CN107581620A - A kind of compound lozenge of hypoglycemic bitter gourd polypeptide and preparation method thereof - Google Patents
A kind of compound lozenge of hypoglycemic bitter gourd polypeptide and preparation method thereof Download PDFInfo
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Abstract
The invention provides a kind of hypoglycemic compound lozenge of bitter gourd polypeptide and preparation method thereof, belong to nutrition spy meals and field of functional food.The compound lozenge is included in terms of parts by weight：3~5 parts of bitter gourd polypeptide extracts, 2~4 parts of plantation ginseng concentrates, 1~3 part of kudzu root extract, 1~3 part of Rhizoma Polygonati extract and nutritious yeast 0.6~0.8 ‰.Its preparation method includes：Mixed powder will be obtained after drying and screening, sterilization after each raw material of above-mentioned formula ratio and auxiliary materials and mixing and according to the direct compressing dry granulation of specification.The content of bitter gourd polypeptide albumen is more than 20% in its bitter gourd polypeptide extract, and the content accounting example for the bitter gourd polypeptide fragment that its middle-molecular-weihydroxyethyl is 5.5~7kDa is 18~25%, and the insulin receptor binding ability of the polypeptide fragment is extremely strong.The food and medicine consangunity reasonable recipe of the compound lozenge of this bitter gourd polypeptide, the quality controllability of compressing dry granulation is strong, has significant hypoglycemic and regulation and control carbohydrate metabolism to diabetes and its patient's early stage.
Description
Technical field
The present invention relates to nutrition spy meals and field of functional food, is answered in particular to a kind of hypoglycemic bitter gourd polypeptide
Close lozenge and preparation method thereof.
Background technology
As people's living standard improves constantly quick increasing is showed with social economy's fast development, China diabetic
Long situation, number of patients account for global diabetes patient's sum 1/3rd.China's diabetes morbidity is up to 9.6% at present, sugar
Patient is urinated more than 1.1 hundred million, and number of patients is also in cumulative year after year.The harm of diabetes is huge, with caused by diabetes
Diabetic complication also forms grave danger to the health and lives of patient.Diabetes Gu is referred to as quenching one's thirst, and refers to drink more, be more
Urine, eat and become thin, be tired more, urine sweet tea for principal character syndrome, laboratory examination its be mainly characterized by hyperglycaemia and glucose in urine.
It is due to that yin deficiency is scorching to quench one's thirst, caused by deficient five internal organs by drink more, more food, diuresis, body become thin characterized by illness.It is modern
The main reason for medical research shows, diabetes produce is that impaired islet cells, hypoinsulinism or body function are disorderly
Disorderly cause body to decline insulin sensitivity, cause diabetes so as to which the function of regulation blood glucose can not be played.
Balsam pear also known as balsam pear, belong to Curcurbitaceae balsam pear platymiscium, bitter is cold in nature, is a kind of medicine-food two-purpose vegetables.According to《Herbal guiding principle
Mesh》And《The southern regions of the Yunnan Province book on Chinese herbal medicine》Record, balsam pear has the function that clearing away heat and removing summer, nourishing blood and invigorating qi, kidney and spleen invigorating, nourishing liver for improving eyesight, hypoglycemic；
Balsam pear contains trypsin inhibitor, can suppress the secreted protease out of cancer cell, prevent malignant tumour, and be also equipped with
There are reducing blood lipid and blood pressure.The modern biomedical research about balsam pear gradually increases in recent years, Khana reports in 1981
By extracting the composition that molecular weight is 11kDa in balsam pear fruit, seed and tissue, the composition subcutaneous injection is to human body I, II
Patients with type Ⅰ DM has a good hypoglycemic activity, but its it is oral after because of enteron aisle, to destroy activity poor；Liu Xuan in 2003 report by
Bitter melon seed extracts 6.8kDa bitter gourd polypeptide, polypeptide tool trypsin inhibition activity and anti-plant pathogen activity；2012
Year Blum A reports can suppress the type gene expression of 11beta-Hydroxysteroid dehydrogenase I by the crude extract extracted in balsam pear, be used for
Type II diabetes is treated.Chinese patent literature CN106974951A discloses the pharmaceutical composition for the treatment of diabetes, mainly will
The compound such as Western medicine gliclazide and Chinese medicine ginseng, pseudo-ginseng and vitamin.Chinese patent literature CN107095975A discloses one
Kind treatment diabetes prescription and preparation method thereof, 15 kinds of medicinal materials cleanings are drained, pulverized after stir-frying, after boiling water immersion directly
Take.Chinese patent literature CN106975010A discloses a kind of Chinese medicine composition for treating diabetes, containing 14 kinds of Chinese medicines and
The raw material of integration of drinking and medicinal herbs, including the root of kudzu vine, the Radix Astragali and balsam pear etc., it is directly crushed, sieving mixes, encapsulating capsule.
