CN110101033A - A kind of preparation method of the instant fishbone dust of nanometer - Google Patents
A kind of preparation method of the instant fishbone dust of nanometer Download PDFInfo
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- CN110101033A CN110101033A CN201910464017.5A CN201910464017A CN110101033A CN 110101033 A CN110101033 A CN 110101033A CN 201910464017 A CN201910464017 A CN 201910464017A CN 110101033 A CN110101033 A CN 110101033A
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- fish
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- fishbone dust
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- calcium
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- 239000000428 dust Substances 0.000 title claims abstract description 73
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 20
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 18
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 18
- 241000251468 Actinopterygii Species 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 16
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- 239000000839 emulsion Substances 0.000 claims abstract description 14
- 238000000265 homogenisation Methods 0.000 claims abstract description 14
- 238000012545 processing Methods 0.000 claims abstract description 11
- 230000008569 process Effects 0.000 claims abstract description 9
- 238000013329 compounding Methods 0.000 claims abstract description 8
- 230000003993 interaction Effects 0.000 claims abstract description 8
- 239000011148 porous material Substances 0.000 claims abstract description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 23
- 230000001376 precipitating effect Effects 0.000 claims description 19
- 244000199866 Lactobacillus casei Species 0.000 claims description 12
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 12
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 12
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 12
- 229940017800 lactobacillus casei Drugs 0.000 claims description 12
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 12
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 12
- 238000000498 ball milling Methods 0.000 claims description 8
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 238000005238 degreasing Methods 0.000 claims description 6
- 239000002781 deodorant agent Substances 0.000 claims description 6
- 239000004519 grease Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 238000009826 distribution Methods 0.000 claims description 5
- 239000006166 lysate Substances 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 241000186605 Lactobacillus paracasei Species 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 239000011575 calcium Substances 0.000 abstract description 30
- 229910052791 calcium Inorganic materials 0.000 abstract description 30
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 29
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 8
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 8
- 238000004090 dissolution Methods 0.000 abstract description 5
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 235000021190 leftovers Nutrition 0.000 abstract 1
- 229960005069 calcium Drugs 0.000 description 27
- 241000894006 Bacteria Species 0.000 description 10
- 239000006071 cream Substances 0.000 description 10
- 210000000988 bone and bone Anatomy 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 235000013351 cheese Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000000227 grinding Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 229960003563 calcium carbonate Drugs 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000001354 calcination Methods 0.000 description 1
- 150000001669 calcium Chemical class 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 239000004227 calcium gluconate Substances 0.000 description 1
- 229960004494 calcium gluconate Drugs 0.000 description 1
- 235000013927 calcium gluconate Nutrition 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001970 hydrokinetic effect Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- -1 salt calcium Class Chemical class 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
- A23L5/17—General methods of cooking foods, e.g. by roasting or frying in a gaseous atmosphere with forced air or gas circulation, in vacuum or under pressure
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/70—Comminuted, e.g. emulsified, fish products; Processed products therefrom such as pastes, reformed or compressed products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Abstract
The invention discloses a kind of preparation methods of the instant fishbone dust of nanometer, belong to technical field of aquatic product processing.It chooses Ba Lang fish fish-bone and crushes and cross 200 meshes, fish-bone mud is prepared after dissolution, then add compounding zymophyte fermentation enzymatic hydrolysis 0.5 ~ 2 h of fish-bone mud, to improve the content of water-soluble calcium in fish-bone；Stable emulsion is further formed by 5 ~ 10 min of homogenization of dynamic high-pressure microfluidizer, the interaction pipette tips that pore size is 75 μm are loaded onto when second homogenate processing, it is prepared into nanoscale fish-bone powder, reaches best instant effect using 75 ~ 85 DEG C of warm water.The nanoscale fishbone dust that the present invention combines secondary dynamic high-pressure microjet preparation by compounding bacterial strain fermenting enzyme solution can be applied in infusing potable, effectively increase the amount of dissolution of calcium ion, and improve the dissolubility of fishbone dust, exploitation for aquatic product leftovers higher value application and high calcium content infusing potable provides the foundation, simple process, operability, applicability are wide.
