CN107446845A - Composite bacteria fermentation aquatic products discarded object prepares the method and purposes of polypeptide mixed fish soluble meal - Google Patents
Composite bacteria fermentation aquatic products discarded object prepares the method and purposes of polypeptide mixed fish soluble meal Download PDFInfo
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Abstract
The invention discloses the method and purposes that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, preparation method includes preparation, solid state fermentation, the drying of solid-state fermentation culture medium.Composite bacteria agent is the Neuraspora crassa that preserving number is CGMCC No.13955 Lactobacillus plantarum, preserving number is CGMCC No.13953 bacillus subtilis, preserving number is CGMCC No.13769 and the bacillus coagulans that preserving number is CGMCC No.13954.Have the beneficial effect that：Using marine organisms processing waste or cheap aquatic product as fermentation raw material, both support can be provided for the feasibility of its utilization, and increase its added value, save the preparation cost of polypeptide mixed fish soluble meal again；The polypeptide mixed fish soluble meal high protein low fat being prepared, small peptide content is high and is rich in carotenoid, organic acid, oligosaccharide and beneficial bacterium isoreactivity material, and alternative fish meal is used for safe feed industry, feed additive industry.
Description
Technical field
The present invention relates to processing of aquatic products waste's reclamation, turning waste into wealth prepares animal feed protein raw materials field,
Specifically a kind of composite bacteria fermentation aquatic products discarded object prepares the method and purposes of polypeptide mixed fish soluble meal.
Background technology
In the middle and later periods seventies in last century, China's feed industry makes first appearance, and merely through more than 10 years pioneer an enterprise with painstaking efforts, just walks
The development course of developed country's many decades in the world has been crossed, from 1992, the second in the world is sure to occupy in continuous 17 years with forage yield
Position turns into feed industry big country.Under the drive of new scientific and technological revolution, by the development of 30 years, China's feed industry was to common people's exhibition
Great potential is shown, by 2011, feed industry total output leapt to the first in the world up to 1.81 hundred million tons.With China's feedstuff industry
Fast development, enterprise goes from strength to strength, and forage yield remains very fast growth trend, therefore is also in the demand of feedstuff
Rigidity increases.More than 200 ten thousand tons of fish meal of the annual feed in China at present, but domestic fish meal is considerably less, ten thousand tons only more than 40, to fish meal
The interdependency nearly more than 80% of import；In terms of the bulk feed raw material supply such as dregs of beans, rice bran, corn, based on cultivated area and list
The factors such as production technology, growth space is also extremely limited, and the supply of whole feedstuff is in tight poised state, feed resource
Imbalance between supply and demand increasingly sharpens, and is shown according to recent statistics Monitoring Data, China's forage yield in 2013 to 2015 over 30 years first
Decline.Feed industry is the important component of agricultural, under aquaculture and the background of feed industry sustained and rapid development,
How corn is broken through -- bean pulp type feed formula technology, new feedstuff and safe and reliable substitute are developed, accomplish to hold
Supervention exhibition feed industry turns into feedstuff industry urgent problem to be solved.How from specific natural conditions, by science skill
Art, correctly handle well resource and environment, using with protection, currently with long-range, the survival and development relations, walk out one and rationally match somebody with somebody
Put and utilize resource, improve resource utilization, realize resource continuous utilization, produce high yield, high-quality, low consumption, the height of sustainable development
Effect development is even more the problem of being currently needed for thinking deeply and study.
Fish meal is used as current optimal animal protein source, and it has biological value height, energy valency in raising is applied
It is worth a variety of advantages such as height, calcic phosphorus height, micronutrient levels height, digestibility height, is animal（Poultry, fowl, fish）Aquaculture it is advanced
Feed.It is especially particularly important to production aquatic feeds, particularly shrimp feed, Terrapin feed, marine fish feed and piglet feed.
Although in the market has the product that can much substitute fish meal, such as the animal/vegetable protein such as meat meal tankage, blood meal, dregs of beans, rapeseed meal,
But still without any status that can shake the optimal animal protein source of fish meal.Compared with fish meal, various replacement fish meal
The problems such as ANFs, palatability, amino acid imbalance and digestibility to varying degrees be present in protein raw materials.
With the fast development of intensive culture, the demand of fish meal steeply rises.China is fish meal maximum in the world
Country of consumption, fish meal annual requirement is in the trend risen year by year up to more than 200 ten thousand tons, and wherein more than 80% still relies on import.But
It is that influence phenomena such as due to overfishing and EI Nino, the total output of world's fish meal just declines year by year, and fish meal prices are all the way
Raise up, more because fish meal is as a kind of scarce resource, in each main state of origin by strict limited production and protection, so as to serious
Limit the further development of aquaculture.
