CN107439936A - Polypeptide Diatotherapeutic flour of diabetic and preparation method thereof - Google Patents
Polypeptide Diatotherapeutic flour of diabetic and preparation method thereof Download PDFInfo
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- CN107439936A CN107439936A CN201710832536.3A CN201710832536A CN107439936A CN 107439936 A CN107439936 A CN 107439936A CN 201710832536 A CN201710832536 A CN 201710832536A CN 107439936 A CN107439936 A CN 107439936A
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- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000030136 gastric emptying Effects 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
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- 230000005764 inhibitory process Effects 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
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- 239000011777 magnesium Substances 0.000 description 1
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- 230000012666 negative regulation of transcription by glucose Effects 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 238000003305 oral gavage Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 230000001175 peptic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
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- 235000019192 riboflavin Nutrition 0.000 description 1
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- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
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- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- REJLGAUYTKNVJM-SGXCCWNXSA-N tomatine Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)O)O[C@@H]1C[C@@H]2CC[C@H]3[C@@H]4C[C@H]5[C@@H]([C@]4(CC[C@@H]3[C@@]2(C)CC1)C)[C@@H]([C@@]1(NC[C@@H](C)CC1)O5)C)[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O REJLGAUYTKNVJM-SGXCCWNXSA-N 0.000 description 1
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- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
- A23L7/107—Addition or treatment with enzymes not combined with fermentation with microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention provides polypeptide Diatotherapeutic flour of a kind of diabetic and preparation method thereof, belong to field of food.The polypeptide Diatotherapeutic flour of the diabetic includes the following raw material in terms of parts by weight：10~40 portions of wheat flours, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts of inulin, 5~10 parts of cucumber powders or tomato meal, 1~5 portion of konjaku flour, 1~10 part of navy bean powder, 5~15 parts of Kelp Powders, 0~5 part of nutrient yeast powder, 0.01~0.05 complex enzyme formulation.The polypeptide Diatotherapeutic flour of diabetic provided by the invention is rich in hypoglycemic bitter gourd polypeptide albumen, the plant polyose of effective extraction purification, and dietary fiber content is high, the postprandial blood sugar of diabetic is not only effectively reduced through zoopery and test-meal, long-term consumption can improve impaired islet function, improve the quality of life of diabetic.Present invention also offers the preparation method of the polypeptide Diatotherapeutic flour of above-mentioned diabetic.
Description
Technical field
The present invention relates to field of food, in particular to the polypeptide Diatotherapeutic flour of diabetic a kind of and its preparation
Method.
Background technology
Diabetes are due to defect of insulin secretion or the impaired caused metabolism characterized by hyperglycaemia of its biological agent
Property disease, seriously endanger to people health and daily life.China diabetic is up to 1.14 hundred million at present, and suffers from
Patient's number is also in cumulative year after year.The harm of diabetes is huge, and also patient is good for diabetic complication caused by diabetes
Health and life constitute a threat to.Diabetes Gu is referred to as quenching one's thirst, and is due to that yin deficiency is scorching, caused by deficient five internal organs to drink more, diuresis,
Eat and become thin, be tired more, the syndrome that urine sweet tea is principal character.Modern medicine study shows that the generation of diabetes is mainly
Because islet cells is impaired, hypoinsulinism or body function disorder cause body to decline insulin sensitivity, from
And the function of regulation blood glucose can not be played and cause diabetes.
Momordica Curcurbitaceae balsam pear platymiscium, bitter is cold in nature, is a kind of medicine-food two-purpose vegetables.According to《Compendium of Materia Medica》And《Yunnan
Southern book on Chinese herbal medicine》Record, balsam pear has the function that clearing away heat and removing summer, nourishing blood and invigorating qi, kidney and spleen invigorating, nourishing liver for improving eyesight, hypoglycemic.Patient of diabetes
Person not only needs the long-term use of or hypoglycemic class medicine of injection, it is also necessary to is controlled on diet, such as avoids intake height from containing
The food of sugar amount, this also serious diet selection for having influence on diabetic.Flour is the most main of processing flour-made food
One of raw material is wanted, contains the wheat flour specially and flour-made food of balsam pear raw material for diabetic at present.Chinese patent
Document 201510443218.9 discloses diabetes nutrition pantry wheat flour specially, using grain flour as base-material, adds soybean degreasing
Deng dietary fibers such as vegetable protein, soyabean tissue's separation, obtained by physical mixing processes；Patent document 201410336288.X
A kind of diabetes patient's special health flour and preparation method thereof is disclosed, various health products are divided with plant and part nutrition food materials
Not Jin Ti, filtering and concentrating dry after mix；Patent document 201510551759.3 disclose a kind of diabetes patient's flour and its
Processing and treating method, flour are made up of edible flour and Chinese medicine.