Although it has been recognized that balsam pear is a kind of plant material for having effect of lowering blood sugar, and applied to glycosuria
In the treatment of disease, but there are problems that simultaneously：When above-mentioned document and the Chinese medicine class galenical about treating diabetes, greatly
More by all kinds of formula materials directly pulverize and sieve it is rear it is filling take or directly add water to cook or crush after plus be decocted in water for oral dose, do not close
The quality controllability and its content of key composition, it is impossible to ensure the stability and conspicuousness of product efficacy；Second, existing use balsam pear
The hypoglycemic class product of extract, Bitter Melon P.E are mostly based on saponin(e, are actually " Momordica charantial ", or using enzyme classes
Solution reaction or alkali carries acid is heavy obtains bitter gourd polypeptide protein, but its content is not high and the preparation time cycle is long or some step behaviour
Make temperature height, and the bitter gourd polypeptide of natural activity is the key component of Effective Regulation metabolism of blood glucose.Therefore, it is necessary to which a kind of quality is steady
Determine and there is the bitter gourd preparation product of significant hypoglycemic effect to diabetes and its patient's early stage.
The content of the invention
The first object of the present invention is to provide a kind of compound lozenge of hypoglycemic bitter gourd polypeptide, and it is more that the lozenge contains balsam pear
Peptide, reasonable recipe, hypoglycemic effect are notable, available for diabetes and its effective regulating and controlling blood sugar metabolism of patient early stage.
The second object of the present invention is to provide a kind of preparation method of the compound lozenge of above-mentioned bitter gourd polypeptide, the preparation method
Prepared using the formula of the compound lozenge of above-mentioned bitter gourd polypeptide, preparation method is simple, and quality controllability is strong, ensures product nucleus
Stability and conspicuousness, and be adapted to industrialized production.The product being prepared has that blood sugar decreasing effect is good, stable effect and clothes
The advantages of with facilitating.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
The present invention proposes a kind of hypoglycemic compound lozenge of bitter gourd polypeptide, and it includes：The bitter gourd polypeptide extraction of 3~5 parts by weight
Thing, the content of bitter gourd polypeptide is more than 20% in bitter gourd polypeptide extract；The ginsenoside extract of 2~4 parts by weight, ginseng soap
The content of ginsenoside is more than 10% in glucoside extract；The kudzu root extract of 1~3 parts by weight, general flavone in kudzu root extract
Content more than 50%；The Siberian solomonseal rhizome polysaccharide extract of 1~3 parts by weight, the content of Siberian solomonseal rhizome polysaccharide exists in Siberian solomonseal rhizome polysaccharide extract
More than 15%；And nutritious yeast 0.6~0.8 ‰.
Further, in present pre-ferred embodiments, the content of bitter gourd polypeptide albumen in the bitter gourd polypeptide extract
More than 20%, the content accounting example for the bitter gourd polypeptide fragment that its middle-molecular-weihydroxyethyl is 5.5~7kDa is 18~25%, and the albumen is more
For fragments of peptides size close to insulin molecule amount, insulin receptor binding ability is extremely strong, can activate islet receptor protein signal path
And recover damaged islet cells function, it is the key function composition of Effective Regulation metabolism of blood glucose.
Further, in present pre-ferred embodiments, the ginseng extraction concentrat is by extracting artificial growth five
Year and following ginseng are simultaneously prepared through 5~10 times of concentrations.
Further, in present pre-ferred embodiments, the compound lozenge of bitter gourd polypeptide also includes the auxiliary material of food-grade,
The auxiliary material includes at least one of filler, adhesive, lubricant, disintegrant or flavouring or several.
Further, in present pre-ferred embodiments, the flavouring is selected from xylitol, menthol, sorbose
Any of alcohol or orange syrup.
Further, in present pre-ferred embodiments, described adhesive be selected from microcrystalline cellulose, hydroxypropyl cellulose,
At least one of gelatin, simple syrup or carbomer.
Further, in present pre-ferred embodiments, the preparation method of the bitter gourd polypeptide extract includes：By balsam pear
Raw material cleaning, mashing are broken, with purification flooding balsam pear raw material, then centrifuge and remove slag, obtain centrifugate；By the centrifugate by cutting
The microfiltration membrane system for staying molecular weight to be 3 μm~0.1 μm is filtered, and is removed big molecular impurity, is obtained microfiltration membranes permeate；By institute
State microfiltration membranes permeate to be filtered by the rolling ultrafiltration membrane purification system that molecular cut off is 150kDa, obtain milipore filter transmission
Liquid；The milipore filter permeate is filtered through the rolling ultrafiltration membrane purification system that molecular cut off is 800Da again, surpassed
Membrane retention liquid；The trapped fluid is concentrated by molecular cut off >=100Da rolling high pressure reverse osmosis membrane system, removed
Moisture and part residual inorganic salts and small molecular weight impurity are removed, obtains concentrate, solid content >=30% of the concentrate；Will
The concentrate is dried, and obtains the bitter gourd polypeptide extract powder that polypeptide protein content is not less than 20%.
Further, in present pre-ferred embodiments, the rolling ultrafiltration membrane purification system is made using two film parallel connections
With filter membrane is the filter membrane containing (PS) polysulfones or (PFS) polyether sulfone materials.Purified by roll-to-roll ultrafiltration membranous system
Operation temperature is 15~35 DEG C, and operating pressure is 0.3~0.8MPa.The rolling high pressure reverse osmosis membrane concentration system uses four
Film cascade uses, and the operation temperature concentrated by rolling high pressure reverse osmosis membrane is 15~35 DEG C, and operating pressure is 3.5~
5.5MPa。
Present invention also offers a kind of preparation method of the hypoglycemic compound lozenge of bitter gourd polypeptide, it includes：By the balsam pear
Polypeptide extract, plantation ginseng extraction concentrat, the kudzu root extract, the Rhizoma Polygonati extract and the nutrition ferment
The carrier auxiliary materials such as female and filler, disintegrant, flavouring, adhesive or lubricant are dried after mixing and cross 50 mesh sieves, through quick
Mixed powder is obtained after low-temperature microwave sterilizing or ultraviolet disinfection；By mixed powder according to the direct compressing dry granulation of specification.
