CN109503678B - Method for extracting and purifying trehalose from cordyceps militaris, trehalose product and application thereof - Google Patents
Method for extracting and purifying trehalose from cordyceps militaris, trehalose product and application thereof Download PDFInfo
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- CN109503678B CN109503678B CN201811598953.7A CN201811598953A CN109503678B CN 109503678 B CN109503678 B CN 109503678B CN 201811598953 A CN201811598953 A CN 201811598953A CN 109503678 B CN109503678 B CN 109503678B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/04—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
Abstract
The invention relates to the field of medicines, and particularly relates to a method for extracting and purifying trehalose from cordyceps militaris, a trehalose product and application of the trehalose product. The method for extracting and purifying trehalose from cordyceps militaris comprises the steps of mixing a crushed cordyceps militaris raw material with an alcohol solution according to a mass ratio of 1: 10-50, carrying out ultrasonic extraction, and filtering to obtain a cordyceps militaris extracting solution; crystallizing the cordyceps militaris extracting solution to obtain trehalose crystals; the trehalose crystals were purified. The trehalose is extracted and purified from the cordyceps militaris, so that the trehalose in the cordyceps militaris can be fully utilized, the efficacy of the cordyceps militaris is utilized in a targeted manner, and the utilization rate of the cordyceps militaris is improved.
Description
Technical Field
The invention relates to the field of medicines, and particularly relates to a method for extracting and purifying trehalose from cordyceps militaris, a trehalose product and application of the trehalose product.
Background
Cordyceps militaris (Cordycepsmithia) also called Cordyceps militaris is of the same genus and different species with Chinese precious Chinese medicinal material Cordyceps (Cordyeepssinesis).
Cordyceps militaris is a kind of ascomycete, and sexual reproduction is carried out by using the heterothallic cooperation. The non-sexual type is paecilomyces chrysalis. The mature sporocarp can form ascospore (propagation unit), the spore spreads with wind, the spore falls on proper insect body, and starts to germinate to form mycelium. The mycelium continuously develops and begins to spread into the pupa body, so that the pupa body can be infected by fungi, the tissue in the pupa body is decomposed, the nutrition in the pupa body is used as a material and an energy source for the growth and development of the pupa body, and finally, the interior of the pupa body is completely decomposed.
The traditional Chinese medicine considers that the cordyceps militaris has the effects of strengthening body resistance and consolidating the constitution, has obvious curative effects on senile chronic bronchitis and pulmonary heart disease, can improve the detoxifying capability of the liver and has the effect of protecting the liver.
At present, the cordyceps militaris is usually directly used as food or used as medicated diet for human bodies by being combined with some traditional Chinese medicines.
In such a way, the pertinence is poor, and the utilization rate of the function and the value of a certain specific component in the cordyceps militaris is low.
Disclosure of Invention
In view of the above, an object of the embodiments of the present invention is to provide a method for extracting and purifying trehalose from cordyceps militaris, a trehalose product and an application thereof, so as to solve the above technical problems.
In a first aspect, an embodiment of the present invention provides a method for extracting and purifying trehalose from cordyceps militaris, including: mixing the crushed cordyceps militaris raw material with an alcohol solution according to the mass ratio of 1: 10-50, and performing ultrasonic extraction to obtain a cordyceps militaris extracting solution; crystallizing the cordyceps militaris extracting solution to obtain trehalose crystals; the trehalose crystals were purified.
The trehalose is extracted and purified from the cordyceps militaris, so that the trehalose in the cordyceps militaris can be fully utilized, and the efficacy of the cordyceps militaris is utilized in a targeted manner. Trehalose is widely applied, and can be used as an excellent activity protective agent for protein drugs, enzymes, vaccines and other biological products, also can be used as an important component of cosmetics for keeping cell activity and moisture, and can be used as a unique food ingredient for preventing food deterioration, keeping fresh flavor of food and improving food quality. Therefore, the trehalose is extracted and purified from the cordyceps militaris, so that the utilization rate of the cordyceps militaris can be effectively improved.
