CN107348252A - Striped rice borer feed and its preparation and application - Google Patents
Striped rice borer feed and its preparation and application Download PDFInfo
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- CN107348252A CN107348252A CN201610302851.0A CN201610302851A CN107348252A CN 107348252 A CN107348252 A CN 107348252A CN 201610302851 A CN201610302851 A CN 201610302851A CN 107348252 A CN107348252 A CN 107348252A
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- Prior art keywords
- feed
- rice borer
- striped rice
- larvae
- carrot
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- 241000426497 Chilo suppressalis Species 0.000 title claims abstract description 98
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 29
- 244000000626 Daucus carota Species 0.000 claims abstract description 27
- 235000002767 Daucus carota Nutrition 0.000 claims abstract description 27
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 38
- 238000010010 raising Methods 0.000 claims description 31
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 30
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 20
- 241000209094 Oryza Species 0.000 claims description 19
- 235000007164 Oryza sativa Nutrition 0.000 claims description 19
- 235000009566 rice Nutrition 0.000 claims description 19
- 229960000583 acetic acid Drugs 0.000 claims description 17
- 230000007613 environmental effect Effects 0.000 claims description 17
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- 241000426499 Chilo Species 0.000 claims description 16
- 102000002322 Egg Proteins Human genes 0.000 claims description 16
- 108010000912 Egg Proteins Proteins 0.000 claims description 16
- 241000382353 Pupa Species 0.000 claims description 16
- 210000004681 ovum Anatomy 0.000 claims description 16
- 235000012054 meals Nutrition 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 229930006000 Sucrose Natural products 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 239000005018 casein Substances 0.000 claims description 13
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 13
- 235000021240 caseins Nutrition 0.000 claims description 13
- 239000012362 glacial acetic acid Substances 0.000 claims description 13
- 239000005720 sucrose Substances 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 10
- 235000019743 Choline chloride Nutrition 0.000 claims description 10
- 229930003270 Vitamin B Natural products 0.000 claims description 10
- 235000012000 cholesterol Nutrition 0.000 claims description 10
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 10
- 229960003178 choline chloride Drugs 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 10
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 10
- 235000010199 sorbic acid Nutrition 0.000 claims description 10
- 239000004334 sorbic acid Substances 0.000 claims description 10
- 229940075582 sorbic acid Drugs 0.000 claims description 10
- 235000019156 vitamin B Nutrition 0.000 claims description 10
- 239000011720 vitamin B Substances 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 235000019256 formaldehyde Nutrition 0.000 claims description 9
- 235000010323 ascorbic acid Nutrition 0.000 claims description 8
- 229960005070 ascorbic acid Drugs 0.000 claims description 8
- 239000011668 ascorbic acid Substances 0.000 claims description 8
- 230000032669 eclosion Effects 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 7
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 7
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 7
- 235000011054 acetic acid Nutrition 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 229940107161 cholesterol Drugs 0.000 claims description 5
- 230000019617 pupation Effects 0.000 claims description 5
- 239000004459 forage Substances 0.000 claims description 3
- 210000004894 snout Anatomy 0.000 claims description 3
- 238000007711 solidification Methods 0.000 claims description 3
- 230000008023 solidification Effects 0.000 claims description 3
- 229940052810 complex b Drugs 0.000 claims description 2
- 229940023476 agar Drugs 0.000 claims 1
- 235000010419 agar Nutrition 0.000 claims 1
- 241000746966 Zizania Species 0.000 description 12
- 235000002636 Zizania aquatica Nutrition 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 241000238631 Hexapoda Species 0.000 description 7
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 4
- 238000009835 boiling Methods 0.000 description 3
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- 230000003020 moisturizing effect Effects 0.000 description 3
- 230000017448 oviposition Effects 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- GDSOZVZXVXTJMI-SNAWJCMRSA-N (e)-1-methylbut-1-ene-1,2,4-tricarboxylic acid Chemical compound OC(=O)C(/C)=C(C(O)=O)\CCC(O)=O GDSOZVZXVXTJMI-SNAWJCMRSA-N 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 2
- 150000001243 acetic acids Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 2
- 229960002079 calcium pantothenate Drugs 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 229960000304 folic acid Drugs 0.000 description 2
- 235000019152 folic acid Nutrition 0.000 description 2
- 239000011724 folic acid Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000003760 hair shine Effects 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical class COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229960003966 nicotinamide Drugs 0.000 description 2
- 235000005152 nicotinamide Nutrition 0.000 description 2
- 239000011570 nicotinamide Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 229960002477 riboflavin Drugs 0.000 description 2
- 235000019192 riboflavin Nutrition 0.000 description 2
- 239000002151 riboflavin Substances 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 229960000344 thiamine hydrochloride Drugs 0.000 description 2
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 2
- 239000011747 thiamine hydrochloride Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 description 1
- 241001237160 Kallima inachus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
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- 230000009261 transgenic effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
This application provides the striped rice borer feed comprising carrot prepared product.The method of striped rice borer is raised present invention also provides the preparation method of the feed and using it.
