CN107375879A - A kind of Chinese herbal medicine for preventing sturgeon bacterial disease - Google Patents
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease Download PDFInfo
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- CN107375879A CN107375879A CN201710660321.8A CN201710660321A CN107375879A CN 107375879 A CN107375879 A CN 107375879A CN 201710660321 A CN201710660321 A CN 201710660321A CN 107375879 A CN107375879 A CN 107375879A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
- A61K31/4166—1,3-Diazoles having oxo groups directly attached to the heterocyclic ring, e.g. phenytoin
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- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/51—Gentianaceae (Gentian family)
- A61K36/515—Gentiana
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
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- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/906—Zingiberaceae (Ginger family)
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Abstract
The invention discloses a kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material includes:Chinese gall, honeysuckle, radix glycyrrhizae, ginger, BCDMH.Its raw material also includes:Folium isatidis, the coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, radix scutellariae, felwort, kuh-seng, radix bupleuri, rheum officinale, Herba Andrographitis.The present invention is mutually cooperateed with using Chinese medicine, effectively avoid influenceing sturgeon quality, pollution will not be produced to water body simultaneously, and can be with clearing heat and detoxicating, have antibacterial bacteriostatic concurrently and improve sturgeon immunity, the multi-efficiency for promoting sturgeon to grow, sturgeon various bacterial disease can be played a role simultaneously, have significant prevention effect for fish body compound infection disease caused by a variety of pathogenic originals.
Description
Technical field
The present invention relates to disinfectant technical field, more particularly to a kind of Chinese herbal medicine for preventing sturgeon bacterial disease.
Background technology
In recent years, because the residual problem of medicine causes impact to Export of Chinese Aquatic Products, government is carried out to whole fishing medicine market
Large-scale rectification, discloses large quantities of aquatic products forbidden drugses, such as chloramphenicol, furazolidone, malachite green, pentachlorophenol in succession
Sour sodium etc..In the breeding process of fish, the generation of bacteriosis relatively frequently, occurring area it is also larger.But by its commodity
Value and export market are limited, and cultivation dealer still often carries out the preventing and treating of disease from some out-of-date methods, even with some
Illegal drug, such as malachite green, furazolidone, chloramphenicol control disease.Such prevention and controls, not only make the product of aquatic products
Matter is influenceed by serious, and the breeding wastewater discharged produces direct or indirect pollution to natural environment, can to social
Sustainable development brings serious negative effect.
The content of the invention
Based on technical problem existing for background technology, the present invention proposes a kind of medium-height grass for preventing sturgeon bacterial disease
Medicine, mutually cooperateed with using Chinese medicine, effectively avoid influenceing sturgeon quality, while pollution will not be produced to water body, and can be with
It is clearing heat and detoxicating, have antibacterial bacteriostatic concurrently and improve sturgeon immunity, the multi-efficiency for promoting sturgeon to grow, can be more to sturgeon
Kind bacteriosis plays a role simultaneously, has significant preventing and treating effect for fish body compound infection disease caused by a variety of pathogenic originals
Fruit.
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease proposed by the present invention, its raw material include:Chinese gall, honeysuckle,
Radix glycyrrhizae, ginger, BCDMH.
Chinese gall, sour, mild-natured, return lung warp, kidney channel, large intestine channel.It can astringe the lung, hidroschesis, puckery intestines, controlling nocturnal emission with astringent drugs, stop blooding, removing toxic substances.
Main chronic cough of deficiency lung, spontaneous sweating, protracted dysentery chronic diarrhea, prolapse of the anus, seminal emission, gonorrhoea, various bleedings, carbuncle swells boil.
Honeysuckle, it is sweet, it is cold in nature.Return lung, the heart, stomach.Can clearing heat and detoxicating, wind-heat dissipating.For carbuncle swells furunculosis, larynx numbness,
Erysipelas, warm blood fulminant dysentery, anemopyretic cold, warm disease heating.
Radix glycyrrhizae, mild-natured, sweet, return 12 warps.It can invigorate the spleen and benefit qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, reconcile all
Medicine.For weakness of the spleen and the stomach, lassitude hypodynamia, palpitation, coughing with a lot of sputum, gastral cavity abdomen, four limbs contraction, carbuncle sore tumefacting virus, abirritant
It is thing toxicity, strong.