In some embodiments of the invention, the time of ultrasonic extraction is 30-120 minutes.
The ultrasonic extraction method can make the solvent rapidly enter the solid substance, and dissolve the organic components contained in the substance in the solvent as completely as possible to obtain the multi-component mixed extract. The trehalose is extracted from the cordyceps militaris by ultrasonic extraction, so that an alcohol solution can quickly enter the cordyceps militaris, and organic ingredients in the cordyceps militaris are dissolved in a solvent as completely as possible. The ultrasonic extraction strengthens the extraction and separation process, can effectively improve the extraction and separation rate, shorten the extraction time, save the cost and even improve the quality and the yield of products. The inventor finds that when the ultrasonic extraction time is 30-120 minutes, the extraction effect is good, and the economic effect is good.
In some embodiments of the invention, after the ultrasonic extraction is carried out for 30-120 minutes, the mixed extract obtained by the ultrasonic extraction is subjected to solid-liquid separation to prepare the cordyceps militaris extract.
The solid-liquid separation is carried out on the mixed extracting solution after the ultrasonic extraction, solid matters which are not dissolved are removed, and the solution formed by dissolving in the solvent, namely the cordyceps militaris extracting solution, is collected.
Further optionally, the mixed extract obtained by ultrasonic extraction is subjected to solid-liquid separation by adopting a filtering or suction filtration method to obtain cordyceps militaris extract.
Through filtering, impurities can be removed, the cordyceps militaris extracting solution with good quality is collected, and the efficiency of extracting trehalose from the cordyceps militaris extracting solution is improved. The extraction and filtration mode can further improve the efficiency of removing impurities in the cordyceps militaris extracting solution, and save time.
In some embodiments of the invention, the step of crystallizing the cordyceps militaris extracting solution is to stand the cordyceps militaris extracting solution for 8-72 hours to separate out trehalose crystals.
Trehalose crystals are separated out from the cordyceps militaris extracting solution by adopting a standing method, and the trehalose crystals can be effectively separated out from the cordyceps militaris extracting solution. Standing for 8-72 hours to sufficiently separate out more trehalose crystals from the cordyceps militaris extracting solution.
In some embodiments of the invention, the precipitated trehalose crystals are further subjected to impurity removal and/or drying.
The purity of the trehalose crystals can be further improved by removing impurities from the precipitated trehalose crystals. The trehalose crystals are dried, so that the moisture and other easily volatile matters in the trehalose crystals can be further removed, and the purity of the trehalose crystals is further guaranteed.
In some embodiments of the invention, the trehalose crystals are purified by adding water and absolute ethyl alcohol to the dried trehalose crystals in sequence for dissolving and then recrystallizing; wherein the volume of the absolute ethyl alcohol is 200 times of the volume of the water.
The trehalose crystals are purified by adopting a recrystallization method, so that other impurities in the trehalose crystals can be further dissolved in the solvent, and when the crystallization is carried out again, the impurities in the trehalose crystals can be removed, and the purity of the trehalose is further improved.
In some embodiments of the invention, the cordyceps militaris raw material is crushed to a particle size of the cordyceps militaris raw material below 40 mesh.
The cordyceps militaris raw material is sieved before extraction, so that impurities in the cordyceps militaris raw material can be removed, and subsequent purification is easier to perform. The trehalose crystals obtained have less impurities.
In some embodiments of the invention, the alcohol solution is selected from an ethanol solution having a volume fraction of 80% to 100%.
The ethanol solution can effectively dissolve the trehalose in the cordyceps militaris raw material, thereby ensuring that the trehalose is extracted from the cordyceps militaris raw material. The ethanol solution with the volume fraction of 80-100% can effectively dissolve cordyceps militaris raw materials and extract trehalose.
In a second aspect, the embodiment of the invention provides a trehalose product prepared by a method for extracting and purifying trehalose from cordyceps militaris.
By extracting and purifying trehalose from the cordyceps militaris, the utilization rate of the cordyceps militaris is effectively improved, the utilization rate of single components of the cordyceps militaris is improved, and the targeted utilization of the cordyceps militaris is improved.
