CN107373450A - A kind of ultra micro high calcium deep-sea collagen powder and its preparation method and application - Google Patents
A kind of ultra micro high calcium deep-sea collagen powder and its preparation method and application Download PDFInfo
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- CN107373450A CN107373450A CN201710610685.5A CN201710610685A CN107373450A CN 107373450 A CN107373450 A CN 107373450A CN 201710610685 A CN201710610685 A CN 201710610685A CN 107373450 A CN107373450 A CN 107373450A
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- collagen powder
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- 201000005671 spondyloarthropathy Diseases 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009747 swallowing Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013337 tricalcium citrate Nutrition 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention relates to a kind of preparation method of ultra micro high calcium deep-sea collagen powder, step：Under the collective effect of alkali protease and konjaku flour, fish-bone is subjected to tumbling, protectiveness enzymolysis, obtains the fish-bone after tumbling enzymolysis；Fish-bone after obtained tumbling enzymolysis is mixed 23 times with water, and autoclave sterilization, the fish-bone after being sterilized are used after draining；Fish-bone after obtained sterilizing is dried to water content less than 10%；80 150 mesh are crushed to pulverizer again, are once crushed fish-bone；Obtained once crushing fish-bone is subjected to ultralow temperature nanometer pulverization to nanoscale, obtains ultra micro high calcium deep-sea collagen powder.Using optimization protectiveness purification, the beneficiating ingredient loss such as calcium, phosphorus, bone collagen peptide, chondroitin, DHA and EPA, sclerotin vitamin A, D is reduced, reaches the purpose imitated and replenished the calcium entirely.And nano bone protocalcium of the present invention possesses high absorptivity, can reach 80 90%.
Description
Technical field
The present invention relates to a kind of ultra micro high calcium deep-sea collagen powder, further relates to a kind of preparation side of ultra micro high calcium deep-sea collagen powder
Method and application.
Background technology
Calcium is one of indispensable nutrient of human body, if without calcium, lived can not be produced.Calcium is
Most abundant mineral matter in human body, the whole life process of human body is participated in, be human life sheet.From bone formation, contraction of muscle,
Heartbeat, the thinking activities of nerve and brain, until the growing of human body, dispelling fatigue, brain tonic and intelligence development and delaying to decline
Wait for a long time, it may be said that all motions of life all be unable to do without calcium.Daily intake that calcium amount is enough, the new old of human normal could be maintained
Metabolism, adaptive faculty of the enhancing human body to living environment.Calcium can strengthen the endurance of people, make one energetic, mental toughness.Internal calcium
Abundance, it could effectively prevent cerebral hemorrhage, cancer and cardiopathic generation, be advantageous to have good health and a long life.
Substantial amounts of calcium in bone and tooth, it is the main component for forming body tissue, and bone is had certain hardness, rises
The effect of support body.Calcium in blood, have and maintain brain and cardiac function normal, bear all normal cell physiology situations
Regulation and the effect such as secreting hormone, solidificating blood, cell there is no calcium just can not survive.Effect of the calcium in human body is as follows：
1) existence and the function of cell are maintained.Cell division is bred, and number is cumulative, changes function gradually with unicellular, all
Need the participation of calcium.Calcium wakes up cell and started working, otherwise cell is always maintained at sleep state from extracellular entrance.Calcium enters
Cell, electric wave is sent, and be covered with the nerve fibre of whole body, form human body information network, the input of information passes through the activity of calcium
Pass to parts of body.EndocrineAlso blood, the transmission information into organ must be passed through by calcium during gland cell secreting hormone.Cell
Individual feature and the network that intercommunicates can not all lack calcium, or even aging, disease, the dead equilibrium theory that can use calcium
It is bright.Fully take in calcareous, cell can just keep fit active.
2) participation is nervimuscular stress process.On a cellular level, as the lotus root between N＆M Excitation-contraction
Join the factor, medical education net, which is collected, to be arranged | promote the conditioning agent of neurotransmitter release and endo-exocrine gland secreting hormone, conduction god
Through impulsion, heart beat rhythm is maintained.Calcium has sedation, and when calcium concentration reduces in body fluid, the excitability of N＆M increases,
There is Spontaneous Contraction in muscle, is had a convulsion when serious, when calcium concentration increase in body fluid, then suppresses the excitement of N＆M
Property.
