CN107325157B - Sebum secretion-regulating peptide and cosmetic containing same - Google Patents

Abstract

The present invention relates to an active peptide having an effect of regulating sebum secretion and a cosmetic comprising the same. Specifically, the invention discloses SEQ ID NO 1 and SEQ ID NO:2, alone or in combination, for sebum secretion regulating activity and a cosmetic comprising the peptide, wherein the cosmetic comprises in weight%: 2.0-4.0 parts of retinol, 0.5-2.5 parts of forsythia fruit extract, 0.5-2.5 parts of salvia extract, 2.0-4.0 parts of rhizoma anemarrhenae extract, 4.0-6.0 parts of white willow bark extract, 2.0-4.0 parts of 1,3 propylene glycol, 4.0-6.0 parts of radix sophorae flavescentis extract, 2.0-4.0 parts of soybean lecithin, 2.0-4.0 parts of glucan, 1.0-2.0 parts of nicotinamide and 2.0-4.0 parts of lauroyl arginine ethyl ester. The cosmetic has the effects of inhibiting sebum secretion, treating acne and the like.

Description

Sebum secretion-regulating peptide and cosmetic containing same

Technical Field

The present invention relates to non-natural peptides having a sebum secretion regulating effect and their use in cosmetic and personal care compositions.

Background

Normal skin contains accessory structures such as hair follicles, sebaceous glands, sweat glands, and the like. Sebaceous glands (seboeusgland) are important accessory structures in the skin, mostly located between hair follicles and pileus muscle, and are vesicular glands consisting of one or several vesicular acini and a common short duct; the duct is a stratified squamous epithelium with multiple openings at the upper section of the hair follicle and some openings directly at the skin surface. The sebaceous glands are distributed over the entire area except the skin of the palms and soles. The sebaceous gland secretion is sebum, is a mixture of 1, 3-propylene glycol triester, fatty acid, wax ester, sterol ester, etc., and has effects of moistening skin, reducing water loss of skin, and regulating natural microbial composition of skin.

There are two kinds of sebaceous glands, one is a sebaceous gland that is connected to a hair follicle in the pilosebaceous gland, and the other is an independently present sebaceous gland. Sebaceous glands are also present in the hairless areas of the eyelids, nose, penis, labia minora, inner mucosa of the cheek and papillae.

The amount of sebum secreted by sebaceous glands is regulated by the level of androgens, the nutritional status and mood of the human body. When these factors are disordered and pores on the skin are blocked by dirt, and hormone secretion disorder is added to cause follicular keratosis, so that the blockage of the follicles is further aggravated, acne formation is caused, and when bacteria multiply in the accumulated sebum to cause inflammation, acne (commonly called whelk) is formed to cause skin damage.

Excessive sebum secretion from the sebaceous glands of the scalp can lead to oily hair and even to the production of dandruff. Dandruff is a disorder of the scalp characterized by a rich, loose patch in the form of a sheet, usually accompanied by itching. An aggravated desquamation of the scalp can progress to seborrheic dermatitis, a severe dandruff with inflammation and erythema.

U.S. patent 6,488,318 discloses methods and compositions for reducing sebum secretion using 1,2,3, 4-tetrahydro-1, 1,4, 4-tetramethyl-6- [ α -methylstyryl ] naphthalene as an active ingredient and for preventing or treating diseases caused by excessive sebum secretion, such as acne or oily scalp and hair.

Us patent 11,991,587 discloses the cosmetic use of at least one protoberberine/procyanidin other than berberine, or a plant extract containing it, applied to the skin as an active substance to inhibit the activity of the sebaceous unit. Also disclosed is a cosmetic composition containing the same.

Us patent 8,298,735 discloses a method of treating a disease caused by sebaceous gland disorders in humans and animals comprising administering to said humans and animals a composition comprising a sebaceous gland secretion inhibiting amount of an active compound comprising an acyl-coa cholesterol acyltransferase (ACAT) inhibitor or a prodrug thereof.

Chinese patent CN105560128 discloses a composition with effect of inhibiting sebum secretion and application thereof. The composition is prepared from the following components: shale oil sodium sulfonate, water-soluble tea tree oil, a traditional Chinese medicine composition and deionized water; wherein the Chinese medicinal composition is prepared from flos Chrysanthemi Indici, pericarpium Granati, flos Lonicerae, radix asparagi, and radix et caulis Opuntiae Dillenii. The composition can effectively reduce skin oil secretion, can control skin oil secretion for a long time after being continuously used, is safe and non-irritant to skin, is used in cosmetics for controlling oil and removing acnes, and has the effects of controlling oil and removing acnes of Liangha.

