CN107428795B - 用于标记dna编码文库的方法 - Google Patents

用于标记dna编码文库的方法 Download PDF

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Publication number
CN107428795B
CN107428795B CN201580077171.7A CN201580077171A CN107428795B CN 107428795 B CN107428795 B CN 107428795B CN 201580077171 A CN201580077171 A CN 201580077171A CN 107428795 B CN107428795 B CN 107428795B
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nucleotides
oligonucleotide
chemical
headpiece
library
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CN201580077171.7A
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English (en)
Chinese (zh)
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CN107428795A (zh
Inventor
A.D.基夫
A.利托夫基克
M.克拉克
R.W.沃纳
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X Chem Inc
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X Chem Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Structural Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
CN201580077171.7A 2014-12-30 2015-12-28 用于标记dna编码文库的方法 Expired - Fee Related CN107428795B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462098037P 2014-12-30 2014-12-30
US62/098037 2014-12-30
PCT/US2015/067667 WO2016109423A1 (en) 2014-12-30 2015-12-28 Methods for tagging dna-encoded libraries

Publications (2)

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CN107428795A CN107428795A (zh) 2017-12-01
CN107428795B true CN107428795B (zh) 2021-06-18

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Country Link
US (1) US20180002688A1 (https=)
EP (1) EP3240795A4 (https=)
JP (1) JP6864621B2 (https=)
KR (1) KR20170094445A (https=)
CN (1) CN107428795B (https=)
AU (2) AU2015374309B2 (https=)
BR (1) BR112017013904A2 (https=)
CA (1) CA2972275A1 (https=)
EA (1) EA201791312A1 (https=)
HK (1) HK1246301A1 (https=)
IL (2) IL253154B (https=)
MA (1) MA41298A (https=)
MX (1) MX2017008772A (https=)
SG (1) SG11201705350XA (https=)
WO (1) WO2016109423A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11186836B2 (en) 2016-06-16 2021-11-30 Haystack Sciences Corporation Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
EA201992476A1 (ru) * 2017-04-18 2020-02-25 Икс-Чем, Инк. Способы идентификации соединений
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation OLIGONUCLEOTID-DRIVEN COMBINATORY SYNTHESIS VERIFICATION MOLECULES AND ASSOCIATED MANUFACTURING AND USE PROCESSES
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
WO2020001560A1 (zh) * 2018-06-29 2020-01-02 成都先导药物开发股份有限公司 一种合成dna编码化合物中的反应监测方法
CN109468310B (zh) * 2018-10-25 2020-12-01 深圳劲宇生物科技有限公司 Dna编码碎片分子库的合成方法和连接基团的筛选方法
CA3131890A1 (en) * 2019-03-14 2020-09-17 Haystack Sciences Corporation Methods and systems for processing or analyzing oligonucleotide encoded molecules
CN112941634B (zh) * 2019-12-10 2023-09-26 成都先导药物开发股份有限公司 通过dna编码化合物库筛选同时结合多个生物靶标的化合物的方法
AU2021376394A1 (en) * 2020-11-06 2023-06-15 The University Of Chicago Hairpin oligonucleotides and uses thereof
WO2025053257A1 (ja) * 2023-09-07 2025-03-13 旭化成ファーマ株式会社 オリゴヌクレオチドによりコード化された化合物の製造方法、およびその応用

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
CN103998658A (zh) * 2011-09-07 2014-08-20 X-化学有限公司 用于标记dna-编码文库的方法

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
WO1996038726A1 (en) * 1995-05-30 1996-12-05 Ecole Polytechnique Federale De Lausanne (Epfl) Covalently immobilized phospholipid bilayers on solid surfaces
PT2348125T (pt) * 2002-10-30 2017-08-29 Nuevolution As Método para a síntese de um complexo bifuncional
US8846883B2 (en) * 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
EA201992285A1 (ru) * 2012-07-13 2020-05-31 Икс-Чем, Инк. Днк-кодированные библиотеки, содержащие связи кодирующих олигонуклеотидов, не доступные для считывания полимеразами

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
CN1898257A (zh) * 2003-12-17 2007-01-17 普雷西斯药品公司 合成编码文库的方法
CN103998658A (zh) * 2011-09-07 2014-08-20 X-化学有限公司 用于标记dna-编码文库的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nonenzymatic ligation of double-helical DNA by alternate-strand triple helix formation;Kevin J.Luebke等;《Nucleic Acids Research》;19920515;第20卷(第12期);第3005-3009页 *
Template-Directed Polymerization of Oligoadenylates Using Cyanogen Bromide;Eiko Kanaya等;《Biochemistry》;19861118;第25卷(第23期);第7423 -7430页 *

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Publication number Publication date
AU2015374309B2 (en) 2020-06-11
AU2015374309A1 (en) 2017-07-27
EA201791312A1 (ru) 2017-10-31
MX2017008772A (es) 2018-03-15
JP2018501796A (ja) 2018-01-25
IL253154B (en) 2020-10-29
CN107428795A (zh) 2017-12-01
IL278117A (en) 2020-11-30
WO2016109423A1 (en) 2016-07-07
HK1246301A1 (zh) 2018-09-07
EP3240795A4 (en) 2019-02-13
BR112017013904A2 (pt) 2018-01-02
JP6864621B2 (ja) 2021-04-28
IL253154A0 (en) 2017-08-31
US20180002688A1 (en) 2018-01-04
SG11201705350XA (en) 2017-08-30
EP3240795A1 (en) 2017-11-08
MA41298A (fr) 2017-11-07
CA2972275A1 (en) 2016-07-07
AU2020230345A1 (en) 2020-10-01
KR20170094445A (ko) 2017-08-17
WO2016109423A8 (en) 2017-07-20

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Granted publication date: 20210618