3) calcium maintains to maintaining internal acid-base balance and the internal many biochemical processes of regulation is required, it can promote body
The activity of interior a variety of enzymes, it is a variety of zymoexciters, such as lipase, amylase are by regulation of calcium.When internal calcium deficiency
When protein, fat, carbohydrate can not make full use of, cause malnutrition, apocleisis, constipation, hypoevolutism, immunologic function
Decline.
4) calcium is a kind of clotting factor, plays catalytic action when prothombin is fibrin ferment, then fibrin ferment makes
Fibrinogen, which is polymerized to fibrin, makes blood clotting.Calcium is combined with phosphatide, maintains the integrality and permeability of cell membrane.Calcium
Ion can make body fluid normal through cell membrane, commonly used to alleviate changing for the membrane permeability pressure caused by the diseases such as allergy
Become.
Calcium deficiency can cause more than 100 kinds of disease, such as：
1st, bone metabolic disease：Osteoproliferation, osteoporosis, cervical spondylopathy, scapulohumeral periarthritis, rickets, osteomalacia, caput femoris necrosis
Deng；
2nd, endocrine disease：Hyperthyroidism, diabetes, adrenal gland, obesity etc.；
3rd, it is cardiovascular：Atherosclerosis, hypertension, heart disease, miocardial infarction etc., pregnancy-hypertension syndrome etc.；
4th, nervous system：Neurasthenia, hyperactivity, dementia, the cerebrovascular, cerebral hemorrhage, epileptics etc.；
5th, digestive system：Peptic ulcer, hepatic sclerosis, gastrointestinal convulsion, hemorrhoid etc.；
6th, respiratory system：Bronchial spasm, pneumonia, pulmonary tuberculosis, chronic obstructive pulmonary disease etc.；
7th, urinary system：Oedema, acute nephritis, acute or chronic renal failure, stone in urinary system etc.；
8th, reproductive system：Infertility, dysmenorrhoea, climacteric syndrome etc.；
9th, sense organ：Myopia, cataract, brothers are split, Hearing, skin disease, eczema, Zu Xian, Shou Xian etc.；
10th, immune system：Dermatitis, rheumatism, rheumatoid, arthritis, allergy skin disease, CTD etc.；
11st, hematological system：Anaemia, hemophilia etc..
Calcic is about 1200 grams in adult body in the case of normal, wherein about 99% is present in bone and tooth, mainly
Exist in the form of hydroxyapatite crystal, maintain bone and tooth that there is hard structure and support, in addition about 1% calcium Chang Yiyou
From or the ionic condition of combination be present in outside soft tissue cells and in liquid and blood, play the work of important regulation of physiological functions
With being referred to as miscible calcium pool.Miscible calcium pool maintains dynamic equilibrium with the calcium in bone, i.e., the calcium in bone is constantly from osteoclastic thin
Discharged in born of the same parents into miscible calcium pool, ensure that the concentration of plasma calcium remains constant；And the calcium in miscible calcium pool is constantly deposited on
Gegenbaur's cell.The renewal of this calcium, adult are about daily 700 milligrams, and the renewal speed of calcium slows down with advancing age,
The bone of child updates once per 1-2, and the bone about 10-12 of adult could update once.After male about 18 years old, bone
Length start stabilization, more earlier, the rate of development of height tapers off women, but the density of sclerotin continues to increased numbers
Year.Generally after 35 years old, the content of the inorganic substances such as calcium in bone gradually decreases, such as insufficient supplement calcium material, can
Phenomena such as serious disease including osteoporosis can occur caused by calcium deficiency, women overweights male.For all one's life of people
For replenish the calcium and can not all lack.
The composition of bone tissue can be divided into 3 kinds of organic species, inorganic salts material and moisture.Organic species are by collagen egg
In vain, glycoprotein, polysaccharide, bone matrix, collagenous fibres etc. are formed, including cell component and cytoplasm.Cell component includes skeletonization
Cell, osteoclast and osteocyte；Cytoplasm is made up of bone matrix and collagenous fibres.Organic matter makes bone have necessarily tough
Property.The inorganic matter of bone is also known as bone salts, and main component is calcium and phosphorus.Bone salts accounts for the 75% of compact bone substance total amount, mainly with hydroxyl
The form of base apatite crystal is present.Bone salts is combined with collagenous fibres and matrix makes bone have certain degree of hardness.Calcium in inorganic matrix
There is provided bone hardness and pressure, the collagenous fibres in organic substrate provide support and tension force.
