CN107362287B - Traditional Chinese medicine composition for treating cerebral ischemia and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for treating cerebral ischemia and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8988—Gastrodia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
Abstract
The invention discloses a traditional Chinese medicine composition for treating cerebral ischemia and a preparation method and application thereof. The effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 4-15 parts of gastrodia elata, 1-3 parts of trillium and 0.02-0.15 part of borneol. The traditional Chinese medicine composition has a remarkable effect of treating cerebral ischemia.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for treating cerebral ischemia, and also relates to a preparation method and application of the composition.
Background
Tianzhu powder is a brain-tonifying prescription of Tujia, consists of trillium and gastrodia elata according to the weight ratio of 1:2, has the effects of tonifying deficiency, strengthening body, tranquilizing and allaying excitement, dispelling wind and dispersing phlegm according to literature, and is used for treating dizziness in folk. Chinese patent application CN201210479479.2 discloses a compound Tianzhu powder, which is composed of 6g of parietal barberry (namely, trillium indicum) and 12g of gastrodia elata and is used for treating wind-phlegm type neurasthenia with symptoms of dizziness, insomnia, dreaminess, poor spirit, swollen tongue, white fur and slippery pulse.
Modern researches on Tianzhu powder show that Tianzhu powder has an improving effect on learning and memory disorders of rats caused by cerebral ischemia (Songkui et al, Tujia medicine Tianzhu powder has an improving effect on learning and memory disorders of rats caused by cerebral ischemia, Chinese herbal medicine [ J ], 2015 03 th, 389-395 pages); tianzhu powder also has an improving effect on learning and memory ability of D-galactose-induced dementia-mimicking mice (Li Shi Yong et al, Tianzhu powder is a study on the effect of improving learning and memory ability of D-galactose-induced dementia-mimicking mice, Shizhen national medicine [ J ], 2015, 09 th, 2056-2058). However, the therapeutic effect thereof is still to be improved.
Disclosure of Invention
The inventor of the application finds that the treatment effect of the obtained composition on cerebral ischemia is greatly improved after the proportion of the gastrodia elata and the trillium is changed and the borneol with proper proportion is added.
The invention aims to provide a traditional Chinese medicine composition which has a remarkable effect of treating cerebral ischemia.
The invention also aims to provide a preparation method of the traditional Chinese medicine composition.
The invention also aims to provide a preparation containing the traditional Chinese medicine composition.
The invention also aims to provide the application of the traditional Chinese medicine composition.
The purpose of the invention is realized by the following technical scheme.
The invention provides a traditional Chinese medicine composition for treating cerebral ischemia, which comprises the following active ingredients: 4-15 parts of gastrodia elata, 1-3 parts of trillium and 0.02-0.15 part of borneol.
According to the traditional Chinese medicine composition disclosed by the invention, preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 5-14 parts of gastrodia elata, 1.2-2.5 parts of trillium and 0.03-0.12 part of borneol.
According to the traditional Chinese medicine composition disclosed by the invention, preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 6-13 parts of gastrodia elata, 1.3-2.2 parts of trillium and 0.05-0.12 part of borneol.
According to the traditional Chinese medicine composition disclosed by the invention, preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines:
10-14 parts of gastrodia elata, 1.2-1.5 parts of trillium and 0.05-0.08 part of borneol; or
6-8 parts of gastrodia elata, 1.4-1.8 parts of trillium and 0.08-0.12 part of borneol; or
5-6 parts of gastrodia elata, 1.8-2.2 parts of trillium fortunei and 0.03-0.06 part of borneol.
According to the traditional Chinese medicine composition disclosed by the invention, more preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines:
12 parts of gastrodia elata, 1.31 parts of trillium and 0.061 parts of borneol; or
7.68 parts of gastrodia elata, 1.63 parts of trillium and 0.12 part of borneol; or
5 parts of gastrodia elata, 2.05 parts of trillium and 0.049 part of borneol.
The invention also provides a preparation method of the traditional Chinese medicine composition.
According to one embodiment of the present invention, the preparation method comprises the steps of: extracting the gastrodia elata and the trillium fortunei by adopting water as a solvent to obtain a water extract; and then uniformly mixing the water extract with borneol to obtain the traditional Chinese medicine composition.
