CN107488621A - A kind of denitrification denitrogenation composite bacteria agent and its preparation method and application - Google Patents
A kind of denitrification denitrogenation composite bacteria agent and its preparation method and application Download PDFInfo
- Publication number
- CN107488621A CN107488621A CN201710947770.0A CN201710947770A CN107488621A CN 107488621 A CN107488621 A CN 107488621A CN 201710947770 A CN201710947770 A CN 201710947770A CN 107488621 A CN107488621 A CN 107488621A
- Authority
- CN
- China
- Prior art keywords
- composite bacteria
- denitrification denitrogenation
- achromobacter
- bacteria agent
- anthropi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
Abstract
The invention discloses a kind of denitrification denitrogenation composite bacteria agent, is as made from the strain of volumes below percentage：Secondary coccus 20 30%, anthropi 20 30%, achromobacter 40 60%.The invention also discloses the preparation method of the denitrification denitrogenation composite bacteria agent and its application in sewage disposal containing nitrate nitrogen.The synergy that the denitrification denitrogenation composite bacteria agent of the present invention passes through secondary coccus, anthropi and achromobacter, the high excellent properties of the clearance of adaptable strong, processing time is short, nitrate nitrogen degradation rate and COD, applied in sewage, significantly improve the degradation rate of nitrate from waste water nitrogen and COD clearance, cost is greatlyd save, is had broad application prospects.
Description
Technical field
The invention belongs to environment bioengineering and water treatment field, and in particular to a kind of denitrification denitrogenation composite bacteria agent and its
Preparation method and application.
Background technology
With the fast development of China's economic, the pollutant such as substantial amounts of nitrogen, phosphorus is discharged into water body, causes the serious rich battalion of water body
Fosterization, constituted a threat to environment for human survival the reason for one of.The pollution of domestic Water is with nitrate nitrogen and nitrite nitrogen
The pollution of form nitrogen is the most serious, and biological removal of nitrogen is to remove one of Water pollution most efficient method.
Bio-denitrification technology is to realize that nitrate nitrogen and nitrite nitrogen etc. pollute in waste water using the denitrification of microorganism
The removal of thing, it presently, there are the problems such as denitrification rate is relatively low, treatment effeciency is poor.To solve this problem, typically with drop
The methods of reduction process load, increase sewage disposal time, lifting sludge settling, improves denitrification treatment effect, but these
Measure treatment effect is very unstable, can also increase sewage disposal expense, and increase economic pressures.
The content of the invention
The invention provides the anti-nitre of a kind of strong adaptability, processing time is short, nitrate nitrogen degradation rate and COD clearance
Change denitrification compound bacteria agent.
Present invention also offers the preparation method and application of the denitrification denitrogenation composite bacteria agent.
The purpose of the present invention is achieved by the following technical solution：
A kind of denitrification denitrogenation composite bacteria agent, it is as made from the strain of volumes below percentage：Secondary coccus 20-30%, pale bar
Bacterium 20-30%, achromobacter 40-60%.
