CN1074280C - Micro fetus active composite, its preparing process and application in cosmetics - Google Patents
Micro fetus active composite, its preparing process and application in cosmeticsInfo
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- CN1074280C CN1074280C CN96111452A CN96111452A CN1074280C CN 1074280 C CN1074280 C CN 1074280C CN 96111452 A CN96111452 A CN 96111452A CN 96111452 A CN96111452 A CN 96111452A CN 1074280 C CN1074280 C CN 1074280C
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- micro
- fetus
- active
- fetus active
- placenta hominis
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Abstract
The present invention relates to a micromolecular placentin compound, a preparation method thereof and an application thereof in cosmetics. The compound is mainly prepared by compounding placenta micromolecular placentin, fetal liver micromolecular placentin and mixed tissue micromolecular placentin. The preparation method of the micromolecular placentin compound is characterized in that the micromolecular placentin compound is obtained by being directly put into a dialysis bag filled with dialysis fluid and dialyzed after being homogenized. The compound can be used as a cosmetic for facial beautification. In the present invention, advanced biological product technologies are adopted from the preparation method, the product, packaging to storage, and therefore, the present invention guarantees the high purity and the high-efficiency biological activity of the product and the high safety of use.
Description
The present invention relates to a kind of bioactive substance and preparation method thereof and the application in cosmetics, particularly relate to micro fetus active composite and preparation method thereof and the application in cosmetics.
Nearly more than ten years, a kind of non-protide small molecule bioactive material that domestic and international many scholars extract from people's Placenta Hominis has very strong immunocompetence, and is named as placental immunity regulatory factor (PF).Discovering in recent years, PF has remarkable two-way immunomodulating, antiviral, antitumor and anti-aging effects, as viral hepatitis, poliomyelitis, dermopathic herpesvirus disease, malignant tumor, autoimmune disease and immunologic hypofunction disease thereof etc., obtained significant curative effect by the clinical various diseases of viral infection (particularly repeated infection) that are used for the treatment of.
In recent years, famous biotech specialist professor Wu Zuze of China finds that the small molecule bioactive material that extracts has significant antitumaous effect and immunoregulation effect from people embryonal tissue, and the low molecule tumour-inhibitory substance of called after [is seen: Wu Zuze.The excretory low molecule tumour-inhibitory substance of human foetus liver cell is to the effect of leukemia cell inhibiting.China's applied physiology magazine 1993; (1): 20-24].Chinese patent 93115199 also raiser body early embryo factor has very strong cancerocidal and immunological enhancement.But their research only limits to medical treatment and pharmacy aspect.
Has efficient non-protide small molecule bioactive material in the extract of the inventor with inspection method finder embryo who is similar to PF and Placenta Hominis, and with its called after micro fetus active (Micro-molecular Embryo Active Factor), be MEAF, contain profuse peptide class, nucleotide, various cell growth factor and multivitamin etc. among tire liver MEAF and the Placenta Hominis MEAF, but immunoregulatory factor is less relatively.Up to now, the report of relevant micro fetus active composite of Shang Weijian and similar complex.
The step of producing micro fetus active with conventional method is as follows: one, select healthy mature newborn baby's Placenta Hominis and tire liver and the heart that takes out from the people embryo, thymus, spleen and kidney to make raw material, require fresh totally, 30 ℃ of following room temperatures are placed and are no more than 8 hours.Two, the puerpera checks, liver function is normal, and HBsAg, HCAb, syphilis antibody and the inspection of HIV antibody all should be negative.Three, use the distilled water flushing Placenta Hominis, cut off fascia, weigh, shred, add equivalent sterile distilled water, with high-speed tissue mashing machine smash to pieces, homogenate.Four, the bag filter of packing into behind the homogenate low-temperature centrifugation is dialysed (dialysis solution is normal saline or sterile distilled water).The deficiency of this method is the equipment that the output of obtaining is on the low side and the low-temperature centrifugation need are expensive and has increased the chance of polluting.
