CN107446801A - It is a kind of to rotate and with the micro algae culturing device of light reflection - Google Patents

It is a kind of to rotate and with the micro algae culturing device of light reflection Download PDF

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Publication number
CN107446801A
CN107446801A CN201710821733.5A CN201710821733A CN107446801A CN 107446801 A CN107446801 A CN 107446801A CN 201710821733 A CN201710821733 A CN 201710821733A CN 107446801 A CN107446801 A CN 107446801A
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Prior art keywords
rotating
algae culturing
micro algae
light
light source
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CN201710821733.5A
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CN107446801B (en
Inventor
游奎
钟喆
陈泽文
马彩华
张芳
朱玲
马洪钢
刘心田
徐华
王绍军
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Nanjing Dream Biotechnology Co Ltd
Dalian University of Technology
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Nanjing Dream Biotechnology Co Ltd
Dalian University of Technology
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/08Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a kind of rotate and with the micro algae culturing device of light reflection, it is the spherical bilayer housing with rotation on pedestal, its upper end is ventilative and peep hole, spherical bilayer housing outer layer is made up of the opaque material with enough structural strengths, and internal layer is mirror surface structure of the liner on outer casing.Sphere center position is light source and protective cover, is connected with pedestal to supply electric power, the not pivotable parts of constituent apparatus, transparency during microalgae concentration peak is less than with the distance of liner minute surface.Pedestal rotating part sets more than two charging stirring jet pipes, is rotated with housing, the pivotable parts of constituent apparatus.The centrifugal inertial force that rotating part of the present invention is provided may act as the effect of gravity, therefore can carry out the culture of microalgae under agravic and microgravity condition in the outer space and aerospace environment.The present invention also is adapted for carrying out the culture of microalgae under gravity condition, has the characteristics that optical energy utilization efficiency height, microdisk electrode ambient stable, application prospect is good.