Described, the viable count of secondary coccus is 1 × 107-1×109CFU/g；The viable count of the anthropi be 1 ×
107-1×1010CFU/g；The viable count of the achromobacter is 1 × 105-1×109CFU/g。
A kind of preparation method of denitrification denitrogenation composite bacteria agent, is prepared by following steps：
1）Each two ring of secondary coccus, anthropi and achromobacter of inclined-plane conservation is taken respectively, is inoculated in respectively in solid medium
Culture, 25-35 DEG C of cultivation temperature, incubation time 1-3d, respectively secondary coccus, anthropi and achromobacter activated spawn；
2）Enriched medium is dispensed into 250mL triangular flasks, 50-100mL/ bottles；By secondary coccus, anthropi and achromobacter
Activated spawn shaking table shaken cultivation, shaking table shaken cultivation rotating speed are carried out in enriched medium respectively at accessing two rings on inclined-plane
120-180r/min, 25-35 DEG C of shaking table shaken cultivation temperature, shaking table shaken cultivation time 1-3d, respectively secondary coccus, pale bar
Bacterium and the seed liquor of achromobacter；
3）The seed liquor of secondary coccus, anthropi and achromobacter is inoculated in Liquid Culture respectively according to 2-10% inoculum concentration
Base carries out shaking table shaken cultivation, shaking table shaken cultivation rotating speed 120-180r/min, 25-35 DEG C of shaking table shaken cultivation temperature, works as pair
Viable count reaches 1 × 10 in coccus nutrient solution7-1×109Viable count reaches 1 × 10 in CFU/g, anthropi nutrient solution7-1×
1010Viable count reaches 1 × 10 in CFU/g and achromobacter nutrient solution5-1×109CFU/g, stop culture respectively；
4）By step 3）Obtained bacterium solution is according to percent by volume：Secondary coccus 20-30%, pale dry bacterium 30-40%, and achromobacter
40-60%, mixing, obtains composite bacteria liquid；
5）50-100mL composite bacteria liquids are taken, 6000r/min centrifugations remove supernatant, obtain wet thallus；Wet thallus is resuspended in 50-
In 100mL physiological saline, centrifugation, repeat 2-3 times, obtain compound bacteria suspension；
The concentration of the sterile saline is 0.9%；
6）Compound bacteria suspension and protective agent are well mixed, is spray-dried, 120-180 DEG C of spray drying EAT, enters stream
12-17ml/min is measured, obtains denitrification denitrogenation composite bacteria agent.
Described, solid medium is beef-protein medium, concrete composition：Beef extract 5g/L, peptone 10g/
L, NaCl 5g/L, agar 15-20g/L, remaining is water.
Described, the composition of enriched medium：KNO32-5g/L, peptone 5-10g/L, beef extract 5-10g/L, remaining
For water.
Described, the composition of fluid nutrient medium：Sodium acetate 1-20g/L, KNO30.1-5g/L, K2HPO4·3H2O 0.1-
2g/L, MgSO4·7H2O 0.1-1g/L, remaining is water.
Described, compound bacteria suspension and protectant mass ratio are 100：5-15；The protective agent is beta-schardinger dextrin, malt
One kind in dextrin and carboxymethyl cellulose.
A kind of application of denitrification denitrogenation composite bacteria agent, the denitrification denitrogenation composite bacteria agent is at sewage containing nitrate nitrogen
Application in reason.
Described, inoculum concentration of the denitrification denitrogenation composite bacteria agent in every liter of sewage is 1-15g, 25-35 DEG C for the treatment of temperature；
The C/N ratios of sewage are 4-10, pH 6-10.
Described, the maximum concentration of nitrate from waste water nitrogen is 700mg/L.
Described secondary coccus（Paracoccus sp.）Purchased from China General Microbiological culture presevation administrative center（Address：
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica）, numbering：CGMCC No.13607.
Described anthropi（ochrobactrum sp.）Purchased from China General Microbiological culture presevation administrative center（Ground
Location：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica）, numbering：CGMCC No.8839.
Described achromobacter（Achromobacter sp.）Purchased from China General Microbiological culture presevation administrative center
（Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica）, numbering：CGMCC No.7139.
Beneficial effects of the present invention：
1. the denitrification denitrogenation composite bacteria agent of the present invention has by the synergy of secondary coccus, anthropi and achromobacter
The high excellent properties of strong adaptability, processing time is short, nitrate nitrogen degradation rate and COD clearance, applied in sewage, significantly
The degradation rate of nitrate from waste water nitrogen and COD clearance are improved, cost is greatlyd save, has broad application prospects.
2. the preparation method of the present invention is simple, denitrification denitrogenation composite bacteria agent is prepared into bacterium powder, reduces volume, is easy to store
Deposit and transport, denitrification denitrogenation composite bacteria agent be thrown in sewage can prompt activation, it is adaptable, quickly form dominant bacteria
Group, Water cellulose content is significantly reduced, and accumulated without nitrite nitrogen, for utilizing the denitrification denitrogenation compound bacteria on a large scale
Agent processing sewage is significant.
