CN107372097A - Chinese yew artificial hybridization method - Google Patents
Chinese yew artificial hybridization method Download PDFInfo
- Publication number
- CN107372097A CN107372097A CN201710708631.2A CN201710708631A CN107372097A CN 107372097 A CN107372097 A CN 107372097A CN 201710708631 A CN201710708631 A CN 201710708631A CN 107372097 A CN107372097 A CN 107372097A
- Authority
- CN
- China
- Prior art keywords
- pollen
- sprayer
- time
- borax
- chinese yew
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of Chinese yew artificial hybridization method, it is characterized in that, comprise the following steps：（1）Pollen collection：When Taxus x media female plant is in full-bloom stage, the collection southerm yew male flower bloomed of the same day；（2）Processing method：Male flower is thinly spread out on blank sheet of paper, upper cover blank sheet of paper, is put under sunshine and shines, and every 2~2.5 h is turned over 1 time, 1 d is visible powder, with the light sieve of sieve, removes impurity removing；By pollen first with clear water by weight pollen：Water=1:1000~2000 ratio is modulated into pollen liquid, and chitosan, borax and sugar, addition are then added in pollen liquid is by weight：Chitosan 0.1~0.3%, borax 3~5%, sugar 5~10%；（3）Sprayed and hybridized using sprayer, sprayed once using sprayer every 3~4 days, cohybridization 3 times.The characteristic for the existing southerm yew fast-growth of filial generation that Chinese yew artificial hybridization method of the present invention obtains, there is the cold-resistant and drought resisting advantage of Taxus x media again.
Description
Technical field
The present invention relates to a kind of Chinese yew artificial hybridization method, belong to planting technical field.
Background technology
Taxus in dioecious plant, due to Chinese yew genus plants it is not of the same race between male flower florescence it is different, and
The male and female plant florescence of Chinese yew of the same race is asynchronous, causes hybridization difficult, has had a strong impact on that the crossbreeding of Chinese yew genus plants is entered
Journey.
Exploitation and utilization and extention of the crossbreeding to Chinese yew all have very important meaning.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, there is provided a kind of Chinese yew artificial hybridization method, it is miscellaneous
The characteristic of the existing southerm yew fast-growth of offspring is handed over, the cold-resistant and drought resisting advantage with Taxus x media, is shown
Stronger hybrid vigour, has a good application prospect.
According to technical scheme provided by the invention, the Chinese yew artificial hybridization method, it is characterized in that, including following step
Suddenly：
（1）Pollen collection：When Taxus x media female plant is in full-bloom stage, the collection southerm yew male flower bloomed of the same day；
（2）Processing method：
Male flower is thinly spread out on blank sheet of paper, upper cover blank sheet of paper, is put under sunshine and shines, and every 2~2.5 h is turned over 1 time, 1 d is
It can be seen that powder, with the light sieve of sieve, removes impurity removing；By pollen first with clear water by weight pollen：Water=1:1000~2000 ratio modulation
Into pollen liquid, chitosan, borax and sugar, addition are then added in pollen liquid is by weight：Chitosan 0.1~0.3%,
Borax 3~5%, sugar 5~10%；
（3）Sprayed and hybridized using sprayer, sprayed once using sprayer every 3~4 days, cohybridization 3 times.
Further, the step（3）In, the time for hybridization of spraying just does rear or afternoon 15 for morning dew：00 or so.
Further, in addition to after hybridization manage：After 20d sprayer is used with concentration for 0.3~0.5% potassium dihydrogen phosphate
Foliage dressing 1 time, sprayed 1 time with 4~5% boraxs sprayer blade face after 30 d, with 0.3~0.5% potassium dihydrogen phosphate use after 50 d
Sprayer foliage dressing 1 time, with 0.3~0.5% borax sprayer foliage dressing 1 time after 70 d, with 0.3~0.5% after 90 d
Potassium dihydrogen phosphate sprayer foliage dressing 1 time.
