CN107372644A - A kind of agricultural chemicals for being used to prevent and treat nematode - Google Patents
A kind of agricultural chemicals for being used to prevent and treat nematode Download PDFInfo
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- CN107372644A CN107372644A CN201710418606.0A CN201710418606A CN107372644A CN 107372644 A CN107372644 A CN 107372644A CN 201710418606 A CN201710418606 A CN 201710418606A CN 107372644 A CN107372644 A CN 107372644A
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- nematode
- essential oil
- avermectin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of agricultural chemicals for being used to prevent and treat nematode, its active component is made up of Avermectin B2 and cinnamon essential oil, and the mass ratio of the Avermectin B2 and cinnamon essential oil is 15: 15.Because cinnamon essential oil has repellent, kills the double action of nematode, after the two compounding, one side cinnamon essential oil can significantly reduce invasion of the nematode to crop root, harm；On the other hand it can be killed with cinnamon essential oil for the nematode invaded, Avermectin B2.Therefore, Avermectin B2 and cinnamon essential oil mixture, by combining treatment with prevention for nematode, it can not only significantly reduce dosage, improve protection effect, can also reduce spraying times, significantly reduce a mu dispenser cost.
Description
Technical field
The invention belongs to pesticide field, and in particular to a kind of agricultural chemicals and its application for being used to prevent and treat nematode.
Background technology
Nematode is a kind of omnivorousness phytopathy protozoon of height specialized form, throughout grain, economy, vegetables, fruit tree, flowers,
The crops such as weeds, the especially harm to plants such as Solanaceae, Curcurbitaceae, Cruciferae are very serious, after disease occurs, can cause to make
Thing underproduction 10-20%, up to more than 75%, or even total crop failure when serious.Damaged every year caused by the harm of root-knot nematode in the world
Up to 100,000,000,000 dollars are lost, annual about 3,000,000,000 dollars of the loss of China.
In traditional AVM production process (crystallization, purification), in order to obtain the Avermectin B1a of high-purity, AVM hereinafter
Rhzomorph B2 is to be separated and discard as impurity, but finds that Avermectin B2 also has higher virulence to nematode recently,
Medicament of the Avermectin B2 as preventing and treating nematode is developed, can be turned waste into wealth, mitigates the pressure of environmental pollution, improves Avermectin
The agricultural chemicals utilization rate of element.
Cinnamon essential oil is the aromatic oil extracted from cassia bark, cassia twig, osmanthus leaf, and main component is cinnamic acid, also cinnamyl alcohol,
The compositions such as cinnamyl acetate, salicylic acid, benzaldehyde, salicylide, eugenol, vanillic aldehyde.At present, cinnamon essential oil is mainly used in anti-
The fields such as corruption, antibacterial, and the research in terms of nematode is prevented and treated has no report.
The Main Means for preventing and treating nematode at this stage are to carry out soil treatment or pouring root, chemical agent (chlorine using chemical prevention
Change hardship, bromomethane, d-d mixture, Bromofume, dibromopropane and vapor Furadan, phonamiphos, Aldicarb and metham-sodium etc.) though
Right desinsection is fast, and effect is good, but has that toxicity is high, residual life is long, dosage is big, cost is high, people and other non-target organisms are easily made
Into the problems such as injury, and due to the continuous use of chemical agent, cause the resistance of nematode to strengthen, using effect reduces year by year.
Therefore, it is badly in need of developing efficient, less toxic, low dosage, the pharmaceutical preparations of the preventing and treating nematode of long lasting period.
The content of the invention
In view of the deficiencies in the prior art, present invention aims at a kind of agricultural chemicals of efficiently preventing and treating nematode is provided, to improve line
The prevention effect of worm, delay or overcome the resistance to the action of a drug of nematode, reduce the application times and cost of agricultural chemicals.
Agricultural chemicals provided by the present invention, its active component are made up of Avermectin B2 and cinnamon essential oil, the Avermectin
Plain B2 and cinnamon essential oil mass ratio are 1-5: 1-5, and remaining is auxiliary material.