Brief description of the drawings
Fig. 1 is nitrate nitrogen degradation rate curve.
Fig. 2 is COD clearance curves.
Embodiment
To further appreciate that the present invention, with reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of denitrification denitrogenation composite bacteria agent, it is as made from the strain of volumes below percentage：Secondary coccus 20%, anthropi
20%th, achromobacter 60%.
Described, the viable count of secondary coccus is 1 × 107-1×109CFU/g；The viable count of the anthropi be 1 ×
107-1×1010CFU/g；The viable count of the achromobacter is 1 × 105-1×109CFU/g。
A kind of preparation method of denitrification denitrogenation composite bacteria agent, is prepared by following steps：
1）Each two ring of secondary coccus, anthropi and achromobacter of inclined-plane conservation is taken respectively, is inoculated in respectively in solid medium
Culture, 25 DEG C, incubation time 3d of cultivation temperature, respectively secondary coccus, anthropi and achromobacter activated spawn；
2）Enriched medium is dispensed into 250mL triangular flasks, 50mL/ bottles；By the work of secondary coccus, anthropi and achromobacter
Change strain and shaking table shaken cultivation, shaking table shaken cultivation rotating speed are carried out in enriched medium respectively at accessing two rings on inclined-plane
180r/min, 25 DEG C of shaking table shaken cultivation temperature, shaking table shaken cultivation time 1d, respectively secondary coccus, anthropi and colourless
The seed liquor of bacillus；
3）The seed liquor of secondary coccus, anthropi and achromobacter is inoculated in into fluid nutrient medium respectively according to 2% inoculum concentration to enter
Row shaking table shaken cultivation, shaking table shaken cultivation rotating speed 180r/min, 35 DEG C of shaking table shaken cultivation temperature, when secondary coccus nutrient solution
Middle viable count reaches 1 × 107-1×109Viable count reaches 1 × 10 in CFU/g, anthropi nutrient solution7-1×1010CFU/g and
Viable count reaches 1 × 10 in achromobacter nutrient solution5-1×109CFU/g, stop culture respectively；
4）By step 3）Obtained bacterium solution is according to percent by volume：Secondary coccus 20%, anthropi 20%, achromobacter 60%, mix
Close, obtain composite bacteria liquid；
5）50mL composite bacteria liquids are taken, 6000r/min centrifugations remove supernatant, obtain wet thallus；Wet thallus is resuspended in 100mL physiology
In salt solution, centrifugation, repeat 2 times, obtain compound bacteria suspension；
The concentration of the sterile saline is 0.9%；
6）Compound bacteria suspension and protective agent are well mixed, are spray-dried, 180 DEG C of spray drying EAT, feed rate
12ml/min, obtain denitrification denitrogenation composite bacteria agent.
Described, solid medium is beef-protein medium, concrete composition：Beef extract 5g/L, peptone 10g/
L, NaCl 5g/L, agar 20g/L, remaining is water.
Described, the composition of enriched medium：KNO35g/L, peptone 5g/L, beef extract 10g/L, remaining is water.
Described, the composition of fluid nutrient medium：Sodium acetate 20g/L, KNO30.1g/L, K2HPO4·3H2O 0.1g/L,
MgSO4·7H2O1g/L, remaining is water.
Described, compound bacteria suspension and protectant mass ratio are 100：5；The protective agent is beta-schardinger dextrin, malt paste
One kind in essence and carboxymethyl cellulose.
A kind of application of denitrification denitrogenation composite bacteria agent, the denitrification denitrogenation composite bacteria agent is at sewage containing nitrate nitrogen
Application in reason.
Described, inoculum concentration of the denitrification denitrogenation composite bacteria agent in every liter of sewage is 15g, 25 DEG C for the treatment of temperature；Sewage
C/N ratios be 10, pH 6.
Described, the maximum concentration of nitrate from waste water nitrogen is 700mg/L.