According to another embodiment of the present invention, the preparation method comprises the steps of: extracting the gastrodia elata and the trillium fortunei by using an ethanol water solution with the concentration of 50-90 vol% as a solvent to obtain an ethanol extract; and then uniformly mixing the ethanol extract and borneol to obtain the traditional Chinese medicine composition.
The preparation method according to the present invention preferably comprises the steps of: heating and refluxing the gastrodia elata and the trillium fortunei for 1-4 times by using 50-90 vol% ethanol water solution as a solvent, wherein the dosage of the solvent is 6-12 times of the total weight of the gastrodia elata and the trillium fortunei each time, and the extraction time is 0.5-2 hours each time, so that an ethanol extract is obtained; and then uniformly mixing the ethanol extract and borneol to obtain the traditional Chinese medicine composition.
The invention also provides a traditional Chinese medicine preparation for treating cerebral ischemia, which comprises the traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials.
Furthermore, the invention also provides application of the traditional Chinese medicine composition in preparing a medicine for treating cerebral ischemia.
The invention adjusts the proportion of the gastrodia elata and the trillium fortunei and adds a proper amount of borneol to form the novel traditional Chinese medicine composition which has good treatment effect on cerebral ischemia. In the invention, after the dosage proportion of the gastrodia elata and the trillium is increased, the treatment effect on cerebral ischemia, particularly acute cerebral ischemia, is obviously improved. In addition, when the dosage of the borneol in the composition is controlled within the range of the invention, the treatment effect on cerebral ischemia is obviously enhanced. The effect is most pronounced according to a particularly preferred embodiment of the invention.
Detailed Description
The present invention will be further described with reference to the following specific examples, but the scope of the present invention is not limited thereto.
In the invention, the Gastrodia elata is a dried tuber of Gastrodia elata Bl. of Orchidaceae. In the present invention, the Trillium japonicum is rhizomes and fibrous roots of Trillium kamtschaticum pall.of Trillium genus of Trillium family, Trillium tschososkii Maxim., or Trillium govanicum wall.
< Chinese medicinal composition >
In the traditional Chinese medicine composition for treating cerebral ischemia, the effective components are prepared from gastrodia elata, trillium and borneol. The effective component refers to a component which plays a drug effect in the traditional Chinese medicine composition, and does not contain pharmaceutic adjuvant.
The effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 4-15 parts of gastrodia elata, 1-3 parts of trillium and 0.02-0.15 part of borneol. Preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 5-14 parts of gastrodia elata, 1.2-2.5 parts of trillium and 0.03-0.12 part of borneol. Preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 6-13 parts of gastrodia elata, 1.3-2.2 parts of trillium and 0.05-0.12 part of borneol.
According to one embodiment of the invention, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 10-14 parts of gastrodia elata, 1.2-1.5 parts of trillium and 0.05-0.08 part of borneol; or 6-8 parts of gastrodia elata, 1.4-1.8 parts of trillium and 0.08-0.12 part of borneol by weight; or 5 to 6 parts by weight of gastrodia elata, 1.8 to 2.2 parts by weight of trillium and 0.03 to 0.06 part by weight of borneol. According to another embodiment of the invention, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 10-14 parts of gastrodia elata, 1.2-1.5 parts of trillium and 0.05-0.08 part of borneol.
As a preferred embodiment of the invention, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 12 parts of gastrodia elata, 1.31 parts of trillium and 0.061 parts of borneol; or
7.68 parts of gastrodia elata, 1.63 parts of trillium and 0.12 part of borneol; or 5 parts of gastrodia elata, 2.05 parts of trillium and 0.049 part of borneol. More preferably, the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 12 parts of gastrodia elata, 1.31 parts of trillium and 0.061 parts of borneol. This may further improve the therapeutic effect on cerebral ischemia.
The effective components of the traditional Chinese medicine composition are only prepared from the gastrodia elata, the trillium fortunei and the borneol, and do not contain other raw material medicines or medicinal active components prepared from other raw material medicines.
