CN107410025A - A kind of method for cultivating oil tea sterile bud - Google Patents
A kind of method for cultivating oil tea sterile bud Download PDFInfo
- Publication number
- CN107410025A CN107410025A CN201710480896.1A CN201710480896A CN107410025A CN 107410025 A CN107410025 A CN 107410025A CN 201710480896 A CN201710480896 A CN 201710480896A CN 107410025 A CN107410025 A CN 107410025A
- Authority
- CN
- China
- Prior art keywords
- oil tea
- benevolence
- sterile bud
- culture
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method for cultivating oil tea sterile bud, concretely comprise the following steps：Tea oil tree seed is removed into kind of a skin, sterilized water soaks 6 8h；Then priority alcohol and HgCl2Sterilization, aseptic water washing；Benevolence inoculation is contained on the culture medium of multitudinous sugar, agar, the basic element of cell division, the acetic acid of indoles 3, gibberellin, antibacterial peptide, is gone to after dark training under illumination condition and cultivates 28 32d, produce oil tea sterile bud.Have the beneficial effect that：Sterile bud success rate obtained by the culture is high, short time can provide a large amount of seedling for oil tea, with good practicality, a new technological approaches is provided for quick breeding oil tea plant, and for by the method improvement oil tea resistance of genetic engineering, cultivation disease-resistant plant, the foundation for improving tea-oil tree yield and oil tea genetic system lays the first stone later.
Description
Technical field
The present invention relates to technical field of plant culture, more particularly, to a kind of method for cultivating oil tea sterile bud.
Background technology
Oil tea is Theaceae Camellia Plants, also known as the young tree of tea oil tree, tea, is the distinctive woody edible oil material of south China
Seeds, it is cured 2300 using cultivation history.Oil tea and olive are pulled, oily track, move son and referred to as big woody edible oil source seeds in the world four,
Tea oil unsaturated fatty acid content is up to more than 90, and color is good savory, and unique flavor is nutritious, and resistance to courage is hidden, and has hypotensive, blood fat
And the effect of softening blood vessel, the prevention to also vascular diseases play an important role, are one of high-quality edible oils, are described as " east
Olive pulls oil ".Meanwhile oil tea accessory substance still produces the quality raw materials of fertilizer, agricultural chemicals, mimosa extract.Oil tea is distributed mainly on the Qin of China
On the south ridge, Huaihe River, wherein Hunan, Jiangxi, Guangxi, Zhejiang, Fujian etc. is main producing region, the kind of Main Cultivation have camellia meiocarpa,
Stains camellias, Camellia yuhsienensis, C.chekiangoleosa, Vietnam's black tea etc..According to statistics, camellia oleifera lam cultivated area in China's is up to 60,000,000 mu,
Cultivate huge, but some areas management is extensive, cause camellia oleiferaindustry in the prevalence of yield per unit area it is low and
Deficiency in economic performance two large problems.One of poorly efficient major reason of oil tea low yield is exactly that improved variety degree is low, and kind is chaotic, most of
Camellia oleifera lam is in wild state, and yield is relatively low, and the shortage of breeding nursery stock can not be widely applied plantation.Meanwhile Oil Tea Anthracnose is
The Major Diseases of oil tea, the large area oil tea cultivation area of each province on the south the Yangtze river basin, and Henan, South Shaanxi occur
Generally.After disease occurs, cause serious shedding, bud drop, branch withered, or even whole strain decline, have a strong impact on camellia oleiferaindustry
Develop in a healthy way.Camellia oleifera lam improvement needs a large amount of high quality seedling supplies, and the cuttage graft seedling growth production time is long, is limited by field season etc.
System, difficult W meet production needs.The oil tea tissue culture regeneration of efficient stable is established by plant tissue culture technique, carries out oil
Tea choiceness breed with kind genetic improvement, there are important research and production to be worth.
Prior art such as Authorization Notice No. is the B of CN 102696480 Chinese invention patent, discloses husky matrix in a kind of test tube
The method for cultivating oil tea sterile bud, test tube is loaded by 1/4 charge weight using the river sand of 80~100 mesh fresh cleans, addition contains
The MS fluid nutrient mediums of 0.01~0.02g/L ascorbic acid are used as culture matrix to mixing is moistened, and 1~5 year oil generation tea age was planted
The spray of semi-lignified or firm lignifying of the strain tree crown periphery middle and upper part with axillary bud cut into band axillary bud 1~2, it is long by 3.0~
4.0cm segment, sterilized cuttage are cultivated into culture matrix, and the tender shoots that clip is newly sprouted, which is forwarded in MS culture mediums, to continue to train
Support and obtain oil tea sterile bud.This method culture oil tea sterile bud success rate is high, good in economic efficiency, but cultivation cycle is grown, in short-term
Interior is difficult to provide a large amount of oil tea seedling.