Embodiment 2
A kind of denitrification denitrogenation composite bacteria agent, it is as made from the strain of volumes below percentage：Secondary coccus 25%, anthropi
25%th, achromobacter 50%.
Described, the viable count of secondary coccus is 1 × 107-1×109CFU/g；The viable count of the anthropi be 1 ×
107-1×1010CFU/g；The viable count of the achromobacter is 1 × 105-1×109CFU/g。
A kind of preparation method of denitrification denitrogenation composite bacteria agent, is prepared by following steps：
1）Each two ring of secondary coccus, anthropi and achromobacter of inclined-plane conservation is taken respectively, is inoculated in respectively in solid medium
Culture, 30 DEG C, incubation time 2d of cultivation temperature, respectively secondary coccus, anthropi and achromobacter activated spawn；
2）Enriched medium is dispensed into 250mL triangular flasks, 70mL/ bottles；By the work of secondary coccus, anthropi and achromobacter
Change strain and shaking table shaken cultivation, shaking table shaken cultivation rotating speed are carried out in enriched medium respectively at accessing two rings on inclined-plane
150r/min, 30 DEG C of shaking table shaken cultivation temperature, shaking table shaken cultivation time 2d, respectively secondary coccus, anthropi and colourless
The seed liquor of bacillus；
3）The seed liquor of secondary coccus, anthropi and achromobacter is inoculated in into fluid nutrient medium respectively according to 6% inoculum concentration to enter
Row shaking table shaken cultivation, shaking table shaken cultivation rotating speed 150r/min, 30 DEG C of shaking table shaken cultivation temperature, when secondary coccus nutrient solution
Middle viable count reaches 1 × 107-1×109Viable count reaches 1 × 10 in CFU/g, anthropi nutrient solution7-1×1010CFU/g and
Viable count reaches 1 × 10 in achromobacter nutrient solution5-1×109CFU/g, stop culture respectively；
4）By step 3）Obtained bacterium solution is according to percent by volume：Secondary coccus 25%, anthropi 25%, achromobacter 50%, mix
Close, obtain composite bacteria liquid；
5）80mL composite bacteria liquids are taken, 6000r/min centrifugations remove supernatant, obtain wet thallus；Wet thallus is resuspended in 80mL physiology
In salt solution, centrifugation, repeat 3 times, obtain compound bacteria suspension；
The concentration of the sterile saline is 0.9%；
6）Compound bacteria suspension and protective agent are well mixed, are spray-dried, 150 DEG C of spray drying EAT, feed rate
15ml/min, obtain denitrification denitrogenation composite bacteria agent.
Described, solid medium is beef-protein medium, concrete composition：Beef extract 5g/L, peptone 10g/
L, NaCl 5g/L, agar 18g/L, remaining is water.
Described, the composition of enriched medium：KNO33g/L, peptone 8g/L, beef extract 7g/L, remaining is water.
Described, the composition of fluid nutrient medium：Sodium acetate 10g/L, KNO33g/L, K2HPO4·3H2O 1g/L, MgSO4·
7H2O 0.5g/L, remaining is water.
Described, compound bacteria suspension and protectant mass ratio are 100：10；The protective agent is beta-schardinger dextrin, malt paste
One kind in essence and carboxymethyl cellulose.
A kind of application of denitrification denitrogenation composite bacteria agent, the denitrification denitrogenation composite bacteria agent is at sewage containing nitrate nitrogen
Application in reason.
Described, inoculum concentration of the denitrification denitrogenation composite bacteria agent in every liter of sewage is 8g, 30 DEG C for the treatment of temperature；Sewage
C/N ratios are 7, pH 8.
Described, the maximum concentration of nitrate from waste water nitrogen is 700mg/L.
Embodiment 3
A kind of denitrification denitrogenation composite bacteria agent, it is as made from the strain of volumes below percentage：Secondary coccus 30%, anthropi
30%th, achromobacter 40%.
