CN107494705A - A kind of method using the fresh-keeping fruit of algal polysaccharides - Google Patents
A kind of method using the fresh-keeping fruit of algal polysaccharides Download PDFInfo
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- CN107494705A CN107494705A CN201710698332.5A CN201710698332A CN107494705A CN 107494705 A CN107494705 A CN 107494705A CN 201710698332 A CN201710698332 A CN 201710698332A CN 107494705 A CN107494705 A CN 107494705A
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- algal polysaccharides
- fresh
- antistaling agent
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- polysaccharides
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- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 72
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 45
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 26
- 150000004676 glycans Chemical class 0.000 title claims abstract 21
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 24
- 238000002360 preparation method Methods 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 8
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 27
- 239000000706 filtrate Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 17
- 239000000284 extract Substances 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 12
- 239000003960 organic solvent Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 241000219991 Lythraceae Species 0.000 claims description 7
- 235000014360 Punica granatum Nutrition 0.000 claims description 7
- 244000269722 Thea sinensis Species 0.000 claims description 7
- 229940069521 aloe extract Drugs 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 7
- 235000013824 polyphenols Nutrition 0.000 claims description 7
- 241000283690 Bos taurus Species 0.000 claims description 6
- 238000009938 salting Methods 0.000 claims description 6
- 229930002875 chlorophyll Natural products 0.000 claims description 5
- 235000019804 chlorophyll Nutrition 0.000 claims description 5
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 5
- 238000002386 leaching Methods 0.000 claims description 5
- 238000000967 suction filtration Methods 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 3
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 3
- 241001474374 Blennius Species 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 claims description 3
- 241000206754 Palmaria palmata Species 0.000 claims description 3
- 241000195474 Sargassum Species 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000002425 crystallisation Methods 0.000 claims description 3
- 230000008025 crystallization Effects 0.000 claims description 3
- 239000001921 dulse Substances 0.000 claims description 3
- 229940082787 spirulina Drugs 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 9
- 238000004321 preservation Methods 0.000 abstract description 7
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract description 2
- 150000004804 polysaccharides Chemical class 0.000 description 51
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 238000010612 desalination reaction Methods 0.000 description 4
- -1 bromine phenolic compound Chemical class 0.000 description 3
- DGLAHESNTJWGDO-SRVKXCTJSA-N His-Ser-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)N DGLAHESNTJWGDO-SRVKXCTJSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 108010040030 histidinoalanine Proteins 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 150000005208 1,4-dihydroxybenzenes Chemical class 0.000 description 1
- MXNRLFUSFKVQSK-UHFFFAOYSA-N 2-Amino-6-(trimethylazaniumyl)hexanoate Chemical compound C[N+](C)(C)CCCCC(N)C([O-])=O MXNRLFUSFKVQSK-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- DECCMEWNXSNSDO-ZLUOBGJFSA-N Ala-Cys-Ala Chemical compound C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(O)=O DECCMEWNXSNSDO-ZLUOBGJFSA-N 0.000 description 1
- OBVSBEYOMDWLRJ-BFHQHQDPSA-N Ala-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N OBVSBEYOMDWLRJ-BFHQHQDPSA-N 0.000 description 1
- SPIPSJXLZVTXJL-ZLUOBGJFSA-N Asn-Cys-Ser Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(O)=O SPIPSJXLZVTXJL-ZLUOBGJFSA-N 0.000 description 1
- 241000206575 Chondrus crispus Species 0.000 description 1
- CVOZXIPULQQFNY-ZLUOBGJFSA-N Cys-Ala-Cys Chemical compound C[C@H](NC(=O)[C@@H](N)CS)C(=O)N[C@@H](CS)C(O)=O CVOZXIPULQQFNY-ZLUOBGJFSA-N 0.000 description 1
- XGIAHEUULGOZHH-GUBZILKMSA-N Cys-Arg-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CS)N XGIAHEUULGOZHH-GUBZILKMSA-N 0.000 description 1
- WZJLBUPPZRZNTO-CIUDSAMLSA-N Cys-Ser-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CS)N WZJLBUPPZRZNTO-CIUDSAMLSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- MHXKHKWHPNETGG-QWRGUYRKSA-N Gly-Lys-Leu Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O MHXKHKWHPNETGG-QWRGUYRKSA-N 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- WRLPVDVHNWSSCL-MELADBBJSA-N Leu-His-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N2CCC[C@@H]2C(=O)O)N WRLPVDVHNWSSCL-MELADBBJSA-N 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- DSSOYPJWSWFOLK-CIUDSAMLSA-N Ser-Cys-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(O)=O DSSOYPJWSWFOLK-CIUDSAMLSA-N 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000002366 halogen compounds Chemical class 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/08—Preserving with sugars
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a kind of method using the fresh-keeping fruit of algal polysaccharides, fresh-keeping method is to put fruits into the antistaling agent based on algal polysaccharides, taken out after standing 5 ~ 9min, naturally dry, the preservation method of the present invention, it is fresh-keeping easy to operate, it can effectively suppress the respiration of fruit, reduce scattering and disappearing for moisture and nutritional ingredient；The antistaling agent based on algal polysaccharides is used in preservation method, cost is small, and preparation process is simple, not only of good preservation effect, also high with good bactericidal action, raw material green non-pollution, sufficient raw, and preparation method implementation degree, is adapted to large-scale production.