Compared with prior art, beneficial effects of the present invention for example including：A kind of hypoglycemic balsam pear provided by the invention is more
Compound lozenge of peptide and preparation method thereof, the traditional Chinese medical science diabetes of modern medicine study and diabetes based on balsam pear hypoglycemic is theoretical,
Reasonable recipe.High efficiency extraction, separation, purifying largely have the specified molecular weight and sequence for stablizing regulating and controlling blood sugar metabolic function first
Natural activity bitter gourd polypeptide, its insulin receptor binding ability is extremely strong, can activate islet receptor protein signal path and recovery
Damaged islet cells function, and then blood sugar recovery effectively can be adjusted and control to normal level.It is unique in addition combined with traditional Chinese medicine
Formula can strengthen the nutrition of the trace element containing chromium etc. of human insulin sensitiveness from American Ginseng, the root of kudzu vine, the Radix Astragali, and addition
Yeast is through being combined, and product quality is controllable and blood sugar decreasing effect is notable, can be effectively used for the blood of diabetes and its patient's early stage
Glycometabolism regulates and controls.The technical matters of the compound lozenge of hypoglycemic bitter gourd polypeptide provided by the invention is stable, and quality controllability is strong, ensures
The stability and conspicuousness of product nucleus, and it is adapted to industrialized production.The compound lozenge tool of bitter gourd polypeptide being prepared
Have that blood sugar decreasing effect is good, stable effect and it is convenient to take the advantages of.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below by embodiment it is required use it is attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not construed as pair
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is the SDS-PAGE electrophoresis of the polypeptide protein molecular weight distribution of bitter gourd polypeptide extract in the embodiment of the present invention
Figure；
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition suggested according to normal condition or manufacturer are carried out.Agents useful for same or the unreceipted production firm person of instrument, it is
The conventional products that can be obtained by commercially available purchase.
Present embodiment provides a kind of hypoglycemic compound lozenge of bitter gourd polypeptide, and the compound lozenge of the bitter gourd polypeptide includes：
The bitter gourd polypeptide extract of 3~5 parts by weight, the ginsenoside extract of 2~4 parts by weight, the Pueraria lobota of 1~3 parts by weight
Root chromocor extract, the Siberian solomonseal rhizome polysaccharide extract of 1~3 parts by weight, nutritious yeast 0.6~0.8 ‰.
Optionally, Polypeptide-k buccal tablet includes：The bitter gourd polypeptide extract of 3.5~4.5 parts by weight, 2.5~3.5 parts by weight
Ginsenoside extract, the Kudzu Flavonoids Extracts of 1.5~2.5 parts by weight, 1.5~2.5 parts by weight Siberian solomonseal rhizome polysaccharide extraction
Thing, nutritious yeast 0.65~0.75 ‰.
More specifically, Polypeptide-k buccal tablet includes：Bitter gourd polypeptide extract, the ginsenoside of 3 parts by weight of 4 parts by weight
Extract, the Kudzu Flavonoids Extracts of 2 parts by weight, the Siberian solomonseal rhizome polysaccharide extract of 2 parts by weight, nutritious yeast 0.7 ‰.
Wherein, bitter gourd polypeptide is extracted from balsam pear and obtained, but because the species of bitter gourd polypeptide is more, molecular weight difference
Greatly, extraction efficiency is low so that the content of bitter gourd polypeptide is relatively low in bitter gourd polypeptide extract in the prior art, and then influences its hypoglycemic
Effect.In the present embodiment, in bitter gourd polypeptide extract the content of bitter gourd polypeptide albumen more than 20%；Wherein, through research
The molecular weight for showing natural balsam pear polypeptide protein is 4.5k~68kDa, and wherein insulin receptor binding ability is extremely strong, Effective Regulation
The molecular weight of the crucial polypeptide of metabolism of blood glucose function divides in 5.5~7kDa, this part bitter gourd polypeptide clip size close to insulin
Son amount, has para-insulin function.Therefore, it is further preferred that bitter gourd polypeptide extract middle-molecular-weihydroxyethyl is 5.5~7kDa hardship
The content accounting example of melon polypeptide is 18~25%, is either 19~24% or is 23%.
Further, the preparation method of this bitter gourd polypeptide extract includes：
A. purification flooding balsam pear raw material is used, then centrifuges and removes slag, obtains centrifugate.
Optionally, it is as balsam pear raw material, the method for cleaning from fresh bitter or dry balsam pear, bitter melon seed：By balsam pear
Raw material first cleans through running water, then is cleaned through electrolytic high-concentration ozone water, cleans Measuring Ozone Concentration in Water up to 10~20mg/L, water
150~450L/h of flow, further to remove agriculture residual pesticide, microorganism residual etc..
