CN106954797A - Polypeptide natto powder and preparation method thereof - Google Patents
Polypeptide natto powder and preparation method thereof Download PDFInfo
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- CN106954797A CN106954797A CN201710143303.2A CN201710143303A CN106954797A CN 106954797 A CN106954797 A CN 106954797A CN 201710143303 A CN201710143303 A CN 201710143303A CN 106954797 A CN106954797 A CN 106954797A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of polypeptide natto powder, including following components represented with parts by weight：30 40 parts of selenium-rich natto powder, 10 parts of fruit powder, 46 parts of soyabean polypeptide powder, 9 12 parts of the clear polypeptide powder of ovum gallinaceum, 47 parts of whey polypeptide powder, 12 14 parts of konjaku flour.The method for making the polypeptide natto powder is：Selenium-rich soya bean impregnated in water after boiling 22 30 minutes, 40 DEG C are cooled to after boiling, bafillus natto after fermentation is inoculated with by 1% 5% inoculum concentration, then it is placed in 0 DEG C to place 12 hours, natto is made, natto is freeze-dried to remove moisture removal, crushed with pulverizer preliminary crushing to 60 mesh, then with ultramicro grinding homogenizer, cross 180 mesh vibratory sieves and natto powder is made, by selenium-rich natto powder, fruit powder, soyabean polypeptide powder, the clear polypeptide powder of ovum gallinaceum, whey polypeptide powder, polypeptide natto powder is made after konjaku flour mixing.The polypeptide natto powder is capable of the recurrence of thrombus always while have a good thrombolytic effect to a certain extent.
Description
Technical field
The present invention relates to healthy food manufacture field, in particular, it is related to a kind of polypeptide natto powder and its preparation side
Method.
Background technology
With the improvement of people ' s living standards, the change of dietary structure, cardiovascular and cerebrovascular disease has become the harm mankind and is good for
One of health most serious and most common disease, it is very harmful especially for the elderly.Cardiovascular and cerebrovascular disease has " morbidity
Rate height, disability rate height, death rate height, high recurrence rate, complication are more " the features such as.At present, China's Patients with Cardiovascular/Cerebrovascular Diseases has surpassed
2.7 hundred million people, China dies from the harm of embolism class diseases in nearly 3,000,000 people of cardiovascular and cerebrovascular disease, cardiovascular and cerebrovascular disease particularly every year
Seriously.
Natto is initiated by China and is popular in the traditional food of Japan, is through natto gemma bar from high-quality selenium-rich soya bean
Bacterium fermentation is formed.Natto have viscosity, not only possess the nutritive value of selenium-rich soya bean, rich in vitamin K, protein can be improved
Digestibility, it is often more important that fermentation process generates several physiological active substances, most important of which is that with thrombolysis
The Nattokinase of effect.Nattokinase is to be secreted into extracellular a kind of to have strong fibrinolytic serine egg by bafillus natto
White enzyme, can effectively dissolve important substance-insoluble fibrin of composition vascular wall patch, with powerful dissolving patch and blood
Bolt is acted on.
Eat the launch such as natto, Nattokinase, bean-containing capsule in existing market raw, this kind of product mainly according to
Dissolving patch and thrombus effect are played by the speciality of Nattokinase autolysis insoluble fibrin, and these are established
Patch and thrombus, certain damage has been had resulted in vascular wall, and after they are dissolved, the exposure of these positions being damaged,
So that the lipid in blood is easily accessible vascular wall, blood vessel is deposited on subcutaneously, the new patch of formation and thrombus are so as to cause to stop
Easily recurrence after taking.
The content of the invention
It is an object of the invention to provide a kind of polypeptide natto powder and preparation method thereof, the polypeptide natto powder has good
It is capable of the recurrence of thrombus always while thrombolytic effect to a certain extent.
To achieve the above object, the invention provides following technical scheme：
A kind of polypeptide natto powder, including following components represented with parts by weight：30-40 parts of selenium-rich natto powder, 10 parts of fruit powder, soybean
4-6 parts of polypeptide powder, clear polypeptide powder 9-12 parts of ovum gallinaceum, 4-7 parts of whey polypeptide powder, 12-14 parts of konjaku flour.
