CN107333485A - Soil seed bank, soil seed pool sprouts device and application method - Google Patents

Abstract

The present invention relates to a kind of soil seed bank, soil seed pool to sprout device and application method.The soil seed bank, soil seed pool sprouts device and includes pallet, sponge and sprouting dish；The sponge of water suction is placed with pallet, sprouting dish is arranged on sponge；Sprouting dish is used for the soil for placing detection soil seed bank, soil seed pool；Through hole is provided with the bottom surface that sprouting dish contacts with sponge, the quantity of through hole is multiple, and through hole is used to by through hole diffuse up the moisture in sponge to the soil of sprouting dish.

Description

Soil seed bank germination device and method of use

technical field

The invention relates to the technical field of agricultural experiment devices, in particular to a soil seed bank germination device and a method for using it.

Background technique

The soil seed bank refers to the sum of all viable seeds in the soil and the fallen leaves on the soil surface, which is the material basis for the regeneration of plant communities. Identifying the types and quantitative characteristics of these seeds is a key issue in many related disciplines such as plant community and restoration ecology. important content.

One of the key technologies in the research process of soil seed bank is the determination of the number of species and the identification of species. There are two commonly used methods for species identification: one is to directly separate the weed seeds from the soil, and then count them, that is, the physical separation method (or called direct statistical method), this method includes elutriation method, blowing method, floating method, etc.; the second is to put the collected soil samples in a suitable germination environment to germinate as many seeds as possible, and identify these germination The type and quantity of seedlings are used to infer the type composition and density of the soil seed bank, which is called the seed germination method.

Because the elutriation and flotation methods require a lot of manpower and material resources, and are not accurate, they are rarely used in experiments. The seed germination method has irreplaceable advantages in detection precision and accuracy, and overcomes the disadvantage that some seeds are difficult to physically separate, and has been widely used at home and abroad. In general, the seed germination method requires spreading the soil sample in a flower pot with sterile sand as the substrate. The thickness of the soil sample is generally 1-2 cm, and it is placed in a suitable environment to allow it to germinate naturally. The moisture content of the soil. After 15-25 days, count and pull out the identified seedlings, continue watering, and continue the whole process until no more weed seedlings grow in the flowerpot, then stir the soil samples and continue to observe until the emergence stops. After repeating 3 times, the seed germination experiment was ended. The whole process took about 3 months. The operation time was too long, and during the experiment, when the soil surface was replenished with water, it was easy to cause soil compaction and damage the flatness of the soil surface, and even small seeds Washing out the soil layer will affect the germination of seeds, and it is difficult to control the thickness of 1-2cm when laying sand and other substrates at the bottom, and it is easy to stir up the lower layer of sand and other substrates, which will eventually reduce the repeatability of the experiment.

Contents of the invention

In order to improve the deficiencies of the prior art, the object of the present invention is to provide a soil seed bank germination device and its use method, to solve the existing in the prior art panning method and floating method to spend a lot of manpower and material resources, and inaccurate; The operation time of the seed germination method experiment is too long, and when the soil surface is replenished with water during the experiment, it is easy to cause soil compaction and damage the flatness of the soil surface, and even small seeds will be washed out of the soil layer, affecting seed germination, and sand is laid on the bottom It is difficult to control the thickness of 1-2cm when the substrate is stirred, and it is easy to stir up the substrate such as the sand in the lower layer, which eventually leads to the technical problem of reducing the repeatability of the experiment.

In an embodiment of the present invention, a soil seed bank germination device is provided, and the soil seed bank germination device includes a tray, a sponge and a germination plate; a water-absorbing sponge is placed in the tray, and the germination plate is arranged on the on the sponge; the germination plate is used to place and detect the soil of the soil seed bank; the bottom surface of the germination plate in contact with the sponge is provided with a through hole, the number of the through holes is multiple, and the through hole is used to Moisture in the sponge diffuses upwards into the soil of the germination tray through the through holes.

