CN107371805A - A kind of flat mushroom greenhouse bacterium germination method - Google Patents

A kind of flat mushroom greenhouse bacterium germination method Download PDF

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Publication number
CN107371805A
CN107371805A CN201710706981.5A CN201710706981A CN107371805A CN 107371805 A CN107371805 A CN 107371805A CN 201710706981 A CN201710706981 A CN 201710706981A CN 107371805 A CN107371805 A CN 107371805A
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CN
China
Prior art keywords
bacterium
bacterium bag
greenhouse
flat mushroom
bag
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CN201710706981.5A
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Chinese (zh)
Inventor
侯新龙
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Oasis Of Guangxi County Of Liucheng Ecological Agriculture Technology Co Ltd
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Oasis Of Guangxi County Of Liucheng Ecological Agriculture Technology Co Ltd
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Priority to CN201710706981.5A priority Critical patent/CN107371805A/en
Publication of CN107371805A publication Critical patent/CN107371805A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The invention discloses a kind of flat mushroom greenhouse bacterium germination method, methods described comprises the following steps:(1) bacterium is connect:After oyster mushroom culture medium pack, punched with club on packed culture medium, flat mushroom strain is then accessed into hole, obtains bacterium bag;(2) stacking:In mushroom cultivation greenhouse, the bacterium bag after step (1) is inoculated with carries out stacking, and space is left between bacterium pile;(3) collapse of setting:During bacterium germination, collapse of setting is carried out when the bacterium pile intermediate layer of step (2) or bottom bacterium bag temperature reach 33 35 DEG C;(4) flat mushroom bacterium germination is carried out with above-mentioned steps (1) (3), bacterium germination can be completed after 20 25 days.This method uses fermentation medium, and by controlling the temperature, humidity and bacterium bag temperature in greenhouse to promote mycelial growth, the flat mushroom bacterium germination time foreshortens to 20 25 days.