Description
Technical field
The invention belongs to technical field of aquatic product processing, are related to a kind of preparation method of instant fishbone dust of nanometer.
Background technique
Fishbone dust refers to the leftover bits and pieces such as remaining fish-bone, fish-skin, internal organ after aquatic fish processing, by drying, crushing etc.
Manufactured leftover bits and pieces powder after process processing, mainly based on fish-bone.Fish-bone is mainly by the axis bone of fish body, appendage bone and fishbone group
At, the 30 ~ 40% of Zhan Xianyu total weight, containing the metal ions such as a large amount of protein, moisture, fat and Ca, Fe, Zn abundant,
It is a kind of nutritive value processing byproduct abundant.China pair is still within starting with the processing technology of fishbone dust and application at present
In the stage, a part of fishbone dust is used as animal feed and sells at a low price, most of then be simply discarded, and both wastes vast resources,
It polluted environment again.
After fish-bone is broken into fishbone dust, nutritional ingredient is easier to be absorbed by organisms utilization.Current domestic and international existing research
It is middle to use acid, alkali process hydrolysis fishbone dust, but this method needs the reaction time longer, destroys to nutritional ingredient more serious；Yang Xianqing
Equal researchers use enzymatic hydrolysis fishbone dust to improve solubility calcium content, including pepsin, lipase, nuclease etc.；This
Outside, for microbial fermentation enzymatic hydrolysis also by Preliminary Applications in the enzymatic hydrolysis preparation of fishbone dust, main common bacteria is lactic acid bacteria.Mechanical lapping
It is the widely used technology of current factory, including ultramicro grinding (refers to using mechanical or hydrokinetic method, carries out to material
The operation such as mill, impact, shearing, to overcome solid interior cohesiveness that it is made to be broken into molecule), dry ball milling, high energy
The technologies such as wet ball grinding, high temperature and pressure crushing, high speed crushing, air-flow crushing and anoxybiotic calcining.Its partial size after fish-bone grinding degradation
It is the key factor for determining its characteristic, reduces dissolubility, retentiveness and bioavailability that partial size is remarkably improved fishbone dust.Mesh
Nanoscale fishbone dust is prepared using ball-milling method in preceding existing research, but handled using dynamic high-pressure microfluidizer there is not yet
Specific report.
Calcium is the mineral matter element that content is most in human body, and metabolic balance is vital to maintaining human health to play
Effect.Mainly there are the two major classes calcium-supplementing preparations such as inorganic and organic calcium salt, biological bone calcium class at present, wherein inorganic, organic salt calcium
Class includes calcium carbonate, calcium gluconate, calcium lactate etc., and this kind of calcium preparation has the disadvantages of water-soluble not high, absorptivity is lower, the
In two class calcium supplementing products, having natural biological bone calcium includes fish-bone calcium, Yak Bone calcium and chelating calcium preparation etc., studies have shown that biological
In bone calcium, miniaturization fishbone dust calcium absorptivity with higher and calcium Retention have the calcium carbonate for being significantly higher than corresponding dosage.
Therefore the content for improving solubility calcium in fishbone dust has important scientific meaning to the nutrition and application value that improve fishbone dust.Horse
Hai Xia et al. is research shows that carry out the content that fermentation is remarkably improved free calcium ions to Tilapia mossambica fishbone dust using lactic acid bacteria.Poplar
Refined dawn et al. uses acidity extraction solubility calcium, and the recovery rate of solubility calcium is up to 28.45% under optimal conditions.Domestic and international researcher
Fermentation is carried out to livestock and poultry bone using microorganism and prepares nutrient solution, so that reference state calcium therein is converted into free calcium, improves people
Body, in the deposition of human body, but improves using microorganism compounding fermentation fish-bone the rarely seen report of research of free calcium to the absorption of calcium and calcium
Road.Meanwhile in fishbone dust, the content of water-soluble calcium is influenced by fishbone dust particle size.