Described, the viable count of secondary coccus is 1 × 107-1×109CFU/g；The viable count of the anthropi be 1 ×
107-1×1010CFU/g；The viable count of the achromobacter is 1 × 105-1×109CFU/g。
A kind of preparation method of denitrification denitrogenation composite bacteria agent, is prepared by following steps：
1）Each two ring of secondary coccus, anthropi and achromobacter of inclined-plane conservation is taken respectively, is inoculated in respectively in solid medium
Culture, 35 DEG C, incubation time 1d of cultivation temperature, respectively secondary coccus, anthropi and achromobacter activated spawn；
2）Enriched medium is dispensed into 250mL triangular flasks, 100mL/ bottles；By secondary coccus, anthropi and achromobacter
Activated spawn carries out shaking table shaken cultivation, shaking table shaken cultivation rotating speed respectively at accessing two rings on inclined-plane in enriched medium
120r/min, 35 DEG C of shaking table shaken cultivation temperature, shaking table shaken cultivation time 3d, respectively secondary coccus, anthropi and colourless
The seed liquor of bacillus；
3）The seed liquor of secondary coccus, anthropi and achromobacter is inoculated in fluid nutrient medium respectively according to 10% inoculum concentration
Carry out shaking table shaken cultivation, shaking table shaken cultivation rotating speed 120r/min, 25 DEG C of shaking table shaken cultivation temperature, when secondary coccus is cultivated
Viable count reaches 1 × 10 in liquid7-1×109Viable count reaches 1 × 10 in CFU/g, anthropi nutrient solution7-1×1010CFU/g
Reach 1 × 10 with viable count in achromobacter nutrient solution5-1×109CFU/g, stop culture respectively；
4）By step 3）Obtained bacterium solution is according to percent by volume：Secondary coccus 30%, anthropi 30%, achromobacter 40%, mix
Close, obtain composite bacteria liquid；
5）100mL composite bacteria liquids are taken, 6000r/min centrifugations remove supernatant, obtain wet thallus；Wet thallus is resuspended in 50mL physiology
In salt solution, centrifugation, repeat 3 times, obtain compound bacteria suspension；
The concentration of the sterile saline is 0.9%；
6）Compound bacteria suspension and protective agent are well mixed, are spray-dried, 120 DEG C of spray drying EAT, feed rate
17ml/min, obtain denitrification denitrogenation composite bacteria agent.
Described, solid medium is beef-protein medium, concrete composition：Beef extract 5g/L, peptone 10g/
L, NaCl 5g/L, agar 15g/L, remaining is water.
Described, the composition of enriched medium：KNO32g/L, peptone 10g/L, beef extract 5g/L, remaining is water.
Described, the composition of fluid nutrient medium：Sodium acetate 1g/L, KNO35g/L, K2HPO4·3H2O 2g/L, MgSO4·
7H2O 0.1g/L, remaining is water.
Described, compound bacteria suspension and protectant mass ratio are 100： 15；The protective agent is beta-schardinger dextrin, malt
One kind in dextrin and carboxymethyl cellulose.
A kind of application of denitrification denitrogenation composite bacteria agent, the denitrification denitrogenation composite bacteria agent is at sewage containing nitrate nitrogen
Application in reason.
Described, inoculum concentration of the denitrification denitrogenation composite bacteria agent in every liter of sewage is 1g, 35 DEG C for the treatment of temperature；Sewage
C/N ratios are 4, pH 10.
Described, the maximum concentration of nitrate from waste water nitrogen is 700mg/L.