Chinese patent CN103458908 discloses a composition comprising guava fruit extract or kunzea ericoides leaf extract or both for reducing sebum production in sebaceous glands, which can reduce the amount of sebum produced by sebaceous glands, open pores and treat acne.

The problems of acne, oily hair, dandruff and seborrheic dermatitis, although not serious health problems, can cause great adverse effects on personal image, serious psychological burden on patients and serious influence on work and life of patients, so that the solution of the problems of acne, oily hair, dandruff and seborrheic dermatitis is of great significance to personal health and social development. At present, medicines or cosmetics containing antibiotics or hormones are often used for treatment, but the medicines also often bring irritation and adverse effects to the skin while treatment, rebound often occurs after short-term treatment withdrawal, and skin chap and even drug resistance are generated after long-term use. Therefore, it is desired to solve the problems of acne, oily hair, dandruff, seborrheic dermatitis, etc. by balancing sebum secretion and constructing a skin health microenvironment.

Brief description of the invention

The present inventors have conducted intensive studies and screened peptide libraries in order to develop novel peptides having the effect of regulating sebum secretion. Finally, the present inventors have found a novel peptide having excellent efficacy of regulating sebum secretion, and finally achieved the object of the present invention.

An object of the present invention is to provide a peptide having an effect of regulating sebum secretion.

It is another object of the present invention to provide a composition for regulating sebum secretion.

It is a further object of the present invention to provide a method for modulating sebum secretion.

To achieve the object of the present invention, in a first aspect of the present invention, there is provided a peptide selected from the group consisting of:

(i) a peptide comprising or consisting of amino acid residues as follows:

Ser-Val-Ala-Lys-Arg-Glu-Cys-Arg-Asp-Gln (SVAKRECRDQ) (SEQ ID NO:1, also known as "Peptide 07"); or

(ii) A peptide comprising or consisting of amino acid residues as follows:

Glu-His-Gly-Pro-Phe-Asp-Ala-Gln-Pro(EHGPFDAQP)(SEQ ID NO:2)。

according to a preferred aspect of the present invention, there is provided a combination of peptides comprising the peptide combination of SEQ ID NO. 1 and SEQ ID NO. 2.

According to a second aspect of the present invention, there is provided a sebum secretion-regulating composition comprising the peptide of SEQ id No. 1 of the present invention and a cosmetically acceptable carrier.

According to a preferred aspect of the present invention, there is provided a sebum secretion-regulating composition comprising the amino acid sequence of SEQ ID No. 1 and SEQ ID NO:2 and a cosmetically acceptable carrier.

According to a third aspect of the present invention, there is provided the use of a peptide of SEQ ID NO. 1 of the present invention for modulating sebum secretion.

According to a preferred aspect of the present invention, there is provided SEQ ID NO 1 and SEQ ID NO:2 for use in modulating sebum secretion.

The present invention also provides a cosmetic for regulating sebum secretion, comprising:

(a) individual peptides or combinations of peptides of the invention;

(b) an antioxidant;

(C) a humectant;

(d) a vegetable oil;

(e) lecithin;

(f) a preservative;

(g) essential oil; and

(h) and (5) purifying the water.

These and other aspects of the present invention will become apparent to those skilled in the art upon reading the following detailed description of the invention, including the exemplary embodiments and examples.

Detailed Description

Two peptides are claimed in the present invention, namely peptide (i) having SEQ ID NO:1 or peptide (ii) having SEQ ID NO: 2. When these peptides are used in a sebum secretion modulating composition, the inhibition provides benefits in modulating sebum secretion.

In addition, by experimentation, it was not only demonstrated that each of these two peptides reduces sebum secretion, but also that they interact synergistically when used together.

As used herein, all terms are intended to have their ordinary meaning in the art unless otherwise specifically defined. The term "amino acid" is intended to include naturally occurring amino acids as well as non-naturally occurring amino acids, and broadly includes all small molecules having one carboxyl group and at least one primary or secondary amine group capable of forming a peptide bond. The term "peptide" is intended to include all molecules having at least one peptide bond, and thus includes dipeptides, tripeptides, oligopeptides and polypeptides having up to about 20 amino acid residues. The term "peptide" also includes structures other than amino acids having one or more linkers, spacers, or terminal groups.

