CN107318839A - A kind of bactericide and its application - Google Patents
A kind of bactericide and its application Download PDFInfo
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- CN107318839A CN107318839A CN201610294258.6A CN201610294258A CN107318839A CN 107318839 A CN107318839 A CN 107318839A CN 201610294258 A CN201610294258 A CN 201610294258A CN 107318839 A CN107318839 A CN 107318839A
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- 239000003899 bactericide agent Substances 0.000 title claims abstract description 20
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- 230000027939 micturition Effects 0.000 description 1
- 150000002780 morpholines Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229960003540 oxyquinoline Drugs 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000009774 resonance method Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 208000005687 scabies Diseases 0.000 description 1
- 239000003620 semiochemical Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- APEJMQOBVMLION-VOTSOKGWSA-N trans-cinnamamide Chemical class NC(=O)\C=C\C1=CC=CC=C1 APEJMQOBVMLION-VOTSOKGWSA-N 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
- 239000012873 virucide Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/16—Coating with a protective layer; Compositions or apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3526—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses the purposes of lansiumamide B or its salt as bactericide.The compound is amide alkaloid isolated in Chinese wampee seed, there is the bactericidal activity of wide spectrum to various plants fungal disease, the preventing and treating of various plants fungal disease can be used for individually or with other composite reagents, especially to rice sheath blight disease, sclerotinia sclerotiorum, grey mould fruit rot of strawberry preventive effect to be more preferable.Meanwhile, it is fresh-keeping that the compound can also be used for seed treatment, medicine fertilizer, the fruits and vegetables flesh of fish.
Description
Technical field
The invention belongs to disinfectant use in agriculture field, and in particular to Lansiumamide B or its salt as bactericide application.
Background technology
Growth of the phytopathogen to plant has very big harm, every to cause huge loss to agricultural every year.For the generation of controlling plant diseases, the loss caused by it is reduced, substantial amounts of antibacterial and Fungicidal active compound is reported and applied.Due to the factor in terms of the resistance to the action of a drug, the research and development of novel germicide are always the focus of each agricultural chemicals enterprise and scientific research personnel's concern.
Calusena lansium (scientific name:Clausena lansium (Lour.) Skeels) Rutaceae, southern china is originated in, is one of fruit, containing abundant vitamin C, sugar, organic acid and pectin.Calusena lansium pericarp and fruit stone can all be used as medicine, eliminate indigestion and phlegm, qi-regulating effect, for accumulation of food in the stomach and intes tine due to indigestion, chest the diaphragm completely disease such as pain, phlegm and retained fluid cough and asthma, and can remove stagnant heat, colic is managed, leaf is pungent in flavor and cool in property, there is expelling wind to resolve the exterior, eliminating phlegm promoting the circulation of qi effect, for preventing and treating the diseases such as influenza, warm disease body heat, cough, asthma, water swelling stomachache, malaria, difficult urination, heat toxin scabies leprosy;Gen Kezhi gas pain and colic.
Wan Shuqing etc. determines Lansiumamide B to Colletotrichum gloeosporioides Penz in Mango and banana blight bacteria bacteriostatic activity using mycelial growth rate method, it was found that under 0.08mg/mL concentration, the compound is respectively 83.33% and 60.78% to the mycelial growth inhibition rate of Colletotrichum gloeosporioides Penz in Mango and banana blight bacteria;Wan Shuqing etc. determines Antifungal Activity in Vitro of the gained compound to Colletotrichum gloeosporioides Penz in Mango using Lansiumamide B as the artificial synthesized 20 kinds of cinnamide compounds of template, it is found that synthesis gained compound activity has no and significantly improves.
Although ten thousand tree green grass or young crops etc. determine Lansiumamide B and its derivative and delivered to Colletotrichum gloeosporioides Penz in Mango and the bacteriostatic activity of banana blight bacteria, and as article, they do not determine Lansiumamide B antimicrobial spectrums or propose any substantive product formula.The present inventor has found that Lansiumamide B have the bactericidal activity of spectrum to a variety of fungies first, especially still has good prevention effect under relatively low metering to rice sheath blight disease, grey mould fruit rot of strawberry and cucumber downy mildew.Also it should be emphasized that the present inventor has found and proposed first applications of the Lansiumamide B in Recompounded pesticide, medicine fertilizer and preservation field.To represent the respect worked ten thousand tree green grass or young crops et al., the present inventor has avoided applications of the Lansiumamide B separately as bactericide in crop subcutaneous ulcer germ and droop in claim 2.
The content of the invention
It is an object of the invention to provide a kind of application of amides compound Lansiumamide B or its salt as disinfectant use in agriculture.