The inventor finds that the application of the gastrodia elata and the trillium fortunei in the range of the invention has obvious treatment effect on cerebral ischemia. In addition, when the dosage of the borneol in the composition is controlled within the range of the invention, the treatment effect on cerebral ischemia is obviously enhanced; the dosage of the borneol is too small or too much, the synergistic effect is not obvious if the dosage is too small, and the brain is excessively damaged if the dosage is too much. According to a particularly preferred embodiment of the invention, the effect is most prominent when the mixture ratio of the three raw materials is 10-14 parts by weight of rhizoma gastrodiae, 1.2-1.5 parts by weight of trillium and 0.05-0.08 part by weight of borneol.
< preparation method >
The traditional Chinese medicine composition can be prepared by adopting various preparation methods.
According to one aspect of the invention, the preparation method of the traditional Chinese medicine composition comprises the following steps: extracting said gastrodia elata and trillium officinale with water as a solvent to obtain a water extract; and mixing the water extract with borneol to obtain the traditional Chinese medicine composition. The extraction method is selected from decocting, heating reflux extraction or ultrasonic extraction, and more preferably heating reflux extraction. According to one embodiment of the invention, the gastrodia elata and the trillium are respectively extracted by adding water, so that a gastrodia elata water extract and a trillium water extract are respectively obtained, and the gastrodia elata water extract and the trillium water extract are mixed to obtain the water extracts. According to another embodiment of the present invention, said water extract is obtained by mixing said gastrodia elata and said trillium, followed by extracting with water. The preparation method of the traditional Chinese medicine composition comprises the following steps: mixing the gastrodia elata and the trillium, adding water, and extracting for 1-4 times, preferably 2-3 times; the water adding amount is 5-15 times of the total weight of the gastrodia elata and the trillium, and preferably 6-8 times of the total weight of the gastrodia elata and the trillium; the extraction time is 0.5-3 hours, preferably 1-2 hours; concentrating the obtained extract, and drying to obtain the water extract; and then uniformly mixing the water extract with borneol to obtain the traditional Chinese medicine composition.
In the invention, the water extract is a broad concept, and can be water extract obtained by extracting gastrodia elata and trillium with water, water extract concentrated solution obtained by concentrating the water extract, and solid obtained by drying the water extract concentrated solution.
According to another aspect of the present invention, the preparation method of the Chinese medicinal composition comprises the following steps: extracting the gastrodia elata and the trillium fortunei by using an ethanol water solution with the concentration of 50-90 vol% as a solvent to obtain an ethanol extract; and mixing the ethanol extract with borneol to obtain the traditional Chinese medicine composition. The extraction method is selected from decocting, heating reflux extraction or ultrasonic extraction, and more preferably heating reflux extraction. Preferably, the concentration of the ethanol aqueous solution is 55-80 vol%, more preferably 65-75 vol%. According to one embodiment of the present invention, gastrodia elata and trillium are respectively added into ethanol aqueous solution as solvent for extraction, so as to respectively obtain gastrodia elata ethanol extract and trillium ethanol extract, and the gastrodia elata ethanol extract and the trillium ethanol extract are mixed to obtain the ethanol extracts. According to another embodiment of the present invention, said gastrodia elata and said trillium are mixed and then extracted with an aqueous ethanol solution to obtain said ethanol extract. In the present invention, the borneol may be directly mixed with the ethanol extract in a solid state; the borneol can also be dissolved in ethanol or ethanol water solution and then mixed with the ethanol extract, thereby ensuring the uniform mixing. The preparation method of the traditional Chinese medicine composition comprises the following steps: heating and refluxing the gastrodia elata and the trillium fortunei for 1-4 times by using 50-90 vol% ethanol water solution as a solvent, wherein the dosage of the solvent is 6-12 times of the total weight of the gastrodia elata and the trillium fortunei each time, and the extraction time is 0.5-2 hours each time, so that an ethanol extract is obtained; and mixing the ethanol extract with borneol to obtain the traditional Chinese medicine composition.
In the invention, the ethanol extract is a broad concept, and can be ethanol extract obtained by extracting gastrodia elata and trillium with ethanol water solution, ethanol extract concentrate obtained by concentrating the ethanol extract, or solid obtained by drying the ethanol extract concentrate.
