CN107412600B - Extraction method of avenanthramides, spray and preparation method thereof - Google Patents
Extraction method of avenanthramides, spray and preparation method thereof Download PDFInfo
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Abstract
An extraction method of avenanthramides, a spray and a preparation method thereof, which relate to the field of dermatitis treatment. A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 50-70 deg.C for 1-3h, purifying the coarse extract with macroporous resin, and concentrating. The method can effectively extract avenanthramide. A spray is prepared from raw materials including the first main material including avenanthramide, 1, 2-pentanediol, 1, 2-hexanediol and dimethyl glycyrrhizinate, the second main material and adjuvants. The second main material comprises allantoin and water. The adjuvants include sodium hyaluronate, 1, 2-hexanediol and water. The spray adopts avenanthramides as main active ingredients, has the effect of repairing irritant dermatitis, and is natural and free of toxic and side effects.
Description
Technical Field
The invention relates to the field of dermatitis treatment, and particularly relates to an extraction method of avenanthramides, a spray and a preparation method thereof.
Background
Glucocorticoid called opium, abuse of cosmetic cosmetics or ointments which imply glucocorticoid for a long time will cause toxic side effects of the hormone, resulting in severe skin diseases, so-called hormone-dependent dermatitis or hormone-dependent face. The skin lesions are characterized by thinning, atrophy, flushing, telangiectasia, acne, rosacea-like changes and the like of the facial skin, and subjective symptoms are mainly burning, itching, pain, dryness, desquamation and tension. The disease generally has a long course of disease, the disease is difficult to cure repeatedly, the life quality of the patient is greatly reduced, the clinical treatment is relatively troublesome, and the current treatment means mainly causes the skin to adapt by continuously reducing the dosage of hormone. In addition, the external application of the Chinese herbal medicine ointment, the medicinal oil and the spray can be used for treating the psoriasis. The ointment and the medicinal oil are mostly used at home, and the medicine is taken by hands or cotton swabs, so that the medicine taking process is inconvenient, and the patient is susceptible to pain in the medicine taking process. The traditional treatment spray needs to be added with preservative because of contacting with air, and inevitably introduces an irritant.
Disclosure of Invention
The first purpose of the invention is to provide an extraction method of avenanthramides, which can effectively extract the avenanthramides.
The second purpose of the invention is to provide a spray which adopts avenanthramides as a main active ingredient, has the efficacy of repairing irritant dermatitis, is natural and has no toxic or side effect.
The third purpose of the invention is to provide a preparation method of the spraying agent, which is simple and easy to operate and can better preserve the active ingredients of each component.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
The invention provides an extraction method of avenanthramide, which comprises the following steps:
extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 50-70 deg.C for 1-3h, purifying the coarse extract with macroporous resin, and concentrating.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 10-30 parts by weight of avenanthramides prepared by the extraction method of avenanthramides, 1-7 parts by weight of 1, 2-pentanediol, 1-7 parts by weight of 1, 2-hexanediol and 1-5 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 2-5 parts of allantoin and 25-40 parts of water by weight. The auxiliary materials comprise 0.1 to 0.7 weight portion of sodium hyaluronate, 1 to 7 weight portions of 1, 2-hexanediol and 15 to 20 weight portions of water.
A method for preparing a spray, comprising: mixing the second main material, heating to 75-90 deg.C, keeping the temperature for 10-30min, cooling to 55-70 deg.C, adding the mixed adjuvants, homogenizing, cooling to 40-50 deg.C, and mixing with the first main material.
The embodiment of the invention has the beneficial effects that: a method for extracting avenanthramides from oat bran comprises extracting oat bran with a mixture of 1, 2-pentanediol and 1, 2-hexanediol, purifying with macroporous resin, and concentrating. The method can effectively extract oat alkaloid from oat bran.
A spray comprises oat alkaloid as main active ingredient, and adjuvants including urocystine and glycyrrhizic acid dimethyl ester, and has good therapeutic effect on repairing hormone-dependent dermatitis. The oat alkaloid is extracted from oat bran, and is natural and has no toxic or side effect.