Description
Technical field
The present invention relates generally to insect raising field.More specifically, the application is related to striped rice borer
Feed, feed producing method and the method using its raising striped rice borer.
Background technology
Striped rice borer (Chilo suppressalis (Walker)), popular name borer, borer, moth stalk worm etc.,
Paddy growth is endangered very big.Chilo spp larvae moth food stalk, causes the withered heart.Striped rice borer, which has, to be turned
Strain harmness characteristics, a Chilo spp larvae can endanger more plants of rice, cause dead leaf sheath, the withered heart.
At present, the research of striped rice borer comprehensive preventive health measures concentrates on novel biopesticide and transgenosis resists
In terms of the research and development of worm rice, and the screening of biological pesticide, measure, resistance mechanism and transgenic pest-resistant
The research and development of rice are required to striped rice borer worm sources large batch of, that development is consistent.
Striped rice borer artificial feeding mainly uses natural feed (for example, rice seedlings, wild rice stem) or semi-artificial feeding
Material is raised.Since the 1970s, researcher, which develops, utilizes natural feed or half people
Method for breeding of the work feed to striped rice borer.In addition, also have to the man-made feeds of striped rice borer in this area
Report, such as Han Lanzhi et al. disclose two in No. 200910080336.2 Chinese patent application
Change snout moth's larva man-made feeds.
This area, which still needs, develops new chilo suppressalis artificial feed.
Summary of the invention
In a first aspect, this application provides striped rice borer feed, it includes carrot prepared product.
In some embodiments, striped rice borer feed includes glacial acetic acid.
In some embodiments, striped rice borer feed include soy meal, dusty yeast, casein,
Any combination of sucrose or more.
In some embodiments, the weight based on striped rice borer feed, feed are included with raw material recklessly
The weight meter of radish, 7.5-22.5% carrot prepared product.
In some embodiments, the weight based on striped rice borer feed, feed include 0.1-1%'s
Glacial acetic acid.
In some embodiments, the weight based on striped rice borer feed, feed include 4.5-5.5%
Soy meal.
In some embodiments, the weight based on striped rice borer feed, feed include 3-4% ferment
Female powder.
In some embodiments, the weight based on striped rice borer feed, feed include 1.5-2%'s
Casein.
In some embodiments, the weight based on striped rice borer feed, feed include 1.5-2%'s
Sucrose.
In some embodiments, striped rice borer feed includes and is selected from following component：Agar, resist
Bad hematic acid, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound, sorbic acid, to hydroxyl
Any combination of yl benzoic acid methyl esters, formaldehyde and the above.
Second aspect, this application provides the preparation method of striped rice borer feed, comprise the following steps：
(1) homogenate is made in carrot and obtains carrot prepared product, add soy meal, dusty yeast,
Casein and sucrose, after stirring, autoclaving is carried out, obtains component A；
(2) water is added into agar and is heated to dissolving, is mixed with component A, after stirring
Cooling, obtains component B；
(3) by ascorbic acid, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound,
Sorbic acid and methyl p-hydroxybenzoate are dissolved in water, are mixed with component B, then with formaldehyde and ice
Acetic acid solution mixes.
In some embodiments, preparation method also includes making the product cooling obtained from step (3)
The step of solidification.
The third aspect, this application provides striped rice borer feed prepared by the method described in second aspect.
Fourth aspect, this application provides the method for raising striped rice borer, including with first aspect or
Striped rice borer forage feed striped rice borer described in the third aspect.