In a third aspect, the embodiment of the invention provides an application of a trehalose product in biological medical activity protection, moisture-preserving cosmetics and food preservation.
The trehalose product extracted from the cordyceps militaris can be applied to biological medical activity protection, moisture-keeping cosmetics and food preservation. Thereby effectively improving the activity of the biological medicine, improving the moisturizing effect of the cosmetics and improving the freshness of food.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In the description of the present invention, it should be noted that the terms "first", "second", and the like are used only for distinguishing the description, and are not intended to indicate or imply relative importance.
The method for extracting and purifying trehalose from cordyceps militaris, trehalose products and application thereof of the embodiment of the invention are specifically described below.
The method for extracting and purifying trehalose from cordyceps militaris comprises the steps of mixing crushed cordyceps militaris raw materials and an alcohol solution according to a mass ratio of 1: 10-50, and carrying out ultrasonic extraction to obtain cordyceps militaris extracting solution.
The inventor finds that trehalose contained in cordyceps militaris can protect the activity of protein drugs, enzymes, vaccines and other biological products in research practice. Trehalose contained in cordyceps militaris can also play a good role in maintaining cell activity. Trehalose contained in cordyceps militaris also has a good effect on food preservation. Therefore, the trehalose product in the cordyceps militaris is extracted and separated, so that the utilization rate of the cordyceps militaris can be effectively improved, the utilization rate of a single component of the cordyceps militaris is improved, and the effect of the cordyceps militaris is utilized in a targeted manner.
Mixing the crushed cordyceps militaris raw material with an alcohol solution according to the mass ratio of 1: 10-50, and performing ultrasonic extraction to obtain a cordyceps militaris extracting solution. Not only can dissolve the cordyceps militaris raw material in the alcohol solution to a large extent, but also can effectively extract the trehalose. The ultrasonic extraction method can make the solvent rapidly enter the solid substance, and dissolve the organic components contained in the substance in the solvent as completely as possible to obtain the multi-component mixed extract.
Further, the cordyceps militaris raw material is crushed to the particle size of the cordyceps militaris raw material which is below 40 meshes.
Further optionally, the cordyceps militaris raw material is crushed to the particle size of the cordyceps militaris raw material being 100 mesh sieve and above 100 mesh sieve.
The cordyceps militaris raw material is sieved before extraction, so that impurities in the cordyceps militaris raw material can be removed, and subsequent purification is easier to perform. The trehalose crystals obtained have less impurities.
Further optionally, the cordyceps militaris raw material is crushed and then sieved. The sieving can be carried out by using sieving equipment commonly used in the field. When sieving, the material below the sieve body is taken.
Further, mixing the crushed cordyceps militaris raw material with an alcohol solution according to a mass ratio of 1: 10-50.
After the cordyceps militaris raw material and the alcohol solution are mixed according to the mass ratio of 1: 10-50, the cordyceps militaris raw material can be effectively dissolved, and trehalose in the cordyceps militaris raw material can be fully extracted.
Further optionally, the alcohol solution is selected from 80-100% by volume of ethanol solution.
The ethanol solution can effectively dissolve the trehalose in the cordyceps militaris raw material, thereby ensuring that the trehalose is extracted from the cordyceps militaris raw material. The ethanol solution with the volume fraction of 80-100% can effectively dissolve cordyceps militaris raw materials and extract trehalose.
In other alternative embodiments of the present invention, other alcoholic solutions that can be used for purification in the art may also be selected. Such as ethylene glycol, glycerol, and the like.
It should be noted that when the other types of alcohol are selected, a mixed solution of alcohol and water is selected to ensure complete dissolution of the cordyceps militaris raw material.
Further, extracting the cordyceps militaris extract by ultrasonic.
The trehalose is extracted from the cordyceps militaris by ultrasonic extraction, so that an alcohol solution can quickly enter the cordyceps militaris, and organic ingredients in the cordyceps militaris are dissolved in a solvent as completely as possible. The ultrasonic extraction strengthens the extraction and separation process, can effectively improve the extraction and separation rate, shorten the extraction time, save the cost and even improve the quality and the yield of products.
