CN107296151A - A kind of utilization beer waste yeast prepares the chelating ferrous method of yeast polypeptides - Google Patents
A kind of utilization beer waste yeast prepares the chelating ferrous method of yeast polypeptides Download PDFInfo
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- CN107296151A CN107296151A CN201710435174.4A CN201710435174A CN107296151A CN 107296151 A CN107296151 A CN 107296151A CN 201710435174 A CN201710435174 A CN 201710435174A CN 107296151 A CN107296151 A CN 107296151A
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- ferrous
- yeast
- chelating
- temperature
- yeast polypeptides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Inorganic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The chelating ferrous method of yeast polypeptides is prepared the invention provides a kind of utilization beer waste yeast, this method obtains the chelating ferrous powder product of yeast polypeptides by the program such as broken wall, de- hardship, enzymolysis, chelating, removal of impurities, drying.The chelating ferrous content of the chelating ferrous product of yeast polypeptides obtained in this method is high, and ferrous chelation percent is high, and albumen peptide activity is high, and this method makes beer waste yeast turn waste into wealth, and operating procedure is simple, and cost is relatively low, and new approach is opened up to improve its added value.
Description
Technical field
The chelating ferrous method of yeast polypeptides is prepared the present invention relates to a kind of utilization beer waste yeast, belongs to feed addictive
Technical field.
Background technology
Beer waste yeast is the important by-products in Beer Brewage, accounts for the 2%-3% of beer production.Contain in beer waste yeast
There is abundant nutritional ingredient, according to surveying and determination, it contains 45%-60% protein, 25%-35% carbohydrate, 4%-7% fat
Fat, is a kind of good protein resource.Because beer waste yeast protein component is excellent, yield is big, cost is low, therefore it has
Have broad application prospects and good economic and social benefits.At present, it is directed to the recycling of beer waste yeast very in China
Limited, wherein only 40%~50% yeast is recycled, remaining 50%~60% all goes out of use, and causes a large amount of high-quality
The waste of resource.How improving beer waste yeast recovery utilization rate and opening up its higher value application approach is that needs are urgently to be resolved hurrily
The problem of.
The chelate of ferrous ion and polypeptide is a kind of new benefit iron product of in the market, and it has, and excitant is small, absorb
Hurry up, with amino acid uncontested absorption the advantages of, it has also become the research of feed addictive in the market and the focus paid close attention to.From the useless ferment of beer
Female polypeptide extracted carries out the chelating ferrous beer waste yeast that not only solves of chelatropic reaction generation yeast polypeptides with ferrous salt and reclaimed
Utilization rate and higher value application problem, while it is significant also to develop green animal husbandry to China.Had no at present through the retrieval country
The chelating ferrous relevant report of yeast polypeptides is prepared using beer waste yeast.
How a kind of utilization beer waste yeast is provided and prepares the chelating ferrous preparation method of yeast polypeptides, and this method is simple
Easy, cost is low, can make full use of beer waste yeast resource, is the problem of present invention needs solution.
The content of the invention
Based on above mentioned problem, the chelating ferrous side of yeast polypeptides is prepared the invention provides a kind of utilization beer waste yeast
Method, the preparation technology is to chelate that to obtain yeast polypeptides chelating ferrous by extracting polypeptide and ferrous salt from beer waste yeast.
The concrete technical scheme that the present invention is used is as follows：
A kind of utilization beer waste yeast prepares the chelating ferrous method of yeast polypeptides, in turn includes the following steps：
（1）Broken wall：By beer waste yeast liquid by ultrasonically treated, centrifugation reservation supernatant；
（2）De- hardship：Appropriate sodium acid carbonate is added in supernatant and carries out de- suffering reason, pretreatment fluid is made；
（3）Enzymolysis：Pretreatment fluid pH value and temperature are adjusted, adds after protease carries out enzyme digestion reaction, destroy the enzyme treatment and yeast is made
Polypeptide solution；
（4）Chelating：Yeast polypeptides solution ph and temperature are adjusted, ferrous salt is added and antioxidant carries out chelatropic reaction；
（5）Removal of impurities：Concentrated by nanofiltration system and remove free ferrous iron；
（6）Dry：Concentrate high temperature spray-drying obtains the chelating ferrous powder product of yeast polypeptides.
The chelating ferrous preparation method of yeast polypeptides described above, the step（1）In ultrasonically treated condition be 30-
50kHZ, ultrasonic 1-2h, centrifugal condition is 4000-6000r/min, centrifuges 15-30min.
The chelating ferrous preparation method of yeast polypeptides described above, the step（2）Middle sodium acid carbonate addition is useless ferment
The 0.5%-1.0% of mother liquor quality, normal-temperature reaction 0.5-2h.
The chelating ferrous preparation method of yeast polypeptides described above, the step（3）The protease of middle addition is pawpaw egg
White enzyme, enzymatic hydrolysis condition：Enzyme addition is 0.08%-0.15%, the pH6-8 of pretreatment fluid quality, 30-40 DEG C of temperature, time 12-
24h, enzyme condition of going out is 90 DEG C of temperature, time 30min.