Modern medicine study finds that absorption of the human body to calcium is a complicated process, but has any certainly,
Human body is easiest to absorb to the calcium source of natural animal, particularly the natural animal calcium higher with skeleton composition similarity
Source is to be easiest to absorb, and an outstanding feature of these natural calciums is exactly that main component is colloidal calcium phosphate, colloidal calcium phosphate
Main component be calcium phosphate, its most significant feature is：The calcium of product：Phosphorus=1.7 (mol ratio), the ratio and human body bone
The calcium-phosphorus ratio of bone and tooth is completely the same.Colloidal calcium phosphate and common inorganic calcium phosphate are not fully equivalent, and it is a kind of height
The macromolecule calcium phosphate salt/polymer of interlaced polymeric, spy are referred to as Ultra-polyphosphate (UP) or Hydroxy-Apatite
(HAP)。
Present people increasingly pay attention to replenishing the calcium and replenish the calcium the research of raw material and preparation, and the form of in the market calcium agent is various each
Sample, but mainly have calcium carbonate, calcium citrate, calcium lactate, calcium gluconate etc.；Also occur in recent years several with natural animal collagen
Calcium-supplementing preparation prepared by raw material, they are made into the appearance of the forms such as tablet, capsule, effervescent tablet, oral liquid, chewable tablets, by
To the favor in market.
Modern medicine study shows, human body is about 20% to the absorptivity of inorganic calcium, to the absorptivity of common organic calcium about
For 30%, therefore, research is new, safe, reasonable, efficient absorption to replenish the calcium raw material and preparation increasingly by scientific worker's
Pay attention to.People have invested research object emphasis the fish higher with skeleton similarity, a collection of using fish-bone as natural calcium source
The technical literature for preparing replenish the calcium raw material and calcium-supplementing preparation is disclosed in succession, and the security and calcium for greatly improving calcium-supplementing preparation are inhaled
Yield.
Most common fish-bone hydrolysis and calcium extracting method have three kinds：Enzymatic isolation method, acid system and alcohol alkaline process.Three kinds of methods are each favourable
Disadvantage：Acid system and alcohol alkaline process cost are low, and method is simple, but the destruction to nutritional ingredient is bigger；Enzymatic isolation method reaction condition ratio
It is relatively gentle, but extraction time length, and enzyme is expensive, makes processing cost too high.In existing literature report, with to three of the above
The linguistic term and process optimization of method are in the majority.But also there are document report microbial fermentation, high-pressure pulse electric processing etc.
Other extracting methods.Its public technology is mainly summarized as follows method：
1st, enzymatic isolation method
Enzymatic isolation method is a kind of method that solubility calcium composition is discharged with protease hydrolytic fish bone collagen albumen substrate, such as with flounder
Fish-bone is raw material, prepares bone collagen polypeptide chelating calcium using enzymatic isolation method, discovery uses volume ratio as 3:1 polypeptide liquid is (with alkalescence
Protease digests 1h under the conditions of ph=8, temperature 60 C and is made) with bone meal acid hydrolysis solution be chelatropic reaction liquid, ph=8,40 DEG C
Under the conditions of the obtained product calcium chelant rate highests of reaction 40min, reach 98.64%.
2nd, acid system
Being usually used in hydrolyzing fish-bone and the acid of calcium extraction mainly has hydrochloric acid, lactic acid, acetic acid etc., in addition, with citric acid and apple
It is also one of current study hotspot that the mixed acid of acid extracts CMC calcium activateds from fish-bone.Such as to different acid concentrations, different temperatures bar
The effect of hydrochloric acid, lactic acid and acetic acid extraction fish-bone calcium is studied under part, it is found that the extraction effect of hydrochloric acid is best, be can reach
More than 60%, the recovery rate of lactic acid is only 30% or so, and the recovery rate of acetic acid is minimum.
3rd, alcohol alkaline process
Cod bone calcium powder is such as produced using NaOH and acetic acid immersion, it is found that optimal procedure parameters soak for 1mol/LNaOH solution
30h, 60% acetic acid immersion 15h are steeped, and has produced under the technological parameter calcic 27.8%, phosphorous 12.2%, fat content
Close to 0 cod bone calcium powder.Compared with Acid hydrolysis, fish-bone calcium powder prepared by alcohol alkaline process has more preferable color and luster and less different
Taste, and fat content substantially reduces, and serves the effect of degreasing to a certain extent, is more beneficial for absorption of the human body to calcium.