Wherein polypeptide powder can improve blood quality, safeguard cell viability, and polypeptide can be combined with many kinds of substance and transported
Various histocytes are sent to, them is preferably played a role, therefore can help to transport selenium-rich natto powder, fruit powder, konjaku flour quilt
Active principle after absorption, lifts the targeting that other component active ingredients are acted in vivo.Rich in organic in selenium-rich natto powder
Selenium, it is easy to absorption of human body, and the free radical produced after blood inner lipid is oxidized can be removed, suppress the life of internal cholesterol
Thing is synthesized, and reduces the quantity and size of the formation of patch and thrombus, so that mitigate the amount of thrombus needed for Nattokinase, fruit
Contain substantial amounts of flavonoids and pectin in powder, the metabolic adsorption of the thrombus that is dissolved by Nattokinase in vivo can be promoted.Generation
The free radical that blood inner lipid is produced after being oxidized during thanking is removed by the Organic Selenium in selenium-rich natto powder, so as to accelerate quilt
The thrombus discharge of dissolving suppresses the biosynthesis of internal cholesterol simultaneously in vivo, and one is entered while enhancing thrombolysis Scavenging activity
Step plays the effect for suppressing recurrence of thrombus.Natural mannosan and gelatinous mass in konjaku can disappear with pectin interaction
Change and layer protecting film is formed in road, and suppress absorption fatty in alimentary canal, so as to play reduction suppression cholesterol, reducing blood lipid
Effect, so as to play a part of suppressing recurrence of thrombus.
Preferably, described polypeptide natto powder, including following components represented with parts by weight：32 parts of selenium-rich natto powder,
10 parts of fruit powder, 5 parts of soyabean polypeptide powder, 10 parts of the clear polypeptide powder of ovum gallinaceum, 5 parts of whey polypeptide powder, 13 parts of konjaku flour.
A kind of method for making polypeptide natto powder, comprises the following steps：
Step 1：Selenium-rich soya bean is impregnated in water, 5-8 DEG C impregnates 20 hours；
Step 2：Selenium-rich soya bean quality 2-9% sucrose is added into selenium-rich soya bean, is subsequently placed in high steam pot, 121 DEG C
Boiling 22-30 minutes；
Step 3：Selenium-rich soya bean after boiling is cooled to 40 DEG C, 1%-5% inoculum concentration is pressed by the way of spraying, inoculation is received
Beans bacillus；
Step 4：The selenium-rich soya bean being inoculated with is promoted in fermenting cellar and fermented, fermentation temperature is 35-39 DEG C, and relative humidity is maintained at
85%-100%, fermentation can obtain fresh natto in 24 hours；
Step 5：The fresh natto fermented is placed in into 0 DEG C to place 12 hours, selenium-rich natto is made；
Step 6：The selenium-rich natto that is produced in step 5 is freeze-dried to remove moisture removal, first with pulverizer preliminary crushing to 60 mesh, then
Crushed with ultramicro grinding homogenizer, cross 180 mesh vibratory sieves and selenium-rich natto powder is made；
Step 7：Selenium-rich natto powder, fruit powder, soyabean polypeptide powder, the clear polypeptide powder of ovum gallinaceum, whey polypeptide powder, konjaku flour are placed in multidimensional
Mixed 20-30 minutes in mixer, temperature control is at 38 DEG C, then through 38 DEG C of dehumidifiers, dry, polypeptide natto powder is made.
Natto surface after being fermented wherein in step 4 forms one layer of tunica albuginea, and tacky, wire drawing is longer, gained natto color
Jaundice, there is metallic luster, and because Nattokinase is primarily present in the sticky wire drawing of natto, thus increases to a certain extent
The content of Nattokinase, and the fresh natto that ferments is placed in 0 DEG C in step 5 placed 12 hours, advantageously ensure that natto
Kinases has good activity.
Further, sodium selenite, magnesium sulfate, sodium chloride and chromium chloride are added in the water into the step 1 and battalion is made
The weight percent hundred of each component is in nutrient solution, the nutrient solution, sodium selenite 0.02%, magnesium sulfate 0.2%, sodium chloride 0.2%,
Chromium chloride 0.01%, water 95.7%.