Chinese yew artificial hybridization method of the present invention, hybridized using southerm yew pollen to Taxus x media, production
The method of raw cenospecies, the existing southerm yew fast-growth characteristic of filial generation, again with Taxus x media cold-resistant and
Drought resisting advantage, stronger hybrid vigour is shown, there should be prospect with good.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment one：A kind of Chinese yew artificial hybridization method, comprises the following steps：
（1）Pollen collection：
When Taxus x media female plant is in full-bloom stage, the collection southerm yew male flower bloomed of the same day；
（2）Processing method：
Male flower is thinly spread out on blank sheet of paper, upper cover blank sheet of paper, is put under sunshine and shines, and every 2 h is turned over 1 time, 1 d is visible
Powder, with the light sieve of sieve, remove impurity removing；By pollen first with clear water by weight pollen：Water=1:1000 ratio is modulated into pollen liquid,
Then chitosan, borax and sugar, addition are added in pollen liquid is by weight：Chitosan 0.1%, borax 3%, sugar 5%, with
Ensure the vigor of pollen, improve hybridization success rate；
（3）Sprayed and hybridized using sprayer, the optimal hybridization time of Taxus x media female plant is after morning dew is just done or afternoon
15：00 or so, sprayed once using sprayer every 3~4 days, cohybridization 3 times；Hybridization touches female chapiter with finger, bright
Showing has hairline wire water band, and crossbreeding effect is best；
（4）Managed after hybridization：
With concentration it is 0.3% potassium dihydrogen phosphate using sprayer foliage dressing 1 time after 20d, with 4% borax sprayer leaf after 30 d
Face is sprayed 1 time, with 0.3% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 50 d, with 0.3% borax sprayer leaf after 70 d
Face is applied fertilizer 1 time, with 0.3% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 90 d；After so handling, pollen can be strengthened
Activity, rate of fertilization is improved, with average result rate after sprayer spraying hybridization up to 97%.
Embodiment two：A kind of Chinese yew artificial hybridization method, comprises the following steps：
（1）Pollen collection：
When Taxus x media female plant is in full-bloom stage, the collection southerm yew male flower bloomed of the same day；
（2）Processing method：
Male flower is thinly spread out on blank sheet of paper, upper cover blank sheet of paper, is put under sunshine and shines, and every 2.5 h is turned over 1 time, 1 d is visible
Powder, with the light sieve of sieve, remove impurity removing；By pollen first with clear water by weight pollen：Water=1:2000 ratio is modulated into pollen liquid,
Then chitosan, borax and sugar, addition are added in pollen liquid is by weight：Chitosan 0.3%, borax 5%, sugar 10%, with
Ensure the vigor of pollen, improve hybridization success rate；
（3）Sprayed and hybridized using sprayer, the optimal hybridization time of Taxus x media female plant is after morning dew is just done or afternoon
15：00 or so, sprayed once using sprayer every 4 days, cohybridization 3 times；Hybridization touches female chapiter with finger, hence it is evident that has
Hairline wire water band, crossbreeding effect are best；
（4）Managed after hybridization：
With concentration it is 0.5% potassium dihydrogen phosphate using sprayer foliage dressing 1 time after 20d, with 5% borax sprayer leaf after 30 d
Face is sprayed 1 time, with 0.5% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 50 d, with 0.5% borax sprayer leaf after 70 d
Face is applied fertilizer 1 time, with 0.5% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 90 d；After so handling, pollen can be strengthened
Activity, rate of fertilization is improved, with average result rate after sprayer spraying hybridization up to 97%.
Embodiment three：A kind of Chinese yew artificial hybridization method, comprises the following steps：
（1）Pollen collection：
When Taxus x media female plant is in full-bloom stage, the collection southerm yew male flower bloomed of the same day；
（2）Processing method：
Male flower is thinly spread out on blank sheet of paper, upper cover blank sheet of paper, is put under sunshine and shines, and every 2 h is turned over 1 time, 1 d is visible
Powder, with the light sieve of sieve, remove impurity removing；By pollen first with clear water by weight pollen：Water=1:1500 ratio is modulated into pollen liquid,
Then chitosan, borax and sugar, addition are added in pollen liquid is by weight：Chitosan 0.2%, borax 4%, sugar 6%, with
Ensure the vigor of pollen, improve hybridization success rate；
（3）Sprayed and hybridized using sprayer, the optimal hybridization time of Taxus x media female plant is after morning dew is just done or afternoon
15：00 or so, sprayed once using sprayer every 3 days, cohybridization 3 times；Hybridization touches female chapiter with finger, hence it is evident that has
Hairline wire water band, crossbreeding effect are best；
（4）Managed after hybridization：
With concentration it is 0.4% potassium dihydrogen phosphate using sprayer foliage dressing 1 time after 20d, with 4.5% borax sprayer after 30 d
Blade face is sprayed 1 time, with 0.4% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 50 d, with 0.4% borax sprayer after 70 d
Foliage dressing 1 time, with 0.4% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 90 d；After so handling, flower can be strengthened
Powder activity, improves rate of fertilization, with average result rate after sprayer spraying hybridization up to 97%.
Claims (3)
1. a kind of Chinese yew artificial hybridization method, it is characterized in that, comprise the following steps：
（1）Pollen collection：When Taxus x media female plant is in full-bloom stage, the collection southerm yew male flower bloomed of the same day；
（2）Processing method：
Male flower is thinly spread out on blank sheet of paper, upper cover blank sheet of paper, is put under sunshine and shines, and every 2~2.5 h is turned over 1 time, 1 d is
It can be seen that powder, with the light sieve of sieve, removes impurity removing；By pollen first with clear water by weight pollen：Water=1:1000~2000 ratio modulation
Into pollen liquid, chitosan, borax and sugar, addition are then added in pollen liquid is by weight：Chitosan 0.1~0.3%,
Borax 3~5%, sugar 5~10%；
（3）Sprayed and hybridized using sprayer, sprayed once using sprayer every 3~4 days, cohybridization 3 times.
