CN107456424A

CN107456424A - A kind of nanoemulsion essence containing natural component extract and preparation method thereof

Info

Publication number: CN107456424A
Application number: CN201710693959.1A
Authority: CN
Inventors: 刘富岗
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-08-14
Filing date: 2017-08-14
Publication date: 2017-12-12

Abstract

The present invention relates to a kind of nanoemulsion essence containing natural component extract and preparation method thereof, fermentation technique is applied to the extraction of whitening moisturizing ingredient in the plant such as Chinese herbal medicine and natural flowers class, be advantageous to the Transdermal absorption of natural component, improve the skin whitening, moisturizing effect of natural component；Chinese medicine compound prescription fermentation polysaccharides will be extracted advantageously in the holding to skin moisture after the Chinese herb compound fermentation of scientific and reasonable formula；The grape-kernel oil naturally squeezed and vitamin E are applied to replace a part of oil phase in the oil phase of nanoemulsion essence, also largely improve whitening effect of the present invention；Inventive formulation is scientific and reasonable, simple for process, and effect is reliably definite, has very big Social benefit and economic benefit, is worthy to be popularized.

Description

A kind of nanoemulsion essence containing natural component extract and preparation method thereof

Technical field

The present invention relates to cosmetic field, more particularly to a kind of nanoemulsion essence containing natural component extract and Its preparation method.

Background technology

Chinese herbal medicine is with a history of thousands of years for treating and nursing various disease of skin, in recent years, with the height of economy Speed develops, and the people moisten pale and bright and clean skin and got over to living standard and to health and beautiful demand more and more higher Praised highly come more women, and ground for the toxicity to chemical toilet and irritating doubt, increasing cosmetics The person of studying carefully and manufacturer be more prone to using natural plants prepare safe and efficient, action temperature and, nontoxic non-stimulated natural beauty White moisture-keeping cosmetics.The skin whitening, moisturizing product of the thing containing plant component extraction in the market, has that powerful component is single, group Divide the defects of absorptivity is bad, skin whitening, moisturizing effect is bad, limit the development of natural whiting moisture-keeping cosmetics.

The content of the invention

For the above situation, do not solve the defect of prior art, present disclosure be just to provide it is a kind of containing naturally into Divide nanoemulsion essence of extract and preparation method thereof, can effectively solve toxicity, the excitant of existing skin whitening, moisturizing cosmetics And absorb the problem of bad effect is bad.

The technical scheme is that it is made up of the raw material of following percentage by weight meter：Soybean lecithin 1.14%- 1.68%th, Arabic gum 0.83%-2.56%, cithrol 0.34%-2.53%, tragacanth 0.27%- 0.64%th, peach gum 0.24%-0.46%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%-3.82%, citric acid 0.12%-0.71%, ethylene glycol 1.89%-3.77%, glycerine 2.46%-4.61%, vitamin E 0.06%-0.11%, grape Seed oil 0.66%-3.89%, olive oil 1.05%-2.58%, soybean oil 1.12%-3.31%, Chinese medicine compound prescription fermentate 0.96%-1.13%, Chinese medicine compound prescription fermentation polysaccharides 1.45%-1.67%, natural component fermentate 1.35%-1.73%, surplus For deionized water, total amount 100%；

A kind of preparation method of described nanoemulsion essence containing natural component extract, it is characterised in that including Following steps：

(1) citric acid, Arabic gum, tragacanth, the peach gum of recipe quantity are taken, is well mixed, adds citric acid, Arab The deionized water dissolving that glue, tragacanth, 6.8-7.5 times of peach gum gross weight are measured, sequentially add the soybean lecithin of recipe quantity, poly- second Diol fatty acid ester, polyoxyethylene (20) sorbitan mono-oleic acid ester, it is well mixed, sequentially adds the grape pip of recipe quantity Oil, olive oil, soybean oil, vitamin E, ethylene glycol, glycerine, it is well mixed, obtains nanoemulsion essence matrix；

(2) the Chinese medicine compound prescription fermentate, Chinese medicine compound prescription fermentation polysaccharides, natural component fermentate of recipe quantity are taken, is well mixed, Add Chinese medicine compound prescription fermentate, Chinese medicine compound prescription fermentation polysaccharides, 62 DEG C of 6.1 times of amounts of natural component fermentate gross weight go from Sub- water makes dissolving, puts to room temperature, obtains natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 41 DEG C -52 DEG C, then stirred 47 DEG C of deionized water is slowly added in the case of mixing, until adding the deionized water of surplus, is well mixed, puts to room temperature, Obtain the described nanoemulsion essence containing natural component extract.

Compatibility of the present invention is reasonable, definite effect, fermentation technique is used for the preparation of Chinese herbal medicine and natural plant extracts, more Beneficial to absorbing for natural component, and the product that nano-emulsion type is made more is advantageous to play whitening U.S. of natural constituents White moisture-keeping efficacy, be advantageous to the Transdermal absorption of component, manufacture craft simple possible, economic benefit and social benefit are notable, are days Huge innovation on right composition cosmetics.

Embodiment

The embodiment of the present invention is elaborated below in conjunction with example.

A kind of nanoemulsion essence containing natural component extract of the present invention by following percentage by weight meter raw material system Into：Soybean lecithin 1.14%-1.68%, Arabic gum 0.83%-2.56%, cithrol 0.34%-2.53%, Tragacanth 0.27%-0.64%, peach gum 0.24%-0.46%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%- 3.82%th, citric acid 0.12%-0.71%, ethylene glycol 1.89%-3.77%, glycerine 2.46%-4.61%, vitamin E0.06%-0.11%, grape-kernel oil 0.66%-3.89%, olive oil 1.05%-2.58%, soybean oil 1.12%-3.31%, Chinese medicine compound prescription fermentate 0.96%-1.13%, Chinese medicine compound prescription fermentation polysaccharides 1.45%-1.67%, natural component fermentate 1.35%-1.73%, surplus are deionized water, total amount 100%；

The preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, cape jasmine 1.5 parts, 1.2 parts of the bletilla striata, 1 part of kuh-seng, be well mixed, be ground into coarse powder, be placed in flash extracter and extract 3 times, every time addition The bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 9.3-27.6 times of Sophora flavescens gross weight 45.6% ethanol (V/V) measured, when extracting every time Between be 1.4min-4.8min, filtering, filter residue a is standby, merging filtrate, and 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.12-1.23 Medicinal extract, 5500r/min centrifugation 15min, take the supernatant lactobacillus that has activated of access or one or both of saccharomycete Arbitrary proportion mixes, and inoculum concentration is 0.56%-22.8% volumes, and fermentation temperature is 27.8 DEG C -46.1 DEG C, and fermentation pH is 4.4- 6.7, fermentation time 27h-46h, after the completion of fermentation 79 DEG C -121 DEG C sterilizing 17min-61min, filtering, filtrate 5600r/min from Heart 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter residue a adds the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, hardship Join medicinal material 8.5-17.6 times of deionized water measured of gross weight, 87.8 DEG C of -98.5 DEG C of refluxing extraction 64min-115min, filter, filter Proportion is 1.13-1.21 medicinal extract when liquid is concentrated under reduced pressure into 40 DEG C, and 5400r/min centrifugation 16min, supernatant, which adds ethanol, to be made to contain Alcohol amount is up to 81.2% (V/V), and 4 DEG C of standing 8h-24h, 5700r/min centrifugation 15min remove supernatant, with 11 times of weight of precipitation Absolute ethyl alcohol washs, and 5300r/min centrifugations 10min removes supernatant, and lower sediment sevage methods take off albumen, be freeze-dried in Medicine compound fermentation polysaccharide；Described natural component fermentate its preparation method is：Take 2.5 parts of Flos Magnoliae Officinalis in parts by weight, people Ginseng spend 2 parts, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, 2.2 parts of sanchi flower, be well mixed, Fine powder is ground into, is placed in flash extracter and extracts 2 times, add Flos Magnoliae Officinalis, flower of Panax ginseng, flower of Radix Puerariae, Astragalis flower, okra every time Flower, rose, 6.6-18.7 times of sanchi flower total weight 47% ethanol (V/V) measured, each extraction time is 1.3min- 2.7min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.06-1.17,5700r/min centrifugations 18min, the one or more of arbitrary proportions mixing for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated, Inoculum concentration is 0.21%-18.9% volumes, and fermentation temperature is 28.4 DEG C -45.7 DEG C, and fermentation pH is 4.5-6.9, fermentation time 21h-38h, 83 DEG C of -122 DEG C of sterilizing 18min-70min after the completion of fermentation, filtering, filtrate 5800r/min centrifugation 15.9min, on Solvent is recovered under reduced pressure in 47 DEG C of clear liquid, and proportion is 1.13-1.19 medicinal extract when being condensed into 40 DEG C, is freeze-dried to obtain natural component fermentation Thing；Described grape-kernel oil is made by milling process.

Recorded according to pharmacopeia：Described coarse powder is mixed with for that all can be sieved by No. two and can be no more than 40% by No. four sieves Powder；Described fine powder for all by No. five sieve, and containing can pass through No. six sieve be no less than 95% powder；No. two sieves It it is 850 μm ± 29 μm for sieve aperture internal diameter (average value), mesh number is 24 mesh；No. four sieves are that sieve aperture internal diameter (average value) is 250 μ M ± 9.9 μm, mesh number are 65 mesh；No. five sieves are that sieve aperture internal diameter (average value) is 180 μm ± 7.6 μm, and mesh number is 80 mesh；Institute It is that sieve aperture internal diameter (average value) is 150 μm ± 6.6 μm to state No. six sieves, and mesh number is 100 mesh；

The present invention can be realized by following examples.

Embodiment 1

The present invention is made up of the raw material of following percentage by weight meter：Soybean lecithin 1.14%, Arabic gum 0.83%, poly- second Diol fatty acid ester 0.34%, tragacanth 0.27%, peach gum 0.24%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%th, citric acid 0.12%, ethylene glycol 1.89%, glycerine 2.46%, vitamin E 0.06%, grape-kernel oil 0.66%, olive Oil 1.05%, soybean oil 1.12%, Chinese medicine compound prescription fermentate 0.96%, Chinese medicine compound prescription fermentation polysaccharides 1.45%, natural component hair Ferment thing 1.35%, deionized water 85.33%；

The preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, cape jasmine 1.5 parts, 1.2 parts of the bletilla striata, 1 part of kuh-seng, be well mixed, be ground into coarse powder, be placed in flash extracter and extract 3 times, every time addition The bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 45.6% ethanol (V/V) of 9.3 times of Sophora flavescens gross weight amounts, each extraction time be 1.4min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.12,5500r/min 15min is centrifuged, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, inoculation It is 27.8 DEG C to measure as 0.56% volume, fermentation temperature, and fermentation pH is 4.4, fermentation time 27h, 79 DEG C of sterilizings after the completion of fermentation 17min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter residue a The addition bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized waters of 8.5 times of amounts of Sophora flavescens gross weight, 87.8 DEG C of refluxing extraction 64min, Filtering, proportion is 1.13 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifugation 16min, supernatant, which adds ethanol, to be made Alcohol content removes supernatant, 11 times of precipitation of lower sediment up to 81.2% (V/V), 4 DEG C of standing 8h, 5700r/min centrifugation 15min The absolute ethyl alcohol washing of weight, 5300r/min centrifugations 10min remove supernatant, and lower sediment sevage methods take off albumen, and freezing is dry It is dry to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take Flos Magnoliae Officinalis 2.5 in parts by weight Part, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, 2.2 parts of sanchi flower, mixing Uniformly, be ground into fine powder, be placed in flash extracter and extract 2 times, add every time Flos Magnoliae Officinalis, flower of Panax ginseng, flower of Radix Puerariae, Astragalis flower, Okra, rose, 47% ethanol (V/V) of 6.6 times of sanchi flower total weight amounts, each extraction time are 1.3min, mistake Filter, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.06,5700r/min centrifugation 18min, take supernatant The one or more of arbitrary proportion mixing accessed in lactic acid bacteria, Aspergillus or the saccharomycete activated, inoculum concentration 0.21% Volume, fermentation temperature are 28.4 DEG C, and fermentation pH is 4.5, fermentation time 21h, and 83 DEG C of sterilizing 18min, are filtered after the completion of fermentation, filter Liquid 5800r/min centrifuges 15.9min, and solvent is recovered under reduced pressure in 47 DEG C of supernatant, and proportion is 1.13 medicinal extract when being condensed into 40 DEG C, cold Lyophilized dry natural component fermentate；Described grape-kernel oil is made by milling process.

Embodiment 2

The present invention is made up of the raw material of following percentage by weight meter：Soybean lecithin 1.68%, Arabic gum 2.56%, poly- second Diol fatty acid ester 2.53%, tragacanth 0.64%, peach gum 0.46%, polyoxyethylene (20) sorbitan mono-oleic acid ester 3.82%th, citric acid 0.71%, ethylene glycol 3.77%, glycerine 4.61%, vitamin E 0.11%, grape-kernel oil 3.89%, olive Oil 2.58%, soybean oil 3.31%, Chinese medicine compound prescription fermentate 1.13%, Chinese medicine compound prescription fermentation polysaccharides 1.67%, natural component hair Ferment thing 1.73%, deionized water 64.8%；

The preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, cape jasmine 1.5 parts, 1.2 parts of the bletilla striata, 1 part of kuh-seng, be well mixed, be ground into coarse powder, be placed in flash extracter and extract 3 times, every time addition The bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 45.6% ethanol (V/V) of 27.6 times of Sophora flavescens gross weight amounts, each extraction time be 4.8min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.23,5500r/min 15min is centrifuged, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, inoculation It is 46.1 DEG C to measure as 22.8% volume, fermentation temperature, and fermentation pH is 6.7, fermentation time 46h, 121 DEG C of sterilizings after the completion of fermentation 61min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter residue a Add the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized water of 17.6 times of amounts of Sophora flavescens gross weight, 98.5 DEG C of refluxing extractions 115min, filtering, proportion is 1.21 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifuges 16min, and supernatant adds Entering ethanol makes alcohol content remove supernatant, lower sediment up to 81.2% (V/V), 4 DEG C of standing 24h, 5700r/min centrifugation 15min Washed with the absolute ethyl alcohol of 11 times of weight of precipitation, 5300r/min centrifugations 10min removes supernatant, and lower sediment sevage methods take off Albumen, it is freeze-dried to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take in parts by weight 2.5 parts of Flos Magnoliae Officinalis, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, pseudo-ginseng 2.2 parts are spent, is well mixed, is ground into fine powder, is placed in flash extracter and extracts 2 times, add Flos Magnoliae Officinalis, flower of Panax ginseng, Pueraria lobota every time Root flower, Astragalis flower, okra, rose, 18.7 times of 47% ethanol (V/V) measured of sanchi flower total weight, when extracting every time Between be 2.7min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.17,5700r/min centrifugations 18min, the one or more of arbitrary proportions mixing for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated, Inoculum concentration is 18.9% volume, and fermentation temperature is 45.7 DEG C, and fermentation pH is 6.9, fermentation time 38h, and 122 DEG C go out after the completion of fermentation Solvent is recovered under reduced pressure in bacterium 70min, filtering, filtrate 5800r/min centrifugation 15.9min, 47 DEG C of supernatant, proportion when being condensed into 40 DEG C For 1.19 medicinal extract, natural component fermentate is freeze-dried to obtain；Described grape-kernel oil is made by milling process.