Application in manual simulation's sewage
Table 1 simulates artificial sewage's composition
| Composition | Content（g/L） | Composition | Content（g/L） |
| KNO3 | 0.1-10 | MgSO4·7H2O | 0.01-1 |
| Sodium acetate | 0.1-10 | NaCl | 0.01-1 |
| Glucose | 0.1-5 | FeSO4·7H2O | 0.001-1 |
| K2HPO4 | 0.1-2 | MnSO4·4H2O | 0.001-1 |
Simulation artificial sewage is prepared according to table 1, nitrate nitrogen initial concentration is 700 mg/L, COD in regulation simulation artificial sewage
For 500mg/L.Experimental group 1-3 respectively according to embodiment 1-3 condition by prepared denitrification denitrogenation composite bacteria agent throw to
In 100mL simulation artificial sewages, control group 1-3 is according to the condition of embodiment 2, but control group 1 adds 8g pairs coccuses, control group
2 addition 8g anthropis, control group 3 add 8g achromobacters, blank control group only add simulation artificial sewage do not add it is any
Microbial inoculum, supernatant detection nitrate nitrogen and COD concentration are taken respectively, calculates nitrate nitrogen degradation rate and COD clearances.The water
The assay method of nitrate nitrogen content is disulfonic acid phenol colorimetric method in body, and COD assay method is potassium bichromate detection method.As a result
As depicted in figs. 1 and 2.
From Fig. 1 and Fig. 2, for experimental group 1 after 28h is cultivated, nitrate nitrogen degradation rate is that 88.70%, COD clearances are
81.99%；For experimental group 2 after 28h is cultivated, nitrate nitrogen degradation rate is that 99.16%, COD clearances are 93.75%；Experimental group 3 exists
After cultivating 28h, nitrate nitrogen degradation rate is that 92.34%, COD clearances are 86.21%；Control group 1 is after 28h is cultivated, nitrate
Nitrogen degradation rate is that 71.88%, COD clearances are 59.37%；For control group 2 after 28h is cultivated, nitrate nitrogen degradation rate is 14.38%,
COD clearances are 8.68%；For control group 3 after 28h is cultivated, nitrate nitrogen degradation rate is that 49.71%, COD clearances are 42.59%.
Claims (10)
1. a kind of denitrification denitrogenation composite bacteria agent, it is characterised in that be as made from the strain of volumes below percentage：Secondary coccus
20-30%, anthropi 20-30%, achromobacter 40-60%.
2. denitrification denitrogenation composite bacteria agent according to claim 1, it is characterised in that the viable count of the secondary coccus is 1
×107-1×109CFU/g；The viable count of the anthropi is 1 × 107-1×1010CFU/g；The viable bacteria of the achromobacter
Number is 1 × 105-1×109CFU/g。
3. the preparation method of the denitrification denitrogenation composite bacteria agent described in a kind of claim 1 or 2, it is characterised in that be by following
What step was prepared：
1）Each two ring of secondary coccus, anthropi and achromobacter of inclined-plane conservation is taken respectively, is inoculated in respectively in solid medium
Culture, 25-35 DEG C of cultivation temperature, incubation time 1-3d, respectively secondary coccus, anthropi and achromobacter activated spawn；
2）Enriched medium is dispensed into 250mL triangular flasks, 50-100mL/ bottles；By secondary coccus, anthropi and achromobacter
Activated spawn shaking table shaken cultivation, shaking table shaken cultivation rotating speed are carried out in enriched medium respectively at accessing two rings on inclined-plane
120-180r/min, 25-35 DEG C of shaking table shaken cultivation temperature, shaking table shaken cultivation time 1-3d, respectively secondary coccus, pale bar
Bacterium and the seed liquor of achromobacter；
3）The seed liquor of secondary coccus, anthropi and achromobacter is inoculated in Liquid Culture respectively according to 2-10% inoculum concentration
Base carries out shaking table shaken cultivation, shaking table shaken cultivation rotating speed 120-180r/min, 25-35 DEG C of shaking table shaken cultivation temperature, works as pair
Viable count reaches 1 × 10 in coccus nutrient solution7-1×109Viable count reaches 1 × 10 in CFU/g, anthropi nutrient solution7-1×
1010Viable count reaches 1 × 10 in CFU/g and achromobacter nutrient solution5-1×109CFU/g, stop culture respectively；
4）By step 3）Obtained bacterium solution is according to percent by volume：Secondary coccus 20-30%, pale dry bacterium 30-40%, and achromobacter
40-60%, mixing, obtains composite bacteria liquid；
5）50-100mL composite bacteria liquids are taken, 6000r/min centrifugations remove supernatant, obtain wet thallus；Wet thallus is resuspended in 50-
In 100mL physiological saline, centrifugation, repeat 2-3 times, obtain compound bacteria suspension；
The concentration of the sterile saline is 0.9%；
6）Compound bacteria suspension and protective agent are well mixed, is spray-dried, 120-180 DEG C of spray drying EAT, enters stream
12-17ml/min is measured, obtains denitrification denitrogenation composite bacteria agent.