Ginger, acrid flavour, slightly warm in nature, return lung, spleen, stomach.Energy inducing sweat and dispelling exogenous evils, warming middle energizer to arrest vomiting, warm lung cough-relieving, solving toxin of fish and crab,
Antidote poison.
Preferably, Chinese gall, honeysuckle, radix glycyrrhizae, ginger, the weight ratio of BCDMH are 4-6:1-3:2-4:1-3:4-6.
Preferably, its raw material also includes:Folium isatidis, the coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, radix scutellariae, rough gentian
Grass, kuh-seng, radix bupleuri, rheum officinale, Herba Andrographitis.
Folium isatidis, bitter is cold in nature, returns stomach, the heart channel of Hang-Shaoyin.Can clearing heat and detoxicating, blood cooling and ecchymoses removing.Joined the army for warm heresy, high fever god
Dusk, spot dermexanthesis, jaundice, hot dysentery, mumps, larynx numbness, erysipelas, carbuncle swells.
The coptis, bitter are cold in nature.The thoughts of returning home, spleen, stomach, liver, courage, large intestine channel.Energy heat-clearing and damp-drying drug, purging intense heat and detonicating.For damp and hot ruffian
It is full, acid regurgitation is vomitted, rush down dysentery, jaundice, unconsciousness due to high fever, heart-fire hyperactivity, dysphoria and insomnia, blood-head tells nosebleed, hot eyes, toothache, is quenched one's thirst, carbuncle swells
Furunculosis;Eczema, wet sore are controlled outside, duct is suppurated.
Dandelion, it is sweet, bitter, it is cold in nature, return stomach, Liver Channel.Clearing heat and detoxicating, the dispersing swelling and dissipating binds of energy, inducing diuresis for treating strangurtia.For furunculosis
Pyogenic infections, acute mastitis, scrofula, hot eyes, pharyngalgia, lung carbuncle, acute appendicitis, jaundice with damp-heat pathogen, puckery pain of heat gonorrhea.
Grass-leaved sweetflag, acrid flavour, hardship are warm-natured.The thoughts of returning home, stomach.Energy dampness elimination appetizing, slit phlegm of having one's ideas straightened out, inducing resuscitation intelligence development.For gastral cavity ruffian not
Famine, apnea, coma epilepsy, forgetfulness and deafness.
Ginkgo leaf, it is sweet, bitter, puckery, it is mild-natured.The thoughts of returning home, lung channel.It can astringe the lung, relieving asthma, promoting blood circulation and removing blood stasis, analgesic.For deficiency syndrome of the lung cough
Asthma;Coronary heart disease, angina pectoris, high fat of blood.
Granatum, it is sour, puckery, it is warm-natured, return large intestine channel.Energy relieving diarrhea with astringents, hemostasis, expelling parasite.For chronic diarrhea, protracted dysentery, have blood in stool,
Prolapse of the anus, uterine bleeding, leukorrhea, abdominal pain due to enterositosis.
Radix scutellariae, bitter are cold in nature.Return lung, courage, spleen, large intestine, small intestinl channel.Energy heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis are antiabortive.
For damp-warm syndrome, heat temperature evil of vomitting uncomfortable in chest, damp and hot feeling of fullness, rush down dysentery, jaundice, cough with lung heat, hyperpyrexia polydipsia, blood-head tells nosebleed, carbuncle sore tumefacting virus,
Fetal irritability.
Felwort, bitter are cold in nature.Return liver, gallbladder channel.Can heat-clearing and damp-drying drug;Purging the liver of pathogenic fire is calmed the frightened.For jaundice with damp-heat pathogen;Urine leaching pain;
Swelling of vulva pruritus vulvae;Under humid tropics;The alliteration headache of liver and gall excess fire;Red eye, swell pain;Deaf ear swells;Hypochondriac pain bitter taste;Pyreticosis convulsion.
Kuh-seng, bitter is cold in nature, returns small intestinl channel, Liver Channel, kidney channel, large intestine channel.Can heat-clearing and damp-drying drug;Wind dispelling insecticide.Master is damp and hot to rush down
Dysentery;Hematochezia;Jaundice;Difficult urination;Oedema;Under band;Pruritus vulvae;Mange;Leprosy;Pruitus;Wet poison sore.
Radix bupleuri, bitter, cold nature.Return liver, gallbladder channel.In energy and inducing diaphoresis, soothing the liver, rising Yang.It is past for cold, fever, fever and chills
Come, distending pain in the chest and hypochondrium, irregular menstruation, the prolapse of uterus, prolapse of the anus.
