A kind of control method of Carrot Roots maize ear rot caused by alternaria solani sorauer or leaf spot
Technical field
The present invention relates to plant disease technical field, Hu trailing plants caused by more particularly to a kind of alternaria solani sorauer
Foretell the control method of root rot or leaf spot.
Background technique
The generation of carrot leaf pinta aggravates year by year with harm, has become and restricts one of carrot yield and quality most
Important factor.It, usually only should in early stage control of medication by chemical prevention approach due to lacking basic research in production
Disease;But the Species of Pathogens of domestic report is more at present, and mostly without passing through stringent identification, people are often in uncertain cause of disease
In the case where, blindness medication causes the disease that cannot effectively control, and becomes the problem in carrot production.It has been reported recklessly
Radish leaf spot is that bacillary, carrot tail spore bacterium causes, and uses Bravo, carbendazim, pesticide agricultural streptomycin and some
Foliar fertilizer carries out prevention and control, but does not all control the disease over 10 years effectively.
The present invention passes through pathogen identification early period and analysis, it is believed that in the Northeast, carrot leaf pinta is by alternaria solani sorauer
Caused by (Alternaria solani), start to fall ill at the beginning of general 7 the end of month August in northeast, such as prevention and treatment is easily expanded rapidly not in time
Exhibition sprawling, serious Shi Quantian blade of falling ill is withered and yellow, in shape is burnt, can cause the carrot significantly underproduction.Alternaria solani sorauer can seed belt
Bacterium, the seed for influencing carrot is sprouted, and endangers root, blade and the fruit of carrot, causes root rot, causes blade withered
Death, fruit is less than normal and necrotic plaque occurs.
Therefore, how for specific pathogen bacterium alternaria solani sorauer effectively preventing for a kind of Carrot Roots maize ear rot or leaf spot is provided
Method becomes this field technical problem urgently to be resolved.
Summary of the invention
In view of this, the present invention starts with from seed-borne fungi, pass through seed soaking, pelletized processing and field spray processing
Diversification chemicals treatment mode, can effectively Carrot Roots maize ear rot or leaf spot caused by prevention and control alternaria solani sorauers generation.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of control method of Carrot Roots maize ear rot caused by alternaria solani sorauer or leaf spot, including handle as follows:
(1) seed soaking is carried out to Carrot Seed using benzene first Fluoxastrobin;
(2) using benzene first Fluoxastrobin mix pelletized pulvis to after seed soaking seed carry out it is pelletized, it is pelletized
After planted;
(3) spraying treatment is carried out to the Carrot middle and later periods using the medicament containing benzene first Fluoxastrobin.
325 grams per liter of active constituent content of benzene first Fluoxastrobin, wherein 125 grams per liter of difenoconazole, Fluoxastrobin 200
Grams per liter.
Preferably, the specific method is as follows for seed soaking in processing (1): using the 500 of 325 grams per liter benzene first Fluoxastrobins
Times liquid handles 5.5-6.5h to seed-soaking, dries in the shade.
Preferably, it is as follows to handle pelletized pulvis number scale component by weight in (2): 25-35 parts of talcum powder, kaolin
14-18 parts, 25-35 parts of silica, 14-18 parts of bentonite, 6.0-6.5 parts of polyvinyl alcohol, wooden calcium sulfonate 0.25-0.35 parts, carboxylic
1.2-1.8 parts of sodium carboxymethylcellulose pyce.
Preferably, it is as follows to handle pelletized pulvis number scale component by weight in (2): 30 parts of talcum powder, 16 parts of kaolin,
30 parts of silica, 16 parts of bentonite, 6.2 parts of polyvinyl alcohol, 0.3 part of wooden calcium sulfonate, 1.5 parts of sodium carboxymethylcellulose.
Preferably, pelletized in processing (2) the specific method is as follows: taking 3 kilograms of pelletized pulvis, Carrot Seed 1,000
Gram, 12 milliliters of Fluoxastrobin of 325 grams per liter benzene first be dissolved in 2000 milliliters of water, be put into pellet machine and pill pelleting seed be made.