Technical scheme is as follows:
Amides compound of the structural formula as shown in (I) as bactericide application;Structure formula (I) is as follows:
Lansiumamide B of the structural formula of the present invention as shown in (I) is isolated from Chinese wampee seed:
Methanol circumfluence distillation is measured after Chinese wampee seed (10kg) is crushed 3 times for 6 times, 75 degrees Celsius of Extracting temperature, every time 3 hours.Filter and merging filtrate, filtrate decompression is concentrated to give extract medicinal extract 1.8kg.Extract medicinal extract silica gel is with 80-100 mesh silica gel mixed samples, 200-300 mesh silica gel (30 × 250cm) draws section separation, mobile phase selection chloroform/methanol system (chloroform: methanol=100: 0,98: 2,97: 3,95: 5,90: 10,80: 20,70: 30,0: 100) gradient elution, it is divided into 10 fraction sections by thin layer colour developing, is respectively:F1-F10.Wherein F1 sections (0.84kg), with 80-100 mesh silica gel mixed samples, 200-300 mesh silica gel (30 × 250cm) draws section separation, mobile phase selection chloroform/methanol system (petroleum ether: acetone=100: 0,98: 2,97: 3,95: 5,90: 10,80: 20,70: 30,60: 40,40: 60,0: 100) gradient elution, it is divided into 12 fraction sections, respectively F1-1-F1-12 by thin layer colour developing.Wherein F1-3 (0.42kg) recrystallizes to obtain compound L ansiumamide B (23.7g) with petroleum ether/acetone solution
Compound structure identification uses magnetic nuclear resonance method.Each compound physicochemical property and spectral data are as follows:
Lansiumamide B, yellow crystal;1H-NMR(CDCl3, 400MHz) and δ:7.03 (1H, d, J=15.5Hz), 7.63 (1H, d, J=15.5Hz), 6.69 (1H, d, J=8.6Hz), 6.32 (1H, d, J=8.6Hz), 7.28-7.55 (10H, m), 2.97 (3H, s)
Research shows that Lansiumamide B show excellent activity to the various bacteria of agricultural or other field.Therefore, the technical scheme is that Lansiumamide B are used as the purposes for preparing bactericide in agriculture field or other field.
Lansiumamide B or its salt can be used for preventing and treating following disease (including but being not limited to this):Oomycetes diseases, such as downy mildew (cucumber cream enzymophathy, rape frost enzymophathy, soybean frost enzymophathy, beet frost enzymophathy, sugarcane frost enzymophathy, tobacco frost enzymophathy, pea frost enzymophathy, sponge gourd frost enzymophathy, wax gourd frost enzymophathy, muskmelon frost enzymophathy, Chinese cabbage frost enzymophathy, spinach frost enzymophathy, radish frost enzymophathy, oenin enzymophathy, green onion frost enzymophathy), white rust (rape white rust, cabbage white rust), samping off (rape samping off, Tobacco seedling diseases, tomato samping off, capsicum samping off, eggplant samping off, cucumber samping off, cotton seedling samping off), pythium rot (capsicum pythium rot, sponge gourd pythium rot, wax gourd pythium rot), epidemic disease (broad bean epidemic disease, Cucumber Blight, wax gourd epidemic disease, watermelon epidemic disease, muskmelon blight, capsicum epidemic disease, leek epidemic disease, garlic epidemic disease, Cotton blight), late blight (the late blight of potato, tomato late blight) etc.;Fungi Imperfecti disease,Such as droop (sweet potato droop,Cotton wilt,Sesame droop,Castor-oil plant droop,Tomato wilt,Kidney bean droop,Cucumber fusarium axysporum,Sponge gourd droop,Pumpkin droop,Wax gourd droop,Watermelon blight,Muskmelon Fusarium wilt,Capsicum wilt,Faba bean Fusarium wilt,Rape droop,Soybean Fusariuming disease),Root rot (Fusarium solani,Eggplant root rot,Kidney bean root rot,Cucumber root rot,Balsam pear root rot,Cotton root rot,Root rot of Vicia faba),Damping-off (cotton seedling blight,Sesame damping-off,Capsicum damping-off,Cucumber rhizoctonia rot,Chinese cabbage damping-off),Anthracnose,Verticillium wilt (cotton verticillium wilt,Sunflower verticillium wilt,Tomato verticillium wilt,Capsicum verticillium wilt,Eggplant verticillium wilt),Scab (cucurbita pepo scab,Wax gourd scab,Muskmelon scab),Gray mold (cotton boll grey black mildew,Bluish dogbane gray mold,Graw