Description

It is a kind of to rotate and with the micro algae culturing device of light reflection
Technical field
The invention belongs to microdisk electrode engineer equipment and technical field, is related to a kind of microalgae training for rotating and being reflected with light Support device.
Background technology
As the term suggests microalgae generally refers to unicellular alga, that is, the plant of only one cell i.e. small algae, Its species is various, vdiverse in function, is the basis of aquatic ecosystem productivity, and tool has been widely used.Microalgae in waters, It is exactly phytoplankton, its ecological functions is equal to the green plants of land, carries out photosynthesis, is its in aquatic ecosystem His trophic level biology provides most of matter and energy source, is the important feed source of fish production.Particularly, no Congener microalgae has different characteristics, and can specifically synthesize has important medical value, health value, warp with accumulating some The material for value of helping, such as carotenoid, the microalgae of astaxanthin are produced, and oil-rich microalgae, the microalgae of enrichment protein, spiral shell Algae etc. is revolved, is a very big industry for the foregoing culture with important use and economic value microalgae.
In view of the important value and purposes of microalgae, the patented technology about microdisk electrode is numerous, there is as many as thousands of items. As a kind of single celled plant, the culture of microalgae needs to provide suitable illumination, nutritive salt, aquatic environment condition, quite a lot of Patented technology proposed around these problems and demand.For example, to improve the light utilization ratio of microdisk electrode, application number Proposed for 2011203597923,2014103094299,2014205459372 Patents and light harvesting is rotated built in exterior lighting The different illumination thinking such as cover or internal illumination and rotation light;To optimize mixed effect, avoiding microalgae deposition from growing nonparasitically upon another plant The patent such as defect, Application No. 2012207005568,201420668458X, 2015102003583 proposes different rotary side The microdisk electrode mode of formula.Based on microalgae unique ecological functions and feature, in possible future, microdisk electrode is very potential should Necessity and important component of the ecosystem, but mesh are artificially built in the outer space in space development, aerospace journey, turning into Before, there is not yet the outer space is agravic or microgravity environment in, carry out the thinking and Patent design of microdisk electrode.
The content of the invention
Present invention seek to address that above-mentioned have problem in the prior art, it is proposed that a kind of to rotate and with the microalgae of light reflection Culture apparatus, it is adapted to the culture of various microalgaes, is particularly suitable for applied in the outer space environment under agravic or microgravity condition Carry out the culture of microalgae.
The technical scheme is that:
It is a kind of rotate and with light reflection micro algae culturing device, the device include support base, rotating basis, shell body, Light source and charging stirring jet pipe;Rotating basis is positioned at support pedestal top, and the outer ring of rotating basis is rotating part, and it rotates speed Degree and direction of rotation are regulated and controled by control element;Shell body is opaque spherical shell, is fixed on the rotatable portion of rotating basis Divide top, together rotated with rotating basis, its structural strength is enough to ensure that rotating part highest after micro algae culturing liquid is filled it up with turns Safety under fast rotation status, and retain 20% safe clearance.Shell body inwall liner specular reflective material, minute surface is inside, Effect is the light sent in ball interior reflection sphere center position light source;Ventilative and peep hole, the hole are set at the top of shell body Place's covering breathable films are only capable of can not be by liquid by gas, the observation for internal microdisk electrode situation;In shell body Heart position sets light source, and light source is fixed on the center non-rotating part of rotating basis, and accesses supply of electric power, light source by pedestal Outside is kept apart itself and the micro algae culturing liquid in device by transparent Bright Source Protection cover;On the rotating part of rotating basis The even more than two charging stirring jet pipes of arrangement.
Further, the intensity of light source, wavelength are regulated and controled by control element, it is ensured that the light that light source is sent can arrive at mirror Face reflective wall.The diameter of integral spherical culture apparatus should not be too big, and increases the diameter of centre of sphere Bright Source Protection cover, ensures microalgae When culture latter stage microalgae density is higher, the light of light source can also arrive at housing and be reflected, that is to say, that light source to liner The distance of minute surface not should be greater than the numerical value of transparency during microalgae concentration highest.
Further, the rotating speed of the outer ring rotating part of described rotating basis, can keep micro algae culturing liquid in spheroid Under agravic or microgravity condition, aggregation is close on bilayer sphere shell, without loss everywhere.
Further, the charging stirring jet pipe on the rotating part, its material spray speed and direction are adjusted by control element Control, the addition of nutritive salt, nutrient solution can be carried out by this pipe, cultured microalgae liquid can also be carried out and collected.The charge pipe The angle of charging, the flow velocity of charging can adjust, and can be rotated with the rotation of culture spheroid, addition available for feed liquid, Collect, can be used for the inner loop of feed liquid, with reference to the rotational case of rotating part, the stabilization in maintenance micro algae culturing liquid flow field With it is uniform, do not formed microalgae deposition and flocculation aggregation.
Beneficial effects of the present invention are, there is provided rotation and with the micro algae culturing device of light reflection, be suitable for various micro- The culture of algae, its rotating basis drive spherical shell to rotate, and then the rotation for the viscosity drive whole beer for passing through liquid, its The inertia that is formed of centrifugal force is rotated, all nutrient solutions can be gathered in using minute surface inner casing as on the spheroid at bottom, to a certain extent The effect of " simulated gravity " can be served as, so as under the agravic or microgravity condition suitable for the outer space, aerospace way Microdisk electrode work.The charge pipe of bottom base, in addition to addition nutrient solution and nutritive salt, it can also carry out the circulation of nutrient solution Injection and agitation, with reference to the rotation of spheroid and pedestal, maintain the outer space agravic or microgravity condition under microdisk electrode flow field The stability of environment, avoid flocculation and the deposition of microalgae.Another feature of the present invention is that spheroid liner mirror-reflection housing can The light launched with reflection source in all directions, and unlimited multiple roundtrip can be carried out in ball interior, until Light particle is utilized and absorbed by microalgae progress photosynthesis.That is, the spherical liner mirror-reflection microalgae training of the present invention Device is supported, equivalent to the black matrix of a light, in addition to the observation air-vent of upper end fritter, light source is launched at the internal centre of sphere Light can all be absorbed by microdisk electrode photosynthesis, and without the excessive of light, therefore, optical energy utilization efficiency is able to pole Big raising.