The peptides of the invention are provided in a cosmetically acceptable carrier. The carrier may be hydrophobic or hydrophilic. Suitable hydrophobic carriers include, for example, waxy nonionic materials commonly used in cosmetics, such as esters and ethers of fatty alcohols and fatty acids, with carbon chain lengths of C4 to C22, preferably C8 to C18, most preferably C12 to C18.

Examples of fatty hydrophobic carriers include isopropyl myristate, isopropyl palmitate, octyl palmitate, isopropyl lanolate, acetylated lanolin alcohol, benzoate esters of C12-C15 alcohols, cetyl octanoate, cetyl palmitate, myristyl myristate, myristyl lactate, cetyl acetate, propylene glycol dicaprylate/decanoate, decyl oleate, acetylated lanolin, heptanoic stearate, diisostearate malate, octyl hydroxystearate, isopropyl isostearate, and the like.

Suitable hydrophilic carrier solutions may be, for example, glycols and alkoxylated glycols commonly used in cosmetics, including ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol, and the like.

The sebum secretion-regulating composition may be formulated as a nutritional liquid, essence, cosmetic, cream, lotion, serum, spray, stick, and other forms known to those skilled in the art. Creams and lotions are currently the preferred product forms.

In a cosmetically acceptable carrier, the concentration of the peptide may range from 1. mu.M to 200. mu.M, preferably from 10ppb to 150. mu.M, more preferably from 20. mu.M to 100. mu.M, most preferably from 36. mu.M to 50. mu.M.

Sebum secretion-regulating compositions typically comprise a carrier solution as described above at a level of from about 0.01% to about 90% by weight, preferably from about 0.1% to about 50% by weight, more preferably from about 0.1% to about 20% by weight, most preferably from about 1% to about 10% by weight.

Examples of the antioxidant include butylhydroxyanisole, dibutylhydroxytoluene, sodium hydrogensulfite, erythorbic acid and salts thereof, glutathione peroxidase, glutathione-S-transferase, catalase, superoxide dismutase, thioredoxin, taurine, thiotaurine, hypotaurine, and vitamin E. Among them, glutathione, vitamin E and catalase are preferable. These antioxidants may be used in combination of 1 or more than 2, and are preferably a combination of glutathione and vitamin E. When the antioxidant is added to the composition for external application to the skin (for example, a cosmetic) of the present invention, the addition ratio thereof is not particularly limited, but is usually 0.001 to 6% by weight, preferably 0.01 to 3% by weight, and more preferably 0.2 to 2% by weight, based on the total amount of the composition for external application to the skin.

Examples of the humectant include amino acids such as alanine, serine, leucine, isoleucine, threonine, glycine, proline, hydroxyproline, aspartic acid, arginine, and theanine, and derivatives thereof; polyhydric alcohols such as ethylene glycol, 1, 3-propanediol, 1, 3-butanediol, diethylene glycol, triethylene glycol, tetraethylene glycol, dipropylene glycol, di-1, 3-propanediol, and polyethylene glycol; glycol ethers such as ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, diethylene glycol monobutyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, dipropylene glycol monoethyl ether, and dipropylene glycol monopropyl ether; sugar alcohols such as mannitol, sorbitol, erythritol, xylitol, and trehalose; phospholipids such as lecithin and hydrogenated lecithin; mucopolysaccharides such as sodium hyaluronate, sodium acetylhyaluronate, heparin analogues, sodium chondroitin sulfate, etc.; high molecular compounds such as collagen, elastin, keratin, chitin, chitosan, gelatin, polyglutamic acid, etc.; sodium lactate, sodium pyrrolidone carboxylate, etc.; chamomile extract, aloe vera extract, witch hazel extract, rosemary extract, thyme extract, tea extract, perilla extract, and other plant extracts. Among them, glycine, 1, 3-propanediol, dipropylene glycol, di-1, 3-propanediol, diethylene glycol monoethyl ether, sodium hyaluronate, sodium acetylhyaluronate, sodium pyrrolidone carboxylate, and polyglutamic acid are preferable. These moisturizers may be used in 1 kind or in combination of 2 or more kinds, and 1, 3-propanediol and sodium hyaluronate are preferred. When the moisturizing agent is added to the composition for external application to the skin (e.g., cosmetic) of the present invention, the addition ratio is not particularly limited, but is usually 0.001 to 40% by weight, preferably 0.1 to 30% by weight, and more preferably 5 to 20% by weight, based on the total amount of the composition for external application to the skin (e.g., cosmetic).