4. the preparation method of denitrification denitrogenation composite bacteria agent according to claim 3, it is characterised in that the solid culture
Base is beef-protein medium, concrete composition：Beef extract 5g/L, peptone 10g/L, NaCl 5g/L, agar 15-20g/
L, remaining is water.
5. the preparation method of denitrification denitrogenation composite bacteria agent according to claim 3, it is characterised in that the enrichment culture
The composition of base：KNO32-5g/L, peptone 5-10g/L, beef extract 5-10g/L, remaining is water.
6. the preparation method of denitrification denitrogenation composite bacteria agent according to claim 3, it is characterised in that the Liquid Culture
The composition of base：Sodium acetate 1-20g/L, KNO30.1-5g/L, K2HPO4·3H2O 0.1-2g/L, MgSO4·7H2O 0.1-1g/
L, remaining is water.
7. the preparation method of denitrification denitrogenation composite bacteria agent according to claim 3, it is characterised in that the compound bacteria is hanged
Liquid and protectant mass ratio are 100：5-15；The protective agent is in beta-schardinger dextrin, maltodextrin and carboxymethyl cellulose
It is a kind of.
8. the application of the denitrification denitrogenation composite bacteria agent described in a kind of claim 1 or 2, it is characterised in that the denitrification takes off
Application of the nitrogen composite bacteria agent in sewage disposal containing nitrate nitrogen.
9. the application of denitrification denitrogenation composite bacteria agent according to claim 8, it is characterised in that the denitrification denitrogenation is answered
It is 1-15g to close inoculum concentration of the microbial inoculum in every liter of sewage, 25-35 DEG C for the treatment of temperature；The C/N ratios of sewage are 4-10, pH 6-10.
10. the application of denitrification denitrogenation composite bacteria agent according to claim 8, it is characterised in that nitrate from waste water nitrogen
Maximum concentration be 700mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710947770.0A CN107488621B (en) | 2017-10-12 | 2017-10-12 | Denitrification denitrification composite bacterial agent and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710947770.0A CN107488621B (en) | 2017-10-12 | 2017-10-12 | Denitrification denitrification composite bacterial agent and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107488621A true CN107488621A (en) | 2017-12-19 |
| CN107488621B CN107488621B (en) | 2021-06-01 |
Family
ID=60653699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710947770.0A Active CN107488621B (en) | 2017-10-12 | 2017-10-12 | Denitrification denitrification composite bacterial agent and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107488621B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110819557A (en) * | 2019-10-10 | 2020-02-21 | 中国农业大学 | Cellulose degradation composite microbial inoculum and preparation method and application thereof |
| CN112680358A (en) * | 2021-01-13 | 2021-04-20 | 华沃德源环境技术(济南)有限公司 | Process for continuously producing denitrifying bacteria agent |
| CN113716687A (en) * | 2021-09-08 | 2021-11-30 | 哈尔滨工业大学（深圳） | Biological denitrification strengthening method based on exogenous beta-cyclodextrin addition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122332A (en) * | 2013-02-27 | 2013-05-29 | 中蓝连海设计研究院 | Achromobacter xylosoxidans subsp.xylosoxidans LH-N25 and heterotrophic nitrification and aerobic denitrification microorganism bactericide and preparation method and use thereof |
| CN105779344A (en) * | 2016-03-29 | 2016-07-20 | 江苏蓝星化工环保股份有限公司 | Composite microbial agent |
-
2017
- 2017-10-12 CN CN201710947770.0A patent/CN107488621B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122332A (en) * | 2013-02-27 | 2013-05-29 | 中蓝连海设计研究院 | Achromobacter xylosoxidans subsp.xylosoxidans LH-N25 and heterotrophic nitrification and aerobic denitrification microorganism bactericide and preparation method and use thereof |