Description
Technical field
The present invention relates to fruit freshness preserving technical field, and in particular to a kind of method using the fresh-keeping fruit of algal polysaccharides.
Background technology
Seaweed bio active material is broadly divided into two kinds, and one kind is that molecular weight is smaller, can direct or indirect shadow after absorption
Ring cylinder metabolism-ure matter, mainly including halogen compound, terpenoid, bromine phenolic compound, hydroquinones, marine alga tannin,
Laminine etc.;Another kind is the iuntercellular viscous polysaccharide for being difficult to be digested absorption, the main alginic acid included in brown alga, fucose
Agar-agar, carragheen in glue, sulfated polysaccharide, red algae etc..Polysaccharide is the important component of all living organisms, and in control cell
The organism eubolism etc. that divides, adjusts cell growth and sustain life plays an important roll.
Such as, Chinese invention patent Granted publication document, Authorization Notice No. is prior art：CN104892785B, the invention
Disclose a kind of extracting method of algal polysaccharides.This method comprises the steps of：（1）Crushed after marine alga raw material cleaning, drying,
Cross 60 mesh sieves；（2）Crushed material sieve with solid-to-liquid ratio 1:25 ~ 35 accurate addition distilled water progress ultra high pressure extractions, pressurization 200 ~
400MPa, 30 ~ 70 DEG C of temperature；Pressurize 3 ~ 8 minutes, suction filtration take filtrate, obtain algal polysaccharides crude extract；（3）Added into crude extract
Activated carbon decolorizing, filtering, mixed liquor is made；（4）Mixed liquor is further concentrated, crystallizes, filter, to be dried to obtain trehalose pure
Product.The inventive method is simple, operation is easy, extraction time is short, suitable for industrialized production, can obtain purity height, impurity is few, exempts from
The algal polysaccharides product invaded and harassed by microorganism, the decoloring ability of the extracting method activated carbon of the invention are limited, it is impossible to continuous preferable
Progress algal polysaccharides decolouring.
The content of the invention
It is an object of the invention to provide a kind of fresh-keeping easy to operate, preparation process is simple, fresh-keeping effect time limit length, fresh-keeping effect
Fruit is good, and has preferable fungistatic effect, can extend a kind of method using the fresh-keeping fruit of algal polysaccharides of fruits and vegetables shelf life.
The present invention is for the scheme taken of solution above-mentioned technical problem：A kind of side using the fresh-keeping fruit of algal polysaccharides
Method, fresh-keeping method are to put fruits into the antistaling agent based on algal polysaccharides, are taken out after standing 5 ~ 9min, naturally dry is
Can, this preservation method is simple to operate, and the injury to fruit is small, available for batch operation.
Preferably, the composition and its parts by weight of the antistaling agent based on algal polysaccharides are：15 ~ 29 parts of algal polysaccharides, pomegranate
3 ~ 6 parts of bark extract, 4 ~ 5 parts of aloe extract, 2.4 ~ 3.6 parts of tea polyphenol extract thing, 40 ~ 60 parts of sterilized water, this antistaling agent are former
Expect green non-pollution, and sufficient raw, prepared antistaling agent have good preserving and sterilizing effect.
Preferably, algal polysaccharides by one kind in spirulina, seaweed, sargassum, dulse, green alga, loose algae or
A variety of extractions.