About 100,000,000 tons of the annual production of current global aquatic products, wherein processing of aquatic products discarded object and cheap aquatic product account for 40%
More than, it is dropped mostly or as feed, fertilizer.Output of aquatic products in China accounts for 1/3 or so of world's aquatic products total output,
Occupy the first in the world.The East Sea and Huanghai Sea and Bohai Sea domain it is annual Hairtail catch it is very big, the catch of the annual hairtail in Near East sea just accounts for
The 40-45% of total amount.Substantial amounts of eupleurogrammus muticus and Optimization of Low Value Fish can only be used for producing feed, and utilization rate is low, and economic value is not high, and
Serious environmental pollution can also be caused.And in recent years due to water pollution and excessive amount of fishing, it is the low value such as small fish and shrimp in catches, low
The ratio of matter aquatic products improves constantly.Because coastal waters and inland waters economic fish resource are increasingly reduced, cause small fish and shrimp etc. low
The amount of fishing of value aquatic products greatly increases；Further, since the technology content of processing of aquatic products is relatively low, caused leftover bits and pieces and other
The amount of pair material is very big.During processing of aquatic products, substantial amounts of leftover bits and pieces can be produced, in fish head, fish-skin, fin, fish tail, fish
Dirty, fish-bone, fish courage, float for fishing, shrimp shell, crab shell, shellfish and its residual flesh of fish, its weight account for the 40%~55% of raw material fish.Aquatic products
Lived in product leftover bits and pieces containing abundant nutriment and useful component, the even also certain functional characteristic of some components and physiology
Property, thus be a kind of important living resources.This kind of leftover bits and pieces largely goes out of use, and not only pollutes environment, also creates resource
Waste.
The content of the invention
It is an object of the invention to provide a kind of method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal
With purposes, the polypeptide mixed fish soluble meal being prepared, its high protein low fat, small peptide content is high and rich in carotenoid, organic acid, low
Glycan and beneficial bacterium isoreactivity material, alternative fish meal are used for safe feed industry.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Composite bacteria fermentation aquatic products give up
The method that gurry prepares polypeptide mixed fish soluble meal, composite bacteria agent include Lactobacillus plantarum, bacillus subtilis, Neuraspora crassa and condensation
Bacillus.
The preserving number of Lactobacillus plantarum is CGMCC No.13955, and Classification And Nomenclature is Lactobacillus plantarum（Lactobacillus plantarum）, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on March 28th, 2017
（CGMCC）；The preserving number of bacillus subtilis is CGMCC No.13953, and Classification And Nomenclature is bacillus subtilis（Bacillus subtilis）, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on March 28th, 2017
（CGMCC）；The preserving number of Neuraspora crassa is CGMCC No.13769, and Classification And Nomenclature is Neuraspora crassa（Neurospora crassa）, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on March 28th, 2017
（CGMCC）；The preserving number of bacillus coagulans is CGMCC No.13954, and Classification And Nomenclature is bacillus coagulans（Bacillus coagulans）, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on March 28th, 2017
（CGMCC）.China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）Positioned at the Chaoyang District, Beijing City North Star
The institute 3 of West Road 1.Above-mentioned strain, which cooperates, produces unexpected effect, and composite bacteria agent is from marine organisms processing waste
Or carbon source, nitrogen source and various growth factors are obtained in cheap aquatic product, growth and breeding is vigorous, and bacterial activity is high；Marine organisms add
Work discarded object or cheap aquatic product are decomposed by composite bacteria fermentation, and high molecular weight protein is decomposed into small molecular protein or polypeptide even
Amino acid；Destroy aquatic products zooblast, cell liquid outflow；Composite bacteria agent metabolism produces novel substance.What is finally obtained is more
Peptide mixed fish soluble meal, its high protein low fat, small peptide content is high and is rich in carotenoid, organic acid, oligosaccharide and beneficial bacterium isoreactivity thing
Matter, alternative fish meal are used for safe feed industry.
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, include the system of solid-state fermentation culture medium
Standby, solid state fermentation, drying.The polypeptide mixed fish soluble meal high protein low fat that fermentation obtains, small peptide content is high to be simultaneously rich in carotenoid, has
Machine acid, oligosaccharide and beneficial bacterium isoreactivity material, alternative fish meal are used for safe feed industry.Livestock and poultry cultivation is used it for, can
The protein and vitamin content of birds, beasts and eggs are improved, reduces cholesterol level, improves the quality of birds, beasts and eggs；Use it for aquatic livestock class
Cultivation, it is possible to provide the nutriment needed for the growth of aquiculture animal class, improve beneficial bacteria of intestinal tract flora, promote aquaculture to move
Species are digested and assimilated to feed, are reduced excreta, are played growth promoting function.