In some embodiments, method for breeding includes following one or more steps：
(1) newly hatched larvae is to pupating：The striped rice borer feed is placed in raising box, will just be incubated
Larva is placed on feed, and raising box is placed in incubator, was added or was changed every 2-5 days and raises
Material, until larvae pupation, the environmental condition in the incubator is 28 ± 1 DEG C of temperature, relatively
Humidity 70-80%, light application time 16L:8D；
(2) pupate to emergence：When Chilo spp larvae was developed to for 6 age, in other raising box
It is middle to add the feed and allow Chilo spp larvae to pupate, pupa is chosen after pupating, distinguished female
After hero, it is respectively put into culture dish, is placed in step (1) and is raised under same environmental conditions, until
Adult eclosion；
(3) sprout wings to spawning：Adult after emergence is put into transplanting the production of tillering stage rice seedlings
Mate and oviposit is carried out in ovum cage；
(4) ovum is to newly hatched larvae：The rice leaf that production has striped rice borer ovum is removed, is put into culture dish,
It is subsequently placed in step (1) and is developed under same environmental conditions, until larvae hatch.
Brief Description Of Drawings
Fig. 1 shows that the Chilo spp larvae Contrast on effect of two kinds of feed raisings in testing example shines
Piece, Figure 1A show the Chilo spp larvae (scale 1mm) that the feed prepared with embodiment 1 is raised；
Figure 1B is shown with the Chilo spp larvae (scale 1mm) raised based on wild rice stem feed.
Fig. 2 shows the striped rice borer pupa Contrast on effect photo of two kinds of feed raisings in testing example,
Fig. 2A shows the striped rice borer pupa (scale 1mm) that the feed prepared with embodiment 1 is raised；
Fig. 2 B are shown with the striped rice borer pupa (scale 1mm) raised based on wild rice stem feed.According to vision
Evaluate, the striped rice borer pupa in Fig. 2A is more than Fig. 2 B striped rice borer pupa.
Fig. 3 shows the rice stem borer contrast photo of two kinds of feed raisings in testing example,
Fig. 3 A show the rice stem borer that the feed prepared with embodiment 1 is raised, and Fig. 3 B are shown
With the rice stem borer (wherein finger-type teat glass d=20mm) raised based on wild rice stem feed.
Fig. 4 shows that the striped rice borer pieces of an egg Contrast on effect of two kinds of feed raisings in testing example shines
Piece, Fig. 4 A show the striped rice borer pieces of an egg that the feed prepared with embodiment 1 is raised, and Fig. 4 B show
Show with the striped rice borer pieces of an egg (scale 1mm) raised based on wild rice stem feed.According to visual evaluation,
Striped rice borer worm monolithic ovum grain number in Fig. 4 A is more than in Fig. 4 B (scale 1mm).
Fig. 5 shows the photo of the exemplary insect box device of the method for breeding for the application
(raising box specification is d=75mm, h=10mm, and lid specification is d=85mm, h=42mm,
Copper mesh specification above lid is d=60mm).
Detailed description of the Invention
Current striped rice borer is natural or man-made feeds are all based on plant biolobic material substantially.This Shen
Inventor please is realized with innovating formula from carrot as striped rice borer feed primary raw material
Preferable technique effect.In addition, present inventor, which also found using glacial acetic acid, is used as feed
Preferable antiseptic effect can be realized during preservative.Therefore, the application provide striped rice borer feed,
Feed producing method or successive Mass Rearing of The Rice Stem Borer Chilo Suppressalis Walker can provide following one or more advantages：
1. Chilo spp larvae is good to feed preference, grow good, pupa weight, 15 days
Larva average viability, 15 days Weight of larvae, averagely go through the phase, percentage of pupation, eclosion rate, sex ration,
Effect is good in terms of one or more biological indicators in single female egg laying amount, egg hatching rate.
2. the carrot as primary raw material is all readily available in full season, cheap, fresh-keeping
Degree is high, can reduce feed manufacturing cost, and ensure feed quality.
3. preferable antiseptic effect can be realized when adding glacial acetic acid as feed anticorrosion agent, one
The feed shelf-life of more than 2 months can be provided in a little examples.Under normal circumstances, not ice acetic acid
Can be preserved at 4 DEG C 45 days, add glacial acetic acid feed saved 75 days at 4 DEG C after insect still
Can normal feeding.
