CN1073419A

CN1073419A - Multipurpose active enzyme preparation

Info

Publication number: CN1073419A
Application number: CN92104816A
Authority: CN
Inventors: 方祺霞
Original assignee: Individual
Current assignee: Individual
Priority date: 1992-06-29
Filing date: 1992-06-29
Publication date: 1993-06-23

Abstract

The present invention relates to a kind of multipurpose active enzyme preparation, include 13 kinds of wide spectrum respiratory enzymes (and isozyme), it forms 7 kinds of amino acid/11s.In farming, be used for crops such as paddy rice, corn, wheat, beans, melon, rape, cotton, fruit tree, herbage, fiber crops in the forestry, has the promotion plant growth, the double effect of controlling some insects, improve crop yield 15 to 20%, in livestock industry, make the fodder additives of chicken, duck, pig, ox etc., also have the promotion growth of animal, improve the effect of the output and the quality of its meat or egg, milk, but and instead of part imported fish meal.

This preparation production technique is simple, low production cost, and for the environment nontoxic residue-free, its comprehensive economy, ecology, social benefit are bigger.

Description

Multipurpose active enzyme preparation

(this preparation has been named in various places: multipurpose active enzyme preparation to the present invention relates to a kind of multipurpose active enzyme preparation of using in agricultural, forestry, the livestock industry; Plant Synergistic enzyme preparation; The paecilomyces fumosoroseus preparation; Green precious enzyme etc., its code name has: " 808 " and series product " 808-1 "), it can improve output and the quality of multiple green broad leaved plants such as grain, cotton, oil, fruit-bearing forest, herbage, flowers, can also improve the quantity that it produces meat or egg, milk and hair as the fodder additives of chicken, duck, pig and ox.The present invention also has an important purpose, and being provides a kind of double Biocidal preparation of controlling farming, woods insect for farming, forestry.

Current, farming, woods, the existing various plants sfgd. in animal husbandry aspect.The fifties so far, the means of prevention based on chemical pesticide and some biologies, physics of formation has been prevented and treated diseases and pests of agronomic crop, has developed production.But the life-time service chemical pesticide causes that pesticide residue constantly increase in the environment, ecologic disturbance, and people learn a lesson.And other vegetative practice coverage rate is little.Moreover behind the seventies, people have opened up short life and the dematuration of some chemical substance to plant, and are good as existing blade face treasured, multiple-effect, spray treasured, paclobutrazol and Jin Bangsu or the like, of a great variety.Though they have played production-increasing function on agricultural, but still the problem of unresolved its residual toxicity repeatedly uses because of its validity period short hairs again, and economic benefit is very not high, and can not doublely control the farmland insect pest.The present invention is directed to the problem of above-mentioned existence, created a kind of preparation of sheerly biological products, the needed zymin of organism of promptly synthesizing wide spectrum with the microorganism means, mainly show: (1) purposes is wide, it can be used as the growth regulator of multiple farming, woods crop, and the double some insects of controlling farming, woods of energy, the while also can be used as the fodder additives of livestock industry; (2) economic benefit height, validity period is long, and one batch of (or 1 year) crop is only used once, and the amplitude of volume increase can be 15 to more than 20%; (3) pollution-free and residual to environment food.

Goal of the invention:

The objective of the invention is to improve the farmland ecological environment quality, improve the output and the quality of agricultural-food with microorganism means protective plant.

Content of the present invention:

Content of the present invention: (1) improves the output and the quality of farming, forestry products, this preparation is biological products sheerly, and it can promote the photosynthesis of green broad leaved plant, improve the photosynthetic efficiency of plant leaf, play and promote growth, the effect that improves its output and quality.The output 15 that can improve crops such as paddy rice, wheat, corn, the seed of Job's tears (medicinal), millet, jowar, millet, sugarcane, beet, cotton, ramie, rape, soybean (comprising various beans), pears, apple, peach, Lee, jujube, lichee, longan, grape, melon, Chinese cabbage, herbage, tealeaves is to more than 20%; (2) this preparation belongs to a kind of microbial pesticide of insect pathogenic fungus again, 17 to 25 ℃ of average daily temperatures; In the environment of relative humidity 85%, can doublely control some insects in farming, the forestry, play the effect that reduces the chemical pesticide consumption; (3) this preparation is rich in the zymoprotein and the protein peptide of wide spectrum, can promote the various metabolic processes of organism, tentative experiment proves that it is again the fodder additives of a kind of biotype on the animal husbandry, it can improve the quantity of the product meat of chicken, duck, pig and ox or egg, milk, hair, remains further to be explored; (4) this preparation is investigated through the relevant security of national regulation, and it, is identified by security the person poultry safety to the environment nontoxic residue-free.

The problem that the present invention can solve:

The present invention if can be implemented widely, and then the problem that may solve in farming, woods, the animal husbandry is: (1) improve farming, woods on existing land area, herd and output and the quality of poultry, domestic animal, satisfies the growing material requisite of people; (2) residual quantity of chemical pesticide in the reduction food, the offspring benefits future generations.