Embodiment 3

The present invention is made up of the raw material of following percentage by weight meter：Soybean lecithin 1.45%, Arabic gum 1.75%, poly- second Diol fatty acid ester 1.42%, tragacanth 0.50%, peach gum 0.35%, polyoxyethylene (20) sorbitan mono-oleic acid ester 1.45%th, citric acid 0.42%, ethylene glycol 2.75%, glycerine 3.57%, vitamin E 0.08%, grape-kernel oil 2.76%, olive Oil 1.81%, soybean oil 2.24%, Chinese medicine compound prescription fermentate 1.05%, Chinese medicine compound prescription fermentation polysaccharides 1.56%, natural component hair Ferment thing 1.55%, deionized water 75.29%；

The preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, cape jasmine 1.5 parts, 1.2 parts of the bletilla striata, 1 part of kuh-seng, be well mixed, be ground into coarse powder, be placed in flash extracter and extract 3 times, every time addition The bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 45.6% ethanol (V/V) of 18.7 times of Sophora flavescens gross weight amounts, each extraction time be 3.5min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.17,5500r/min 15min is centrifuged, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, inoculation It is 36.6 DEG C to measure as 0.56%-22.8% volumes, fermentation temperature, and fermentation pH is 5.5, fermentation time 36h, 102 after the completion of fermentation DEG C sterilizing 39min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant is dried under reduced pressure, and obtains Chinese medicine compound prescription fermentation Thing；Filter residue a adds the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized water of 13.2 times of amounts of Sophora flavescens gross weight, and 92.2 DEG C of backflows carry 88min is taken, is filtered, proportion is 1.17 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifuges 16min, and supernatant adds Entering ethanol makes alcohol content remove supernatant, lower sediment up to 81.2% (V/V), 4 DEG C of standing 16h, 5700r/min centrifugation 15min Washed with the absolute ethyl alcohol of 11 times of weight of precipitation, 5300r/min centrifugations 10min removes supernatant, and lower sediment sevage methods take off Albumen, it is freeze-dried to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take in parts by weight 2.5 parts of Flos Magnoliae Officinalis, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, pseudo-ginseng 2.2 parts are spent, is well mixed, is ground into fine powder, is placed in flash extracter and extracts 2 times, add Flos Magnoliae Officinalis, flower of Panax ginseng, Pueraria lobota every time Root flower, Astragalis flower, okra, rose, 12.7 times of 47% ethanol (V/V) measured of sanchi flower total weight, when extracting every time Between be 2.0min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.11,5700r/min centrifugations 18min, the one or more of arbitrary proportions mixing for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated, Inoculum concentration is 9.1% volume, and fermentation temperature is 36.8 DEG C, and fermentation pH is 5.7, fermentation time 29.5h, 103 DEG C after the completion of fermentation Sterilize 44min, filtering, and filtrate 5800r/min centrifugation 15.9min, solvent is recovered under reduced pressure in 47 DEG C of supernatant, be condensed into 40 DEG C when ratio Weight is 1.16 medicinal extract, is freeze-dried to obtain natural component fermentate；Described grape-kernel oil is made by milling process.

Embodiment 4

The present invention is made up of the raw material of following percentage by weight meter：Soybean lecithin 1.14%, Arabic gum 2.56%, poly- second Diol fatty acid ester 1.42%, tragacanth 0.64%, peach gum 0.35%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%th, citric acid 0.12%, ethylene glycol 1.89%, glycerine 4.61%, vitamin E 0.11%, grape-kernel oil 2.76%, olive Oil 1.81%, soybean oil 3.31%, Chinese medicine compound prescription fermentate 0.96%, Chinese medicine compound prescription fermentation polysaccharides 1.67%, natural component hair Ferment thing 1.55%, deionized water 74.37%；

The preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, cape jasmine 1.5 parts, 1.2 parts of the bletilla striata, 1 part of kuh-seng, be well mixed, be ground into coarse powder, be placed in flash extracter and extract 3 times, every time addition The bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 45.6% ethanol (V/V) of 9.3 times of Sophora flavescens gross weight amounts, each extraction time be 3.5min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.23,5500r/min 15min is centrifuged, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, inoculation It is 27.8 DEG C to measure as 22.8% volume, fermentation temperature, and fermentation pH is 4.4, fermentation time 46h, 102 DEG C of sterilizings after the completion of fermentation 17min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter residue a The addition bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized waters of 8.5 times of amounts of Sophora flavescens gross weight, 92.2 DEG C of refluxing extraction 88min, Filtering, proportion is 1.13 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifugation 16min, supernatant, which adds ethanol, to be made Alcohol content removes supernatant, lower sediment precipitation 11 up to 81.2% (V/V), 4 DEG C of standing 24h, 5700r/min centrifugation 15min The absolute ethyl alcohol washing of times weight, 5300r/min centrifugations 10min remove supernatant, and lower sediment sevage methods take off albumen, freezing Dry Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take Flos Magnoliae Officinalis in parts by weight 2.5 parts, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, 2.2 parts of sanchi flower, It is well mixed, fine powder is ground into, is placed in flash extracter and extracts 2 times, add Flos Magnoliae Officinalis, flower of Panax ginseng, flower of Radix Puerariae, the Radix Astragali every time Flower, okra, rose, 47% ethanol (V/V) of 18.7 times of sanchi flower total weight amounts, each extraction time be 1.3min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.11,5700r/min centrifugation 18min, The one or more of arbitrary proportions mixing for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated, inoculum concentration For 18.9% volume, fermentation temperature is 28.4 DEG C, and fermentation pH is 5.7, fermentation time 29.5h, 83 DEG C of sterilizings after the completion of fermentation Solvent is recovered under reduced pressure in 18min, filtering, filtrate 5800r/min centrifugation 15.9min, 47 DEG C of supernatant, and proportion is when being condensed into 40 DEG C 1.19 medicinal extract, it is freeze-dried to obtain natural component fermentate；Described grape-kernel oil is made by milling process.