4th, other method
Such as influence of the high electric field pulse electric field to citric acid and malic acid extraction fish-bone calcium, research finds to join in optimum process
Dissolution rate reaches 83.86% under said conditions, and one times is higher by than traditional acid hydrolysis method under equal conditions.If ball-milling treatment is due to drop
The low granular size of fishbone dust, the recovery rate of silver carp bone meal calcium is substantially increased, and as the extension of Ball-milling Time, calcium carry
Take the trend that rate is in first increases and then decreases.
Study above-mentioned fish-bone and prepare calcium source public technology of replenishing the calcium and be not difficult to find out, either enzymatic isolation method, acid system, alcohol alkaline process or its
Other party method prepares fish-bone calcium, is all to improve the dissolution rate of calcium, recovery rate, calcium chelant rate as target, ignore full nutrition completely
The comprehensive importance replenished the calcium.Meanwhile prior art and final products are primarily present following open defect：
1. fail comprehensive effectively utilize in fish-bone containing calcium replenishing and the necessary bone collagen of health, chondroitin, widow
Poly- peptide (oligomerization polypeptide phosphate), and the various trace elements such as phosphorus, magnesium, iron, zinc and vitamin；
2. fish-bone resource fails to be fully used；
3. complex manufacturing, high expensive；
4. the calcium powder of acid, alkali, alcohol alkaline process production, it is actually that natural organic calcium source is become inorganic calcium；
5. the products such as the raw material calcium produced and calcium tablet, coarse mouthfeel, it is difficult to swallow, application receives substantially
Limitation；
6. the sorption enhanced rate of calcium fails to reach higher level.
Therefore, it is necessary to propose effective technical scheme, solve the above problems.
The content of the invention
The present invention is directed to above-mentioned the deficiencies in the prior art, there is provided a kind of preparation method of ultra micro high calcium deep-sea collagen powder.Should
Method simple production process, in good taste, the sorption enhanced rate of calcium is high.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of preparation method of ultra micro high calcium deep-sea collagen powder, this method comprise the following steps：
(1) under the addition of alkali protease and konjaku flour, fish-bone is subjected to tumbling enzymolysis, obtains the fish after tumbling enzymolysis
Bone；
The alkaline protease activity is 200000-300000U/g, and the basic protein enzyme dosage is fish-bone quality
0.1-0.2%；
The konjaku flour dosage is the 0.2-0.5% of fish-bone quality；
The tumbling enzymatic hydrolysis condition is：PH value 10-11,45-55 DEG C of temperature, hour time 1-1.5, roll in constant temperature tumbler
Rub enzymolysis；
(2) fish-bone after the tumbling obtained in step (1) is digested is mixed 2-3 times with water, and high temperature is used after draining
Autoclaving, the fish-bone after being sterilized；
The weight ratio of fish-bone and water after the tumbling enzymolysis is 1:(3-5)；
(3) fish-bone after the sterilizing that will be obtained in step (2) is dried to water content less than 10%；It is crushed to again with pulverizer
80-150 mesh, is once crushed fish-bone；
(4) the once crushing fish-bone obtained in step (3) is subjected to ultralow temperature nanometer pulverization to nanoscale, obtains ultra micro height
Calcium deep-sea collagen powder.
Preferably, the process conditions of ultralow temperature nanometer pulverization are in the step (4)：In ultralow temperature nano grinder, 0
Crushed to -40 DEG C of ultralow temperature, 4000-8000 beats/min of vibrations crushed work frequency, amplitude 0.5-0.8cm.
Preferably, use in the step (2) process conditions of HTHP quick sterilization for：Autoclave sterilization kettle
In, 121 DEG C, 2.5mpa.s pressure sterilization 5-10 minutes.
Preferably, the process conditions of drying are in the step (3)：In dryer, 55-65 DEG C is dried to water content and is less than
10%.
Preferably, fish-bone is deep-sea fish bone in the step (1).
Preferably, pH value 10-11 is to adjust pH value to 10-11 using sodium acid carbonate in the step (1).