In cosmetic field, the tradition skin protection cosmetics mostly is synthetic, often contain various chemical substances such as bleach hydroquinone, hydrargyrum, aluminum and other heavy metal, spice, hormone, vitamin etc., life-time service has significant cytotoxicity, makes skin aging ahead of time; Or contain macromole active substance (protein), animal and plant fat, vaseline etc., and be difficult for being absorbed by the epiderm skin cell, influence the breathing and the growth of cell, even cause various cosmetic dermatosises (as contact dermatitis, allergic dermatitis etc.).Therefore, that the big and deleterious synthetic cosmetics of some side effect of novel cosmetic raw material substitution are become is a kind of inevitable for modern biological high-technology.The birth of biotechnology cosmetics more and more is subjected to the favor of consumer, and the trend that replaces traditional cosmetics and chemical toilet is arranged greatly, becomes third generation cosmetics.Yet these biotechnology cosmetics that contain bioactive substance (as EGF, IL-2, AHA, hyaluronic acid, skin growth hormone, ferment, Chinese herbal medicine extract and other animals and plants extract etc.) nearly all will adopt the traditional chemical matrix components to make the cream kind goods, usually room temperature preservation.The expert discovers through biology, most bioactive substances (biological technology products) reduced activity or completely lose activity in the cream of cosmetics frost substrate; Under the non-low temperature state (low temperature state refers to 2-8 ℃), the biological activity of many bioactive substances obviously weakens even completely loses, and what really work may be the substrate (preserve moisture, lubricated) of cream frost.Contain protein-based biopolymer of allosome (or xenogenesis) such as collagen protein, elastin laminin, metallothionein (MT), IL-2, hydrolyzed protein enzyme etc. in the part biological technology cosmetics, these polymer substances are difficult to absorb through skin, are difficult to the performance biological action; Or contain the very strong small molecule bioactive material of some zests, as fruit acid (AHA), kojic acid, hyaluronic acid etc., though have well recent cosmetic result, long-term effect is on the knees of the gods.Though contain the cosmetics of intacellin listing is arranged, owing to all adopt the traditional chemical matrix components to make the cream kind goods, so cosmetic result is restricted.
One of purpose of the present invention provides a kind of high-purity, efficient bio-active and uses the micro fetus active composite of high safety; Two of purpose provides a kind of economy, the efficient also preparation method of producing this micro fetus active composite of easy operating; Three of purpose provides the application purpose that micro fetus active composite is used for cosmetics.
Purpose of the present invention realizes by following technical solution: micro fetus active composite of the present invention mainly is composited by Placenta Hominis micro fetus active and embryo liver micro fetus active and line and staff control's micro fetus active, and the content of its main component is (volume %):
Placenta Hominis micro fetus active 3-60
Tire liver micro fetus active 95-5
The micro fetus active 2-35 of line and staff control
The molecular weight of above-mentioned main component is all less than 6000 dalton, and described line and staff control micro fetus active is to form by extracting in the line and staff control of the heart that takes out from the people embryo, thymus, spleen and kidney.
Micro fetus active composite of the present invention is produced by the following method; One, choose qualified people's Placenta Hominis, tire liver and make raw material from heart, thymus, spleen and kidney that the people embryo takes out, promptly select healthy foot monthly output fetal placenta, require fresh totally, 30 ℃ of following room temperatures are placed and are no more than 8 hours.The healthy fetus of select planning fertility induction of labor with water bag, gestational age are 4-8 month.Two, puerpera's liver power checking, HBsAg, HCAb, syphilis antibody and HIV antibody are checked, promptly the puerpera checks that liver function is normal, and all the other antibody inspections all should be negative.Three, Placenta Hominis and tire liver and the heart, thymus, spleen and the kidney that take out from the people embryo cleaned, weigh, shred, are smashed to pieces, homogenate, method feature of the present invention is directly homogenate to be respectively charged into after the homogenate dialyses in the bag filter that dialysis solution is housed and obtains Placenta Hominis, tire liver and line and staff control's micro fetus active respectively, then above-mentioned three kinds of micro fetus actives are mixed in following ratio (volume %): Placenta Hominis micro fetus active 3-60, tire liver micro fetus active 95-5, the micro fetus active 2-35 of line and staff control promptly obtains micro fetus active composite.
Different with conventional method is that the used dialysis solution of the present invention is 0.5% saline.
Be packaged in the special aseptic vial through dialysis gained micro fetus active composite finished product.
Micro fetus active composite of the present invention can be used as cosmetics.