Preferably, handle (3) in after emergence the 42-48 days progress first time spraying treatments;The 77-83 days after emergence
Carry out second of spraying treatment.The carrot leaf pinta later period is mostly to fall ill for 85 days or so after emerging, and therefore, is not carrying out period of disease
Between sensitive stage carry out twice spraying treatment can effectively prevent the generation of leaf spot.
Preferably, benzene first Fluoxastrobin is mixed with organosilicon auxiliary agent when handling spraying treatment in (3) and is sprayed;Benzene
First Fluoxastrobin dosage 850-950ml/ha, organosilicon auxiliary agent dosage 400-500ml/ha.By benzene first Fluoxastrobin and organosilicon
Auxiliary agent is mixed can to enhance drug effect.
As shown from the above technical solution, the present invention is to the pathogen alternaria solani sorauer for causing Carrot Roots maize ear rot or leaf spot
On the basis of carrying out separation identification, it is found that seed-borne fungi and field invalid body are main primary source of infection, therefore, the method for the present invention from
Pathogen is started with, by seed treatment (kill Interior Seed pathogen, reduce root rot generation and later period leaf spot just
Source of infection), it is pelletized processing (dispose most of pathogen in the seed surrounding soil of field on invalid body, and reduce root-rot
Disease generation) and emergence after spraying treatment, effectively control the generation of the disease.
Detailed description of the invention
Fig. 1 show carrot leaf pinta symptom caused by alternaria solani sorauer;
Fig. 2 show Carrot Roots maize ear rot symptom caused by alternaria solani sorauer;
Fig. 3 show alternaria solani sorauer conidium form;
Fig. 4 show alternaria solani sorauer hypha form;
Fig. 5 show alternaria solani sorauer Molecular Identification electrophoretogram;
Wherein, left side swimming lane is marker, and right lanes are alternaria solani sorauer amplified sample;
Fig. 6 show complete Carrot Seed seed borne fungi;
Fig. 7 show chopping Carrot Seed seed borne fungi;
Fig. 8 show field trial plant picture;
Wherein, the picture left above is control group, and top right plot is Fluoxastrobin group, and lower-left figure is the third ring Fluoxastrobin group;Bottom-right graph is
Benzene first Fluoxastrobin group.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The separation and identification of 1 pathogen of embodiment
1. pathogenicbacteria separation
Fig. 1,2 show carrot leaf pinta and root rot symptom, take its carrot disease leaf disease be good for intersection diseased tissues and
Carrot Seed is put into 0.1% mercuric chloride after processing 3min, and sterile water is changed clothes 3 times;It will treated diseased tissues and complete kind
Son is placed on PDA plate, 25 DEG C of culture 5d, the single bacterium colony of tissue surrounding on culture medium is selected, in a little bacterium of colony edge picking
Silk is stored in test tube after purification for morphology and molecular biology identification.
2. Morphological Identification
The pathogen that the pathogen separated from sick leaf texture and Interior Seed separate is completely the same from form (Fig. 3,4).
Mycelium all buries the life of raw or part table body, hyphae colorless to brown, seldom formation stroma, no bristle and attached branch.Mitogenetic spore
Son stalk is filbert to brown, and single or irregular branch are short or long, are bent or in bending knee shape.Conidiogenous cell hole goes out formula and produces spore, closes
Shaft type extends or does not re-extend, and spore trace is obvious.Conidium is in chain or Dan Sheng, and filbert to dark brown, shape is different, surface
Smooth or tool aculea, has diaphragm in length and breadth, top is often with beak.Mycelia has diaphragm and branch, and older color is deeper.Conidiophore
It is upright or slightly bent, color depth and it is short, Dan Sheng or grow thickly, there is 1-7 diaphragm, size is 6-9 μm of 50-90 μ m.Conidium is certainly
Conidiophore top generates, and usual Dan Sheng, shape difference is very big, the shape of falling club to oblong, and color is light golden yellow
To olive brown, has long howl, surface is smooth, 9-11 diaphragm, and 0 arrives several mediastinum films, and size is 117-154 μ m 9.8-15.7
μm, howl is long to be equal or longer than spore body, there is branch sometimes, and howl is 2.5-5 μm wide.
