CN107325157A - Adjust the peptide of sebum secretion and the cosmetics comprising it - Google Patents
Adjust the peptide of sebum secretion and the cosmetics comprising it Download PDFInfo
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- CN107325157A CN107325157A CN201710665862.XA CN201710665862A CN107325157A CN 107325157 A CN107325157 A CN 107325157A CN 201710665862 A CN201710665862 A CN 201710665862A CN 107325157 A CN107325157 A CN 107325157A
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- peptide
- cosmetics
- extract
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- sebum secretion
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/671—Vitamin A; Derivatives thereof, e.g. ester of vitamin A acid, ester of retinol, retinol, retinal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
Abstract
The present invention relates to the active peptide with regulation sebum secretion effect and the cosmetics comprising it.Specifically, the invention discloses SEQ ID NO:1 and SEQ ID NO：2 peptide is used singly or in combination to adjust the activity of sebum secretion and includes the cosmetics of the peptide, wherein the cosmetics are included with weight %：Retinol 2.0 4.0, capsule of weeping forsythia berry extract 0.5 2.5, Salvia root P.E 0.5 2.5, anemarrhena asphodefoides extract 2.0 4.0, white willow bark extract 4.0 6.0,1,3 propane diols 2.0 4.0, shrubby sophora extract 4.0 6.0, soybean lecithin 2.0 4.0, glucan 2.0 4.0, niacinamide 1.0 2.0 and lauroyl arginine ethyl ester 2.0 4.0.The cosmetics of the present invention have the effects such as suppressing sebum secretion, treatment acne.
Description
Technical field
The present invention relates to regulation sebum secretion effect non-natural peptide and and they in cosmetic and personal nursing group
Application in compound.
Background technology
Normal skin contains the appendicle structure such as hair follicle, sebaceous glands, sweat gland.Sebaceous glands (sebaceousgland) is skin
Important appendicle structure in skin, is located between hair follicle and arrectores pilorum, is acinous gland, by the acinus of one or several cryptomeres mostly
Constituted with a common tubulature；Conduit is stratified squamous epithelium, is opened on hair follicle epimere more, and also some are directly open in skin
Skin surface.The distribution of sebaceous glands spreads all over except palm and sole outer skin Zone Full.The secretion of sebaceous glands is sebum, is 1,
The mixture of the lipids such as the ester of 3 propane diols three, aliphatic acid, wax ester, sterol ester, with skin care, reduces skin water loss, tune
Save the effects such as skin natural microbial composition.
Sebaceous glands has two kinds, and a kind of is the sebaceous glands being connected in pilosebaceous follicle with hair follicle, and another is to be individually present
Sebaceous glands.Sebaceous glands exist in eyelid, nose, penis, nymphae, the interior mucous membrane of cheek and nipple without hair-fields.
The amount of smegma sebum is by human androgen's level, the regulation of nutrition condition and mood.When these factors
Imbalance, while the pore on skin is blocked by dirt, adds hormone secretion disorder, causes follicular keratosis, further aggravate hair follicle
Block, acne will be caused to be formed, when bacterium breeds in the sebum of alluvial, trigger inflammation, acne will be formed and (be commonly called as the youth
Acne), cause skin damaged.
Scalp sebaceous glands excessive secretion sebum can cause oily hair, even result in generation dandruff.Dandruff is a kind of scalp
Imbalance, is characterised by the abundant loose paster of sheet, generally along with itch.The exacerbation furfur of scalp can develop into seborrheica
Dermatitis, it is a kind of serious dandruff with inflammation and erythema.
United States Patent (USP) 6,488,318, which is disclosed, uses 1,2,3,4- tetrahydrochysene -1,1,4,4- tetramethyls -6- [Alpha-Methyl benzene second
Alkenyl] naphthalene reduces the method and composition of sebum secretion as active component, and use it for preventing or treat by excess sebum
Disease caused by secretion, such as acne or oiliness scalp and hair.