The main component of micro fetus active composite of the present invention is small-molecule peptide and nucleotide, and contains 16 seed amino acids (wherein 8 kinds are essential amino acid), ribose, deoxyribose and DNA, RNA, organic acid, inorganic microelement, micromolecule cell growth factor and multivitamin.Its main character and result of the test are as follows: (1) peptide class content: 1.0-1.5/ml; (2) nucleotide: 260nm has absworption peak (OD
260/ OD
280); (3) molecular weight is less than 6000 dalton; (4) pH value: 6.5-7.0; (5) microbial check (antibacterial culturing): feminine gender; (6) protein test: feminine gender; (7) HBsAg, HCAb, syphilis antibody and HIV antibody are checked: feminine gender; (8) animal toxicity test: mouse peritoneal injection MEAF0.5-1.5ml, observe 1-2 week, mice is still healthy, weight increase, local no dense swollen, incrustation; (9) irritant test: MEAF is splashed into the animal eye conjunctiva, do not have redness, do not have congested phenomenon; (10) hypersensitive test: feminine gender; (11) lamp inspection: clarification, transparent, no turbidity sediment and other foreign body.
The biological activity of micro fetus active composite of the present invention can keep more than 1 year, and is more heat-resisting, do not contain protein, do not have irritated, non-stimulated, have no side effect.
The application of the present invention aspect medical cosmetology is mainly reflected in following three aspects: one, improve local blood circulation, recover skin elasticity.This is one of most important effect in the medical cosmetology.The inventor's application test shows that MEAF can not only improve cell immune function of human body, but also has nutrition local vascular and nerve, improves the effect of local blood circulation, softening skin.Because of not containing macromolecular hormone, enzyme and other protein among the MEAF, be easy to through skin and mucosa absorption, can improve mucocutaneous local blood circulation rapidly, recover skin elasticity, can not cause skin allergy.Two, skin nutrition, elimination fine wrinkle: contain abundant small molecule bioactive material among the MEAF, be easy to by skin absorbs, skin there is very strong Nutrition, many small-molecule substances can improve local blood circulation simultaneously, stimulate epidermis cell differentiation hypertrophy, collagen is synthetic, promotes skin metabolism, therefore has well crease-resistant, face-nursing function.Three, whiten, skin moistening, defying age: contain many beautifying and anti-aging factors such as multiple nature moisturizing factor in the micro fetus active composite of the present invention, the micromolecule cell growth factor, vitamin and micro-micromolecule enzyme and hormone, also has abundant immunoregulatory factor simultaneously, these small molecule bioactive materials have the thing catalytic action than Johnson ﹠ Johnson, can improve the chromolipid metabolism of skin histology significantly, skin care, strengthen the immunologic function of skin, the therapeutical effect that ultraviolet light defense reaction and wound are arranged, the application of local skin, can make skin whitening, tender, ruddy, fine and smooth, increase the elasticity of skin, eliminate the tiny wrinkle of skin, adhere to using the energy slow down aging, remain youthful forever.
Micro fetus active composite of the present invention is owing to be according to a certain percentage Placenta Hominis MEAF, tire liver MEAF and the MEAF of line and staff control to be composited, and the therefore compatible elite of each composition has more efficiently beautifying and anti-aging effect.
Preparation method of the present invention had both reduced the required expensive device of low-temperature centrifugation, had reduced the chance of polluting, and can increase output again.The used dialysis solution of the present invention simultaneously is 0.5% saline, has both avoided the too high shortcoming that influences cosmetic result of sodium chloride content in the normal saline, has overcome pure distilled water again and has crossed the low deficiency that influences dialysis-effect because of osmotic pressure.
The present invention is from the preparation method to the packing of product and store and all to have adopted advanced biological product technology, has guaranteed the tight security of high-purity (pure natural property), efficient bio-active and the use of product.
Provide embodiments of the invention below:
One, micro fetus active composite of the present invention.