A preparation method of spray is simple and easy to operate, different raw materials are mixed at different temperatures, and effective components of the components can be well preserved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a flow chart of the present invention;
fig. 2 is a schematic diagram of a dual package structure provided by the present invention.
Icon: 100-binary packaging; 110-a tank body; 111-can mouth; 120-air bag; 130-an aerosol assembly; 131-a cover body; 132-a spray tube; 133-a valve assembly; 140-shower head.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are conventional products available commercially.
The method for extracting avenanthramides, the spray and the method for preparing the spray according to the embodiments of the present invention are described in detail below.
A method for extracting avenanthramides comprises:
extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 50-70 deg.C for 1-3h, purifying the coarse extract with macroporous resin, and concentrating.
The method can effectively extract oat alkaloid from oat bran. Preferably, the weight ratio of 1, 2-pentanediol to 1, 2-hexanediol is 1:0.3 to 3. In alternative embodiments, the weight ratio of 1, 2-pentanediol to 1, 2-hexanediol is from 1:1 to 2. In addition, in the embodiment of the invention, in order to better purify the extract, the macroporous resin is macroporous resin AB-8.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 10-30 parts by weight of avenanthramides prepared by the extraction method of avenanthramides, 1-7 parts by weight of 1, 2-pentanediol, 1-7 parts by weight of 1, 2-hexanediol and 1-5 parts by weight of dimethyl glycyrrhizinate. The second main material comprises 2-5 parts of allantoin and 25-40 parts of water by weight. The auxiliary materials comprise 0.1 to 0.7 weight portion of sodium hyaluronate, 1 to 7 weight portions of 1, 2-hexanediol and 15 to 20 weight portions of water.
The avenanthramide is used as the main active ingredient of the spray, has the efficacy of repairing the hormone-dependent dermatitis, and has better treatment effect. The oat alkaloid is extracted from oat bran, and is natural and has no toxic or side effect.
Allantoin, also known as 5-ureylethanolamide, ureidoacetolactam, ureidohydantoin, uretidione, is a hydantoin derivative. Allantoin has physiological functions of promoting cell growth, accelerating wound healing, softening keratin and the like, and has certain treatment effect on hormone-dependent dermatitis.
The glycyrrhizic acid dimethyl ester has the effects of bacteriostasis, inflammation diminishing, detoxification, allergy resistance and the like.
The oat alkaloid is used as the main active ingredient of the spray, and the allantoin and the glycyrrhizic acid dimethyl are used as auxiliary materials, so that the spray has a good treatment effect on repairing the hormone-dependent dermatitis.
In an embodiment of the invention, the water in the spray feedstock is high purity water. The impurities in the high-purity water are few, and the prepared spray has better effect.
A method for preparing a spray, comprising: mixing the second main material, heating to 75-90 deg.C, keeping the temperature for 10-30min, cooling to 55-70 deg.C, adding mixed adjuvants, homogenizing, cooling to 40-50 deg.C, and mixing with the first main material. Please refer to fig. 1.
Specifically, the high-purity water and the allantoin in the second main material are sequentially added into a clean emulsifying pot, stirred, heated to 75-90 ℃, and kept for 10-30min, so that the urea sac can be completely dissolved. The first temperature of 75-90 ℃ can ensure that the urea capsule can be well dissolved and the structure of the urea capsule cannot be damaged due to too high temperature. Preferably, the first temperature is 80-85 ℃.
Keeping the temperature for 10-30min, cooling to 55-70 deg.C, adding pre-dispersed sodium hyaluronate, 1, 2-hexanediol and ultrapure water, and homogenizing to completely dissolve and disperse. Since sodium hyaluronate has thickening effect, it is preferably added after being pre-dispersed with 1, 2-hexanediol. And the sodium hyaluronate can be well dissolved within the temperature range of 55-70 ℃ without damaging the structure. Preferably, the second temperature is 60-65 ℃.
