MX2017008772A - Metodos para etiquetar bibliotecas codificadas por adn. - Google Patents

Metodos para etiquetar bibliotecas codificadas por adn.

Info

Publication number
MX2017008772A
MX2017008772A MX2017008772A MX2017008772A MX2017008772A MX 2017008772 A MX2017008772 A MX 2017008772A MX 2017008772 A MX2017008772 A MX 2017008772A MX 2017008772 A MX2017008772 A MX 2017008772A MX 2017008772 A MX2017008772 A MX 2017008772A
Authority
MX
Mexico
Prior art keywords
methods
monophospho
termini
hydroxy
wild
Prior art date
Application number
MX2017008772A
Other languages
English (en)
Inventor
w wagner Richard
Litovchick Alexander
Clark Matthew
D Keefe Anthony
Original Assignee
X Chem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by X Chem Inc filed Critical X Chem Inc
Publication of MX2017008772A publication Critical patent/MX2017008772A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Structural Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a métodos para producir entidades químicas codificadas. En particular, los oligonucleótidos y métodos pueden incluir entidades químicas codificadas teniendo eslabones tipo silvestre formados a través de técnicas de ligación química. Una estrategia que puede utilizarse que aprovecha simultáneamente la ligación química como medio para codificar la historia química, mientras también conserva la capacidad de las polimerasas a recuperar directamente secuencia de marbete e información de asociación, es la de llevar a cabo ligación química en una manera que genere eslabones fosfodiéster tipo silvestre. Tales métodos utilizan generalmente agentes de condensación tales como bromuro de cianógeno o similares junto con 5´-fosfato y 3´-hidroxil oligonucleótidos en una contexto de doble hélice o de plantilla. De manera similar, el bromuro de cianógeno también ha mostrado ligar químicamente pares de oligonucleótidos de sustrato que son 5´-hidroxil y 3´-fosfato. Sin embargo, estos métodos sufren de una pobre eficiencia que los hace no adecuados para uso en un proceso iterativo tal como marbetar bibliotecas codificadas en ADN.
MX2017008772A 2014-12-30 2015-12-28 Metodos para etiquetar bibliotecas codificadas por adn. MX2017008772A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462098037P 2014-12-30 2014-12-30
PCT/US2015/067667 WO2016109423A1 (en) 2014-12-30 2015-12-28 Methods for tagging dna-encoded libraries

Publications (1)

Publication Number Publication Date
MX2017008772A true MX2017008772A (es) 2018-03-15

Family

ID=56284965

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017008772A MX2017008772A (es) 2014-12-30 2015-12-28 Metodos para etiquetar bibliotecas codificadas por adn.

Country Status (15)

Country Link
US (1) US20180002688A1 (es)
EP (1) EP3240795A4 (es)
JP (1) JP6864621B2 (es)
KR (1) KR20170094445A (es)
CN (1) CN107428795B (es)
AU (2) AU2015374309B2 (es)
BR (1) BR112017013904A2 (es)
CA (1) CA2972275A1 (es)
EA (1) EA201791312A1 (es)
HK (1) HK1246301A1 (es)
IL (2) IL253154B (es)
MA (1) MA41298A (es)
MX (1) MX2017008772A (es)
SG (1) SG11201705350XA (es)
WO (1) WO2016109423A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109312492B (zh) 2016-06-16 2022-10-04 哈斯达克科学公司 寡核苷酸指导和记录的编码探针分子的组合合成
EA201992476A1 (ru) * 2017-04-18 2020-02-25 Икс-Чем, Инк. Способы идентификации соединений
WO2018204420A1 (en) 2017-05-02 2018-11-08 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
WO2020001560A1 (zh) * 2018-06-29 2020-01-02 成都先导药物开发股份有限公司 一种合成dna编码化合物中的反应监测方法
CN109468310B (zh) * 2018-10-25 2020-12-01 深圳劲宇生物科技有限公司 Dna编码碎片分子库的合成方法和连接基团的筛选方法
US20220145362A1 (en) * 2019-03-14 2022-05-12 Haystack Sciences Corporation Methods and systems for processing or analyzing oligonucleotide encoded molecules
WO2021115319A1 (zh) * 2019-12-10 2021-06-17 成都先导药物开发股份有限公司 同时结合多个生物靶标的dna编码化合物库筛选方法
JP2023548857A (ja) * 2020-11-06 2023-11-21 ザ ユニヴァーシティー オヴ シカゴ ヘアピンオリゴヌクレオチド及びその使用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996038726A1 (en) * 1995-05-30 1996-12-05 Ecole Polytechnique Federale De Lausanne (Epfl) Covalently immobilized phospholipid bilayers on solid surfaces
CN106337046B (zh) * 2002-10-30 2021-03-23 纽韦卢森公司 合成双功能复合物的方法
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US8846883B2 (en) * 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
EP2748357B1 (en) * 2011-09-07 2018-04-04 X-Chem, Inc. Methods for tagging dna-encoded libraries
KR102146721B1 (ko) * 2012-07-13 2020-08-21 엑스-켐, 인크. 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리

Also Published As

Publication number Publication date
CN107428795A (zh) 2017-12-01
IL253154B (en) 2020-10-29
JP6864621B2 (ja) 2021-04-28
IL278117A (en) 2020-11-30
WO2016109423A1 (en) 2016-07-07
BR112017013904A2 (pt) 2018-01-02
JP2018501796A (ja) 2018-01-25
WO2016109423A8 (en) 2017-07-20
KR20170094445A (ko) 2017-08-17
IL253154A0 (en) 2017-08-31
MA41298A (fr) 2017-11-07
AU2015374309A1 (en) 2017-07-27
AU2020230345A1 (en) 2020-10-01
AU2015374309B2 (en) 2020-06-11
CA2972275A1 (en) 2016-07-07
EP3240795A4 (en) 2019-02-13
HK1246301A1 (zh) 2018-09-07
EA201791312A1 (ru) 2017-10-31
US20180002688A1 (en) 2018-01-04
SG11201705350XA (en) 2017-08-30
CN107428795B (zh) 2021-06-18
EP3240795A1 (en) 2017-11-08

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