According to the embodiment of the invention, after the crushed cordyceps militaris raw material and an alcohol solution are mixed according to the mass ratio of 1: 10-50, cordyceps militaris extracting solution is obtained through ultrasonic extraction, and the extraction rate can reach more than 88%; the biological activity of the trehalose is maintained to the maximum extent.
Further optionally, the time for ultrasonic extraction is 30-120 minutes.
The inventor finds that when the ultrasonic extraction time is 30-120 minutes, the extraction effect is good, and the economic effect is good.
Further, after ultrasonic extraction is carried out for 30-120 minutes, solid-liquid separation is carried out on the mixed extracting solution of the ultrasonic extraction, and the cordyceps militaris extracting solution is prepared.
The solid-liquid separation is carried out on the mixed extracting solution after the ultrasonic extraction, solid matters which are not dissolved are removed, and the solution formed by dissolving in the solvent, namely the cordyceps militaris extracting solution, is collected.
Further optionally, the mixed extract obtained by ultrasonic extraction is subjected to solid-liquid separation by adopting a filtering or suction filtration method to obtain cordyceps militaris extract.
Through filtering, impurities can be removed, the cordyceps militaris extracting solution with good quality is collected, and the efficiency of extracting trehalose from the cordyceps militaris extracting solution is improved. The extraction and filtration mode can further improve the efficiency of removing impurities in the cordyceps militaris extracting solution, and save time.
Further, crystallizing the cordyceps militaris extracting solution to obtain trehalose crystals.
The trehalose crystal is obtained by a crystallization mode, so that the drug effect of trehalose in the original cordyceps militaris can be maintained to the maximum extent, and a trehalose product with good performance is obtained.
Further optionally, the step of crystallizing the cordyceps militaris extracting solution is to keep the cordyceps militaris extracting solution standing for 8-72 hours to separate out trehalose crystals.
Trehalose crystals are separated out from the cordyceps militaris extracting solution by adopting a standing method, and the trehalose crystals can be effectively separated out from the cordyceps militaris extracting solution. Standing for 8-72 hours to sufficiently separate out more trehalose crystals from the cordyceps militaris extracting solution.
According to the embodiment of the invention, trehalose crystals are separated out in a crystallization mode, and the purity can reach 85% or more through primary crystallization.
Further, the precipitated trehalose crystals are subjected to impurity removal and drying.
The purity of the trehalose crystals can be further improved by removing impurities from the precipitated trehalose crystals. The trehalose crystals are dried, so that the moisture and other easily volatile matters in the trehalose crystals can be further removed, and the purity of the trehalose crystals is further guaranteed.
In other alternative embodiments of the present invention, one of the impurity removing and drying operations may be selected.
Further optionally, the impurity removal is to filter the solution from which the trehalose crystals precipitate after standing to remove impurity liquid which does not crystallize yet, thereby improving the purity of the crystallized trehalose crystals.
Further alternatively, the drying may be performed in a drying setting or an air drying setting, or both.
Further, the trehalose crystals were purified.
The trehalose crystals are purified, so that the purity of the trehalose crystals can be effectively improved, and the excellent performance of subsequently obtained trehalose products is guaranteed.
Further optionally, the trehalose crystals are purified by adding water and absolute ethyl alcohol to the dried trehalose crystals in sequence for dissolving and then recrystallizing.
Specifically, firstly, water is added into the prepared trehalose crystals to completely dissolve the trehalose crystals in the water, and then absolute ethyl alcohol is added into the trehalose aqueous solution to extract and separate the trehalose, so that the trehalose crystals are recrystallized and separated from the solution, and the purification of the trehalose crystals is realized.
Further optionally, when water and absolute ethanol are sequentially added to the dried trehalose crystals for dissolution and then recrystallization is performed, the volume of the absolute ethanol is 200 times of the volume of the water.