Further, it is by richness step P1, the step P1 to be additionally provided with after the completion of the step 1, before the progress step 2
Soya bean is placed in square position selenium, then covers by the gauze of pure water-soaked, germination 10 is carried out under 28-30 DEG C, dark surrounds small
When, obtain germination selenium-rich soya bean of the sprout length in 3-5mm.
Selenium-rich soya bean fully absorbs nutrient solution after the nutrient solution immersion containing inorganic selenium, then to nothing in germination process
Machine selenium carries out sorption enhanced effect, makes Se accumulation on the amino acid contained by sprout, protein equimolecular, is received so as to increase selenium-rich
The content of Organic Selenium in beans, makes to contain selenoprotein and selenium propylhomoserin in selenium-rich natto powder, beneficial to absorption of human body, wherein selenium and albumen knot
Closing has antioxidative synergistic effects, so as to be conducive to improving the activity of Nattokinase；And in the outer of sprout bursting selenium-rich soya bean
Gap between skin, increase selenium-rich soya bean, space is provided for the fermentation of bafillus natto, and bafillus natto can lead to
Cross the gap between sprout and crust to enter in selenium-rich soya bean, the tunica albuginea for forming selenium-rich natto surface is more uniform, abundant
Be distributed in inside and outside selenium-rich natto, make that Nattokinase is distributed in natto is more uniform, and natto is added to a certain extent
The content of kinases.
Preferably, the weight ratio of the selenium-rich soya bean and nutrient solution in the step 1 is 1:4.
It is an advantage of the invention that：
1st, absorption fatty in suppression enteron aisle to a certain extent, reduction lipids contents；
2nd, internal existing thrombus and patch is dissolved；
3rd, internal free radical can be removed to a certain extent, prevents lipid oxidation, impaired blood vessel is repaired, and soften blood
Pipe increases blood vessel elasticity, prevents the formation of secondary thrombus；
4th, fat absorption, reduction lipids contents, thrombus, inhibition thrombosis, reparation blood vessel, enhancing vascular toughness etc. are suppressed
The probability of happening of many-sided collective effect, effectively reduction cardiovascular and cerebrovascular disease；
5th, fruit powder and konjaku flour give off a strong fragrance, sweet flavor, it is slightly sour can effectively remove the ammonia stink of natto powder, enhancing product mouthful
Sense；
6th, product of the present invention is when brewing for 40 DEG C, gelatinous mass and glucomannans contained by konjaku flour, can water absorption and swelling formation glue
Body parcel natto powder, polypeptide powder and fruit powder, can reduce the destruction to Nattokinase and other active materials such as hydrochloric acid in gastric juice, activity into
Divide not easy in inactivation；
7th, while will be remained in inside and outside selenium-rich soya bean rich in vegetable active selenium and Organic Selenium, and while Nattokinase is produced
Inorganic selenium changes into Organic Selenium, greatly reduces the residual of inorganic selenium, is easy to absorption of human body.
Brief description of the drawings
Fig. 1 is the fabrication processing figure in the present invention.
Embodiment
Below by embodiment, the invention will be further described.It should be understood that being prepared described in the embodiment of the present invention
Method is only used for the explanation present invention, rather than limitation of the present invention, to present invention system under the concept thereof of the present invention
The simple modifications of Preparation Method belong to the scope of protection of present invention.
Involved all substances are commercially available in the embodiment of the present invention, and soyabean polypeptide powder is purchased from Tianjin day biological section all one's life
Skill Co., Ltd, the clear polypeptide powder of ovum gallinaceum are sub- permanent purchased from Shandong purchased from Xi'an asparagus fern bio tech ltd part, whey polypeptide powder
Bio tech ltd；Xi'an Bei La bio tech ltd is purchased from as fruit powder apple powder, peach powder, pears powder；Konjaku
Powder is purchased from Henan Yong En bio tech ltd.
