CN107382553A - A kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer - Google Patents

A kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer Download PDF

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Publication number
CN107382553A
CN107382553A CN201710827568.4A CN201710827568A CN107382553A CN 107382553 A CN107382553 A CN 107382553A CN 201710827568 A CN201710827568 A CN 201710827568A CN 107382553 A CN107382553 A CN 107382553A
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Prior art keywords
bacillus subtilis
trichoderma harzianum
parts
marine alga
tunning
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解晓燕
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Hainan Jin Yufeng Biotechnology Co Ltd
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Hainan Jin Yufeng Biotechnology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

Abstract

The invention discloses a kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer.The biological compound fertilizer includes Trichoderma harzianum tunning, bacillus subtilis (Ehrenberg) Cohn fermented product, marine alga.Composite fertilizer prepared by the present invention, using Trichoderma harzianum tunning, bacillus subtilis (Ehrenberg) Cohn fermented product, the compound technology of marine alga, after, crop yield can be increased, increase crop salt resistance alkali, drought resisting, the ability of anti-subterranean pest-insect, while promotion crop increases income, improved soil.

Description

A kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer
Technical field
The present invention relates to a kind of bio-fertilizer, more particularly to a kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer and Its preparation method and application.
Background technology
The mycelia of Trichoderma harzianum is very thin colourless, and tool separates, multi-branched.Conidiophore is born from the side shoot of mycelia, right Raw or alternate, typically has 2-3 branch, sporogenic stigma doleiform estranged or taper.Conidium is mostly spherical, sporoderm tool Pustule, blue-green.At the beginning of bacterium colony it is white cotton fiber shape in PDA culture medium, is afterwards dirty-green.Trichoderma harzianum is agriculturally being widely used as Plant growth regulator, there is inducing plant resistance, start the function of plant defense response.Trichoderma harzianum is agriculturally mainly being adopted It is less using its tunning by the use of its microbial inoculum as plant modifying agent, do not study its tunning pair to different materials yet In the influence of plant growth.
Bacillus subtilis, it is one kind of bacillus.Without pod membrane, peritrichous, can move.Gram-positive bacteria, Ellipse arrives column, and central or slightly inclined positioned at thalline, thalline does not expand after sporulation.Bacterium colony rough surface is opaque, dirty white Or it is slightly yellow, when being grown in liquid medium within, wrinkle mould, aerobic bacteria is commonly formed.The effect of bacillus subtilis, is varied, makees It is widely used for soil conditioner and agent and food additives, using fermentate, as the research of fertilizer, there is presently no report Road.
Marine alga is submarine algae, is the cryptogam of plant kingdom, and algae includes several inhomogeneities with photosynthetic work With the biology for producing energy.They are generally considered to be simple plant, are mainly characterized by:Without vascular tissue, without real Root, stem, the differentiating phenomenon of leaf;Do not bloom, no fruit and seed;Protective tissue of the reproductive organs without specialization, often directly by single Cell produces spore or gamete;And the formation without embryo.
At present, also not on marine alga and Trichoderma harzianum and the compound report for preparing fertilizer of bacillus subtilis.
The content of the invention
The present invention is to overcome above-mentioned defect in the prior art, pass through Trichoderma harzianum tunning, bacillus subtilis Tunning, marine alga is compound to prepare bio-fertilizer, increases crop yield, improves the resistance of crop.
A kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, including Trichoderma harzianum tunning, bacillus subtilis Bacterium tunning, marine alga.
Preferable marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, include the material of following parts by weight:Breathe out thatch wood Mould tunning 30-60 parts, bacillus subtilis (Ehrenberg) Cohn fermented product 30-60 parts, marine alga 300-500 parts.
The marine alga is sargassum, yellow tang, green alga, black algae, sargassum fusifome, the one or more in Sargassum.
The aqua or pulvis of any of the above-described marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer.