Preferably, the bottom surface of the germination tray is made of gauze, and the mesh of the gauze is between 60 mesh and 200 mesh.

Preferably, the gauze is nylon mesh.

Preferably, the shapes of the bottom projections of the tray, the sponge and the germination tray are the same, and the bottom projections of the germination tray and the sponge have the same size, and the bottom projection of the tray is larger than the bottom projection of the germination tray.

Preferably, the thickness of the sponge is 2-3 cm.

Preferably, the germination device of the soil seed bank further includes a watering mechanism, and the watering mechanism is used for watering the tray, so as to supplement the water lost by the sponge.

Preferably, the watering mechanism includes a water storage tank and a water outlet pipe; the water storage tank is provided with a water outlet, one end of the water outlet pipe communicates with the water outlet, and the other end of the water outlet pipe faces the inside of the tray, The outlet pipe is provided with a valve.

In an embodiment of the present invention, a method for using a soil seed bank germination device is also provided, comprising the following steps:

S1. The soil sample is sieved to remove impurities such as plant roots and stones in the soil sample;

S2. The soil sample after sieving is tiled on the bottom of germination dish;

S3. Put the sponge in the tray and add an appropriate amount of water to ensure that the sponge absorbs enough water but does not flow out, and then place the germination tray on the sponge;

S4. Use a weighing device to weigh the germination device of the soil seed bank, and record the initial weight;

S5. According to the initial weight of the germination device of the soil seed bank, the water loss of the soil is replenished, and when the water is replenished, it is directly added to the tray, and the amount of water added is the difference between the existing weight and the initial weight;

S6. Start seedling identification after the seeds germinate.

Preferably, the thickness of the soil sample tiled in step S2 is 2 cm.

Preferably, the method for using the soil seed bank germination device also includes performing after S5:

S50. Adding a nutrient solution into the tray, the nutrient solution is used to promote the growth of the seedlings.

The soil seed bank germination device provided by the present invention comprises a tray, a sponge and a germination plate; a water-absorbing sponge is placed in the tray, and the germination plate is arranged on the sponge; the germination plate is used to place and detect soil The soil of the seed bank; the bottom surface of the germination plate in contact with the sponge is provided with through holes, the number of the through holes is multiple, and the through holes are used to diffuse the moisture in the sponge upwards to germinate through the through holes soil in the tray. When the experimenter uses the soil seed bank germination device provided by the invention, the experimenter first sieves the soil sample, removes impurities such as plant rhizomes and stones in the soil sample, and spreads the soil on the bottom surface of the germination tray with a thickness of 2 cm; put the sponge in the tray and add an appropriate amount of water to ensure that the sponge absorbs enough water but does not flow out, then place the germination tray on the sponge; at this time, use a weighing device to weigh the germination device of the soil seed bank, and record the initial Weight: the soil seed bank germination device was placed in a germination place with suitable conditions for the experiment. During the experiment, the soil lost water was replenished according to the initial weight of the soil seed bank germination device. When replenishing water, it was directly added to the tray. The amount of water added It is the difference between the current weight and the initial weight; seedling identification starts after the seeds germinate. Compared with the existing soil seed bank experiment method, the combination of the through hole on the bottom surface of the germination plate adopted by the soil seed bank germination device provided by the invention in combination with the sponge realizes the bottom replenishment of soil moisture and the flatness of the soil surface, which can facilitate The addition of water is realized, and at the same time, such operation will not overturn the seeds, and it is not easy to cause soil surface compaction, which is beneficial to seed germination and improves the distinguishability of seedlings; the sponge can be reused after being cleaned and sterilized, so the soil seed bank provided by the invention can germinate The device is inexpensive to manufacture and is reusable.