mold of tomato,Botrytis cinerea,Kidney bean gray mold,Celery gray mold,Spinach gray mold,Kiwi berry gray mold,Grey mould fruit rot of strawberry),Brown spot (cotton brown spot,Jute brown spot,Beet cercospora leaf spot,The cercospora brown spot of peanut,Capsicum brown spot,Wax gourd brown spot,Soybean brown spot,Septorial brown spot of sunflower,Pea brown spot,Broad bean brown spot),Black spot (flax vacation black spot,Alternaria stem rot of colza,Sesame black spot,Sunflower black spot,Castor-oil plant black spot,Tomato black spot,Capsicum black spot,Eggplant black spot,Kidney bean black spot,Cucumber black spot,Celery black spot,Black rot of carrot,Melanose or canker of apple,The cercospora black spot of peanut),Spot blight (spotted wilt of tomato,Capsicum spot blight,Celery septoria disease),Early blight (early blight of tomato,Capsicum early blight,Eggplant early blight,Target,Early blight of celery),Ring spot (soybean ring spot,Sesame ring spot,Kidney bean ring spot),Leaf blight (sesame leaf blight,Sunflower leaf rot,Watermelon leaf blight,Muskmelon leaf blight),Base rot disease (tomato base rot disease,Kidney bean base rot disease),And other (Helminthosporium carbonums,Bluish dogbane waist folding disease,Rice blast,The black sheath disease of chestnut,Sugarcane eye spot,Cotton boll aspergillosis,Peanut crown rot,Soybean stem rot,Soybean diplostomiasis,Muskmelon leaf blight,Peanut Web Blotch Disease,The red leaf spot of tea,Pepper white star disease,Wax gourd leaf spot,Celery black rot,Spinach heartrot,Bluish dogbane leaf mold,Bluish dogbane spot disease,Jute stem blight,Purple spot of soybean,Alternaria sesami,Castor-oil plant graywall,Dark brown leaf spot,Cercospora leaf spot of egg plant,Kidney bean red spot disease,Balsam pear Leucoplakia,Watermelon spot disease,Jute withered rotten disease,Sunflower root stem rot,Kidney bean carbon maize ear rot,Soybean target spot disease,Eggplant rod leaf spot,Leaf Spot Caused by Corynespora cassiicola on Cucumber,Leaf muld of tomato,Eggplant leaf mold,Broad bean red spot) etc.;Load fungus diseases, such as rust (stripe rust of wheat, the stem rust of wheat, wheat leaf rust, Peanut Rust, rust of sunflower, sugarcane rust, leek rust, rust of onion, chestnut rust, soybean rust), smut (maize head smut, corn smut, head smut of sorghum, sorghum loose smut, covered kernel smut of kaoliang, sorghum post smut, chestnut kernel smut, smut of sugarcane, Bean rust disease) and other (such as wheat sharp eyespots, rice sheath blight disease) etc.;Sac fungus disease, such as powdery mildew (wheat powdery mildew, rape powdery mildew, sesame powdery mildew, sunflower powdery mildew, beet powdery mildew, eggplant powdery mildew, powdery mildew of pea, sponge gourd powdery mildew, squash marble dust, Pumpkin powdery mildew, wax gourd powdery mildew, melon powdery mildew, uncinula necator, broad bean powdery mildew), sclerotiniose (flax sclerotiniose, sclerotinia sclerotiorum, soybean sclerotinia crown rot, peanut sclerotiniose, tobacco sclerotiniose, capsicum sclerotiniose, eggplant sclerotiniose, bean sclerotinia rot, pea sclerotiniose, cucumber timberrot, balsam pear sclerotiniose, wax gourd sclerotinia, watermelon sclerotiniose, celery sclerotiniose), scab (scab of apple, pear scab) etc..Especially still there is good prevention effect under relatively low metering to rice sheath blight disease, grey mould fruit rot of strawberry and cucumber downy mildew.
Due to its positive characteristic, Lansiumamide B or its salt can utilize the crop important with protecting agriculture and horticulture, domestic animal and breeding stock, and the environment that often goes of the mankind is from the injury of germ.It can utilize fresh-keeping with water fruits and vegetables.
To obtain preferable effect, the consumption of compound changes because of various factors, such as compound used therefor, the crop protected in advance, the type of harmful organism, gradient of infection, weather conditions, application method, the formulation used.
The compound dosage of 10 grams -5 kilograms of per hectare can provide sufficient preventing and treating.