Embodiment 1：
The manufacturing process of polypeptide natto powder is as follows：
A, production natto
1. selenium-rich soya bean pre-processes：By raw material selenium-rich soya bean by manual grading skill screen, remove selenium-rich soya bean in mix stone,
The impurity such as wood chip, through hand picking full seed, size is unified, (selenium-rich soya bean selects Hubei grace to bright selenium-rich soya bean
Apply the selenium-rich soya bean of plantation)；
2. dipping：By weight 1 after the selenium-rich soya bean of pretreatment is cleaned:4 ratio, which is added in nutrient solution, to be impregnated, 7 DEG C of dippings
20 hours, rinsed afterwards with pure water, wherein nutrient solution includes following component as expressed in weight percent：Sodium selenite
0.02%th, magnesium sulfate 0.2%, sodium chloride 0.2%, chromium chloride 0.01%, pure water 95.7%；
3. germination：The selenium-rich soya bean impregnated is placed in square position, then covered by the gauze of pure water-soaked, 28-30 DEG C,
Germination 10 hours is carried out under dark surrounds, germination selenium-rich soya bean of the sprout length in 3-5mm is obtained；
4. boiling：The selenium-rich soya bean germinateed is placed in high steam pot, 121 DEG C of boilings 25 minutes；
5. inoculation：Selenium-rich soya bean after boiling is cooled to 40 DEG C or so, 3% inoculum concentration, inoculation are pressed by the way of spraying
Bafillus natto；
6. fermentation：The selenium-rich soya bean being inoculated with is promoted in fermenting cellar and fermented, fermentation temperature is 37 DEG C, and relative humidity is maintained at
Between 85%-100%, fermentation can obtain fresh natto in 24 hours；
7. maturation：The natto fermented is placed in into 0 DEG C to place 12 hours, selenium-rich natto is made.
B, prepare polypeptide natto powder
1. removing moisture removal by the selenium-rich natto produced in step A is freeze-dried, first with pulverizer preliminary crushing to 60 mesh, then use
Ultramicro grinding homogenizer is crushed, and crosses 180 mesh vibratory sieves and selenium-rich natto powder is made；
2. by selenium-rich natto powder 320g, soyabean polypeptide powder 50g, the clear polypeptide powder 100 of ovum gallinaceum, whey polypeptide powder 50, apple powder 50g,
Peach powder 20g, pears powder 30g, konjaku flour 130g are placed in Multidimensionblender and mixed 20-30 minutes, and temperature control is in 38 DEG C, Ran Houjing
38 DEG C of dehumidifiers, dry, polypeptide natto powder are made.
Embodiment 2-7 is sucrose addition, the time of boiling, the temperature of fermentation, inoculum concentration with the difference of embodiment 1
Difference, and the proportioning of the clear polypeptide powder of selenium-rich natto powder, soyabean polypeptide powder, ovum gallinaceum, whey polypeptide powder, konjaku flour are different, as follows
Shown in table 1：
Table 1
Embodiment 8：The difference of embodiment 1 is not add sucrose in digestion process.
The natto sensory properties that embodiment 1 to 8 is produced：
The natto of embodiment 1-8 technique productions, the natto surface after fermentation forms one layer of tunica albuginea, and tacky, wire drawing is longer, gained
Natto yellowish, there is metallic luster.The natto viscosity of the wherein production technology production of embodiment 1 is most strong, and wire drawing is most long, and by
It is primarily present in the sticky wire drawing of natto, therefore can tentatively finds out from the sensory properties of natto in Nattokinase, implements
There is higher Nattokinase content in the natto that example 1 is produced.
Given birth to bacterium colony colony counting method and FU determined by ultraviolet spectrophotometry embodiment 1 to the fermentation selenium-rich soya bean of embodiment 8
The content of bafillus natto and Nattokinase in the natto of production, as shown in table 2：
Table 2
Authorization Notice No. is CN101411437B Chinese patent, uses dip time for 20-27min, after boiling 20-25min,
The natto of 30-55 DEG C of fermentation 10-16h production technology production, natto specification is Nattokinase content >=230FU, natto gemma
Bacillus number >=3*109cfu/g。
1000FU is above by the Nattokinase content produced in embodiment 1-8 as can be seen from Table 2, with prior art
Increased significantly compared to tool, wherein and wherein the content of bafillus natto and Nattokinase is higher in embodiment 1, natto bud
Spore bacillus content, which increases, is conducive to it to secrete more Nattokinases, and the Nattokinase of high level is conducive to dissolving composition blood
Important substance-insoluble fibrin of tube wall patch, and then increase dissolving patch and thrombus effect.