3. Molecular Identification
In order to guarantee the accuracy of strain idenfication, one plant of representativeness will be selected in the bacterial strain that morphology Preliminary Identification is completed
Strain culturing 5-6d scrapes the mycelia on bacterium colony upper layer, and health is used to carry out DNA extraction for century DNA kit.By the DNA of extraction
It is expanded with fungi universal primer (ITS1/ITS4), primer are as follows:
ITS1:5'-TCCGTAGGTGAACCTGCGG-3'
ITS4:5'-TCCTCCGCTTATTGATATGC-3'.
Amplification system (50 μ l) such as table 1:
Table 1
PCR response procedures such as table 2:
Table 2
As a result it detects: 5 μ l amplified productions being taken to be detected through 1% agarose gel electrophoresis after the reaction was completed.Electrophoresis detection
Band meets the requirements, as a result clear (Fig. 5), serves Hai Shenggong and amplified production is sequenced, gene order is in ncbi database
It is online to compare.
It is compared on Blast after amplification sequencing and thinks the bacterial strain and Alternaria solani strain IA300 (sequence
Row number AY154716.1) for consistency up to 98%, Molecular Identification is alternaria solani sorauer (Alternaria solani).
By morphology and molecular biology identification, determines and the pathogen of carrot leaf pinta and root rot is caused to be eggplant chain
Lattice spore (Alternaria solani).
The different carrot variety seed transmission ratio detections of embodiment 2
10 kinds progress bacterial bearing rate detection to the main cultivation in Shuangyashan area, 30 seeds of each variety experiment, every 10 1
A repetition, specific separation method the results are shown in Table 3 with embodiment 1.
Seed/measurement seed sum × 100% for bacterial bearing rate (%)=carry disease germs.
Table 3
As shown in Table 3, six cun of ginseng bacterial bearing rates of Han Hong are up to 60.0%, are secondly happy name way, bacterial bearing rate 23.3%,
Its 8 breed difference is not significant.The fungal bacterial strain being separated to is identified, is same class fungi with the bacterial strain separated before,
It is all alternaria solani sorauer (Alternaria solani).
3 seed transmission ratio of embodiment and location detection of carrying disease germs
Experimental cultivar: Shuangyashan area main breed " six cun of Han Hong ginsengs ".
For trying culture medium: potato dextrose medium (PDA).
By the surface of the seed after 70% ethanol postincubation 2s, it is put into after handling 3min in 0.1% mercuric chloride, sterile water is changed clothes 3 times,
30 (10/ware) of every processing are placed on PDA plate, 30 complete seeds are put after 30 are cut off with sterilizing scissors from centre
On PDA plate;25 DEG C of culture 5d are observed whether there is or not fungus colony appearance, and test is repeated once.
Table 4
As shown in Table 4, it will be cultivated after whole seed and the processing of the seed asepsis of incision, bacterial bearing rate is 53.3%, no
There are significant difference, illustrates that seed-borne fungi position is the shallow-layer of Ying's shell, also indicate that seed transmission ratio is higher (Fig. 6,7).
The seed soaking of embodiment 4 and pelletized processing
Experimental cultivar: Shuangyashan area main breed " six cun of Han Hong ginsengs ".
Reagent agent:
Fluoxastrobin: 250 grams per liter Fluoxastrobin suspending agents, Shanghai Shanghai join biological medicine company (Xiayi) limited liability company;
Boscalid: 50% Boscalid water dispersible granules, BASF European Co., Ltd;
Difenoconazole: 10% difenoconazole water dispersible granule, Syngenta Nantong crop protection Co., Ltd;
Carbendazim: 80% carbendazol wettable powder, Shandong sea and three sharp biochemical industry Co., Ltds;
Third ring Fluoxastrobin: the 18.7% the third ring Fluoxastrobin suspending agents, Swiss Syngenta Crop Protection Co., Ltd.;
Benzene first flutolanil: 12% benzene first flutolanil suspending agent, BASF European Co., Ltd;
Captan: 80% captan water dispersible granule, An Daomai Maxime Co., Ltd;
Fludioxonil: 25 grams per liter fludioxonil suspension seed-coating agents, Syngenta Nantong crop protection Co., Ltd;
Benzene first Fluoxastrobin: 325 grams per liter benzene first Fluoxastrobin suspending agents, Syngenta Nantong crop protection Co., Ltd.