Due to economic and two aspects of synergy, Lansiumamide B or its salt can also be used together with various bactericide.This series bactericidal agent includes but is not limited to following:Amide-type:Flumorph, dimethomorph, tecloftalam, flusulfamide, first flusulfamide, thiophene fluorine bacterium amine, cyflufenamid, Silthiopham, pyrrole metsulfovax, fenhexamid, zoxamide, ring propionyl bacterium amine, tiadinil, zarilamid, double chlorine zarilamid, mefenoxam, benalaxyl-M, carboxin, furan pyrrole acid amides, fenfuram, flutolanil;First propylene and imidazoles:Fluoxastrobin, trifloxystrobin, ZEN 90160, pyraclostrobin, fluoxastrobin, Enestroburin, SSF 126, alkene oxime amine, mepanipyrim, oxime mepanipyrim, probenazole, fluorine bacterium azoles, efficient imazalil, Fenamidone, cyazofamid, imazalil, Prochloraz, evil imidazoles, furidazol, pefurazoate;Triazole type:Oxygen ring azoles, epoxiconazole, Fluquinconazole, Difenoconazole, tetraconazole, Tebuconazole, Flusilazole, simeconazoles, Triadimenol, bitertanol, Cyproconazole, efficient olefin conversion, olefin conversion, Flutriafol, hexaconazole, prothioconazoles, triticonazole, penconazole, nitrile bacterium azoles, RH-7592, glyoxalin, sugared bacterium azoles, kind bacterium azoles, metconazole;Thiazoles:Guardian, benthiozole, octhilinone, Grandox fumigant;Morpholine class:Dodemorph, butadiene morpholine, tridemorph;Pyroles:Fenpiclonil, fludioxonil;Pyridines:Fluazinam, Boscalid, fluopicolide, pyridine bacterium amine, ring pyridine bacterium amine, pyrifenox;Miazines:Pyrimidine cyclammonium, Fenarimol, nuarimol, pyrimethanil, ferimzone, mepanipyrim, fluorine mepanipyrim;Quinoline (azoles) quinoline (ketone) class:Chinomethionat, dithianon, ethoxyquin, 8-hydroxyquinoline, the third oxygen quinoline, quinoxyfen;Carbamates:Iprovalicarb, diethofencarb, benzene metsulfovax, Propamocarb, prothiocarb;Organic phosphates:Edifenphos, different rice blast net, pyrazophos, methyl stand withered spirit;Antibioticses:Tetramycin, blasticidin-S, kasugarnycin, polyoxin, Polyoxin;Other classes:Acibenzolar, K-281, bronopol, benzene flusulfamide, toluene flusulfamide, volution bacterium amine, triforine, fenaminosulf, Pencycuron, Rabcide, tricyclazole, probenazole, fenpropidin, botran, iminoctadine, dodine, cymoxanil, pyroquilon, metrafenone, oxolinic acide, diclomezin;Conventional sterilization agent kind:Streptomysin, metalaxyl, Propineb, M 9834, Yekuzuo, valida, jinggangmeisu, pentachloronitrobenzene, Mancozeb, phosethyl-Al, bupirimate, ethirimol, dimethirimol, triazolone, thiophanate-methyl, carbendazim, benomyl, captan, difoltan, mebenil, folpet, ofurace, fluoromide, vinclozolin, dimethachlon, thiram, Isoprothiolane, kitazine, Bravo, sulphur, Bordeaux mixture, Kocide SD, cuprous oxide, copper oxychloride, copper sulphate;And botanical fungicide:Matrine, Osthole, Gentrin Knotweed P.E, matrine, Osthole, Gentrin Knotweed P.E, camphor, humic acid copper, copper rosinate (sodium), kilnitamin copper, tiger glue adipic acid copper, copper citrate, eugenol, carvacrol, jamaicin, S-Ethyl ethylthio sulfonate, chitosan, oligosaccharide element, Portugal's polyene sugar, allicin etc..
In addition, any combination of above bactericide can be used.
Due to economic and two aspects of synergy, Lansiumamide B or its salt can also be used together with bactericide and herbicide or above two.
Due to economic and two aspects of synergy, Lansiumamide B can also be used together (these types are not mutually exclusive) with acaricide, algicide, anti-feedant, avicide, fungicide, herbicide-safener, herbicide, insect attractant, plant growth regulator, synergist, defoliant, withered agent, disinfectant, semiochemical and virucide.
Due to economic and two aspects of synergy, Lansiumamide B or its salt can prepare patent medicine fertilizer to apply.
The bactericide of respective pure form is less suitable for application.Typically required other materials of addition so that bactericide can needed for concentration and suitable form use, so as to allow the easy and maximum bactericidal activity for applying, handling, transport, storing.
The composition of the present invention can be applied in the form of preparation.Lansiumamide B or its salt are more easy to disperse when being dissolved or dispersed in carrier as active component or be configured to preparation to use as sterilization.For example:These compounds can be made into concentrated emulsion, pulvis, missible oil, aqueous emulsion, paste, disintegrant, fumigant, bait, gel, particle, microcapsules, seed dressing, suspended substance concentrate, suspension emulsion, tablet, water-soluble liquid, water-dispersible grain or dry suspending agent, wettable powder and ultralow bulk solution.In these compositions, a kind of liquid or solid carrier is at least added, and appropriate surfactant can be added when needed.