Production of the present invention and using method are implemented by the following technical programs, and the production and the using method of this preparation are as follows:

1, preparation production method: it is imperfect fungi insect pathogenic fungus-paecilomyces fumosoroseus Beijing mutation (conventional bacterial strain) that (1) this preparation produces bacterium, expanding propagation under normal condition, solid of making or liquid seeds.Seed morphology is conidium or the hyphal fragment of this bacterium.Measure unit be hundred million/gram or hundred million/milliliter; (2) this preparation fermentation substratum is Semen Maydis powder (referring to the Semen Maydis powder that the corn of all kinds is milled and formed); (3) this preparation fermentation condition is, 20 to 24 ℃ of the temperature of material; Water content 65 to 70%; Inoculum size: in the ratio inoculation of 0.3 hundred million of every gram Semen Maydis powder breeding.Example: 100 kilograms of Semen Maydis powder, add seed 33,000 hundred million, add 65 to 70 kilograms in water, insert in the fermenter after stirring, 20 to 24 ℃ of condition bottom fermentations 50 to 60 hours, or reach 2 * 10 when the bacterium amount ⁹Stop fermentation during/gram; (4) air-dry: air-dry when fermented product stops fermentation, and wear into 30 to 40 purpose fineness (being Powdered), and packed standby.Figure of description is the production synoptic diagram of this preparation.

2, preparation using method: this preparation is a kind of meal, and when using on farm crop, the need thin up becomes the suspension of desired concn, is sprayed on the leaf portion of farm crop with compression sprayer.Its using method: (1) uses the position: mainly be used in the leaf back of plant, grass is used seedling stage, then is sprayed on the plant to get final product; (2) using dosage: grass every mu of using dosage in seedling stage is 100 to 120 grams, and cotton, rape working concentration are 0.9%; Xylophyta (as each planting fruit-trees) working concentration is 0.5 to 0.7%, and homogeneous year uses once; (3) the best use age: use early stage in plant growth, grass kind after 30 to 40 days, fruit tree is then spent back 20 to 30 days thanking, cotton kind after 30 to 40 days, rape should be when 5,6 leaves, cold ground herbage should be used when average daily temperature is more than 15 ℃; (4) use the effect example of 808 preparations:

Wheat: the individual plant small ear increases by 0.6; Effectively small ear increases by 0.7; Grain number per spike increases by 2,3; Mu spike number amount increases by 30,000 fringes; Thousand seed weight increases by 2 grams; Per mu yield increases by 88.4 kilograms.

Paddy rice: plant height increases by 3.5 centimetres; Spike length increases by 1.1 centimetres; Total real grain number increases by 4%; Total grain number increases by 6%; Total real grain heavily increases by 2%; Thousand seed weight reduces by 0.6 gram, and 390 kilograms of comparisons of per mu yield are according to increasing by 76.5 kilograms.

Corn: fresh weight increases by 10%; Dry weight increases by 36%.

Cotton: growth sampling and measuring result in mid-term, cotton boll increases by 36%; The total amount of cotton boll and flower increases by 38%.

Citrus: leaf is long to increase by 1.25 centimetres; 1.12 centimetres of the wide increases of leaf; The tip is long to increase by 7 centimetres; Single fruit weight increases by 10.9%; The sugar amount rises to 11.32 from 10.46.

Apple: promote the growth of nursery stock, press the ratio of mobile jib girth and its increment, the height of comparison photograph increases by 3%; The tip is long to increase by 43%, and the number of sheets increases by 36.The sugar amount increases by 1.6%.

Soybean: improve soybean yields 15%, and reduce the hazard ratio of its aphid and virus disease.

As the first test result of chicken feed addictive, new chicken egg productivity increases by 10%; Old chicken egg productivity increases by 20 to 30%, alternative part imported fish meal.

The biochemical property of this preparation:

It is zymoprotein that this preparation mainly contains effective constituent, its biochemical studies:

1, protein analysis: contain protein 7 to 10%, it forms amino acid 17 kinds.

2, enzyme and Isozyme Analysis thereof: main organized enzyme has two big classes: (1) wide spectrum respiratory enzymes: enzymic activity wherein is strong and stronger 9 kinds: aldehyde oxidase, acid phosphatase, esterase, FURAMIC ACID, bright aminopeptidase, malate dehydrogenase (malic acid dehydrogenase),, malic enzyme, tetrazolium oxydase and serum lactic dehydrogenase; Weak 4 kinds of enzymic activity: alcoholdehydrogenase, phosphoglycerol dehydrogenase, hydroxybutyric dehydrogenase and sorbito dehy drogenase.They are mostly relevant with the respiratory metabolism of plant, and that wherein have is exactly member in the tricarboxylic acid cycle enzyme system, and as malate dehydrogenase (malic acid dehydrogenase), it extensively is present in each tissue of plant.On plastosome, its energy catalysis oxysuccinic acid becomes the reaction of oxaloacetic acid; In tenuigenin, it can be reduced to oxysuccinic acid with oxaloacetic acid again, thereby is an important enzyme in the carbohydrate metabolism.Its reaction formula is:

