CN107375700B

CN107375700B - A herbal compound preparation for preventing and treating male infertility

Info

Publication number: CN107375700B
Application number: CN201710487678.0A
Authority: CN
Inventors: 宋元达; 赛德侯赛因; 杨俊换
Original assignee: Shandong University of Technology
Current assignee: Shandong University of Technology
Priority date: 2017-06-23
Filing date: 2017-06-23
Publication date: 2020-09-29
Anticipated expiration: 2037-06-23
Also published as: CN107375700A

Abstract

The invention provides a herbal medicine compound preparation for preventing and treating male infertility, which is prepared by extracting and concentrating roots of sansevieria trifasciata, seeds of Anthriscus angularis, seeds of Mucuna pruriens, seeds of Mimosa pudica, gum arabic, radix astragali, seed coats of Plantago asiatica, kapok gum and Eurycoma longifolia with ethanol, methanol, acetone and water.

Description

A herbal compound preparation for preventing and treating male infertility

Technical Field

The invention belongs to the field of plant extraction, and particularly relates to a herbal medicine compound preparation for preventing and treating male infertility.

Background

In human infertility, male infertility amounts to 50%, whereas in male infertile patients the cause of the disease is unknown to 40-50% of the total, with approximately 8% of the patients seeking herbal treatment. There are many causes of male infertility, and different causes require different treatment modalities. Male infertility can be reduced in various biochemical indicators, especially in the amount of sperm, in the physiopathological examination.

In order to improve male sexual function, various treatment methods are used, for example, psychologists or psychologists, who give patients confidence in psychotherapy to obtain treatment; can also be solved by surgical equipment or surgical implantation of the penis, etc.; androgen hormone therapy, particularly testosterone, or other specific drugs can be used to maintain erection, desire levels, and orgasm in men. Other therapies to supplement or correct the diagnosis and treatment of male infertility are also under constant investigation, e.g. human chorionic gonadotropin (hCG), hormonal therapy, In Vitro Fertilization (IVF), etc.

At present, in the process of treating male infertility, a plurality of disadvantages cannot be avoided, such as high treatment cost, complex condition in surgical operation and risk of operation failure, and headache, dizziness, blurred vision, blushing, nasal obstruction, penis persistent erection and the like can be caused by drug treatment. Despite the advances in modern therapeutic approaches, effective, inexpensive drugs are still in need of development. For centuries, people in countries around the world have used local plants to seek solutions to male infertility. Thus, plant-derived herbs have been selected to complement the treatment of male infertility, and their effects in improving male sexual desire and performance have also been demonstrated.

Now, Indian herbs are accepted by people because of their clinical effects and low manufacturing cost. Many Indian herbs have the effect of stimulating sexual function, and the root of Indian cymbidium, seed of Antrodia angustifolia, seed of Mucuna pruriens, seed of Mimosa pudica, acacia gum, radix astragali, seed coat of Plantago asiatica, kapok gum, and Eurycoma longifolia root have obvious effect. In animal experiments, research has shown that the traditional Chinese medicine composition has the effect of stimulating sexual function, but the traditional Chinese medicine composition is not carried out in human body experiments, so that human body experiments are needed to provide theoretical basis for the herbal medicine preparation to treat male sexual function, and the traditional Chinese medicine composition has important significance for researching natural product medicines with low price and high quality to replace synthetic medicines with high price and many side effects.

Disclosure of Invention

In view of the defects of the existing research, the invention aims to provide a compound herbal preparation for preventing and treating male sexual dysfunction and application thereof by carrying out human body tests on a compound herbal preparation of roots of sansevieria trifasciata, seeds of Anthriscus androsaceus, seeds of Mucuna pruriens, seeds of Mimosa pudica, gum arabic, roots of Astragalus, seed coats of Plantago asiatica, kadsura japonica and roots of Eurycoma longifolia.

The technical scheme of the implementation of the invention is as follows: a compound preparation of herbal medicines for preventing and treating male infertility is prepared from methanol, ethanol, acetone, and water extract of the herbal medicines, and can be used for preparing medicines for treating male infertility.

The herbal compound extract is prepared by the following method: collecting the roots of the triangle orchid, the seeds of the Anthriscus manihot, the seeds of the mucuna pruriens, the seeds of the mimosa pudica, gum arabic, the roots of astragalus membranaceus, the seed coats of semen plantaginis, kapok gum and the roots of Eurycoma longifolia, cleaning, removing impurities, drying in the sun, powdering, grinding 15-30g of dry powder under the protection of liquid nitrogen, sequentially putting the ground dry powder into a 250ml triangular flask filled with 30-100ml of ethanol, methanol, acetone and water, extracting for 16-20 hours under the conditions of stirring speed of 280rpm, extraction temperature of 15-50 ℃ and darkness, filtering the extracting solution, collecting filtrate, evaporating and concentrating the filtrate at 40 ℃, and freeze-drying, and storing at-80 ℃ for later.

The medicine for treating male infertility comprises the extract and pharmaceutically acceptable carriers or conventional non-staple foods, such as capsules, tablets, oral liquid, functional diet and the like.

Advantageous effects

Compared with the prior art, the invention has the following beneficial technical effects:

the herbal medicine compound preparation has obvious effect on treating male infertility, can improve the concentration, the activity, the power and the amount of sperms in the semen of the male and can increase the content of male hormones in the blood, so the herbal medicine compound preparation has potential utilization value on developing the treatment medicines for treating male sexual dysfunction and aspermia.