Although it has been recognized that balsam pear is a kind of medicine-food two-purpose vegetables for having effect of lowering blood sugar, and applied to
In dietotherapy, but there are problems that simultaneously：First, in above-mentioned document and flour specially for diabetic containing balsam pear raw material, majority will
Balsam pear directly crushes or squeezed the juice utilization, or core mass composition is unclear after balsam pear extraction, and modern biomedical research shows,
High activity bitter gourd polypeptide albumen is the key component of Effective Regulation metabolism of blood glucose.Second, Chinese medicine is on the high side, and meals class activity is more
Sugar, water-soluble dietary fiber and vitamins and other nutritious components deficiency, it is difficult to meet diabetes patient long-term consumption with reach improve by
Damage the needs of islet cell function.Therefore, it is necessary to which a kind of nutritious and postprandial blood sugar generation index is low, long-term consumption safety is simultaneously
The bitter gourd polypeptide flour of food therapy effect is can reach to diabetic.
The content of the invention
The invention provides the polypeptide Diatotherapeutic flour of diabetic a kind of, the bitter gourd polypeptide egg rich in effective extraction purification
In vain, plant polyose, and water soluble dietary fiber content is high, has flavor nutrition all good, and postprandial blood sugar generation exponential quantity is low, and
The advantages of diabetes blood glucose's metabolism can effectively be adjusted.
Present invention also offers a kind of preparation method of the polypeptide Diatotherapeutic flour of diabetic, and it has technical matters steady
Fixed, quality controllability is strong, prepares the flour for being suitable to diabetes patient's long-term consumption.
What the present invention was realized in：
The polypeptide Diatotherapeutic flour of diabetic a kind of, in terms of parts by weight, it includes following raw material：10~40 portions of wheats
Powder, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts of inulin, 5~10 parts of cucumber powders or tomato meal, 1~5 part of konjaku flour, 0.01
~0.05 part of complex enzyme formulation.
Further, in present pre-ferred embodiments, in terms of parts by weight, raw material includes：25~40 parts of wheat flours, 15
~25 parts of bitter gourd polypeptide extracts, 15~20 parts of inulin, 6~8 parts of cucumber powders or tomato meal, 3~4 portions of konjaku flours, 0.01~
0.02 part of complex enzyme formulation.
Further, in present pre-ferred embodiments, in terms of parts by weight, raw material also include 0~10 part of navy bean powder,
0~15 part of Kelp Powder, 0~5 part of nutrient yeast powder.
Further, in present pre-ferred embodiments, the grape that complex enzyme formulation includes 200-300U/kg is glycoxidative
Enzyme, 300-500U/kg lipoxygenase, 200-300U/kg zytase, 100-250U/kg glutamine transaminage
In one or more.
Further, in present pre-ferred embodiments, the polypeptide protein content in bitter gourd polypeptide extract is 25%
More than, for the molecular weight distribution of polypeptide protein between 4.5k~68k, its middle-molecular-weihydroxyethyl is 5.5~7kDa bitter gourd polypeptide fragment
Content accounting example be 18~25%.
Further, in present pre-ferred embodiments, it is small that wheat flour includes high gluten wheat flour, medium-strength wheat flour or low muscle
At least one of flour is several.
Further, in present pre-ferred embodiments, the protein content of navy bean powder is more than 60%, its alphalise starch
Enzyme inhibition activity is not less than 1000u/g；The protein content of nutrient yeast powder is not less than 50%.
Present invention also offers a kind of preparation method of the polypeptide Diatotherapeutic flour of diabetic, it comprises the following steps：
In terms of parts by weight, by 10~40 portions of wheat flours, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts of inulin, 5~10 portions of cucumber
Powder or tomato meal, 1~5 portion of konjaku flour cross 150-200 mesh sieves after mixing and obtain mixed powder, dry, then add 0.01~0.05 part
Complex enzyme formulation powder, it is well mixed to obtain polypeptide Diatotherapeutic flour.
Further, in present pre-ferred embodiments, the drying of mixed powder uses 55~85 DEG C of gas, controls moisture
Content is reduced to 9.5~12%.
Further, in present pre-ferred embodiments, the preparation method of bitter gourd polypeptide extract is：Balsam pear raw material is clear
Wash, be beaten broken, balsam pear raw materials are extracted at 38~42 DEG C with purified water, then centrifuge and remove slag, obtain centrifugate；By centrifugate by cutting
Three organic tube type filter membrance systems in parallel for staying molecular weight to be 0.1 μm~3 μm are filtered, and are removed big molecular impurity, are obtained
To microfiltration membranes permeate；Microfiltration membranes permeate is purified by two rolling ultrafiltration membranes in parallel that molecular cut off is 150kDa
System is filtered, and obtains milipore filter permeate；It it is again two of 1000Da in parallel through molecular cut off by milipore filter permeate
Rolling ultrafiltration membrane purification system is filtered, and obtains milipore filter trapped fluid；Trapped fluid is passed through the four of molecular cut off >=150Da
The rolling high pressure reverse osmosis membrane system of branch series connection is concentrated, and is removed moisture and part residual inorganic salts and small molecular weight impurity, is obtained
To concentrate, solid content >=30% of concentrate；Concentrate is dried, obtains polypeptide protein content not less than 25%
Bitter gourd polypeptide extract.