In the method for using the soil seed bank germination device provided by the invention, the experimenter first sieves the soil sample, removes impurities such as plant rhizomes and stones in the soil sample; spreads the sieved soil sample on the bottom of the germination tray Place the sponge in the tray and add an appropriate amount of water to ensure that the sponge absorbs enough water but does not flow out, and then place the germination tray on the sponge; at this time, use a weighing device to weigh the germination device of the soil seed bank, and record the initial weight ;During the experiment, according to the initial weight of the germination device of the soil seed bank, the water lost in the soil was replenished, and when the water was replenished, it was directly added to the tray, and the amount of water added was the difference between the existing weight and the initial weight; after the seeds germinated, the Seedling identification. Compared with the existing soil seed bank experimental method, the combination of the through hole on the bottom surface of the germination plate adopted by the soil seed bank germination device provided by the invention in conjunction with the sponge has realized the bottom replenishment of soil moisture and the smoothness of the soil surface, and at the same time The operation will not overturn the seeds, and it is not easy to cause compaction of the soil surface, which is beneficial to the germination of the seeds and improves the distinguishability of the seedlings; the sponge can be reused after being washed and sterilized, so the manufacturing cost of the soil seed bank germination device provided by the present invention is low, and reusable.

Description of drawings

In order to more clearly illustrate the specific implementation of the present invention or the technical solutions in the prior art, the following will briefly introduce the accompanying drawings that need to be used in the specific implementation or description of the prior art. Obviously, the accompanying drawings in the following description The drawings show some implementations of the present invention, and those skilled in the art can obtain other drawings based on these drawings without any creative work.

Fig. 1 is the front view of the soil seed bank germination device provided by the present invention;

Fig. 2 is the structural representation of the soil seed bank germination device provided by the present invention;

Fig. 3 is the top view of the soil seed bank germination device provided by the present invention;

Fig. 4 is a schematic structural view of the germination tray of the soil seed bank germination device provided by the present invention.

Icons: 1—germination device of soil seed bank; 10—tray; 20—sponge; 30—germination tray; 300—through hole.

detailed description

The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer ", etc., the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation , constructed and operated in a particular orientation and therefore should not be construed as limiting the invention. In addition, the terms "first", "second", "third" and the like are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that unless otherwise specified and limited, the terms "communication" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integrated Ground connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediary, and it can be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention in specific situations.

The present invention provides a soil seed bank germination device 1 and its use method, and provides its implementation.

As shown in Figures 1-4, the soil seed bank germination device 1 provided by the present invention includes a tray 10, a sponge 20 and a germination disc 30; a water-absorbing sponge 20 is placed in the tray 10, and the germination disc 30 is arranged on the sponge 20 The germination plate 30 is used to place and detect the soil of the soil seed bank; the bottom surface of the germination plate 30 in contact with the sponge 20 is provided with a through hole 300. Moisture diffuses upwards into the soil of the germination tray 30 through the through holes 300 . When the experimenter uses the soil seed bank germination device 1 provided by the present invention, the experimenter first sieves the soil sample, removes impurities such as plant rhizomes and stones in the soil sample, and spreads the soil on the bottom surface of the germination tray 30 with a thickness of 2 cm; put the sponge 20 in the tray 10 and add an appropriate amount of water to ensure that the sponge 20 absorbs enough water but does not flow out, and then the germination plate 30 is placed on the sponge 20; at this time, the soil seed bank germination device 1 is carried out using a weighing device Weigh and record the initial weight; the germination device 1 of the soil seed bank is placed in a germination place with suitable conditions for experimentation. During the experiment, the soil loss moisture is replenished according to the initial weight of the germination device 1 of the soil seed bank. Add in the tray 10, the amount of water added is the difference between the existing weight and the initial weight; start to identify the seedlings after the seeds germinate. Compared with the existing soil seed bank experiment method, the combination of the through hole 300 on the bottom surface of the germination plate 30 adopted by the soil seed bank germination device 1 provided by the present invention in combination with the sponge 20 realizes the lower replenishment of soil moisture and the smoothing of the soil surface degree, can realize the addition of moisture conveniently, and at the same time, such operation will not overturn the seeds, is not easy to cause soil surface compaction, is beneficial to seed germination, and improves the distinguishability of seedlings; the sponge 20 can be reused after cleaning and sterilization, so the present invention provides The soil seed bank germination device 1 has low manufacturing cost and can be used repeatedly.