As can be seen from the above table, without sucrose fermentation selenium-rich soya bean, the Nattokinase and natto bud of the natto produced
Spore bacillus number will be because while that the carbon-nitrogen ratio of soybean in itself is about 5 less than the fermentation method for adding sucrose:1, it can meet
The basic growth demand of bafillus natto, but it is a certain degree of improve bafillus natto growing environment carbon-nitrogen ratio be conducive to
The growth of bafillus natto, and then more Nattokinases can be also produced in growth course, but shown from test data, when
Sucrose addition is more than after 5%, and the value added on products of bafillus natto tends towards stability, and bafillus natto number does not substantially increase
Plus.
Experimental example A:Polypeptide natto powder sensory properties
It is random to select 16-30 Sui, 30-55 Sui, the subject of 50-80 Sui three age brackets in the present invention each embodiment preparation it is many
Peptide natto powder and commercially available pure selenium-rich natto powder product, fresh natto product carry out sensory aspect (color and luster, smell, mouthfeel) evaluation and beaten
Point, appraisal result is as shown in table 3 below, wherein each age bracket selects 30 subjects, take altogether 90 subjects score number
Average value is final result.In test, color and luster：10 points are the color liked the most of subject；Smell：10 points are liked for subject
Highest scoring；Taste：10 points of highests liked for subject score.
Table 3
As a result show, polypeptide natto powder prepared by embodiment 1-7 is substantially better than pure selenium-rich natto powder and fresh on smell and mouthfeel
Natto, obtains the consistent accreditation of subject.
Experimental example B：Effect to thrombus in rat body
Thrombus experiment, anti-oxidant experiment in rat body, the experiment of natto application effect, the micro- blood of prevention to illustrate the present composition
Bolt and oxidation resistant effect.
1st, experiment material
1.1 experimental drugs
1.2 experimental animals：Rat is purchased from Beijing magnificent Co., Ltd of dimension tonneau, male.
1.3 laboratory apparatus：ACL coagulo meters
1.4 experiment reagents：TT (thrombin time of blood plasma measure kit), PT (serum PT in patients kit),
APTT (active partial thromboplastin time measure kit), FIB (fibrinogen assay kit) reagent must purchased from Nanjing
Shi get biotechnologies company.
2nd, experimental method
By 90 healthy rats, every 10 one group is randomly divided into 9 groups, and each group uses gavage approach daily in addition to normal feeding
It is administered once, gavage 30 days.
Negative control group：Daily group gives physiological saline gavage once, daily according to 10ml/kg body weight gavages, without modeling
Processing；
Model control group：Daily group gives physiological saline gavage once, daily according to 10ml/kg body weight gavages, the 3rd day gavage reciprocal
After start modeling processing.
The polypeptide natto powder prepared from embodiment 1 is as tested material, and the human body recommended dose of the tested material is 0.05g/
D*kg body weight.10 times of the dose,equivalent of rat equivalent to human body recommended dose.Respectively with 5 times of human body recommended dose, 10 times
With 30 times as basic, normal, high dosage, separately constitute in polypeptide natto powder low dose group (abbreviation low dose group), polypeptide natto powder
Dosage group (abbreviation middle dose group), polypeptide natto powder high dose group (abbreviation high dose group).By the tested material of basic, normal, high dosage,
Brewed with pure water, daily according to 10ml/kg body weight gavages.Start modeling processing after 3rd day gavage reciprocal.
Composition A groups：The fruit powder of apple powder, peach powder and pears powder composition, test dose are lacked compared with polypeptide natto powder group
It is identical with middle dose group, start modeling processing after the 3rd day gavage reciprocal.
Composition B groups：Soyabean polypeptide powder, the clear polypeptide powder of ovum gallinaceum, whey polypeptide powder group are lacked compared with polypeptide natto powder group
Into polypeptide powder, test dose is identical with middle dose group, starts modeling processing after the 3rd day gavage reciprocal.