The beneficial effects of the invention are as follows：The embodiments of the invention provide the polypeptide Diatotherapeutic flour of diabetic a kind of, its
Including the following raw material in terms of parts by weight：10~40 parts of medium-strength wheat flours, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts
Inulin, 5~10 parts of cucumber powders or tomato meal, 1~5 portion of konjaku flour, 0.01~0.05 part of complex enzyme formulation and 0~10 part are white
Kidney bean powder, 0~15 part of Kelp Powder, 0~5 part of nutrient yeast powder.The polypeptide Diatotherapeutic flour of diabetic provided by the invention, it is rich
Bitter gourd polypeptide albumen, plant polyose containing effective extraction purification, and water soluble dietary fiber content is high, unique strong cucumber or
Tomato flavor, postprandial blood sugar generation exponential quantity is low, and can effectively adjust diabetes blood glucose's metabolism, improves diabetic's
Quality of life.
Present invention also offers the preparation method of the polypeptide Diatotherapeutic flour of above-mentioned diabetic, including bitter gourd polypeptide to extract
The preparation method of thing, multi-level membrane separation and purification technology ensure that the high activity and content of natural balsam pear polypeptide protein.The party
Law technology process stabilizing, quality controllability are strong.Complex enzyme formulation is added in the preparation technology of flour, the machinery for improving dough adds
Work performance, the quality for improving processing Flour product, i.e. texture and mouthfeel.The polypeptide Diatotherapeutic flour is nutritious, shelf lives length, fits
In the staple food flour being eaten for a long time as diabetes patient.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below by embodiment it is required use it is attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not construed as pair
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is the SDS-PAGE electrophoresis of polypeptide protein molecular weight distribution in bitter gourd polypeptide extract in the embodiment of the present invention
Figure.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional production that can be obtained by commercially available purchase
Product.
The embodiment of the present invention is specifically described below.
The polypeptide Diatotherapeutic flour of diabetic a kind of is present embodiments provided, in terms of parts by weight, it includes following original
Material：10~40 portions of wheat flours, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts of inulin, 5~10 parts of cucumber powders or tomato meal, 1
~5 portions of konjaku flours, 0.01~0.05 part of complex enzyme formulation.Preferably, raw material can also include：25~40 portions of wheat flours, 15~
25 parts of bitter gourd polypeptide extracts, 15~20 parts of inulin, 6~8 parts of cucumber powders or tomato meal, 3~4 parts of konjaku flours, 0.01~0.02
Part complex enzyme formulation.Further preferably, raw material also includes 0~10 part of navy bean powder, 0~15 part of Kelp Powder, 0~5 part of nutritious yeast
Powder；It is further preferred that complex enzyme formulation includes 200-300U/kg glucose oxidase, 300-500U/kg lipoxidases
One or more in enzyme, 200-300U/kg zytases, 100-250U/kg glutamine transaminages；It is further preferred that
Polypeptide protein content in bitter gourd polypeptide extract more than 25%, the molecular weight distribution of polypeptide protein 4.5k~68k it
Between, the content accounting example for the bitter gourd polypeptide fragment that its middle-molecular-weihydroxyethyl is 5.5~7kDa is 18~25%.It is it is further preferred that small
Flour according to the edible processing purposes of flour, can be at least one of high gluten wheat flour, medium-strength wheat flour or low-gluten wheat flour or
Several mixed powders.It is further preferred that the protein content of navy bean powder, more than 60%, its alpha-amylase inhibitory activity is not low
In 1000u/g；Nutrient yeast powder is rich in vitamin and mineral, and its protein content is not less than 50%.
The polypeptide Diatotherapeutic flour of diabetic provided in an embodiment of the present invention using wheat flour, bitter gourd polypeptide extract,
Inulin, cucumber powder or tomato meal, konjaku flour, navy bean powder, Kelp Powder, nutrient yeast powder, complex enzyme formulation powder coordinate, and can make
It is standby to obtain that flavor nutrition is all good, postprandial blood sugar generation exponential quantity is low, and can effectively adjust the dietotherapy of diabetes blood glucose's metabolism
Flour.Due to adding complex enzyme formulation, improve the machining property of dough, improve the quality of processing Flour product, i.e. texture
And mouthfeel.Wherein：
Bitter gourd polypeptide extract is the product for extracting to obtain from balsam pear.The bitter gourd polypeptide contained in balsam pear has drop blood
The effect of sugar, reducing blood lipid, lifting body immunity, blood glucose and glycosuria, prevention and auxiliary treatment diabetes can be effectively reduced.