Further, included with the method for purification flooding balsam pear raw material：Balsam pear raw material after mashing is broken pours into super
Sound wave Assisted Extraction tank, and add 5~10 mass times, 38~52 DEG C of purified water, using buffer salt regulation system pH=6.8~
7 i.e. neutrality, carry out adverse current extraction 2~4 times, every time 30~60 minutes.
Further, centrifuging the method to remove slag is：Centrifuged using high speed tube centrifuge or disc centrifuge.
B. centrifugate is filtered by the microfiltration membrane system that molecular cut off is 3 μm~0.1 μm, it is miscellaneous removes macromolecular
Matter, obtain microfiltration membranes permeate.Molecular cut off is that 3 μm~0.1 μm of micro-filtrate membrane filtration system refers to the rejection of microfiltration membranes
Can be 3 μm~0.1 μm, such as：Molecular cut off is that 0.1 μm of microfiltration membrane system refers to that it can retain point more than 0.1 μm
Son, pass through the molecule no more than 0.1 μm.Microfiltration membranes permeate refers to can be by the liquid of microfiltration membrane system.
Further, micro-filtrate membrane filtration system is used in parallel by three films, filter membrane be organic tubular type, doughnut or
Ceramic micro filter film forms；The operation temperature filtered by microfiltration membrane system is 15~35 DEG C, operating pressure is 0.1~
0.5MPa。
C. microfiltration membranes permeate was carried out by the rolling ultrafiltration membrane purification system that molecular cut off is 50~200kDa
Filter, obtains milipore filter permeate.Molecular cut off is that 50~200kDa rolling ultrafiltration membrane purification system refers to rolling ultrafiltration membrane
The cutoff performance of purification system is 50~200kDa, such as：Molecular cut off is that 200kDa rolling ultrafiltration membrane purification system is
200kDa molecule can be more than with molecular cut off by referring to it, pass through molecule of the molecular weight no more than 200kDa.Milipore filter passes through
Liquid refers to the liquid by rolling ultrafiltration membrane purification system.
Further, milipore filter permeate is purified into system by the rolling ultrafiltration membrane that molecular cut off is 500~1000Da
System is filtered, and obtains milipore filter trapped fluid.Molecular cut off is that 500~1000Da rolling ultrafiltration membrane purification system refers to roll up
The cutoff performance of formula milipore filter purification system is 500~1000Da, such as：Molecular cut off is that 500Da rolling ultrafiltration membrane is pure
Change system refers to that it can be more than 500Da molecule with molecular cut off, passes through molecule of the molecular weight no more than 500Da.Ultrafiltration
Film trapped fluid refers to the liquid of rolling ultrafiltration membrane purification system retention.
Further, rolling ultrafiltration membrane purification system is used in parallel using two films, filter membrane be containing (PS) polysulfones or
(PFS) filter membrane of polyether sulfone materials.The operation temperature purified by rolling ultrafiltration membrane purification system is 15~35 DEG C, behaviour
It is 0.3~0.8MPa to make pressure.
D. milipore filter trapped fluid is carried out by the rolling high pressure reverse osmosis membrane system that molecular cut off is 100~150Da
Concentration, moisture and small molecular weight impurity, mainly part residual inorganic ion are removed, obtains concentrate, the solid content of concentrate
Content >=30%.Molecular cut off is that 100~150Da rolling high pressure reverse osmosis membrane system refers to rolling high pressure counter-infiltration membrane system
The cutoff performance of system is 100~150Da, such as：Molecular cut off is that 100Da rolling high pressure reverse osmosis membrane system refers to that it can
It is more than 100Da molecule with molecular cut off, passes through molecule of the molecular weight no more than 100Da.Concentrate refers to rolling high pressure
The liquid of reverse osmosis membrane system retention.
Further, rolling high pressure reverse osmosis membrane system is high pressure concentration film, specifically by polyamide compoiste material film
It is made.Rolling high pressure reverse osmosis membrane system concentration systems are used using four film cascades.By rolling high pressure reverse osmosis membrane system
The operation temperature concentrated is 15~35 DEG C, and operating pressure is 3.5~5.5MPa.
E. concentrate is dried, obtains bitter gourd polypeptide extract.
Further, drying means is vacuum freeze drying or low temperature spray drying, obtains polypeptide protein content and is not less than
25% bitter gourd polypeptide extract.
The preparation method of this bitter gourd polypeptide is prepared using multi-level membrane separation and purification technology has regulation blood glucose function
Natural balsam pear polypeptide protein, exempt enzymolysis and high temperature enzyme deactivation step, with short production cycle, it is low to isolate and purify thickening temperature, effectively
The natural activity and its high content of the bitter melon protein polypeptide of preparation are ensure that, bitter gourd polypeptide egg in obtained bitter gourd polypeptide extract
White content is at least above 25%, and the polypeptide protein in other non-balsam pear sources such as soybean protein polypeptide is free of in extract product.
The weight molecule amount of bitter gourd polypeptide albumen is 4.5k~68kDa, wherein with regulation blood glucose function, clip size closest to pancreas
The content accounting example of 5.5~7kDa of island element molecular weight bitter gourd polypeptide fragment is 18~25%.Therefore the bitter gourd polypeptide obtained
Extract has extraordinary regulating and controlling blood sugar metabolic effects, the especially binding ability of insulin receptor and hypoglycemic effect.