The research on the extraction method shows that the content of the effective components in the obtained extract is higher than that of the single medicine by adopting water or ethanol water solution as the solvent for extraction and mixing and extracting the gastrodia elata and the trillium. Therefore, the compatibility of the traditional Chinese medicine composition promotes the dissolution of each raw material medicine, and is not the simple addition of single raw material medicines. The invention discovers that the traditional Chinese medicine composition prepared by the ethanol extraction method has more remarkable effect on treating cerebral ischemia.
< Chinese medicinal preparation and use >
The invention also provides a traditional Chinese medicine preparation for treating cerebral ischemia, and the traditional Chinese medicine preparation comprises the traditional Chinese medicine composition for treating cerebral ischemia and pharmaceutically acceptable auxiliary materials. As the pharmaceutically acceptable auxiliary materials, those known in the art, such as maltodextrin, starch, microcrystalline cellulose, magnesium stearate, etc., can be used, and will not be described herein. According to one embodiment of the present invention, the pharmaceutical preparation comprises only the Chinese medicinal composition as a pharmaceutically active ingredient, and does not comprise other pharmaceutically active ingredients. The dosage form of the preparation is not limited, and can be, for example, tablets, pills, capsules, granules, liquid preparations, injections, sprays, suppositories and the like.
The invention also provides application of the traditional Chinese medicine composition in preparing a medicine for treating cerebral ischemia. In the present invention, preferably, the cerebral ischemia is acute cerebral ischemia.
The embodiments and technical effects of the present invention will be described in detail below by way of specific examples and experimental examples.
Example 1
Taking 5 parts by weight of gastrodia elata, 2.05 parts by weight of trillium and 0.049 part by weight of borneol, firstly mixing the gastrodia elata and the trillium, adding 70 vol% ethanol water solution as a solvent, heating, refluxing and extracting for 2 times, wherein the amount of the solvent added in each time is 10 times of the total weight of the gastrodia elata and the trillium, the extraction time in each time is 45 minutes, and concentrating the obtained extracting solution to obtain a concentrated solution; dissolving borneol in a small amount of ethanol to obtain an ethanol solution of borneol, uniformly mixing the ethanol solution of borneol with the concentrated solution, and drying to obtain the traditional Chinese medicine composition A.
Example 2
Taking 7.68 parts by weight of gastrodia elata, 1.63 parts by weight of trillium and 0.12 part by weight of borneol, firstly mixing the gastrodia elata and the trillium, adding 70 vol% ethanol water solution as a solvent, heating, refluxing and extracting for 2 times, wherein the amount of the solvent added in each time is 10 times of the total weight of the gastrodia elata and the trillium, the extraction time in each time is 45 minutes, and concentrating the obtained extracting solution to obtain a concentrated solution; dissolving borneol in a small amount of ethanol to obtain an ethanol solution of borneol, uniformly mixing the ethanol solution of borneol with the concentrated solution, and drying to obtain the traditional Chinese medicine composition B.
Example 3
Taking 12 parts by weight of gastrodia elata, 1.31 parts by weight of trillium and 0.061 parts by weight of borneol, firstly mixing the gastrodia elata and the trillium, adding 70 vol% ethanol water solution as a solvent, heating, refluxing and extracting for 2 times, wherein the amount of the solvent added in each time is 10 times of the total weight of the gastrodia elata and the trillium, the extraction time in each time is 45 minutes, and concentrating the obtained extracting solution to obtain a concentrated solution; dissolving borneol in a small amount of ethanol to obtain an ethanol solution of borneol, uniformly mixing the ethanol solution of borneol with the concentrated solution, and drying to obtain the traditional Chinese medicine composition C.
Example 4
Taking 12 parts by weight of gastrodia elata, 1.31 parts by weight of trillium and 0.061 parts by weight of borneol, firstly mixing the gastrodia elata and the trillium, adding water as a solvent, heating, refluxing and extracting for 2 times, wherein the water amount is 8 times and 6 times of the total weight of the gastrodia elata and the trillium, the extraction time is 1 hour each time, and concentrating the obtained extracting solution to obtain a concentrated solution; dissolving borneol in a small amount of ethanol to obtain an ethanol solution of borneol, uniformly mixing the ethanol solution of borneol with the concentrated solution, and drying to obtain the traditional Chinese medicine composition D.
