CN107314960A - A kind of hematocrite concentration sensor and preparation method thereof, test device - Google Patents
A kind of hematocrite concentration sensor and preparation method thereof, test device Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
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- 230000007797 corrosion Effects 0.000 claims abstract description 31
- 238000005530 etching Methods 0.000 claims abstract description 23
- 239000013307 optical fiber Substances 0.000 claims abstract description 8
- 238000000411 transmission spectrum Methods 0.000 claims abstract description 8
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/075—Investigating concentration of particle suspensions by optical means
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Abstract
The present invention is applied to optical fiber technology there is provided a kind of preparation method of hematocrite concentration sensor, including：The single-mode fiber for peeling coat off is placed on fiber clamp and adjusting is horizontally situated single-mode fiber；Femtosecond laser is focused on into the fibre core plane of single-mode fiber and energy is adjusted, according to default mach zhender chamber model, control femtosecond laser carries out by-line scanning machining to single-mode fiber and obtains sample fiber；Sample fiber is placed in etching solution and is corroded to prepare mach zhender chamber, sample fiber after cleaning corrosion is placed in the quality of micro- Microscopic observation corrosion mach zhender chamber and will test transmission spectrum, judge whether the quality and transmission spectrum of corroding mach zhender chamber reach pre-set criteria value, if not up to, then need Optimal Parameters, if reaching, the sample fiber after corrosion is regard as hematocrite concentration sensor.Sensor construction and manufacture craft that the present invention is provided are simple, reliability is high, sensitivity is high.
Description
Technical field
The invention belongs to technical field of optical fiber, more particularly to a kind of hematocrite concentration sensor and preparation method thereof, test
Device.
Background technology
Haemocyte detection is in the phenomenon to a certain degree with the health and disease that human body can be reflected in quantity.Normal adults
Haemocyte quantity and concentration be fixed within the specific limits, if more or less than range of normal value, can be corresponding
Show certain illness.
The spacecrafts such as Divine Land airship are although bulky, but inner space is limited, as cellanalyzer or fluidic cell
This kind of maxicell counting instruments such as instrument, due to it is bulky, professional operation is strong the features such as lead to not bring space into.And it is outer
Space environment is severe, strong electromagnetic, intense radiation cause usual device can not normal work, it is but how right in the presence of a harsh environment
The haemocyte of astronaut is detected, and then monitors the healthy situation of astronaut, is the weight for concerning astronaut's life security
Want problem.
The equipment volume of detection haemocyte is huge at present, and detection process is excessively complicated, and efficiency is low, and with certain pollution
Property.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of hematocrite concentration sensor and preparation method thereof, test
Device, it is intended to which the equipment volume for solving the detection haemocyte of prior art offer is huge, and detection process is excessively complicated, and efficiency is low
Problem.
The present invention is achieved in that a kind of preparation method of hematocrite concentration sensor, including：
The single-mode fiber for peeling coat off, which is placed on fiber clamp, and adjusts the fiber clamp makes the single-mode fiber
It is horizontally situated；
Femtosecond laser is focused on to the fibre core plane of the single-mode fiber, the energy of the femtosecond laser is adjusted, according to pre-
If Mach-Zehnder chamber model, control energy adjustment after femtosecond laser to the single-mode fiber carry out by-line scanning machining,
Obtain sample fiber；
The sample fiber is placed in preset etching solution and corroded, to etch Mach-Zehnder chamber；
Sample fiber after cleaning corrosion, is placed in micro- Microscopic observation by the sample fiber after corrosion and corrodes Mach-Zehnder
The quality of your chamber, the sample fiber after corrosion is accessed between light source and spectrometer, the transmission of the sample fiber after test corrosion
Spectrum；
Judge corrode Mach-Zehnder chamber quality or the corrosion after sample fiber transmission spectrum whether reach it is pre-
Standard value is put, if not up to, adjusting the machined parameters of the femtosecond laser or the parameter of etching, and new single-mode fiber is taken,
The step that the single-mode fiber for peeling coat off is placed on fiber clamp is performed, adjusting the machined parameters of the femtosecond laser includes
The energy of the femtosecond laser is adjusted, the parameter of adjustment etching includes adjusting concentration, the etching time of the etching solution；
If reaching, the sample fiber after corrosion is regard as hematocrite concentration sensor.
Further, it is described that the single-mode fiber for peeling coat off is placed on fiber clamp, and adjust the fiber clamp
Make the single-mode fiber be horizontally situated including：
The single-mode fiber for peeling coat off is fixed on the adjustable flexion-extension platform of two dimension, the flexion-extension platform is located at three-dimensional mobile
On platform；
The movement of the three-dimensional mobile platform is controlled, and adjusts the flexion-extension platform, makes the optical fiber axial direction of the single-mode fiber
It is parallel with the hot spot moving direction of the femtosecond laser.