Composition C groups：Selenium-rich natto powder is lacked compared with polypeptide natto powder group, test dose is identical with middle dose group, fallen
Start modeling processing after the 3rd day gavage of number.
Composition D groups：Konjaku flour is lacked compared with polypeptide natto powder group, test dose is identical with middle dose group, inverse the 3rd
Start modeling processing after its gavage.
Start modeling after gavage within the 3rd day in inverse, precision weighs carrageenan, and 3.5% (g/ml) is made into physiological saline
Concentration；To rat weight, in addition to negative control group, carrageenan is subcutaneously injected in metapedes toe in each group, and dosage is 30mg/kg
Body weight.Rat is placed in black tail length after (18 ± 2) DEG C indoor feeding, measurement rat modeling 24h, 48h, 72h after modeling.Most
Afterwards after one-shot measurement black tail length, each group rat anesthesia, inferior caval vein takes whole blood, is sub-packed in two test tubes, takes whole blood, determines solidifying
Hemase time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB).
3rd, statistical method, continuous data represents that comparison in difference is examined using t between each group with average value ± standard error, by
Excel software statistics are completed.P ＜ 0.05 is there were significant differences, and p ＜ 0.01 are to have pole significant difference.
4th, result and analysis, influence of the polypeptide natto powder to rat tail and such as table 4 below of the influence to blood coagulation system and
Shown in table 5.
Effect of the different product of table 4 to rat black tail length (cm)
Note：Compared with negative control group, * * P ＜ 0.01, * * * P ＜ 0.001；Compared #P ＜ 0.05, ##P ＜ with model group
0.01, ###P ＜ 0.001.
As shown in Table 4：Compared with negative control group, model group 48h black tails length extremely significantly increases (P ＜ 0.001)；
At 24 hours, composition A groups substantially suppressed the growth (P ＜ 0.05) of black tail compared with model group, and low dose group is notable
Suppression black tail growth (P ＜ 0.01), middle dose group and high dose group pole significantly inhibit the growth (P ＜ 0.001) of black tail；With
Composition A groups, composition B groups, composition C groups, the effect of composition D groups are compared, and basic, normal, high dosage group is respectively provided with preferably
Effect, and the effect of composition A groups, composition B groups, composition C groups, composition D groups is significantly lower than low dose group.
At 48 hours, low dose group can significantly suppress the growth (P ＜ 0.01) of black tail, middle dose group, high dose group
It extremely can significantly suppress the growth (P ＜ 0.001) of black tail；With composition A groups, composition B groups, composition C groups, composition D groups
Effect compare, the effect of excellent three groups of the effect of basic, normal, high dosage group, and composition A groups, composition B groups, composition C
Group, the effect of composition D groups are significantly lower than low dose group.
At 72 hours, composition A groups substantially suppressed the growth (P ＜ 0.05) of black tail, and low dose group significantly suppresses black
The growth (P ＜ 0.01) of tail, middle dose group and high dose group pole significantly inhibit the growth (P ＜ 0.001) of black tail；With composition A
Group, composition B groups, composition C groups, the effect of composition D groups are compared, and basic, normal, high dosage group is respectively provided with preferably effect, and
Composition A groups, composition B groups, composition C groups, the effect of composition D groups are significantly lower than low dose group.
Influence of the table 5 to blood coagulation system
Note：Compared with negative control group, * * P ＜ 0.01, * * * P ＜ 0.001；Compared #P ＜ 0.05, ##P ＜ with model group
0.01, ###P ＜ 0.001.
As shown in Table 5, compared with negative control group, the thrombin time of model group significantly shortens (P ＜ 0.01), fiber egg
White former increase, but without significant difference；Compared with model group, composition A groups, composition B groups, composition C groups, composition D groups
Prothrombin time (P ＜ 0.05) can significantly be increased, low dose group, middle dose group can significantly increase prothrombin time (P
＜ 0.01), high dose group extremely can significantly increase thrombin time (P ＜ 0.001)；With composition A groups, composition B groups, combine
Thing C groups, the effect of composition D groups are compared, and the effect of basic, normal, high dosage group is substantially better than the effect.