Ginseng extraction concentrat is planted, the content of ginsenoside contained therein is more than 10%.Ginsenoside is a kind of
Triterpene saponin componds, it is primarily present in Panax medicinal material.It is more preferable, the ginsenoside extraction in present embodiment
Thing is prepared by extracting American Ginseng.Main component in American Ginseng is ginsenoside, and it can pass through solvent extraction legal system
It is standby to obtain.The ginsenoside extract extracted from American Ginseng can reduce blood glucose, regulation insulin secretion, promote glycometabolism and
Fat metabolism, there is certain booster action to treatment diabetes.
Kudzu root extract, it is prepared by using the root of kudzu vine of solvent extraction method extraction ordinary food level, it is contained therein
Main component be flavone compound (such as Puerarin, Radix Puerariae flavone glycoside, isoflavone aglycone etc.), its content 50% with
On, it has expansion blood vessel, reduces blood pressure, improves the effect of microcirculation, can improve insulin sensitivity and with hypoglycemic
Effect.
Siberian solomonseal rhizome polysaccharide extract, it is prepared by using the sealwort of solvent extraction method extraction ordinary food level, wherein
The content of Siberian solomonseal rhizome polysaccharide is more than 15%.Research shows that Siberian solomonseal rhizome polysaccharide extract is too high to blood glucose caused by adrenaline to be had
Obvious inhibitory action, therefore, Siberian solomonseal rhizome polysaccharide extract is added in the compound lozenge of the bitter gourd polypeptide, can concertedness enhancing it is bitter
The hypoglycemic effect of melon polypeptide.
In the present embodiment, ginsenoside extract, kudzu root extract, Siberian solomonseal rhizome polysaccharide extract, can be from the market
Directly it is commercially available, or can be also prepared using solvent extraction method, solvent extraction method includes heating and refluxing extraction method, microwave
Shake extraction method, decocting method, infusion process, percolation etc..Extraction solvent is water or ethanol water mixed solution system.
Nutritious yeast, also make food female raw, be clinically usually used in treating indigestion.Nutritious yeast contains abundant B races dimension
Raw element, amino acid and trace element chromium.Chromium is known as activation to internal pancreas islet, contributes to insulin to promote glucose to enter thin
The efficiency of intracellular, it is blood sugar regulator, diabetes can be prevented.
Further, the compound lozenge of bitter gourd polypeptide also includes auxiliary material, and this kind of auxiliary material is food-grade, and auxiliary material includes filling
At least one of agent, adhesive, lubricant, disintegrant or flavouring.Wherein, filler includes but is not limited to：Pregelatinated forms sediment
At least one of powder, resistant dextrin, maltodextrin；Adhesive is selected from microcrystalline cellulose, HPMC, gelatin, list
At least one of syrup or carbomer；Lubricant includes but is not limited to：Magnesium stearate, aluminum stearate, talcum, polyethylene glycol,
D-sorbite；Disintegrant includes but is not limited to：Crosslinked carboxymethyl fecula sodium, Ac-Di-Sol and low-substituted hydroxypropyl
Any of base cellulose.Flavouring is selected from any of xylitol, menthol, sorbierite, orange syrup.
Present embodiment also provides a kind of preparation method of the compound lozenge of hypoglycemic bitter gourd polypeptide, and it includes：
Step S1：By the bitter gourd polypeptide extract, the plantation ginseng extraction concentrat, the kudzu root extract, institute
Rhizoma Polygonati extract and the nutritious yeast is stated to mix with carrier auxiliary materials such as filler, disintegrant, flavouring, adhesive or lubricants
Dried after even and cross 50 mesh sieves；
Step S2：It is by above-mentioned mixed powder through fast low temperature microwave sterilization or ultraviolet disinfection, mixed powder is straight according to specification
Connect compressing dry granulation.
The feature and performance of the present invention are described in further detail with reference to embodiments：
Embodiment 1
The present embodiment provides a kind of hypoglycemic compound lozenge of bitter gourd polypeptide, and the compound lozenge of the bitter gourd polypeptide includes：
Bitter gourd polypeptide extract 4Kg, plantation ginseng extraction concentrat 3Kg, Kudzu Flavonoids Extracts 2Kg, Siberian solomonseal rhizome polysaccharide carry
Take thing 2Kg, nutritious yeast 0.7 ‰.Wherein, in bitter gourd polypeptide extract bitter gourd polypeptide content be 28%, molecular weight be 5.5~
The content of 7kDa bitter gourd polypeptide is 22%；The content for planting ginsenoside in ginseng extract is 15%；In kudzu root extract
The content of general flavone is 60%；The content of Siberian solomonseal rhizome polysaccharide is 21% in Siberian solomonseal rhizome polysaccharide extract.
The preparation method of the compound lozenge of the bitter gourd polypeptide includes：
A. by bitter gourd polypeptide extract, plantation ginseng extraction concentrat, Kudzu Flavonoids Extracts, Siberian solomonseal rhizome polysaccharide extract with
And nutritious yeast with after the auxiliary materials and mixing such as 3% microcrystalline cellulose, 10% resistant dextrin, 3% magnesium stearate, 6% D-sorbite
Dry, sieving；
B. by above-mentioned mixed powder after fast low temperature microwave sterilization 30 minutes, by mixed powder according to the direct dry method pressure of specification
Piece.