The bottom surface of the germination tray is made of gauze, and the mesh number of the gauze is between 60 mesh and 200 mesh. The mesh number range of setting gauze is in order to limit the aperture range of through hole 300 in a certain range, if the aperture is too large, the soil in the germination disc 30 will be dropped out of the germination disc 30 from the through hole 300; Small, it may cause the root of the seed in the soil to be unable to protrude from the through hole 300 after germination, so that it is not easy to absorb water, so that the germinated seed dies.

The gauze can be a nylon mesh. Nylon mesh is a chemical fiber woven mesh made of chemical fibers such as nylon, nylon, polyester, and nalon, which are machined through wire mesh. Jinlun has the function of high temperature and alkali resistance, polyethylene has the effect of acid resistance, nylon mesh is the general name of these chemical fiber woven mesh. Using the nylon net as the gauze can reduce the manufacturing cost of the soil seed bank germination device 1 provided by the present invention. It should be noted that the material of the gauze net is not limited to nylon net, it can also be stainless steel net and the like.

The bottom surface projections of the tray 10, the sponge 20, and the germination plate 30 have the same shape, and the bottom surface projections of the germination plate 30 and the sponge 20 have the same size, and the bottom surface projection of the tray 10 is larger than the bottom surface projection of the germination plate 30. Specifically, the germination disc 30 can be a disc with a diameter of 10-15 cm or a rectangular disc with an area equivalent to a disc of 10-15 cm. The tray 10 is made of PVC material or metal material. The shape of the tray 10 is the same as that of the germination disc 30. When the germination dish 30 was a disc, the tray 10 was a disc whose diameter was 1 cm larger than the germination dish 30; A rectangular dish with sides measuring 1 cm. The thickness of the sponge 20 can be 2-3 centimeters; the height of the tray 10 is 4-8 centimeters, and the height of the germination plate 30 is 4-7 centimeters.

The soil seed bank germination device 1 also includes a watering mechanism, which is used for watering the tray 10 , so as to supplement the water lost by the sponge 20 . Specifically, the watering mechanism may include a water storage tank and a water outlet pipe; the water storage tank is provided with a water outlet, one end of the water outlet pipe communicates with the water outlet, the other end of the water outlet pipe faces the inside of the tray 10, and the water outlet pipe is provided with a valve. When in use, the water in the water storage tank can be directly introduced into the tray 10 through the water outlet pipe, which saves the time of the experimenter.

In the method for using the soil seed bank germination device 1 provided by the present invention, the experimenter first sieves the soil sample to remove impurities such as plant rhizomes and stones in the soil sample; then places the sponge 20 in the tray 10 and adds an appropriate amount of water to ensure The sponge 20 absorbs enough water but does not flow out, and then the germination tray 30 is placed on the sponge 20; at this time, the soil seed bank germination device 1 is weighed with a weighing device, and the initial weight is recorded; The initial weight of the device 1 is to replenish the water lost in the soil. When replenishing water, it is directly added to the tray 10. The amount of water added is the difference between the current weight and the initial weight; the seedlings are identified after the seeds germinate. Compared with the existing soil seed bank experiment method, the combination of the through hole 300 on the bottom surface of the germination plate 30 adopted by the soil seed bank germination device 1 provided by the present invention in combination with the sponge 20 realizes the lower replenishment of soil moisture and the smoothing of the soil surface At the same time, this operation will not overturn the seeds, is not easy to cause soil surface compaction, is beneficial to seed germination, and improves the distinguishability of seedlings; the sponge 20 can be reused after cleaning and sterilization, so the soil seed bank germination device 1 provided by the present invention The production cost is low, and can be used repeatedly.