United States Patent (USP) 11,991,587 discloses cosmetic use, by the former barberry of at least one in addition to jamaicin
Alkali/OPC, or the plant extracts containing it are administered on skin, suppress the activity of pilosebaceous unit as active material.
Also disclose the cosmetic composition containing it.
United States Patent (USP) 8,298,735 discloses a kind of method for treating disease caused by human and animal's disease of sebaceous glands,
It includes the composition that the reactive compound comprising smegma thing amount of suppression is applied to the humans and animals, reactive compound
Include acyl-coenzyme a cholesterol acyltransferase (ACAT) inhibitor or its prodrug.
Chinese patent CN105560128 discloses a kind of composition with suppression sebum secretion effect and application.The group
Compound is made up of following components：Shale oil sulphonic acid ester sodium, water-soluble tea tree oil, Chinese medicine composition, deionized water；Wherein Chinese medicine group
Compound is prepared by chrysanthemum indicum, granatum, honeysuckle, lucid asparagus, cactus.Said composition can effectively reduce skin oil and fat secretion,
Continuing to use persistently can control skin oil and fat to secrete, and non-stimulated to skin safe, in oil-control and acne-eliminating cosmetic, having
The oil-control of good Kazakhstan and acne-removing.
Chinese patent CN103458908, which is disclosed, a kind of is used to reducing that sebum in sebaceous glands to produce includes guava fruit
The composition of extract or card slave's card leaf extract or both, its can reduce the sebum amount of sebaceous glands generation, make pore unimpeded with
And processing acne.
These problems of acne, oily hair, dandruff, seborrhea, although not being serious health problem, its meeting pair
Personal image can cause great harmful effect, and serious psychological burden is caused to patient, have a strong impact on the work and life of patient, because
It is all significant to personal health and social development that this solves acne, oily hair, dandruff, these problems of seborrhea.
Often treated at present using the medicine containing antibiotic or hormone or cosmetics, but these medicines are while treatment
Often come stimulation and some harmful effects to skin tape, short is often had a rebound again after being discontinued, and long-term use is led
Chapped skin is caused even to produce drug resistance.So, by balance sebum secretion, build skin health microenvironment come solve acne,
The problems such as oily hair, dandruff, seborrhea, be desired at present.
Invention summary
It is new type of peptides of the exploitation with regulation sebum secretion effect, present inventor has performed insistent research and screens
Peptide library.Finally, the inventor has discovered that the new type of peptides with excellent regulation sebum secretion effect, final to realize the present invention
Purpose.
One object of the present invention has the peptide of regulation sebum secretion effect to provide.
Another object of the present invention is a kind of composition for being used to adjust sebum secretion of offer.
A further object of the present invention is a kind of method for adjusting sebum secretion of offer.
To realize the purpose of the present invention, the first aspect of the present invention is there is provided peptide, and it is selected from following peptide：
(i) peptide, it includes or is made up of following amino acid residue：
Ser-Val-Ala-Lys-Arg-Glu-Cys-Arg-Asp-Gln(SVAKRECRDQ)(SEQ ID NO:1, also referred to as
For " peptides of Peptide 07 ")；Or
(ii) peptide, it includes or is made up of following amino acid residue：
Glu-His-Gly-Pro-Phe-Asp-Ala-Gln-Pro(EHGPFDAQP)(SEQ ID NO:2)。
According to the preferred aspect of the present invention there is provided the combination of peptide, it includes SEQ ID NO:1 and SEQ ID NO:2
Peptide combination.
According to the second aspect of the invention there is provided regulation sebum secretion composition, it includes the SEQ ID of the present invention
NO:1 peptide and cosmetically acceptable carrier.
According to the preferred aspect of the present invention there is provided regulation sebum secretion composition, it includes the SEQ ID of the present invention
NO:1 and SEQ ID NO：2 peptide combination and cosmetically acceptable carrier.
There is provided the SEQ ID NO of the present invention according to the third aspect of the invention we:1 peptide is used to adjust sebum secretion
Purposes.