The chelating ferrous preparation method of yeast polypeptides described above, the step（4）Middle ferrous salt addition is that polypeptide is molten
The 15-20% of liquid quality, antioxidant addition is the 20% of ferrous salt quality, and chelating condition is：PH6.5-7.5, temperature 25-35
DEG C, time 30-60min, wherein ferrous salt be ferrous sulfate, the one or two of frerrous chloride, antioxidant be ascorbic acid,
One or more in sodium isoascorbate, sodium hydrogensulfite, hydroxylamine hydrochloride.
The chelating ferrous preparation method of yeast polypeptides described above, the step（5）After middle chelating liquid is by nanofiltration system
Its solid content is controlled in the range of 40%-50%.
The chelating ferrous preparation method of yeast polypeptides described above, the step（6）Middle spray drying condition is：Import temperature
190 DEG C -210 DEG C of degree, 90 DEG C -100 DEG C of outlet temperature；Product index is：Chelating ferrous content 10%-14%, ferrous chelation percent
95%-98%, ferric iron content 0%, content of peptides 50-60%.
The technology of the present invention has following technical advantage：Using protease method degraded Yeast protein, gained albumen in the present invention
Peptides extraction rate is high, and ferrous chelation percent is high during chelating, and whole preparation process operating procedure is simple, exploitativeness strong, cost
It is relatively low, the real higher value application realized to beer waste yeast.
Embodiment
The present invention is further described below by way of specific embodiment, but described embodiment does not limit this in any way
The scope of patent protection of invention.
Embodiment 1：Beer waste yeast liquid 1000kg is pumped at ultrasonic treatment systems, 40kHZ ultrasonication 1.0h, ultrasound
30min is centrifuged through 4000r/min supercentrifuges after reason, supernatant is pumped into retort, add waste yeast liquid quality 0.5%
Sodium acid carbonate, opens stirring system, normal-temperature reaction 1.5h.Regulation pretreatment fluid pH value adds pretreatment to 7.0 after reaction terminates
The papain of liquid quality 0.1%, opens heating and stirring system, is warming up to 30 DEG C and is incubated, enzyme digestion reaction 24h.Reaction knot
Shu Hou, is warming up to 90 DEG C, and go out enzyme 30min, obtains yeast polypeptides solution.Polypeptide solution temperature is down to 30 DEG C, holding pH is
7.0, add the ferrous sulfate of polypeptide solution quality 15%（Seven water）Ascorbic acid quality is ferrous sulfate（Seven water）20%, chelating
Reaction time 30min, is made the chelating ferrous solution of yeast polypeptides.Solution filters system by sodium, remove it is a small amount of it is free it is ferrous from
Son is simultaneously concentrated into solid content 45%.Concentrate obtains yeast through high temperature spray-drying, 200 DEG C of inlet temperature, 90 DEG C of outlet temperature
Polypeptide chelate ferrous iron pulvis, index is：Chelating ferrous content 13.5%, ferrous chelation percent 97.5%, ferric iron content 0%, polypeptide contains
Amount 65%.
Embodiment 2：Beer waste yeast liquid 5000kg is pumped at ultrasonic treatment systems, 30kHZ ultrasonication 2.0h, ultrasound
15min is centrifuged through 6000r/min supercentrifuges after reason, supernatant is pumped into retort, add waste yeast liquid quality 1.0%
Sodium acid carbonate, opens stirring system, normal-temperature reaction 0.5h.Regulation pretreatment fluid pH value adds pretreatment to 7.0 after reaction terminates
The papain of liquid quality 0.15%, opens heating and stirring system, is warming up to 30 DEG C and is incubated, enzyme digestion reaction 24h.Reaction
After end, 90 DEG C are warming up to, go out enzyme 30min, obtains yeast polypeptides solution.Polypeptide solution temperature is down to 35 DEG C, holding pH is
7.0, add the ferrous sulfate of polypeptide solution quality 20%（Seven water）Ascorbic acid quality is ferrous sulfate（Seven water）20%, chelating
Reaction time 40min, is made the chelating ferrous solution of yeast polypeptides.Solution filters system by sodium, remove it is a small amount of it is free it is ferrous from
Son is simultaneously concentrated into solid content 48%.Concentrate obtains yeast through high temperature spray-drying, 200 DEG C of inlet temperature, 90 DEG C of outlet temperature
Polypeptide chelate ferrous iron pulvis, index is：Chelating ferrous content 13.8%, ferrous chelation percent 98.2%, ferric iron content 0%, polypeptide contains
Amount 67%.
The detailed word description it is pointed out that though above-described embodiment is contrasted to the present invention, these words are retouched
State and simply mentality of designing of the present invention is briefly described, rather than the limitation to thinking of the present invention.It is any to be set no more than the present invention
The combination, increase or modification of thinking are counted, is each fallen within protection scope of the present invention.