Preferably, the antistaling agent preparation method based on algal polysaccharides comprises the following steps：
1）Marine alga raw material to be cleaned, marine alga is extracted using sonioation method, suction filtration takes filtrate, obtains the leaching liquor containing algal polysaccharides,
Standby, high using sonioation method extraction efficiency, extraction time is short；
2）Gained filtrate in step 1 is taken, organic solvent is added, is stood after stirring, wait to be layered, removes upper strata part containing chlorophyll layer
Liquid, it is standby, organic solvent is added, the layered velocity of filtrate can be effectively improved, and layered effect is improved, improve follow-up obtained sea
The quality of polysaccharides；
3）Filtrate in step 2 is taken, carries out de-salting operation, standby, the filtrate scope of application after desalination is wider so that algal polysaccharides
Purity is higher；
4）Take liquid in step 3 concentrated, crystallized, filtered, dried, obtain algal polysaccharides sterling；
5）By weight, the algal polysaccharides in step 4 are taken, add pomegranate rind extract, aloe extract, tea polyphenol extract
The antistaling agent based on algal polysaccharides is made after stirring in thing, sterilized water.
Preferably, sonioation method uses Ultrasonic Cell Disruptor in preparation process 1, ultrasonic frequency is 21 ~ 24KHz, leaching
It is 15 ~ 23min to carry the time, and the control of ultrasonic frequency causes the stability of molecule that will not only injure algal polysaccharides, can also carried
The recovery rate of high algal polysaccharides.
Preferably, the organic solvent added in preparation process 2 is 0.2 ~ 0.5 times of volume of filtrate, Elements in Organic Solvents
For：Ethyl acetate and active peptides, the ratio of weight successively of composition is 1:0.2 ~ 0.4, the amino acid sequence of active peptides is：
HSHACACSKHSHAGTLSCLCRVLHPGKLCACVNCSR, active peptides are combined with the active site of ethyl acetate, effectively
The poisonous effect of ethyl acetate is reduced, only liquid layered does not provide favorable environment, can also avoid toxicant residual pair
The use of later stage algal polysaccharides brings influence.
Preferably, de-salting operation uses ionodialysis method in preparation process 3, the reaction condition of ionodialysis method is gentle,
Reaction time is short, and desalting effect is good, can effectively improve the quality of algal polysaccharides.
Preferably, in preparation process 4, concentrate and be concentrated under reduced pressure using Rotary Evaporators, liquid concentration to water content be 68 ~
77%, crystallization operation is the ethanol that the concentration of 3 ~ 6 times of volumes of addition is 90 ~ 96%, and polysaccharide does not dissolve in high concentration ethanol, liquid concentration
The addition of ethanol, not only material saving can be reduced afterwards, and make it that amount of ions is more in unit volume, and crystalline rate is high.
Compared with prior art, beneficial effects of the present invention are:The preservation method of the present invention, it is fresh-keeping easy to operate, can have
The respiration of effect suppression fruit, reduction moisture and nutritional ingredient are scattered and disappeared；The guarantor based on algal polysaccharides is used in preservation method
Fresh dose, cost is small, and preparation process is simple, not only of good preservation effect, also with good bactericidal action, raw material green non-pollution,
Sufficient raw, and preparation method implementation degree is high, is adapted to large-scale production；It is more that activity is with the addition of in antistaling agent preparation process
Peptide, the toxicity of ethyl acetate is effectively reduced, make manufactured antistaling agent property stable, purity height is nontoxic, has well
Application prospect.
Embodiment
It is described in further detail with reference to embodiments：
Embodiment 1：
A kind of method using the fresh-keeping fruit of algal polysaccharides, fresh-keeping method are to put fruits into the antistaling agent based on algal polysaccharides
In, taken out after standing 5 ~ 9min, naturally dry.
The composition and its parts by weight of antistaling agent based on algal polysaccharides be：22 parts of algal polysaccharides, 5 parts of pomegranate rind extract,
4.7 parts of aloe extract, 2.9 parts of tea polyphenol extract thing, 44 parts of sterilized water, this antistaling agent raw material green non-pollution, and raw material come
Source is sufficient, and prepared antistaling agent has good preserving and sterilizing effect.
Algal polysaccharides in spirulina, green alga, loose algae by extracting.