There is provided the SEQ ID NO of the present invention according to a preferred aspect of the present invention:1 and SEQ ID NO：2 peptide combination is used
In the purposes of regulation sebum secretion.
The present invention's additionally provides a kind of cosmetics for being used to adjust sebum secretion, and it is included：
(a) single peptide of the invention or peptide combination；
(b) antioxidant；
(C) NMF；
(d) vegetable oil；
(e) lecithin；
(f) preservative；
(g) essential oil；With
(h) purified water.
Following detailed description of the invention is being read, including after exemplary embodiment and embodiment, this hair
Bright these and other aspects will become obvious for those skilled in the art.
It is described in detail
Two peptides are claimed in the present invention, that is, with SEQ ID NO:1 peptide (i) or with SEQ ID NO:2
Peptide (ii).When these peptides are used to adjust sebum secretion composition, the suppression provides the benefit in terms of regulation sebum secretion.
Additionally by experiment, not only confirm that each self energy of the two peptides reduces sebum secretion, and be also noted that they
Cooperative interaction when being used together.
As used herein, it is intended to that there are them in the general of this area unless otherwise specifically defined all terms
Implication.Term " amino acid " is intended to include naturally occurring amino acid and non-naturally occurring amino acid, broadly including institute
Have with carboxyl and at least one can be formed peptide bond primary or secondary amine groups small molecule.Term " peptide " is intended to include all tools
There is the molecule of at least one peptide bond, therefore including dipeptides, tripeptides, oligopeptides and the polypeptide for being up to about 20 amino acid residues.
Term " peptide " also include with one or more linkers, interval base or terminal groups, be not the structure of amino acid.
The peptide of the present invention is provided in cosmetically acceptable carrier.Carrier can be hydrophobic or hydrophilic.It is suitable
When hydrophobic carrier include for example, in cosmetics commonly use waxy nonionic, the ester and ether of such as fatty alcohol and aliphatic acid, carbon
Chain length is C4 to C22, most preferably preferably C8 to C18, C12 to C18.
The example of fatty hydrophobic carrier includes isopropyl myristate, isopropyl palmitate, octyl palmitate, lanolin fatty acid
Isopropyl ester, acetulan, the benzoic ether of C12-C15 alcohol, Octanoic acid, hexadecyl ester, cetin, myristic acid meat
Cardamom ester, Tetradecyl lactate, acetic acid cetyl, two caprylic/capric propylene glycol esters, decyl oleate, acetylated lanolin, enanthic acid
Stearate, malic acid diisopstearate, octyl hydroxystearate, octyl hydroxystearate, isopropyl isostearate etc..
Appropriate hydrophilic support solution can be, such as the glycol for the alkoxylate commonly used in glycol and cosmetics, including
Ethylene glycol, diethylene glycol, triethylene glycol, propane diols, DPG etc..
Regulation sebum secretion composition can be configured to nutrient solution, Essence, cosmetics, emulsifiable paste, toner, slurries, spray
Mist agent, stylus and other forms well known by persons skilled in the art.Emulsifiable paste and toner are currently preferred product forms.
In cosmetically acceptable carrier, the concentration range of peptide can be 1 μM to 200 μM, preferably 10ppb to 150 μM,
More preferably 20 μM to 100 μM, most preferably 36 μM to 50 μM.
Regulation sebum secretion composition typically comprises above-mentioned carrier solution, and its level is about 0.01% to about 90% weight
Amount, preferably from about 0.1% to about 50% weight, more preferably from about 0.1% to about 20% weight, most preferably from about 1% to about 10% weight.
As antioxidant, such as butylated hydroxyanisole (BHA), dibutyl hydroxy toluene, sodium hydrogensulfite, different anti-can be enumerated
Bad hematic acid and its salt, glutathione, glutathione peroxidase, glutathione-S-transferase, catalase, superoxides
Mutase, thioredoxin, taurine, thiolaurine, hypotaurine, vitamin E etc..Wherein, preferably glutathione, dimension life
Plain E and catalase.These antioxidants can use a kind or two or more is applied in combination, preferably glutathione and dimension
Raw element E combination.In the composition for external application (such as cosmetics) of the present invention during addition antioxidant, its adding proportion does not have
It is particularly limited to, but relative to the total amount of composition for external application, usually 0.001~6 weight %, preferably 0.01~3 weight
Measure %, more preferably 0.2~2 weight %.