Further, the bactericidal composition of the present invention is imposed on described germ or its somatomedin.The more suitable effective dose generally selected is 10 grams to 1000 grams of per hectare, and preferred dose is 20 grams to 500 grams of per hectare.
It should be appreciated that, in scope defined by the claims of the present invention, various conversion and change can be carried out.
Embodiment
Embodiment 1:The in vitro biological activity determinations of Lansiumamide B
Compound L ansiumamide B bacteriostatic activities are determined using mycelial growth rate method, 22 pathogen species are chosen altogether, table 4-1 is listed in for each compound inhibiting rate under examination strain and 100ug/ml concentration.With sterilized water configuration testing sample test solution 400ug/ml.Culture medium melts and is cooled to 50 DEG C or so, PDA culture medium by 20ml testing samples test solution respectively with 20ml/ bottles is mixed, the pastille culture medium of corresponding concentration is made, shake up, pour into 3 a diameter of 9cm culture dish rapidly, after after its solidification, it is 0.8cm to take colony diameter, it is central (mycelia is face-down) that the outer layer fungus block of the apple anthracnose opportunistic pathogen of monospore purifying is positioned over culture dish, to add isometric sterilized water as blank control, 50% 500 times of carbendazim liquid compares for agricultural chemicals, each 3 repetitions of processing.After inoculation, after culture 4d in the environment of 30 DEG C, colony diameter is measured using crossing method respectively, using blank control as standard, bacteriostasis rate is calculated as follows.
Colony diameter (mm)=measurement colony diameter average value -4 (bacteria cake diameter)
Lansiumamide B are to 41 kinds of pathogen bacteriostatic activities under table 4-1 200ug/ml concentration
Embodiment 2:The potted plant biological activity determinations of Lansiumamide B
Preventing and treating to wheat powdery mildew, cucumber downy mildew is carried out using potted plant experiment method.Specific method is as follows:By sterile soil be filled to cumulative volume be 220mL dixie cups 3/5ths at, sow wheat.After planting 18 days, when wheat length to two leaf, tested.Test compound Lansiumamide B 500mg/L processing, while setting clear water control and the control of 15% triazolone wp standard agents.Sprayed and pouring root (per basin 10mL) progress dispenser with conventional respectively, method inoculation is shaken off using spore, the wheat after processing is placed in greenhouse and cultivated.Protective effect is tested, and first wheat seedling is sprayed or root irrigation, is inoculated with after 24h.Therapeutic action is tested, and is first inoculated with Powdery Mildew on wheat seedling, is sprayed again or root irrigation after 24h.Each processing sets 6 repetitions, cultivates 7d checkout facility results, and prevention effect, measurement result such as table 4-2 is calculated as follows.
Potted plant test of pesticide effectiveness results (preventive effect) of the table 4-2 Lansiumamide B to wheat powdery mildew, cucumber downy mildew
Embodiment 3:Lansiumamide B and other bactericide compounded in vitro biological activity determinations
The embodiment is determined using mycelial growth rate method, described in method be the same as Example 1.1 is Lansiumamide B in sample, and concentration is classified as Lansiumamide B concentration+compound drug concentration.Bacteriostasis rate measurement result such as table 4-3.
Table 4-3 Lansiumamide B are with representative bactericide to 7 kinds of pathogen bacteriostasis rates
Embodiment 4:Lansiumamide B medicine fertilizer is added to rice application effect
Test and carried out in 2001 in Hanzhong, for trying soil for river wattenshlick, the content of organic matter 1620g/kg, quick-acting nitrogen content 13788mg/kg, available phosphorus contents 5872mg/kg, quick-acting potassium content 5247mg/kg, pH value is 6.24.Be gold excellent 207 for examination rice varieties, test in sowing on May 10, June 18 field-transplanting, seeding row spacing is 13cm*20cm, and September is gathered on the 8th.
The experiment of each compound sets 4 processing:(1) medicine (Lansiumamide B);(2) it is fertile;(3) medicine fertilizer [fertilizer of the autogamy with medicine+(2) equivalent of (1) equivalent];(4) CK (clear water control).By seed rice by processing difference presoaking and germinating, in rice seedling bed nursery, crop field is transplanted respectively by processing, and the interval sprayed in tillering stage and heading stage office reason per cell between 2 times, 2 times is 5d.It is repeated 4 times, 16 cells is set altogether, crop field plot area is 20m2, random district's groups arrangement.Field management:Each processing base manure topdresses unanimously, using conventional field management method.The seed soaking concentration of compound 1 is 300ug/ml in (I) in this experiment, and spraying concentration is 300ug/ml;Fertilizer is by (NH4)2PO4、KNO3、ZnSO4Mixture is formed, and it is soaked seed and the spraying concentration in two periods is respectively 1%, 1.2% and 0.8%.