Rheum officinale, bitter is cold in nature, returns stomach, Liver Channel, large intestine channel.Real heat can be rushed down, breaks stagnant, row hemostasis.Cure mainly real heat just
Secret, dyspepsia is stagnated, stomachache, acute appendicitis, acute infectious hepatitis, blood stasis, toothache, bleeding from five sense organs or subcutaneous tissue, acute conjunctivitis;External application
Control burn and scald, suppurative dermatosis, carbuncle swells sore.
Herba Andrographitis, bitter are cold in nature.The thoughts of returning home, lung, large intestine, bladder warp.Clearing heat and detoxicating, the cool blood of energy, detumescence, eliminating dampness.Cure mainly sense
Emit heating, abscess of throat, aphthae, pertussis overstrain cough, dysentery, puckery pain of heat gonorrhea, carbuncle swells sore, venomous snake bite.
Preferably, Chinese gall, folium isatidis, the coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, radix scutellariae, felwort, hardship
Ginseng, radix bupleuri, rheum officinale, the weight ratio of Herba Andrographitis are 4000-6000:20-40:12-24:4-12:4-12:18-27:4-8:2-8:1-
6:2-8:4-12:10-20:6-12.
The application method of the present invention is as follows:Traditional Chinese medicinal components are crushed, add water to cook 2-3h, are filtrated to get filtrate and filter residue,
After wherein full pool spilling head after filtrate cooling, filter residue and BCDMH mixing, it is fitted into gunnysack, being placed in pond makes the property of medicine gradually enter
Pond.
The present invention is mutually cooperateed with using Chinese medicine, effectively avoids influenceing sturgeon quality, and will not cause drug resistance, together
When also will not to water body produce pollution;Using Chinese gall, honeysuckle, radix glycyrrhizae, ginger coordinate be used as major ingredient, can with clearing heat and detoxicating,
Effectively kill the harmful bacteria in sturgeon culturing pond;Again using folium isatidis, the coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, Huang
A kind of reed mentioned in ancient books, felwort, kuh-seng, radix bupleuri, rheum officinale, Herba Andrographitis coordinate major ingredient, have antibacterial bacteriostatic concurrently and improve sturgeon immunity, promote sturgeon
The multi-efficiency to grow, sturgeon various bacterial disease can be played a role simultaneously, for caused by a variety of pathogenic originals
Fish body compound infection disease has significant prevention effect.
Embodiment
Below, technical scheme is described in detail by specific embodiment.
Embodiment 1
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material include:Chinese gall, honeysuckle, radix glycyrrhizae, ginger, bromine
Chlordantoin.
Embodiment 2
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material include by weight:4000 parts of Chinese gall, honeysuckle
3000 parts, 2000 parts of radix glycyrrhizae, 3000 parts of ginger, 4000 parts of BCDMH, 40 parts of folium isatidis, 12 parts of the coptis, 12 parts of dandelion, stone
4 parts of calamus, 27 parts of ginkgo leaf, 4 parts of granatum, 8 parts of radix scutellariae, 1 part of felwort, 8 parts of kuh-seng, 4 parts of radix bupleuri, 20 parts of rheum officinale, punching
6 parts of lotus.
Embodiment 3
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material include by weight:6000 parts of Chinese gall, honeysuckle
1000 parts, 4000 parts of radix glycyrrhizae, 1000 parts of ginger, 6000 parts of BCDMH, 20 parts of folium isatidis, 24 parts of the coptis, 4 parts of dandelion, stone
12 parts of calamus, 18 parts of ginkgo leaf, 8 parts of granatum, 2 parts of radix scutellariae, 6 parts of felwort, 2 parts of kuh-seng, 12 parts of radix bupleuri, 10 parts of rheum officinale, wear
12 parts of heart lotus.
Embodiment 4
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material include by weight:4500 parts of Chinese gall, honeysuckle
2500 parts, 2500 parts of radix glycyrrhizae, 2500 parts of ginger, 4500 parts of BCDMH, 35 parts of folium isatidis, 14 parts of the coptis, 10 parts of dandelion, stone
6 parts of calamus, 24 parts of ginkgo leaf, 5 parts of granatum, 6 parts of radix scutellariae, 2 parts of felwort, 6 parts of kuh-seng, 6 parts of radix bupleuri, 18 parts of rheum officinale, punching
8 parts of lotus.