4. the feed and method of the application are raised suitable for extensive striped rice borer.
In a first aspect, this application provides striped rice borer feed, it includes carrot prepared product.
Term " carrot prepared product " used herein refers to what is obtained by carrot by processing
Suitable for manufacturing any kind of material based on carrot of striped rice borer feed, including, but it is unlimited
In, the water-based homogenate of carrot, mashed carrot, carrot meal mince, carrot block etc..
In some embodiments, striped rice borer feed includes the glacial acetic acid as preservative.Ability
Field technique personnel are it is understood that the glacial acetic acid that the glacial acetic acid that can be used includes any concentration is prepared
Product, such as 50% glacial acetic acid.
In some embodiments, striped rice borer feed include soy meal, dusty yeast, casein,
Any combination of sucrose or more.Soy meal, dusty yeast, casein, sucrose are insect feedstuffs
In the component commonly used, those skilled in the art can be readily determined and obtain this of suitable form
A little components.
In some embodiments, the weight based on striped rice borer feed, feed are included with raw material recklessly
The weight meter of radish, 7.5-22.5% carrot prepared product.In some embodiments, it is based on
The weight of striped rice borer feed, feed include 0.1-1% glacial acetic acid.In some embodiments,
Based on the weight of striped rice borer feed, feed includes 4.5-5.5% soy meal.In some embodiment party
In case, based on the weight of striped rice borer feed, feed includes 3-4% dusty yeast.In some implementations
In scheme, based on the weight of striped rice borer feed, feed includes 1.5-2% casein.At some
In embodiment, based on the weight of striped rice borer feed, feed includes 1.5-2% sucrose.
In some embodiments, striped rice borer feed includes and is selected from following component：Agar, resist
Bad hematic acid, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound, sorbic acid, to hydroxyl
Any combination of yl benzoic acid methyl esters, formaldehyde and the above.Said components are also in insect feedstuff
Conventional component, those skilled in the art can be readily determined and obtain these of suitable form
Component.In some embodiments, the weight based on striped rice borer feed, feed include 0.1-1%
Ascorbic acid.In some embodiments, the weight based on striped rice borer feed, feed include
0.01-0.1% cholesterol.In some embodiments, the weight based on striped rice borer feed, raise
Material includes 0.01-0.1% Choline Chloride.In some embodiments, based on striped rice borer feed
Weight, feed include 0.01-0.1% Wei Shi salt.In some embodiments, based on striped rice borer
The weight of feed, feed include 0.001-0.01% vitamin B compound.In some embodiments
In, based on the weight of striped rice borer feed, feed includes 0.1-1% sorbic acid.In some implementations
In scheme, based on the weight of striped rice borer feed, feed includes 0.1-1% P-hydroxybenzoic acid first
Ester.In some embodiments, the weight based on striped rice borer feed, feed include 1-5% fine jade
Fat.In some embodiments, the weight based on striped rice borer feed, feed include 0.05-0.5%
Formaldehyde.
Second aspect, this application provides the preparation method of striped rice borer feed, comprise the following steps：
(1) homogenate is made in carrot and obtains carrot prepared product, add soy meal, dusty yeast,
Casein and sucrose, after stirring, autoclaving is carried out, obtains component A；
(2) water is added into agar and is heated to dissolving, is mixed with component A, after stirring
Cooling, obtains component B；
(3) by ascorbic acid, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound,
Sorbic acid and methyl p-hydroxybenzoate are dissolved in water, are mixed with component B, then with formaldehyde and ice
Acetic acid solution mixes.
In some embodiments, preparation method also includes making the product cooling obtained from step (3)
The step of solidification.