Further, the energy of the regulation femtosecond laser includes：
The attenuator being made up of half-wave plate and Glan prism adjusts the energy of the femtosecond laser, by the femtosecond laser
Energy hole in 65nJ to 100nJ, modulated with the suitable refractive index intensity for forming local uniform.
Further, after the energy of the regulation femtosecond laser, in addition to：
The femtosecond laser is focused on to the covering and fibre of the fibre core plane of the single-mode fiber by 100 times of immersion oil object lens
Core intersection, and enter at about 3 μm to 5 μm of the fibre core.
Further, the step of configuring the etching solution includes：
Deionized water and alcohol is added to dilute for 40% hydrofluoric acid stoste mass fraction, using alcohol as cushioning liquid,
Configure the hydrofluoric acid solution that solution concentration is 5%-8%.
Further, the sample fiber is placed in into progress corrosion in preset hydrofluoric acid solution includes：
The sample fiber is placed in the hydrogen-oxygen acid solution, the temperature of corrosion is set as 40 DEG C to 45 DEG C, using water
Bathe mode of heating to be corroded, and add magnetic agitation and disturbed.
Further, etching time control was at 10 to 20 minutes.
Present invention also offers a kind of hematocrite concentration sensor, the hematocrite concentration sensor passes through described above
Preparation method is prepared.
Present invention also offers a kind of test device of hematocrite concentration, the test device is by dimethyl silicone polymer system
It is standby to obtain, including microchannel, the feed pathway that is connected with the microchannel and liquid outlet channel.
Further, a diameter of 300 μm to 359 μm of the microchannel.
Compared with prior art, beneficial effect is the present invention：The preparation method system provided using the embodiment of the present invention
Make the obtained hematocrite concentration sensor based on single-mode fiber, using all -fiber formula structure, electromagnetic interference can be avoided to detection
As a result influence.Meanwhile, the sensor construction and manufacture craft are simple, and reliability is high, in use, only need to be by the haemocyte solution
Using syringe from the feed pathway of test device injects the Mach-Zehnder chamber of single-mode fiber, the connection of single-mode fiber two ends
Light source and spectrometer are high with sensitivity detecting that hematocrite concentration changes by way of the drift situation for detecting interference spectrum
The characteristics of.
Brief description of the drawings
Fig. 1 is the structural representation of hematocrite concentration sensor provided in an embodiment of the present invention；
Fig. 2 is the flow chart of the preparation method of hematocrite concentration sensor provided in an embodiment of the present invention；
Fig. 3 is the structural representation of the preparation system of hematocrite concentration sensor provided in an embodiment of the present invention；
Fig. 4 is the scanning track schematic diagram of femtosecond laser provided in an embodiment of the present invention；
Fig. 5 is the model pictorial diagram of the Mach-Zehnder chamber of femtosecond laser processing and fabricating provided in an embodiment of the present invention；
Fig. 6 is the pictorial diagram of the Mach-Zehnder chamber provided in an embodiment of the present invention after hydrofluoric acid solution corrodes；
Fig. 7 is the aerial interference spectrogram of the single-mode fiber provided in an embodiment of the present invention for possessing Mach-Zehnder chamber；
Fig. 8 is the structural representation of the test device of hematocrite concentration provided in an embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
The embodiments of the invention provide a kind of femtosecond laser wet etching technique prepare hematocrite concentration sensor and its
Preparation method, by single-mode fiber internal production Mach-Zehnder MZ chambers, by detecting that the MZ intracavitary haemocytes solution is reflected
The detection to hematocrite concentration is realized in the change of rate.
Fig. 1 shows hematocrite concentration sensor provided in an embodiment of the present invention, to possess the single mode of Mach-Zehnder chamber
Optical fiber.Light is transmitted in the fibre core of single-mode fiber 101, is divided into two beams by MZ chambers 102, and light beam is passed still along fibre core
Defeated, light beam is transmitted by MZ chambers 102 in addition, and last two-beam is converged in the fibre core of single-mode fiber 101 again.Due to two beams
The refractive index for the medium that light passes through is different, produces optical path difference, so as to form MZ interference.Environmental liquids pass through microchannel 103
Change the refractive index of chamber medium into MZ chambers 102, so as to detect this change on transmitted spectrum.