The molecular weight of the bitter gourd polypeptide contained in bitter gourd polypeptide extract between 5k~60k based on, its middle-molecular-weihydroxyethyl is 5.5~7kDa
Bitter gourd polypeptide fragment content accounting example be 18~25%.These bitter gourd polypeptide clip sizes close to insulin molecular weight,
It is strong with the binding ability of insulin receptor, insulin receptor protein signal path can be effectively activated, recovers insulin function, enters
And blood sugar recovery effectively can be adjusted and control to normal level.
Wheat flour rich in the nutrient such as starch, protein, fat, while also containing the mineral matters such as calcium, iron, thiamine,
The vitamin such as riboflavin, nicotinic acid and vitamin A and vitamin C, it is to meet that the staple food of diabetic's nutrient energy demand is former
Material；
Inulin is a kind of water-soluble dietary fiber, and it will not be hydrolyzed into monose on the top of enteron aisle, thus will not raise
Blood sugar level and insulin content, inulin can significantly reduce the blood glucose value of user, and improve the gastro-intestinal digestion work(of patient
Energy, improve the metabolism of patient.
Cucumber contains abundant cucumber polysaccharide, pectin, amino acid and mineral matter, has a special flavor, and wherein cucumber is more
Sugar can improve body's immunity as nonspecific immunity strengthening agent；Cucumber contains abundant cobalt, and it can enliven the new of human body
Old metabolism, promote hematopoiesis function, and in participant's body vitamin B12 synthesis, be micro member necessary to human islet cell
Element.
" tomatin " that tomato contains plays the role of to suppress bacterium, while its malic acid, citric acid and carbohydrate energy for containing
The effect of gastrointestinal function is enough adjusted, peptic function, tomato is rich in vitamin A, vitamin C, vitamin B1, vitamin B2
And the multiple element such as carrotene and calcium, phosphorus, potassium, magnesium, iron, zinc, copper and iodine, also containing protein, carbohydrate, organic acid, fiber
Element, it is the food that a kind of unique flavor is adapted to diabetes patient again.
Navy bean is that a kind of natural starch blocks food, and navy bean is rich in alpha-amylase inhibitor and dietary fiber, its
The decomposition of starch in high starch food (rice, wheaten food, coarse cereals and related snacks and cake) can be effectively blocked, reduces human body
Glucose absorption, the elevated effect of postprandial blood sugar is reduced so as to play, can effectively coordinate the diet of diabetes patient and slimmer
Treatment, makes them postprandial blood sugar is not raised, body weight does not increase on the premise of having enough.
Konjaku can adjust the absorption rate of food in stomach, make sugar rich in abundant water-soluble dietary fiber SKGM
Class, which absorbs, to be slowed down, and postpones the emptying of food in stomach, and blood glucose rises after meal for control；Polysaccharide can also be with internal unnecessary cholesterol knot
Close, reduce cholesterol absorption, cholesterolemia concentration declines.
The algal polysaccharides that kelp Rich contains can delay the time of gastric emptying and food by small intestine, ensure in amount of insulin secretion
In the case of reduction, blood-sugar content will not also rise, and play a part of adjusting blood glucose.Mushroom contains abundant protein, polysaccharide
And multivitamin, it has the function that to improve immunity of organisms, anti-oxidant and regulation blood sugar for human body.
For nutritious yeast rich in abundant minerals and vitamins, it is body fat and glycometabolism to be especially enriched in B family vitamin
Necessary material, it can improve body immunity and prevent and treat vascular diseases caused by diabetic complication.
A kind of preparation method of the polypeptide Diatotherapeutic flour of diabetic, it comprises the following steps：, will in terms of parts by weight
10~40 portions of wheat flours, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts of inulin, 5~10 parts of cucumber powders or tomato meal, 1~5
Part konjaku flour, 0~10 part of navy bean powder, 0~15 part of Kelp Powder cross 150-200 mesh sieves after mixing and obtain mixed powder, dry；It is preferred that
, the drying of mixed powder uses 55~85 DEG C of gas, and control moisture is reduced to 9.5~12%.Then 0-5 parts battalion is added
Dusty yeast and 0.01~0.05 part of complex enzyme formulation powder are supported, it is well mixed to obtain polypeptide Diatotherapeutic flour.
The preparation method of the polypeptide Diatotherapeutic flour of diabetic provided in an embodiment of the present invention is by the hardship of hypoglycemic activity
Melon polypeptide protein, plant polyose, water-soluble dietary fiber and nutritious yeast etc. compound, and unique strong cucumber or tomato wind
Taste；The technical matters of this method is stable, and quality controllability is strong.Complex enzyme formulation is added in the preparation technology of flour, improves face
The machining property of group, the quality for improving processing Flour product, i.e. texture and mouthfeel.Flour moisture is controlled simultaneously, is improved
The holding time of flour, flour is avoided to be lumpd in humidity.