Since trehalose is easily dissolved in water, when the trehalose is recrystallized and purified, only less water needs to be added to dissolve the trehalose crystals, and then more ethanol needs to be added as an extracting solution in order to ensure that enough trehalose crystals can be extracted.
The inventors have found, in long-term studies, that when the volume of absolute ethanol is 200 times the volume of water, trehalose in the trehalose solution can be sufficiently extracted and trehalose crystals can be precipitated.
In other alternative embodiments of the present invention, the above absolute ethanol may be replaced by other alcoholic solutions that can be used for purification in the art. Such as ethylene glycol, glycerol, and the like.
It should be noted that the alcohol solution should be anhydrous alcohol solution to ensure the extraction effect.
According to the embodiment of the invention, the trehalose crystals are purified by secondary crystallization, and the purity of the trehalose can reach more than 90%.
It is noted that in other alternative embodiments of the present invention, when further improvements in trehalose crystal purity are desired, the purification process described above, i.e., multiple crystallizations, may be repeated. For example, recrystallization may be selected two, three, or more times.
The method for extracting and purifying trehalose from cordyceps militaris provided by the embodiment of the invention comprises the steps of mixing crushed cordyceps militaris raw materials with an alcohol solution in a mass ratio of 1: 10-50, and carrying out ultrasonic extraction to obtain cordyceps militaris extracting solution; crystallizing the cordyceps militaris extracting solution to obtain trehalose crystals; the trehalose crystals were purified. The content of trehalose can reach more than 90% after separation and purification. The method has simple production process and mature technology, and is suitable for large-scale production.
Some embodiments of the invention also provide a trehalose product prepared by the method for extracting and purifying trehalose from cordyceps militaris. The trehalose product has high trehalose content and good action effect.
Specifically, the cordyceps militaris trehalose product has few impurities, high activity and wide application. Can be used as raw material of food, cosmetic, and health product.
Some embodiments of the invention also provide application of the trehalose product in biological medical activity protection, moisture-preserving cosmetics and food preservation.
Specifically, the trehalose product can be used as an excellent activity protective agent for protein drugs, enzymes, vaccines and other biological products, is an important component of cosmetics for keeping cell activity and moisture, can be used as a unique food ingredient for preventing food deterioration, keeping fresh flavor of food and improving food quality, and greatly expands the function of the trehalose as natural edible sweet sugar. Therefore, the method can be widely applied to the fields of biological medicine, food, cosmetics, health care products and the like, and has wide market development prospect.
The features and properties of the present invention are further described in detail below with reference to examples:
example 1
The method for extracting and purifying trehalose from cordyceps militaris provided by the embodiment comprises the following steps:
pulverizing Cordyceps militaris, sieving with 40 mesh sieve, mixing with 80% ethanol extractive solution at a material liquid mass ratio of 1:10, ultrasonic extracting for 30 min, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; pumping the extracting solution into a glass ware, standing for 8 hours, and crystallizing; filtering and drying to obtain trehalose crystals. Taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing, wherein the volume of the absolute ethyl alcohol is 100 times of that of the water.
The prepared trehalose product is detected, and the detection extraction rate reaches 88.92%. The purity of the trehalose reaches 92.68%.
Example 2
The method for extracting and purifying trehalose from cordyceps militaris provided by the embodiment comprises the following steps:
pulverizing Cordyceps militaris, sieving with 100 mesh sieve, mixing with the extractive solution anhydrous ethanol at a feed liquid mass ratio of 1: 50, extracting with ultrasonic wave for 2 hr, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; separating trehalose from cordyceps militaris: pumping the extracting solution into a glass ware, standing for 72 hours, and crystallizing; filtering and drying to obtain trehalose crystals. Taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing. Wherein the volume of the absolute ethyl alcohol is 200 times of the volume of the water.
The extraction rate of the prepared trehalose product is up to 90.45 percent by detecting. The purity of the trehalose reaches 96.34 percent.