| CN105779344A (en) * | 2016-03-29 | 2016-07-20 | 江苏蓝星化工环保股份有限公司 | Composite microbial agent |
Non-Patent Citations (1)
| Title |
|---|
| 黄郑郑等: "异养硝化-好氧反硝化菌协同竞争对脱氮特性的影响", 《环境与科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110819557A (en) * | 2019-10-10 | 2020-02-21 | 中国农业大学 | Cellulose degradation composite microbial inoculum and preparation method and application thereof |
| CN110819557B (en) * | 2019-10-10 | 2021-07-09 | 中国农业大学 | Cellulose degradation composite microbial inoculum and preparation method and application thereof |
| CN112680358A (en) * | 2021-01-13 | 2021-04-20 | 华沃德源环境技术(济南)有限公司 | Process for continuously producing denitrifying bacteria agent |
| CN113716687A (en) * | 2021-09-08 | 2021-11-30 | 哈尔滨工业大学（深圳） | Biological denitrification strengthening method based on exogenous beta-cyclodextrin addition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107488621B (en) | 2021-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106635861B (en) | A kind of de- COD denitrification microorganism microbial inoculum of salt tolerant and preparation method thereof | |
| CN103374524B (en) | Salt-tolerant microbial agent and preparation method thereof | |
| CN105130011B (en) | Diatomite sewage-treating agent, preparation method and its technology application | |
| CN106906170A (en) | Complex micro organism fungicide and its preparation method and application | |
| CN109609406A (en) | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water | |
| CN106701633A (en) | Compound bactericide for treating sewage and sewage treatment method thereof | |
| CN107488621A (en) | A kind of denitrification denitrogenation composite bacteria agent and its preparation method and application | |
| CN105621626A (en) | High-concentration chemical sewage composite inocula and use thereof | |
| CN103374525B (en) | Wastewater treating microbial agent and preparation method thereof | |
| CN107699513B (en) | Black and odorous water body degrading bacterium and application thereof | |
| CN105132342A (en) | GS microbial inoculum for disposing life wastewater and application of GS microbial inoculum | |
| CN107236687A (en) | One plant have hexavalent chromium removal and aerobic denitrification capability concurrently Pseudomonas stutzeri and its application | |
| CN105296398A (en) | Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof | |
| CN105600945B (en) | A method of sewage is handled using composite flora | |
| CN102465102B (en) | Nitrous acid type biological denitrogenation microbial agent and application thereof | |
| CN105368737A (en) | Bacterial strain for treating high concentrated organic wastewater, microorganism bacterium agent and application thereof | |
| CN111440747B (en) | Compound microbial agent for sewage treatment, microecological preparation and application | |
| CN108118008A (en) | Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application | |
| CN107099483A (en) | A kind of composite biological agent and its application in processing mercury-containing waste water | |
| CN105969698A (en) | Composite microbial inoculant for water purification | |
| CN103381418B (en) | Method for processing tobacco waste or organic fluorine wastewater | |
| CN111072156A (en) | Composite microecological preparation for treating black and odorous water body | |
| CN107916242B (en) | Composite microbial inoculum for treating farmer sewage and preparation method thereof | |
| CN115960783A (en) | Anaerobic microorganism combination with sulfonamide antibiotic degradation function and application thereof | |
| CN106635860B (en) | A kind of Salt-tolerant microbial agent and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210709 Address after: No.6 zhongbing Road, West Industrial Cluster, zhongzhan District, Jiaozuo City, Henan Province Patentee after: Henan Zhongshun Environmental Protection Technology Co.,Ltd. Address before: No. 57, Jingxuan West Road, Jining City, Shandong Province Patentee before: QUFU NORMAL University |