The preparation method of above-mentioned marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, is carried out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By string, mineral matter is with water according to mass ratio 100:(1-5):(40- 80) mix, access Trichoderma harzianum fermenting agent, ferment 10-20 days, dry, clay into power, Trichoderma harzianum tunning is made;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:(10-30):(40- 80) mix, access fermentation of bacillus subtilis microbial inoculum, ferment 15-30 days, dry, clay into power, bacillus subtilis hair is made Ferment product;
(3) according to parts by weight, Trichoderma harzianum tunning 30-60 parts, bacillus subtilis (Ehrenberg) Cohn fermented product 30-60 are taken Part, marine alga 300-500 parts, it is uniformly mixed, is made.
The string is bark fiber, soybean fiber, sisal fiber, ramee, oil flax fibre, abaca fibre, One or more in hemp.
The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 3-8X108cfu/g。
The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum is 3-8X108cfu/g。
Above-mentioned marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer is degeneration-resistant in crop yield, it is pest-resistant in application.
Compared with prior art, the present invention has the advantages that:Biological compound fertilizer prepared by the present invention, using Kazakhstan thatch Trichoderma tunning, bacillus subtilis (Ehrenberg) Cohn fermented product, the compound technology of marine alga, after, crop yield can be increased, increase is made Thing salt resistance alkali, drought resisting, the ability of anti-subterranean pest-insect, while promotion crop increases income, improved soil.
Embodiment
The embodiment of the present invention is described in detail below, it is to be understood that protection scope of the present invention is not Limited by embodiment.
Embodiment 1
A kind of biological compound fertilizer, include the material of following parts by weight:45 parts of Trichoderma harzianum tunning, bacillus subtilis 45 parts of bacterium tunning, 400 parts of sargassum.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By bark fiber, mineral matter is with water according to mass ratio 100:3:60 mixing, Access Trichoderma harzianum fermenting agent, access amount be bark fiber, mineral matter and the 0.5% of water gross mass, is fermented 15 days, dry or Dry, clay into power, Trichoderma harzianum tunning is made;The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 5X108cfu/g;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:12:60 mixing, connect Enter fermentation of bacillus subtilis microbial inoculum, access amount is dead leaves, humic acid and the 0.3% of water gross mass, is fermented 20 days, drying or Dry, clay into power, bacillus subtilis (Ehrenberg) Cohn fermented product is made;The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum For 6X108cfu/g;
(3) according to parts by weight, 45 parts of Trichoderma harzianum tunning, 45 parts of bacillus subtilis (Ehrenberg) Cohn fermented product, horse hair are taken 400 parts of algae, is uniformly mixed, is made.
Embodiment 2
A kind of biological compound fertilizer, include the material of following parts by weight:30 parts of Trichoderma harzianum tunning, bacillus subtilis 30 parts of bacterium tunning, 300 parts of yellow tang.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By soybean fiber, mineral matter is with water according to mass ratio 100:1:40 mixing, Access Trichoderma harzianum fermenting agent, access amount be soybean fiber, mineral matter and the 0.6% of water gross mass, is fermented 10 days, dry or Dry, clay into power, Trichoderma harzianum tunning is made;The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 3X108cfu/g;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:10:40 mixing, connect Enter fermentation of bacillus subtilis microbial inoculum, access amount is dead leaves, humic acid and the 0.4% of water gross mass, is fermented 15 days, drying or Dry, clay into power, bacillus subtilis (Ehrenberg) Cohn fermented product is made;The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum For 8X108cfu/g;
(3) according to parts by weight, 30 parts of Trichoderma harzianum tunning is taken, 30 parts of bacillus subtilis (Ehrenberg) Cohn fermented product, steeps leaf 300 parts of algae, is uniformly mixed, is made.
Embodiment 3
A kind of biological compound fertilizer, include the material of following parts by weight:60 parts of Trichoderma harzianum tunning, bacillus subtilis 60 parts of bacterium tunning, 500 parts of green alga.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By abaca fibre, mineral matter is with water according to mass ratio 100:5:80 mixing, Access Trichoderma harzianum fermenting agent, access amount be abaca fibre, mineral matter and the 0.3% of water gross mass, is fermented 20 days, dry or Dry, clay into power, Trichoderma harzianum tunning is made;The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 8X108cfu/g;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:30:80 mixing, connect Enter fermentation of bacillus subtilis microbial inoculum, access amount is dead leaves, humic acid and the 0.4% of water gross mass, is fermented 30 days, drying or Dry, clay into power, bacillus subtilis (Ehrenberg) Cohn fermented product is made;The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum For 8X108cfu/g;