The vegetable oil is selected from rose hip oil, sweet almond oil, rose hip oil, jojoba oil, wheat germ oil, carrot oil, avocado oil, castor oil, avocado oil, coconut oil, palm oil, cocoa butter, palm kernel oil, soybean oil, rice bran oil, aloe oil, veronica officinalis, chenopodium album butter, shea butter, peanut oil, corn oil, macadamia nut oil, Chinese chestnut oil, seabuckthorn oil, linseed oil, passion flower oil, calendula, mango kernel oil and rose hip oil, and is preferably one or a combination of two or more of sweet almond oil, jojoba oil and aloe oil. Preferably, the vegetable oil in the composition of the invention is rose hip oil. When the vegetable oil is added to the composition for external application to the skin (e.g., cosmetic) of the present invention, the addition ratio thereof is not particularly limited, but is usually 0.001 to 10% by weight, preferably 0.5 to 8% by weight, and more preferably 1.0 to 8.0% by weight, based on the total amount of the composition for external application to the skin (e.g., cosmetic).

As preservative, it is selected from benzyl alcohol, phenethyl alcohol, phenoxyethanol and lauroyl arginine ethyl ester, preferably lauroyl arginine ethyl ester. When a preservative is added to the composition for external application to the skin (e.g., cosmetic) of the present invention, the addition ratio is not particularly limited, but is usually 0.001 to 5% by weight, preferably 0.1 to 5% by weight, and more preferably 0.2 to 3% by weight, based on the total amount of the composition for external application to the skin (e.g., cosmetic).

As the lecithin, it is preferably soybean lecithin. When lecithin is added to the composition for external application to skin (e.g., a cosmetic) of the present invention, the addition ratio is not particularly limited, but is usually 0.01 to 5% by weight, preferably 0.1 to 5% by weight, and more preferably 0.2 to 2% by weight, based on the total amount of the composition for external application to skin (e.g., a cosmetic).

Preferably, the cosmetic comprises 2.0-4.0% of glutathione, 0.5-2.5% of vitamin E, 0.5-2.5% of rose hip oil, 2.0-4.0% of guava extract, 4.0-6.0% of Kunzea leaf extract, 2.0-4.0% of 1, 3-propylene glycol, 4.0-6.0% of sodium hyaluronate, 1.5-3.5% of rose essential oil, 2.0-4.0% of soybean lecithin, 5.0-12.0% of resveratrol, 0.4-0.8% of carbomer, 2.0-4.0% of glucan, 1.0-2.0% of nicotinamide and 2.0-4.0% of tremella extract in percentage by weight.

More preferably, the cosmetic of the invention comprises 0.0005 to 0.001% by weight of the amino acid sequence of SEQ ID NO:1 peptide and/or seq id NO:2 peptides. Also preferably, the cosmetic of the present invention further comprises a suitable amount of ethyl lauroyl arginate and the balance of water.

Further preferably, the cosmetic with sebum secretion regulating effect of the present invention comprises: SEQ ID NO:1 and SEQ ID NO:2, retinol, forsythia suspensa fruit extract, salvia miltiorrhiza extract, anemarrhena asphodeloides extract, willow bark extract, 1,3 propylene glycol, sophora flavescens extract, soybean lecithin, dextran, nicotinamide and lauroyl arginine ethyl ester.

Still more preferably, the cosmetic according to the present invention comprises, in% by weight:

2.0-4.0 parts of retinol, 0.5-2.5 parts of forsythia fruit extract, 0.5-2.5 parts of salvia extract, 2.0-4.0 parts of rhizoma anemarrhenae extract, 4.0-6.0 parts of white willow bark extract, 2.0-4.0 parts of 1,3 propylene glycol, 4.0-6.0 parts of radix sophorae flavescentis extract, 2.0-4.0 parts of soybean lecithin, 2.0-4.0 parts of glucan, 1.0-2.0 parts of nicotinamide and 2.0-4.0 parts of lauroyl arginine ethyl ester.

As used herein, "comprising" is synonymous with "including," "containing," or "characterized by," and is inclusive or open-ended and does not exclude additional unrecited elements or method steps. The term "comprising" in any of the expressions herein, particularly in describing the method, use or product of the invention, is to be understood as including those products, methods and uses which consist essentially of and consist of the recited components or elements or steps. The invention illustratively described herein suitably may be practiced in the absence of any element or elements, limitation or limitations which is not specifically disclosed herein.

