CN107365822A - A kind of preparation method of olive growing leaves protein extract - Google Patents
A kind of preparation method of olive growing leaves protein extract Download PDFInfo
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- CN107365822A CN107365822A CN201710789239.5A CN201710789239A CN107365822A CN 107365822 A CN107365822 A CN 107365822A CN 201710789239 A CN201710789239 A CN 201710789239A CN 107365822 A CN107365822 A CN 107365822A
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- growing leaves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract
The invention discloses a kind of preparation method of olive growing leaves protein extract, this method is that efficiently extraction protein, mechanism cooperate with enzymolysis from olive growing leaves with microwave radiation technology enzyme process, accelerates desorption and the absorption of enzyme；The enzyme that the present invention uses is neutral proteinase, carried than traditional acid or destruction of the alkali carries method to amino acid is smaller, and reaction condition is more easy to reach, course of reaction is more controllable, sodium ascorbate is added in slurrying shattering process simultaneously as inhibitor, albuminous degeneration can effectively be prevented, in addition auximone solution is added in enzymolysis process as enzymolysis accelerator, the activity of protease can be effectively promoted, accelerate enzymolysis process, shorten extraction time, extraction efficiency is obviously improved, realize the high-valued processing of olive growing leaves, it is more suitable for large-scale industrial production application.
Description
Technical field
The present invention relates to technical field of bioseparation, more particularly to a kind of preparation method of olive growing leaves protein extract.
Background technology
Olive is a kind of famous seeds for having in the world long history, is described as the rare high value resource in the world.
Olive fruit core is small, meat is thick, oil-containing is more, is the non-defective unit for extracting olive oil, has higher use value.Relative to dilute
The olive oil of property is lacked, olive growing leaves yield is larger, and Potential Comprehensive Utilization is big, and contains abundant bioactivity in olive growing leaves
Composition, such as secoiridoid, flavonoids, sterol, alkanes and volatile ingredient, being related to the high-valued of olive growing leaves should
Concentrated with also main in the extraction of these active materials, and the leaf slag of remaining millions of tons is often handled as low value.Have
Research shows, containing for more protein, mineral matter and other nutritional ingredients, especially protein is contained in olive leaf slag
Amount up to 11% or so there is higher production application to be worth.But the application of leaf slag is related at present mainly by the system of fermenting
It is not high into animal feed, added value.
Vegetable protein has aboundresources, cheap, energy conversion efficiency is high, is practically free of cholesterol and saturated fat
The advantages that sour, it is increasingly becoming the main protein source of the mankind.The intensive processing technology of vegetable protein is studied, develops high added value
Product, there is good economic benefit.Extract the side of vegetable protein both at home and abroad at present
Method has：The methods of alkalinity extraction, Enzymatic Extraction, compound collecting, UF membrane extraction, ion exchange extraction, wherein enzyme process carries
Take therefore recovery rate be high, environmental friendly gently wide concerned,《Aqueous phase enzyme process extracts grinding for albumen from cold press olive pomace
Study carefully》Application of the hydrolysis enzyme process in the extraction of olea europaea fruit residue protein has been inquired into once text.We are not difficult to find out, although enzyme process carries
Many advantages are taken, but very strict are required to extraction conditions in actual applications, enzymolysis time is longer, pushing away in the industry
Extensively using limited.
The content of the invention
A kind of the defects of the object of the invention is exactly to make up prior art, there is provided preparation of olive growing leaves protein extract
Method.