As the example of nonrestrictive preparation method, can implement according to following steps：
A, carrot is cut into block, is proportionally added into water, be put into agitator and grind and be homogenized, it is even
It is poured into the container for filling soy meal, dusty yeast, casein and sucrose, is sufficiently stirred after slurry
After uniformly, it is placed in portable high-pressure steam sterilizing pan, sterilize 15min at 125 DEG C, has sterilized
Bi Hou, take out component A to be used as；
B, pure water is added in agar, heats and boils to boiling, until completely dissolved, be poured into
In component A, stir, and be cooled to 60 DEG C or so standby conduct component B；
C, in proportion by ascorbic acid, cholesterol, Choline Chloride, Wei Shi salt, B B-complex
B, sorbic acid and methyl p-hydroxybenzoate are put into beaker, are dissolved with a small amount of warm water, then will
It is poured into component B, then is proportionally added into the formaldehyde of concentration 40% and the glacial acetic acid of concentration 50%,
Stirring is until substantially uniformity；
D, it after feed is stirred, need to pour into crisper, 4 DEG C are deposited in after complete cooled and solidified
It is cleavable into fritter when to be used in refrigerator.
It should be appreciated that in the case where not conflicting, embodiment described in first aspect and
Technical characteristic is also applied for second aspect.
The third aspect, this application provides striped rice borer feed prepared by the method described in second aspect.
Fourth aspect, this application provides the method for raising striped rice borer, including with first aspect or
Striped rice borer forage feed striped rice borer described in the third aspect.
In some embodiments, method for breeding includes following one or more steps：
(1) newly hatched larvae is to pupating：The striped rice borer feed is placed in raising box, will just be incubated
Larva is placed on feed, and raising box is placed in incubator, was added or was changed every 2-5 days and raises
Material, until larvae pupation, the environmental condition in the incubator is 28 ± 1 DEG C of temperature, relatively
Humidity 70-80%, light application time 16L:8D；
(2) pupate to emergence：When Chilo spp larvae was developed to for 6 age, in other raising box
It is middle to add the feed and allow Chilo spp larvae to pupate, pupa is chosen after pupating, distinguished female
After hero, it is respectively put into culture dish, is placed in step (1) and is raised under same environmental conditions, until
Adult eclosion；
(3) sprout wings to spawning：Adult after emergence is put into transplanting the production of tillering stage rice seedlings
Mate and oviposit is carried out in ovum cage；
(4) ovum is to newly hatched larvae：The rice leaf that production has striped rice borer ovum is removed, is put into culture dish,
It is subsequently placed in step (1) and is developed under same environmental conditions, until larvae hatch.
As the example of nonrestrictive preparation method, can implement according to following steps：
A, newly hatched larvae is to pupating：Feed is cut into cuboid and is placed on raising cassette bottom portion, will just incubate children
Worm is placed on feed, and covers the lid of copper mesh, is placed in plant incubator and is raised, every 5
1 feed is changed in its addition, until pupating, the environmental condition in plant incubator is temperature
28 ± 1 DEG C, relative humidity 70-80%, light application time 16L:8D；
B, pupate to emergence：When Chilo spp larvae was developed to for 6 age, add in new raising cassette bottom portion
One piece of feed is simultaneously pupated in feed upper ends blotting paper creasy for it, chooses pupa after pupating
Go out, after distinguishing male and female, be respectively put into batch cultur ware, be placed in identical environment bar in step A
Raised under part, until adult eclosion；
C, sprout wings to spawning：Adult after emergence is put into transplanting the spawning cage of tillering stage rice seedlings
Middle carry out mate and oviposit, and raise and supplemented the nutrients with 10% hydromel, the spawning of adult is checked daily
Situation simultaneously changes fresh rice seedlings；
D, ovum is to newly hatched larvae：The rice leaf that production has striped rice borer ovum is removed, is put into and is covered with wet filter paper
In batch cultur ware, and in one layer of wet filter paper moisturizing of upper cover, it is subsequently placed in identical in step A
Developed under environmental condition, until larvae hatch.
It is it should be appreciated that discussed in detail above only for making those skilled in the art more clearly understand this
The content of application, and be not intended to be any limitation as in any way.Those skilled in the art can be right
The embodiment carries out various changes and change.
Embodiment
Following examples are provided and further describe the application, and are not limited.
Prepare embodiment 1：
The formula for preparing 2000g feeds is as follows：
Vitamin B compound formula is as follows：
Niacinamide：0.0060g；Thiamine hydrochloride (VB1)：0.0016g；Riboflavin (VB2)：0.0030g；
Puridoxine hydrochloride (VB6)：0.0016g；Rely ammonia inositol (VB12)：0.00004g；Folic acid:0.0016
g；Calcium pantothenate：0.0060g；Biotin：0.00016g；Amount to：0.020g.