Dynamic high-pressure microjet homogeneous technology integrates the multi unit operations such as conveying, mixing, wet pulverizing, homogeneous
Physical modification technology.Its principle is to utilize high speed shear, high-frequency vibration, convection current shock, instantaneous pressure decline, cavitation, short
When ultra high pressure treatment and the combination that continuously runs so that material reached preferable particle refinement, be evenly distributed, realize nanometer
The preparation of grain.Compared with traditional high-pressure homogeneous method, microjet homogeneous can make the structural and functional significant change of particle,
It is effectively improved the Interfacial Adsorption characteristic and stability of lotion.This method is in the preparation of fishbone dust there is not yet specific report.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to a kind of preparation method of instant fishbone dust of nanometer is provided,
The content for effectively increasing water-soluble calcium ion in fishbone dust reduces the partial size of fishbone dust, so as to improve the molten of fishbone dust
Solution property and retentiveness, and improve the nutritive value utilization rate of fishbone dust.
For achieving the above object, technical program of the present invention lies in:
A kind of preparation method of the instant fishbone dust of nanometer, the specific steps are as follows:
1. Ba Lang fish fish-bone leftover bits and pieces is sufficiently cleaned, according to fish-bone and sodium carbonate liquor solid-liquid ratio 1g:2 ~ 5mL, fish-bone is soaked
30 ~ 50min in the sodium carbonate liquor of 40wt.% is steeped, fishy smell and grease are sloughed；
It is dried 2. the Ba Lang fish fish-bone of deodorant degreasing is cleaned again, preliminary dry pulverization process is carried out using ball milling instrument, crosses 200 mesh
Sieve is added 3 ~ 4 times of fishbone dust quality of water and is diluted, stirs evenly, obtain fish-bone mud；
3. adjusting fish-bone mud temperature is 30 ~ 40 DEG C, zymophyte (cheese is compounded according to the addition of the ratio of fishbone dust dry weight 3:250
Lactobacillus (Lactobacillus casei) and plant cream barBacterium(Lactobacillus plantarum) freeze-dried powder according to
Mass ratio 2:0.5 ~ 1 is compounded) biofermentation 0.5 ~ 2 h of enzymatic hydrolysis is carried out, fermentation system pH value is 7.0 ~ 7.5；
4. after enzymatic hydrolysis, using dynamic high-pressure microfluidizer to carry out a homogenization high for 90 MPa, 40 DEG C of dynamic
A 5 ~ 10min of homogeneous in microfluidizer is pressed, stable emulsion is formed；Take stable emulsion, dynamic high-pressure microfluidizer dress
Upper pore size is 75 μm of interaction pipette tips, and 90MPa, 10 ~ 15min of homogeneous again under the conditions of 40 DEG C are prepared into component point
Cloth is uniform, the fish-bone powder liquid that partial size is nanometer, system is uniform；
5. the fish-bone powder liquid is carried out centrifugal treating using centrifuge, nanoscale fishbone dust precipitating is obtained, and by precipitated powder
End is washed 2 ~ 3 times using clear water, and centrifugal treating, collects precipitating fishbone dust again；
6. precipitating fishbone dust is placed in vacuum freeze dryer, it is dried and obtains nanoscale fish-bone powder；
7. the nanoscale fish-bone powder is dissolved using 75 ~ 85 DEG C of warm water, the fishbone dust that dissolubility is good, system is uniform can be obtained
Lysate.