The content of the invention
High it is an object of the invention to provide a kind of sterile bud success rate, cultivation cycle is short, and practical bean sprouts is without soil
Nutrient solution.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is：A kind of culture oil tea sterile bud
Method, including benevolence prepares, benevolence sterilizes, prepared by culture medium, benevolence culture, concretely comprises the following steps：
Benevolence prepares：The seed of the full fruit on excellent tea oil tree is selected, removes kind of a skin, the kind for select no mould, damaging by worms
Benevolence, rinsed well with water, sterilized water immersion 6-8h, standby, tea seed has dormant trait, after eliminating the hard exosper of oil tea
Immersion can break the dormancy of Seed of Camellia oleifera, promote seed to sprout, and improve germination percentage；
Benevolence sterilizes：Benevolence after immersion first used to 70-80% alcohol disinfecting 30-40s, aseptic water washing 4-6 times, then with dense
Spend the HgCl for 0.8-1.2%25-7min is sterilized, aseptic water washing, cuts away the major part of endosperm, standby, the step is to control
Liquefaction tea benevolence pollutes, and obtains the sterilizable material of abundance, and this is the key for obtaining oil tea sterile bud, and oil tea embryo is smaller, cuts
Embryo will examine when being inoculated with, and should carry less endosperm can not cut cur's embryo again；
It is prepared by culture medium：Take multitudinous sugared 28-32 parts of parts by weight, agar 5-8 parts, basic element of cell division 0.002-0.004 parts, indoles-
3- acetic acid 0.00005-0.00008 parts, gibberellin 0.001-0.002 parts, antibacterial peptide 0.002-0.003 parts, water 1000-1100
Part, it is well mixed, regulation pH value is 5-6, sterilizing, produces culture medium, the amino acid sequence of antibacterial peptide is
HSHACASYSRYHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the antibacterial peptide have broad spectrum antibiotic activity, have to bacterium very strong
Lethal effect, can act on bacterial cell membrane, on film formed cross-film ion channel, destroy the integrality of film, make
Into cellular content leak, so as to kill bacterial cell, prevent culture medium to be infected by bacterial, for culture sterile bud provide one it is good
Good growing environment, the culture medium can provide sufficient nutriment for the germination of benevolence, shorten sprout time, improve germination
Rate, oil tea benevolence speed of germination and germination regularity can be accelerated, and the generation of bacterial components can be suppressed, reduce the pollution of culture
Rate so that culture oil tea sterile bud success rate is high；
Benevolence culture：By on the benevolence inoculation medium after processing, gone to after light culture 45-48h under illumination condition and cultivate 28-
32d, temperature are 25-27 DEG C, and intensity of illumination is 2000-2200 μm of olm-2·s-1Light application time is 13-15h/d, produces oil tea
Sterile bud, the sterile bud success rate obtained by the culture is high, and the short time can provide a large amount of seedling for oil tea, has practical well
Property, a new technological approaches is provided for quick breeding oil tea plant, and to pass through the method improvement of genetic engineering later
Oil tea resistance, disease-resistant plant is cultivated, the foundation for improving tea-oil tree yield and oil tea genetic system lays the first stone.