Example 5
Taking 6 parts by weight of gastrodia elata, 1.8 parts by weight of trillium and 0.036 part by weight of borneol, firstly mixing the gastrodia elata and the trillium, adding 65 vol% ethanol water solution as a solvent, heating, refluxing and extracting for 3 times, wherein the water amount is 10 times, 8 times and 8 times of the total weight of the gastrodia elata and the trillium each time, the extraction time is 1 hour, 0.75 hour and 0.5 hour respectively, concentrating the obtained extracting solution, and drying to obtain an ethanol extract; mixing Borneolum Syntheticum and the above extracts to obtain the Chinese medicinal composition E.
Example 6
Taking 10 parts by weight of gastrodia elata, 1.4 parts by weight of trillium and 0.05 part by weight of borneol, firstly mixing the gastrodia elata and the trillium, adding water as a solvent, heating, refluxing and extracting for 2 times, wherein the water amount added in each time is 8 times of the total weight of the gastrodia elata and the trillium, the extraction time in each time is 1.5 hours and 1 hour respectively, concentrating the obtained extracting solution, and drying to obtain a water extract; and mixing the water extract and borneol uniformly to obtain the traditional Chinese medicine composition F.
Example 7
The traditional Chinese medicine composition of the embodiment 1 is added with proper amount of maltodextrin and starch, and then the mixture is prepared into granules, dried, encapsulated and prepared into capsules.
Example 8
The Chinese medicinal composition of example 3 was added with compressible starch, microcrystalline cellulose and a small amount of magnesium stearate, and tableted to obtain tablets.
Comparative example 1
Mixing 12 parts by weight of rhizoma gastrodiae and 6 parts by weight of trillium, adding water as a solvent, heating, refluxing and extracting for 2 times, wherein the water amount is 8 times and 6 times of the total weight of the rhizoma gastrodiae and the trillium, the extraction time is 1 hour, and the obtained extract is concentrated and dried to obtain the traditional Chinese medicine composition D1.
The effect of the pharmaceutical composition of the present invention in treating cerebral ischemia is verified by the following pharmacological tests. The SD rats used in the following experiments were provided by the experimental animal technology ltd, viton, beijing, SPF grade. The butyl phthalide soft capsule (Enbip) is produced by Enbip pharmaceutical Co.
In the following experiments, the kato fractionation method used for fractionation statistics was performed with reference to the methods described in the following documents: kato H, Liu Y, Araki T, et al, temporal profile of the effects with branched ceramic influencing secondary influencing chemical interaction in the gel, cumulative impact and protective effects [ J ]. Brain Res,1991,553(2) 238-242.
1. Test method
1.1 modeling and grouping of laboratory animals
SD rats, male, with a body weight of 310 +/-10 g, were used to prepare a middle cerebral artery ischemia-reperfusion rat model by a wire-embolus method. In the molded rat, the symptom of neuro-behavioral abnormality is shown after the neuro-behavioral evaluation by a Longa method; and cerebral infarction was evaluated by TTC staining, and rats exhibiting non-infarcted areas and white-infarcted areas and a certain edema were successfully modeled (MCAO rats).
Randomly dividing MCAO rats into 7 groups, namely a model group, a butylphthalide administration group, a Chinese medicinal composition A, B, C, D administration group and a Chinese medicinal composition D1 administration group; a sham-operated group (healthy SD rats without molding) was also set as a blank control.
Wherein, the model group and the sham operation group are infused with distilled water every day. The butylphthalide group was administered daily (70 mg/kg); the traditional Chinese medicine composition A, the traditional Chinese medicine composition B and the traditional Chinese medicine composition C are respectively irrigated with stomach every day, the traditional Chinese medicine composition A (equivalent to 7.10g of crude drugs/kg), the traditional Chinese medicine composition B (equivalent to 9.43g of crude drugs/kg), the traditional Chinese medicine composition C (equivalent to 13.37g of crude drugs/kg), the traditional Chinese medicine composition D (equivalent to 13.37g of crude drugs/kg) and the traditional Chinese medicine composition D1 are irrigated with stomach every day, and the traditional Chinese medicine composition D1 (equivalent to 18g of crude drugs/kg) are respectively irrigated with stomach every.