Binding analysis table 4 and table 5：
Composition A groups lack the fruit powder of apple powder, peach powder and pears powder composition, and contain substantial amounts of flavonoids and pectin in fruit powder,
The metabolic adsorption of the thrombus that is dissolved by Nattokinase in vivo can be promoted.Produced after blood inner lipid is oxidized in metabolic process
Raw free radical is removed by the Organic Selenium in selenium-rich natto powder, so as to accelerate dissolved thrombus discharge to suppress body simultaneously in vivo
The biosynthesis of inner cholesterol, the effect for suppressing recurrence of thrombus is further functioned as while enhancing thrombolysis Scavenging activity.And
And the free radical that produces reduces its damage to vascular endothelial cell after being eliminated after being oxidized, so as to aid in increasing brass reparation
The efficiency of the impaired blood vessel exposed after being dissolved because of thrombus by Nattokinase, softening blood vessel increase blood vessel elasticity, reduces blood fat
In the probability of impaired place's accumulation rows of thrombus of shape.
Lack the clear polypeptide powder of soyabean polypeptide powder, ovum gallinaceum, the polypeptide powder of whey polypeptide powder composition, and polypeptide in composition B groups
Powder can improve blood quality, safeguard cell viability, and polypeptide can combine with many kinds of substance and be conveyed to various histocytes,
Them is preferably played a role, therefore can help to transport the active principle after selenium-rich natto powder, fruit powder, konjaku flour are absorbed,
Lift the targeting that other component active ingredients are acted in vivo.In addition, polypeptide powder, which also has, safeguards that gastrolintestinal microecology is balanced, carry
High fitness, defending and fighting against diseases promote rehabilitation, body immunity is in optimum state.And wherein soya-bean polypeptides have
The effects such as antithrombus formation, suppression platelet aggregation, reducing blood lipid and cholesterol, other component anti-thrombogenic capacities can be strengthened.
Lack selenium-rich natto powder in composition C groups, and Nattokinase is the main matter of thrombus in natto, so that molten
Thrombolysis efficiency is reduced on bolt source, and only provides reduction lipids contents by tomato meal, fruit powder and konjaku flour, suppress low density lipoprotein
The effects such as the impaired blood vessel of protein oxidation, reparation, it, which drops thrombus effect, to substantially reduce.In addition, the selenium in selenium-rich natto powder is
Food source antioxidant, can remove the excessive oxygen radical of human body, can prevent the free radical that lipid is produced after being oxidized to blood vessel
The damage of endothelial cell, whole composition can be caused by lacking Organic Selenium, and the function of repairing improvement blood vessel is had a greatly reduced quality, so as to weaken
The function of pre- preventing thrombosis.
Lack konjaku flour in composition D groups, gelatinous mass and glucomannans contained by konjaku flour can water absorption and swelling formation glue
Body parcel selenium-rich natto powder, pineapple powder and apple powder, can reduce hydrochloric acid in gastric juice etc. to Nattokinase, other protease and remove freely
The destruction of the active material of base, it is ensured that active component non-inactivation.In addition, glucomannans and gelatinous mass in konjaku can be with
Synergy, suppresses absorption fatty in alimentary canal.Lack protective effect of the konjaku flour to other Composition Active materials, can cause
Active ingredient partial inactivation in other components, reduction thrombus, the work(for repairing blood vessel, reduction blood fat and inhibition thrombosis
Effect.
Equally, embodiments of the invention 2-7 formula is tested, effect is same as Example 1, can suppresses black tail
Length, increase factor time, with synergy.
Experimental example C：
Choose the polypeptide natto powder for preparing in embodiment 1, according to daily warm boiled water 1-2g, D1, D2, D3, D4, D5 five by
Examination person is taken four months, and the arteria carotis of the patient is detected using colorful Doppler ultrasound diagnostic apparatus, predominantly detects the blood of subject
Patch, endangium thickness and blood flow state in pipe.Take front and rear physical examination result as shown in table 6 below.
Table 6
The physical examination result of each patient shows that the blood vessel thickness for taking the patient after four months is obviously reduced, vascular wall
Caliper recovery is normal.Blood drag index declines, frequency spectrum compared with four months before when not taking, more regular, overall blood flow substantially changes
It is kind.In addition, the original cardiovascular and cerebrovascular abnormality of patient is considerably reduced, especially numbness in hands and feet, asthma uncomfortable in chest, brain activity be not
The cardiovascular and cerebrovascular abnormalities such as foot slow down significantly.