Description

A kind of flat mushroom greenhouse bacterium germination method
Technical field
The invention belongs to edible mushroom technical field, in particular it relates to a kind of flat mushroom greenhouse bacterium germination method.
Background technology
Flat mushroom, also known as pick up the ears, oyster cap fungus, oyster mushroom, black tree peony mushroom, TaiWan, China is also known as elegant precious mushroom, is under Basidiomycota The species of Agaricales Pleurotaceae one, it is that kind of a fairly common grey eats mushroom.
The continuous branch elongation of flat mushroom germ tube, forms uninucleate hyphae.After the different uninucleate hyphae of sex combines, double-core bacterium is formed Silk.Nucleated mycelium has clamp connection on barrier film.Nucleated mycelium constantly carries out cell division, produces and divide by means of clamp connection Branch, under conditions of environment is suitable, ad infinitum carries out growth and breeding.
In hymenium, nucleated mycelium top produces load, and its inhereditary material is recombinated and separated, and forms four load spores Son.After spore maturation, ejected from lamella, complete a life cycle.
The traditional Chinese medical science thinks that flat mushroom is warm-natured, sweet, has the effect of wind dispelling cold dispelling, relaxing tendons and activating collaterals.For controlling lumbocrural pain, brothers The numb, illness such as grain is obstructed.Proteoglycan body in flat mushroom has very strong inhibitory action to cancer cell, can strengthen immunity of organism Function.
Bacterium germination, edible fungus culturing technics, also known as " walking bacterium ", refer to the process of mycelium growth, diffusion in culture substratess. Bacterium germination will pass through field planting, front cover, wear the processes such as bottom, and " field planting " is also known as " material feeding ", refer to inoculation ferfas silk and grow again in new culture In substratess;" front cover ", refer to after planting mycelium and cover with compost surface;" wearing bottom ", refer to mycelium in compost from top to bottom It is saturating to cover with hair.
Flat mushroom bacterium germination is carried out typically in greenhouse, and the current flat mushroom bacterium germination time, the time was partially long typically at 25-30 days.Such as Fruit can shorten the bacterium germination time, have practical significance to mushroom cultivation.
The content of the invention
The invention aims to solve the above problems, there is provided a kind of flat mushroom greenhouse bacterium germination method.
The purpose of the present invention implements by the following technical programs:
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:After oyster mushroom culture medium pack, punched on packed culture medium with club, then connect into hole Enter flat mushroom strain, obtain bacterium bag;
(2) stacking:In mushroom cultivation greenhouse, the bacterium bag after step (1) is inoculated with carries out stacking, and sky is left between bacterium pile Gap;
(3) collapse of setting:During bacterium germination, enter when the bacterium pile intermediate layer of step (2) or bottom bacterium bag temperature reach 33-35 DEG C Row collapse of setting;
(4) flat mushroom bacterium germination is carried out with above-mentioned steps (1)-(3), bacterium germination can be completed after 20-25 days.
Further, in the step (1), the oyster mushroom culture medium is made using fermentation method, and punching depth is 10- 12cm。
Further, in the step (2), temperature control in greenhouse is at 25-27 DEG C, and humid control is in 68-73%.
Further, temperature control in greenhouse is at 26 DEG C, and humid control is 70%.
Further, in the step (2), the bacterium bag number of plies is layer 2-4 in bacterium pile, every layer of 6-8 bacterium bag.
Further, the bacterium bag number of plies is 4 layers in bacterium pile, every layer of 7 bacterium bag.
Further, in the step (2), the space between bacterium pile is 60-70cm.
Further, in the step (3), the collapse of setting method is:When the bacterium bag number of plies is 2 layers, by upper strata and bottom Bacterium bag location swap, bacterium bag rotate 180 °.
Further, in the step (3), when the bacterium bag number of plies is 3 layers, upper strata bacterium bag is put into intermediate layer, intermediate layer Bacterium bag is put into bottom, and bottom bacterium bag rotates 180 ° and is put into upper strata, or, bottom bacterium bag is put into intermediate layer, intermediate layer bacterium bag is put To upper strata, upper strata bacterium bag rotates 180 ° and is put into bottom.
Further, in the step (3), when the bacterium bag number of plies is 4 layers, intermediate layer bacterium bag is put into upper strata or bottom, The bacterium bag of upper strata and bottom is put into intermediate layer.
Oyster mushroom culture medium of the present invention is made using fermentation method, and bacterium germination is more easy to than raw material or clinker;Greenhouse temperature control during bacterium germination Make at 25-27 DEG C, slightly above the temperature of prior art, control the rational number of plies of bacterium pile and every layer of bacterium bag number, strictly control bacterium Bag temperature, prevention occur to burn bacterium, may advantageously facilitate Growth of Pleurotus Mycelium.
The invention has the advantages that:
Flat mushroom greenhouse bacterium germination method provided by the invention, using fermentation medium, by control the temperature in greenhouse, humidity and Bacterium bag temperature promotes mycelial growth, and the flat mushroom bacterium germination time foreshortens to 20-25 days.
Embodiment
Embodiment 1
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:Common oyster mushroom culture medium made from fermentation is packed, punched with sharp rod on packed culture medium, Depth is punched in 10-12cm, flat mushroom strain is then accessed into hole, obtains bacterium bag.
(2) stacking:26 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 70%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 4 layers of bacterium bag, every layer of 7 bacterium bag, and 70cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bacterium bag temperature reaches 33-35 DEG C when intermediate layer, by intermediate layer bacterium bag It is put into upper strata or bottom, the bacterium bag of upper strata and bottom is put into intermediate layer.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 20 days.
Embodiment 2
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:Common oyster mushroom culture medium made from fermentation is packed, punched with sharp rod on packed culture medium, Depth is punched in 10-12cm, flat mushroom strain is then accessed into hole, obtains bacterium bag.
(2) stacking:25 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 68%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 3 layers of bacterium bag, every layer of 8 bacterium bag, and 60cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bacterium bag temperature reaches 33-35 DEG C when intermediate layer, upper strata bacterium bag is put To intermediate layer, intermediate layer bacterium bag is put into bottom, and bottom bacterium bag rotates 180 ° and is put into upper strata.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 25 days.
Embodiment 3
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:Common oyster mushroom culture medium made from fermentation is packed, punched with sharp rod on packed culture medium, Depth is punched in 10-12cm, flat mushroom strain is then accessed into hole, obtains bacterium bag.
(2) stacking:27 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 73%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 2 layers of bacterium bag, every layer of 6 bacterium bag, and 70cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bottom bacterium bag temperature reaches 33-35 DEG C, by upper strata and bottom Bacterium bag location swap, bacterium bag rotate 180 °.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 22 days.
Embodiment 4
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:Common oyster mushroom culture medium made from fermentation is packed, punched with sharp rod on packed culture medium, Depth is punched in 10-12cm, flat mushroom strain is then accessed into hole, obtains bacterium bag.
(2) stacking:25 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 68%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 2 layers of bacterium bag, every layer of 6 bacterium bag, and 60cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bottom bacterium bag temperature reaches 33-35 DEG C, by upper strata and bottom Bacterium bag location swap, bacterium bag rotate 180 °.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 22 days.
Embodiment 5
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:Common oyster mushroom culture medium made from fermentation is packed, punched with sharp rod on packed culture medium, Depth is punched in 10-12cm, flat mushroom strain is then accessed into hole, obtains bacterium bag.
(2) stacking:27 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 73%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 4 layers of bacterium bag, every layer of 8 bacterium bag, and 70cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bacterium bag temperature reaches 33-35 DEG C when intermediate layer, by intermediate layer bacterium bag It is put into upper strata or bottom, the bacterium bag of upper strata and bottom is put into intermediate layer.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 21 days.
Comparative example 1
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:The common raw material oyster mushroom culture medium pack of identical will be formed with embodiment 1, with sharp rod in packed culture Punched on base, then punching depth accesses flat mushroom strain into hole, obtains bacterium bag in 10-12cm.
(2) stacking:26 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 70%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 4 layers of bacterium bag, every layer of 7 bacterium bag, and 70cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bacterium bag temperature reaches 33-35 DEG C when intermediate layer, by intermediate layer bacterium bag It is put into upper strata or bottom, the bacterium bag of upper strata and bottom is put into intermediate layer.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 28 days.
Comparative example 2
A kind of flat mushroom greenhouse bacterium germination method, methods described comprise the following steps:
(1) bacterium is connect:Raw material is formed into common raw material oyster mushroom culture medium same as Example 1 to pack, with sharp rod packed Punched on culture medium, then punching depth accesses flat mushroom strain into hole, obtains bacterium bag in 10-12cm.
(2) stacking:23 DEG C of the temperature in mushroom cultivation greenhouse is controlled, humidity 65%, the bacterium bag after inoculation is subjected to stacking, it is single Individual bacterium pile sets 4 layers of bacterium bag, every layer of 7 bacterium bag, and 70cm spaces are left between bacterium pile.
(3) collapse of setting:During bacterium germination, collapse of setting is carried out when bacterium bag temperature reaches 33-35 DEG C when intermediate layer, by intermediate layer bacterium bag It is put into upper strata or bottom, the bacterium bag of upper strata and bottom is put into intermediate layer.
(4) flat mushroom bacterium germination is carried out with above-mentioned steps, bacterium germination is completed after 26 days.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality Apply example the present invention is described in detail, for those skilled in the art, it still can be to foregoing each implementation Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic.All essences in the present invention God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.