Brief description of the drawings
Fig. 1 is that the present invention rotates and carries light reflecting device micro algae culturing device,
In figure:1 ventilative and peep hole;The light of 2 liner mirror-reflections;3 shell bodies;4 internal layer shells;The transmitting of 5 light sources Light;6 Bright Source Protection covers;7 light sources;8 microdisk electrode spaces;9 charging stirring jet pipes;10 rotating basis;11 support bases.
Embodiment
Describe the embodiment of the present invention in detail below in conjunction with technical scheme and accompanying drawing.
The present invention's rotates and carries a double-layer ball by rotating basis 10 with the micro algae culturing device of light reflection, main body Shape housing and its interior facility and form.Spherical arrangement epimere is transparent observing and air-vent 1, can not spilt over loss gas Liquid, in addition to overview function, have gas escape function concurrently.The spherical culture apparatus shell 3 of outer layer is made up of opaque material, has Enough structural strengths, culture apparatus can be born and fill nutrient solution under maximum speed rotational case, nutrient solution is for housing The pressure that is formed of centrifugal inertial force, and retain enough safe clearances.Internal layer shell 4 is close to above shell body 3 Specular reflective material, for the roundtrip of ball interior light, form light black matrix effect during microdisk electrode.Above-mentioned bilayer Housing is arranged on the rotating part of rotating base 10, and so as to unitary rotation, rotating speed and steering are adjustable.Inside spherical device It is lighting source 7 and Bright Source Protection cover 6, lighting device is arranged on non-rotating part, is powered by pedestal, but illuminates dress Put and its power pack fixation is not rotatable, separated with pedestal pivotable parts.Pivotable parts set a circle on pedestal Charging agitation jet pipe 9, quantity are no less than two, and charging agitation jet pipe 9 is located at pedestal pivotable parts, moreover, charging agitation spray The charging rate and angle adjustable of pipe 9, adjusted with reference to the rotation of single unit system, micro algae culturing liquid flow field is steady in maintenance spheroid Qualitative and uniformity, flocculation and the deposition of microalgae are avoided, improve microdisk electrode effect.To reach foregoing advantages, charging agitation spray Pipe 9 can carry out the inner loop of micro algae culturing liquid when without the collection of charging and micro algae culturing liquid, and both chargings of half are stirred Dynamic jet pipe suction internal media, second half charging agitation jet pipe nutrient solution that internally injection suction comes simultaneously, forms training The interior flow field circulation of nutrient solution.
The present invention is not meant to be limited to by schematic diagram and specification, without departing from the technology of the present invention principle and its ancestor It can be varied from the premise of purport.Although the present invention be mainly for the outer space is agravic or microgravity environment under the conditions of one Kind microdisk electrode equipment technology, but the microdisk electrode for having under gravity condition on the earth is equally applicable to, the shell of rotation is advantageous to The flow field of micro algae culturing liquid is stable with being well mixed, and the light that liner specular-reflection unit is advantageous to improve microdisk electrode utilizes effect Rate, there is good application effect, be all advantageous for the unique design of microdisk electrode.There is embodiment as after.
Embodiment 1, chlorella is cultivated using the present invention.
A micro algae culturing device of the invention is taken, by device washing and sterilizing, removes dirt caused by other microorganisms possibility Dye, the bead algae culturing liquid that 3/4 volume of addition has configured, the another chlorella algae seedling solution for adding 1/4 volume, control culture water temperature exist 25 degrees centigrades, the intensity of illumination of spherical source is adjusted in 10000 luxs or so.Start spinfunction and the bottom of the present apparatus The agitating function of portion's charging sparge pipe, maintains the well mixed of bead algae culturing liquid, does not form precipitation and flocculation algae group.Before maintenance Suitable cultivation conditions are stated, after 3-4 days, chlorella density can increase to the standard of harvest.Released by bottom feed sparge pipe The algae solution of 1/2-2/3 volumes is harvested, and is left the algae solution of 1/2-1/3 volumes as algae kind, is continued to add 1/2-2/3 volumes Bead algae culturing liquid, foregoing condition of culture is maintained to can proceed with the culture of chlorella.Aforementioned process is repeated, chlorella can be carried out Continuous culture production.
Embodiment 2, spirulina is cultivated using the present invention.
A micro algae culturing device of the invention is taken, by device washing and sterilizing, removes dirt caused by other microorganisms possibility Dye, the spirulina medium that 2/3 volume of addition has configured, the another spirulina algae seedling solution for adding 1/3 volume, control culture water temperature exist 30 degrees centigrades, the intensity of illumination of spherical source is adjusted in 30000 luxs or so.Start spinfunction and the bottom of the present apparatus The agitating function of portion's charging sparge pipe, maintains the well mixed of spirulina medium, does not form precipitation and flocculation algae group.Before maintenance Suitable cultivation conditions are stated, after 2-4 days, spirulina density can grow the standard for increasing to harvest.Pass through bottom feed sparge pipe The algae solution for releasing 1/2 volume is harvested, and is left the algae solution of 1/2 volume as algae kind, is continued to add the spirulina training of 1/2 volume Nutrient solution, foregoing condition of culture is maintained to can proceed with the culture of spirulina.Aforementioned process is repeated, the continuous training of spirulina can be carried out Health is produced.