Test result indicates that:Rice paddy seed average germination percentage processing (3) improves 2.36% than control, illustrates that germination of the medicine fertilizer processing to seed has facilitation.The root activity for handling (1) is minimum, handles the root activity highest of (2), the difference of root activity is not obvious between processing (3) and processing (4).Rice yield is respectively handled all apparently higher than control, wherein to handle (3) for highest, processing (2) is taken second place, both are respectively than control volume increase 6.46% and 3.75%.It is preferably, secondly processing (1) to the prevention effect of rice sheath blight disease to handle (3).
Embodiment 5:Lansiumamide B are to matrimony vine fresh-keeping effect
10 fruits are randomly selected from each processing, through smashing crusher machine to pieces, low-temperature centrifugation takes supernatant to carry out fruit quality analysis.Soluble solid content (soluble solids content, SSC) is measured with digital display refractometer, each parallel determination 10 times, is averaged.Titrable acidity (titratable acidity, TA) uses determination of acid-basetitration;Anthrone colorimetry determines total sugar content (total sugar content, TSC);The content of 2,6- dichloroindophenol titration measuring ascorbic acid (ascorbic acid, AsA);Above Indexs measure result is the average value of 3 parallel determinations.
Experimental result is shown, compared with the control, after matrimony vine is handled 6 days using Lansiumamide B, show certain fresh-keeping effect, it significantly suppress postharvest decay and the moisture loss of fruit, the decline of soluble solid, titratable acid and ascorbic acid content is delayed, little is influenceed on the content of soluble sugar;Compelx coating processing can also keep the activity of higher superoxide dismutase, peroxidase, chitinase, β -1,3- dextranases and phenylalanine lyase, influence little to polyphenol oxidase activity.
Embodiment 6:Lansiumamide B are evaluated pork fresh-keeping effect
Trunk after live hog is slaughtered suddenly freezes 1.5~2h of cooling in less than -23 DEG C immediately, the chilling room for being transferred to 0~4 DEG C again keeps 6~8h, when the central temperature of meat is dropped to below 10 DEG C, it is transferred between 10~15 DEG C of segmentations and pick a bone segmentation, hind leg muscle (biceps muscle of thigh) is taken to cut into 150g or so fritter, every group 30 pieces, every group of cube meat in (I) of its corresponding various concentrations compound 1-11 (500, 250, 125ug/ml) impregnated in aseptic aqueous solution and sterilized water after 3~5s, taking-up is put in clean riddle, 3~5min of draining, it is then charged into polythene film bag, vacuum packaging, stick the label of packet, it is stored refrigerated in 0~4 DEG C of precooling room.
Result of study is shown:After Chilled Meats are handled 6 days using Lansiumamide B, certain fresh-keeping effect is shown, meat has faint peculiar smell, and color and luster, elasticity, viscosity are without significant change;PH value becomes big to 4.7;The changing rule for being waterpower is first slightly decreased it is obvious that substantially having, then slight rise downward trend again again;The Chilled Meats fresh-keeping effect of VBN (TVB values) treatment group is substantially better than control group;Total number of bacteria treatment group is substantially better than control group.Compound 1-11 described above can apply to meat preservation.
Claims (10)
1. amides compound Lansiumamide B or its salt are used as the application of bactericide.