Preferably, the nematode is root-knot nematode and cyst roundworm.
It is further preferred that the mass ratio of the Avermectin B2 and cinnamon essential oil is 3: 2.
It is further preferred that the mass concentration that the active component accounts for agricultural chemicals is 5~50%.
It is further preferred that the mass concentration that the active component accounts for agricultural chemicals is 10~30%, optimal is 20%.
Compared with prior art, the invention has the advantages that：
1st, Avermectin B2 and cinnamon essential oil mixture have a significant synergistic effect, wherein Avermectin B2 and cinnamon essential oil with
3:Synergy during 2 mixing is most notable, and its CTC reaches 161.35.Due to cinnamon essential oil have repellent, kill nematode dual work
With after the two compounding, one side cinnamon essential oil has repellent action to the nematode in soil, so as to significantly reduce nematode to making
Invasion, the harm of thing root；On the other hand it can be carried out with cinnamon essential oil for the nematode invaded, Avermectin B2
Kill.Therefore, Avermectin B2 and cinnamon essential oil mixture, combining treatment with prevention for nematode can not only significantly reduce
Dosage, protection effect is improved, can also reduce spraying times, significantly reduce a mu dispenser cost.
2nd, the present invention can delay or overcome the drug-fast appearance of nematode, extend the use of Avermectin B2 and cinnamon essential oil
Life-span.
3rd, the present invention has preferable prevention effect to root-knot nematode, cyst roundworm, and 120d is to tomato root-knot eelworm after medicine
Preventive effect be up to 90.69%, 30d is up to 87.60% to the preventive effect of soy bean cyst roundworm after medicine, is above comparison medicament 10%
Fosthiazate.
Specific embodiment
It is the specific embodiment of the present invention below, technical scheme is done and is further described, but it is of the invention
Protection domain be not limited to these embodiments.It is every to be included in this hair without departing substantially from the change of present inventive concept or equivalent substitute
Within bright protection domain.
Embodiment 1
By 10kg Avermectin B2s, 10kg cinnamon essential oils, 12kg methanol, 56.5kg dimethylbenzene, reactor is added to together
In, after being mixed evenly, 5kg phenethyl phenol polyethenoxy ethers are added, 6.5kg calcium dodecyl benzene sulfonates, are mixed equal
Even 2 hours, cream preparation is made.
Embodiment 2
By 10kg Avermectin B2s, 20kg cinnamon essential oils, 10kg methanol, 48kg dimethylbenzene, it is added to together in reactor,
After being mixed evenly, 6kg phenethyl phenol polyethenoxy ethers are added, 6.0kg calcium dodecyl benzene sulfonates, are mixed evenly 2
Hour, cream preparation is made.
Embodiment 3
By 5kg Avermectin B2s, 15kg cinnamon essential oils, 13kg methanol, 15kgDMF, 37.5kg cyclohexanone, it is added to together
In reactor, stir and be completely dissolved, by 6kg NPEs, 2kg epoxidized soybean oils, 5.5kg poly alkyl alcohols
Oxygen vinethene, 1kg Butacides, is added in aforesaid reaction vessel together, stirs and is completely dissolved, is slowly added into purified water,
It is stirring while adding, mix 2 hours, produce the microemulsion of the present invention.
Embodiment 4
By 10kg Avermectin B2s, 5kg cinnamon essential oils, 7kg methanol, 10kgDMF, 46.5kg cyclohexanone, it is added to together
In reactor, stir and be completely dissolved, by 8kg NPEs, 5kg epoxidized soybean oils, 6.5kg poly alkyl alcohols
Oxygen vinethene, 2kg Butacides, is added in aforesaid reaction vessel together, stirs and is completely dissolved, is slowly added into purified water,
It is stirring while adding, mix 2 hours, produce the microemulsion of the present invention.