As NMF, such as alanine, serine, leucine, isoleucine, threonine, glycine, dried meat ammonia can be enumerated
The amino acid such as acid, hydroxy-proline, aspartic acid, arginine, theanine and its derivative；Ethylene glycol, 1,3-PD, 1,3-
The polyalcohols such as butanediol, diethylene glycol (DEG), triethylene glycol, tetraethylene glycol, DPG, 2 1,3 propane diols, polyethylene glycol；Ethylene glycol list first
Base ether, ethylene glycol monomethyl ether, ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, TC, diethylene glycol
Single propyl ether, diethylene glycol monobutyl ether, propylene glycol monoethyl, propylene glycol monopropyl ether, dihydroxypropane single-ethyl ether, dipropyl
The glycol ethers such as glycol list propyl ether；The sugar alcohols such as mannitol, D-sorbite, erythrite, xylitol, trehalose；Lecithin, hydrogenation ovum
The phosphatide such as phosphatide；The mucopolysaccharides such as Sodium Hyaluronate, acetyl group Sodium Hyaluronate, hyparinoids from animal organs, sodium chondroitin sulfate；Gelatine
The high-molecular compounds such as original, elastin laminin, keratin, chitin, chitosan, gelatin, polyglutamic acid；Sodium lactate, pyrrole
The compositions such as pyrrolidone carboxylic acid's sodium；Camomile extract, aloe extract, aloe vera extract, hamamelis extract, fan change
Plant extracts such as fragrant extract, thyme extract, tea extraction, extractive of perilla etc..Wherein, preferably glycine, 1,3 third
Glycol, DPG, 2 1,3 propane diols, TC, Sodium Hyaluronate, acetyl group Sodium Hyaluronate, pyrrolidines
Keto carboxylic acid sodium, polyglutamic acid.These NMFs can use a kind or two or more is applied in combination, preferably 1,3-PD and
Sodium Hyaluronate.In the composition for external application (such as cosmetics) of the present invention during addition NMF, its adding proportion does not have
It is particularly limited to, but relative to the total amount of composition for external application (such as cosmetics), usually 0.001~40 weight %, preferably
For 0.1~30 weight %, more preferably 5~20 weight %.
As vegetable oil, it is selected from rose hip oil, Sweet Almond Oil, rose hip oil, SIMMONDSIA CHINENSIS SEED OIL, wheat-germ oil, carrot
Oil, Avocado Oil, castor oil, avocado oil, coconut oil, palm oil, cupu oil, cocoa butter, palm-kernel oil, soybean oil, rice bran oil, reed
Luxuriant growth oil, mother-in-law Luo Yue purports, mowrah fat, sher butter, peanut oil, corn oil, macadimia nut oil, candlenut oil, husky thorn oil, flax
Oil, passionflower caul-fat, pot marigold, mango kernel oil, cynarrhodion oil, preferably Sweet Almond Oil, jojoba oil, one kind of aloe or
The two or more combination of person.Preferably, the vegetable oil in composition of the invention is rose hip oil.In the external preparation for skin of the present invention
In composition (such as cosmetics) during addition vegetable oil, its adding proportion is not particularly limited, but relative to external preparation for skin combination
The total amount of thing (such as cosmetics), usually 0.001~10 weight %, preferably 0.5~8 weight %, more preferably 1.0
~8.0 weight %.
As preservative, it is selected from phenmethylol, benzyl carbinol, Phenoxyethanol and lauroyl arginine ethyl ester, preferably bay
Acyl arginine ethyl ester.In the composition for external application (such as cosmetics) of the present invention during addition preservative, its adding proportion does not have
It is particularly limited to, but relative to the total amount of composition for external application (such as cosmetics), usually 0.001~5 weight %, preferably
For 0.1~5 weight %, more preferably 0.2~3 weight %.