Embodiment 5
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material include by weight:5500 parts of Chinese gall, honeysuckle
1500 parts, 3500 parts of radix glycyrrhizae, 1500 parts of ginger, 5500 parts of BCDMH, 25 parts of folium isatidis, 22 parts of the coptis, 6 parts of dandelion, stone
10 parts of calamus, 20 parts of ginkgo leaf, 7 parts of granatum, 4 parts of radix scutellariae, 4 parts of felwort, 4 parts of kuh-seng, 10 parts of radix bupleuri, 12 parts of rheum officinale, wear
10 parts of heart lotus.
Embodiment 6
A kind of Chinese herbal medicine for preventing sturgeon bacterial disease, its raw material include by weight:Chinese gall 5kg, honeysuckle
2kg, radix glycyrrhizae 3kg, ginger 2kg, BCDMH 5kg, folium isatidis 30g, coptis 18g, dandelion 8g, grass-leaved sweetflag 8g, ginkgo leaf
22g, granatum 6g, radix scutellariae 5g, felwort 3g, kuh-seng 5g, radix bupleuri 8g, rheum officinale 15g, Herba Andrographitis 9g.
300 tail sturgeons are chosen, are randomly divided into experimental group and control group 1, contrast groups 2, every group of 100 tails;Control group 1 is using normal
Advise prevention method;Control group 2 uses conventional prevention method, and feeds three blooms;And experimental group uses the gained Chinese herbal medicine of embodiment 6
Prevented, and feed three blooms;Remaining cultivating condition is consistent.
The method that the gained Chinese herbal medicine of embodiment 6 is prevented is as follows:By Chinese gall, honeysuckle, radix glycyrrhizae, ginger, folium isatidis,
The coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, radix scutellariae, felwort, kuh-seng, radix bupleuri, rheum officinale, Herba Andrographitis crush, and add decocting
2-3h is boiled, filtrate and filter residue, wherein full pool spilling head after filtrate cooling is filtrated to get, after filter residue and BCDMH mix, loads fiber crops
In bag, being placed in pond makes the property of medicine gradually enter pond, while stirs water by aerator.
The concrete operations for feeding three blooms are as follows:It is 5 in mass ratio by rheum officinale, golden cypress, radix scutellariae:3:2 are crushed into powder mixing
Uniformly obtain three blooms;Per the 100kg sturgeons blooms of 4kg tri-, three blooms are uniformly spread on pellet, treat to dry completely,
Feed, feed daily 1 time, continuous 3-5 days after well mixed.
After raising 60 days, its result is as follows:
Control group 1 | Control group 2 | Experimental group | |
Body weight growth rate, % | 53.6 | 87.9 | 104.3 |
Illness rate, % | 15 | 8 | 1 |
The death rate, % | 3 | 2 | 0 |
Bacteriosis illness rate, % | 11 | 4 | 0 |
The bacteriosis death rate, % | 2 | 1 | 0 |
As seen from the above table:Gained Chinese herbal medicine of the invention has antibacterial bacteriostatic and improves sturgeon immunity, promotes sturgeon growth
The multi-efficiency of development.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (4)
1. a kind of Chinese herbal medicine for preventing sturgeon bacterial disease, it is characterised in that its raw material includes:It is Chinese gall, honeysuckle, sweet
Grass, ginger, BCDMH.
2. prevent the Chinese herbal medicine of sturgeon bacterial disease according to claim 1, it is characterised in that Chinese gall, honeysuckle, sweet
Grass, ginger, the weight ratio of BCDMH are 4-6:1-3:2-4:1-3:4-6.
3. the Chinese herbal medicine of prevention sturgeon bacterial disease according to claim 1 or claim 2, it is characterised in that its raw material also includes:
Folium isatidis, the coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, radix scutellariae, felwort, kuh-seng, radix bupleuri, rheum officinale, Herba Andrographitis.
4. according to the Chinese herbal medicine of any one of the claim 1-3 prevention sturgeon bacterial diseases, it is characterised in that Chinese gall,
Folium isatidis, the coptis, dandelion, grass-leaved sweetflag, ginkgo leaf, granatum, radix scutellariae, felwort, kuh-seng, radix bupleuri, rheum officinale, the weight of Herba Andrographitis
It is 4000-6000 to measure ratio:20-40:12-24:4-12:4-12:18-27:4-8:2-8:1-6:2-8:4-12:10-20:6-12.
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