Embodiment 5

The preparation method of nanoemulsion essence of the invention containing natural component extract, comprises the following steps：

(1) citric acid 0.12kg, Arabic gum 0.83kg, tragacanth 0.27kg, peach gum 0.24kg are taken, is well mixed, Deionized water 9.93kg dissolvings are added, then sequentially add soybean lecithin 1.14kg, the cithrol of recipe quantity 0.34kg, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73kg, it is well mixed, sequentially adds the grape-kernel oil of recipe quantity 0.66kg, olive oil 1.05kg, soybean oil 1.12kg, vitamin E 0.06kg, ethylene glycol 1.89kg, glycerine 2.46kg, mixing are equal It is even, obtain nanoemulsion essence matrix；

(2) Chinese medicine compound prescription fermentate 0.96kg, Chinese medicine compound prescription fermentation polysaccharides 1.45kg, natural component fermentate are taken 1.35kg, it is well mixed, adding 62 DEG C of deionized water 22.56kg makes dissolving, puts to room temperature, it is molten to obtain natural component extract Liquid；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 41 DEG C, then in stirring In the case of be slowly added to 47 DEG C of deionized water 52.84kg, be well mixed, put to room temperature, that is, obtain it is described containing naturally into Divide the nanoemulsion essence of extract.

Embodiment 6

The preparation method of nanoemulsion essence of the invention containing natural component extract, it is characterised in that including following Step：

(1) citric acid 0.71kg, Arabic gum 2.56kg, tragacanth 0.64kg, peach gum 0.46kg are taken, is well mixed, Deionized water 32.78kg dissolvings are added, sequentially add soybean lecithin 1.68kg, the cithrol of recipe quantity 2.53kg, polyoxyethylene (20) sorbitan mono-oleic acid ester 3.82kg, it is well mixed, sequentially adds the grape-kernel oil of recipe quantity 3.89kg, olive oil 2.58kg, soybean oil 3.31kg, vitamin E 0.11kg, ethylene glycol 3.77kg, glycerine 4.61kg, mixing are equal It is even, obtain nanoemulsion essence matrix；

(2) Chinese medicine compound prescription fermentate 1.13kg, Chinese medicine compound prescription fermentation polysaccharides 1.67kg, natural component fermentate are taken 1.73kg, it is well mixed, adding 62 DEG C of deionized water 39.83kg makes dissolving, puts to room temperature, it is molten to obtain natural component extract Liquid；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 52 DEG C, then in stirring In the case of be slowly added to 47 DEG C of deionized water 1.54kg, be well mixed, put to room temperature, that is, obtain described containing natural component The nanoemulsion essence of extract.

Embodiment 7

(1) citric acid 0.42kg, Arabic gum 1.75kg, tragacanth 0.50kg, peach gum 0.35kg are taken, is well mixed, Adding deionized water 21.59kg (7.15 times) makes dissolving, sequentially adds soybean lecithin 1.45kg, cithrol 1.42kg, polyoxyethylene (20) sorbitan mono-oleic acid ester 1.45kg, it is well mixed, sequentially adds grape-kernel oil 2.76kg, olive Olive oil 1.81kg, soybean oil 2.24kg, vitamin E 0.08, ethylene glycol 2.75kg, glycerine 3.57kg, it is well mixed, obtains nano-emulsion Essence matrix；

(2) Chinese medicine compound prescription fermentate 1.05kg, Chinese medicine compound prescription fermentation polysaccharides 1.56kg, natural component fermentate are taken 1.55kg, it is well mixed, adding 62 DEG C of deionized water 25.38kg makes dissolving, and solution is put to room temperature, and it is molten to obtain natural component extract Liquid；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 46 DEG C, then in stirring In the case of be slowly added to 47 DEG C of deionized water 28.23kg, be well mixed, put to room temperature, that is, obtain it is described containing naturally into Divide the nanoemulsion essence of extract.

Embodiment 8

(1) citric acid 0.12kg, Arabic gum 2.56kg, tragacanth 0.64kg, peach gum 0.35kg are taken, is well mixed, Adding deionized water 24.96kg makes dissolving, sequentially adds soybean lecithin 1.14kg, cithrol 1.42kg, polyoxy Ethene (20) sorbitan mono-oleic acid ester 0.73kg, it is well mixed, sequentially adds grape-kernel oil 2.76kg, the olive oil of recipe quantity 1.81kg, soybean oil 3.31kg, vitamin E 0.11kg, ethylene glycol 1.89kg, glycerine 4.61kg, it is well mixed, obtains a nanometer milk extract Magnificent liquid-based matter；

(2) Chinese medicine compound prescription fermentate 0.96kg, Chinese medicine compound prescription fermentation polysaccharides 1.67kg, natural component fermentate are taken 1.55kg, it is well mixed, adding 62 DEG C of deionized water 25.50kg makes dissolving, puts to room temperature, obtains natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 41 DEG C, then in stirring In the case of be slowly added to 47 DEG C of deionized water 23.91kg, be well mixed, put to room temperature, that is, obtain it is described containing naturally into Divide the nanoemulsion essence of extract.

Above-mentioned is only to provide several examples, and in development, inventor achieves same or similar through repeated multiple times experiment Result, methodological science is effective, has higher actual application value, and to nano-emulsion of the present invention containing natural component extract The effects such as the whitening of Essence, moisturizing carry out repetition test and achieve satisfied technique effect, and relevant testing data is as follows：

First, Efficiency assessment

A. whitening effect determines

Day is contained to measure the present invention using colorimeter system (Lab colorimeter systems) as defined in International Commission on Illumination (CIE) The change of skin color before and after the nanoemulsion essence effect of right component extract, evaluate the whitening effect of the present invention.

As a result show, after product of the present invention 6 months, colour difference meter detection use before and after change of skin, as a result find with Control skin is compared, and unfolds wrinkle using this product, and skin whitening effects improve.

B. the nanoemulsion essence of the invention containing natural component extract is to tyrosinase inhibitory activity

Tyrosinase is the crucial rate-limiting enzyme that melanin is formed, and is commented by determining inhibitor to the inhibiting rate of tyrosinase Valency whitening effect, its principle are：Tyrosinase can tyrosine generation dopachrome in catalytic machine body, it is in ultraviolet specrophotometer There is absorption maximum at 475nm, the amount that dopachrome can be generated by determining is active to calculate tyrosinase inhibitor.This method with Tyrosine is substrate, and active skull cap components are examined to tyrosinase activity inhibitory action by chemical method, easy to operate, straight See, detected suitable for initial whitening effect.

Tyrosinase vitality test principle：During tyrosinase reacts generation melanin, the catalysis of tyrosinase Effect, which occurs mainly in, makes that tyrosine is converted into DOPA and DOPA is converted into DOPA quinone this two-step reaction.DOPA quinone is a kind of coloured Material, there is absorption maximum at 475nm, spectrophotometric determination can be used.The definition of tyrosinase vigor：With DOPA per minute Absorbance increase by 0.001 of the quinone at 475nm is an enzyme activity unit, i.e. 1.0U/min.

Solution with phosphate buffer by sample preparation into various concentrations, using 2.5mmol/L TYRs solution the bottom of as Thing, in 3.0mL measure systems, first add 0.5mL substrates, the sample solution and 0.2mol/L phosphate buffers of different volumes (pH=6.8) (PBS), 10min is incubated in 37 DEG C of thermostat water baths, then adds 0.5mL117.53U/mL junket ammonia Sour enzyme solutions, are mixed immediately, and 30min is reacted in 37 DEG C of thermostat water baths, determine the absorbance (A) at wavelength 475nm immediately.

Tyrosinase relative activity inhibiting rate is calculated according to the absorbance measured, tyrosinase suppression is calculated by formula below Rate (I) processed：

In formula, A₀To contain substrate, the absorbance of the live body system without tyrosinase and sample；A₁For containing substrate and Tyrosinase, the absorbance of the live body system without sample；A₂For containing substrate and sample, the live body system without tyrosinase Absorbance；A₃For the absorbance of the live body system containing substrate, tyrosinase and sample.