Antistaling agent preparation method based on algal polysaccharides comprises the following steps：
1）Marine alga raw material to be cleaned, marine alga is extracted using sonioation method, suction filtration takes filtrate, obtains the leaching liquor containing algal polysaccharides,
Standby, high using sonioation method extraction efficiency, extraction time is short；
2）Gained filtrate in step 1 is taken, organic solvent is added, is stood after stirring, wait to be layered, removes upper strata part containing chlorophyll layer
Liquid, it is standby, organic solvent is added, the layered velocity of filtrate can be effectively improved, and layered effect is improved, improve follow-up obtained sea
The quality of polysaccharides；
3）Filtrate in step 2 is taken, carries out de-salting operation, standby, the filtrate scope of application after desalination is wider so that algal polysaccharides
Purity is higher；
4）Take liquid in step 3 concentrated, crystallized, filtered, dried, obtain algal polysaccharides sterling；
5）By weight, the algal polysaccharides in step 4 are taken, add pomegranate rind extract, aloe extract, tea polyphenol extract
The antistaling agent based on algal polysaccharides is made after stirring in thing, sterilized water.
Sonioation method uses Ultrasonic Cell Disruptor, ultrasonic frequency 24KHz in preparation process 1, and extraction time is
18min, the control of ultrasonic frequency cause the stability of molecule that will not only injure algal polysaccharides, can also improve algal polysaccharides
Recovery rate.
The organic solvent added in preparation process 2 is 0.27 times of volume of filtrate, and Elements in Organic Solvents is：Ethyl acetate and
Active peptides, the ratio of weight successively of composition is 1:0.31, the amino acid sequence of active peptides is：
HSHACACSKHSHAGTLSCLCRVLHPGKLCACVNCSR, active peptides are combined with the active site of ethyl acetate, effectively
The poisonous effect of ethyl acetate is reduced, only liquid layered does not provide favorable environment, can also avoid toxicant residual pair
The use of later stage algal polysaccharides brings influence.
De-salting operation uses ionodialysis method in preparation process 3.
In preparation process 4, concentrate and be concentrated under reduced pressure using Rotary Evaporators, liquid concentration to water content is 72%, crystallization operation
To add the ethanol that the concentration of 3.8 times of volumes is 95%.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
State.
Embodiment 2：
A kind of method using the fresh-keeping fruit of algal polysaccharides, fresh-keeping method are to put fruits into the antistaling agent based on algal polysaccharides
In, taken out after standing 5 ~ 9min, naturally dry, wherein the preparation method of the antistaling agent based on algal polysaccharides is as follows：By weight
Part meter is measured, takes 22 parts of sargassum, dulse, green alga mixture cut-outs to clean, using Ultrasonic Cell Disruptor, controls ultrasonic wave frequency
Rate is 22KHz, extraction time 16min, carries out marine alga extraction, is filtered after the completion of extraction and takes filtrate, is made containing algal polysaccharides
Leaching liquor；0.3 times of volume is added into filtrate contains ethyl acetate and active peptides, and weight ratio is followed successively by 1:0.25:0.64
Organic solvent, stand 1h after stirring 3min with homogenizer, remove liquid of the upper strata containing chlorophyll；After chlorophyll is removed
With ionodialysis method desalination, the liquid after desalination is concentrated under reduced pressure liquid using Rotary Evaporators, and liquid concentration to water content is
70%, afterwards add 3.5 times of volumes concentration be 93% ethanol stir, it is to be crystallized completely after, filtering, dry, be made algal polysaccharides
Sterling, by algal polysaccharides and 2.8 parts of 4 parts of pomegranate rind extract, 4.3 parts of aloe extract, tea polyphenol extract thing mixing, add 44
Part sterilized water dissolving, stirs and the antistaling agent based on algal polysaccharides is made.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
State.
Specific embodiment described herein is only to present invention explanation for example.The technical field of the invention
Technical staff can be made various modifications or supplement to described specific embodiment or be substituted using similar mode, but
Without departing from the present invention's or surmount scope defined in appended claims.