Claims (5)

1. a kind of rotate and with the micro algae culturing device of light reflection, it is characterised in that the device includes support base, rotation base Seat, shell body, light source and charging stirring jet pipe;For rotating basis positioned at support pedestal top, the outer ring of rotating basis is rotation section Point, its rotary speed and direction of rotation are regulated and controled by control element;Shell body is opaque spherical shell, is fixed on rotation base Seat pivotable parts top, is together rotated, shell body inwall liner specular reflective material, minute surface is inside, shell with rotating basis Ventilative and peep hole is set at the top of body, and covering breathable films at the hole is only capable of that by gas liquid can not be passed through;In shell body Heart position sets light source, and light source is fixed on the center non-rotating part of rotating basis, and accesses supply of electric power, light source by pedestal Outside is kept apart itself and the micro algae culturing liquid in device by transparent Bright Source Protection cover;On the rotating part of rotating basis The even more than two charging stirring jet pipes of arrangement.
2. a kind of micro algae culturing device for rotating and being reflected with light according to claim 1, is further characterized in that, light source Intensity, wavelength regulated and controled by control element, it is ensured that the light that light source is sent can arrive at mirror-reflection wall.
3. a kind of micro algae culturing device for rotating and being reflected with light according to claim 1 or 2, is further characterized in that, The rotating speed of the outer ring rotating part of described rotating basis, micro algae culturing liquid can be kept in spheroid in agravic or microgravity Under the conditions of, aggregation is close on bilayer sphere shell, without loss everywhere.
4. a kind of micro algae culturing device for rotating and being reflected with light according to claim 1 or 2, is further characterized in that, Charging stirring jet pipe on the rotating part, its material spray speed and direction are regulated and controled by control element.
5. a kind of micro algae culturing device for rotating and being reflected with light according to claim 3, is further characterized in that, described Charging stirring jet pipe on rotating part, its material spray speed and direction are regulated and controled by control element.
CN201710821733.5A 2017-06-06 2017-09-15 Rotary microalgae culture device with light reflection Active CN107446801B (en)

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CN201710415975 2017-06-06
CN2017104159754 2017-06-06

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207342A (en) * 2018-08-15 2019-01-15 福清市新大泽螺旋藻有限公司 A kind of spirulina ecological cultivation system
CN114540162A (en) * 2022-03-24 2022-05-27 衢州学院 Closed microalgae culture device
CN115176711A (en) * 2021-04-06 2022-10-14 航天神舟生物科技集团有限公司 Multipurpose space biological experiment device with variable gravity

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1262706A (en) * 1998-03-31 2000-08-09 有限公司迈可罗凯雅 Fine algae culture device
CN102071134A (en) * 2010-12-11 2011-05-25 福州大学 Spirulina light emitting diode (LED) photobioreactor
CN102250756A (en) * 2011-06-30 2011-11-23 农业部规划设计研究院 Divergent cold light source vertical microalgae culture photoreactor
CN204097469U (en) * 2014-09-22 2015-01-14 新奥科技发展有限公司 Micro-algae Immobilized culture device
CN206814753U (en) * 2017-06-06 2017-12-29 南京追梦生物科技有限公司 It is a kind of to rotate and with the micro algae culturing device of light reflection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1262706A (en) * 1998-03-31 2000-08-09 有限公司迈可罗凯雅 Fine algae culture device
CN102071134A (en) * 2010-12-11 2011-05-25 福州大学 Spirulina light emitting diode (LED) photobioreactor
CN102250756A (en) * 2011-06-30 2011-11-23 农业部规划设计研究院 Divergent cold light source vertical microalgae culture photoreactor
CN204097469U (en) * 2014-09-22 2015-01-14 新奥科技发展有限公司 Micro-algae Immobilized culture device
CN206814753U (en) * 2017-06-06 2017-12-29 南京追梦生物科技有限公司 It is a kind of to rotate and with the micro algae culturing device of light reflection

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207342A (en) * 2018-08-15 2019-01-15 福清市新大泽螺旋藻有限公司 A kind of spirulina ecological cultivation system
CN109207342B (en) * 2018-08-15 2021-09-03 福清市新大泽螺旋藻有限公司 Ecological breeding system of spirulina
CN115176711A (en) * 2021-04-06 2022-10-14 航天神舟生物科技集团有限公司 Multipurpose space biological experiment device with variable gravity
CN114540162A (en) * 2022-03-24 2022-05-27 衢州学院 Closed microalgae culture device

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