2. the application described in right 1, it is characterised in that Lansiumamide B or its salt are used as the following disease of bactericidal agent for preventing and treating:Downy mildew (cucumber
The white enzymophathy of white enzymophathy, rape, the white enzymophathy of soybean, the white enzymophathy of beet, the white enzymophathy of sugarcane, the white enzymophathy of tobacco, the white enzymophathy of pea, the white enzyme of sponge gourd
The white enzymophathy of disease, wax gourd, the white enzymophathy of muskmelon, the white enzymophathy of Chinese cabbage, the white enzymophathy of spinach, the white enzymophathy of radish, oenin enzymophathy, the white enzymophathy of green onion), in vain
Rest fungus (rape white rust, cabbage white rust), samping off (rape samping off, Tobacco seedling diseases, tomato samping off, capsicum samping off,
Eggplant samping off, cucumber samping off, cotton seedling samping off), pythium rot (capsicum pythium rot, sponge gourd pythium rot, wax gourd pythium rot), epidemic disease (silkworm
Beans epidemic disease, Cucumber Blight, wax gourd epidemic disease, watermelon epidemic disease, muskmelon blight, capsicum epidemic disease, leek epidemic disease, garlic epidemic disease, Cotton blight),
Late blight (late blight of potato, tomato late blight) etc.;Root rot (Fusarium solani, eggplant root rot, Kidney bean root rot, cucumber root
Maize ear rot, balsam pear root rot, cotton root rot, root rot of Vicia faba), damping-off (cotton seedling blight, sesame damping-off, capsicum damping-off, Huang
Melon damping-off, Chinese cabbage damping-off), (cotton verticillium wilt, sunflower verticillium wilt, tomato verticillium wilt, capsicum verticillium wilt, eggplant Huang wither verticillium wilt
Disease), scab (cucurbita pepo scab, wax gourd scab, muskmelon scab), gray mold (cotton boll grey black mildew, bluish dogbane gray mold, kind
Solanum cinerea, Botrytis cinerea, Kidney bean gray mold, celery gray mold, spinach gray mold, Kiwi berry gray mold, grey mould fruit rot of strawberry), foxiness
Disease (cotton brown spot, jute brown spot, beet cercospora leaf spot, the cercospora brown spot of peanut, capsicum brown spot, wax gourd brown spot, soybean brown spot,
Septorial brown spot of sunflower, pea brown spot, broad bean brown spot), black spot (flax vacation black spot, alternaria stem rot of colza, sesame black spot, Xiang
Certain herbaceous plants with big flowers black spot, castor-oil plant black spot, tomato black spot, capsicum black spot, eggplant black spot, Kidney bean black spot, cucumber black spot, celery are black
Pinta, black rot of carrot, melanose or canker of apple, the cercospora black spot of peanut), spot blight (spotted wilt of tomato, capsicum spot blight, celery septoria disease),
Early blight (early blight of tomato, capsicum early blight, eggplant early blight, target, early blight of celery), ring spot (soybean ring spot,
Sesame ring spot, Kidney bean ring spot), leaf blight (sesame leaf blight, sunflower leaf rot, watermelon leaf blight, muskmelon leaf blight), stem foot
Maize ear rot (tomato base rot disease, Kidney bean base rot disease), and other (Helminthosporium carbonum, bluish dogbane waist folding disease, rice blast, the black sheath disease of chestnut, sugarcanes
Eye spot, cotton boll aspergillosis, peanut crown rot, soybean stem rot, soybean diplostomiasis, muskmelon leaf blight, Peanut Web Blotch Disease, the red tikka of tea
Disease, pepper white star disease, wax gourd leaf spot, celery black rot, spinach heartrot, bluish dogbane leaf mold, bluish dogbane spot disease, jute stem blight,
Purple spot of soybean, Alternaria sesami, castor-oil plant graywall, dark brown leaf spot, cercospora leaf spot of egg plant, Kidney bean red spot disease, balsam pear Leucoplakia,
Watermelon spot disease, jute withered rotten disease, sunflower root stem rot, Kidney bean carbon maize ear rot, soybean target spot disease, eggplant rod leaf spot, Leaf Spot Caused by Corynespora cassiicola on Cucumber,
Leaf muld of tomato, eggplant leaf mold, broad bean red spot) etc.;Load fungus diseases, such as rust (stripe rust of wheat, the stem rust of wheat, wheat
Leaf rust, Peanut Rust, rust of sunflower, sugarcane rust, leek rust, rust of onion, chestnut rust, soybean rust), smut (corn
Head smut, corn smut, head smut of sorghum, sorghum loose smut, covered kernel smut of kaoliang, sorghum post smut, chestnut kernel smut,
Smut of sugarcane, Bean rust disease) and other (such as wheat sharp eyespots, rice sheath blight disease) etc.;Sac fungus disease, such as powdery mildew (wheat
Powdery mildew, rape powdery mildew, sesame powdery mildew, sunflower powdery mildew, beet powdery mildew, eggplant powdery mildew, powdery mildew of pea, sponge gourd are white
Powder disease, squash marble dust, Pumpkin powdery mildew, wax gourd powdery mildew, melon powdery mildew, uncinula necator, broad bean powdery mildew), sclerotiniose is (sub-
Numb sclerotiniose, sclerotinia sclerotiorum, soybean sclerotinia crown rot, peanut sclerotiniose, tobacco sclerotiniose, capsicum sclerotiniose, eggplant sclerotiniose, Kidney bean bacterium
Core disease, pea sclerotiniose, cucumber timberrot, balsam pear sclerotiniose, wax gourd sclerotinia, watermelon sclerotiniose, celery sclerotiniose), scab (apple
Fruit scab, pear scab), clubroot (club-root, cabbage clubroot, cauliflower clubroot, skim blue clubroot, leaf mustard clubroot,
Radish root tumefacting disease, turnip clubroot, rapeseed clubroot).