The above drugs were administered continuously for 7 days, 1 time per day.
1.2 Experimental methods
After the administration is finished, the cerebral infarction volume of each group of rats is measured by adopting TTC staining, brain slices after the TTC staining are photographed by a digital camera, and the total volume of all brain slices and the total volume of an infarct area of each rat are respectively calculated by using a Count/Size module in a Measure column of Image-proplus 6.0 software to calculate the percent of the infarct volume.
And (3) carrying out paraffin embedding slicing on the brain slice after TTC staining, carrying out HE staining and Nissl staining on the section, then scanning the HE staining and Nissl staining section on the whole section by using a pathological section digital scanner, respectively selecting 3 visual fields in the ischemic side cortical functional area and the striatum, and counting the complete nerve cells in the 6 visual fields. According to HE staining and Nissl staining, histological change conditions of the cerebral ischemic lateral cortex area of the rat are observed, graded Statistics is carried out according to a kato grading method, and single-factor variance analysis Statistics is carried out on data by adopting IBM SPSS Statistics 20.0 software.
1.3 results of the experiment
1.3.1 TTC staining and cerebral infarction volume assessment
Compared with the model group, the rats of A, B, C, D group and butylphthalide group of the invention have obviously reduced cerebral infarction volume (P <0.05), wherein the C group of the invention is most obvious. The results are shown in Table 1.
TABLE 1 cerebral infarct volume in MCAO rats
| Grouping | N | Cerebral infarct volume |
| Artificial operation group | 6 | 0 |
| Model set | 6 | 35.67±8.96 |
| Butylphthalide group | 6 | 12.67±9.27* |
| group-A of the present invention | 6 | 15.50±13.95* |
| Group B of the present invention | 6 | 6.83±7.52*# |
| Group C of the present invention | 6 | 2.00±2.68*# |
| Group D of the present invention | 6 | 17.15±19.01 |
| Comparative examples 1 to D1 groups | 6 | 26.00±21.01 |
Note: denotes P <0.05 compared to model group and # denotes P <0.05 compared to D1.
1.3.2 HE staining and Nissl staining results
HE staining and Nissl staining show that the brain slice structure of the rat in the sham operation group is complete and clear, the arrangement of nerve cells is compact, the number of nerve cells is large, and the cell structure is complete; the tissue structure of the rat brain tissue infarct area of the model group is loose, so that complete nerve cells are difficult to find, and a certain edema phenomenon exists; compared with the model group, the A, B, C, D group and the butylphthalide group of the invention have obviously increased number of intact cells (P <0.05), and the edema condition is also lighter.
TABLE 2 number of cerebral ischemic side nerve cells in MCAO rats
Note: denotes P <0.05, N6 compared to model group.
1.3.3 fractional statistics
Grading statistics are carried out on histological changes of the cerebral ischemic side cortical areas of the rats after HE staining and Nissl staining by adopting a kato grading method. Statistics shows that the cerebral ischemic lateral cortex of the rats in the model group can see death of the neurones in pieces, and the infarct size is greater than 1/3 of the total area of the ischemic lateral cortex; the rats in the butylphthalide group, group B and group C of the present invention had less brain neuron death and significantly reduced grade scores (P <0.05) compared to the model group. The results are shown in Table 3.
TABLE 3 pathological morphological changes of the cerebral cortex of MCAO rats
| Group of | Class I | Stage II | Class III | IV stage |
| Group of sham operations | 0 | 0 | 0 | 0 |
| Model set | 0 | 0 | 3 | 3 |
| Butylphthalide group | 2 | 2 | 1 | 1 |
| group-A of the present invention | 1 | 2 | 1 | 2 |
| Group B of the invention | 2 | 3 | 1 | 0 |
| Group C of the invention | 3 | 2 | 1 | 0 |
| Comparative examples 1 to D1 groups | 1 | 1 | 3 | 1 |
Note: denotes P <0.05, N6 compared to model group.
The present invention is not limited to the above-described embodiments, and any variations, modifications, and substitutions which may occur to those skilled in the art may be made without departing from the spirit of the invention.