The instructions contained in the sebum secretion regulating product of the present invention relating to the sebum secretion regulating product may contain the following contents: effects and efficacies (e.g. regulation of sebum secretion), methods of application (e.g. even application to the skin, e.g. face and neck), and possible side effects, etc.

The terms and expressions which have been employed herein are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.

For a more clear illustration of the invention, reference is now made in detail to the following examples, which are intended to be purely exemplary of the invention and are not to be interpreted as limiting the application.

Examples

The following examples are intended to illustrate the invention in a non-limiting manner.

Example 1

Test for inhibition of sebum secretion by the peptide of the present invention

1. Isolation and primary culture of human sebaceous gland cells

Washing fresh skin specimen of normal human head with PBS and 70% alcohol for 1 time, and placing in

Removing subcutaneous fat, cutting skin into size of 0.5cm × 0.5.5 cm, adding neutral protease, sealing, placing in refrigerator at 4 deg.C overnight, peeling off epidermal layer under stereomicroscope, separating more than 15 sebaceous glands with tweezers, and placing in cell culture dish with collagen coating (6cm diameter)

Culture solution is not more than 1.5mL), and the mixture is placed in a 5% CO2 cell incubator and is subjected to static culture at 37 ℃ for 2-3 d. After the sebaceous gland cells are fixed, the cells are replaced

And (3) culturing the culture solution (each bottle of culture solution is added with 10% fetal calf serum, 5 mu g/L recombinant human EGF and an antibiotic mixture) at 37 ℃ in a 5% CO2 cell culture box, replacing the culture solution for 2-3 days, and carrying out passage after the cells grow full.

2. Peptide treatment of sebaceous gland cells

When sebaceous gland cells were cultured to 70%, the cells were cultured with various concentrations of SEQ ID NO:1 or SEQ ID NO:2 and untreated cells as a blank, and incubation was continued for 24 h.

3. Determination of lipid content reduction ratio

Collecting cells, washing with PBS, washing with distilled water, staining with 0.5% oil red O for 5min, washing with PBS, soaking in 200uL isopropanol for 5min to extract oil red O, obtaining isopropanol solution of oil red O, measuring OD value at 510nm wavelength with spectrophotometer, converting into standard value according to standard curve, and calculating the reduction ratio of lipid content in each group with blank control group as control. The results are shown in Table 1 below.

Table 1: lipid content reduction ratio

As can be seen from the data of table 1, a synergistic interaction between the two peptides of the present invention thus imparts an enhanced sebum secretion inhibiting effect.

Example 2: preparation of blank cosmetic

Example 3: preparation of cosmetics

Composition (I)	Content (wt%)
		The peptides of the present invention (SEQ ID NO: 1)	0.001
Retinol	3.0
		Forsythia fruit extract	1.5
Salvia miltiorrhiza extract	1.5
		Rhizoma anemarrhenae extract	3.0
White willow bark extract	5.0
		1,3 propanediol	3.0
Sophora flavescens extract	5.0
		Soybean lecithin	3.0
Glucan	3.0
		Nicotinamide	1.0
Lauroyl arginine ethyl ester	3.0
		Purified water	Residual amount
Total of	100

Dissolving dextran in purified water, adding 1, 3-propylene glycol, stirring, adding other components, stirring, and making into cosmetic cream.

Example 4: preparation of cosmetics

Dissolving dextran in purified water, adding 1, 3-propylene glycol, stirring, adding other components, stirring, and making into cosmetic cream.

Example 5: preparation of cosmetics

Composition (I)	Content (wt%)
		The peptides of the present invention (SEQ ID NO: 1)	0.0005
The peptides of the present invention (SEQ ID NO: 2)	0.0005
		Retinol	4.0
Forsythia fruit extract	2.5
		Salvia miltiorrhiza extract	2.5
Rhizoma anemarrhenae extract	4.0
		White willow bark extract	6.0
1,3 propanediol	4.0
		Sophora flavescens extract	6.0
Soybean lecithin	4.0
		Glucan	4.0
Nicotinamide	1.5
		Lauroyl arginine ethyl ester	2.0
Purified water	Residual amount
		Total of	100

Dissolving dextran in purified water, adding 1, 3-propylene glycol, stirring, adding other components, stirring, and making into cosmetic cream.

Example 6

Cream-like skin external compositions (cosmetics) were prepared according to the formulations shown in examples 2,3,4 and 5, with the blank cosmetic of example 2 as a comparative example.