Cooling to a third temperature of 40-50 deg.C, adding the first main material, and mixing. The avenanthramides and glycyrrhizic acid diformyl in the first main material can be dissolved well in the temperature range, and the effective components of the first main material cannot be damaged.
Embodiments of the present invention also include packaging the aerosol using the dual package 100. The dual package 100 separately packages the spray and propellant in separate containers without contaminating the spray. Specifically, referring to fig. 2, the dual package 100 includes a can 110, a bladder 120, an aerosol assembly 130, and a spray head 140.
The tank 110 has a tank opening 111, and compressed air is provided in the tank 110. The tank 110 may be an aluminum tank or an iron tank, and is not limited to other ways not listed in the embodiment.
The spray head 140 is covered on the spray pipe 132, and the sunscreen spray in the airbag 120 can be sprayed by pressing the spray head 140.
In addition, the dual package 100 may further include a cover cap (not shown) covering the nozzle 140 to prevent the nozzle 140 from being contaminated by the outside.
Traditional sprays inevitably introduce an irritation source due to the need for preservatives to be added in contact with the air, and unitary packaged sprays need to be mixed directly with the propellant, with the risk of introducing contamination and irritation sources. By virtue of the binary package 100, the avenanthramides, as active ingredients, are separated from the propellant without contaminating the active material. And the binary package 100 uses air as a propellant without the risk of explosion. The spray of the invention is natural and safe without adding preservatives.
The spray packaged by the binary package 100 can be carried about, does not need to touch the skin with hands when in use, and can quickly relieve the discomfort of the skin.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 55 deg.C for 2h, purifying the coarse extract with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1:1.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 10 parts by weight of avenanthramide, 2 parts by weight of 1, 2-pentanediol, 4 parts by weight of 1, 2-hexanediol and 3 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 3 parts of allantoin and 30 parts of ultrapure water. The auxiliary materials comprise 0.2 weight part of sodium hyaluronate, 2 weight parts of 1, 2-hexanediol and 15 weight parts of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 80 ℃, keeping the temperature for 20min, then cooling to 60 ℃ at the second temperature, adding the pre-dispersed uniform auxiliary materials, homogenizing, cooling to 42 ℃, adding the first main material, and mixing.
Example 2
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 60 deg.C for 2.5h for 1 g/5 mL to obtain a crude extract, purifying with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 0.3.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 15 parts by weight of avenanthramide, 3 parts by weight of 1, 2-pentanediol, 2 parts by weight of 1, 2-hexanediol and 4 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 2 parts of allantoin and 25 parts of ultrapure water by weight. The auxiliary materials comprise 0.2 weight part of sodium hyaluronate, 3 weight parts of 1, 2-hexanediol and 18 weight parts of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 75 ℃, keeping the temperature for 30min, then cooling to 55 ℃ at the second temperature, adding the pre-dispersed uniform auxiliary materials, homogenizing, cooling to 45 ℃ at the third temperature, and adding the first main material for mixing.
Example 3
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 50 deg.C for 3h, purifying the coarse extract with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1:2.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 20 parts by weight of avenanthramide, 4 parts by weight of 1, 2-pentanediol, 1 part by weight of 1, 2-hexanediol and 2 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 4 parts of allantoin and 28 parts of ultrapure water. The auxiliary materials comprise 0.1 part by weight of sodium hyaluronate, 4 parts by weight of 1, 2-hexanediol and 16 parts by weight of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 85 ℃, keeping the temperature for 10min, then cooling to 65 ℃ of the second temperature, adding the pre-dispersed uniform auxiliary materials, homogenizing, cooling to 40 ℃ of the third temperature, adding the first main material, and mixing.