2. Chinese yew artificial hybridization method as claimed in claim 1, it is characterized in that：The step（3）In, hybridization of spraying when
Between for morning dew it is just dry after or afternoon 15：00 or so.
3. Chinese yew artificial hybridization method as claimed in claim 1, it is characterized in that：Managed after also including hybridization：With dense after 20d
It is 0.3~0.5% potassium dihydrogen phosphate using sprayer foliage dressing 1 time to spend, with 4~5% boraxs sprayer blade face spray 1 after 30 d
It is secondary, with 0.3~0.5% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 50 d, sprayed after 70 d with 0.3~0.5% borax
Day with fog foliage dressing 1 time, with 0.3~0.5% potassium dihydrogen phosphate sprayer foliage dressing 1 time after 90 d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710708631.2A CN107372097A (en) | 2017-08-17 | 2017-08-17 | Chinese yew artificial hybridization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710708631.2A CN107372097A (en) | 2017-08-17 | 2017-08-17 | Chinese yew artificial hybridization method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107372097A true CN107372097A (en) | 2017-11-24 |
Family
ID=60352624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710708631.2A Pending CN107372097A (en) | 2017-08-17 | 2017-08-17 | Chinese yew artificial hybridization method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107372097A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477974A (en) * | 2013-10-09 | 2014-01-01 | 四川地珍生物生态科技有限公司 | Method for promoting fruit bearing of Taxus media |
| CN105519430A (en) * | 2016-02-22 | 2016-04-27 | 杭州富阳飞博科技有限公司 | Genetic breeding method for Taxus media |
| CN105638436A (en) * | 2016-01-10 | 2016-06-08 | 江苏省中国科学院植物研究所 | Hybridization breeding method of taxus chinensis |
-
2017
- 2017-08-17 CN CN201710708631.2A patent/CN107372097A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477974A (en) * | 2013-10-09 | 2014-01-01 | 四川地珍生物生态科技有限公司 | Method for promoting fruit bearing of Taxus media |
| CN105638436A (en) * | 2016-01-10 | 2016-06-08 | 江苏省中国科学院植物研究所 | Hybridization breeding method of taxus chinensis |
| CN105519430A (en) * | 2016-02-22 | 2016-04-27 | 杭州富阳飞博科技有限公司 | Genetic breeding method for Taxus media |
Non-Patent Citations (1)
| Title |
|---|
| 梁朔等: "杂种优势在红豆杉上的应用研究", 《现代农业科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106146184A (en) | Fertilizer | |
| CN102283008A (en) | Cultivation method for interplanting white tea tree and pear tree | |
| CN104221798B (en) | Fattening method for peach tree in growing period | |
| CN103329772A (en) | Method for prolonging abscission period of ginkgo leaves | |
| CN107960265A (en) | A kind of method of flat Europe hybrid hazel layering seedling growth | |
| CN105210735A (en) | A kind of prevention and controls of Chlorotic disorder of peach tree | |
| CN105104136A (en) | Moisture and illumination managing method for promoting growth of tillandsia | |
| CN106954505A (en) | It is a kind of to improve loquat percentage of fertile fruit and the cultural method of quality | |
| CN105900786A (en) | Method for managing adult grape vines | |
| CN105191765A (en) | Moisture and light illumination management method for accelerating growth of tillandsia by combining application of magnesium fertilizer | |
| CN103141357A (en) | Method for preventing loquat fruits from splitting | |
| CN103477974A (en) | Method for promoting fruit bearing of Taxus media | |
| CN107372097A (en) | Chinese yew artificial hybridization method | |
| CN104335787A (en) | Cultivation method for land-cultivated oil chestnuts | |
| CN107258453A (en) | A kind of southern white poplar plum implantation methods | |
| CN107182527A (en) | A kind of garderning green maintenance process | |
| CN106146183A (en) | Ipomoea batatas fertilizer additive | |
| CN105347879A (en) | Fertilizing method for blueberry planting | |
| CN204180526U (en) | A kind of micro-spray device that can apply fertilizer | |
| CN204392842U (en) | A kind of screening mouth slight irrigation spray band | |
| CN104387212A (en) | Utilization-improved water soluble fertilizer | |
| CN110128187A (en) | A kind of plant rooting and rejuvenating fertilizer | |
| CN104838943A (en) | Maidenhair tree wart preventing method | |
| CN108029427A (en) | A kind of oil tea planting technology | |
| CN107593737A (en) | It is a kind of to postpone the ripe growth regulator of blueberry and its application method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171124 |