SEQUENCE LISTING
<110>Lanxi City silence bio tech ltd
<120>A kind of method using the fresh-keeping fruit of algal polysaccharides
<130> 2
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 36
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Cys Ser Lys His Ser His Ala Gly Thr Leu
1 5 10 15
Ser Cys Leu Cys Arg Val Leu His Pro Gly Lys Leu Cys Ala Cys Val
20 25 30
Asn Cys Ser Arg
35
Claims (8)
- A kind of 1. method using the fresh-keeping fruit of algal polysaccharides, it is characterised in that：Described method is based on sea to put fruits into In the antistaling agent of polysaccharides, taken out after standing 5 ~ 9min, naturally dry.
- A kind of 2. method using the fresh-keeping fruit of algal polysaccharides according to claim 1, it is characterised in that：It is described to be based on sea The composition and its parts by weight of the antistaling agent of polysaccharides be：15 ~ 29 parts of algal polysaccharides, 3 ~ 6 parts of pomegranate rind extract, aloe extract 4 ~ 5 parts, 2.4 ~ 3.6 parts of tea polyphenol extract thing, 40 ~ 60 parts of sterilized water.
- A kind of 3. method using the fresh-keeping fruit of algal polysaccharides according to claim 2, it is characterised in that described marine alga Polysaccharide is extracted by the one or more in spirulina, seaweed, sargassum, dulse, green alga, loose algae.
- 4. a kind of method using the fresh-keeping fruit of algal polysaccharides according to claim 1, it is characterised in that described based on sea The antistaling agent preparation method of polysaccharides comprises the following steps：1）Marine alga raw material to be cleaned, marine alga is extracted using sonioation method, suction filtration takes filtrate, obtains the leaching liquor containing algal polysaccharides, It is standby；2）Gained filtrate in step 1 is taken, organic solvent is added, is stood after stirring, wait to be layered, removes upper strata part containing chlorophyll layer Liquid, it is standby；3）Filtrate in step 2 is taken, carries out de-salting operation, it is standby；4）Take liquid in step 3 concentrated, crystallized, filtered, dried, obtain algal polysaccharides sterling；5）By weight, the algal polysaccharides in step 4 are taken, add pomegranate rind extract, aloe extract, tea polyphenol extract The antistaling agent based on algal polysaccharides is made after stirring in thing, sterilized water.
- A kind of 5. method using the fresh-keeping fruit of algal polysaccharides according to claim 4, it is characterised in that described preparation Sonioation method uses Ultrasonic Cell Disruptor in step 1, and ultrasonic frequency is 21 ~ 24KHz, and extraction time is 15 ~ 23min.
- A kind of 6. method using the fresh-keeping fruit of algal polysaccharides according to claim 4, it is characterised in that described preparation The organic solvent added in step 2 is 0.2 ~ 0.5 times of volume of filtrate, and Elements in Organic Solvents is：Ethyl acetate and active peptides, The ratio of weight successively of composition is 1:0.2 ~ 0.4, the amino acid sequence of active peptides is： HSHACACSKHSHAGTLSCLCRVLHPGKLCACVNCSR。
- A kind of 7. method using the fresh-keeping fruit of algal polysaccharides according to claim 4, it is characterised in that described preparation De-salting operation uses ionodialysis method in step 3.
- A kind of 8. antistaling agent preparation method based on algal polysaccharides according to claim 4, it is characterised in that described system In standby step 4, concentrate and be concentrated under reduced pressure using Rotary Evaporators, liquid concentration to water content is 68 ~ 77%, and crystallization operation is addition 3 The concentration of ~ 6 times of volumes is 90 ~ 96% ethanol.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754525A (en) * | 2019-10-31 | 2020-02-07 | 华南农业大学 | Prevention and treatment method for cold damage of pitaya |
| CN112293482A (en) * | 2020-11-05 | 2021-02-02 | 西南大学 | Fruit preservative and method for delaying senescence of citrus fruits and prolonging fruit-bearing preservation period |
-
2017
- 2017-08-15 CN CN201710698332.5A patent/CN107494705A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754525A (en) * | 2019-10-31 | 2020-02-07 | 华南农业大学 | Prevention and treatment method for cold damage of pitaya |
| CN112293482A (en) * | 2020-11-05 | 2021-02-02 | 西南大学 | Fruit preservative and method for delaying senescence of citrus fruits and prolonging fruit-bearing preservation period |
| CN112293482B (en) * | 2020-11-05 | 2022-04-19 | 西南大学 | Fruit preservative and method for delaying senescence of citrus fruits and prolonging fruit-bearing preservation period |
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