Feed formulation method：
1st, each component is weighed by proportioning, it is standby；
2nd, carrot is cut into block, and adds 700ml water, be put into agitator and grind and be homogenized,
It is poured into after homogenate in the container for filling soy meal, dusty yeast, casein and sucrose, fully stirs
After mixing uniformly, it is placed in portable high-pressure steam sterilizing pan, sterilize 15min at 125 DEG C, sterilizing
After, take out component A to be used as；
3rd, 725.28ml pure water is added in agar, heats and boils to boiling, until completely dissolved,
It is poured into component A, firmly stirs, and is cooled to 60 DEG C or so and standby is used as B component；
4th, by the ascorbic acid weighed up, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound,
Sorbic acid and methyl p-hydroxybenzoate are put into beaker, are dissolved with 120ml warm waters, then will
It is poured into B, adds the formaldehyde of 1.8ml 40% and the glacial acetic acids of 40ml 50%, and stirring is until completely
Uniformly；
5th, after feed is stirred, pour into crisper, 4 DEG C are deposited in after complete cooled and solidified
In refrigerator.
Prepare embodiment 2：
It is as follows to prepare 2000g feed formulas：
Attached vitamin B compound formula：
Niacinamide：0.0060g；Thiamine hydrochloride (VB1)：0.0016g；Riboflavin (VB2)：
0.0030g；Puridoxine hydrochloride (VB6)：0.0016g；Rely ammonia inositol (VB12)：0.00004g；
Folic acid:0.0016g；Calcium pantothenate：0.0060g；Biotin：0.00016g；Amount to：0.020g.
Feed formulation method：
1st, each component is weighed by proportioning, it is standby；
2nd, carrot is cut into block, and adds 700ml water, be put into agitator and grind and be homogenized,
It is poured into after homogenate in the container for filling soy meal, dusty yeast, casein and sucrose, fully stirs
After mixing uniformly, it is placed in portable high-pressure steam sterilizing pan, sterilize 15min at 125 DEG C, sterilizing
After, take out component A to be used as；
3rd, 480.28ml pure water is added in agar, heats and boils to boiling, until completely dissolved,
It is poured into component A, firmly stirs, and is cooled to 60 DEG C or so and standby is used as B component；
4th, by the ascorbic acid weighed up, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound,
Sorbic acid and methyl p-hydroxybenzoate are put into beaker, are dissolved with 100ml warm waters, then will
It is poured into B, adds the formaldehyde of 1.8ml 40% and the glacial acetic acids of 5ml 50%, and stirring is until completely
It is even；
5th, after feed is stirred, pour into crisper, 4 DEG C are deposited in after complete cooled and solidified
In refrigerator.
Testing example
The present embodiment is the effect in order to test the striped rice borer feed of the application in striped rice borer is raised.
As a comparison, the feed based on apple with being prepared according to the method for embodiment 1 is also tested in the lump
(hereinafter referred " apple feed ", wherein the carrot raw material in embodiment 1 is replaced with into apple, its
Remaining technique is identical) and according in No. 200910080336.2 Chinese patent application method prepare base
In the feed (hereinafter referred " wild rice stem feed ") of wild rice stem.
Materials and methods：
For trying feed：
Feed, apple feed and wild rice stem feed prepared by testing example l.
The method for breeding of striped rice borer：
1st, newly hatched larvae is to pupating：Man-made feeds are cut into thick 0.2mm, long 30mm, wide 15mm
Cuboid is placed on raising box (d=7.5cm, h=10cm) bottom, and newly hatched larvae is placed on feed,
And copper mesh (d=6cm) lid (d=8.5cm, h=4.2cm) is covered, it is placed in plant incubator
Interior raising, added every 5 days or change 1 feed, until pupating, in the plant incubator
Environmental condition be 28 ± 1 DEG C, relative humidity 70-80%, light application time 16L of temperature:8D；
2nd, pupate to emergence：When Chilo spp larvae was developed to for 6 age, add in new raising cassette bottom portion
One piece of feed is simultaneously pupated in feed upper ends blotting paper creasy for it, chooses pupa after pupating
Go out, after distinguishing male and female, be respectively put into batch cultur ware (d1=8.5cm, d2=9cm), be placed in
Raised in step 1 under same environmental conditions, until adult eclosion；
3rd, sprout wings to spawning：Adult after emergence is put into transplanting the spawning cage of tillering stage rice seedlings
Mate and oviposit is carried out in (a=40cm, b=40cm, c=80cm), and raises and is supplemented with 10% hydromel
Nutrition, the spawning situation of adult is checked daily and changes fresh rice seedlings；
4th, ovum is to newly hatched larvae：The rice leaf that production has striped rice borer ovum is removed, is put into and is covered with wet filter paper
In batch cultur ware, and in one layer of wet filter paper moisturizing of upper cover, it is subsequently placed in identical in step 1
Developed under environmental condition, until larvae hatch.