The preparation process of solid-state fermentation culture medium includes the thing of marine organisms processing waste or cheap aquatic product to choose
Matter is fermentation raw material, is mixed after cleaned, draining and is homogenized and sterilizes, obtains solid-state fermentation culture medium.It is useless with marine organisms processing
Gurry or cheap aquatic product are fermentation raw material, both can provide support for the feasibility of its utilization, and increase its added value, again
Save the preparation cost of polypeptide mixed fish soluble meal.
Solid state fermentation step is that composite bacteria agent and water are added in solid-state fermentation culture medium, and inoculum concentration is 4 ~ 10wt%, plant
Lactobacillus：Bacillus subtilis：Neuraspora crassa：Bacillus coagulans mass ratio is 0.6 ~ 1.5:1.2~2.8: 0.6~1.5：
0.6~1.5；Material-water ratio is 1:0.6 ~ 1, fermentation time is 35 ~ 60h.Composite bacteria agent both fully ferments marine organisms under above-mentioned condition
Processing waste or cheap aquatic product, microbial inoculum and waste of time will not be caused again.
Solid state fermentation uses low temperature drying processing technology, and early stage, material temperature was less than 45 DEG C, and later stage material temperature is less than 60 DEG C.
After tunning drying is less than 10% to water content, it crushed 40 ~ 60 mesh sieves and obtain polypeptide mixed fish soluble meal.
Composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, for replacement fish meal cultured fishes, the use of livestock and poultry
On the way.
Compared with prior art, the advantage of the invention is that：
Both can be the feasibility of its utilization 1. using marine organisms processing waste or cheap aquatic product as fermentation raw material
Support is provided, increases its added value, saves the preparation cost of polypeptide mixed fish soluble meal again.
2. strain, which cooperates, produces unexpected effect, composite bacteria agent is from marine organisms processing waste or low value
Carbon source, nitrogen source and various growth factors are obtained in aquatic products, growth and breeding is vigorous, and bacterial activity is high；Marine organisms processing is discarded
Thing or cheap aquatic product are decomposed by composite bacteria fermentation, and high molecular weight protein is decomposed into small molecular protein or polypeptide even amino
Acid；Destroy aquatic products zooblast, cell liquid outflow；Composite bacteria agent metabolism produces novel substance.The polypeptide fish finally obtained
Fine powder, its high protein low fat, small peptide content is high and is rich in carotenoid, organic acid, oligosaccharide and beneficial bacterium isoreactivity material,
Alternative fish meal is used for safe feed industry.
3. the inventive method is simple to operate, each step has accurate data, favorable reproducibility, it is not necessary to wastewater treatment link,
It is less to pollute environment, post processing is easy to process, is adapted to large-scale production.
4. polypeptide mixed fish soluble meal prepared by the present invention is used for livestock and poultry cultivation, the protein and vitamin that can improve birds, beasts and eggs contain
Amount, cholesterol level is reduced, improve the quality of birds, beasts and eggs；Use it for aquaculture, it is possible to provide aquiculture animal class grows institute
The nutriment needed, improve beneficial bacteria of intestinal tract flora, promote aquiculture animal class to digest and assimilate feed, reduce excretion
Thing, play growth promoting function.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, composite bacteria agent include Lactobacillus plantarum, withered grass
Bacillus, Neuraspora crassa and bacillus coagulans.
The preserving number of Lactobacillus plantarum is CGMCC No.13955；The preserving number of bacillus subtilis is CGMCC
No.13953；The preserving number of Neuraspora crassa is CGMCC No.13769；The preserving number of bacillus coagulans is CGMCC
No.13954.Above-mentioned strain, which cooperates, produces unexpected effect, and composite bacteria agent is from marine organisms processing waste or low
It is worth in aquatic products and obtains carbon source, nitrogen source and various growth factors, growth and breeding is vigorous, and bacterial activity is high；Marine organisms processing is useless
Gurry or cheap aquatic product are decomposed by composite bacteria fermentation, and high molecular weight protein is decomposed into small molecular protein or polypeptide even amino
Acid；Destroy aquatic products zooblast, cell liquid outflow；Composite bacteria agent metabolism produces novel substance.The polypeptide fish finally obtained
Fine powder, its high protein low fat, small peptide content is high and is rich in carotenoid, organic acid, oligosaccharide and beneficial bacterium isoreactivity material,
Alternative fish meal is used for safe feed industry.