Beneficial benefit of the invention is:
1, in fish-bone powder, preparation method thereof of the invention, using Lactobacillus casei (Lactobacillus casei) and plant cream barBacterium(Lactobacillus plantarum) fermentation hydrolysis fish-bone mud, in biological fermentation process, lactic acid bacteria producing enzyme are compounded in proportion
Hydrolysis, so that the combination calcium in fish-bone is efficiently converted into free calcium, so that the content of water-soluble calcium is significantly improved, most
The content of calcium ion, which is compared, under excellent parameter does not have processing group to improve nearly 4 times；
2, it in fish-bone powder, preparation method thereof of the invention, is handled using dynamic high-pressure microjet, liquid system uniform particle diameter, stabilization,
And partial size reaches Nano grade, not only further improves the content of free calcium, while more effectively making the hydrophobic of fish-bone powder particles
Group and polar group exposure, the exposure of these groups is so that the combination of itself and hydrone enhances, to improve fishbone dust
The dissolubility and retention ability of grain, wherein nanometer fishbone dust dissolubility in 75 DEG C of warm water is best, for further exploitation fishbone dust speed
Molten drink has established technical foundation.
Specific embodiment
Further to disclose rather than the present invention is limited, the present invention is described in further detail below in conjunction with example.
Embodiment 1
1. Ba Lang fish fish-bone leftover bits and pieces is sufficiently cleaned, according to fish-bone and sodium carbonate liquor solid-liquid ratio 1g:3mL, fish-bone is impregnated
The 40min in 40% sodium carbonate liquor, sloughs fishy smell and grease；
It is dried 2. the Ba Lang fish fish-bone of deodorant degreasing is cleaned again, preliminary dry pulverization process is carried out using ball milling instrument, crosses 200 mesh
Sieve is added 3 times of fishbone dust quality of water and is diluted, stirs evenly, obtain fish-bone mud；
3. adjusting fish-bone mud temperature is 35 DEG C, zymophyte (cheese cream bar is compounded according to the addition of the ratio of fishbone dust dry weight 3:250
Bacterium (Lactobacillus casei) and plant cream barBacterium(Lactobacillus plantarum) freeze-dried powder according to quality
Compounded than 2:1) biofermentation 1.5 h of enzymatic hydrolysis are carried out, fermentation system pH value is 7.0；
4. after enzymatic hydrolysis, using dynamic high-pressure microfluidizer to carry out a homogenization as 90 MPa, 40 DEG C of dynamic is high
5 min of homogeneous in microfluidizer is pressed, stable emulsion is formed, takes stable emulsion, dynamic high-pressure microfluidizer loads onto hole
Diameter size is 75 μm of interaction pipette tips, 90MPa, and it is equal to be prepared into component distribution by 10 min of homogeneous again under the conditions of 40 DEG C
Fish-bone powder liquid even, that partial size is nanometer, system is uniform；
5. the fish-bone powder liquid is carried out centrifugal treating using centrifuge, nanoscale fishbone dust precipitating is obtained, and by precipitated powder
End is washed 3 times using clear water, and centrifugal treating, collects precipitating fishbone dust again；
6. precipitating fishbone dust is placed in vacuum freeze dryer, it is dried and obtains nanoscale fish-bone powder；
7. the nanoscale fish-bone powder is dissolved using 75 DEG C of warm water, it is molten that the fishbone dust that dissolubility is good, system is uniform can be obtained
Solve liquid.