3. a kind of utilize Lansiumamide B or its salt to be used for the application for preparing microbicide compositions.
4. a kind of composition and this composition are used as the application of the application of bactericide.It is characterized in that:Composition is included in claim 1
Lansiumamide B or its salt and other at least one bactericide composition.
5. a kind of application of composition and this composition in agricultural production.It is characterized in that:Composition includes Lansiumamide in claim 1
B or its salt and at least one insecticide, a kind of herbicide or at least one insecticide and herbicide.
6. a kind of composition and this composition are used as the application of bactericide.It is characterized in that:Lansiumamide in claim 1 is included in composition
B or its salt are selected from following member with least one:Flumorph, dimethomorph, tecloftalam, flusulfamide, first flusulfamide, thiophene fluorine bacterium amine,
Cyflufenamid, Silthiopham, pyrrole metsulfovax, fenhexamid, zoxamide, ring propionyl bacterium amine, tiadinil, zarilamid, double chlorine cyanogen bacterium
Amine, mefenoxam, benalaxyl-M, carboxin, furan pyrrole acid amides, fenfuram, flutolanil, Fluoxastrobin, trifloxystrobin, ZEN 90160,
Pyraclostrobin, fluoxastrobin, Enestroburin, SSF 126, alkene oxime amine, mepanipyrim, oxime mepanipyrim, probenazole, fluorine bacterium azoles, efficiently
Imazalil, Fenamidone, cyazofamid, imazalil, Prochloraz, dislike imidazoles, furidazol, pefurazoate, oxygen ring azoles, epoxiconazole, Fluquinconazole,
Difenoconazole, tetraconazole, Tebuconazole, Flusilazole, simeconazoles, Triadimenol, bitertanol, Cyproconazole, efficient olefin conversion,
Olefin conversion, Flutriafol, hexaconazole, prothioconazoles, triticonazole, penconazole, nitrile bacterium azoles, RH-7592, glyoxalin, sugared bacterium azoles, kind bacterium azoles,
Metconazole;Thiazoles:Guardian, benthiozole, octhilinone, Grandox fumigant, dodemorph, butadiene morpholine, tridemorph;Pyrroles
Class:Fenpiclonil, fludioxonil, fluazinam, Boscalid, fluopicolide, pyridine bacterium amine, ring pyridine bacterium amine, pyrifenox, pyrimidine cyclammonium, chlorine
Benzene ancymidol, nuarimol, pyrimethanil, ferimzone, mepanipyrim, fluorine mepanipyrim, chinomethionat, dithianon, ethoxyquin, 8- hydroxyls
Base quinoline, the third oxygen quinoline, quinoxyfen, iprovalicarb, diethofencarb, benzene metsulfovax, Propamocarb, prothiocarb, edifenphos, different rice blast net,
Pyrazophos, methyl found withered spirit, tetramycin, blasticidin-S, kasugarnycin, polyoxin, Polyoxin, Acibenzolar, K-281, bromine nitre
Alcohol, benzene flusulfamide, toluene flusulfamide, volution bacterium amine, triforine, fenaminosulf, Pencycuron, Rabcide, tricyclazole, probenazole,
Fenpropidin, botran, iminoctadine, dodine, cymoxanil, pyroquilon, metrafenone, oxolinic acide, diclomezin, streptomysin, metalaxyl,
Propineb, M 9834, Yekuzuo, valida, jinggangmeisu, pentachloronitrobenzene, Mancozeb, phosethyl-Al, ethirimol sulfonic acid
Ester, ethirimol, dimethirimol, triazolone, thiophanate-methyl, carbendazim, benomyl, captan, difoltan, mebenil, folpet,
Ofurace, fluoromide, vinclozolin, dimethachlon, thiram, Isoprothiolane, kitazine, Bravo, sulphur, Bordeaux mixture,
Kocide SD, cuprous oxide, copper oxychloride, copper sulphate, matrine, Osthole, Gentrin Knotweed P.E, camphor, humic acid copper, rosin
Sour copper (sodium), kilnitamin copper, tiger glue adipic acid copper, copper citrate, eugenol, carvacrol, jamaicin, S-Ethyl ethylthio sulfonate, chitosan,
Oligosaccharide element, Portugal's polyene sugar, allicin.
7. a kind of application including applying Lansiumamide B or its salt in claim 1 to seed.
8. the application of the medicine fertilizer of Lansiumamide B or its salt in a kind of claim 1 including addition.
9. it is a kind of including applying in claim 1 Lansiumamide B or its salt to fruit and vegetable, fish, meat with fresh-keeping application.
It is committed to government organs to obtain comprising related described in claim any one of 1-9 10. one kind includes being related to applying described in claim 1-9
Equipment registration license method.
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