Example 4
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 65 deg.C for 1.5h for 1 g/10 mL to obtain a crude extract, purifying with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 2.5.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 18 parts by weight of the extracted avenanthramides, 5 parts by weight of 1, 2-pentanediol, 5 parts by weight of 1, 2-hexanediol and 5 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 2.5 parts of allantoin and 32 parts of ultrapure water. The auxiliary materials comprise 0.4 part by weight of sodium hyaluronate, 5 parts by weight of 1, 2-hexanediol and 20 parts by weight of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 78 ℃, keeping the temperature for 25min, then cooling to 58 ℃ of the second temperature, adding the pre-dispersed uniform auxiliary materials for homogenizing, and adding the first main material for mixing when the temperature is reduced to 43 ℃ of the third temperature.
Example 5
A method for extracting avenanthramides comprises: extracting oat bran with a mixture of 1, 2-pentanediol and 1, 2-hexanediol at 62 deg.C for 100min for 1 g/6 mL to obtain a crude extract, purifying with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 1.5.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 12 parts by weight of avenanthramide, 1 part by weight of 1, 2-pentanediol, 4 parts by weight of 1, 2-hexanediol and 3 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 3.5 parts of allantoin and 40 parts of ultrapure water. The auxiliary materials comprise 0.5 part by weight of sodium hyaluronate, 1 part by weight of 1, 2-hexanediol and 17 parts by weight of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 88 ℃, keeping the temperature for 15min, then cooling to 68 ℃ of the second temperature, adding the pre-dispersed uniform auxiliary materials for homogenizing, cooling to 48 ℃ of the third temperature, and adding the first main material for mixing.
Example 6
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 70 deg.C for 1h, purifying the coarse extract with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 0.5.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 22 parts by weight of avenanthramide, 3 parts by weight of 1, 2-pentanediol, 6 parts by weight of 1, 2-hexanediol and 5 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 5 parts of allantoin and 25 parts of ultrapure water. The auxiliary materials comprise 0.3 weight part of sodium hyaluronate, 3 weight parts of 1, 2-hexanediol and 16 weight parts of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 82 ℃, keeping the temperature for 18min, then cooling to 62 ℃ of the second temperature, adding the pre-dispersed uniform auxiliary materials for homogenizing, cooling to 45 ℃ of the third temperature, and adding the first main material for mixing.
Example 7
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 58 deg.C for 140min, purifying the coarse extract with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 3.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 30 parts by weight of the extracted avenanthramides, 6 parts by weight of 1, 2-pentanediol, 7 parts by weight of 1, 2-hexanediol and 2 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 2 parts of allantoin and 23 parts of ultrapure water. The auxiliary materials comprise 0.6 weight part of sodium hyaluronate, 6 weight parts of 1, 2-hexanediol and 19 weight parts of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 90 ℃, keeping the temperature for 10min, then cooling to 70 ℃ at the second temperature, adding the pre-dispersed uniform auxiliary materials, homogenizing, cooling to 50 ℃ at the third temperature, and adding the first main material for mixing.
Example 8
A method for extracting avenanthramides comprises: extracting oat bran with a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol at 60 deg.C for 80min for 1g:7mL to obtain a crude extract, purifying with macroporous resin AB-8, and concentrating. Wherein the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1:2.
A spray is mainly prepared from raw materials including a first main material, a second main material and an auxiliary material, wherein the first main material comprises 25 parts by weight of avenanthramide, 7 parts by weight of 1, 2-pentanediol, 3 parts by weight of 1, 2-hexanediol and 4 parts by weight of glycyrrhizic acid dimethyl. The second main material comprises 3 parts of allantoin and 28 parts of ultrapure water. The auxiliary materials comprise 0.7 weight part of sodium hyaluronate, 7 weight parts of 1, 2-hexanediol and 15 weight parts of ultrapure water.
The preparation method of the spray comprises the following steps: weighing the raw materials according to the proportion, sequentially adding the high-purity water and the allantoin in the second main material into a clean emulsifying pot, stirring, heating to 85 ℃, keeping the temperature for 15min, then cooling to 58 ℃ as the second temperature, adding the pre-dispersed uniform auxiliary materials for homogenizing, cooling to 42 ℃, adding the first main material, and mixing.