Embodiment 2
1. Ba Lang fish fish-bone leftover bits and pieces is sufficiently cleaned, according to fish-bone and sodium carbonate liquor solid-liquid ratio 1g:2mL, fish-bone is impregnated
The 30min in the sodium carbonate liquor of 40wt.%, sloughs fishy smell and grease；
It is dried 2. the Ba Lang fish fish-bone of deodorant degreasing is cleaned again, preliminary dry pulverization process is carried out using ball milling instrument, crosses 200 mesh
Sieve is added 3 times of fishbone dust quality of water and is diluted, stirs evenly, obtain fish-bone mud；
3. adjusting fish-bone mud temperature is 40 DEG C, zymophyte (cheese cream bar is compounded according to the addition of the ratio of fishbone dust dry weight 3:250
Bacterium (Lactobacillus casei) and plant cream barBacterium(Lactobacillus plantarum) freeze-dried powder according to quality
Compounded than 2:0.5) biofermentation 0.5 h of enzymatic hydrolysis is carried out, fermentation system pH value is 7.0；
4. after enzymatic hydrolysis, using dynamic high-pressure microfluidizer to carry out a homogenization as 90 MPa, 40 DEG C of dynamic is high
10 min of homogeneous in microfluidizer is pressed, stable emulsion is formed, takes stable emulsion, dynamic high-pressure microfluidizer loads onto hole
Diameter size is 75 μm of interaction pipette tips, 90MPa, and it is equal to be prepared into component distribution by 15 min of homogeneous again under the conditions of 40 DEG C
Fish-bone powder liquid even, that partial size is nanometer, system is uniform；
5. the fish-bone powder liquid is carried out centrifugal treating using centrifuge, nanoscale fishbone dust precipitating is obtained, and by precipitated powder
End is washed 2 times using clear water, and centrifugal treating, collects precipitating fishbone dust again；
6. precipitating fishbone dust is placed in vacuum freeze dryer, it is dried and obtains nanoscale fish-bone powder；
7. the nanoscale fish-bone powder is dissolved using 80 DEG C of warm water, it is molten that the fishbone dust that dissolubility is good, system is uniform can be obtained
Solve liquid.
Embodiment 3
1. Ba Lang fish fish-bone leftover bits and pieces is sufficiently cleaned, according to fish-bone and sodium carbonate liquor solid-liquid ratio 1g:3mL, fish-bone is impregnated
The 40min in the sodium carbonate liquor of 40wt.%, sloughs fishy smell and grease；
It is dried 2. the Ba Lang fish fish-bone of deodorant degreasing is cleaned again, preliminary dry pulverization process is carried out using ball milling instrument, crosses 200 mesh
Sieve is added 3 times of fishbone dust quality of water and is diluted, stirs evenly, obtain fish-bone mud；
3. adjusting fish-bone mud temperature is 35 DEG C, zymophyte (cheese cream bar is compounded according to the addition of the ratio of fishbone dust dry weight 3:250
Bacterium (Lactobacillus casei) and plant cream barBacterium(Lactobacillus plantarum) freeze-dried powder according to quality
Compounded than 2:1) biofermentation 1.5 h of enzymatic hydrolysis are carried out, fermentation system pH value is 7.0；
4. after enzymatic hydrolysis, using dynamic high-pressure microfluidizer to carry out a homogenization as 90 MPa, 40 DEG C of dynamic is high
5 min of homogeneous in microfluidizer is pressed, stable emulsion is formed, takes stable emulsion, dynamic high-pressure microfluidizer loads onto hole
Diameter size is 75 μm of interaction pipette tips, 90MPa, and it is equal to be prepared into component distribution by 10 min of homogeneous again under the conditions of 40 DEG C
Fish-bone powder liquid even, that partial size is nanometer, system is uniform；
5. the fish-bone powder liquid is carried out centrifugal treating using centrifuge, nanoscale fishbone dust precipitating is obtained, and by precipitated powder
End is washed 3 times using clear water, and centrifugal treating, collects precipitating fishbone dust again；
6. precipitating fishbone dust is placed in vacuum freeze dryer, it is dried and obtains nanoscale fish-bone powder；
7. the nanoscale fish-bone powder is dissolved using 100 DEG C of warm water, it is molten that the fishbone dust that dissolubility is good, system is uniform can be obtained
Solve liquid.