Embodiment 5
By 10kg Avermectin B2s, 15kg cinnamon essential oils, 15kg methanol, 33.5kg dimethylbenzene, reactor is added to together
In, after being mixed evenly, 10kg phenethyl phenol polyethenoxy ethers are added, 16.5kg calcium dodecyl benzene sulfonates, are mixed
Uniform 2 hours, cream preparation is made.
Embodiment 6
By 9kg Avermectin B2s, 3kg cinnamon essential oils, 9.5kg methanol, 13kgDMF, 42kg cyclohexanone, it is added to together anti-
Answer in kettle, stir and be completely dissolved, by 10kg NPEs, 5kg epoxidized soybean oils, 6.5kg poly alkyl alcohols
Oxygen vinethene, 2kg Butacides, is added in aforesaid reaction vessel together, stirs and is completely dissolved, is slowly added into purified water,
It is stirring while adding, mix 2 hours, produce the microemulsion of the present invention.
Embodiment 7
By 12kg Avermectin B2s, 8kg cinnamon essential oils, 20kg methanol, 40kg dimethylbenzene, it is added to together in reactor,
After being mixed evenly, 6kg phenethyl phenol polyethenoxy ethers are added, 14kg calcium dodecyl benzene sulfonates, it is small to be mixed evenly 2
When, cream preparation is made.
Test example
1. cinnamon essential oil is tested the repellent effect of nematode
The sandy soil of sterilizing are put into nutritive cube (deep 10cm), by tomato root-knot eelworm, soy bean cyst roundworm, Sweet Potato line
Worm, 2 instar larvaes of aphelenchoides besseyi are inoculated in sandy soil (apart from basin bottom 3cm) respectively, every basin nematode about 400 or so.By meat
Osmanthus essential oil is made into 20% missible oil, then it is diluted to 200 ×, take 5ml to be used for pot experiment respectively, 5 points are injected per basin, medicament is used
Pipe stretches into sandy soil surface 2cm, by applied medicine basin alms bowl plastic membrane of polyvinyl chloride seal 7 days, open wide 4 days after, then transfer in
Each crop, percentage of injury is investigated after 30d, the results are shown in Table 1.
Repellent measurement result of the cinnamon essential oil of table 1 to nematode
| CK | Tomato root-knot eelworm | Soy bean cyst roundworm | Sweet potato stem nematode | Aphelenchoides besseyi | |
| Percentage of injury % | 42.5 | 1.5 | 4.4 | 26.5 | 29.6 |
As shown in Table 1, cinnamon essential oil has preferably stifling repellent action to tomato root-knot eelworm and soy bean cyst roundworm,
Can significantly reduce the percentage of injury of crop, and to sweet potato stem nematode, aphelenchoides besseyi to walk quickly and keep away effect poor.
Meanwhile investigate the tomato root-knot eelworm and soy bean cyst roundworm after different essential oil processing according to above identical method
Percentage of injury, it the results are shown in Table 2.
Table 2 different essential oils walk quickly and keep away Contrast on effect to tomato root-knot eelworm, soy bean cyst roundworm
| Processing | Tomato root-knot eelworm | Soy bean cyst roundworm |
| Cinnamon essential oil | 1.5 | 4.4 |
| Peppermint essential oil | 35.7 | 38.9 |
| Clove oil | 32.3 | 29.6 |
| Tea tree ethereal oil | 25.7 | 38.2 |
| Oil of Cymbopogon winterianus | 37.3 | 35.8 |
Result above shows that cinnamon essential oil has to tomato root-knot eelworm, soy bean cyst roundworm walks quickly and keeps away effect well, makees
Thing percentage of injury is very low, and other essential oils walk quickly and keep away poor effect.
2. Avermectin B2 and the toxicity test after cinnamon essential oil compounding
Choose embodiment 1-7 and indoor measurement, 2 instar larvae collection sides have been carried out to the killing action of the instar larvae of root-knot nematode 2
Method：(picking up from Wuhan Huang Po Wu Hu farms tomato greenhouse) from aggrieved tomato root knot, the fresh egg capsule of picking is placed on filter paper, will
Filter paper is placed on 75 μm of mesh screens, then mesh screen is placed in the culture dish for filling clear water, water is slightly covered pieces of an egg, 25 DEG C in incubator
Hatching, 24h collect 2 instar larvaes in culture dish.