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, include the system of solid-state fermentation culture medium
Standby, solid state fermentation, drying.The polypeptide mixed fish soluble meal high protein low fat that fermentation obtains, small peptide content is high to be simultaneously rich in carotenoid, has
Machine acid, oligosaccharide and beneficial bacterium isoreactivity material, alternative fish meal are used for safe feed industry.Livestock and poultry cultivation is used it for, can
The protein and vitamin content of birds, beasts and eggs are improved, reduces cholesterol level, improves the quality of birds, beasts and eggs；Use it for aquaculture,
Nutriment needed for the growth of aquiculture animal class can be provided, improve beneficial bacteria of intestinal tract flora, promote aquiculture animal class
Feed is digested and assimilated, excreta is reduced, plays growth promoting function.
The preparation process of solid-state fermentation culture medium includes the thing of marine organisms processing waste or cheap aquatic product to choose
Matter is fermentation raw material, is mixed after cleaned, draining and is homogenized and sterilizes, obtains solid-state fermentation culture medium.It is useless with marine organisms processing
Gurry or cheap aquatic product are fermentation raw material, both can provide support for the feasibility of its utilization, and increase its added value, again
Save the preparation cost of polypeptide mixed fish soluble meal.
Solid state fermentation step is that composite bacteria agent and water are added in solid-state fermentation culture medium, inoculum concentration 8wt%, plant breast
Bacillus：Bacillus subtilis：Neuraspora crassa：Bacillus coagulans mass ratio is 1:2: 1：1；Material-water ratio is 1:0.8, fermentation
Time is 38 ~ 40h.Composite bacteria agent both fully ferments marine organisms processing waste or cheap aquatic product under above-mentioned condition, and not
Microbial inoculum and waste of time can be caused.
Solid state fermentation uses low temperature drying processing technology, early stage 42 ~ 45 DEG C of material temperature, 55 ~ 60 DEG C of later stage material temperature.
After tunning drying is less than 10% to water content, it crushed 60 ~ 80 mesh sieves and obtain polypeptide mixed fish soluble meal.
Composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, for replacement fish meal cultured fishes, the use of livestock and poultry
On the way.
Embodiment 2：
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, composite bacteria agent include Lactobacillus plantarum, withered grass
Bacillus, Neuraspora crassa and bacillus coagulans.
The preserving number of Lactobacillus plantarum is CGMCC No.13955；The preserving number of bacillus subtilis is CGMCC
No.13953；The preserving number of Neuraspora crassa is CGMCC No.13769；The preserving number of bacillus coagulans is CGMCC
No.13954。
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, comprises the following steps：
1）The preparation of solid-state fermentation culture medium：It is fermentation raw material to choose trash fish, leftovers of tilapia, the hairtail spines such as eupleurogrammus muticus,
Weight ratio is 0.9:1:1.25, after cleaned, draining mixing homogenate be made fish homogenate, then by fish homogenate and yam skin, bean dregs, rice
Chaff is well mixed, and the 35min that sterilized in 121 DEG C obtains solid-state fermentation culture medium；
2）Solid state fermentation step is that composite bacteria agent and water, inoculum concentration 7wt%, plant breast bar are added in solid-state fermentation culture medium
Bacterium：Bacillus subtilis：Neuraspora crassa：Bacillus coagulans mass ratio is 0.8:2.1: 0.8：0.8；Material-water ratio is 1:
0.7, fermentation time is 56 ~ 60h.Solid state fermentation uses low temperature drying processing technology, early stage 38 ~ 40 DEG C of material temperature, later stage material temperature 48 ~
50℃；
3）After tunning drying is less than 10% to water content, it crushed 60 ~ 80 mesh sieves and obtain polypeptide mixed fish soluble meal.
Composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, for replacing fish culture feed fish meal cultivation fish
Class,
Embodiment 3：
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, composite bacteria agent include Lactobacillus plantarum, withered grass
Bacillus, Neuraspora crassa and bacillus coagulans.