As lecithin, it is preferably soybean lecithin.In the composition for external application (such as cosmetics) of the present invention
When adding lecithin, its adding proportion is not particularly limited, but relative to the total amount of composition for external application (such as cosmetics),
Usually 0.01~5 weight %, more preferably preferably 0.1~5 weight %, 0.2~2 weight %.
Preferably, glutathione 2.0-4.0, vitamin E 0.5-2.5, the rose of toiletry bag % containing weight of the invention meter
It is rare fruit oil 0.5-2.5, guava extract 2.0-4.0, card slave's card leaf extract 4.0-6.0,1,3 propane diols 2.0-4.0, transparent
Matter acid sodium 4.0-6.0, Rosa Damascana 1.5-3.5, soybean lecithin 2.0-4.0, resveratrol 5.0-12.0, carbomer 0.4-
0.8th, glucan 2.0-4.0, niacinamide 1.0-2.0 and dried tremella extract 2.0-4.0.
It is highly preferred that toiletry bag weight containing the 0.0005-0.001 % of present invention SEQ ID NO：1 peptide and/or SEQ
ID NO：2 peptides.It is further preferred that the cosmetics of the present invention also the lauroyl arginine ethyl ester comprising appropriate amount and the water of surplus.
It is further preferred that the cosmetics with regulation sebum secretion effect of the present invention, it is included：SEQ ID NO：1
With SEQ ID NO：One or both of 2 peptide, retinol, capsule of weeping forsythia berry extract, Salvia root P.E, anemarrhena asphodefoides extract, white willow
Bark extract, 1,3 propane diols, shrubby sophora extract, soybean lecithin, glucan, niacinamide and lauroyl arginine ethyl ester.
It is further preferred that cosmetics of the present invention, it is included with weight %：
Retinol 2.0-4.0, capsule of weeping forsythia berry extract 0.5-2.5, Salvia root P.E 0.5-2.5, anemarrhena asphodefoides extract 2.0-
4.0th, white willow bark extract 4.0-6.0,1,3 propane diols 2.0-4.0, shrubby sophora extract 4.0-6.0, soybean lecithin 2.0-
4.0th, glucan 2.0-4.0, niacinamide 1.0-2.0 and lauroyl arginine ethyl ester 2.0-4.0.
As it is used herein, "comprising" and " comprising ", " containing " or " being characterised by " it is synonymous, and in being included in
Or open, and it is not excluded for the other element do not stated or method and step.Term "comprising" any table herein
State, particularly in the method for the description present invention, purposes or during product, it is thus understood that including substantially by the component or element or
Those products, method and purposes that step is constituted and is made up of the component or element or step.The sheet of description exemplified here
Invention suitably can be in the situation in the absence of any one or more of element not specifically disclosed herein, one or more limitations
It is lower to be put into practice.
The specification for being related to the regulation sebum secretion product included in the regulation sebum secretion product of the present invention can be with
Contain following content：Effect (is for example uniformly applied to skin, such as face with effect (such as adjusting sebum secretion), application process
Portion and neck) and issuable side effect etc..
The term and statement used herein is used as descriptively rather than restrictive term, and in such a term and statement
Use in be not expected to exclude shown in and the feature or part thereof any equivalent, it is appreciated that various modifications are in request
It is possible in the scope of the present invention of protection.Although it is therefore understood that the present invention by preferred embodiment and optionally
Feature is specifically disclosed, but those skilled in the art can use the modification and transformation of concept disclosed herein, and such modification
It is considered as with change such as in the scope of the present invention that accessory claim is defined.
To be illustrated more clearly that the present invention, it is described in detail in conjunction with following examples, but these embodiments are only
To the exemplary description of the present invention, the limitation to the application should not be construed as.