Pelletized pulvis: every 100g 30g containing talcum powder, kaolin 16g, silica 30g, bentonite 16g, polyvinyl alcohol
6.2g, wooden calcium sulfonate 0.3g, sodium carboxymethylcellulose 1.5g are 2000 mesh superfine powders.
For test-run a machine tool:
The pelletized machine of Carrot Seed: moisten rich r-325 type pellet machine, the production of Qingdao Run Defeng agricultural machinery Co., Ltd.
Different reagent agents are diluted to 500 times of liquid for seed soaking respectively, and using sterile water as control;By carrot
Seed is immersed, and is kept 6h, is dried in the air out after taking-up;It 1 kilogram of Carrot Seed after taking 3 kilograms of pelletized pulvis, seed soaking, takes
325 12 milliliters of Fluoxastrobin of grams per liter benzene first are dissolved in 2000 milliliters of water, are put into pelletized machine and pill pelleting seed is made.
Every processing takes 60 pill pelleting seeds, wherein 30 are used to measure influence of the reagent agent to seed transmission ratio, band
The detection of bacterium rate uses cultivation, after the surface of the seed is sterilized, is placed in PDA culture medium and cultivates, 10 tablets each time, is repeated 3 times, and calculates
Bacterial bearing rate.In the culturing pot with sterilized soil, 10, every alms bowl, 3 alms bowls of every processing above cover 1cm soil, are put in temperature other 30 kinds
After cultivating 15d in room, investigation plant height, germination percentage and fresh weight.
Seed/measurement seed sum × 100% for bacterial bearing rate (%)=carry disease germs
Preventive effect (%)=(control group bacterial bearing rate-reagent agent processing group bacterial bearing rate)/control group bacterial bearing rate × 100%
500 times of liquid processing experimental results of different reagent agents are shown in Table 5.
Table 5
As can be seen from Table 5, difference is influenced on seed-borne fungi after 9 processing group seed soaking, wherein benzene first Fluoxastrobin preventive effect
Preferably up to 100%, secondly, Fluoxastrobin and the third ring Fluoxastrobin preventive effect are 40% or more, other medicament fungistatic effects with compare it is poor
It is different not significant.If can in view of improving Drug level to the drug resistance of field conditions, carrot safety and pathogen
It has an impact, therefore benzene first Fluoxastrobin, Fluoxastrobin and the third ring Fluoxastrobin is selected to carry out subsequent field efficacy measurement.
Influence of the different seed soaking times of embodiment 5 to seed transmission ratio and growth
When Carrot Seed is impregnated different in 500 times of liquid of benzene first Fluoxastrobin (325 grams per liter of active constituent content)
Between after (2,4,6,8h), (investigation method is with embodiment 4) is investigated in the influence for carrying out bacterial bearing rate and growth, sterile water seed soaking as pair
According to the results are shown in Table 6.
Table 6
As can be seen from Table 6, processing 6h and processing 8h is respectively 90.3% and 100.0% to the preventive effect of seed-borne fungi, and
Difference is not significant, and not significant with other processing differences on germination percentage and fresh weight influence, and on plant height influence, there is some difference, from
For point of view of practicability, 6h operability is more preferable, and handles 6h compared with the control, and significant difference is not present, and 8h is to plant height
Generate certain influence.Comprehensive analysis, Medicament soaked seed handle 6h, and effect is best.
The seed soaking of embodiment 6 and pelletized processing influence seed and growth of seedling
Experimental cultivar: Shuangyashan area main breed " six cun of Han Hong ginsengs ".
Reagent agent:
Benzene first Fluoxastrobin: 325 grams per liter benzene first Fluoxastrobin suspending agents, Syngenta Nantong crop protection Co., Ltd.
Processing 1 is 500 times of liquid seed soaking of benzene first Fluoxastrobin, pelletized seed;Processing 2 is benzene first Fluoxastrobin
500 times of liquid seed soaking, non-pelletized seeds, processing 3 are sterile water seed soaking, pelletized seed, compare and soak seed for sterile water, is non-
Pill pelleting seed.Seed soaking, pelletized step are the same as embodiment 4.
300 kinds of every processing are in the culturing pot with sterilized soil, 100, every alms bowl (20cm culturing pot), 3 alms bowls of every processing,
On cover 1cm soil, be put in after cultivating 15d in greenhouse, investigation plant height, germination percentage, fresh weight and root rot disease incidence, investigation result are shown in
Table 7.
