CN107410668A - A kind of method that yeast polypeptides chelated copper is prepared using beer waste yeast - Google Patents
A kind of method that yeast polypeptides chelated copper is prepared using beer waste yeast Download PDFInfo
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- CN107410668A CN107410668A CN201710435173.XA CN201710435173A CN107410668A CN 107410668 A CN107410668 A CN 107410668A CN 201710435173 A CN201710435173 A CN 201710435173A CN 107410668 A CN107410668 A CN 107410668A
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- yeast
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/348—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins obtained from waste materials
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Husbandry (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Inorganic Chemistry (AREA)
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Abstract
The invention provides a kind of method that yeast polypeptides chelated copper is prepared using beer waste yeast, this method dries the steps such as finished product by the chelating of pretreatment, the extraction of yeast polypeptides, yeast polypeptides and mantoquita, removal of impurities, obtains yeast polypeptides chelated copper powder product.The yeast polypeptides chelating copper products chelated copper content obtained in this method is high, and copper chelation percent is high, and albumen peptide activity is high, and this method makes beer waste yeast turn waste into wealth, and operating procedure is simple, and cost is relatively low, and new approach is opened up to improve its added value.
Description
Technical field
The present invention relates to a kind of method that yeast polypeptides chelated copper is prepared using beer waste yeast, belong to feed addictive skill
Art field.
Background technology
Brewing industry development in China is swift and violent, with the continuous growth of beer production, the various discarded objects in production process
It is consequently increased rapidly, maximum of which discarded object is exactly beer waste yeast, and its discharge capacity accounts for the 2%-3% of beer production.Beer
Waste yeast is nutritious, be by other material compositions such as 40-60% protein, 35%-40% carbohydrate and nucleic acid,
It is a kind of single cell protein resource well.At present, traditional processing method is handled with discharge of wastewater or as feed, so
A large amount of wastes of resource are not only caused, also bring certain pollution to ecological environment.Improve beer waste yeast recovery utilization rate
And it is the main thought to solve the above problems to open up its higher value application approach.
Polypeptide chelate copper is a kind of new benefit copper products of in the market, and not only whole physiology with trace copper are made for it
With, it may have a series of activity of polypeptide, such as promote immunologic function, improve hormonal activity, antiviral etc..Due to polypeptide chelate
Copper is more stable compared with copper amino acid chelate, has absorption rate high；The features such as palatability is strong, and toxic side effect is small, it has also become feed
The research of additive in the market and the focus of concern.
Chelatropic reaction generation yeast polypeptides chelated copper is carried out from the polypeptide of beer waste yeast extraction with mantoquita to not only solve
Beer waste yeast recovery utilization rate and higher value application problem, while it is significant also to develop green animal husbandry to China.Through inspection
The rope country has no the relevant report that yeast polypeptides chelated copper is prepared using beer waste yeast at present.
How a kind of preparation method that using beer waste yeast prepares yeast polypeptides chelated copper is provided, and this method is simply easy
Row, cost are low, can make full use of beer waste yeast resource, be that the present invention needs to solve the problems, such as.
The content of the invention
Based on above mentioned problem, the invention provides a kind of method that yeast polypeptides chelated copper is prepared using beer waste yeast,
The preparation technology is to chelate to obtain yeast polypeptides chelated copper with mantoquita by extracting polypeptide from beer waste yeast.
The concrete technical scheme that the present invention uses is as follows：
A kind of method that yeast polypeptides chelated copper is prepared using beer waste yeast, it specifically comprises the following steps：
（1）Pretreatment：Beer waste yeast liquid carries out broken wall treatment through ultrasonic system, retains supernatant after centrifugation, adds appropriate carbon
Sour hydrogen sodium carries out de- suffering reason, and pretreatment fluid is made；
（2）The extraction of yeast polypeptides：After pretreatment fluid adjusts pH and temperature conditionss, add appropriate protease and carry out enzyme digestion reaction,
Yeast polypeptides solution is generated after enzyme deactivation；
（3）The chelating of yeast polypeptides and mantoquita：The pH and temperature of yeast polypeptides solution are adjusted, the mantoquita for adding appropriate ratio is carried out
Chelatropic reaction；
（4）Finished product is dried in removal of impurities：After chelatropic reaction terminates, chelating liquid is removed free copper ion and carried out dense by nanofiltration system
Contracting, through high temperature spray-drying, obtains yeast polypeptides chelated copper powder product.
Yeast polypeptides chelated copper preparation method described above, the step（1）Middle supersound process condition is 30-50kHZ,
Ultrasonic 1-2h, centrifugal condition 4000-6000r/min, centrifuge 15-30min, and sodium acid carbonate addition is waste yeast liquid quality
0.5%-1.0%, normal-temperature reaction 0.5-2h.