Claims (8)
1. A traditional Chinese medicine composition for treating cerebral ischemia is characterized in that the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines: 6-13 parts of gastrodia elata, 1.3-2.2 parts of trillium and 0.05-0.12 part of borneol.
2. A traditional Chinese medicine composition for treating cerebral ischemia is characterized in that the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines:
10-14 parts of gastrodia elata, 1.2-1.5 parts of trillium and 0.05-0.08 part of borneol; or
6-8 parts of gastrodia elata, 1.4-1.8 parts of trillium and 0.08-0.12 part of borneol; or
5-6 parts of gastrodia elata, 1.8-2.2 parts of trillium fortunei and 0.03-0.06 part of borneol.
3. The traditional Chinese medicine composition according to claim 2, wherein the effective components of the traditional Chinese medicine composition are prepared from the following raw material medicines:
12 parts of gastrodia elata, 1.31 parts of trillium and 0.061 parts of borneol; or
7.68 parts of gastrodia elata, 1.63 parts of trillium and 0.12 part of borneol; or
5 parts of gastrodia elata, 2.05 parts of trillium and 0.049 part of borneol.
4. A method for preparing a Chinese medicinal composition according to any one of claims 1 to 3, which comprises the following steps: extracting the gastrodia elata and the trillium fortunei by adopting water as a solvent to obtain a water extract; and then uniformly mixing the water extract with borneol to obtain the traditional Chinese medicine composition.
5. A method for preparing a Chinese medicinal composition according to any one of claims 1 to 3, which comprises the following steps: extracting the gastrodia elata and the trillium fortunei by using an ethanol water solution with the concentration of 50-90 vol% as a solvent to obtain an ethanol extract; and then uniformly mixing the ethanol extract and borneol to obtain the traditional Chinese medicine composition.
6. A method for preparing a Chinese medicinal composition according to any one of claims 1 to 3, which comprises the following steps: heating and refluxing the gastrodia elata and the trillium fortunei for 1-4 times by using 50-90 vol% ethanol water solution as a solvent, wherein the dosage of the solvent is 6-12 times of the total weight of the gastrodia elata and the trillium fortunei each time, and the extraction time is 0.5-2 hours each time, so that an ethanol extract is obtained; and mixing the ethanol extract with borneol to obtain the traditional Chinese medicine composition.
7. A traditional Chinese medicine preparation for treating cerebral ischemia, which consists of the traditional Chinese medicine composition according to any one of claims 1 to 3 and pharmaceutically acceptable auxiliary materials.
8. Use of the traditional Chinese medicine composition according to any one of claims 1 to 3 for preparing a medicament for treating cerebral ischemia.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710652742.6A CN107362287B (en) | 2017-08-02 | 2017-08-02 | Traditional Chinese medicine composition for treating cerebral ischemia and preparation method and application thereof |
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| CN201710652742.6A CN107362287B (en) | 2017-08-02 | 2017-08-02 | Traditional Chinese medicine composition for treating cerebral ischemia and preparation method and application thereof |
Publications (2)
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| CN107362287A CN107362287A (en) | 2017-11-21 |
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| 冰片注射液的抗脑缺血作用;何晓静等;《山西医药杂志》;20070930;第36卷(第9期);第795页左栏第1段，第795页第3节 * |
| 土家药天珠散对脑缺血所致大鼠学习记忆障碍的改善作用;宋阔魁等;《中草药》;20150228;第46卷(第3期);第394页左栏倒数第1段-右栏、第395页左栏第1-2段 * |
| 土家药延龄草抗SH_SY5Y细胞谷氨酸损伤有效部位筛选研究;李志勇;《中华中医药杂志》;20140731;第29卷(第7期);第2125-2129页 * |
| 基于系统药理学的天珠散抗血管性痴呆机制研究;张百霞等;《中华中医药杂志》;20150531;第30卷(第5期);第1425-1431页 * |
| 天珠散对D-半乳糖拟痴呆小鼠的治疗作用;毕天等;《时珍国医国药》;20131231;第24卷(第11期);第2593-2595页 * |
| 天珠散抗血管性痴呆的药效物质基础研究;毕天;《万方数据》;20141128;第3页第1-2段、第5页倒数第2段倒数第1-3行 * |
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