Beneficial effect：
It is raw material to make full use of with the pollution-free fish-bone in deep-sea of skeleton composition similarity more than 99.9%, using optimization
Protectiveness purifies, and reduces the beneficiating ingredient damage such as calcium, phosphorus, bone collagen peptide, chondroitin, DHA and EPA, sclerotin vitamin A, D
Lose, reach the purpose imitated and replenished the calcium entirely；
Using hyperfrequency, ultralow temperature nanometer pulverization technology, adequately protect be not denatured fully effectively while, nanometer
The absorption of calcium can be obviously promoted by crushing；Modern medicine study surface, it is to be not easy with (calcium routine calcium) existing for compound form
It is absorbed, to reach easy absorption, calcium first must be ionized easily, and ultralow temperature nano bone protocalcium has superpower ionization ratio
Rate, conventional calcium just start to be ionized in hydrochloric acid in gastric juice, and nano bone protocalcium will be higher by much than the ionization fraction of other calcium, this hair
It is bright that there is extra high absorptivity, it can reach 80-90%.
Fish-bone resource can be fully used, hence it is evident that improve resource utilization, reduce pollutant emission, be beneficial to ring
Protect in border；Simultaneously, hence it is evident that improve calcium source value and relative worth cost；
Calcium source eating mouth feel is significantly improved, has reached population and has changed, smooth moist mouthfeel；It is suitable for as medicine of replenishing the calcium
Product, calcium supplement health care product, calcium-enriching function food calcium source use；Conventional calcium tablet coarse mouthfeel can substantially be eliminated, it is difficult to the drawbacks of swallowing.
The present invention also provides a kind of ultra micro high calcium deep-sea collagen powder.The collagen powder is prepared using above-mentioned preparation method,
The collagen powder footpath is 20-900nm.
The full trophic structure of collagen powder.It has been sufficiently reserved in fish-bone to health beneficiating ingredient, has not only increased and replenish the calcium
Effect, other functions of the calcium source to health are also substantially extended, particularly：
(1) nutriment necessary to DHA, EPA brain, has been effectively retained, man memory power and the power to think can be improved, reached
Child's intelligence development, adult prevent intelelectual deterioration, old man from preventing dull-witted purpose.
(2), rational calcium-phosphorus ratio is provided, while bone collagen peptide, chondroitin, sclerotin vitamin are provided, makes the suction of calcium
Receive, convert, utilization rate significantly improves, prevents a loss ability from substantially strengthening；Reach reasonable high efficiency calcium-enriching, while reduce calcium loss
Purpose.
(3) oligomeric peptide (oligomerization polypeptide phosphate), being effectively kept in fish-bone, there is anti-oxidant, removing free radical, carry
High immunity, the function of preventing aging, meanwhile, oligomerization polypeptide phosphate has extremely strong adsorption capacity to calcium, is more conducive to calcium
Absorb.
(4) chondroitin, has been effectively kept, there is antitumor, anti-gene mutation, improve human immunological competence, suppress white
Blood disease cell proliferation, improve osteoporosis, prevent and treat the effect of rheumatic arthritis.
The present invention also provides a kind of application of ultra micro high calcium deep-sea collagen powder in medicine field.
The present invention also provides a kind of application of ultra micro high calcium deep-sea collagen powder in field of health care products.
The present invention also provides a kind of application of ultra micro high calcium deep-sea collagen powder in field of food.
Embodiment
The present invention provides a kind of efficient, multi-functional, natural, safe, high-quality calcium source of replenishing the calcium.A kind of ultra micro is specially provided
The preparation method of high calcium deep-sea collagen powder, its technological process are：De- meat deep-sea fish bone-clean-drain-tumbling-protectiveness
Enzymolysis-rinse-drain-autoclave sterilization-low temperature drying-Ordinary pulverization-ultralow temperature nanometer pulverization-packaging-is super
Micro- high calcium deep-sea collagen powder.
In above-mentioned technological process, wherein tumbling, protectiveness enzymolysis the step for the appropriate biological enzymolysis of the present invention, optimization are protected
It is important for the purification of shield property.The tumbling, protectiveness enzymolysis step are specially：Fish-bone is put into constant temperature tumbler, added
(alkaline protease activity is 200000-300000U/g to alkali protease, and the basic protein enzyme dosage is fish-bone quality
0.1-0.2%) and 0.2-0.5% konjaku flours, adjust pH value 10-11.0 with sodium acid carbonate and (can also be that other alkalescence are adjusted
Agent, such as sodium hydroxide, sodium phosphate, calcium hydroxide, sodium tripolyphosphate, for adjusting pH value), 45-55 DEG C of constant temperature is warming up to, is rolled
Rub enzymolysis 1-1.5 hours, reach fish-bone surface bone and flesh be completely separated, the purpose that the flesh of fish dissolves.