Compared with prior art, the advantage of the invention is that：1）The culture medium of the present invention can provide for the germination of benevolence to be filled
The nutriment divided, shorten sprout time, improve germination percentage, oil tea benevolence speed of germination and germination regularity, and energy can be accelerated
Suppress the generation of bacterial components, reduce the pollution rate of culture；2）Sterile bud success rate obtained by cultural method is high, and the short time can be with
A large amount of seedling are provided for oil tea, there is good practicality, a new technological approaches is provided for quick breeding oil tea plant,
And by the method improvement oil tea resistance of genetic engineering, cultivation disease-resistant plant, to improve tea-oil tree yield and oil tea heredity later
The foundation of system lays the first stone；3）Cultural method simple possible, technology are easily received and grasped, and have large development space.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of method for cultivating oil tea sterile bud, including benevolence prepares, benevolence sterilizes, prepared by culture medium, benevolence culture, specific step
Suddenly it is：
1）Benevolence prepares：The seed of the full fruit on excellent tea oil tree is selected, removes kind of a skin, no mould is selected, damages by worms
Benevolence, rinsed well with water, sterilized water immersion 7h, standby, tea seed has dormant trait, after eliminating the hard exosper of oil tea
Immersion can break the dormancy of Seed of Camellia oleifera, promote seed to sprout, and improve germination percentage；
2）Benevolence sterilizes：By the benevolence after immersion first with 78% alcohol disinfecting s, aseptic water washing 5 times, then with concentration be 1%
HgCl26min is sterilized, aseptic water washing 5 times, cuts away the major part of endosperm, standby, the step is to control oil tea benevolence dirty
Dye, the sterilizable material of abundance is obtained, this is the key for obtaining oil tea sterile bud, and oil tea embryo is smaller, is wanted when cutting embryo inoculation
Examine, should carry less endosperm can not cut cur's embryo again；
3）It is prepared by culture medium：Take multitudinous sugared 30 parts of parts by weight, 7 parts of agar, 0.003 part of the basic element of cell division, indole-3-acetic acid
0.00006 part, 0.001 part of gibberellin, 0.002 part of antibacterial peptide, 1000 parts of water, be well mixed, regulation pH value be 5.5, sterilizing, i.e.,
Culture medium, the amino acid sequence of antibacterial peptide are HSHACASYSRYHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the antibacterial peptide
With broad spectrum antibiotic activity, there is very strong lethal effect to bacterium, can act on bacterial cell membrane, cross-film is formed on film
Ion channel, the integrality of film is destroyed, causes cellular content to leak, so as to kill bacterial cell, prevent that culture medium is thin
Bacterium infects, and provides a good growing environment for culture sterile bud, the culture medium can provide sufficient battalion for the germination of benevolence
Material is supported, shortens sprout time, germination percentage is improved, oil tea benevolence speed of germination and germination regularity can be accelerated, and can suppress thin
The generation of fungus matter, reduce the pollution rate of culture so that culture oil tea sterile bud success rate is high；
4）Benevolence culture：By on the benevolence inoculation medium after processing, gone to after light culture 48h under illumination condition and cultivate 30d, temperature
Spend for 26 DEG C, intensity of illumination is 2100 μm of olm-2·s-1, light application time 14h/d, oil tea sterile bud is produced, the culture institute
The sterile bud success rate obtained is high, and the short time can provide a large amount of seedling for oil tea, have good practicality, oily for quick breeding
Tea plant provides a new technological approaches, and to be resisted by the method improvement oil tea resistance of genetic engineering, cultivation later
Sick plant, the foundation for improving tea-oil tree yield and oil tea genetic system lay the first stone.
Embodiment 2：
A kind of method for cultivating oil tea sterile bud, is concretely comprised the following steps：
1）The seed of the full fruit on excellent tea oil tree is selected, removes kind of a skin, the benevolence for select no mould, damaging by worms, uses water
Rinse well, sterilized water immersion 6h, standby, tea seed has dormant trait, soaks and can beat after eliminating the hard exosper of oil tea
The dormancy of broken Seed of Camellia oleifera, promotes seed to sprout, and improves germination percentage；
2）By the benevolence after immersion first with 75% alcohol disinfecting 32s, aseptic water washing, then with concentration be 1.2% HgCl2Sterilization
5min, aseptic water washing, the major part of endosperm is cut away, standby, the step is to control oil tea benevolence to pollute, and obtains abundance
Sterilizable material, this is the key for obtaining oil tea sterile bud, and oil tea embryo is smaller, to be examined when cutting embryo inoculation, should
Cur's embryo can not be cut again with less endosperm；
3）Benevolence after processing is inoculated on the culture medium that pH value is 5.5, the composition and its parts by weight of culture medium are：Multitudinous sugared 31
Part, 7 parts of agar, 0.004 part of the basic element of cell division, 0.00008 part of indole-3-acetic acid, 0.0015 part of gibberellin, antibacterial peptide 0.0022
Part, 1000 parts of water, go under illumination condition after light culture 46h and cultivate 32d, and temperature is 25 DEG C, and intensity of illumination is 2200 μ
mol·m-2·s-1, light application time 13h/d, oil tea sterile bud is produced, the sterile bud success rate obtained by the culture is high, the short time
A large amount of seedling can be provided for oil tea, there is good practicality, a new technology is provided for quick breeding oil tea plant
Approach, and by the method improvement oil tea resistance of genetic engineering, cultivation disease-resistant plant, to improve tea-oil tree yield and oil tea later
The foundation of genetic system lays the first stone.
