CN107428795B - 用于标记dna编码文库的方法 - Google Patents

用于标记dna编码文库的方法 Download PDF

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Publication number
CN107428795B
CN107428795B CN201580077171.7A CN201580077171A CN107428795B CN 107428795 B CN107428795 B CN 107428795B CN 201580077171 A CN201580077171 A CN 201580077171A CN 107428795 B CN107428795 B CN 107428795B
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nucleotides
oligonucleotide
chemical
headpiece
library
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English (en)
Chinese (zh)
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CN107428795A (zh
Inventor
A.D.基夫
A.利托夫基克
M.克拉克
R.W.沃纳
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X Chem Inc
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X Chem Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Structural Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
CN201580077171.7A 2014-12-30 2015-12-28 用于标记dna编码文库的方法 Expired - Fee Related CN107428795B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462098037P 2014-12-30 2014-12-30
US62/098037 2014-12-30
PCT/US2015/067667 WO2016109423A1 (en) 2014-12-30 2015-12-28 Methods for tagging dna-encoded libraries

Publications (2)

Publication Number Publication Date
CN107428795A CN107428795A (zh) 2017-12-01
CN107428795B true CN107428795B (zh) 2021-06-18

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US (1) US20180002688A1 (enExample)
EP (1) EP3240795A4 (enExample)
JP (1) JP6864621B2 (enExample)
KR (1) KR20170094445A (enExample)
CN (1) CN107428795B (enExample)
AU (2) AU2015374309B2 (enExample)
BR (1) BR112017013904A2 (enExample)
CA (1) CA2972275A1 (enExample)
EA (1) EA201791312A1 (enExample)
HK (1) HK1246301A1 (enExample)
IL (2) IL253154B (enExample)
MA (1) MA41298A (enExample)
MX (1) MX2017008772A (enExample)
SG (1) SG11201705350XA (enExample)
WO (1) WO2016109423A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017218293A1 (en) 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
WO2018195134A1 (en) * 2017-04-18 2018-10-25 X-Chem, Inc. Methods for identifying compounds
WO2018204420A1 (en) 2017-05-02 2018-11-08 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
CN110658163A (zh) * 2018-06-29 2020-01-07 成都先导药物开发股份有限公司 一种合成dna编码化合物中的反应监测方法
CN109468310B (zh) * 2018-10-25 2020-12-01 深圳劲宇生物科技有限公司 Dna编码碎片分子库的合成方法和连接基团的筛选方法
JP7646212B2 (ja) * 2019-03-14 2025-03-17 ヘイスタック サイエンシーズ コーポレイション オリゴヌクレオチドコード化分子を加工または分析する方法および系
WO2021115319A1 (zh) * 2019-12-10 2021-06-17 成都先导药物开发股份有限公司 同时结合多个生物靶标的dna编码化合物库筛选方法
EP4240863A4 (en) * 2020-11-06 2025-03-12 The University of Chicago HAIRPIN OLIGONUCLEOTIDES AND THEIR USES
WO2025053257A1 (ja) * 2023-09-07 2025-03-13 旭化成ファーマ株式会社 オリゴヌクレオチドによりコード化された化合物の製造方法、およびその応用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
CN103998658A (zh) * 2011-09-07 2014-08-20 X-化学有限公司 用于标记dna-编码文库的方法

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
WO1996038726A1 (en) * 1995-05-30 1996-12-05 Ecole Polytechnique Federale De Lausanne (Epfl) Covalently immobilized phospholipid bilayers on solid surfaces
CN106337046B (zh) * 2002-10-30 2021-03-23 纽韦卢森公司 合成双功能复合物的方法
US8846883B2 (en) * 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
KR102146721B1 (ko) * 2012-07-13 2020-08-21 엑스-켐, 인크. 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058479A2 (en) * 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
CN1898257A (zh) * 2003-12-17 2007-01-17 普雷西斯药品公司 合成编码文库的方法
CN103998658A (zh) * 2011-09-07 2014-08-20 X-化学有限公司 用于标记dna-编码文库的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nonenzymatic ligation of double-helical DNA by alternate-strand triple helix formation;Kevin J.Luebke等;《Nucleic Acids Research》;19920515;第20卷(第12期);第3005-3009页 *
Template-Directed Polymerization of Oligoadenylates Using Cyanogen Bromide;Eiko Kanaya等;《Biochemistry》;19861118;第25卷(第23期);第7423 -7430页 *

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Publication number Publication date
EA201791312A1 (ru) 2017-10-31
EP3240795A1 (en) 2017-11-08
IL278117A (en) 2020-11-30
JP6864621B2 (ja) 2021-04-28
AU2015374309A1 (en) 2017-07-27
JP2018501796A (ja) 2018-01-25
CA2972275A1 (en) 2016-07-07
AU2015374309B2 (en) 2020-06-11
AU2020230345A1 (en) 2020-10-01
HK1246301A1 (zh) 2018-09-07
IL253154A0 (en) 2017-08-31
EP3240795A4 (en) 2019-02-13
MX2017008772A (es) 2018-03-15
US20180002688A1 (en) 2018-01-04
BR112017013904A2 (pt) 2018-01-02
SG11201705350XA (en) 2017-08-30
MA41298A (fr) 2017-11-07
KR20170094445A (ko) 2017-08-17
WO2016109423A1 (en) 2016-07-07
IL253154B (en) 2020-10-29
CN107428795A (zh) 2017-12-01
WO2016109423A8 (en) 2017-07-20

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