Using kojic acid as positive control, product of the present invention is shown in Table 1 to the inhibitory action experimental data of tyrosinase.

Inhibitory action of the nanoemulsion essence of the invention containing natural component extract of table 1 to tyrosinase

As shown in Table 1, the nanoemulsion essence of the invention containing natural component extract has certain to tyrosinase Inhibitory action, its action intensity are slightly below kojic acid, to the inhibition of tyrosinase monophenolase activity apparently higher than tyrosinase Bis-phenol enzyme.

It is 71.4% to tyrosinase bis-phenol enzyme inhibition rate when product design of the present invention is 5.0mg/mL；When the present invention produces Product concentration is 10mg/mL, is 89.8% to tyrosinase bis-phenol enzyme inhibition rate；When product design of the present invention is 20mg/mL, to junket Propylhomoserin enzyme bis-phenol enzyme inhibition rate is 95.1%, and as can be seen from the above results, with the increasing of product design of the present invention, it suppresses The activity of tyrosinase is stronger.

Remarks explanation:The nanoemulsion essence concentration that product of the present invention contains natural component extract is 5.0mg/mL, i.e., Refer to and 5.0mg additions lmL deionized waters are diluted gained；Remaining is by that analogy.

C. nanoemulsion essence water conservation humectant properties evaluation of the present invention containing natural component extract

Carry out moistening effect test (with COrneometer methods test moistening effect) to the batch of product 8 of the present invention, every batch The secondary people of women on probation 15, test result are as follows:

The nanoemulsion essence water conservation moisturizing result of the test of the invention containing natural component extract of table 2

As a result show, the nanoemulsion essence of the invention containing natural component extract is substantially increased with rear skin moisture content Add, moistening effect is persistently obvious.

Investigate the nanoemulsion essence of the invention containing natural component extract and epidermal cell (HaCat) is immortalized to people Effect, with skin aquaporin before and after nanoemulsion essence effect of the Western blot present invention containing natural component extract Albumen 1 (AQP3) changes, and analyzes tune of the nanoemulsion essence of the invention containing natural component extract to aquaporin Section acts on, from molecular biology angle illustrate the nanoemulsion essence containing natural component extract of the invention moistening effect and Mechanism.

As a result show, product of the present invention can promote the horizontal expression of aquaporin -3 (AQP-3) well, so as to from point Sub- biology angle illustrates that the nanoemulsion essence of the invention containing natural component extract has water conservation moisture-keeping function.

2nd, the nanoemulsion essence safety evaluatio of the invention containing natural component extract

A. skin allergy test

Draize White Rabbit models:By White Rabbit 3, back backbone diamond wool is cut, and does not damage epidermis, unhairing scope Left and right each about 5cm × 5cm.Tested material 0.5g is coated directly on the skin of left side, is covered with gauze, is finally consolidated with adhesive plaster Fixed, right side skin application time 72h, observes skin lh, 24h, 48h, 72h reactions respectively as control.Press《European Union's cosmetics Method for evaluating safety》Cutaneous sensibility evaluation is carried out, has checked whether that erythema and oedema occur.

Human skin patch:After Skin stimulant experiment in Rabbit is carried out, continue human skin patch. The people of volunteer 15 is chosen, tested material 0.5g is evenly coated on external application sticker, spreads on the right forearm crook that volunteer cleans, is observed 24h inner skin response situations.

White Rabbit and human skin patch result show that this product does not cause any stimulate the reaction.It follows that this Invention product has higher safety in utilization, to human body skin without any adverse reaction.It the results are shown in Table 2.

2 product of the present invention of table is test White Rabbit cutaneous sensibility

Note：PD II is worth+3 rabbit average marks of (skin irritation index)=erythema average mark

After human body skin patch result shows product effect of the present invention, any stimulate the reaction is not caused.Pass through this experiment As a result it can show that the security of product of the present invention is all very high, adverse reaction is not caused to human body skin.

Above-mentioned result of the test shows that the nanoemulsion essence of the invention containing natural component extract is safe and reliable, Do not occur the ill symptomses such as redness, pruitus, allergy, safe without toxic side effect, there is good skin whitening, moisturizing effect, have wide General application value.

The present invention has advantages below compared with prior art：Develop a kind of safe and efficient skin whitening, moisturizing of natural green Nanoemulsion essence cosmetics, fermentation technique is applied to whitening moisturizing ingredient in the plant such as Chinese herbal medicine and natural flowers class and carried Take, advantageously improve the skin whitening, moisturizing effect of composition in the Transdermal absorption of natural component；By the medium-height grass of scientific and reasonable formula Chinese medicine compound prescription fermentation polysaccharides are extracted after medicine compound fermentation advantageously in the holding to skin moisture；The grape pip that will naturally squeeze Oil and vitamin E are applied to replace a part of oil phase in the oil phase of nanoemulsion essence, also largely improve this hair Bright whitening effect；Inventive formulation is scientific and reasonable, simple for process, and effect is reliably definite, have very big social benefit and Economic benefit, it is the huge innovation on natural component cosmetics, there is larger promotional value.

Claims

1. a kind of nanoemulsion essence containing natural component extract, it is characterised in that by the original of following percentage by weight meter Material is made：Soybean lecithin 1.14%-1.68%, Arabic gum 0.83%-2.56%, cithrol 0.34%- 2.53%th, tragacanth 0.27%-0.64%, peach gum 0.24%-0.46%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%-3.82%, citric acid 0.12%-0.71%, ethylene glycol 1.89%-3.77%, glycerine 2.46%-4.61%, dimension life Plain E0.06%-0.11%, grape-kernel oil 0.66%-3.89%, olive oil 1.05%-2.58%, soybean oil 1.12%- 3.31%th, Chinese medicine compound prescription fermentate 0.96%-1.13%, Chinese medicine compound prescription fermentation polysaccharides 1.45%-1.67%, natural component fermentation Thing 1.35%-1.73%, surplus are deionized water, total amount 100%；Wherein, the preparation side of described Chinese medicine compound prescription fermentate Method is：2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, 1.5 parts of cape jasmine, 1.2 parts of the bletilla striata, 1 part of kuh-seng are taken, is well mixed, powder Coarse powder is broken into, is placed in flash extracter and extracts 3 times, every time adds the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, Sophora flavescens gross weight 9.3-27.6 times of 45.6% ethanol (V/V) measured, each extraction time are 1.4min-4.8min, and filtering, filter residue a is standby, are merged Filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.12-1.23,5500r/min centrifugation 15min, supernatant is taken to connect Enter the arbitrary proportion mixing of the lactobacillus activated or one or both of saccharomycete, inoculum concentration is 0.56%-22.8% bodies Product, fermentation temperature are 27.8 DEG C -46.1 DEG C, and fermentation pH is 4.4-6.7, fermentation time 27h-46h, 79 DEG C -121 after the completion of fermentation DEG C sterilizing 17min-61min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant is dried under reduced pressure, and obtains Chinese medicine compound prescription Fermentate；The deionized water that the filter residue a additions bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 8.5-17.6 times of Sophora flavescens gross weight are measured, 87.8 DEG C -98.5 DEG C of refluxing extraction 64min-115min, filtering, proportion is 1.13-1.21 medicinal extract when filtrate decompression is concentrated into 40 DEG C, 5400r/min centrifuges 16min, and supernatant, which adds ethanol, makes alcohol content up to 81.2% (V/V), 4 DEG C of standings 8h-24h, 5700r/ Min centrifugations 15min removes supernatant, is washed with the absolute ethyl alcohol of 11 times of weight of precipitation, in 5300r/min centrifugation 10min removings Clear liquid, lower sediment sevage methods take off albumen, are freeze-dried to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its Preparation method is：Take 2.5 parts of Flos Magnoliae Officinalis in parts by weight, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, okra 1.6 parts, 2.3 parts of rose, 2.2 parts of sanchi flower, be well mixed, be ground into fine powder, be placed in flash extracter and extract 2 times, often It is secondary addition Flos Magnoliae Officinalis, flower of Panax ginseng, flower of Radix Puerariae, Astragalis flower, okra, rose, 6.6-18.7 times of sanchi flower total weight measure 47% ethanol (V/V), each extraction time is 1.3min-2.7min, filtering, merging filtrate, 43 DEG C are concentrated under reduced pressure into 40 DEG C When proportion be 1.06-1.17 medicinal extract, 5700r/min centrifugation 18min, take supernatant access activated lactic acid bacteria, Aspergillus Or one or more of arbitrary proportions mixing in saccharomycete, inoculum concentration are 0.21%-18.9% volumes, fermentation temperature 28.4 DEG C -45.7 DEG C, fermentation pH is 4.5-6.9, fermentation time 21h-38h, 83 DEG C of -122 DEG C of sterilizing 18min- after the completion of fermentation Solvent is recovered under reduced pressure in 70min, filtering, filtrate 5800r/min centrifugation 15.9min, 47 DEG C of supernatant, and proportion is when being condensed into 40 DEG C 1.13-1.19 medicinal extract, be freeze-dried to obtain natural component fermentate；Described grape-kernel oil is made by milling process.