Detailed Description

The following detailed description of the embodiments provided by the present invention is given with reference to the examples:

selecting 38 male infertility patients with age of 22-40 years and sperm number of 5-20 × 10 in semen⁶Each ml, the sperm motility is in the range of 10% -25%, the sperm moving forward is less than 15%, and the sperm morphology is normalThe rate is less than 70%. All patients had a healthy spouse.

Randomly dividing 38 male patients into two groups, wherein one group is a control group, and the number of the male patients is 15; one group was experimental, and the number of people was 23. The control group patients took capsules containing 250mg of placebo, while the experimental group took capsules containing 250mg of the compound herbal extract three times daily for 90 days.

The formula of the compound herbal preparation comprises: the root of the trigonostema, the seed of the Anthriscus manihot, the seed of the mucuna pruriens, the seed of the mimosa pudica, the gum arabic, the root of astragalus membranaceus, the seed coat of plantain seed, the collodion and the root of eurycoma longifolia have a remarkable effect in a proportion of 10-30% in the compounding process, and the proportion of the extract in the embodiment is 1:1:1:1:1:1:8, and the extract is compounded to prepare a capsule containing 250mg of effective components for use.

Example 1:

the physical quality index of 38 male patients is measured, and the average value of each index is calculated, and the result is shown in table 1:

table 1: index of physical quality of male patient

Example 2:

and respectively collecting the semen of two groups of male patients after administration for 0, 60 and 90 days, and performing semen routine index project examination. The results of the amount of sperm are shown in Table 2:

table 2: post-dose examination of the amount of sperm in a male patient

As shown in Table 2, in the semen routine examination, the semen volume is more than or equal to 2ml, the semen is milky white and semi-fluid, the semen is liquefied to be normal within 30min, the semen volume of the male patients in the experimental group is obviously increased after taking the herbal medicine compound preparation compared with the control group, the semen volume is recovered to be normal after taking the herbal medicine compound preparation for 60 days, and the effect of increasing the semen volume along with the prolonging of the taking time is obvious.

Example 3:

and respectively collecting the semen of two groups of male patients after administration for 0, 60 and 90 days, and performing semen routine index project examination. The results of sperm concentration are shown in Table 3:

table 3: post-dose sperm concentration examination in male patients

As can be seen from Table 3, the normal sperm density was > 20 × 10 in routine examination of semen⁶The sperm concentration of the male patients taking the herbal compound preparation per ml is recovered to be normal after taking the herbal compound preparation for 60 days compared with the placebo group, and the sperm concentration is increased more along with the prolonging of the taking time.

Example 4:

and respectively collecting the semen of two groups of male patients after administration for 0, 60 and 90 days, and performing semen routine index project examination. Sperm motility results are shown in table 4:

table 4: sperm motility examination of post-dose male patients

From 4, in the semen routine examination, the normal sperm motility a grade is more than 25%, and the sperm motility of the male patients taking the herbal compound preparation compared with the placebo group is recovered to be normal after taking the herbal compound preparation for 60 days, and is increased more along with the prolonging of the taking time.

Example 5:

blood of two groups of male patients was collected after administration for 0 and 90 days, respectively, and plasma testosterone examination was performed. The plasma testosterone results are shown in table 5:

table 5: post-dose plasma testosterone examination of male patients

As can be seen from Table 5, in the examination of testosterone in plasma, the testosterone level in plasma was increased in the male patients who took the herbal compound preparation compared with the placebo group after taking 90 days.

Example 6:

blood was collected from two groups of male patients at 0 and 90 days after administration, respectively, and plasma hormone examination was performed. The results of the plasma hormone are shown in table 6:

table 6: post-dose plasma hormone testing of male patients

As can be seen from Table 6, in the examination of the hormone levels in males, the hormone levels in the plasma were increased after 90 days of administration in male patients who took the herbal combination compared to the placebo group.

From examples 1 to 6, it is known that the herbal compound preparation can effectively improve the health level of male infertility patients, increase the amount of semen, density and motility level of sperm, and significantly increase the levels of male testosterone and hormone, so the herbal compound preparation has potential value for being developed into drugs or related foods for treating male infertility.

Claims

1. A herbal compound preparation for preventing and treating male infertility is characterized in that: the preparation method of the herbal medicine compound preparation comprises the following steps: collecting roots of cymbidium tricornutum, seeds of Anthrisci acutus, seeds of Mucuna pruriens, seeds of Mimosa pudica, gum arabic, roots of Astragalus membranaceus, seed coats of Plantain seeds, kapok gum and roots of Eurycoma longifolia, cleaning, removing impurities, drying in the sun, powdering, grinding 15-30g of dry powder under the protection of liquid nitrogen, sequentially putting the ground powder into a 250ml triangular bottle filled with 30-100ml of ethanol, methanol, acetone and water, extracting at the stirring speed of 280rpm and the extraction temperature of 15-50 ℃ in the dark for 16-20 hours, filtering the extracting solution, collecting filtrate, evaporating and concentrating at the temperature of 40 ℃, freeze-drying, storing at the temperature of-80 ℃ for later use, and compounding according to the ratio of 1:1:1:1:1:8 to prepare the compound.

2. The herbal compound preparation for preventing and treating male infertility according to claim 1, further comprising a pharmaceutically acceptable carrier.