Wherein ultralow temperature nanometer pulverization the step for be important for purposes of the invention.Using ultralow temperature, high frequency nano powder
Broken technology, while avoiding processing from being denatured, the collagen calcium powder of nanosizing greatly improves the rate of ionization of calcium, human body to calcium
Absorptivity, conversion ratio, utilization rate, reach the purpose of high-quality calcium source.The food mouthfeel of calcium source is lifted simultaneously and is expanded applies
Scope.Ultralow temperature nanometer pulverization step is specially：Fishbone dust will be crushed to add in the broken machine of ultralow temperature nanometer, 0 to -40 DEG C of ultralow temperature
It is crushed to nanoscale, 4000-8000 beats/min of vibrations crushed work frequency, amplitude 0.5-0.8cm.
The present invention use autoclave sterilization, sterilized while, fully reduce collagen composition and is denatured.
The present invention uses low temperature drying technology, hence it is evident that reduces collagen composition denaturation rate.
Embodiment 1
A kind of preparation method of ultra micro high calcium deep-sea collagen powder, this method comprise the following steps：
The pollution-free fish-bone in deep-sea is cleaned with clear water, drained away the water；It is put into constant temperature tumbler, adds alkali protease
(300000U/g about 0.1-0.2% add a small amount of water dissolving) and 0.2-0.5% konjaku flours, pH value 10- is adjusted with sodium acid carbonate
11.0, it is warming up to 45-55 DEG C of constant temperature, tumbling enzymolysis 1-1.5 hours, reaches that fish-bone surface bone and flesh is completely separated, the flesh of fish dissolves
Purpose.The fish-bone that will have been digested, by bone：Water=1:4 ratio mixes 2-3 times, drains, wherein, filtered fluid can be with first
For reclaiming protein peptides；Fish-bone after draining is put into autoclave sterilization kettle, 121 DEG C, 2.5mpa.s pressure sterilizations 5-10
Minute；Fish-bone after sterilizing is put into dryer, 55-65 DEG C is dried to water content less than 10%；Drying fish-bone is used commonly
Pulverizer is crushed to 100 mesh or so.Fishbone dust will be crushed to add in the broken machine of ultralow temperature nanometer, vibrations crushed work frequency 4000-
8000 beats/min, amplitude 0.5-0.8cm, 0 to -40 DEG C of ultralow temperature is crushed to nanoscale and can packed.
The selection of fish-bone species of the present invention is various, is suitable for the production of various fish-bone calcium sources, preferably from free of contamination depth
Ocean fish bone has ensured security, natural sex, the collagen similitude of calcium source as raw material.
The present invention reduces ossein egg inside fish-bone using the high viscosity tack and reduction permeability of konjaku amidin
White enzymolysis denaturation.
Preparation method of the present invention is due to thoroughly improving the structure of calcium source, mouthfeel and binding characteristic with conventional food materials, energy
Calcium source prepared by the obvious calcium source application present invention for expanding calcium-enriching function food, conventional medicine of replenishing the calcium is lifted except that can be used as
The quality, efficiently outer of product, health products, the also a variety of calcium-enriching function food of suitable for producing, such as high-calcium fish intestines high calcium sausages, height
A variety of high-calcium foods such as calcium meat products, high calcium Flour product, high dairy, high calcium vegetable protein beverage, high calcium solid beverage.
The present invention can be summarized with others without prejudice to the concrete form of the spirit or essential characteristics of the present invention.Therefore, nothing
By from the point of view of which point, the embodiment above of the invention can only all be considered the description of the invention and can not limit this hair
Bright, claims indicate the scope of the present invention, and above-mentioned explanation does not point out the scope of the present invention, therefore, with this
Any change in claims of invention suitable implication and scope, all it is considered as the claim for being included in the present invention
In the range of book.