2. the preparation method of the nanoemulsion essence according to claim 1 containing natural component extract, its feature exist In comprising the following steps：

(1) citric acid, Arabic gum, tragacanth, the peach gum of recipe quantity are taken, be well mixed, add citric acid, Arabic gum, The deionized water dissolving that tragacanth, 6.8-7.5 times of peach gum gross weight are measured, sequentially add soybean lecithin, the poly- second two of recipe quantity Alcohol fatty acid ester, polyoxyethylene (20) sorbitan mono-oleic acid ester, be well mixed, sequentially add recipe quantity grape-kernel oil, Olive oil, soybean oil, vitamin E, ethylene glycol, glycerine, it is well mixed, obtains nanoemulsion essence matrix；

(2) the Chinese medicine compound prescription fermentate, Chinese medicine compound prescription fermentation polysaccharides, natural component fermentate of recipe quantity are taken, is well mixed, is added Chinese medicine compound prescription fermentate, Chinese medicine compound prescription fermentation polysaccharides, 6.1 times of 62 DEG C of deionized waters measured of natural component fermentate gross weight Make dissolving, put to room temperature, obtain natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 41 DEG C -52 DEG C, then in stirring In the case of be slowly added to 47 DEG C of deionized water, until adding the deionized water of surplus, be well mixed, put to room temperature, produce To the nanoemulsion essence containing natural component extract.

3. the nanoemulsion essence according to claim 1 containing natural component extract, it is characterised in that by following heavy The raw material of amount percentages is made：Soybean lecithin 1.14%, Arabic gum 0.83%, cithrol 0.34%, west Bassora gum 0.27%, peach gum 0.24%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%, citric acid 0.12%, second two Alcohol 1.89%, glycerine 2.46%, vitamin E 0.06%, grape-kernel oil 0.66%, olive oil 1.05%, soybean oil 1.12%, in Medicine compound fermentation thing 0.96%, Chinese medicine compound prescription fermentation polysaccharides 1.45%, natural component fermentate 1.35%, deionized water 85.33%；

Wherein, the preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, Cape jasmine 1.5 parts of son, 1.2 parts of the bletilla striata, 1 part of kuh-seng, it is well mixed, is ground into coarse powder, is placed in flash extracter and extracts 3 times, adds every time Enter 45.6% ethanol (V/V) of the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 9.3 times of Sophora flavescens gross weight amounts, each extraction time is 1.4min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.12,5500r/min 15min is centrifuged, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, inoculation It is 27.8 DEG C to measure as 0.56% volume, fermentation temperature, and fermentation pH is 4.4, fermentation time 27h, 79 DEG C of sterilizings after the completion of fermentation 17min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter residue a The addition bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized waters of 8.5 times of amounts of Sophora flavescens gross weight, 87.8 DEG C of refluxing extraction 64min, Filtering, proportion is 1.13 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifugation 16min, supernatant, which adds ethanol, to be made Alcohol content removes supernatant, 11 times of precipitation of lower sediment up to 81.2% (V/V), 4 DEG C of standing 8h, 5700r/min centrifugation 15min The absolute ethyl alcohol washing of weight, 5300r/min centrifugations 10min remove supernatant, and lower sediment sevage methods take off albumen, and freezing is dry It is dry to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take Flos Magnoliae Officinalis 2.5 in parts by weight Part, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, 2.2 parts of sanchi flower, mixing Uniformly, be ground into fine powder, be placed in flash extracter and extract 2 times, add every time Flos Magnoliae Officinalis, flower of Panax ginseng, flower of Radix Puerariae, Astragalis flower, Okra, rose, 47% ethanol (V/V) of 6.6 times of sanchi flower total weight amounts, each extraction time are 1.3min, mistake Filter, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.06,5700r/min centrifugation 18min, take supernatant The one or more of arbitrary proportion mixing accessed in lactic acid bacteria, Aspergillus or the saccharomycete activated, inoculum concentration 0.21% Volume, fermentation temperature are 28.4 DEG C, and fermentation pH is 4.5, fermentation time 21h, and 83 DEG C of sterilizing 18min, are filtered after the completion of fermentation, filter Liquid 5800r/min centrifuges 15.9min, and solvent is recovered under reduced pressure in 47 DEG C of supernatant, and proportion is 1.13 medicinal extract when being condensed into 40 DEG C, cold Lyophilized dry natural component fermentate；Described grape-kernel oil is made by milling process.