Claims (7)
1. a kind of ferrous method of utilization beer waste yeast polypeptide chelate, it is characterised in that：Described yeast polypeptides are chelating ferrous
Preparation method specifically include following steps：
（1）Broken wall：By beer waste yeast liquid by ultrasonically treated, centrifugation reservation supernatant；
（2）De- hardship：Appropriate sodium acid carbonate is added in supernatant and carries out de- suffering reason, pretreatment fluid is made；
（3）Enzymolysis：Pretreatment fluid pH value and temperature are adjusted, adds after protease carries out enzyme digestion reaction, destroy the enzyme treatment and yeast is made
Polypeptide solution；
（4）Chelating：Yeast polypeptides solution ph and temperature are adjusted, ferrous salt is added and antioxidant carries out chelatropic reaction；
（5）Removal of impurities：Concentrated by nanofiltration system and remove free ferrous iron；
（6）Dry：Concentrate high temperature spray-drying obtains the chelating ferrous powder product of yeast polypeptides.
2. the chelating ferrous preparation method of yeast polypeptides according to claim 1, it is characterised in that：Step（1）Middle ultrasound
Treatment conditions are 30-50kHZ, and ultrasonic 1-2h, centrifugal condition is 4000-6000r/min, centrifuge 15-30min.
3. the chelating ferrous preparation method of yeast polypeptides according to claim 1, it is characterised in that：Step（2）Middle carbonic acid
Hydrogen sodium addition is the 0.5%-1.0%, normal-temperature reaction 0.5-2h of waste yeast liquid quality.
4. the chelating ferrous preparation method of yeast polypeptides according to claim 1, it is characterised in that：Step（3）Middle addition
Protease be papain, enzymatic hydrolysis condition：Enzyme addition is the 0.08%-0.15%, pH6-8, temperature of pretreatment fluid quality
30-40 DEG C, time 12-24h, enzyme condition of going out is 90 DEG C of temperature, time 30min.
5. the chelating ferrous preparation method of yeast polypeptides according to claim 1, it is characterised in that：Step（4）Middle ferrous iron
Salt addition is the 15-20% of polypeptide solution quality, and antioxidant addition is the 20% of ferrous salt quality, and chelating condition is：
PH6.5-7.5,25-35 DEG C of temperature, time 30-60min, wherein ferrous salt be ferrous sulfate, the one or two of frerrous chloride,
Antioxidant is the one or more in ascorbic acid, sodium isoascorbate, sodium hydrogensulfite, hydroxylamine hydrochloride.
6. the chelating ferrous preparation method of yeast polypeptides according to claim 1, it is characterised in that：Step（5）Middle chelating
Liquid is by its solid content control after nanofiltration system in the range of 40%-50%.
7. the chelating ferrous preparation method of yeast polypeptides according to claim 1, it is characterised in that：Step（6）Middle spraying
Drying condition is：190 DEG C -210 DEG C of inlet temperature, 90 DEG C -100 DEG C of outlet temperature；Product index is：Chelating ferrous content 10%-
14%, ferrous chelation percent 95%-98%, ferric iron content 0%, content of peptides 50-60%.
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| CN201710435174.4A CN107296151A (en) | 2017-06-10 | 2017-06-10 | A kind of utilization beer waste yeast prepares the chelating ferrous method of yeast polypeptides |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794260A (en) * | 2018-06-29 | 2018-11-13 | 陕西省蒲城绿桥农化有限责任公司 | A method of fulvic acid chelated iron fertilizer is prepared using solid-phase grinding technology |
| CN109528541A (en) * | 2018-12-18 | 2019-03-29 | 澳宝化妆品（惠州）有限公司 | A kind of cleaning compositions of the polynary yeast complexing polypeptide containing five mines |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286105A (en) * | 2011-06-14 | 2011-12-21 | 中国农业大学 | Sesame protein source metal chelating peptide and peptide trace element chelate and application thereof |
| CN106538845A (en) * | 2016-11-26 | 2017-03-29 | 青岛中泰和生物科技有限公司 | A kind of chelating ferrous preparation method of marine protein peptide |
-
2017
- 2017-06-10 CN CN201710435174.4A patent/CN107296151A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286105A (en) * | 2011-06-14 | 2011-12-21 | 中国农业大学 | Sesame protein source metal chelating peptide and peptide trace element chelate and application thereof |
| CN106538845A (en) * | 2016-11-26 | 2017-03-29 | 青岛中泰和生物科技有限公司 | A kind of chelating ferrous preparation method of marine protein peptide |
Non-Patent Citations (3)
| Title |
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| 凌秀梅: "利用啤酒废酵母制备多肽的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 张莉弘等: "几种废啤酒酵母细胞破壁方法的比较", 《长春大学学报》 * |
| 谢蓝华等: "蛋壳粉制备酵母多肽螯合钙", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794260A (en) * | 2018-06-29 | 2018-11-13 | 陕西省蒲城绿桥农化有限责任公司 | A method of fulvic acid chelated iron fertilizer is prepared using solid-phase grinding technology |
| CN109528541A (en) * | 2018-12-18 | 2019-03-29 | 澳宝化妆品（惠州）有限公司 | A kind of cleaning compositions of the polynary yeast complexing polypeptide containing five mines |
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Application publication date: 20171027 |
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