Claims (10)
1. a kind of preparation method of ultra micro high calcium deep-sea collagen powder, it is characterised in that this method comprises the following steps：
(1) under the addition of alkali protease and konjaku flour, fish-bone is subjected to tumbling enzymolysis, obtains the fish-bone after tumbling enzymolysis；
The alkaline protease activity is 200000-300000U/g, and the basic protein enzyme dosage is the 0.1- of fish-bone quality
0.2%；
The konjaku flour dosage is the 0.2-0.5% of fish-bone quality；
The tumbling enzymatic hydrolysis condition is：PH value 10-11,45-55 DEG C of temperature, hour time 1-1.5, tumbling enzyme in constant temperature tumbler
Solution；
(2) fish-bone after the tumbling obtained in step (1) is digested is mixed 2-3 times with water, and HTHP is used after draining
Sterilizing, the fish-bone after being sterilized；
The weight ratio of fish-bone and water after the tumbling enzymolysis is 1:(3-5)；
(3) fish-bone after the sterilizing that will be obtained in step (2) is dried to water content less than 10%；Again 80- is crushed to pulverizer
150 mesh, are once crushed fish-bone；
(4) the once crushing fish-bone obtained in step (3) is subjected to ultralow temperature nanometer pulverization to nanoscale, obtains ultra micro high calcium depth
Extra large collagen powder.
2. the preparation method of ultra micro high calcium deep-sea collagen powder as claimed in claim 1, it is characterised in that in the step (4)
The process conditions of ultralow temperature nanometer pulverization are：In ultralow temperature nano grinder, 0 to -40 DEG C of ultralow temperature crushes, and shakes crushed work
4000-8000 beats/min of frequency, amplitude 0.5-0.8cm.
3. the preparation method of ultra micro high calcium deep-sea collagen powder as claimed in claim 2, it is characterised in that in the step (2)
Use the process conditions of HTHP quick sterilization for：In autoclave sterilization kettle, 121 DEG C, 2.5mpa.s pressure sterilizations 5-10
Minute.
4. the preparation method of ultra micro high calcium deep-sea collagen powder as claimed in claim 3, it is characterised in that in the step (3)
The process conditions of drying are：In dryer, 55-65 DEG C is dried to water content less than 10%.
5. the preparation method of ultra micro high calcium deep-sea collagen powder as claimed in claim 1, it is characterised in that in the step (1)
Fish-bone is deep-sea fish bone.
6. the preparation method of ultra micro high calcium deep-sea collagen powder as claimed in claim 1, it is characterised in that in the step (1)
PH value 10-11 is to adjust pH value to 10-11 using sodium acid carbonate.
7. a kind of ultra micro high calcium deep-sea collagen powder, it is characterised in that using the ultra micro high calcium deep-sea bone as described in claim 1-6
The preparation method of former powder is prepared, and the collagen powder footpath is 20-900nm.
A kind of 8. application of ultra micro high calcium deep-sea collagen powder in medicine field.
A kind of 9. application of ultra micro high calcium deep-sea collagen powder in field of health care products.
A kind of 10. application of ultra micro high calcium deep-sea collagen powder in field of food.
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| CN113303472A (en) * | 2021-06-08 | 2021-08-27 | 广西壮族自治区农业科学院 | Functional beverage produced by using litsea cubeba and preparation process thereof |
| CN113317506A (en) * | 2021-06-08 | 2021-08-31 | 广西壮族自治区农业科学院 | Solid beverage for reducing uric acid and preparation method thereof |
| CN114874290A (en) * | 2022-06-27 | 2022-08-09 | 华中农业大学 | Silver carp steak antioxidant peptide, separation method and silver carp steak antioxidant peptide chewable tablet |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110101033A (en) * | 2019-05-30 | 2019-08-09 | 泉州师范学院 | A kind of preparation method of the instant fishbone dust of nanometer |
| CN113303472A (en) * | 2021-06-08 | 2021-08-27 | 广西壮族自治区农业科学院 | Functional beverage produced by using litsea cubeba and preparation process thereof |
| CN113317506A (en) * | 2021-06-08 | 2021-08-31 | 广西壮族自治区农业科学院 | Solid beverage for reducing uric acid and preparation method thereof |
| CN114874290A (en) * | 2022-06-27 | 2022-08-09 | 华中农业大学 | Silver carp steak antioxidant peptide, separation method and silver carp steak antioxidant peptide chewable tablet |
| CN114874290B (en) * | 2022-06-27 | 2023-08-15 | 华中农业大学 | Chub raft antioxidant peptide and separation method thereof, and chub raft antioxidant peptide chewable tablet |
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