5th, after larvae hatch, continue by the circulate operation continuously rearing of above-mentioned steps 1,2,3,4,
Worm sources needed for constantly providing, until raising terminates.
Selected insect source：
From Hubei Xiaogan experimental field gather Chilo spp larvae, with wild rice stem feed raise to pupate, adult
Sprout wings, after spawning, it is selected insect source to remove generation newly hatched larvae.
The method for breeding of striped rice borer：
1st, newly hatched larvae is to pupating：Man-made feeds are cut into thick 0.2mm, long 30mm, wide 15mm
Cuboid is placed on raising box (d=7.5cm, h=10cm, as shown in Figure 5) bottom, by newly hatched larvae
It is placed on feed, and covers copper mesh (d=6cm) lid (d=8.5cm, h=4.2cm), puts
In being raised in plant incubator, added every 5 days or change 1 feed, until pupate, it is described
Environmental condition in plant incubator is 28 ± 1 DEG C, relative humidity 70-80% of temperature, light application time
16L:8D；
2nd, pupate to emergence：When Chilo spp larvae was developed to for 6 age, add in new raising cassette bottom portion
One piece of feed is simultaneously pupated in feed upper ends blotting paper creasy for it, chooses pupa after pupating
Go out, after distinguishing male and female, be respectively put into batch cultur ware (d1=8.5cm, d2=9cm), be placed in
Raised in step 1 under same environmental conditions, until adult eclosion；
3rd, sprout wings to spawning：Adult after emergence is put into transplanting the spawning cage of tillering stage rice seedlings
Mate and oviposit is carried out in (a=40cm, b=40cm, c=80cm), and raises and is supplemented with 10% hydromel
Nutrition, the spawning situation of adult is checked daily and changes fresh rice seedlings；
4th, ovum is to newly hatched larvae：The rice leaf that production has striped rice borer ovum is removed, is put into and is covered with wet filter paper
In batch cultur ware, and in one layer of wet filter paper moisturizing of upper cover, it is subsequently placed in identical in step 1
Developed under environmental condition, until larvae hatch.
5th, after larvae hatch, continue by the circulate operation continuously rearing of above-mentioned steps 1,2,3,4,
Worm sources needed for constantly providing, until raising terminates.
Evaluation method：
If 3 feed prepared by embodiment l, apple feed and wild rice stem feed treatment groups, each experiment
1 generation of continuous raising, for trying larva 200, recorded in feeding process larva development duration,
Survival rate, percentage of pupation, eclosion rate, female pupa weight, male pupa weight, sex ration, egg laying amount and egg hatching
Rate.Each experiment is repeated 3 times altogether.This is tested exists in by the end of October, 2015 in the first arrival of in August, 2015
Life Sci-Tech central laboratory of Co., Ltd of Chinese subset group completes.
Data statistics：
Initial analysis is carried out to data using Excel, the data obtained entered using SAS9.1.3 softwares
Row variance analysis and test of significance.
As a result：
Experimental result is summarized in table 1.As shown in table 1, the striped rice borer one raised with apple feed
It is straight to keep larval stage, it is impossible to normally to pupate, sprout wings, lay eggs.Feed and hay prepared by embodiment l
White feed feeding effect is suitable, wherein male pupa weight and single female egg laying amount two indices in striped rice borer
On, feed prepared by embodiment l is significantly better than wild rice stem feed.Fig. 1-4 provides embodiment l preparations
The raising of feed and wild rice stem feed in different phase contrast photo.As Figure 1-4, embodiment is used
The striped rice borer pupa that l prepares feed raising is bigger on build, and spawning is more.