The preserving number of Lactobacillus plantarum is CGMCC No.13955；The preserving number of bacillus subtilis is CGMCC
No.13953；The preserving number of Neuraspora crassa is CGMCC No.13769；The preserving number of bacillus coagulans is CGMCC
No.13954。
The method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, comprises the following steps：
1）The preparation of solid-state fermentation culture medium：Choose the discarded object after cod, salmon, tuna processing, shrimp shell（Head）With it is low
Value aquatic products are fermentation raw material, and weight ratio is 0.9:1.1:1.5:0.7：1.6, after cleaned, draining mixing homogenate it is even that fish is made
Slurry, then fish is homogenized and is well mixed with dregs of beans, soybean residue, rice bran, the 35min that sterilized in 121 DEG C obtains solid-state fermentation culture medium；
2）Solid state fermentation step is that composite bacteria agent and water, inoculum concentration 8wt%, plant breast bar are added in solid-state fermentation culture medium
Bacterium：Bacillus subtilis：Neuraspora crassa：Bacillus coagulans mass ratio is 0.8:2: 0.8：0.8；Material-water ratio is 1:0.8,
Fermentation time is 56 ~ 60h.Solid state fermentation uses low temperature drying processing technology, early stage 38 ~ 40 DEG C of material temperature, later stage material temperature 48 ~ 50
℃；
3）After tunning drying is less than 10% to water content, it crushed 60 ~ 80 mesh sieves and obtain polypeptide mixed fish soluble meal.
Composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, for replacing fish meal healthy aquaculture fish, livestock and poultry
The purposes of healthy aquaculture.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Claims (10)
1. the method that composite bacteria fermentation aquatic products discarded object prepares polypeptide mixed fish soluble meal, it is characterised in that：Described composite bacteria agent
Including Lactobacillus plantarum, bacillus subtilis, Neuraspora crassa and bacillus coagulans.
2. the method that composite bacteria fermentation aquatic products discarded object according to claim 1 prepares polypeptide mixed fish soluble meal, its feature
It is：The preserving number of described Neuraspora crassa is CGMCC No.13769.
3. the method that composite bacteria fermentation aquatic products discarded object according to claim 1 prepares polypeptide mixed fish soluble meal, its feature
It is：The preserving number of described bacillus subtilis is CGMCC No.13953.
4. the method that composite bacteria fermentation aquatic products discarded object according to claim 1 prepares polypeptide mixed fish soluble meal, its feature
It is：The preserving number of described bacillus coagulans is CGMCC No.13954.
5. the method that composite bacteria fermentation aquatic products discarded object according to claim 1 prepares polypeptide mixed fish soluble meal, its feature
It is：The preserving number of described Lactobacillus plantarum is CGMCC No.13955.
6. the method that composite bacteria fermentation aquatic products discarded object according to claim 1 prepares polypeptide mixed fish soluble meal, its feature
It is：Described method includes preparation, solid state fermentation and the drying of solid-state fermentation culture medium.
7. the method that composite bacteria fermentation aquatic products discarded object according to claim 6 prepares polypeptide mixed fish soluble meal, its feature
It is：The preparation process of described solid-state fermentation culture medium is to choose to include marine organisms processing waste or cheap aquatic product
Material is fermentation raw material, is mixed after cleaned, draining and is homogenized and sterilizes, obtains solid-state fermentation culture medium.
8. the method that composite bacteria fermentation aquatic products discarded object according to claim 6 prepares polypeptide mixed fish soluble meal, its feature
It is：Described solid state fermentation step is the composite bacteria agent and water after addition activation, inoculum concentration 4 in solid-state fermentation culture medium
~ 10wt%, Lactobacillus plantarum：Bacillus subtilis：Neuraspora crassa：Bacillus coagulans mass ratio is 0.6 ~ 1.5:1.2~
2.8: 0.6~1.5：0.6~1.5；Material-water ratio is 1:0.6 ~ 1, fermentation time is 35 ~ 60h.
9. the method that composite bacteria fermentation aquatic products discarded object according to claim 6 prepares polypeptide mixed fish soluble meal, its feature
It is：Described solid state fermentation uses low temperature drying processing technology, and early stage, material temperature was less than 45 DEG C, and later stage material temperature is less than 60 DEG C.
10. composite bacteria fermentation aquatic products discarded object prepares the purposes of polypeptide mixed fish soluble meal, it is characterised in that is used to support instead of fish meal
Breeding fish, the purposes of livestock and poultry.
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| CN101564081A (en) * | 2008-04-25 | 2009-10-28 | 上海创博生态工程有限公司 | Production method for producing fish meal replacement products by utilizing fermentation process |
| CN103725738A (en) * | 2013-12-17 | 2014-04-16 | 浙江工商大学 | Method for preparing collagen polypeptides by using larimichthys crocea leftovers |
| CN106343151A (en) * | 2016-08-18 | 2017-01-25 | 深圳市中雁生物工程有限公司 | Production method of animal feed enriched in carotenoid and probiotics |
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