Wherein, the preparation method of bitter gourd polypeptide extract is：
S1, cleaning：From fresh bitter or dry balsam pear, bitter melon seed be used as balsam pear raw material, more select growth period it is longer, it is green
The fresh bitter that colour cast is yellow, balsam pear seed is fuller is as raw material.Balsam pear cleaning method be：By balsam pear raw material first through running water
Cleaning, then cleaned through electrolytic high-concentration ozone water, further to remove agriculture residual pesticide, microorganism residual etc..
S2, mashing or crushing：20 mesh sieves were ground into by the fresh bitter mashing after cleaning or by dry balsam pear raw material
Coarse powder.
S3, extraction：Balsam pear raw material after mashing or crushing is poured into Ultrasonic Wave-Assisted Extraction tank, and adds 5~10 mass
Times, 38~42 DEG C of purified water, using buffer salt regulation system pH=6.8~7 be neutrality, carry out adverse current extraction 2~4 times, often
Secondary 30~60 minutes.
S4, centrifugation are removed slag：Centrifuged, and removed slag using disc centrifuge or tube centrifuge, obtain centrifugate.
S5, micro-filtration：Centrifugate is filtered by the microfiltration membrane system that molecular cut off is 3 μm~0.1 μm, removed big
Molecular impurity, obtain microfiltration membranes permeate.
In the present embodiment, micro-filtrate membrane filtration system is used in parallel by three films, and filter membrane is organic tubular type, doughnut
Or ceramic micro filter film composition；The operation temperature filtered by microfiltration membrane system is 15~35 DEG C, operating pressure is 0.1~
0.5MPa。
S6, ultrafiltration：Rolling ultrafiltration membrane purification system by microfiltration membranes permeate by molecular cut off for 50~200kDa
Filtered, obtain milipore filter permeate.
Above-mentioned milipore filter permeate is carried out by the rolling ultrafiltration membrane purification system that molecular cut off is 500~1000Da
Filtering, obtains milipore filter trapped fluid.
In the present embodiment, rolling ultrafiltration membrane purification system is used in parallel using two films, and filter membrane is to contain (PS) polysulfones
Or the filter membrane of (PFS) polyether sulfone materials.The operation temperature purified by rolling ultrafiltration membrane purification system is 15~35 DEG C,
Operating pressure is 0.3~0.8MPa.
S7：Nanofiltration：Trapped fluid is concentrated by molecular cut off >=100Da rolling high pressure reverse osmosis membrane, to cut
The molecule for staying molecular weight to be not less than 100Da, molecule of the molecular weight less than 100Da is by removing, removal partial moisture and salt ion,
Obtain molecular weight >=100Da concentrate, solid content >=30% of concentrate.The present embodiment is also an option that retention molecule
Amount >=150Da rolling high pressure reverse osmosis membrane is concentrated.
In the present embodiment, rolling high pressure reverse osmosis membrane is high pressure concentration film, specifically by polyamide compoiste material film system
Into.Rolling high pressure reverse osmosis membrane system concentration systems are used using four film cascades.Enter by rolling high pressure reverse osmosis membrane system
The operation temperature of row concentration is 15~35 DEG C, and operating pressure is 3.5~5.5MPa.
S8, drying：Concentrate is dried, drying means is vacuum freeze drying or low temperature spray drying, obtains hardship
Melon polypeptide protein content is not less than 25% Bitter Melon P.E powder, as bitter gourd polypeptide extract.
The preparation method of the polypeptide dietotherapy noodles of diabetic provided in an embodiment of the present invention uses membrane separating and purifying skill
Art obtains bitter gourd polypeptide extract, and this method exempts enzymolysis and high temperature enzyme deactivation step, with short production cycle, isolates and purifies thickening temperature
It is low, the natural activity and its high content of the bitter melon protein polypeptide prepared is effectively ensured, balsam pear in bitter gourd polypeptide protein extract
The content of polypeptide protein (molecular weight is 4.5k~68kDa) is at least above 25%, 5.5~7kDa of its middle-molecular-weihydroxyethyl bitter gourd polypeptide
The content accounting example of fragment is 20%.These bitter gourd polypeptide clip sizes have regulation and control close to the molecular weight of insulin
Blood glucose function.
The feature and performance of the inventive method are described in further detail with reference to embodiments.