Embodiment
The following examples are to be used to illustrate the present invention in a non limiting manner.
Embodiment 1
Peptide of the present invention suppresses the experiment of sebum secretion
1st, people's sebaceous glands cell separation and original cuiture
Normal person's skin of head fresh specimens use PBS, 70% alcohol washes 1 time successively, are placed inNutrient solution
In (plus 2% hyclone, antibiotic cocktail).Subcutaneous fat is removed, skin is cut into 0.5cm × 0.5cm sizes, plus it is neutral
4 DEG C of fridge overnights are placed in after protease, sealing.Epidermis is peeled off under stereomicroscope, more than 15 sebums are separated with tweezers
Gland, is placed in the Tissue Culture Dish with collagenic coating (in the Tissue Culture Dish of 6cm diametersNutrient solution is no more than
In 1.5mL), it is placed in 5%CO2 cell culture incubators, 37 DEG C of 2~3d of quiescent culture.After sebocyte cell is fixed, changeNutrient solution (every bottle of nutrient solution adds 10% hyclone, 5 μ g/L recombined humans EGF, antibiotic mixture), is placed in
37 DEG C of cultures in 5%CO2 cell culture incubators, 2~3d changes a nutrient solution, passed on after cell is covered with.
2nd, peptide processing sebocyte cell
When sebocyte cell culture is to 70%, respectively with the SEQ ID NO of various concentration：1 or SEQ ID NO：At 2 peptide
Cell is managed, untreated cell continues to cultivate 24h as blank control group.
3rd, lipid content reduces the measure of ratio
Cell is collected, PBS is rinsed, distilled water flushing, 0.5% oil red O stain 5min, PBS flushing, 200uL isopropyl alcohol leaching
5min extracting oil red O are steeped, oil red O aqueous isopropanol is obtained, OD values are determined in 510nm wavelength with spectrophotometer, according to mark
Directrix curve is scaled standard value, then using blank control group as control, calculates the reduction ratio of each group lipid content.As a result it is shown in
Table 1 below.
Table 1：Lipid content reduces ratio
From the data of table 1 can be seen that two kinds of peptides of the present invention between cooperative interaction thus assign the sebum point of reinforcement
Secrete inhibition.
Embodiment 2：The preparation of blank cosmetics
Embodiment 3：The preparation of cosmetics
| Composition | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：1) | 0.001 |
| Retinol | 3.0 |
| Capsule of weeping forsythia berry extract | 1.5 |
| Salvia root P.E | 1.5 |
| Anemarrhena asphodefoides extract | 3.0 |
| White willow bark extract | 5.0 |
| 1,3 propane diols | 3.0 |
| Shrubby sophora extract | 5.0 |
| Soybean lecithin | 3.0 |
| Glucan | 3.0 |
| Niacinamide | 1.0 |
| Lauroyl arginine ethyl ester | 3.0 |
| Purified water | Residual volume |
| Amount to | 100 |
Glucan is dissolved in purified water and 1,3 propane diols are added, is stirring evenly and then adding into after other components and is stirred and adjust
Cosmetic cream is made.
Embodiment 4：The preparation of cosmetics
Glucan is dissolved in purified water and 1,3 propane diols are added, is stirring evenly and then adding into after other components and is stirred and adjust
Cosmetic cream is made.
Embodiment 5：The preparation of cosmetics
| Composition | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：1) | 0.0005 |
| Peptide (the SEQ ID NO of the present invention：2) | 0.0005 |
| Retinol | 4.0 |
| Capsule of weeping forsythia berry extract | 2.5 |
| Salvia root P.E | 2.5 |
| Anemarrhena asphodefoides extract | 4.0 |
| White willow bark extract | 6.0 |
| 1,3 propane diols | 4.0 |
| Shrubby sophora extract | 6.0 |
| Soybean lecithin | 4.0 |
| Glucan | 4.0 |
| Niacinamide | 1.5 |
| Lauroyl arginine ethyl ester | 2.0 |
| Purified water | Residual volume |
| Amount to | 100 |
Glucan is dissolved in purified water and 1,3 propane diols are added, is stirring evenly and then adding into after other components and is stirred and adjust
Cosmetic cream is made.