The present invention is achieved by the following technical solutions：
A kind of preparation method of olive growing leaves protein extract, comprises the following steps：
（1）Fresh, nothing is gone bad, the olive growing leaves of free from admixture clean up rear and L-AA sodium, water according to 1g:0.01g：
10-15mL ratio mixed grinding crushes, and it is 7 that appropriate cushioning liquid regulation system pH value is added in obtained slurries, standby；
（2）To step（1）Neutral proteinase, auximone solution are added in the slurries of preparation, microwave device is placed in after mixing
In, the assistance enzymolysis 30-80min under the conditions of 40-50 DEG C, 200-500W, it is standby that processing obtains enzymolysis liquid after terminating；
（3）Rejection filter after obtained enzymolysis liquid enzyme deactivation is removed slag, it is standby to collect filtered fluid；
（4）With hydrochloric acid or lemon acid for adjusting pH value it is that 5-6 carries out the heavy processing of acid by obtained protein liquid, after floccule to appear
Centrifuge, it is standby to obtain albumen powder；
（5）Obtained albumen powder is freeze-dried, produces described olive growing leaves protein extract.
Described step（1）In cushioning liquid be pH value be 6.5-7 phosphoric acid, boric acid or Tris buffer solutions.
Described step（2）The ratio of middle neutral proteinase and slurries is 500-2000U/g.
Described step（2）The weight ratio of middle auximone and slurries is 1:100.
Described step（2）In auximone solution for heteroauxin, 4- chloro-indoles acetic acid, phenylacetic acid, α-naphthalene second
Acid, 2,6- dichlorobenzoic acids, indolebutyric acid, the aqueous solution of one or more compositions in 5- rutiace ethyl esters.
The concentration of described auximone solution is 0.1%-0.5%.
The present invention efficiently extracts protein, mechanism and enzymolysis phase with microwave radiation technology enzyme process from olive growing leaves
Mutually cooperation, accelerates desorption and the absorption of enzyme；The enzyme that the present invention uses carries or alkali carries side for neutral proteinase than traditional acid
Destruction of the method to amino acid is smaller, and reaction condition is more easy to reach, and course of reaction is more controllable, while is crushed in slurrying
Sodium ascorbate is added in journey as inhibitor, albuminous degeneration can be effectively prevented, add plant life in enzymolysis process in addition
Long plain solution can be effectively promoted the activity of protease, accelerate enzymolysis process, shorten extraction time, carry as enzymolysis accelerator
Take efficiency to be obviously improved, realize the high-valued processing of olive growing leaves, be more suitable for large-scale industrial production application.
Embodiment
A kind of preparation method of olive growing leaves protein extract, comprises the following steps：
（1）Fresh, nothing is gone bad, the olive growing leaves of free from admixture clean up rear and L-AA sodium, water according to 1g:0.01g：
10mL ratio mixed grinding crushes, and it is 7 that appropriate cushioning liquid regulation system pH value is added in obtained slurries, standby；
（2）To step（1）Neutral proteinase, auximone solution are added in the slurries of preparation, microwave device is placed in after mixing
In, the assistance enzymolysis 30min under the conditions of 40 DEG C, 200W, it is standby that processing obtains enzymolysis liquid after terminating；
（3）Rejection filter after obtained enzymolysis liquid enzyme deactivation is removed slag, it is standby to collect filtered fluid；
（4）With hydrochloric acid or lemon acid for adjusting pH value be 5 carries out that acid is heavy to be handled by obtained protein liquid, after floccule to appear from
The heart separates, and it is standby to obtain albumen powder；
（5）Obtained albumen powder is freeze-dried, produces described olive growing leaves protein extract.
Wherein step（1）In cushioning liquid be pH value be 6.5 phosphoric acid, boric acid or Tris buffer solutions.
Wherein step（2）The ratio of middle neutral proteinase and slurries is 500U/g, and the weight ratio of auximone and slurries is
1:100。
Described auximone solution is the heteroauxin aqueous solution that concentration is 0.1%.