Embodiment 4
1. Ba Lang fish fish-bone leftover bits and pieces is sufficiently cleaned, according to fish-bone and sodium carbonate liquor solid-liquid ratio 1g:5mL, fish-bone is impregnated
In the sodium carbonate 40min of 40 wt%, fishy smell and grease are sloughed；
It is dried 2. the Ba Lang fish fish-bone of deodorant degreasing is cleaned again, preliminary dry pulverization process is carried out using ball milling instrument, crosses 200 mesh
Sieve is added 3 times of fishbone dust quality of water and is diluted, stirs evenly, obtain fish-bone mud；
3. adjusting fish-bone mud temperature is 35 DEG C, zymophyte (cheese cream bar is compounded according to the addition of the ratio of fishbone dust dry weight 3:250
Bacterium (Lactobacillus casei) and plant cream barBacterium(Lactobacillus plantarum) freeze-dried powder according to quality
Compounded than 2:0.5) biofermentation 2 h of enzymatic hydrolysis are carried out, fermentation system pH value is 7.0；
4. after enzymatic hydrolysis, using dynamic high-pressure microfluidizer to carry out a homogenization as 90 MPa, 40 DEG C of dynamic is high
5 min of homogeneous in microfluidizer is pressed, stable emulsion is formed, takes stable emulsion, dynamic high-pressure microfluidizer loads onto hole
Diameter size is 75 μm of interaction pipette tips, 90MPa, and it is equal to be prepared into component distribution by 10 min of homogeneous again under the conditions of 40 DEG C
Fish-bone powder liquid even, that partial size is nanometer, system is uniform；
5. the fish-bone powder liquid is carried out centrifugal treating using centrifuge, nanoscale fishbone dust precipitating is obtained, and by precipitated powder
End is washed 2 times using clear water, and centrifugal treating, collects precipitating fishbone dust again；
6. precipitating fishbone dust is placed in vacuum freeze dryer, it is dried and obtains nanoscale fish-bone powder；
7. the nanoscale fish-bone powder is dissolved using 75 DEG C of warm water, it is molten that the fishbone dust that dissolubility is good, system is uniform can be obtained
Solve liquid.
Detection method
1. the measurement of calcium ion content
1 g fishbone dust is taken, is dissolved in 5 mL warm water, 50 mL volumetric flask of constant volume measures calcium using atomic absorption spectrophotometer
Content.
Table explanation
The content of calcium ion in each case study on implementation of table 1
Note: Lactobacillus casei and lactobacillus plantarum group are independent strain fermentation enzymatic hydrolysis, and additive amount is 3:250(bacterial strain freeze-dried powder: fish
Bone meal)
A: blank group, no enzymatic hydrolysis and homogenization
B: compounding enzymolysis processing, no homogenization
C: without enzymolysis processing, direct homogenization
D: compound enzyme solution joint homogenization
The result shows that according to parameter Lactobacillus casei (Lactobacillus casei) and lactobacillus plantarum
(Lactobacillus plantarum) freeze-dried powder according to mass ratio 2:1, after 1.5 h of fermentation enzymatic hydrolysis, using 90 MPa, 40
DEG C 5 min of homogeneous of dynamic high-pressure microfluidizer, forms stable emulsion, takes stable emulsion, dynamic high-pressure microfluidizer
Loading onto pore size is 75 μm of interaction pipette tips, 90MPa, under the conditions of 40 DEG C again after 10 min of homogeneous, with 75 DEG C of temperature
Water dissolution, solubility calcium content highest improve nearly 4 times compared to blank group A.And use Lactobacillus casei or lactobacillus plantarum
The content of soluble calcium can be improved in independent fermentation process, but compared with Compound bacterium group, effect is poor, illustrates Compound bacterium
Calcium ion the amount of dissolution can preferably be improved.Simultaneously, the results showed that, the compound proportion of bacterial strain compares the influence of the amount of dissolution of calcium ion
Homogenization is bigger, and nanometer fishbone dust has best solubility at 75 DEG C, too high or too low for temperature all to influence fishbone dust