Embodiment 2
The present embodiment provides a kind of Polypeptide-k buccal tablet with hypoglycemic effect, and the Polypeptide-k buccal tablet includes：
Bitter gourd polypeptide extract 3Kg, plantation ginseng extraction concentrat 4Kg, Kudzu Flavonoids Extracts 1Kg, Siberian solomonseal rhizome polysaccharide carry
Take thing 3Kg, nutritious yeast 0.6 ‰.Wherein, the content of bitter gourd polypeptide is 23% in bitter gourd polypeptide extract, and its middle-molecular-weihydroxyethyl is
The content of 5.5~7kDa bitter gourd polypeptide is 18%；The content for planting ginsenoside in ginseng extraction concentrat is 24%；The root of kudzu vine
The content of general flavone is 50% in extract；The content of Siberian solomonseal rhizome polysaccharide is 26% in Siberian solomonseal rhizome polysaccharide extract.
The preparation method of the compound lozenge of the bitter gourd polypeptide includes：
A. by bitter gourd polypeptide extract, plantation ginseng extraction concentrat, Kudzu Flavonoids Extracts, Siberian solomonseal rhizome polysaccharide extract with
And nutritious yeast and 4% microcrystalline cellulose, 6% resistant dextrin, 2% magnesium stearate, 6% hydroxypropyl cellulose, 0.1% menthol
Deng mix after dry, sieving；
B. by above-mentioned mixed powder after ultraviolet disinfection 45 minutes, by mixed powder according to the direct compressing dry granulation of specification.
Embodiment 3
The present embodiment provides a kind of Polypeptide-k buccal tablet with hypoglycemic effect, and the Polypeptide-k buccal tablet includes：
Bitter gourd polypeptide extract 5Kg, plantation ginseng extraction concentrat 2Kg, Kudzu Flavonoids Extracts 3Kg, Siberian solomonseal rhizome polysaccharide carry
Take thing 1Kg, nutritious yeast 0.8 ‰.Wherein, the content of bitter gourd polypeptide is 25% in bitter gourd polypeptide extract, and its middle-molecular-weihydroxyethyl is
The content of 5.5~7kDa bitter gourd polypeptide is 20%；The content for planting ginsenoside in ginseng extraction concentrat is 10%；The root of kudzu vine
The content of general flavone is 65% in extract；The content of Siberian solomonseal rhizome polysaccharide is 15% in Siberian solomonseal rhizome polysaccharide extract.
The preparation method of the compound lozenge of the bitter gourd polypeptide includes：
A. by bitter gourd polypeptide extract, plantation ginseng extraction concentrat, Kudzu Flavonoids Extracts, Siberian solomonseal rhizome polysaccharide extract with
And nutritious yeast and 1% microcrystalline cellulose, 6% resistant dextrin, 2% magnesium stearate, 10% hydroxypropyl cellulose, 1% xylitol
Deng mix after dry, sieving；
B. by above-mentioned mixed powder after fast low temperature microwave sterilization 45 minutes, by mixed powder according to the direct dry method pressure of specification
Piece.
Wherein, the preparation method of bitter gourd polypeptide extract includes：
Longer from growth period, the fresh bitter that green is partially yellow, balsam pear seed is fuller is first by balsam pear raw material as raw material
Clean through running water, then cleaned through electrolytic high-concentration ozone water.Measuring Ozone Concentration in Water 12mg/L, water-carrying capacity 200L/h are cleaned,
To reduce agriculture residual pesticide and microorganism residual.Balsam pear raw material pulping after cleaning was broken into the liquid material of 20 eye mesh screens.
By be beaten it is broken after balsam pear raw material pour into Ultrasonic Wave-Assisted Extraction tank, and 10 mass times, 50 DEG C of purified water are added, using slow
Salt regulation system pH ≈ 7 are rushed, the adverse current for carrying out ultrasonic wave added is extracted 3 times, every time 30 minutes.
Centrifuged, and removed slag using high speed tube centrifuge, obtain centrifugate.By molecular cut off it is 1 μ by centrifugate
M hollow fiber microfiltration membrane system is filtered, and operation temperature is 30 DEG C, operating pressure 0.3MPa, obtains microfiltration membranes permeate.
First microfiltration membranes permeate was carried out by the rolling ultrafiltration membrane purification system that molecular cut off is 200kDa respectively
Filter, operation temperature are 30 DEG C, operating pressure 0.8MPa, obtain milipore filter permeate；Will transmit through liquid again is by molecular cut off
800Da rolling ultrafiltration membrane purification system is filtered, and operation temperature is 30 DEG C, pressure 0.8MPa, obtains milipore filter retention
Liquid.
Milipore filter trapped fluid is concentrated by the rolling high pressure reverse osmosis membrane system that molecular cut off is 150Da, grasped
Make temperature as 30 DEG C, operating pressure 4MPa, obtain the concentrate of solid content >=30%.Using the side of vacuum freeze drying
Concentrate is dried formula, obtains bitter gourd polypeptide extract, and the content of its polypeptide protein is up to 28.5%.