Claims (6)
1. a kind of preparation method of olive growing leaves protein extract, it is characterised in that comprise the following steps：
（1）Fresh, nothing is gone bad, the olive growing leaves of free from admixture clean up rear and L-AA sodium, water according to 1g:0.01g：
10-15mL ratio mixed grinding crushes, and it is 7 that appropriate cushioning liquid regulation system pH value is added in obtained slurries, standby；
（2）To step（1）Neutral proteinase, auximone solution are added in the slurries of preparation, microwave device is placed in after mixing
In, the assistance enzymolysis 30-80min under the conditions of 40-50 DEG C, 200-500W, it is standby that processing obtains enzymolysis liquid after terminating；
（3）Rejection filter after obtained enzymolysis liquid enzyme deactivation is removed slag, it is standby to collect filtered fluid；
（4）With hydrochloric acid or lemon acid for adjusting pH value it is that 5-6 carries out the heavy processing of acid by obtained protein liquid, after floccule to appear
Centrifuge, it is standby to obtain albumen powder；
（5）Obtained albumen powder is freeze-dried, produces described olive growing leaves protein extract.
A kind of 2. preparation method of olive growing leaves protein extract as claimed in claim 1, it is characterised in that described step
（1）In cushioning liquid be pH value be 6.5-7 phosphoric acid, boric acid or Tris buffer solutions.
A kind of 3. preparation method of olive growing leaves protein extract as claimed in claim 1, it is characterised in that described step
（2）The ratio of middle neutral proteinase and slurries is 500-2000U/g.
A kind of 4. preparation method of olive growing leaves protein extract as claimed in claim 1, it is characterised in that described step
（2）The weight ratio of middle auximone and slurries is 1:100.
A kind of 5. preparation method of olive growing leaves protein extract as claimed in claim 1, it is characterised in that described step
（2）In auximone solution for heteroauxin, 4- chloro-indoles acetic acid, phenylacetic acid, α-naphthylacetic acid, 2,6- dichlorobenzoic acids,
The aqueous solution of indolebutyric acid, one or more compositions in 5- rutiace ethyl esters.
6. the preparation method of a kind of olive growing leaves protein extract as described in claim 1 or 4, it is characterised in that described
The concentration of auximone solution is 0.1%-0.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710789239.5A CN107365822A (en) | 2017-09-05 | 2017-09-05 | A kind of preparation method of olive growing leaves protein extract |
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| CN201710789239.5A CN107365822A (en) | 2017-09-05 | 2017-09-05 | A kind of preparation method of olive growing leaves protein extract |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101756175A (en) * | 2010-03-05 | 2010-06-30 | 中国农业科学院作物科学研究所 | Cassava leaf extract and preparation method and application thereof |
| CN103388018A (en) * | 2013-08-09 | 2013-11-13 | 青岛博研达工业技术研究所(普通合伙) | Method for preparing peanut protein special for fermentation by utilizing hot-pressed peanut meal |
| CN106390101A (en) * | 2016-09-18 | 2017-02-15 | 大连普瑞康生物技术有限公司 | Saussurea involucrate culture oral liquid with effect of clearing heat from throat and preparation method thereof |
| CN106822214A (en) * | 2015-12-03 | 2017-06-13 | 李飞燕 | A kind of Olive leaf P.E preparation method |
| CN106929353A (en) * | 2017-02-14 | 2017-07-07 | 广西肽王生物科技有限公司 | A kind of antidepressant Moringa peptide method for preparing medicated wine |
-
2017
- 2017-09-05 CN CN201710789239.5A patent/CN107365822A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101756175A (en) * | 2010-03-05 | 2010-06-30 | 中国农业科学院作物科学研究所 | Cassava leaf extract and preparation method and application thereof |
| CN103388018A (en) * | 2013-08-09 | 2013-11-13 | 青岛博研达工业技术研究所(普通合伙) | Method for preparing peanut protein special for fermentation by utilizing hot-pressed peanut meal |
| CN106822214A (en) * | 2015-12-03 | 2017-06-13 | 李飞燕 | A kind of Olive leaf P.E preparation method |
| CN106390101A (en) * | 2016-09-18 | 2017-02-15 | 大连普瑞康生物技术有限公司 | Saussurea involucrate culture oral liquid with effect of clearing heat from throat and preparation method thereof |
| CN106929353A (en) * | 2017-02-14 | 2017-07-07 | 广西肽王生物科技有限公司 | A kind of antidepressant Moringa peptide method for preparing medicated wine |
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Application publication date: 20171121 |
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