Preferably, the thickness of the soil sample tiled in step S2 is 2 cm. Moreover, the method of using the soil seed bank germination device 1 also includes following S5: S50. Adding nutrient solution into the tray 10, the nutrient solution is used to promote the growth of seedlings. The nutrient solution can be selected from Hoagland's nutrient solution. Hoagland's solution is the most commonly used formula in plant nutrient solution, which is beneficial to plant reproduction, growth and development. During the experiment, the experimenters observed the emergence of seedlings in the germination device 1 of the soil seed bank. When some seedlings emerged, they could change to add 1/4 concentration of Hoagland's improved nutrient solution (without adding trace elements) when adding water. , for unidentifiable seedlings, let them continue to grow until the species of seedling can be determined.

In summary, the soil seed bank germination device 1 provided by the present invention includes a tray 10, a sponge 20 and a germination plate 30; a water-absorbing sponge 20 is placed in the tray 10, and the germination plate 30 is arranged on the sponge 20; the germination plate 30 is used to place and detect the soil of the soil seed bank; the bottom surface of the germination plate 30 in contact with the sponge 20 is provided with a through hole 300, the number of the through hole 300 is multiple, and the through hole 300 is used to pass the moisture in the sponge 20 through the through hole 300 diffuses upwards into the soil of the germination tray 30. When the experimenter uses the soil seed bank germination device 1 provided by the present invention, the experimenter first sieves the retrieved soil sample with a soil sieve with an aperture of 2mm, fully mixes the soil sample in the same spot, and repeats 5 times in the experiment On germination plate 30, tile soil sample again, thickness is 2 centimetres; Sponges 20 are placed in tray 10 and add appropriate amount of water to ensure that sponge 20 absorbs enough moisture but not outflow, and the germination plate 30 that tiles have soil sample is placed simultaneously Put it on the sponge 20; at this time, use a weighing device to weigh the germination device 1 of the soil seed bank, and record the initial weight; place the germination device in a light incubator or a place suitable for seed germination, with a daytime temperature of 25-35°C and a temperature of 25-35°C at night. 15-20°C; During the experiment of the soil seed bank germination device 1, the water lost in the soil was supplemented according to the initial weight recorded before the experiment, and the water was replenished once every 3 days. The amount is the difference between the existing weight and the initial weight; observe the emergence of the seedlings in the germination device 1 of the soil seed bank, and when the seedlings are unearthed, you can change to add 1/4 concentration of Hoagland's improved nutrient solution when adding water (without adding trace elements), for unidentifiable seedlings, let them continue to grow until the type of seedling can be determined. Compared with the existing soil seed bank experiment method, the combination of the through hole 300 on the bottom surface of the germination plate 30 adopted by the soil seed bank germination device 1 provided by the present invention in combination with the sponge 20 realizes the lower replenishment of soil moisture and the smoothing of the soil surface degree, can realize the addition of moisture conveniently, and at the same time, such operation will not overturn the seeds, is not easy to cause soil surface compaction, is beneficial to seed germination, and improves the distinguishability of seedlings; the sponge 20 can be reused after cleaning and sterilization, so the present invention provides The soil seed bank germination device 1 has low manufacturing cost and can be used repeatedly.