(3) according to parts by weight, 60 parts of Trichoderma harzianum tunning, 60 parts of bacillus subtilis (Ehrenberg) Cohn fermented product, green alga are taken 500 parts, it is uniformly mixed, is made.
Embodiment 4
A kind of biological compound fertilizer, include the material of following parts by weight:45 parts of Trichoderma harzianum tunning, bacillus subtilis 45 parts of bacterium tunning, 25 parts of Ji fern, 400 parts of sargassum.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By bark fiber, mineral matter is with water according to mass ratio 100:3:60 mixing, Access Trichoderma harzianum fermenting agent, access amount be bark fiber, mineral matter and the 0.5% of water gross mass, is fermented 15 days, dry or Dry, clay into power, Trichoderma harzianum tunning is made;The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 5X108cfu/g;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:12:60 mixing, connect Enter fermentation of bacillus subtilis microbial inoculum, access amount is dead leaves, humic acid and the 0.3% of water gross mass, is fermented 20 days, drying or Dry, clay into power, bacillus subtilis (Ehrenberg) Cohn fermented product is made;The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum For 6X108cfu/g;
(3) according to parts by weight, 45 parts of Trichoderma harzianum tunning, 45 parts of bacillus subtilis (Ehrenberg) Cohn fermented product, Ji fern are taken 25 parts, 400 parts of sargassum, it is uniformly mixed, is made.
Embodiment 5
A kind of biological compound fertilizer, include the material of following parts by weight:30 parts of Trichoderma harzianum tunning, bacillus subtilis 30 parts of bacterium tunning, green is the same as 21 parts of leaf moss, 400 parts of sargassum.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By soybean fiber, mineral matter is with water according to mass ratio 100:1:40 mixing, Access Trichoderma harzianum fermenting agent, access amount be soybean fiber, mineral matter and the 0.6% of water gross mass, is fermented 10 days, dry or Dry, clay into power, Trichoderma harzianum tunning is made;The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 3X108cfu/g;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:10:40 mixing, connect Enter fermentation of bacillus subtilis microbial inoculum, access amount is dead leaves, humic acid and the 0.4% of water gross mass, is fermented 15 days, drying or Dry, clay into power, bacillus subtilis (Ehrenberg) Cohn fermented product is made;The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum For 8X108cfu/g;
(3) according to parts by weight, 30 parts of Trichoderma harzianum tunning, 30 parts of bacillus subtilis (Ehrenberg) Cohn fermented product, green are taken With 21 parts of leaf moss, 400 parts of sargassum, it is uniformly mixed, is made.
Comparative example 1
A kind of biological compound fertilizer, include the material of following parts by weight:45 parts of Trichoderma harzianum tunning, sargassum 400 Part.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By bark fiber, mineral matter is with water according to mass ratio 100:3:60 mixing, Access Trichoderma harzianum fermenting agent, access amount be bark fiber, mineral matter and the 0.5% of water gross mass, is fermented 15 days, dry or Dry, clay into power, Trichoderma harzianum tunning is made;The viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 5X108cfu/g;
(2) according to parts by weight, 45 parts of Trichoderma harzianum tunning is taken, 400 parts of sargassum, is made.
Comparative example 2
A kind of biological compound fertilizer, include the material of following parts by weight:30 parts of bacillus subtilis (Ehrenberg) Cohn fermented product, sargassum 400 parts.
The preparation method of above-mentioned biological compound fertilizer, carry out in accordance with the following steps:
(1) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:10:40 mixing, connect Enter fermentation of bacillus subtilis microbial inoculum, access amount is dead leaves, humic acid and the 0.4% of water gross mass, is fermented 15 days, drying or Dry, clay into power, bacillus subtilis (Ehrenberg) Cohn fermented product is made;The viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum For 8X108cfu/g;
(3) according to parts by weight, 30 parts of bacillus subtilis (Ehrenberg) Cohn fermented product is taken, 400 parts of sargassum, is uniformly mixed, It is made.
Experimental example 1
Choose Xingtai area corn trials field, soil moisture content (water:Dry ground) it is 33.40%, pH6.91, soil has Machine matter content 41.9g/kg, NH4+- N 3.1mg/kg, NO3- N 66.8mg/kg, available phosphorus 511.6mg/kg, available potassium 1211.3mg/kg, each experimental group plant 1 mu of corn, apply composite fertilizer prepared by embodiment 1-5 and comparative example 1-2 respectively 50kg, blank control do not apply, and statistics per mu yield is as shown in table 1:
Table 1
Experimental group Per mu yield kg Experimental group Per mu yield kg
Embodiment 1 609 Comparative example 1 543
Embodiment 2 598 Comparative example 2 534