Preventive effect (%)=(control group root rot disease incidence-processing group root rot disease incidence)/control group root rot disease incidence
× 100%
Table 7
As can be seen from Table 7, each processing effectively increases the germination rate of seed compared with the control, at the same effectively control because
The generation for the root rot that seed-borne fungi generates;From apparently and from the point of view of measurement, there is no phytotoxicity production to the normal growth of seed
It is raw, comprehensively consider each processing, 500 times of liquid seed soaking of benzene first Fluoxastrobin, pelletized seed are generally optimal processing,
It is worth being applied in production.
The field control of 7 carrot leaf pinta of embodiment
Experimental cultivar: Shuangyashan area main breed " six cun of Han Hong ginsengs ".
Reagent agent:
Fluoxastrobin: 250 grams per liter Fluoxastrobin suspending agents, Shanghai Shanghai join biological medicine company (Xiayi) limited liability company;
Third ring Fluoxastrobin: the 18.7% the third ring Fluoxastrobin suspending agents, Swiss Syngenta Crop Protection Co., Ltd.;
Benzene first Fluoxastrobin: 325 grams per liter benzene first Fluoxastrobin suspending agents, Syngenta Nantong crop protection Co., Ltd;
Organosilicon auxiliary agent: 99.8% pure organosilicon synergist, Hebei Nong Xun Biotechnology Co., Ltd.
For test-run a machine tool:
The pelletized machine of Carrot Seed: moisten rich r-325 type pellet machine, the production of Qingdao Run Defeng agricultural machinery Co., Ltd;
Digital control seed braider: RC-5D type, the production of Qingdao Run Defeng agricultural machinery Co., Ltd.
Seed soaking 6h is carried out to Carrot Seed using 500 times of liquid of three kinds of reagent agents, takes pellet after seed soaking
1 kilogram of Carrot Seed after changing 3 kilograms of pulvis, seed soaking, 12 milliliters of Fluoxastrobin of 325 grams per liter benzene first is taken to be dissolved in 2000 milliliters
In water, it is put into pelletized machine and pill pelleting seed is made.Then seed braider is utilized, seed is incorporated into rope, plants field,
Seed spacing 5.6cm, row spacing 65cm, duplicate rows plantation, line-spacing 15cm, depth 1.5cm.
The seed in rope will be incorporated into plant using professional planting machinery to field, the 45d and 80d2 after emergence
Secondary progress field spray processing.Each processing group is sprayed using reagent agent addition organosilicon auxiliary agent used of soaking seed, and uses agent
Amount is shown in Table 8;Every 0.5 mu of 8 ridge 60m of processing, with water 30L, 3 repetitions, 85d investigates field incidence after emergence, after emergence
100d harvesting, which is surveyed, to be produced, as a result as shown in table 8, Fig. 8.Control group is without seed soaking, pelletized and spraying treatment.
Carrot leaf pinta severity Scaling standard is 0 grade: disease-free spot;1 grade: lesion area is below 5%;2 grades: scab face
Product is in 5%-25%;3 grades: lesion area is in 25%-50%;4 grades: lesion area is at 50%~75%:5 grades: lesion area
75% or more.
Disease index=(the ∑ numbers of blade at different levels × state of an illness rank)/(5 × investigation total leaf number) × 100
Disease incidence=morbidity strain number/processing strain number × 100%
Preventive effect (%)=(control disease index-processing disease index)/control disease index × 100%
As shown in Table 8, Fluoxastrobin, the third ring Fluoxastrobin and benzene first Fluoxastrobin processing group are substantially better than control, can be effective
Control the generation of carrot leaf pinta, field does not have phytotoxicity generation, and the plant above ground portion cauline leaf of reagent agent processing (see Fig. 8)
Clear superiority adjoining tree, yield promoted it is obvious, can replace the effective agent as the prevention and treatment of carrot leaf pinta come using, with
The generation of anti-drug resistance.
As shown in figure 8, benzene first Fluoxastrobin processing group plant color is better than remaining two groups.The plant color of three processing groups
It is significantly better than control group.
Table 8
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.