Claims (10)

  1. A kind of 1. flat mushroom greenhouse bacterium germination method, it is characterised in that methods described comprises the following steps:
    (1) bacterium is connect:After oyster mushroom culture medium pack, punched, then accessed into hole flat on packed culture medium with club Mushroom strains, obtain bacterium bag;
    (2) stacking:In mushroom cultivation greenhouse, the bacterium bag after step (1) is inoculated with carries out stacking, and space is left between bacterium pile;
    (3) collapse of setting:During bacterium germination, fallen when the bacterium pile intermediate layer of step (2) or bottom bacterium bag temperature reach 33-35 DEG C Pile;
    (4) flat mushroom bacterium germination is carried out with above-mentioned steps (1)-(3), bacterium germination can be completed after 20-25 days.
  2. 2. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (1), the flat mushroom training Support base to be made using fermentation method, punching depth is 10-12cm.
  3. 3. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (2), greenhouse temperature control System is at 25-27 DEG C, and humid control is in 68-73%.
  4. 4. flat mushroom greenhouse bacterium germination method according to claim 3, it is characterised in that temperature control in greenhouse is at 26 DEG C, humidity Control is 70%.
  5. 5. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (2), bacterium bag in bacterium pile The number of plies is layer 2-4, every layer of 6-8 bacterium bag.
  6. 6. flat mushroom greenhouse bacterium germination method according to claim 5, it is characterised in that the bacterium bag number of plies is 4 layers, every layer in bacterium pile 7 bacterium bags.
  7. 7. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (2), between bacterium pile Space is 60-70cm.
  8. 8. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (3), the collapse of setting side Method is:When the bacterium bag number of plies is 2 layers, by upper strata and the bacterium bag location swap of bottom, bacterium bag rotates 180 °.
  9. 9. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (3), when the bacterium bag number of plies For 3 layers when, upper strata bacterium bag is put into intermediate layer, intermediate layer bacterium bag is put into bottom, and bottom bacterium bag rotates 180 ° and is put into upper strata, or Person, bottom bacterium bag is put into intermediate layer, intermediate layer bacterium bag is put into upper strata, and upper strata bacterium bag rotates 180 ° and is put into bottom.
  10. 10. flat mushroom greenhouse bacterium germination method according to claim 1, it is characterised in that in the step (3), when bacterium bag layer When number is 4 layers, intermediate layer bacterium bag is put into upper strata or bottom, the bacterium bag of upper strata and bottom is put into intermediate layer.
CN201710706981.5A 2017-08-17 2017-08-17 A kind of flat mushroom greenhouse bacterium germination method Pending CN107371805A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657031A (en) * 2012-05-24 2012-09-12 宁城县正元利科技有限责任公司 High-yield cultivation method of oyster mushroom by normal-temperature fermentation and vertical soil coverage
CN103348869A (en) * 2013-06-28 2013-10-16 广西壮族自治区林业科学研究院 Cultivation method of pleurotus citrinopileatus
CN105027986A (en) * 2015-08-25 2015-11-11 贵州茅台流域东升农场有限公司 Oyster mushroom indoor bag cultivation method
CN106718023A (en) * 2016-11-25 2017-05-31 河池市农业科学研究所 A kind of hair bacterium management method of flat mushroom

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657031A (en) * 2012-05-24 2012-09-12 宁城县正元利科技有限责任公司 High-yield cultivation method of oyster mushroom by normal-temperature fermentation and vertical soil coverage
CN103348869A (en) * 2013-06-28 2013-10-16 广西壮族自治区林业科学研究院 Cultivation method of pleurotus citrinopileatus
CN105027986A (en) * 2015-08-25 2015-11-11 贵州茅台流域东升农场有限公司 Oyster mushroom indoor bag cultivation method
CN106718023A (en) * 2016-11-25 2017-05-31 河池市农业科学研究所 A kind of hair bacterium management method of flat mushroom

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