Micro fetus active composite of the present invention mainly is composited by Placenta Hominis micro fetus active, tire liver micro fetus active and line and staff control's micro fetus active, and its optimum content is (volume %):
Placenta Hominis micro fetus active 30
Tire liver micro fetus active 50
Line and staff control's micro fetus active 20,
The molecular weight of above-mentioned main component is all less than 6000 dalton.Described line and staff control fetus active factor is to form by extracting in the line and staff control of the heart that takes out from the people embryo, thymus, spleen and kidney.Except that above-mentioned main component, add micro-antiseptic methyl hydroxybenzoate 0.04% (volume) in the complex.Two, the preparation method of micro fetus active composite of the present invention.
(1), producing of Placenta Hominis MEAF:
(1), raw material: select healthy mature newborn baby's Placenta Hominis, require fresh totally, 30 ℃ of following room temperatures are placed and are no more than 8 hours.(2), puerpera's inspection, HBsAg, HCAb, syphilis antibody and HIV antibody are checked: the puerpera checks that liver function should be normal, and the antibody inspection all should be negative.Leave and take placental blood 1ml again, further check HBsAg, HCAb, syphilis antibody and HIV antibody, in case puerpera's omission.(3), use the distilled water flushing Placenta Hominis, cut off fascia, the reuse distilled water cleans 3-4 time, weigh then, shred, add equivalent sterile distilled water, with high-speed tissue mashing machine smash to pieces, homogenate, (4), tissue homogenate is sub-packed in the aseptic conical flask, every bottle is no more than 1000ml and seals the Dou that inclines and place and cross liquid in the refrigerator-freezer and freeze, and melts then.Freeze repeatedly, melt 2-3 time.(5) bag filter is cut into 1.5 meters long (but fill 1kg raw materials), soaks, add the soft lavation of detergent, rinse back reuse sterile distilled water well with tap water and clean 30 minutes sterilizations of conventional high pressure with clear water.(6), with the direct fill of tissue homogenate (about 1kg) in bag filter of melting, use the normal saline flushing outer wall behind the ligation two ends, be contained in the aseptic beaker, add 0.5% saline 400ml/kg, seal and be placed on 4 ℃ of refrigerators and dialysed 72 hours.(7), collect dialysis solution, survey pH value and be adjusted to 6.5-7.0 with reagent paper, the malleation heat sterilization adds concentration and is 0.04% Methyl p-hydroxybenzoate, with the aseptic conical flask packing of 500ml, seals and is placed on 4 ℃ of refrigerators and preserves.
(2), producing of tire liver and the MEAF of line and staff control:
(1), raw material: the healthy fetus of select planning fertility induction of labor with water bag, gestational age are 4-8 month, and the placement of low temperature below 30 ℃ is no more than 8 hours.(2), the puerpera checks that liver function should be normal, HBsAg, HCAb, syphilis antibody and the inspection of HIV antibody all should be negative.(3), use the distilled water flushing embryo, clean the bloodstain of body surface, after the conventional body surface sterilization, take out liver, heart, thymus, spleen and kidney through thoracoabdominal incision, leave and take fetal blood 1ml and further check HBsAg, HCAb, syphilis antibody and HIV antibody, in case puerpera's omission.Above-mentioned embryonal tissue be cut into the flask (wherein liver is placed in the bottle, and heart, thymus, spleen and kidney are placed in another bottle) of putting 250ml behind the fragment into, put into cryogenic refrigerator then and preserve (below 30 ℃) (4), respectively.(5), thaw after, weigh respectively, add equivalent 0.5% saline, with high-speed tissue mashing machine smash to pieces, homogenate, each one minute (using the frozen water cryostat at interval in 5 minutes), totally four times.Following steps are produced (4)-(7) of step with Placenta Hominis MEAF.Tire hepatic tissue extract is tire liver MEAF, and heart, thymus, spleen and renal tissue mixed extract are the MEAF of line and staff control.
Three, the purposes of micro fetus active composite of the present invention.
The present invention can be used as cosmetics, has fairly obvious beautifying and anti-aging effect.