Sod cultivation is the same as the activity determination method in embodiment 2.
Calculate the co-toxicity coefficient CTC of mixture according to the abundant methods of Sun Yun, the performance synergy of CTC >=120 mixed agent, the table of CTC≤80
It is now antagonism, 80 ﹤ CTC ﹤ 120 are summation action.Co-toxicity coefficient CTC calculates according to formula (1), (2), (3), the results are shown in Table 3.
ATI=S*100/M ... ... ... ... ... ... ... ... ... ... (1)
In formula：The toxicity index of ATI-mixture actual measurement；The EC of S-standard agent50, unit is milligrams per liter (mg/L)；
The EC of M-mixture50, unit is milligrams per liter (mg/L).
TTI=TIA*PA+TIB*PB………………………………………………(2)
In formula：The theoretical toxicity index of TTI-mixture；TIA- A medicament toxicity index；PA- A medicaments in mixture hundred
Divide content, unit is percentage %；TIB- B medicament toxicity index；PBPercentage composition of-B the medicaments in mixture, unit hundred
Divide rate %.
CTC=ATI*100/TTI ... ... ... ... ... ... ... ... ... (3)
In formula：CTC-co-toxicity coefficient；ATI-mixture actual measurement toxicity index；TTI-mixture theoretical toxicity index.
Toxicity Determination result of the medicament of table 3 to the instar larvae of root-knot nematode 2
As shown in Table 3, when Avermectin B2 is mixed with cinnamon essential oil with different ratio, the instar larvae of root-knot nematode 2 is killed
There is synergistic effect, CTC is all higher than 120, and wherein Avermectin B2 and cinnamon essential oil is with 3:Co-toxicity coefficient CTC during 2 mixing is most
Greatly 161.35.
3. the present invention is to the field controling test of root-knot nematode
Field controling test is carried out to the root-knot nematode of tomato from embodiment 7, comparison medicament is 10% fosthiazate
Granula (Japanese Ishihara Sangyo Kaisha, Ltd.'s production), experiment sets 5 processing altogether：The setting of embodiment 7 100ga.i./mu,
150ga.i./mu, 200ga.i./mu；10% fosthiazate granule 200ga.i./mu and blank control (clear water), each handle 4
Secondary repetition, plot area 30m2, random district's groups arrangement, isolation strip is set between each cell and processing, experimental field surrounding sets guarantor
Shield row, experimental field each cell fertilising and field management are horizontal consistent, and disabling is similar with Avermectin B2 during whole experiment
Medicament.Three of embodiment 7 handle insecticide-applying way：Pouring root after transplanting, every plant of decoction 300ml, fosthiazate granule is in tomato
Dispenser is spread fertilizer over the fields during transplanting.
The quantity of the instar larvae of root-knot nematode 2 (J2) in soil is detected when 30d, 60d, 90d after administration, is taken per 5 points of cell
Sample, every takes 5~20cm depth rhizosphere soils, and the 200g soil samples graceful funnel method separation of shellfish wherein root is taken after 5 soil samples are well mixed
The instar larvae of tie lines worm 2 simultaneously counts.120d sets the tone per solid point in each 5 points of samplings of cell diagonal and looks into 2 plants, investigate 10 altogether after dispenser
Strain, the corresponding strain number of record morbidity sum of series, then calculate root knot nematode disease disease index and compared with blank control cell, calculate
Prevention effect, it the results are shown in Table 4.
Diseased plant grade scale：0 grade be root system without insect gall；1 grade is that root system has a small amount of small insect gall；3 grades be 2/3 root system be covered with it is small
Insect gall；5 grades are that root system is covered with small insect gall and has secondary insect gall；7 grades are that root system forms fibrous root group.