Comparative example 1
The preparation method and raw materials of the spray of comparative example 1 are the same as those of example 2, and the difference is only that the raw material proportion of the spray is different. The raw materials of the spray of this comparative example included: the first main material, the second main material and the auxiliary materials, wherein the first main material comprises: 8 parts of oat alkaloid, 0.5 part of 1, 2-pentanediol, 0.8 part of 1, 2-hexanediol and 5.5 parts of glycyrrhizic acid dimethyl. The second main material comprises 1.5 parts of allantoin and 15 parts of ultrapure water. The auxiliary materials comprise 0.05 weight part of sodium hyaluronate, 7.3 weight parts of 1, 2-hexanediol and 22 weight parts of ultrapure water.
Comparative example 2
The preparation method and raw materials of the spray of comparative example 2 are the same as those of example 2, and the difference is only that the raw material proportion of the spray is different. The raw materials of the spray of this comparative example included: the first main material, the second main material and the auxiliary materials, wherein the first main material comprises: 32 parts of oat alkaloid, 7.3 parts of 1, 2-pentanediol, 7.2 parts of 1, 2-hexanediol and 1.5 parts of glycyrrhizic acid dimethyl. The second main material comprises 6 parts of allantoin and 45 parts of ultrapure water. The auxiliary materials comprise 0.8 weight part of sodium hyaluronate, 0.8 weight part of 1, 2-hexanediol and 12 weight parts of ultrapure water.
Test example 1
The therapeutic drug is: the sprays of examples 1 to 8 and comparative examples 1 and 2 were packed in a binary pack to obtain experimental drugs 1 to 8 and comparative drugs 1 to 2, respectively.
Animals: 110 ICR mice, female, with a weight of 22-28g, purchased from Experimental animals center in Zhejiang province, production license number: SCXK (Zhe) 2017-.
The test process comprises the following steps: ICR mice 110 were randomly divided into 11 groups of 10 mice each. Mice were fasted for 12 hours, and then the abdomen of each mouse was shaved and then applied with fluorescein-5-isothiocyanate (solvent: acetone and dibutyl phthalate in a weight ratio of 1: 1) at a mass concentration of 0.5% to cause an allergic reaction. One week later, the mice were coated with fluorescein-5-isothiocyanate at a mass concentration of 0.5% in the left ear and left without treatment in the right ear. After 1 day, the average thickness of the left and right ears was measured. Then, the experimental drugs 1-8, the comparative drugs 1-2 and the untreated mice were sprayed on the left ear of the mice of groups 1-8, 9-10 and 11, respectively. Three times a day, continuously spraying for 3 days. Then, the mice are killed by dislocation of cervical vertebrae, the left and right ears of the mice with the diameter of 8mm are punched, the average thickness of the left ear and the right ear is measured again, and the ear swelling degree before and after administration is calculated, wherein the ear swelling degree is the left ear thickness/the right ear thickness. The results are reported in table 1.
TABLE 11-11 ear swelling before and after administration of mice
As can be seen from the results in Table 1, the swelling degree of the ears of the mice in groups 1 to 8 was greatly reduced after administration, indicating that the sprays of examples 1 to 8 of the present invention had significant anti-inflammatory effects. In addition, the sprays of groups 9-10 have certain anti-inflammatory effects although the effects are not as good as those of groups 1-8, which indicates that the raw material ratio of the spray of the embodiment of the invention has an outstanding effect on treating dermatitis.
Test example 2
Test subjects: selecting a patient with a history of hormone for more than one month, and the original disease or skin lesion is relapsed or aggravated after the hormone is stopped for 2-10 days; subjective symptoms are manifested as itching, burning sensation, dry and wrinkled sensation, pain; patients with objective symptoms of inflammatory skin rash or abscess, erythema, flushing, edema, coarse hair pores, pigmentation, epidermal atrophy, etc. were selected as test subjects, and the test subjects were divided into 1-10 groups of 50 persons each, each group being 20 to 45 years old.