Yeast polypeptides chelated copper preparation method described above, the step（2）The protease of middle addition is Papain
Enzyme, enzymatic hydrolysis condition：Enzyme addition be pretreatment fluid quality 0.08%-0.15%, pH6-8,30-40 DEG C of temperature, time 12-24h,
Enzyme deactivation condition is 90 DEG C of temperature, time 30min.
Yeast polypeptides chelated copper preparation method described above, the step（3）The addition of middle mantoquita is polypeptide solution
The 10%-15% of quality, chelating condition are：PH6.5-7.5,55-65 DEG C of temperature, wherein time 2-4h, mantoquita are copper sulphate, chlorination
The one or two of copper.
Yeast polypeptides chelated copper preparation method described above, the step（4）It is middle chelating liquid by after nanofiltration system its
Solid content is controlled in the range of 40%-50%, and spray drying condition is：190 DEG C -210 DEG C of inlet temperature, outlet temperature 90
DEG C -100 DEG C, product index is chelating copper content 8-12%, copper chelation percent 88-94%, content of peptides 65-70%.
The technology of the present invention has following technical advantage：Using protease method degraded Yeast protein, gained albumen in the present invention
Peptides extraction rate is high, and copper chelation percent is high during chelating, and whole preparation process operating procedure is simple, exploitativeness is strong, cost compared with
It is low, really realize the higher value application to beer waste yeast.
Embodiment
The present invention is further described below by way of specific embodiment, but described embodiment does not limit this in any way
The scope of patent protection of invention.
Embodiment 1：Beer waste yeast liquid 1000kg is pumped into ultrasonic treatment systems, 40kHZ ultrasonications processing 1.0h,
After supersound process terminates, 30min is centrifuged through 4000r/min supercentrifuges, supernatant is pumped into retort, adds waste yeast liquid
The sodium acid carbonate of quality 0.5%, stirring system is opened, reacts 1.0h under normal temperature condition.After reaction terminates, pretreatment fluid pH is adjusted
Value 7.0, the papain of pretreatment fluid quality 0.1% is added, open heating and stirring system, be warming up to 30 DEG C and protect
Temperature, enzyme digestion reaction 24h.After reaction terminates, 90 DEG C are warming up to, enzyme deactivation 30min, obtains yeast polypeptides solution.Yeast polypeptides are molten
Liquid is cooled to 60 DEG C and is incubated, and it is 7.0 to keep pH, adds the copper sulphate of polypeptide solution quality 10%（Five water）, chelatropic reaction
3.0h.Liquid is chelated by nanofiltration system, a small amount of free copper ion is removed and is concentrated into solid content 45%, carried out through drying tower high
Temperature spray drying, 200 DEG C of inlet temperature, 90 DEG C of outlet temperature obtain yeast polypeptides chelated copper, and its product index is：Chelated copper
Content 12.8%, copper chelation percent 93.7%, content of peptides 66%.Meanwhile the waste liquid containing free copper ion adds caused by nanofiltration system
Sodium hydrate solid, copper ion is precipitated, be separated by filtration Kocide SD and precipitate and focus on.
Embodiment 2：Beer waste yeast liquid 5000kg is pumped into ultrasonic treatment systems, 40kHZ ultrasonications processing 1.0h,
After supersound process terminates, 15min is centrifuged through 6000r/min supercentrifuges, supernatant is pumped into retort, adds waste yeast liquid
The sodium acid carbonate of quality 1.0%, stirring system is opened, reacts 0.5h under normal temperature condition.After reaction terminates, pretreatment fluid pH is adjusted
Value 7.0, the papain of pretreatment fluid quality 0.1% is added, open heating and stirring system, be warming up to 30 DEG C and protect
Temperature, enzyme digestion reaction 24h.After reaction terminates, 90 DEG C are warming up to, enzyme deactivation 30min, obtains yeast polypeptides solution.Yeast polypeptides are molten
Liquid is cooled to 60 DEG C and is incubated, and it is 7.0 to keep pH, adds the copper sulphate of polypeptide solution quality 15%（Five water）, chelatropic reaction
4.0h.Liquid is chelated by nanofiltration system, a small amount of free copper ion is removed and is concentrated into solid content 49%, carried out through drying tower high
Temperature spray drying, 200 DEG C of inlet temperature, 90 DEG C of outlet temperature obtain yeast polypeptides chelated copper, and its product index is：Chelated copper
Content 13.1%, copper chelation percent 93.3%, content of peptides 69%.Meanwhile the waste liquid containing free copper ion adds caused by nanofiltration system
Sodium hydrate solid, copper ion is precipitated, be separated by filtration Kocide SD and precipitate and focus on.