Embodiment 1
Embodiment 1 provides the polypeptide Diatotherapeutic flour of diabetic a kind of, and its specific preparation process is as follows：
Prepare bitter gourd polypeptide extract：Longer from growth period, the fresh bitter that green is partially yellow, balsam pear seed is fuller is made
For raw material, balsam pear raw material is first cleaned through running water, then cleaned through electrolytic high-concentration ozone water, cleans Measuring Ozone Concentration in Water
15mg/L, water-carrying capacity 300L/h, the balsam pear raw material pulping after cleaning was broken into the liquid material of 20 eye mesh screens；Mashing is broken
Balsam pear raw material after broken pours into Ultrasonic Wave-Assisted Extraction tank, and adds 8 mass times, 38 DEG C of purified water, is adjusted using buffer salt
System pH ≈ 7, the adverse current for carrying out ultrasonic wave added are extracted 3 times, every time 30 minutes.Centrifuged using high speed tube centrifuge, and
Remove slag, obtain centrifugate.Centrifugate is filtered by the hollow fiber microfiltration membrane system that molecular cut off is 1 μm, operation temperature
Spend for 30 DEG C, operating pressure 0.3MPa, obtain microfiltration membranes permeate.It is by molecular cut off respectively by microfiltration membranes permeate first
200kDa rolling ultrafiltration membrane purification system is filtered, and operation temperature is 30 DEG C, operating pressure 0.8MPa, it is saturating to obtain milipore filter
Cross liquid；It will transmit through liquid again to be filtered by the rolling ultrafiltration membrane purification system that molecular cut off is 800Da, operation temperature 30
DEG C, pressure 0.8MPa, obtain milipore filter trapped fluid.Milipore filter trapped fluid is high by the rolling that molecular cut off is 150Da
Pressure reverse osmosis membrane system is concentrated, and operation temperature is 30 DEG C, operating pressure 4MPa, obtains the dense of solid content >=30%
Contracting liquid.Concentrate is dried by the way of vacuum freeze drying, obtains bitter gourd polypeptide protein extract, its polypeptide protein
Content up to 27.5%.
By 600g medium-strength wheat flours, 150g bitter gourd polypeptides extract, 300g inulin, 120g cucumber powders, 80g konjaku flours, 80g
Navy bean powder, 50g Kelp Powders cross 150 mesh sieves after mixing and obtain mixed powder, dry, and using 55 DEG C of gas, control the last water of flour
Divide content to 10.5%.Then 20g nutrient yeast powders are added and 0.1g 300U glucose oxidases, 200U zytases mix
Conjunction uniformly obtains polypeptide Diatotherapeutic flour.
Embodiment 2
Embodiment 2 provides the polypeptide Diatotherapeutic flour of diabetic a kind of, and its specific preparation process is as follows：
Prepare bitter gourd polypeptide extract：Longer from growth period, the fresh bitter that green is partially yellow, balsam pear seed is fuller is made
For raw material, balsam pear raw material is first cleaned through running water, then cleaned through electrolytic high-concentration ozone water, Measuring Ozone Concentration in Water 20mg/
L, water-carrying capacity 450L/h, remained with reducing agriculture residual pesticide and microorganism.Balsam pear raw material pulping after cleaning was broken into 20 mesh
The liquid material of screen cloth.By be beaten it is broken after balsam pear raw material pour into Ultrasonic Wave-Assisted Extraction tank, and add 5 mass times, 40 DEG C
Purified water, using buffer salt regulation system pH ≈ 6.7, the adverse current for carrying out ultrasonic wave added is extracted 2 times, every time 60 minutes.Using
Disc centrifuge is centrifuged, and is removed slag, and obtains centrifugate.Centrifugate is passed through into organic tubular type that molecular cut off is 0.1 μm
Microfiltration membrane system is filtered, and operation temperature is 25 DEG C, pressure 0.5MPa, obtains microfiltration membranes permeate.First microfiltration membranes are passed through
Liquid is filtered by the rolling ultrafiltration membrane purification system that molecular cut off is 100kDa respectively, and operation temperature is 35 DEG C, pressure
For 0.6MPa, milipore filter permeate is obtained；Rolling ultrafiltration membrane purification system of the liquid by molecular cut off for 1000Da is will transmit through again
Filtered, operation temperature is 25 DEG C, pressure 0.8MPa, obtains milipore filter trapped fluid.By milipore filter trapped fluid by retaining
Molecular weight is that 100Da rolling high pressure reverse osmosis membrane system is concentrated, and operation temperature is 25 DEG C, operating pressure 5.0MPa,
Obtain the concentrate of solid content >=30%.Concentrate is dried by the way of low temperature spray drying, obtains balsam pear
Polypeptide extract, the content of its polypeptide protein is 25.8%.
By 1500g high gluten wheat flours, 300g bitter gourd polypeptides extract, 600g inulin, 200g tomato meals, 150g konjaku flours,
150g navy beans powder, 100g Kelp Powders cross 150 mesh sieves after mixing and obtain mixed powder, dry, and using 50 DEG C of gas, control flour is most
Moisture is to 11.0% afterwards.Then 300U glucose oxidases, the 200U wood for adding 40g nutrient yeast powders and 0.15g are poly-
Carbohydrase is well mixed to obtain polypeptide Diatotherapeutic flour.