Prevention effect of 4 medicament of the present invention of table to root-knot nematode
As shown in Table 4,90.69% is up to the preventive effect of the instar larvae of root-knot nematode 2 using 90d after dispenser of the present invention, shown
Work is better than the fosthiazate of comparison medicament 10%, the 120d investigation tomatoes root extent of injury after dispenser, 150ga.i./mu of embodiment 7,
The effect of 200ga.i./mu processing is still better than comparison medicament, and after 120d preventive effect is compared with medicine after the fosthiazate medicine of comparison medicament 10%
90d has started to be remarkably decreased, and illustrates that the lasting period of invention formulation is longer, better than 10% fosthiazate.
4. the present invention is to the field controling test of packing nematode
Field controling test has been carried out to soybean packing nematode from embodiment 7, embodiment 7 sets 100ga.i./mu,
150ga.i./mu, the processing of three kinds of concentration of 200ga.i./mu, comparison medicament be 10% lythidathion granule set 200ga.i./
Mu, if not dispenser blank control (CK), 3 repetitions of every kind of processing, random alignment.Insecticide-applying way is：Even rear ridging, ridge are harrowed into ground
4 ditches are outputed in face, by the decoction of embodiment 7 and the fresh water spraying of blank control in ditch, the lythidathion of comparison medicament 10%
Granula is spread fertilizer over the fields in ditch；Then soya seeds are sown in the ditch for having applied medicament, last earthing is simultaneously treaded, next day watering.Apply
30d is sampled investigation after medicine, and each cell takes 10 plants at random, records individual plant sporangiocyst number, and carries out statistical analysis, calculates anti-
Effect, the results are shown in Table 5.
Prevention effect of 5 medicament of the present invention of table to cyst roundworm
As shown in Table 5, the present invention has preferable prevention effect to cyst roundworm, and highest prevention effect is 87.60%, significantly
Better than 10% fosthiazate.
Claims (6)
- A kind of 1. agricultural chemicals for being used to prevent and treat nematode, it is characterised in that：The active component of the agricultural chemicals is by Avermectin B2 and cinnamon spirit Oil composition, the mass ratio of the Avermectin B2 and cinnamon essential oil is 1-5: 1-5.
- 2. it is used for the agricultural chemicals for preventing and treating nematode as claimed in claim 1, it is characterised in that：The nematode is root-knot nematode and sporangiocyst line Worm.
- 3. it is used for the agricultural chemicals for preventing and treating nematode as claimed in claim 1, it is characterised in that：The Avermectin B2 and cinnamon essential oil Mass ratio is 3: 2.
- 4. it is used for the agricultural chemicals for preventing and treating nematode as claimed in claim 1, it is characterised in that：The quality that the active component accounts for agricultural chemicals is dense Spend for 5~50%.
- 5. it is used for the agricultural chemicals for preventing and treating nematode as claimed in claim 1, it is characterised in that：The quality that the active component accounts for agricultural chemicals is dense Spend for 10~30%.
- 6. application of the agricultural chemicals in preventing and treating crop root-knot nematode and cyst roundworm described in claim 1-5 any one.
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| CN201710418606.0A CN107372644B (en) | 2017-06-06 | 2017-06-06 | It is a kind of for preventing and treating the pesticide of nematode |
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| CN201710418606.0A CN107372644B (en) | 2017-06-06 | 2017-06-06 | It is a kind of for preventing and treating the pesticide of nematode |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576068A (en) * | 2018-06-27 | 2018-09-28 | 河北昊阳化工有限公司 | A kind of mixture composition for preventing below pest and nematode |
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2017
- 2017-06-06 CN CN201710418606.0A patent/CN107372644B/en active Active
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| CN106172444A (en) * | 2016-07-05 | 2016-12-07 | 广东中迅农科股份有限公司 | Composition pesticide containing Avermectin B2 |
| CN106490046A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of prevent and treat agricultural chemicals of root knot nematode disease and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108576068A (en) * | 2018-06-27 | 2018-09-28 | 河北昊阳化工有限公司 | A kind of mixture composition for preventing below pest and nematode |
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