CN107429292B - 底数大于2的指数核酸扩增 - Google Patents

底数大于2的指数核酸扩增 Download PDF

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CN107429292B
CN107429292B CN201580076131.0A CN201580076131A CN107429292B CN 107429292 B CN107429292 B CN 107429292B CN 201580076131 A CN201580076131 A CN 201580076131A CN 107429292 B CN107429292 B CN 107429292B
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primer
sequence
stranded
strand
double
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CN107429292A (zh
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R·希古奇
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Cepheid
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201580076131.0A 2014-12-15 2015-12-15 底数大于2的指数核酸扩增 Active CN107429292B (zh)

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US201462092102P 2014-12-15 2014-12-15
US62/092,102 2014-12-15
PCT/US2015/065890 WO2016100388A1 (en) 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification

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US (3) US10273534B2 (OSRAM)
EP (1) EP3234188B1 (OSRAM)
JP (1) JP6804462B2 (OSRAM)
CN (2) CN114369596B (OSRAM)
AU (2) AU2015362617B2 (OSRAM)
CA (1) CA2971006C (OSRAM)
DK (1) DK3234188T3 (OSRAM)
ES (1) ES2764975T3 (OSRAM)
HK (1) HK1247255A1 (OSRAM)
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JP6804462B2 (ja) 2014-12-15 2020-12-23 セファイド 指数関数の底が２より大きい核酸増幅
EP3287528A1 (de) * 2016-08-25 2018-02-28 AGCT GmbH Verfahren zur amplifikation von nukleinsäuren und kit zu dessen durchführung
US20210071243A1 (en) * 2018-02-26 2021-03-11 Agct Gmbh Method for primer extension reaction with improved specificity
WO2019162530A1 (de) * 2018-02-26 2019-08-29 Agct Gmbh Agverfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
EP3759246A1 (de) * 2018-02-28 2021-01-06 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
AU2019371797A1 (en) * 2018-10-29 2021-05-20 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
US20210403981A1 (en) * 2020-04-27 2021-12-30 Cepheid Exponential base-3 and greater nucleic acid amplification with cycling probe
US20230250471A1 (en) * 2020-06-23 2023-08-10 HeimBiotek, Inc. Composition for sequential polymerase chain reaction, and gene amplification method using same
AU2021409723A1 (en) 2020-12-24 2023-06-22 Integrated Dna Technologies, Inc. Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases
WO2023158644A1 (en) * 2022-02-15 2023-08-24 Cepheid Exponential base-greater-than-base 2 nucleic acid amplification using high- and low-tm primers
US20240336961A1 (en) * 2023-02-23 2024-10-10 Cepheid USE OF DESTABILIZING NUCLEOTIDE TRIPHOSPHATES TO REDUCE AMPLICON FOLDING AND Tm

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CA2971006C (en) 2024-05-21
HK1247255A1 (zh) 2018-09-21
US20210395797A1 (en) 2021-12-23
PT3234188T (pt) 2020-01-16
DK3234188T3 (da) 2020-01-20
CN114369596A (zh) 2022-04-19
US20180066304A1 (en) 2018-03-08
ES2764975T3 (es) 2020-06-05
CN114369596B (zh) 2025-07-08
EP3234188B1 (en) 2019-10-23
AU2022203553A1 (en) 2022-06-16
AU2015362617B2 (en) 2022-03-03
JP6804462B2 (ja) 2020-12-23
CN107429292A (zh) 2017-12-01
CA2971006A1 (en) 2016-06-23
EP3234188A1 (en) 2017-10-25
JP2017537658A (ja) 2017-12-21
WO2016100388A1 (en) 2016-06-23
US11952621B2 (en) 2024-04-09
US11028434B2 (en) 2021-06-08
US20190203266A1 (en) 2019-07-04
AU2022203553B2 (en) 2024-08-08
US10273534B2 (en) 2019-04-30
AU2015362617A1 (en) 2017-06-29

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