CN107428795B - 用于标记dna编码文库的方法 - Google Patents

用于标记dna编码文库的方法 Download PDF

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Publication number
CN107428795B
CN107428795B CN201580077171.7A CN201580077171A CN107428795B CN 107428795 B CN107428795 B CN 107428795B CN 201580077171 A CN201580077171 A CN 201580077171A CN 107428795 B CN107428795 B CN 107428795B
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nucleotides
oligonucleotide
chemical
headpiece
library
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Expired - Fee Related
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CN201580077171.7A
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English (en)
Chinese (zh)
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CN107428795A (zh
Inventor
A.D.基夫
A.利托夫基克
M.克拉克
R.W.沃纳
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X Chem Inc
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X Chem Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580077171.7A 2014-12-30 2015-12-28 用于标记dna编码文库的方法 Expired - Fee Related CN107428795B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462098037P 2014-12-30 2014-12-30
US62/098037 2014-12-30
PCT/US2015/067667 WO2016109423A1 (en) 2014-12-30 2015-12-28 Methods for tagging dna-encoded libraries

Publications (2)

Publication Number Publication Date
CN107428795A CN107428795A (zh) 2017-12-01
CN107428795B true CN107428795B (zh) 2021-06-18

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CN201580077171.7A Expired - Fee Related CN107428795B (zh) 2014-12-30 2015-12-28 用于标记dna编码文库的方法

Country Status (15)

Country Link
US (1) US20180002688A1 (es)
EP (1) EP3240795A4 (es)
JP (1) JP6864621B2 (es)
KR (1) KR20170094445A (es)
CN (1) CN107428795B (es)
AU (2) AU2015374309B2 (es)
BR (1) BR112017013904A2 (es)
CA (1) CA2972275A1 (es)
EA (1) EA201791312A1 (es)
HK (1) HK1246301A1 (es)
IL (2) IL253154B (es)
MA (1) MA41298A (es)
MX (1) MX2017008772A (es)
SG (1) SG11201705350XA (es)
WO (1) WO2016109423A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3472376A4 (en) 2016-06-16 2019-12-18 Richard Edward Watts DIRECTED AND RECORDED COMBINATORY SYNTHESIS OF OLIGONUCLEOTIDES OF CODED PROBE MOLECULES
CN110730822B (zh) * 2017-04-18 2024-03-08 X-化学有限公司 鉴定化合物的方法
WO2018204420A1 (en) 2017-05-02 2018-11-08 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
CN110658163A (zh) * 2018-06-29 2020-01-07 成都先导药物开发股份有限公司 一种合成dna编码化合物中的反应监测方法
CN109468310B (zh) * 2018-10-25 2020-12-01 深圳劲宇生物科技有限公司 Dna编码碎片分子库的合成方法和连接基团的筛选方法
CN113677836A (zh) * 2019-03-14 2021-11-19 海斯塔克科学公司 用于处理或分析寡核苷酸编码分子的方法和系统
CN112941634B (zh) * 2019-12-10 2023-09-26 成都先导药物开发股份有限公司 通过dna编码化合物库筛选同时结合多个生物靶标的化合物的方法
AU2021376394A1 (en) * 2020-11-06 2023-06-15 The University Of Chicago Hairpin oligonucleotides and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
CN103998658A (zh) * 2011-09-07 2014-08-20 X-化学有限公司 用于标记dna-编码文库的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996038726A1 (en) * 1995-05-30 1996-12-05 Ecole Polytechnique Federale De Lausanne (Epfl) Covalently immobilized phospholipid bilayers on solid surfaces
ES2640279T3 (es) * 2002-10-30 2017-11-02 Nuevolution A/S Procedimiento para la síntesis de un complejo bifuncional
US8846883B2 (en) * 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
IL236633B (en) * 2012-07-13 2022-07-01 X Chem Inc DNA-encoded libraries containing a conjugate containing conjugated oligonucleotide links

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
CN1898257A (zh) * 2003-12-17 2007-01-17 普雷西斯药品公司 合成编码文库的方法
CN103998658A (zh) * 2011-09-07 2014-08-20 X-化学有限公司 用于标记dna-编码文库的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nonenzymatic ligation of double-helical DNA by alternate-strand triple helix formation;Kevin J.Luebke等;《Nucleic Acids Research》;19920515;第20卷(第12期);第3005-3009页 *
Template-Directed Polymerization of Oligoadenylates Using Cyanogen Bromide;Eiko Kanaya等;《Biochemistry》;19861118;第25卷(第23期);第7423 -7430页 *

Also Published As

Publication number Publication date
JP6864621B2 (ja) 2021-04-28
HK1246301A1 (zh) 2018-09-07
EP3240795A4 (en) 2019-02-13
AU2020230345A1 (en) 2020-10-01
CN107428795A (zh) 2017-12-01
KR20170094445A (ko) 2017-08-17
MA41298A (fr) 2017-11-07
EP3240795A1 (en) 2017-11-08
CA2972275A1 (en) 2016-07-07
MX2017008772A (es) 2018-03-15
AU2015374309A1 (en) 2017-07-27
SG11201705350XA (en) 2017-08-30
WO2016109423A8 (en) 2017-07-20
IL253154B (en) 2020-10-29
JP2018501796A (ja) 2018-01-25
IL278117A (en) 2020-11-30
BR112017013904A2 (pt) 2018-01-02
EA201791312A1 (ru) 2017-10-31
AU2015374309B2 (en) 2020-06-11
IL253154A0 (en) 2017-08-31
US20180002688A1 (en) 2018-01-04
WO2016109423A1 (en) 2016-07-07

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