:1-4.
  6. A kind of 6. method for preparing selenium-rich natto according to claim 1, it is characterised in that plasma treatment in step 3 Frequency be 1200-1500HZ, the temperature of deepfreeze is 10-15 DEG C.
  7. A kind of 7. method for preparing selenium-rich natto according to claim 1, it is characterised in that oxygen in fermentation tank in step 4 Gas content maintains 80-85%.
CN201710810024.7A 2017-09-11 2017-09-11 A kind of method for preparing selenium-rich natto Pending CN107495079A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109170813A (en) * 2018-09-06 2019-01-11 南昌大学 A kind of production method of high peppery property selenium-rich capsicum garlic paste

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101411437A (en) * 2007-10-18 2009-04-22 北京小西保健食品有限公司 Technique for preparing natto and health care food containing natto extract
CN106820127A (en) * 2017-03-11 2017-06-13 北京中科卓尔生物科技有限公司 Selenium-rich natto fine powder and preparation method thereof
CN106916810A (en) * 2017-05-04 2017-07-04 南京工业大学 One kind rapidly and efficiently screens farnoquinone(MK‑7)The method of Producing Strain
CN106942608A (en) * 2017-03-11 2017-07-14 北京中科卓尔生物科技有限公司 Natto fine powder and preparation method thereof
CN106954797A (en) * 2017-03-11 2017-07-18 北京中科卓尔生物科技有限公司 Polypeptide natto powder and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101411437A (en) * 2007-10-18 2009-04-22 北京小西保健食品有限公司 Technique for preparing natto and health care food containing natto extract
CN106820127A (en) * 2017-03-11 2017-06-13 北京中科卓尔生物科技有限公司 Selenium-rich natto fine powder and preparation method thereof
CN106942608A (en) * 2017-03-11 2017-07-14 北京中科卓尔生物科技有限公司 Natto fine powder and preparation method thereof
CN106954797A (en) * 2017-03-11 2017-07-18 北京中科卓尔生物科技有限公司 Polypeptide natto powder and preparation method thereof
CN106916810A (en) * 2017-05-04 2017-07-04 南京工业大学 One kind rapidly and efficiently screens farnoquinone(MK‑7)The method of Producing Strain

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109170813A (en) * 2018-09-06 2019-01-11 南昌大学 A kind of production method of high peppery property selenium-rich capsicum garlic paste

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Application publication date: 20171222