Claims (6)
1. a kind of polypeptide natto powder, it is characterised in that including following components represented with parts by weight：
30-40 parts of selenium-rich natto powder,
10 parts of fruit powder,
4-6 parts of soyabean polypeptide powder,
Clear polypeptide powder 9-12 parts of ovum gallinaceum,
4-7 parts of whey polypeptide powder,
12-14 parts of konjaku flour.
2. polypeptide natto powder according to claim 1, it is characterised in that including following components represented with parts by weight：
32 parts of selenium-rich natto powder,
10 parts of fruit powder,
5 parts of soyabean polypeptide powder,
10 parts of the clear polypeptide powder of ovum gallinaceum,
5 parts of whey polypeptide powder,
13 parts of konjaku flour.
3. a kind of method for preparing the polypeptide natto powder described in claim 1-2 any one, it is characterised in that including following step
Suddenly：
Step 1：Selenium-rich soya bean is impregnated in water, 5-8 DEG C impregnates 20 hours；
Step 2：Selenium-rich soya bean quality 2-9% sucrose is added into selenium-rich soya bean, is subsequently placed in high steam pot, 121 DEG C
Boiling 22-30 minutes；
Step 3：Selenium-rich soya bean after boiling is cooled to 40 DEG C, 1%-5% inoculum concentration is pressed by the way of spraying, inoculation is received
Beans bacillus；
Step 4：The selenium-rich soya bean being inoculated with is promoted in fermenting cellar and fermented, fermentation temperature is 35-39 DEG C, and relative humidity is maintained at
85%-100%, fermentation can obtain fresh natto in 24 hours；
Step 5：The fresh natto fermented is placed in into 0 DEG C to place 12 hours, selenium-rich natto is made；
Step 6：The selenium-rich natto that is produced in step 5 is freeze-dried to remove moisture removal, first with pulverizer preliminary crushing to 60 mesh, then
Crushed with ultramicro grinding homogenizer, cross 180 mesh vibratory sieves and selenium-rich natto powder is made；
Step 7：Selenium-rich natto powder, fruit powder, soyabean polypeptide powder, the clear polypeptide powder of ovum gallinaceum, whey polypeptide powder, konjaku flour are placed in multidimensional
Mixed 20-30 minutes in mixer, temperature control is at 38 DEG C, then through 38 DEG C of dehumidifiers, dry, polypeptide natto powder is made.
4. the preparation method of polypeptide natto powder according to claim 3, it is characterised in that：In water into the step 1
Add the weight percent hundred that each component in nutrient solution, the nutrient solution is made in sodium selenite, magnesium sulfate, sodium chloride and chromium chloride
For sodium selenite 0.02%, magnesium sulfate 0.2%, sodium chloride 0.2%, chromium chloride 0.01%, water 95.7%.
5. the preparation method of polypeptide natto powder according to claim 4, it is characterised in that：After the completion of the step 1, carry out
Step P1, the step P1 are additionally provided with before the step 2 for selenium-rich soya bean is placed in square position, is then covered by pure water-soaked
Gauze, under 28-30 DEG C, dark surrounds carry out germination 10 hours, obtain germination selenium-rich soya bean of the sprout length in 3-5mm.
6. the preparation method of polypeptide natto powder according to claim 5, it is characterised in that：Selenium-rich in the step 1 is yellow
The weight ratio of beans and nutrient solution is 1:4.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107495079A (en) * | 2017-09-11 | 2017-12-22 | 南京御匾国健生物科技有限公司 | A kind of method for preparing selenium-rich natto |
| CN109182312A (en) * | 2018-09-25 | 2019-01-11 | 武汉黄家医圈生物医药有限公司 | A kind of method and application thereof of composite microbial Tetramune high efficient expression |
| CN109965219A (en) * | 2019-03-22 | 2019-07-05 | 广州金酮特殊医用食品有限公司 | A kind of preparation method of natto powder and whitening spot-removing alimentation composition containing natto powder |
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