Embodiment 3
Embodiment 3 provides the polypeptide Diatotherapeutic flour of diabetic a kind of, and its specific preparation process is as follows：
Prepare bitter gourd polypeptide extract：Raw material is used as from the drying balsam pear or balsam pear section that green is partially yellow, meat is full,
Balsam pear raw material was ground into the coarse powder of 20 mesh sieves.Balsam pear raw material after crushing is poured into Ultrasonic Wave-Assisted Extraction tank, and added
10 mass times, 42 DEG C of purified water, using buffer salt regulation system pH ≈ 6.8, the adverse current for carrying out ultrasonic assistant is extracted 2 times,
45 minutes every time.Centrifuged, and removed slag using high speed tube centrifuge, obtain centrifugate.Centrifugate is passed through into molecular cut off
Filtered for 0.2 μm of ceramic micro filter membranous system, operation temperature is 35 DEG C, pressure 0.5MPa, obtains microfiltration membranes permeate.First
Microfiltration membranes permeate is filtered by the rolling ultrafiltration membrane purification system that molecular cut off is 150kDa respectively, operation temperature
For 35 DEG C, pressure 0.5MPa, milipore filter permeate is obtained；Roll-to-roll ultrafiltration of the liquid by molecular cut off for 500Da is will transmit through again
Film purification system is filtered, and operation temperature is 35 DEG C, pressure 0.5MPa, obtains milipore filter trapped fluid.Milipore filter is retained
Liquid is concentrated by the rolling high pressure reverse osmosis membrane system that molecular cut off is 100Da, and operation temperature is 35 DEG C, and pressure is
4.5MPa, obtain the concentrate of solid content >=30%.Will be dense by the way of vacuum freeze drying or low temperature spray drying
Contracting liquid is dried, and obtains bitter gourd polypeptide extract, and the content of its polypeptide protein is up to 26.6%.
By 1200g medium-strength wheat flours, 350g bitter gourd polypeptides extract, 350g inulin, 200g cucumber powders, 120g konjaku flours,
160g navy beans powder, 100g Kelp Powders cross 200 mesh sieves after mixing and obtain mixed powder, dry, and using 50 DEG C of gas, control flour is most
Moisture is to 11.5% afterwards.Then 35g nutrient yeast powders and 0.2g 300U glucose oxidases, 200U xylans are added
Enzyme is well mixed to obtain polypeptide Diatotherapeutic flour.
The molecular weight of bitter gourd polypeptide albumen in bitter gourd polypeptide extract in embodiment 1 is detected：
The bitter gourd polypeptide extract polypeptide protein molecular weight distribution of embodiment 1 is examined using SDS-PAGE analytic approach
Survey, Fig. 1 is the PAGE electrophoretograms detected.
By Fig. 1 it can be found that：The bitter gourd polypeptide albumen of the bitter gourd polypeptide extract obtained according to preparation method of the present invention, point
For the content accounting for the bitter gourd polypeptide that son amount is 5.5~7kDa more than 20%, the Peptide of Protein is Effective Regulation blood glucose generation
The key function composition thanked, close to insulin molecule amount.Therefore, the balsam pear obtained according to the technical matters of the embodiment of the present invention is more
Contain a large amount of natural activity bitter gourd polypeptide albumen with regulation blood glucose function in peptide extract.
The polypeptide Diatotherapeutic flour of the diabetic provided embodiment 1, embodiment 2 and embodiment 3 detects：
From the rat (150g ± 2g) of health, rat is randomly assigned into 4 groups, used by 60mg/kg/day dosage
Twice, diabetes rat is made in Streptozotocin injection rat, then daily Example 1, embodiment 2 and embodiment 3 respectively
In polypeptide dietotherapy noodles crush, add pure water to prepare gavage liquid, the same day prepares, daily oral gavage once, with reference to animal and
The per kilogram of body weight dosage conversions coefficient table of human body, draw and calculate its gavage capacity according to 6g flour of the present invention/kg/d rats.Even
Continue and give tested material 45d, the pure water of the daily gavage equivalent of control group, last day is detected before gavage with gavage half an hour after
The blood glucose value of each group rat model, as a result shown in following Tables 1 and 2.
Influence of the polypeptide Diatotherapeutic flour of table 1 to rat fasting blood-glucose
Influence of the polypeptide Diatotherapeutic flour of table 2 to rat postprandial blood sugar
From Tables 1 and 2, the blood sugar test value of each group rat there was no significant difference (P before experiment>0.05).With compareing
Group is relatively low compared to each group rat blood sugar value rise and abdomen blood glucose value recovers normal, and it is glycemic to show that the polypeptide Diatotherapeutic flour has
It is worth low and regulation blood glucose effect.