Embodiment 3：
A kind of method for cultivating oil tea sterile bud, is concretely comprised the following steps：The seed of the full fruit on excellent tea oil tree is selected, is removed
Kind skin, the benevolence for select no mould, damaging by worms, is rinsed well with water, and sterilized water soaks 8h, standby；Benevolence after immersion is first
With 74% alcohol disinfecting 33s, with the HgCl that concentration is 0.9% after aseptic water washing25min is sterilized, embryo is cut away after aseptic water washing
The major part of breast；Take multitudinous sugared 32 parts of parts by weight, 7 parts of agar, 0.004 part of the basic element of cell division, 0.00007 part of indole-3-acetic acid,
0.0016 part of gibberellin, 0.002 part of antibacterial peptide, 1100 parts of water, it is well mixed, regulation pH value is 5-6, sterilizing, produces culture medium；
By on the benevolence inoculation medium after processing, gone to after light culture 47h under illumination condition and cultivate 31d, temperature is 26 DEG C, and illumination is strong
Spend for 2200 μm of olm-2·s-1, light application time 14h/d, produce oil tea sterile bud.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City is difficult to understand and special agricultural science and technology Co., Ltd
<120>A kind of method for cultivating oil tea sterile bud
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 39
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Ser Tyr Ser Arg Tyr His Arg Gly Arg Cys
1 5 10 15
Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His Pro Gly Lys Leu Cys
20 25 30
Val Cys Val Asn Cys Ser Arg
35
Claims (10)
1. a kind of method for cultivating oil tea sterile bud, including benevolence prepares, benevolence sterilizes, prepared by culture medium, benevolence culture, it is special
Sign is：The composition of culture medium and its parts by weight are in prepared by described culture medium：Multitudinous sugared 28-32 parts, agar 5-8 parts, cell
Mitogen 0.002-0.004 parts, indole-3-acetic acid 0.00005-0.00008 parts, gibberellin 0.001-0.002 parts, antibacterial peptide
0.002-0.003 parts, water 1000-1100 parts.
A kind of 2. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：It is prepared by described culture medium
The amino acid sequence of antibacterial peptide is HSHACASYSRYHRGRCYCRCLRCRVLHPGKLCVCVNCSR in middle culture medium.
A kind of 3. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：It is prepared by described culture medium
The pH value of middle culture medium is 5-6.
A kind of 4. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：During described benevolence prepares
Benevolence soaks 6-8h with sterilized water in benevolence sterilization.
A kind of 5. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：Described benevolence sterilization
For：Aseptic water washing will be used after benevolence alcohol disinfecting after immersion, then use HgCl2Sterilization, then with aseptic water washing, cuts away embryo
The major part of breast, it is standby.
A kind of 6. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：In described benevolence sterilization
Alcohol concentration is 70-80%, sterilizes 30-40s.
A kind of 7. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：In described benevolence sterilization
HgCl2Concentration be 0.8-1.2%, sterilize 5-7min.
A kind of 8. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：In described benevolence culture
Light culture 45-48h.
A kind of 9. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：In described benevolence culture
28-32d is cultivated under illumination condition.