The subjects were randomized into 4 groups of 10, with group numbers #1- # 4. The test subjects sit still for 30 minutes in a laboratory with the temperature of 22 +/-3 ℃ and the relative humidity of 50 +/-5 percent, the skin oil content of the left forehead and the right forehead is respectively tested by using an oil tester Sebumeter SM815 and the data is recorded as D0, blank samples #1 and samples with corresponding numbers of each group are distributed to each test subject, the test subjects are guided to carry out half-face comparison test, the two samples are respectively smeared on the left half face or the right half face, 0.5g of the sample is smeared once in the morning and at the evening every day and lasts for 28 days, and the skin oil content is respectively tested in a constant temperature laboratory on the 14 th day and the 28 th day and the data is recorded as D14 and D28. Respectively calculating the oil content change rate of D14 and D28 according to the following formula:

oil content change rate { (oil content after use sample-oil content before use sample)/oil content before use sample } × 100%

The results are shown in Table 2.

TABLE 5

Test sample	D14 change in fat (%)	D28 change in fat (%)
			Sample #1	-8.5％	-11.6％
Sample #2	-28.2％	-35.8％
			Sample #3	-19.3％	-25.6％
Sample #4	-46.6％	-59.8％

The results show that the subjects who used the composition prepared in example 3, example 4 or example 5 of the present invention had a significant decrease in skin oil content at 14 days, and the effect was more significant after the composition was continuously used for 28 days, while the subjects who used the composition prepared in example 5 containing two peptides had the most significant decrease in skin oil content, indicating that the two peptides had a synergistic effect in combination, and the subjects who used the composition prepared in example 2 had a significantly lower oil control effect than the other samples, but had a certain effect of reducing skin oil because the other components contained oil control components.

Example 7

Evaluation of acne treatment Effect

Evaluation target: 40 young people with acne on their face and 15-30 years of age were selected, 20 male and 20 female.

The evaluation method comprises the following steps: by adopting a double-blind method, 1g of the cosmetic of example 5 is applied to a part with acne on the face after the face is washed in the morning, and the cosmetic is applied once after the face is washed before sleep at night, wherein 20 days is a treatment course.

The evaluation standard is that ① has obvious effect that acne is faded by more than 70 percent, ② has effective effect that acne is faded by more than 30 percent, ③ has no effect that acne is faded by less than 30 percent.

Evaluation results were as follows: 22 cases of obvious effect account for 55 percent; 13 cases of the drug are effective, accounting for 32.5 percent; and 5 invalid cases account for 12.5 percent. The total effective rate reaches 87.5 percent, which shows that the composition has good acne removing effect.

The present invention has been illustrated by the description of the preferred embodiments above, but it is to be understood that variations and modifications can be made on these embodiments by the skilled person without departing from the spirit or scope of the present invention as expressed in the following claims.

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Claims (9)

1. A Peptide, referred to as "Peptide 07", consisting of SEQ ID NO: 1.

2. A combination of peptides comprising SEQ ID NO:1 and the Peptide of SEQ ID NO: 2.

3. A composition for modulating sebum secretion, comprising the peptide of claim 1, or a combination comprising the peptide of claim 2.

4. Use of a peptide according to claim 1 for the preparation of a composition for modulating sebum secretion.

5. Use according to claim 4, wherein the composition is in the form of a cosmetic product.

6. A cosmetic having an effect of regulating sebum secretion, comprising: SEQ ID NO:1 and SEQ ID NO:2, retinol, forsythia suspensa fruit extract, salvia miltiorrhiza extract, anemarrhena asphodeloides extract, willow bark extract, 1,3 propylene glycol, sophora flavescens extract, soybean lecithin, dextran, nicotinamide and lauroyl arginine ethyl ester.

7. The cosmetic according to claim 6, comprising in weight%:

2.0-4.0 parts of retinol, 0.5-2.5 parts of forsythia fruit extract, 0.5-2.5 parts of salvia extract, 2.0-4.0 parts of rhizoma anemarrhenae extract, 4.0-6.0 parts of white willow bark extract, 2.0-4.0 parts of 1,3 propylene glycol, 4.0-6.0 parts of radix sophorae flavescentis extract, 2.0-4.0 parts of soybean lecithin, 2.0-4.0 parts of glucan, 1.0-2.0 parts of nicotinamide and 2.0-4.0 parts of lauroyl arginine ethyl ester.

8. The cosmetic of claim 6, wherein the cosmetic is packaged as a commercially available cosmetic product.

9. The cosmetic of claim 8, wherein said cosmetic further comprises instructions for use of the product.