In summary, polypeptide Diatotherapeutic flour provided in an embodiment of the present invention is using wheat flour, bitter gourd polypeptide extract, chrysanthemum
Powder, cucumber powder or tomato meal, konjaku flour, complex enzyme formulation and navy bean powder, Kelp Powder, nutrient yeast powder are mixed mixed
Powder is closed, not only unique strong cucumber or tomato flavor, bitter gourd polypeptide albumen, plant polyose rich in effective extraction purification, and
Water soluble dietary fiber content is high, and postprandial blood sugar generation exponential quantity is low, and can effectively adjust diabetes blood glucose's metabolism, improves
The quality of life of diabetic.Present invention also offers the preparation method of aforementioned polypeptides dietotherapy noodles, including bitter gourd polypeptide to carry
The preparation method of thing is taken, multi-level membrane separation and purification technology ensure that the high activity and content of natural balsam pear polypeptide protein.Should
Method and technology process stabilizing, quality controllability are strong.Complex enzyme formulation is added in the preparation technology of flour, improves the machinery of dough
Processing characteristics, the quality for improving processing Flour product, i.e. texture and mouthfeel.The polypeptide Diatotherapeutic flour is nutritious, shelf lives length,
It is adapted as the staple food flour of diabetes patient's long-term consumption.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (10)
1. the polypeptide Diatotherapeutic flour of a kind of diabetic, it is characterised in that in terms of parts by weight, it includes following raw material：
2. the polypeptide Diatotherapeutic flour of diabetic according to claim 1, it is characterised in that in terms of parts by weight, institute
Stating raw material includes：
3. the polypeptide Diatotherapeutic flour of diabetic according to claim 1 or 2, it is characterised in that in terms of parts by weight,
The raw material also includes 0~10 part of navy bean powder, 0~15 part of Kelp Powder, 0~5 part of nutrient yeast powder.
4. the polypeptide Diatotherapeutic flour of diabetic according to claim 1 or 2, it is characterised in that the complex enzyme system
Agent includes 200-300U/kg glucose oxidase, 300-500U/kg lipoxygenase, 200-300U/kg xylan
Enzyme, 100-250U/kg glutamine transaminage in one or more.
5. the polypeptide Diatotherapeutic flour of diabetic according to claim 1 or 2, it is characterised in that the bitter gourd polypeptide
Polypeptide protein content in extract is more than 25%, and the molecular weight distribution of polypeptide protein is between 4.5k~68k, wherein molecule
The content accounting example for measuring the bitter gourd polypeptide fragment for 5.5~7kDa is 18~25%.
6. the polypeptide Diatotherapeutic flour of diabetic according to claim 1 or 2, it is characterised in that the wheat flour bag
Include at least one of high gluten wheat flour, medium-strength wheat flour or low-gluten wheat flour or several.
7. the polypeptide Diatotherapeutic flour of diabetic according to claim 3, it is characterised in that the egg of the navy bean powder
For white matter content more than 60%, its alpha-amylase inhibitory activity is not less than 1000u/g；The protein content of the nutrient yeast powder
Not less than 50%.
8. the preparation method of the polypeptide Diatotherapeutic flour of a kind of diabetic, it is characterised in that it comprises the following steps：With weight
Number meter, by 10~40 portions of wheat flours, 5~35 parts of bitter gourd polypeptide extracts, 10~20 parts of inulin, 5~10 parts of cucumber powders or kind
Eggplant powder, 1~5 portion of konjaku flour cross 150-200 mesh sieves after mixing and obtain mixed powder, dry, then add 0.01~0.05 part of complex enzyme
Preparation powder, it is well mixed to obtain polypeptide Diatotherapeutic flour.
9. the preparation method of the polypeptide Diatotherapeutic flour of diabetic according to claim 8, it is characterised in that mixed powder
Drying use 55~85 DEG C of gas, control moisture is reduced to 9.5~12%.
10. the preparation method of the polypeptide Diatotherapeutic flour of diabetic according to claim 8, it is characterised in that described
The preparation method of bitter gourd polypeptide extract is：The cleaning of balsam pear raw material, mashing is broken, extract balsam pear at 38~42 DEG C with purified water
Raw material, then centrifuge and remove slag, obtain centrifugate；Being three of 0.1 μm~3 μm by molecular cut off by the centrifugate in parallel has
Machine tube type filter membrance system is filtered, and is removed big molecular impurity, is obtained microfiltration membranes permeate；The microfiltration membranes permeate is passed through
Cross two rolling ultrafiltration membrane purification systems in parallel that molecular cut off is 150kDa to be filtered, obtain milipore filter permeate；Will
The milipore filter permeate is filtered through two rolling ultrafiltration membrane purification systems in parallel that molecular cut off is 1000Da again,
Obtain milipore filter trapped fluid；By rolling high pressure counter-infiltration of the trapped fluid by molecular cut off >=150Da four series connection
Membranous system is concentrated, and is removed moisture and part residual inorganic salts and small molecular weight impurity, is obtained concentrate, the concentrate is consolidated
Shape thing content >=30%；The concentrate is dried, bitter gourd polypeptide of the polypeptide protein content not less than 25% is obtained and extracts
Thing.
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Application publication date: 20171208 |