The detailed word description it is pointed out that though above-described embodiment is contrasted to the present invention, these words are retouched
State and simply mentality of designing of the present invention is briefly described, rather than the limitation to thinking of the present invention.It is any to be set no more than the present invention
Combination, increase or the modification of thinking are counted, is each fallen within protection scope of the present invention.
Claims (5)
- A kind of 1. method that yeast polypeptides chelated copper is prepared using beer waste yeast, it is characterised in that：Described yeast polypeptides chela The preparation method for closing copper specifically comprises the following steps：Pretreatment：Beer waste yeast liquid carries out broken wall treatment through ultrasonic system, retains supernatant after centrifugation, adds appropriate bicarbonate Sodium carries out de- suffering reason, and pretreatment fluid is made；The extraction of yeast polypeptides：After pretreatment fluid adjusts pH and temperature conditionss, add appropriate protease and carry out enzyme digestion reaction, enzyme deactivation Yeast polypeptides solution is generated afterwards；The chelating of yeast polypeptides and mantoquita：The pH and temperature of yeast polypeptides solution are adjusted, the mantoquita for adding appropriate ratio carries out chela Close reaction；Finished product is dried in removal of impurities：After chelatropic reaction terminates, chelating liquid is simultaneously concentrated by nanofiltration system, removing free copper ion, Through high temperature spray-drying, yeast polypeptides chelated copper powder product is obtained.
- 2. the preparation method of yeast polypeptides chelated copper according to claim 1, it is characterised in that：Step（1）At middle ultrasound Manage bar part is 30-50kHZ, ultrasonic 1-2h, centrifugal condition 4000-6000r/min, centrifuges 15-30min, sodium acid carbonate addition Measure as the 0.5%-1.0% of waste yeast liquid quality, normal-temperature reaction 0.5-2h.
- 3. the preparation method of yeast polypeptides chelated copper according to claim 1, it is characterised in that：Step（2）Middle addition Protease is papain, enzymatic hydrolysis condition：Enzyme addition is the 0.08%-0.15%, pH6-8, temperature 30- of pretreatment fluid quality 40 DEG C, time 12-24h, enzyme deactivation condition is 90 DEG C of temperature, time 30min.
- 4. the preparation method of yeast polypeptides chelated copper according to claim 1, it is characterised in that：Step（3）Middle mantoquita Addition is the 10%-15% of polypeptide solution quality, and chelating condition is：PH6.5-7.5,55-65 DEG C of temperature, time 2-4h, wherein Mantoquita is copper sulphate, the one or two of copper chloride.
- 5. the preparation method of yeast polypeptides chelated copper according to claim 1, it is characterised in that：Step（4）Middle chelating liquid By its solid content control after nanofiltration system, in the range of 40%-50%, spray drying condition is：190 DEG C of inlet temperature- 210 DEG C, 90 DEG C -100 DEG C of outlet temperature, product index is chelating copper content 8-12%, copper chelation percent 88-94%, content of peptides 65- 70%。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109528541A (en) * | 2018-12-18 | 2019-03-29 | 澳宝化妆品（惠州）有限公司 | A kind of cleaning compositions of the polynary yeast complexing polypeptide containing five mines |
| CN110327273A (en) * | 2019-08-06 | 2019-10-15 | 苏格格 | Lightening compositions, Skin whitening care cosmetics and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286105A (en) * | 2011-06-14 | 2011-12-21 | 中国农业大学 | Sesame protein source metal chelating peptide and peptide trace element chelate and application thereof |
| CN106538844A (en) * | 2016-11-26 | 2017-03-29 | 青岛中泰和生物科技有限公司 | A kind of preparation method of marine protein peptide chelated copper |
-
2017
- 2017-06-10 CN CN201710435173.XA patent/CN107410668A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286105A (en) * | 2011-06-14 | 2011-12-21 | 中国农业大学 | Sesame protein source metal chelating peptide and peptide trace element chelate and application thereof |
| CN106538844A (en) * | 2016-11-26 | 2017-03-29 | 青岛中泰和生物科技有限公司 | A kind of preparation method of marine protein peptide chelated copper |
Non-Patent Citations (3)
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| 凌秀梅: "利用啤酒废酵母制备多肽的研究", 《中国中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109528541A (en) * | 2018-12-18 | 2019-03-29 | 澳宝化妆品（惠州）有限公司 | A kind of cleaning compositions of the polynary yeast complexing polypeptide containing five mines |
| CN110327273A (en) * | 2019-08-06 | 2019-10-15 | 苏格格 | Lightening compositions, Skin whitening care cosmetics and preparation method thereof |
| CN110327273B (en) * | 2019-08-06 | 2022-02-15 | 广州名妆化妆品制造有限公司 | Whitening composition, whitening skin care product and preparation method thereof |
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Application publication date: 20171201 |