4. the nanoemulsion essence according to claim 1 containing natural component extract, it is characterised in that by following heavy The raw material of amount percentages is made：Soybean lecithin 1.68%, Arabic gum 2.56%, cithrol 2.53%, west Bassora gum 0.64%, peach gum 0.46%, polyoxyethylene (20) sorbitan mono-oleic acid ester 3.82%, citric acid 0.71%, second two Alcohol 3.77%, glycerine 4.61%, vitamin E 0.11%, grape-kernel oil 3.89%, olive oil 2.58%, soybean oil 3.31%, in Medicine compound fermentation thing 1.13%, Chinese medicine compound prescription fermentation polysaccharides 1.67%, natural component fermentate 1.73%, deionized water 64.8%；

Wherein, the preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, Cape jasmine 1.5 parts of son, 1.2 parts of the bletilla striata, 1 part of kuh-seng, it is well mixed, is ground into coarse powder, is placed in flash extracter and extracts 3 times, adds every time Enter 27.6 times of 45.6% ethanol (V/V) measured of the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, Sophora flavescens gross weight, each extraction time For 4.8min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportion when being concentrated under reduced pressure into 40 DEG C is 1.23,5500r/ Min centrifuges 15min, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, Inoculum concentration is 22.8% volume, and fermentation temperature is 46.1 DEG C, and fermentation pH is 6.7, fermentation time 46h, and 121 DEG C go out after the completion of fermentation Bacterium 61min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter Slag a adds the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized water of 17.6 times of amounts of Sophora flavescens gross weight, 98.5 DEG C of refluxing extractions 115min, filtering, proportion is 1.21 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifuges 16min, and supernatant adds Entering ethanol makes alcohol content remove supernatant, lower sediment up to 81.2% (V/V), 4 DEG C of standing 24h, 5700r/min centrifugation 15min Washed with the absolute ethyl alcohol of 11 times of weight of precipitation, 5300r/min centrifugations 10min removes supernatant, and lower sediment sevage methods take off Albumen, it is freeze-dried to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take in parts by weight 2.5 parts of Flos Magnoliae Officinalis, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, pseudo-ginseng 2.2 parts are spent, is well mixed, is ground into fine powder, is placed in flash extracter and extracts 2 times, add Flos Magnoliae Officinalis, flower of Panax ginseng, Pueraria lobota every time Root flower, Astragalis flower, okra, rose, 18.7 times of 47% ethanol (V/V) measured of sanchi flower total weight, when extracting every time Between be 2.7min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.17,5700r/min centrifugations 18min, the one or more of arbitrary proportions mixing for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated, Inoculum concentration is 18.9% volume, and fermentation temperature is 45.7 DEG C, and fermentation pH is 6.9, fermentation time 38h, and 122 DEG C go out after the completion of fermentation Solvent is recovered under reduced pressure in bacterium 70min, filtering, filtrate 5800r/min centrifugation 15.9min, 47 DEG C of supernatant, proportion when being condensed into 40 DEG C For 1.19 medicinal extract, natural component fermentate is freeze-dried to obtain；Described grape-kernel oil is made by milling process.

5. the nanoemulsion essence according to claim 1 containing natural component extract, it is characterised in that by following heavy The raw material of amount percentages is made：Soybean lecithin 1.45%, Arabic gum 1.75%, cithrol 1.42%, west Bassora gum 0.50%, peach gum 0.35%, polyoxyethylene (20) sorbitan mono-oleic acid ester 1.45%, citric acid 0.42%, second two Alcohol 2.75%, glycerine 3.57%, vitamin E 0.08%, grape-kernel oil 2.76%, olive oil 1.81%, soybean oil 2.24%, in Medicine compound fermentation thing 1.05%, Chinese medicine compound prescription fermentation polysaccharides 1.56%, natural component fermentate 1.55%, deionized water 75.29%；Wherein, the preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, ginseng 1.7 Part, 1.5 parts of cape jasmine, 1.2 parts of the bletilla striata, 1 part of kuh-seng, it is well mixed, is ground into coarse powder, is placed in flash extracter and extracts 3 times, often Secondary 18.7 times of 45.6% ethanol (V/V) measured for adding the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, Sophora flavescens gross weight, every time extraction Time is 3.5min, and filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.17, 5500r/min centrifuges 15min, takes supernatant to access the arbitrary proportion of the lactobacillus activated or one or both of saccharomycete Mixing, inoculum concentration are 0.56%-22.8% volumes, and fermentation temperature is 36.6 DEG C, and fermentation pH is 5.5, fermentation time 36h, is fermented After the completion of 102 DEG C of sterilizing 39min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant is dried under reduced pressure, and obtains Chinese medicine Compound fermentation thing；The filter residue a additions bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized waters of 13.2 times of amounts of Sophora flavescens gross weight, 92.2 DEG C refluxing extraction 88min, filtering, proportion is 1.17 medicinal extract when filtrate decompression is concentrated into 40 DEG C, 5400r/min centrifugation 16min, Supernatant, which adds ethanol, makes alcohol content remove supernatant up to 81.2% (V/V), 4 DEG C of standing 16h, 5700r/min centrifugation 15min, Lower sediment is washed with the absolute ethyl alcohol of 11 times of weight of precipitation, and 5300r/min centrifugations 10min removes supernatant, lower sediment Sevage methods take off albumen, are freeze-dried to obtain Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take 2.5 parts of Flos Magnoliae Officinalis in parts by weight, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, rose 2.3 parts, 2.2 parts of sanchi flower, be well mixed, be ground into fine powder, be placed in flash extracter and extract 2 times, every time addition Flos Magnoliae Officinalis, Flower of Panax ginseng, flower of Radix Puerariae, Astragalis flower, okra, rose, 12.7 times of 47% ethanol (V/V) measured of sanchi flower total weight, often Secondary extraction time is 2.0min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.11,5700r/ Min centrifuges 18min, the one or more of any ratios for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated Example mixing, inoculum concentration are 9.1% volume, and fermentation temperature is 36.8 DEG C, and fermentation pH is 5.7, fermentation time 29.5h, and fermentation is completed 103 DEG C of sterilizing 44min afterwards, filtering, filtrate 5800r/min centrifugation 15.9min, 47 DEG C of supernatant are recovered under reduced pressure solvent, are condensed into Proportion is 1.16 medicinal extract at 40 DEG C, is freeze-dried to obtain natural component fermentate；Described grape-kernel oil is made by milling process.

6. the nanoemulsion essence according to claim 1 containing natural component extract, it is characterised in that by following heavy The raw material of amount percentages is made：Soybean lecithin 1.14%, Arabic gum 2.56%, cithrol 1.42%, west Bassora gum 0.64%, peach gum 0.35%, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73%, citric acid 0.12%, second two Alcohol 1.89%, glycerine 4.61%, vitamin E 0.11%, grape-kernel oil 2.76%, olive oil 1.81%, soybean oil 3.31%, in Medicine compound fermentation thing 0.96%, Chinese medicine compound prescription fermentation polysaccharides 1.67%, natural component fermentate 1.55%, deionized water 74.37%；