The polypeptide protein in obtained bitter gourd polypeptide extract is detected using gel electrophoresis, as shown in figure 1,
Fig. 1 is the SDS-PAGE of the polypeptide protein molecular weight distribution of bitter gourd polypeptide extract in the embodiment of the present invention；Can by Fig. 1
Know：In the bitter gourd polypeptide extract obtained by preparation method provided according to the present embodiment, molecular weight is 6kDa or so balsam pear
The content accounting example of polypeptide is more than 22%, and its clip size is close to insulin molecule amount, the binding ability with insulin receptor
By force, it can effectively activate insulin receptor protein signal path and recover damaged islet cells function.Therefore, according to the present embodiment
The obtained bitter gourd polypeptide extract of technical matters in contain a large amount of natural activity bitter gourd polypeptide eggs with regulation blood glucose function
In vain.
The hypoglycemic activity of the compound lozenge of hypoglycemic bitter gourd polypeptide provided below embodiment 1~3 carries out evaluation test：
First, experimental method
1. modeling：The SD male rats 120,150 ± 20g of body weight of healthy adult are chosen, are randomly divided into 6 groups, every group 20
Only.Wherein 5 groups after 24 hours, are injected intraperitoneally streptozocin (dosage 45mg/kg), and after injecting 72h, water 6h, tail are can't help in fasting
Venous blood sampling, survey blood glucose；Take blood glucose value >=16.8mM rat to continue modeling, raise with high lipid food；Blank group is in equal conditions
The lower isodose physiological saline of intraperitoneal injection, is raised with normal diet.After four weeks, tail vein takes blood to survey blood glucose, and Metabolic cage method surveys 24h
Urine protein content, fasting blood-glucose (BG) value >=16.8mM, while 24h quantity of proteinuria >=20mg/24h, judge diabetes rat
Modeling success.
2. administration：Rat after modeling success is randomly divided into 5 groups, respectively model control group, positive controls, medicine
High dose group, medicine middle dose group and medicine low dose group.After Polypeptide-k buccal tablet grinding in embodiment 1 pure water will be added to match somebody with somebody
Gavage decoction is made, the same day prepares, respectively by low dose group (dosage 150mg/kg), middle dose group (dosage 200mg/
Kg), high dose group (dosage 400mg/kg) gavage；To the rat oral gavage Metformin hydrochloride of positive controls, dosage
200mg/kg；Model control group and blank group under equal conditions gavage physiological saline.Every group of daily gavage once, successive administration
30 days.
3. detection：After last dose 2 hours, tail vein takes blood, surveys rat blood sugar content and saccharification hemoglobin content,
As a result it is as shown in table 1.
2nd, experimental result：
Table 1：Influence of the Polypeptide-k buccal tablet various dose group to rat blood sugar in embodiment 1
Note：Compared with blank control group,**p<0.01；Compared with model group,#p<0.05,##p<0.01。
As shown in Table 1, compared to blank control group, the fasting blood-glucose of model group and the content of glycosylated hemoglobin are significantly
Rise, illustrate diabetes rat modeling success.Compared with model group, positive controls and administration group (high, medium and low dosage group)
Fasting blood-glucose and the content of glycosylated hemoglobin have declined, and significant difference is presented, wherein, declined with medicine high dose group
Become apparent, show that the compound lozenge of the bitter gourd polypeptide has preferable therapeutic effect to diabetes, and therapeutic effect is in dose-dependant
Property.
In addition, the compound lozenge of bitter gourd polypeptide that embodiment 2 and embodiment 3 provide is used to carry out above-mentioned experiment, as a result with
Embodiment 1 is similar, therefore does not list one by one.
In summary, a kind of hypoglycemic compound lozenge of bitter gourd polypeptide provided by the invention and preparation method thereof, based on balsam pear
Hypoglycemic modern medicine study and the traditional Chinese medical science diabetes of diabetes are theoretical, reasonable recipe.High efficiency extraction, separation, purifying first
A large amount of to have the specified molecular weight for stablizing regulating and controlling blood sugar metabolic function and the natural activity bitter gourd polypeptide albumen of sequence, content exists
More than 25%, the content accounting example for the bitter gourd polypeptide fragment that its middle-molecular-weihydroxyethyl is 5.5~7kDa is 18~25%, its insulin by
Body binding ability is extremely strong, can activate islet receptor protein signal path and recover damaged islet cells function, and then can be effective
Blood sugar recovery is adjusted and controlled to normal level.In addition combined with traditional Chinese medicine unique formula from American Ginseng, the root of kudzu vine, the Radix Astragali, and
Addition can strengthen the nutritious yeast of the trace element containing chromium etc. of human insulin sensitiveness through being combined.Drop provided by the invention
The technical matters of the compound lozenge of blood glucose bitter gourd polypeptide is stable, and quality controllability is strong, ensure that product nucleus stability and
Conspicuousness, and it is adapted to industrialized production.The compound lozenge of bitter gourd polypeptide being prepared has that blood sugar decreasing effect is notable, stable effect
And the advantages of convenient to take, it can be effectively used for diabetes and its metabolism of blood glucose regulation and control of patient's early stage.
Although illustrate and describing the present invention with specific embodiment, but will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of compound lozenge of hypoglycemic bitter gourd polypeptide, it is characterised in that the compound lozenge of bitter gourd polypeptide includes：
The bitter gourd polypeptide extract of 3~5 parts by weight, in the bitter gourd polypeptide extract content of bitter gourd polypeptide albumen 20% with
On；
The ginseng extraction concentrat of 2~4 parts by weight, in the ginseng extraction concentrat content of general ginsenoside 10% with
On；
The kudzu root extract of 1~3 parts by weight, the content of Pueraria Flavonid is more than 50% in the kudzu root extract；
The Rhizoma Polygonati extract of 1~3 parts by weight, the content of Siberian solomonseal rhizome polysaccharide is more than 30% in the Rhizoma Polygonati extract；And
Nutritious yeast 0.6~0.8 ‰.