Embodiment 3 602 Blank 532
Embodiment 4 689
Embodiment 5 698
As can be seen from Table 1, embodiment 4-5 yield highest, it is higher than embodiment 1-3 yield, compared with embodiment 1, Other conditions are identical, and only embodiment 4 and embodiment 5 add Ji fern more respectively, and green is the same as leaf moss, it was demonstrated that above-mentioned thing Verify and played a key effect in increase crop yield;Embodiment 1-3 yield is higher than comparative example and blank control, it was demonstrated that combinations thereof Thing has effect for increase crop yield;Embodiment 1-3 adds bacillus subtilis hair less respectively compared with comparative example 1-2 Ferment product, Trichoderma harzianum tunning, it was demonstrated that above-mentioned substance is played a key effect for increase crop yield, and comparative example 1-2 is lacked Few above-mentioned substance is suitable with the effect of blank control, it was demonstrated that bacillus subtilis (Ehrenberg) Cohn fermented product, Trichoderma harzianum tunning can not Individually work, be used in combination just effective.
Experimental example 2
Each experiment weighs the ready soil samples of 600g, and embodiment 1-5 and comparative example 1-2 uniformly mix answering for 30g preparations Hefei, blank do not process.15, the Veronica seed and 15, clearvers seed of each processing difference program request vernalization, water spray Relative humidity 45% is kept, is cultivated 3 days, then measures the bud length of Veronica, clearvers.
The inhibiting rate of weeds is calculated with following formula:
Inhibiting rate %=100 × (blank control weeds bud length-chemicals treatment weeds bud length)/blank control weeds bud length
As a result it is as shown in table 2:
Table 2
Experimental group Inhibiting rate % Experimental group Inhibiting rate %
Embodiment 1 72% Comparative example 1 2%
Embodiment 2 69% Comparative example 2 0%
Embodiment 3 71% Blank ----
Embodiment 4 87%
Embodiment 5 88%
As can be seen from Table 2, embodiment 4-5 inhibiting rate highest, it is higher than embodiment 1-3 inhibiting rates, compare in contrast, it is more Ji fern is added, green is the same as leaf moss, it was demonstrated that above-mentioned substance plays a key effect for suppressing weeds in field;Embodiment 1-3 suppresses Rate is higher than comparative example 1-2, it was demonstrated that above-mentioned composition has effect for suppressing weeds in field;Embodiment 1-3 and comparative example 1-2 phases Than adding bacillus subtilis (Ehrenberg) Cohn fermented product, Trichoderma harzianum tunning less respectively, it was demonstrated that above-mentioned substance is for suppressing field Weeds play a key effect, and comparative example 1-2 lacks above-mentioned substance, suitable with the effect of blank control, it was demonstrated that bacillus subtilis Tunning, Trichoderma harzianum tunning can not individually work, and are used in combination and played a key effect for suppressing weeds in field.
Experimental example 3
Choose the salt-soda soil of Hebei Cangzhou Area, soil moisture content (water:Dry ground) it is 43.40%, pH12.91, Mei Geshi Group 1 mu of corn of plantation to be tested, applies composite fertilizer 50kg prepared by embodiment 1-5 and comparative example 1-2 respectively, blank control does not apply, Count per mu yield and soil pH is as shown in table 3:
Table 3
Experimental group Per mu yield kg PH value
Embodiment 1 389 8.98
Embodiment 2 392 9.32
Embodiment 3 388 9.11
Embodiment 4 451 8.01
Embodiment 5 449 8.02
Comparative example 1 165 11.23
Comparative example 2 158 11.12
Blank 159 12.90
As can be seen from Table 3, embodiment 4-5 per mu yield highest, pH value is minimum, higher than embodiment 1-3 per mu yields, pH value It is low, compare in contrast, add Ji fern, green is with leaf moss more, it was demonstrated that above-mentioned substance reduces soil pH value and risen for corn yield increasing To key effect;Embodiment 1-3 per mu yields are higher than comparative example 1-2, and pH value is low, it was demonstrated that above-mentioned composition is for corn yield increasing, drop Low soil pH value has effect;Embodiment 1-3 adds bacillus subtilis (Ehrenberg) Cohn fermented product less respectively compared with comparative example 1-2, breathes out Thatch trichoderma tunning, it was demonstrated that above-mentioned substance reduces soil pH value and played a key effect, comparative example 1-2 is lacked for corn yield increasing Few above-mentioned substance, it is suitable with the effect of blank control, bacillus subtilis (Ehrenberg) Cohn fermented product, Trichoderma harzianum hair are proved from another point of view Ferment product, for corn yield increasing, reduce soil pH value and play a key effect, and be used in combination just effective.
Disclosed above is only several specific embodiments of the present invention, and still, the present invention is not limited to this, any ability What the technical staff in domain can think change should all fall into protection scope of the present invention.