The clinical principium applicable cases: select volunteer 51 examples to test, male's 15 examples, women's 36 examples, the age is 23-46 year, average 29 years old.Usage: behind cleansing milk cleaning facial skin, directly use MEAF complex (injection type) to embrocate skin of face, sooner or later respectively once, and each 0.5-1.0ml, 30 days is 1 course of treatment.With self is not matched group with MEAF complex previous crops.The result is: after 1-2 the course of treatment on probation, skin coarse, that deceive polishes, delicate, fine wrinkle disappears 48 examples (94.1%), and erythrosis moistens soft 47 examples (92.2%), and face has freckle or chloasma 5 examples, and color is all obviously thin out.Any toxic and side effects (comprising contact dermatitis or allergic dermatitis etc.) appears in none example.The result shows, this bioactive substance has tangible Nutrition because of containing abundant polypeptide, nucleotide, aminoacid, ribonucleic acid, organic acid and inorganic microelement etc. to application on human skin, can promote the metabolism of skin histology simultaneously, significantly improve and strengthen the microcirculation of skin of face and the function of sebaceous gland, have sure beauty treatment and anti-aging effects.
Claims (4)
1, a kind of micro fetus active composite is characterized in that it mainly is composited by tire liver micro fetus active, Placenta Hominis micro fetus active and line and staff control's micro fetus active, and the content of its Main Ingredients and Appearance is (volume %):
Placenta Hominis micro fetus active 3-60
Tire liver micro fetus active 95-5
The micro fetus active 2-35 of line and staff control,
The molecular weight of above-mentioned Main Ingredients and Appearance is less than 6000 dalton;
Above-mentioned tire liver micro fetus active, Placenta Hominis micro fetus active and line and staff control's micro fetus active are produced by following method:
A, the qualified people's Placenta Hominis of selection and people's tire liver and the heart, thymus, spleen and the kidney that take out from the people embryo are made raw material;
B, respectively raw material described in a weighed, shred, smash to pieces, homogenate;
C, homogenate is respectively charged into dialyses in the bag filter that dialysis solution is housed and obtain Placenta Hominis, tire liver and line and staff control's micro fetus active respectively;
Dialysis solution described in the step c is 0.5% saline.
2, preparation method according to the micro fetus active composite of claim 1, it is characterized in that it comprises the steps: (one), the heart of selecting qualified people's Placenta Hominis and people's tire liver and from the people embryo, taking out, thymus, spleen and kidney are made raw material, (2), respectively Placenta Hominis and tire liver are reached the heart that takes out from the people embryo, thymus, spleen and kidney clean, weigh, shred, smash to pieces, homogenate, (3), directly homogenate is respectively charged into after the homogenate and dialyses in the bag filter that dialysis solution is housed and obtain Placenta Hominis respectively, the tire liver, with line and staff control's micro fetus active, (4), above-mentioned three kinds of micro fetus actives are mixed in following ratio (volume %): Placenta Hominis micro fetus active 3-60, tire liver micro fetus active 95-5, the micro fetus active 2-35 of line and staff control;
Dialysis solution described in the step (three) is 0.5% saline.
3, according to the application of the micro fetus active composite of claim 1 as cosmetics.
4, the preparation method of micro fetus active composite according to claim 2 is characterized in that being packaged in the aseptic vial through dialysis gained finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96111452A CN1074280C (en) | 1996-11-26 | 1996-11-26 | Micro fetus active composite, its preparing process and application in cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96111452A CN1074280C (en) | 1996-11-26 | 1996-11-26 | Micro fetus active composite, its preparing process and application in cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1181242A CN1181242A (en) | 1998-05-13 |
| CN1074280C true CN1074280C (en) | 2001-11-07 |
Family
ID=5121121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96111452A Expired - Fee Related CN1074280C (en) | 1996-11-26 | 1996-11-26 | Micro fetus active composite, its preparing process and application in cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1074280C (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102097A (en) * | 1993-10-29 | 1995-05-03 | 泰安市中医医院 | Human body early embryo factor and preparation method and its application in pharmaceutics |
| CN1114199A (en) * | 1994-06-30 | 1996-01-03 | 李滨胜 | Process for extracting embryo factor of fowls and medicine usage |
-
1996
- 1996-11-26 CN CN96111452A patent/CN1074280C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102097A (en) * | 1993-10-29 | 1995-05-03 | 泰安市中医医院 | Human body early embryo factor and preparation method and its application in pharmaceutics |
| CN1114199A (en) * | 1994-06-30 | 1996-01-03 | 李滨胜 | Process for extracting embryo factor of fowls and medicine usage |
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| Publication number | Publication date |
|---|---|
| CN1181242A (en) | 1998-05-13 |
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