2. the compound lozenge of hypoglycemic bitter gourd polypeptide according to claim 1, it is characterised in that the bitter gourd polypeptide extract
For the content of middle bitter gourd polypeptide albumen more than 20%, its middle-molecular-weihydroxyethyl is the content accounting of 5.5~7kDa bitter gourd polypeptide fragment
Example is 18~25%.
3. the compound lozenge of hypoglycemic bitter gourd polypeptide according to claim 1, it is characterised in that the ginseng extraction concentrat
It is by extracting artificial growth 5 years and following ginseng and being prepared through 5~10 times of concentrations.
4. the compound lozenge of hypoglycemic bitter gourd polypeptide according to claim 1, it is characterised in that the bitter gourd polypeptide is compound to be contained
Piece also includes the auxiliary material of food-grade, and the auxiliary material is included in filler, adhesive, lubricant, disintegrant or flavouring at least
It is one or more of.
5. the compound lozenge of hypoglycemic bitter gourd polypeptide according to claim 4, it is characterised in that the flavouring is selected from
Any of xylitol, menthol, D-sorbite or orange syrup.
6. the compound lozenge of hypoglycemic bitter gourd polypeptide according to claim 4, it is characterised in that described adhesive is selected from crystallite
At least one of cellulose, hydroxypropyl cellulose, gelatin, simple syrup or carbomer.
7. the compound lozenge of hypoglycemic bitter gourd polypeptide according to claim 1 and claim 2, it is characterised in that the hardship
The preparation method of melon polypeptide extract includes：
The cleaning of balsam pear raw material, mashing is broken, with purification flooding balsam pear raw material, then centrifuge and remove slag, obtain centrifugate；
The centrifugate is filtered by the microfiltration membrane system that molecular cut off is 3 μm~0.1 μm, it is miscellaneous to remove macromolecular
Matter, obtain microfiltration membranes permeate；
The microfiltration membranes permeate is filtered by the rolling ultrafiltration membrane purification system that molecular cut off is 150kDa, obtained super
Filter membrane permeate；The milipore filter permeate was carried out through the rolling ultrafiltration membrane purification system that molecular cut off is 800Da again
Filter, obtains milipore filter trapped fluid；
The trapped fluid is concentrated by molecular cut off >=100Da rolling high pressure reverse osmosis membrane system, removes moisture
And part residual inorganic salts and small molecular weight impurity, obtain concentrate, solid content >=30% of the concentrate；
The concentrate is dried, obtains the bitter gourd polypeptide extract powder that polypeptide protein content is not less than 20%.
8. the compound lozenge of bitter gourd polypeptide according to claim 7, it is characterised in that the rolling ultrafiltration membrane purification system is adopted
Used in parallel with two films, filter membrane is the filter membrane containing polysulfones or polyether sulfone materials.Carried out by roll-to-roll ultrafiltration membranous system
The operation temperature of purifying is 15~35 DEG C, and operating pressure is 0.3~0.8MPa.
9. Polypeptide-k buccal tablet according to claim 7, it is characterised in that the rolling high pressure reverse osmosis membrane concentration system
Used using four film cascades, the operation temperature concentrated by rolling high pressure reverse osmosis membrane is 15~35 DEG C, operating pressure
For 3.5~5.5MPa.
10. a kind of preparation method of compound lozenge of hypoglycemic bitter gourd polypeptide as described in any one of claim 1~9, its feature
It is, it includes：By the bitter gourd polypeptide extract, the plantation ginseng extraction concentrat, the kudzu root extract, the Huang
After essence extract and the carrier auxiliary material such as the nutritious yeast and filler, disintegrant, flavouring, adhesive or lubricant mix
Dry and cross 50 mesh sieves, mixed powder is obtained after fast low temperature microwave sterilization or ultraviolet disinfection；Mixed powder is direct according to specification
Compressing dry granulation.
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| CN110801024A (en) * | 2019-10-25 | 2020-02-18 | 运鸿集团股份有限公司 | Polysaccharide composite polypeptide for reducing blood sugar, blood fat and glycosylated hemoglobin and preparation method thereof |
| CN110800994A (en) * | 2019-10-25 | 2020-02-18 | 武汉轻工大学 | Compound nutrient for reducing values of blood sugar, urine sugar and glycosylated hemoglobin and preparation method thereof |
| CN114146183A (en) * | 2021-12-02 | 2022-03-08 | 武汉轻工大学 | Purple sweet potato blood sugar-reducing polypeptide microencapsulated product and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110800994A (en) * | 2019-10-25 | 2020-02-18 | 武汉轻工大学 | Compound nutrient for reducing values of blood sugar, urine sugar and glycosylated hemoglobin and preparation method thereof |
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| CN114146183A (en) * | 2021-12-02 | 2022-03-08 | 武汉轻工大学 | Purple sweet potato blood sugar-reducing polypeptide microencapsulated product and preparation method and application thereof |
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