The therapeutic drug is: the sprays of examples 1 to 8 and comparative examples 1 and 2 were packed in a binary pack to obtain experimental drugs 1 to 8 and comparative drugs 1 to 2, respectively.
The experimental process comprises the following steps: the treatment effect was recorded after 10 days by spraying the patients of groups 1-8 with the experimental drug 1-8 and groups 9-10 with the comparative drug 1-2, the results of which are recorded in table 2.
TABLE 21-10 therapeutic Effect of the patients after administration
As can be seen from the results in Table 2, the treatment effect was better in the patients of groups 1 to 8 after administration and slightly worse in the groups 9 to 10, indicating that the spray of the present invention has a better treatment effect on the hormone-dependent dermatitis.
In summary, in the method for extracting avenanthramides in the embodiment of the present invention, mixed solution of 1, 2-pentanediol and 1, 2-hexanediol is used to extract oat bran, and then the oat bran is purified and concentrated by macroporous resin. The method can effectively extract oat alkaloid from oat bran.
According to the spray provided by the embodiment of the invention, the oat alkaloid is used as a main active ingredient of the spray, and the allantoin and the glycyrrhizic acid dimethyl are used as auxiliary materials, so that the spray has a good treatment effect on repairing the hormone-dependent dermatitis. The oat alkaloid is extracted from oat bran, and is natural and has no toxic or side effect.
The preparation method of the spray provided by the embodiment of the invention is simple and easy to operate, different raw materials are mixed at different temperatures, and the effective components of the components can be well preserved.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without any inventive step, are within the scope of the present invention.
Claims (6)
1. The spray is characterized in that the raw materials are prepared from a first main material, a second main material and an auxiliary material, wherein the first main material comprises 10-30 parts by weight of avenanthramides prepared by an avenanthramides extraction method, 1-7 parts by weight of 1, 2-pentanediol, 1-7 parts by weight of 1, 2-hexanediol and 1-5 parts by weight of dimethyl glycyrrhizinate; the second main material comprises 2-5 parts by weight of allantoin and 25-40 parts by weight of ultrapure water; the auxiliary materials comprise 0.1 to 0.7 weight part of sodium hyaluronate, 1 to 7 weight parts of 1, 2-hexanediol and 15 to 20 weight parts of ultrapure water;
the extraction method of the avenanthramide comprises the following steps: extracting oat bran by using a mixed solution of 1, 2-pentanediol and 1, 2-hexanediol to obtain a crude extract, wherein the extraction temperature is 50-70 ℃, the extraction time is 1-3 hours, the ratio of the oat bran to the mixed solution is 1g:5-10mL, and purifying and concentrating the crude extract by using macroporous resin AB-8; the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 0.3-3.
2. The spray according to claim 1, wherein in the method for extracting avenanthramides, the weight ratio of the 1, 2-pentanediol to the 1, 2-hexanediol is 1: 1-2.
3. A method of preparing a spray according to claim 1 or 2, comprising: mixing the second main material, heating to 75-90 deg.C, keeping the temperature for 10-30min, cooling to 55-70 deg.C, adding the mixed adjuvants, homogenizing, cooling to 40-50 deg.C, and mixing with the first main material.
4. The method for preparing a spray according to claim 3, wherein the first temperature is 80-85 ℃.
5. The method of preparing a spray according to claim 3, wherein the second temperature is 60-65 ℃.
6. The method of claim 3, further comprising packaging the spray using a dual package.
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| PCT/CN2017/109786 WO2019033585A1 (en) | 2017-08-15 | 2017-11-07 | Method for extracting oat alkaloid, and spray and preparation method therefor |
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| CN109970593B (en) * | 2019-03-14 | 2019-11-08 | 北京工商大学 | The extracting method and its extract of a kind of oat extract and application |
| CN110478444A (en) * | 2019-08-29 | 2019-11-22 | 深圳市兰亭科技股份有限公司 | A kind of oat total alkaloid and a kind of composition and preparation method thereof of anti-skin allergy |
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