Dissolubility and stability of solution there is significant aggregate and precipitate phenomenon especially under the conditions of 100 DEG C.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (6)
1. a kind of preparation method of the instant fishbone dust of nanometer, which comprises the following steps:
(1) Ba Lang fish fish-bone leftover bits and pieces is sufficiently cleaned, according to fish-bone and sodium carbonate liquor solid-liquid ratio 1 g:2 ~ 5 mL, by fish-bone
It is soaked in 30 ~ 50min in the sodium carbonate liquor of 40wt.%, sloughs fishy smell and grease；
(2) the Ba Lang fish fish-bone of deodorant degreasing is cleaned again and is dried, preliminary dry pulverization process is carried out using ball milling instrument, crosses 200
Mesh is added 3 ~ 4 times of fishbone dust quality of water and is diluted, stirs evenly, obtain fish-bone mud；
(3) adjusting fish-bone mud temperature is 30 ~ 50 DEG C, and compounding zymophyte is added and carries out biofermentation enzymatic hydrolysis；
(4) after digesting, twice homogenization processing is carried out using dynamic high-pressure microfluidizer, it is uniform to obtain nanoscale, system
Fish-bone powder liquid；
(5) the fish-bone powder liquid is subjected to centrifugal treating, obtains nanoscale fishbone dust precipitating, and precipitating powder is used into clear water
Washing 2 ~ 3 times, centrifugal treating, collects precipitating fishbone dust again；
(6) precipitating fishbone dust is placed in vacuum freeze dryer, is dried and obtains nanoscale fish-bone powder；
(7) the nanoscale fish-bone powder is dissolved using warm water, obtains the fishbone dust lysate that dissolubility is good, system is uniform.
2. the method according to claim 1, wherein the compounding zymophyte additive amount according to fishbone dust dry weight
Ratio 3:250 addition, compound zymophyte in Lactobacillus paracasei (Lactobacillus casei) and lactobacillus plantarum
(Lactobacillus plantarum) freeze-dried powder carry out compounding addition according to mass ratio 2:0.5 ~ 1.
3. the method according to claim 1, wherein the compounding zymophyte is fermented, enzymolysis time is 0.5 ~ 2 h,
Fermentation system pH value is 7.0 ~ 7.5.
4. the method according to claim 1, wherein described use the first time homogenization of dynamic high-pressure microjet
For 5 ~ 10min of homogeneous in 90 MPa, 40 DEG C of dynamic high-pressure microfluidizer, stable emulsion is formed.
5. the method according to claim 1, wherein described use second of the homogenization of dynamic high-pressure microjet
For the lotion that first time homogeneous obtains, dynamic high-pressure microfluidizer loads onto the interaction pipette tips that pore size is 75 μm,
90MPa, 10 ~ 15min of homogeneous again under the conditions of 40 DEG C, is prepared into uniform component distribution, partial size is that the fishbone dust of Nano grade is micro-
Grain.
6. the method according to claim 1, wherein the fishbone dust lysate uses solvent for 75 ~ 85 DEG C
Warm water.
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| CN110604268A (en) * | 2019-08-28 | 2019-12-24 | 浙江海洋大学 | Preparation method and application of cuttlefish ink nanoparticles |
| WO2022061973A1 (en) * | 2020-09-22 | 2022-03-31 | 尚朝阳 | Canned fish and preparation method therefor |
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| CN107485030A (en) * | 2017-07-31 | 2017-12-19 | 浦江县昂宝生物技术有限公司 | A kind of low value fish-bone polypeptide chelate calcium |
| CN109527441A (en) * | 2018-11-15 | 2019-03-29 | 韶关市华工高新技术产业研究院 | It is a kind of to be starched using the method and manufactured fish-bone of super-voltage micro jet technology production high active calcium fish-bone slurry |
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| CN106616528A (en) * | 2016-11-30 | 2017-05-10 | 山东好当家海洋发展股份有限公司 | Preparation method for fermentation type fish bone powder chewable tablets |
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