Example 3
The method for extracting and purifying trehalose from cordyceps militaris provided by the embodiment comprises the following steps:
pulverizing Cordyceps militaris, sieving with 60 mesh sieve, mixing with the extractive solution anhydrous ethanol at a feed liquid mass ratio of 1: 20, extracting with ultrasonic wave for 1 hr, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; separating trehalose from cordyceps militaris: pumping the extracting solution into a glass vessel, standing for 24 hours, and crystallizing; filtering and drying to obtain trehalose crystals. Taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing. Wherein the volume of the absolute ethyl alcohol is 150 times of the volume of the water.
The extraction rate of the prepared trehalose product is 91.24 percent by detecting. The purity of the trehalose reaches 95.58%.
Example 4
The method for extracting and purifying trehalose from cordyceps militaris provided by the embodiment comprises the following steps:
pulverizing Cordyceps militaris, sieving with 80 mesh sieve, mixing with the extractive solution anhydrous ethanol at a feed liquid mass ratio of 1: 30, extracting with ultrasonic wave for 1 hr, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; separating trehalose from cordyceps militaris: pumping the extracting solution into a glass vessel, standing for 48 hours, and crystallizing; filtering and drying to obtain trehalose crystals. Taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing. Wherein the volume of the absolute ethyl alcohol is 160 times of the volume of the water.
The extraction rate of the prepared trehalose product is 90.84 percent by detecting. The purity of the trehalose reaches 95.89%.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. A method for extracting and purifying trehalose from cordyceps militaris is characterized by comprising the following steps:
pulverizing Cordyceps militaris, sieving with 40 mesh sieve, mixing with 80% ethanol extractive solution at a material liquid mass ratio of 1:10, ultrasonic extracting for 30 min, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; pumping the extracting solution into a glass ware, standing for 8 hours, and crystallizing; filtering and drying to obtain trehalose crystals; taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing, wherein the volume of the absolute ethyl alcohol is 100 times of that of the water; or
Pulverizing Cordyceps militaris, sieving with 100 mesh sieve, mixing with the extractive solution anhydrous ethanol at a feed liquid mass ratio of 1: 50, extracting with ultrasonic wave for 2 hr, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; separating trehalose from cordyceps militaris: pumping the extracting solution into a glass ware, standing for 72 hours, and crystallizing; filtering and drying to obtain trehalose crystals; taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing; wherein the volume of the absolute ethyl alcohol is 200 times of the volume of the water; or
Pulverizing Cordyceps militaris, sieving with 60 mesh sieve, mixing with the extractive solution anhydrous ethanol at a feed liquid mass ratio of 1: 20, extracting with ultrasonic wave for 1 hr, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; separating trehalose from cordyceps militaris: pumping the extracting solution into a glass vessel, standing for 24 hours, and crystallizing; filtering and drying to obtain trehalose crystals; taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing; wherein the volume of the absolute ethyl alcohol is 150 times of the volume of the water; or
Pulverizing Cordyceps militaris, sieving with 80 mesh sieve, mixing with the extractive solution anhydrous ethanol at a feed liquid mass ratio of 1: 30, extracting with ultrasonic wave for 1 hr, and vacuum filtering to obtain brown yellow Cordyceps militaris extractive solution; separating trehalose from cordyceps militaris: pumping the extracting solution into a glass vessel, standing for 48 hours, and crystallizing; filtering and drying to obtain trehalose crystals; taking trehalose crystals, sequentially adding water and absolute ethyl alcohol, dissolving with water, adding ethanol, and recrystallizing; wherein the volume of the absolute ethyl alcohol is 160 times of the volume of the water.
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Non-Patent Citations (4)
| Title |
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| 刘艳芳，等.蛹虫草子实体中海藻糖含量的测定.《菌物学报》.2011,第30卷(第3期),472-476. * |
| 海藻糖的特性、功能及应用;靳文斌，等;《精细与专用化学品》;20150131;第23卷(第1期);30-33 * |
| 蛹虫草子实体中海藻糖含量的测定;刘艳芳，等;《菌物学报》;20110515;第30卷(第3期);472-476 * |
| 靳文斌，等.海藻糖的特性、功能及应用.《精细与专用化学品》.2015,第23卷(第1期),30-33. * |
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