Claims (9)

1. a kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, it is characterised in that including Trichoderma harzianum tunning, Bacillus subtilis (Ehrenberg) Cohn fermented product, marine alga.
2. marine alga according to claim 1, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, it is characterised in that including such as The material of lower parts by weight:Trichoderma harzianum tunning 30-60 parts, bacillus subtilis (Ehrenberg) Cohn fermented product 30-60 parts, marine alga 300- 500 parts.
3. marine alga according to claim 1, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, it is characterised in that the sea Algae is sargassum, yellow tang, green alga, black algae, sargassum fusifome, the one or more in Sargassum.
4. include the aqua or pulvis of any marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer described in claim 1.
5. the preparation method of the marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer described in claim 1, it is characterised in that Carry out in accordance with the following steps:
(1) prepared by Trichoderma harzianum tunning:By string, mineral matter is with water according to mass ratio 100:(1-5):(40-80) Mixing, Trichoderma harzianum fermenting agent is accessed, ferment 10-20 days, dry, clay into power, Trichoderma harzianum tunning is made;
(2) bacillus subtilis (Ehrenberg) Cohn fermented product:By dead leaves, humic acid is with water according to mass ratio 100:(10-30):(40-80) Mixing, fermentation of bacillus subtilis microbial inoculum is accessed, ferment 15-30 days, dry, clay into power, fermentation of bacillus subtilis is made Product;
(3) according to parts by weight, Trichoderma harzianum tunning 30-60 parts, bacillus subtilis (Ehrenberg) Cohn fermented product 30-60 parts, sea are taken Algae 300-500 parts, are uniformly mixed, are made.
6. the preparation method of marine alga according to claim 4, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, its feature exists It is bark fiber in, the string, soybean fiber, sisal fiber, ramee, oil flax fibre, abaca fibre, hemp is fine One or more in dimension.
7. the preparation method of marine alga according to claim 4, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, its feature exists In the viable bacteria amount contained in the Trichoderma harzianum fermenting agent is 3-8X108cfu/g。
8. the preparation method of marine alga according to claim 4, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer, its feature exists In the viable bacteria amount contained in the fermentation of bacillus subtilis microbial inoculum is 3-8X108cfu/g。
9. marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer described in claim 1 are in crop yield, degeneration-resistant, in pest-resistant Application.
CN201710827568.4A 2017-09-14 2017-09-14 A kind of marine alga, Trichoderma harzianum and bacillus subtilis bacterium composite fertilizer Pending CN107382553A (en)

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CN103708856A (en) * 2014-01-07 2014-04-09 苏州科大微龙信息技术有限公司 Enzyme biological nitrogen fixation fertilizer and preparation method thereof
CN105273719A (en) * 2014-06-24 2016-01-27 王安臻 Preparation process and application of biological soil conditioner
CN106588445A (en) * 2016-11-29 2017-04-26 齐鲁工业大学 Algae microbial soil conditioner specially used for saline-alkali soil and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109232075A (en) * 2018-08-23 2019-01-18 广西乐土生物科技有限公司 Special fertilizer with activity of weeding
CN112266307A (en) * 2020-11-24 2021-01-26 丽江华鸿农业有限公司 Novel organic fertilizer with soil improvement effect
CN112645745A (en) * 2020-12-05 2021-04-13 安丘市天赐生物肥料有限公司 Microbial agent containing trichoderma harzianum and preparation method thereof
CN113373092A (en) * 2021-06-30 2021-09-10 海南金雨丰生物工程有限公司 Compound microbial preparation and preparation method thereof
CN113373092B (en) * 2021-06-30 2023-09-15 海南金雨丰生物工程有限公司 Composite microbial preparation and preparation method thereof
CN115594549A (en) * 2022-11-15 2023-01-13 东营蓝海生态养殖有限公司(Cn) Microbial organic fertilizer for improving saline-alkali soil plough layer structure and preparation method thereof

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Application publication date: 20171124