A kind of 10. method for cultivating oil tea sterile bud according to claim 1, it is characterised in that：Described benevolence culture
Middle illumination cultivation temperature is 25-27 DEG C, and intensity of illumination is 2000-2200 μm of olm-2·s-1, light application time 13-15h/d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710480896.1A CN107410025A (en) | 2017-06-22 | 2017-06-22 | A kind of method for cultivating oil tea sterile bud |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710480896.1A CN107410025A (en) | 2017-06-22 | 2017-06-22 | A kind of method for cultivating oil tea sterile bud |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107410025A true CN107410025A (en) | 2017-12-01 |
Family
ID=60426687
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710480896.1A Pending CN107410025A (en) | 2017-06-22 | 2017-06-22 | A kind of method for cultivating oil tea sterile bud |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107410025A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558742A (en) * | 2009-05-12 | 2009-10-21 | 中国林业科学研究院亚热带林业研究所 | Method for regenerating plant from camellia callus |
| CN103081807A (en) * | 2013-02-01 | 2013-05-08 | 中国林业科学研究院亚热带林业研究所 | Method for regenerating plant by use of callus of camellia japonica |
| CN104823861A (en) * | 2015-06-08 | 2015-08-12 | 中南林业科技大学 | Method for acquiring regeneration plants by adventitious bud inducement of camellia oleifera radicles |
| CN104885948A (en) * | 2015-06-08 | 2015-09-09 | 中南林业科技大学 | Method for directly regenerating plants by tea-oil tree cotyledonary nodes |
| CN104938335A (en) * | 2015-06-08 | 2015-09-30 | 中南林业科技大学 | Method for obtaining regeneration plants by use of camellia oleifera hypocotyl |
-
2017
- 2017-06-22 CN CN201710480896.1A patent/CN107410025A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558742A (en) * | 2009-05-12 | 2009-10-21 | 中国林业科学研究院亚热带林业研究所 | Method for regenerating plant from camellia callus |
| CN103081807A (en) * | 2013-02-01 | 2013-05-08 | 中国林业科学研究院亚热带林业研究所 | Method for regenerating plant by use of callus of camellia japonica |
| CN104823861A (en) * | 2015-06-08 | 2015-08-12 | 中南林业科技大学 | Method for acquiring regeneration plants by adventitious bud inducement of camellia oleifera radicles |
| CN104885948A (en) * | 2015-06-08 | 2015-09-09 | 中南林业科技大学 | Method for directly regenerating plants by tea-oil tree cotyledonary nodes |
| CN104938335A (en) * | 2015-06-08 | 2015-09-30 | 中南林业科技大学 | Method for obtaining regeneration plants by use of camellia oleifera hypocotyl |
Non-Patent Citations (4)
| Title |
|---|
| 唐国涛 等: "油茶组织培养繁殖技术初步研究", 《广东林业科技》 * |
| 王蒂 等: "《植物组织培养》", 31 August 2013, 中国农业出版社 * |
| 郎跃深: "《蝇蛆养殖关键技术与应用》", 31 May 2015, 科学技术文献出版社 * |
| 郭兆武: "《景观植物学》", 31 March 2014, 中国农业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104719165B (en) | A kind of quickly tissue culture method of black fruit lyceum | |
| CN105340747A (en) | Asexual rapid propagation method for radix glycyrrhizae | |
| CN101940158A (en) | Method for rapidly propagating plantlets by utilizing bletilla striata seeds | |
| CN105420125A (en) | Arbuscular mycorrhizal fungal inoculant | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN109258460A (en) | Micro-stem tip culture combines the breeding method of heat treatment acquisition Zengcheng honey chrysanthemum detoxic seedling | |
| CN104756869A (en) | Method for preparation of pinus massoniana lamb superior individual sterile explant and induction of initial bud | |
| CN105309311A (en) | Method for breeding improved variety of scrophularia ningpoensis Hemsl. | |
| CN103125393B (en) | Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am | |
| CN103070014B (en) | Method for breeding summer truffle root seedling through inoculation of suspension liquid | |
| CN102696482B (en) | Method for detoxifying clonal tissue culture explant of Camellia oleifera Abel | |
| CN105941154A (en) | Comprehensive breeding method for superior winter jujube seedlings | |
| CN103039362B (en) | Subculture medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN103039363B (en) | Rooting medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN102613087A (en) | Method for culturing and breeding Correa carmen by using biological tissue | |
| CN105075860B (en) | A kind of fleabane flower tissue culture method | |
| CN106386499A (en) | Tissue cultured rapid propagation method for Rhizoma Stahlianthi Involucrati | |
| CN106359090A (en) | Method for culturing and planting organic Dendrobium officinale | |
| CN109874676A (en) | A kind of fast breeding method of passion fruit detoxic seedling | |
| CN107372107A (en) | The fast culture process of ground loquat | |
| CN107410025A (en) | A kind of method for cultivating oil tea sterile bud | |
| CN106922515A (en) | A kind of indoor fast breeding method of waste vetch | |
| CN106234229A (en) | Method for temporarily planting taro tissue culture seedlings | |
| CN105794650A (en) | Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds | |
| CN104170738A (en) | Rapid propagation method of acer ginnala maxim. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171201 |
|
| RJ01 | Rejection of invention patent application after publication |