Embodiment 6
According to the formula shown in embodiment 2,3,4 and 5, the composition for external application (cosmetics) of emulsifiable paste shape is modulated, wherein
The blank cosmetics of embodiment 2 embodiment as a comparison.
Subject is randomly divided into 4 groups, every group 10, group numbering is designated as #1-#4.Subject is in 22 ± 3 DEG C of temperature, phase
To being sat quietly 30 minutes in the laboratory of humidity 50 ± 5%, left and right forehead is tested respectively with grease tester Sebumeter SM815
Skin oil and fat content, be designated as D0 data, blank sample #1 and each group reference numeral sample be distributed to each subject, and
Instruct subject to carry out the contrast of half face to try out, two kinds of samples are smeared respectively and are used in left half face or right half face, it is once each to smear
Sample 0.5g, once in the morning and once at night, continues 28 days, and returns constant temperature laboratory test skin oil at the 14th day and the 28th day respectively
Fat content, data are designated as D14 and D28.Calculate D14 and D28 fat content rate of change respectively as follows：
Fat content rate of change=(using fat content before fat content-use sample after sample)/using oil before sample
Fat content } × 100%
As a result it is as shown in table 2.
Table 5
| Test sample | D14 greases rate of change (%) | D28 greases rate of change (%) |
| Sample #1 | - 8.5% | - 11.6% |
| Sample #2 | - 28.2% | - 35.8% |
| Sample #3 | - 19.3% | - 25.6% |
| Sample #4 | - 46.6% | - 59.8% |
As a result show, use the reality that with the addition of the composition prepared in embodiment 3 in the present invention, embodiment 4 or embodiment 5
The subject of sample is tested, at 14 days, skin oil and fat content was decreased obviously, persistently using after 28 days, effect is more obvious, and its
The middle subject for having used the laboratory sample of the composition of embodiment 5 containing two kinds of peptides simultaneously, skin oil and fat content declines the most
Substantially, illustrate the effect that Synergistic has been reached after two kinds of peptide combinations, use the tested of the laboratory sample prepared containing embodiment 2
Person, oil-control effect is substantially not so good as other samples, but due to also containing oil-control composition in other compositions, so also having certain reduction
The effect of skin oil and fat.
Embodiment 7
Anti-acne effect is evaluated
Evaluation object：The young man 40 that selection face has acne, the age is 15-30 Sui, wherein male 20, women 20
Example.
Evaluation method：Using double-blind study, after every morning washed, the cosmetics 1g of Example 5 is applied to acne on the face
Position, applied again once after washed before evening sleep, be within 20 days a course for the treatment of.
Evaluation criterion：1. it is effective：Acne fading more than 70%；2. it is effective：Acne fading more than 30%；3. it is invalid：Acne
Below fading 30%.
Evaluation result：Effective 22, account for 55%；Effective 13, account for 32.5%；Invalid 5, account for 12.5%.Total effective rate
87.5% is reached, illustrates that the present composition has good anti-acne effect.
By the description of above-mentioned preferred embodiment, the present invention is described it should be appreciated that skill
Art personnel can make a change and change to these embodiments, without departing from of the invention as expressed by following claims
Spirit or scope.
Sequence table
<110>The auspicious bio tech ltd in Wenzhou thousand
<120>Adjust the peptide of sebum secretion and the cosmetics comprising it
<130> 2
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 10
<212> PRT
<213>Artificial sequence
<220>
<223>Adjust the peptide of sebum secretion
<400> 1
Ser Val Ala Lys Arg Glu Cys Arg Asp Gln
1 5 10
<210> 2
<211> 9
<212> PRT
<213>Artificial sequence
<220>
<223>Adjust the peptide of sebum secretion
<400> 2
Glu His Gly Pro Phe Asp Ala Gln Pro
1 5
Claims (10)
1. peptide, it is referred to as " peptides of Peptide 07 ", with SEQ ID NO：1 amino acid sequence.