Equally, FURAMIC ACID also is a member in the tricarboxylic acid enzyme system, can make citric acid become oxysuccinic acid.Serum lactic dehydrogenase is a kind of enzyme in the anaerobic metabolism, and it can become lactic acid to pyruvic acid; And for example esterase, bright amine peptase and acid phosphatase, these enzymes extensively exist in vivo and have participated in various reactions, and esterase also has detoxification.(2) broad-spectrum protease: A, protein and polypeptide: the protein and the polypeptide of visible this preparation respectively have more than 14 bands on electrophoretogram.And polypeptides matter has very vital role in vital movements such as nervous activity, immunomodulatory; B, amino acid kind: the conclusion that draws from amino acid composition analysis is: the amino acid of constitutive protein matter has 17 kinds; C, available amino acid kind: this preparation produces bacterium and can utilize 17 seed amino acids to form mycelium respectively on the washing agar plate, illustrates that the outer amino acid kind that can absorb of this thalline also is a wide spectrum.From A, B, three test-results explanations of C, protein synthesis of this preparation and lytic enzyme also all are wide spectrums.(3) produce the bacterium enzymatic property: the preparation enzymic activity is to depend on to produce the bacterium enzymatic property.A, respiratory enzyme: produce bacterium and have 11 kinds of respiratory enzymes, account for 85% of 13 kinds of respiratory enzymes of preparation; B, protein and polypeptide: protein and polypeptide class that the generation bacterium has reach more than 10 bands.Account for 75% of preparation 14 bands; C: total amino acid content: produce the bacterium total amino acid content and reach 16.4%, and the preparation total amino acid content is 7 to 10%.3 of A, B, C mainly depend on the biological characteristics that produces bacterium except that explanation preparation zymoprotein, also depend on the biological characteristics of substratum.

Above-mentioned test-results and analysis have disclosed this preparation enzyme class broad spectrum from the molecular biosciences level, present multi-functional result on the application practice thereby tentatively be illustrated in.

Realize best way of the present invention

808 multipurpose active enzyme preparations are by biosynthetic, and its preparation effect depends on the intensity of the biological characteristics that produces bacterium.Keep or strengthen the performance that produces bacterium carrying out the research work of planting.If be used for mass-produced seed by research unit's supply; Production unit is born the task of a large amount of cultivations.Then:

1, can strengthen or keep the activity of kind, strengthen the effect of this preparation.

2, production cost descends, as long as production department provides the expense of processes such as material, place and koji tray cultivation, natural air drying, plastic bag packaging.Can save installation cost expenditure from test tube → seeding tank or Kolle flask cultivation.

3, in such a way, seed all can be introduced in all agricultural, mountain area and poverty-stricken area, and fermentation is used voluntarily, and institute's create beneficial result is then bigger.

Claims

1, a kind ofly be applicable to farming, woods, herd and the already last multipurpose active enzyme preparation that uses of feed, form by 13 kinds of respiratory enzymes (and isozyme), it is characterized in that producing the biosynthesizing of bacterium paecilomyces fumosoroseus Beijing mutation (SP.808), by producing bacterium conidium 0.3 hundred million, Semen Maydis powder (referring to the Semen Maydis powder that all kinds corn grinds) 1 gram, the ratio that water is 0.7 milliliter is given amount, 28 to 30 ℃ of fermentations 50 to 60 hours, and the biotechnological formulation that obtains after air-dry, be used for having the promotion plant growth on the farm crop, the double long-acting of controlling crop pest, can improve the output and the quality of agricultural-food, and can reduce the pollution and the residual quantity of chemical pesticide, being used for feeding animals, is a kind of good fodder additives, can replace the part imported fish meal.

2, multipurpose active enzyme preparation according to claim 1, it is characterized in that being to comprise respiratory enzyme (and isozyme) (is acid phosphatase for 13 kinds, alcoholdehydrogenase, aldehyde oxidase, esterase, FURAMIC ACID, phosphoglycerol dehydrogenase, hydroxybutyric dehydrogenase, serum lactic dehydrogenase, malate dehydrogenase (malic acid dehydrogenase), malic enzyme, bright aminopeptidase, sorbito dehy drogenase, the tetrazolium oxydase) and protein peptide 14 bands, crude protein total amount 10%, it forms amino acid/11 (has aspartic acid for 7 kinds, Threonine, Serine, L-glutamic acid, proline(Pro), glycine, L-Ala, Gelucystine, Xie Ansuan, methionine(Met), Isoleucine, leucine, tyrosine, phenylalanine, Histidine, Methionin, arginine), as the probiotics of plant materials and the active substance of animal feedstuff additive, these features are different fully with other bacterial strain physiological characteristics of Paecilomyces varioti of registered patent.

3, zymin as claimed in claim 1, its formulation characteristics is light pink gray powder powder thing, applied range, as grain, cotton (comprising crudefiber crop), oil, really, vegetables, flowers and tealeaves etc., can be used as the fodder additivess of chicken, duck, pig, ox, fish, shrimp etc. in addition.