Wherein, the preparation method of described Chinese medicine compound prescription fermentate is：Take 2 parts of the bark of official magnolia in parts by weight, 1.7 parts of ginseng, Cape jasmine 1.5 parts of son, 1.2 parts of the bletilla striata, 1 part of kuh-seng, it is well mixed, is ground into coarse powder, is placed in flash extracter and extracts 3 times, adds every time Enter 45.6% ethanol (V/V) of the bark of official magnolia, ginseng, cape jasmine, the bletilla striata, 9.3 times of Sophora flavescens gross weight amounts, each extraction time is 3.5min, filtering, filter residue a is standby, merging filtrate, the medicinal extract that 45 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.23,5500r/min 15min is centrifuged, takes the arbitrary proportion mixing of lactobacillus or one or both of saccharomycete that supernatant access has activated, inoculation It is 27.8 DEG C to measure as 22.8% volume, fermentation temperature, and fermentation pH is 4.4, fermentation time 46h, 102 DEG C of sterilizings after the completion of fermentation 17min, filtering, filtrate 5600r/min centrifugation 15.5min, 50 DEG C of supernatant are dried under reduced pressure, and obtain Chinese medicine compound prescription fermentate；Filter residue a The addition bark of official magnolia, ginseng, cape jasmine, the bletilla striata, the deionized waters of 8.5 times of amounts of Sophora flavescens gross weight, 92.2 DEG C of refluxing extraction 88min, Filtering, proportion is 1.13 medicinal extract when filtrate decompression is concentrated into 40 DEG C, and 5400r/min centrifugation 16min, supernatant, which adds ethanol, to be made Alcohol content removes supernatant, lower sediment precipitation 11 up to 81.2% (V/V), 4 DEG C of standing 24h, 5700r/min centrifugation 15min The absolute ethyl alcohol washing of times weight, 5300r/min centrifugations 10min remove supernatant, and lower sediment sevage methods take off albumen, freezing Dry Chinese medicine compound prescription fermentation polysaccharides；Described natural component fermentate its preparation method is：Take Flos Magnoliae Officinalis in parts by weight 2.5 parts, 2 parts of flower of Panax ginseng, 1 part of flower of Radix Puerariae, 1.5 parts of Astragalis flower, 1.6 parts of okra, 2.3 parts of rose, 2.2 parts of sanchi flower, It is well mixed, fine powder is ground into, is placed in flash extracter and extracts 2 times, add Flos Magnoliae Officinalis, flower of Panax ginseng, flower of Radix Puerariae, the Radix Astragali every time Flower, okra, rose, 47% ethanol (V/V) of 18.7 times of sanchi flower total weight amounts, each extraction time be 1.3min, filtering, merging filtrate, the medicinal extract that 43 DEG C of proportions when being concentrated under reduced pressure into 40 DEG C are 1.11,5700r/min centrifugation 18min, The one or more of arbitrary proportions mixing for taking supernatant to access in lactic acid bacteria, Aspergillus or the saccharomycete activated, inoculum concentration For 18.9% volume, fermentation temperature is 28.4 DEG C, and fermentation pH is 5.7, fermentation time 29.5h, 83 DEG C of sterilizings after the completion of fermentation Solvent is recovered under reduced pressure in 18min, filtering, filtrate 5800r/min centrifugation 15.9min, 47 DEG C of supernatant, and proportion is when being condensed into 40 DEG C 1.19 medicinal extract, it is freeze-dried to obtain natural component fermentate；Described grape-kernel oil is made by milling process.

7. the preparation method of the nanoemulsion essence according to claim 2 containing natural component extract, its feature exist In comprising the following steps：

(1) citric acid 0.12kg, Arabic gum 0.83kg, tragacanth 0.27kg, peach gum 0.24kg are taken, is well mixed, is added Deionized water 9.93kg dissolves, then sequentially add the soybean lecithin 1.14kg of recipe quantity, cithrol 0.34kg, Polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73kg, be well mixed, sequentially add recipe quantity grape-kernel oil 0.66kg, Olive oil 1.05kg, soybean oil 1.12kg, vitamin E 0.06kg, ethylene glycol 1.89kg, glycerine 2.46kg, it is well mixed, must receives Rice milk Essence matrix；

(2) Chinese medicine compound prescription fermentate 0.96kg, Chinese medicine compound prescription fermentation polysaccharides 1.45kg, natural component fermentate 1.35kg are taken, is mixed Close uniformly, adding 62 DEG C of deionized water 22.56kg makes dissolving, puts to room temperature, obtains natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 41 DEG C, then in the situation of stirring Under be slowly added to 47 DEG C of deionized water 52.84kg, be well mixed, put to room temperature, that is, obtain described carrying containing natural component Take the nanoemulsion essence of thing.

8. the preparation method of the nanoemulsion essence according to claim 2 containing natural component extract, its feature exist In comprising the following steps：

(1) citric acid 0.71kg, Arabic gum 2.56kg, tragacanth 0.64kg, peach gum 0.46kg are taken, is well mixed, is added Deionized water 32.78kg dissolves, and sequentially adds the soybean lecithin 1.68kg of recipe quantity, cithrol 2.53kg, poly- Oxygen ethene (20) sorbitan mono-oleic acid ester 3.82kg, it is well mixed, sequentially adds grape-kernel oil 3.89kg, the olive of recipe quantity Oily 2.58kg, soybean oil 3.31kg, vitamin E 0.11kg, ethylene glycol 3.77kg, glycerine 4.61kg, it is well mixed, obtains nano-emulsion Essence matrix；

(2) Chinese medicine compound prescription fermentate 1.13kg, Chinese medicine compound prescription fermentation polysaccharides 1.67kg, natural component fermentate 1.73kg are taken, is mixed Close uniformly, adding 62 DEG C of deionized water 39.83kg makes dissolving, puts to room temperature, obtains natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 52 DEG C, then in the situation of stirring Under be slowly added to 47 DEG C of deionized water 1.54kg, be well mixed, put to room temperature, that is, obtain described extracting containing natural component The nanoemulsion essence of thing.

9. the preparation method of the nanoemulsion essence according to claim 2 containing natural component extract, its feature exist In comprising the following steps：

(1) citric acid 0.42kg, Arabic gum 1.75kg, tragacanth 0.50kg, peach gum 0.35kg are taken, is well mixed, is added Deionized water 21.59kg makes dissolving, sequentially adds soybean lecithin 1.45kg, cithrol 1.42kg, polyoxyethylene (20) sorbitan mono-oleic acid ester 1.45kg, it is well mixed, sequentially adds grape-kernel oil 2.76kg, olive oil 1.81kg, soybean Oily 2.24kg, vitamin E 0.08, ethylene glycol 2.75kg, glycerine 3.57kg, it is well mixed, obtains nanoemulsion essence matrix；

(2) Chinese medicine compound prescription fermentate 1.05kg, Chinese medicine compound prescription fermentation polysaccharides 1.56kg, natural component fermentate 1.55kg are taken, is mixed Close uniformly, adding 62 DEG C of deionized water 25.38kg makes dissolving, and solution is put to room temperature, obtains natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 46 DEG C, then in the situation of stirring Under be slowly added to 47 DEG C of deionized water 28.23kg, be well mixed, put to room temperature, that is, obtain described carrying containing natural component Take the nanoemulsion essence of thing.

10. the preparation method of the nanoemulsion essence according to claim 2 containing natural component extract, its feature exist In comprising the following steps：

(1) citric acid 0.12kg, Arabic gum 2.56kg, tragacanth 0.64kg, peach gum 0.35kg are taken, is well mixed, is added Deionized water 24.96kg makes dissolving, sequentially adds soybean lecithin 1.14kg, cithrol 1.42kg, polyoxyethylene (20) sorbitan mono-oleic acid ester 0.73kg, it is well mixed, sequentially adds grape-kernel oil 2.76kg, the olive oil of recipe quantity 1.81kg, soybean oil 3.31kg, vitamin E 0.11kg, ethylene glycol 1.89kg, glycerine 4.61kg, it is well mixed, obtains a nanometer milk extract Magnificent liquid-based matter；

(2) Chinese medicine compound prescription fermentate 0.96kg, Chinese medicine compound prescription fermentation polysaccharides 1.67kg, natural component fermentate 1.55kg are taken, is mixed Close uniformly, adding 62 DEG C of deionized water 25.50kg makes dissolving, puts to room temperature, obtains natural component extract solution；

(3) nanoemulsion essence matrix is well mixed with natural component extract solution at 41 DEG C, then in the situation of stirring Under be slowly added to 47 DEG C of deionized water 23.91kg, be well mixed, put to room temperature, that is, obtain described carrying containing natural component Take the nanoemulsion essence of thing.