2. peptide according to claim 1, it is by SEQ ID NO：1 amino acid sequence composition.
3. the combination of peptide, it includes SEQ ID NO：1 peptides of Peptide 07 and SEQ ID NO：2 peptide.
4. for adjusting the composition of sebum secretion, it includes the peptide of claim 1 or 2, or the peptide comprising claim 3 group
Close.
5. the peptide of claim 1 or 2 is preparing the purposes in being used to adjust the composition of sebum secretion.
6. purposes according to claim 5, wherein the composition is the form of cosmetics.
7. the cosmetics with regulation sebum secretion effect, it is included：SEQ ID NO：1 and SEQ ID NO：One of 2 peptide or
Both, retinol, capsule of weeping forsythia berry extract, Salvia root P.E, anemarrhena asphodefoides extract, white willow bark extract, 1,3 propane diols, kuh-seng
Extract, soybean lecithin, glucan, niacinamide and lauroyl arginine ethyl ester.
8. cosmetics according to claim 7, it is included with weight %：
It is retinol 2.0-4.0, capsule of weeping forsythia berry extract 0.5-2.5, Salvia root P.E 0.5-2.5, anemarrhena asphodefoides extract 2.0-4.0, white
Extract of willow bark 4.0-6.0,1,3 propane diols 2.0-4.0, shrubby sophora extract 4.0-6.0, soybean lecithin 2.0-4.0, Portugal gather
Sugared 2.0-4.0, niacinamide 1.0-2.0 and lauroyl arginine ethyl ester 2.0-4.0.
9. cosmetics according to claim 7, wherein the cosmetics are packaged as commercially available cosmetics.
10. cosmetics according to claim 9, wherein described cosmetics also include the operation instructions of product.
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| CN201710665862.XA CN107325157B (en) | 2017-08-07 | 2017-08-07 | Sebum secretion-regulating peptide and cosmetic containing same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022051831A1 (en) | 2020-09-11 | 2022-03-17 | Chemyunion Ltda. | Composition, use of the composition, cosmetic method and method for modulating the production of sebum |
| CN114515264A (en) * | 2022-03-04 | 2022-05-20 | 广州品域美妆创新科技有限公司 | Composition for regulating excessive sebum secretion, preparation method thereof and cosmetic |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012130775A1 (en) * | 2011-03-25 | 2012-10-04 | Lipotec S.A. | Pgc-1alpha-modulating peptides |
| CN105560128A (en) * | 2016-03-01 | 2016-05-11 | 广东轻工职业技术学院 | Composition with efficacy of inhibiting sebum secretion and use of composition |
| WO2016191864A1 (en) * | 2015-05-29 | 2016-12-08 | Changiz Taghibiglou | Polypeptides and antibodies to treat skin conditions associated with overproduction of sebum |
-
2017
- 2017-08-07 CN CN201710665862.XA patent/CN107325157B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012130775A1 (en) * | 2011-03-25 | 2012-10-04 | Lipotec S.A. | Pgc-1alpha-modulating peptides |
| WO2016191864A1 (en) * | 2015-05-29 | 2016-12-08 | Changiz Taghibiglou | Polypeptides and antibodies to treat skin conditions associated with overproduction of sebum |
| CN105560128A (en) * | 2016-03-01 | 2016-05-11 | 广东轻工职业技术学院 | Composition with efficacy of inhibiting sebum secretion and use of composition |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022051831A1 (en) | 2020-09-11 | 2022-03-17 | Chemyunion Ltda. | Composition, use of the composition, cosmetic method and method for modulating the production of sebum |
| CN114515264A (en) * | 2022-03-04 | 2022-05-20 | 广州品域美妆创新科技有限公司 | Composition for regulating excessive sebum secretion, preparation method thereof and cosmetic |
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| Publication number | Publication date |
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| CN107325157B (en) | 2020-06-26 |
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