Table 1, the result of Three feed raising striped rice borer
Note：Data are to repeat the average ± standard deviation of experiment three times in table；* reached with the difference compareed
The level of signifiance (P<0.05).
Claims (10)
1. striped rice borer feed, it includes carrot prepared product.
2. striped rice borer feed as claimed in claim 1, also comprising glacial acetic acid.
3. striped rice borer feed as claimed in claim 1 or 2, also comprising soy meal, dusty yeast,
Any combination of casein, sucrose or more.
4. the striped rice borer feed as any one of claim 1-3, it, which includes to be selected from, has
Following components of following weight percentage：
In terms of the weight of raw material carrot, 7.5-22.5% carrot prepared product；
0.1-1% glacial acetic acid；
4.5-5.5% soy meal；
3-4% dusty yeast；
1.5-2% casein；
1.5-2% sucrose；
Or more any combination.
5. the striped rice borer feed as any one of claim 1-4, also include selected from following
Component：Agar, ascorbic acid, cholesterol, Choline Chloride, Wei Shi salt, B B-complex
B, any combination of sorbic acid, methyl p-hydroxybenzoate, formaldehyde and the above.
6. the preparation method of striped rice borer feed, comprises the following steps：
(1) homogenate is made in carrot and obtains carrot prepared product, add soy meal, dusty yeast,
Casein and sucrose, after stirring, autoclaving is carried out, obtains component A；
(2) water is added into agar and is heated to dissolving, is mixed with component A, after stirring
Cooling, obtains component B；
(3) by ascorbic acid, cholesterol, Choline Chloride, Wei Shi salt, vitamin B compound,
Sorbic acid and methyl p-hydroxybenzoate are dissolved in water, are mixed with component B, then with formaldehyde and ice
Acetic acid solution mixes.
7. method as claimed in claim 6, in addition to make the product cooling obtained from step (3)
The step of solidification.
8. the striped rice borer feed prepared by the method described in claim 6 or 7.
9. the method for striped rice borer is raised, including with two changes any one of claim 1-5
Snout moth's larva feed or the striped rice borer forage feed two prepared by the method described in claim 6 or 7 are changed
Snout moth's larva.
10. method as claimed in claim 9, including following one or more steps：
(1) newly hatched larvae is to pupating：The striped rice borer feed is placed in raising box, will just be incubated
Larva is placed on feed, and raising box is placed in incubator, was added or was changed every 2-5 days and raises
Material, until larvae pupation, the environmental condition in the incubator is 28 ± 1 DEG C of temperature, relatively
Humidity 70-80%, light application time 16L:8D；
(2) pupate to emergence：When Chilo spp larvae was developed to for 6 age, in other raising box
It is middle to add the feed and allow Chilo spp larvae to pupate, pupa is chosen after pupating, distinguished female
After hero, it is respectively put into culture dish, is placed in step (1) and is raised under same environmental conditions, until
Adult eclosion；
(3) sprout wings to spawning：Adult after emergence is put into transplanting the production of tillering stage rice seedlings
Mate and oviposit is carried out in ovum cage；
(4) ovum is to newly hatched larvae：The rice leaf that production has striped rice borer ovum is removed, is put into culture dish,
It is subsequently placed in step (1) and is developed under same environmental conditions, until larvae hatch.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507472A (en) * | 2009-03-19 | 2009-08-19 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
| CN104957398A (en) * | 2015-06-08 | 2015-10-07 | 云南大学 | Artificial feed for spodoptera litura larvae and raising method of spodoptera litura larvae |
-
2016
- 2016-05-09 CN CN201610302851.0A patent/CN107348252A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507472A (en) * | 2009-03-19 | 2009-08-19 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
| CN104957398A (en) * | 2015-06-08 | 2015-10-07 | 云南大学 | Artificial feed for spodoptera litura larvae and raising method of spodoptera litura larvae |
Non-Patent Citations (2)
| Title |
|---|
| 徐杨洋等: "水稻叶片全营养成分分析及在稻纵卷叶螟人工饲料研制中的应用", 《应用昆虫学报》 * |
| 温辉梁等: "《饲料添加剂生产技术与配方》", 31 December 2009, 江西科学技术出版社 * |
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