In the method for using the soil seed bank germination device 1 provided by the present invention, the experimenter first sieves the soil sample to remove impurities such as plant rhizomes and stones in the soil sample; then places the sponge 20 in the tray 10 and adds an appropriate amount of water to ensure The sponge 20 absorbs enough water but does not flow out, and then the germination tray 30 is placed on the sponge 20; at this time, the soil seed bank germination device 1 is weighed with a weighing device, and the initial weight is recorded; The initial weight of the device 1 is to replenish the water lost in the soil. When replenishing water, it is directly added to the tray 10. The amount of water added is the difference between the current weight and the initial weight; the seedlings are identified after the seeds germinate. Compared with the existing soil seed bank experiment method, the combination of the through hole 300 on the bottom surface of the germination plate 30 adopted by the soil seed bank germination device 1 provided by the present invention in combination with the sponge 20 realizes the lower replenishment of soil moisture and the smoothing of the soil surface At the same time, this operation will not overturn the seeds, is not easy to cause soil surface compaction, is beneficial to seed germination, and improves the distinguishability of seedlings; the sponge 20 can be reused after cleaning and sterilization, so the soil seed bank germination device 1 provided by the present invention The production cost is low, and can be used repeatedly.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.

Claims (10)

1. a kind of soil seed bank, soil seed pool sprouts device, it is characterised in that the soil seed bank, soil seed pool sprouts device and includes pallet, sponge And sprouting dish；

The sponge of water suction is placed with the pallet, the sprouting dish is arranged on the sponge；

The sprouting dish is used for the soil for placing detection soil seed bank, soil seed pool；

Through hole is provided with the bottom surface that the sprouting dish contacts with sponge, the quantity of the through hole is multiple, and the through hole is used for Moisture in sponge is diffused up to the soil of sprouting dish by the through hole.

2. soil seed bank, soil seed pool according to claim 1 sprouts device, it is characterised in that the bottom surface of the sprouting dish is by gauze Form, the mesh number of the gauze is in 60 mesh between 200 mesh.

3. soil seed bank, soil seed pool according to claim 2 sprouts device, it is characterised in that the gauze is nylon wire.

4. soil seed bank, soil seed pool according to claim 1 sprouts device, it is characterised in that the pallet, sponge and sprouting dish Bottom surface projection shape it is identical, and the sprouting dish is identical with the bottom surface projected size of sponge, the bottom surface projection of the pallet Bottom surface more than the sprouting dish projects.

5. soil seed bank, soil seed pool according to claim 1 sprouts device, it is characterised in that the thickness of the sponge is 2-3 lis Rice.

6. soil seed bank, soil seed pool according to claim 1 sprouts device, it is characterised in that the soil seed bank, soil seed pool sprouts device Also include watering mechanism, the watering mechanism is used to water into the pallet, so as to supplement the moisture of the sponge missing.

7. soil seed bank, soil seed pool according to claim 6 sprouts device, it is characterised in that the watering mechanism includes storage tank And outlet pipe；

Delivery port is provided with the storage tank, one end of the outlet pipe is connected with delivery port, the outlet pipe it is another Hold towards inside the pallet, valve is provided with the outlet pipe.

8. the soil seed bank, soil seed pool described in a kind of any one of claim 1-7 sprouts the application method of device, it is characterised in that bag Include following steps：

S1. pedotheque is sieved, the impurity such as the plant roots and stems in pedotheque and stone is removed；

S2., pedotheque after sieving is laid in the bottom of sprouting dish；

S3. sponge is positioned in pallet and adds amount of water and ensure that sponge suctions moisture but do not outflowed, then sprouting dish is placed In on sponge；

S4. sprout device to the soil seed bank, soil seed pool using weighing device to weigh, and record initial weight；

S5. the initial weight of device is sprouted according to soil seed bank, soil seed pool, soil loss moisture is supplemented, it is direct when keeping the skin wet Added into pallet, moisture addition is existing weight and the residual quantity of initial weight；

S6. seedling identification is proceeded by after germination.

9. soil seed bank, soil seed pool according to claim 8 sprouts the application method of device, it is characterised in that soil in step S2 Sample tiling thickness is 2 centimetres.

10. soil seed bank, soil seed pool according to claim 8 sprouts the application method of device, it is characterised in that the soil species The application method that word bank sprouts device is additionally included in what S5 was carried out afterwards：

S50. nutrient solution is added into the pallet, the nutrient solution is used for the growth for promoting seedling.