Fig. 2 shows a kind of preparation method of hematocrite concentration sensor provided in an embodiment of the present invention, including：
S201, the single-mode fiber for peeling coat off is placed on fiber clamp, and is adjusted the fiber clamp and made the list
Mode fiber is horizontally situated；
S202, femtosecond laser is focused on the fibre core plane of the single-mode fiber, is adjusted the energy of the femtosecond laser, is pressed
According to default Mach-Zehnder chamber model, the femtosecond laser after control energy adjustment carries out by-line scanning to the single-mode fiber
Processing, obtains sample fiber；
S203, the sample fiber is placed in preset etching solution and corroded, to etch Mach-Zehnder chamber；
S204, the sample fiber after cleaning corrosion, by the sample fiber after corrosion be placed in micro- Microscopic observation corrode Mach-
The quality of Zeng Deer chambers, the sample fiber after corrosion is accessed between light source and spectrometer, the sample fiber after test corrosion
Transmission spectrum；
S205, judge corrode Mach-Zehnder chamber quality or the corrosion after sample fiber transmission spectrum whether
Pre-set criteria value is reached, if not up to, adjusting the machined parameters of the femtosecond laser or the parameter of etching, and take new single mode
Optical fiber, performs the step that the single-mode fiber for peeling coat off is placed on fiber clamp, adjusts the processing ginseng of the femtosecond laser
Number includes adjusting the energy of the femtosecond laser, and the parameter of adjustment etching includes adjusting concentration, the etching time of the etching solution.
In this step, if the quality for the Mach-Zehnder chamber that judgement corrosion is obtained or the transmission spectrum of sample fiber are not up to preset
Standard value, then this time the single-mode fiber of processing is undesirable, and the machined parameters of femtosecond laser or the parameter of etching need to be entered
Row adjustment, and takes new single-mode fiber, re-starts processing, the single-mode fiber discarding of original processing failure without.
S206, if reaching, regard the sample fiber after corrosion as hematocrite concentration sensor.
Below, specifically illustrated by providing the embodiment of the present invention preparation method of hematocrite concentration sensor：
Step 1, the single-mode fiber for peeling coat off is placed on fiber clamp, and adjusts the fiber clamp makes the list
Mode fiber is horizontally situated；
As shown in figure 3, the single-mode fiber for peeling coat off is fixed on the adjustable flexion-extension platform of two dimension, the flexion-extension platform is located at
In three-dimensional mobile platform.By adjusting two-dimentional adjustable pitching platform and three-dimensional mobile platform, make the fibre core axial direction of single-mode fiber
It is parallel with the hot spot moving direction of femtosecond laser.The attenuator of half-wave plate and Glan prism composition is adjusted, by the energy of femtosecond laser
Amount control enables femtosecond laser to form local more uniform suitable refractive index intensity modulation in 65-100nJ.By calculating
Machine control three-dimensional mobile platform movement, makes femtosecond laser focus at the fibre core and cladding interface of the fibre core plane of single-mode fiber,
Then moving Y-axis makes the focal spot of femtosecond laser enter about 3-5 μm of the fibre core of single-mode fiber.
Step 2, the femtosecond laser after control energy adjustment is scanned processing to the single-mode fiber, obtains optical fiber sample
Product.
During femtosecond laser scans track as shown in figure 4, specifically operating, femtosecond laser transfixion, moving three dimension movement
Platform, the movement of femtosecond laser is described by static object of reference of three-dimensional mobile platform below.Setting possesses high-precision
Three-dimensional mobile platform is 5-10 μm/s in the translational speed of X, Y, Z axis.Shutter is opened, three-dimensional mobile platform is controlled toward X-direction
Mobile, now femtosecond laser starts scanning, when femtosecond laser stops along X-axis after positive (or reverse) about 60-100 μm of scanning
Only, continue to move 2-3 μm along Z axis negative sense (forward direction), move and stop after 60-100 μm then along X-axis negative sense (forward direction), along Y-axis
Mobile 1-1.5 μm to negative direction (direction of vertical fibre core and remote fibre core), then femtosecond laser is positive (or reverse) along X-axis
Stop after about 60-100 μm of scanning, it is mobile 2-3 μm along Z axis positive (negative sense), move 60- then along X-axis negative sense (forward direction)
Stop after 100 μm, move 1-1.5 μm along Y-axis negative direction, above step is a cycle, so repeat three after multiple cycles
Completion of processing of the MZ chambers of dimension by way of by-line is scanned.Multiple microchannel connection MZ chambers and light are processed finally along Y-direction
Fine cladding surface, finally obtains the sample fiber with MZ chambers.Fig. 5 represents the MZ chambers after femtosecond laser parallel micromachining.Can be with by Fig. 5
Find out, it is processed after field color it is deeper because processing difference material be refracted rate modification, refractive index is than undressed region
Greatly, therefore micro- Microscopic observation color is deeper.
Step 3, it is configured to the hydrofluoric acid solution of corrosion.
Mass fraction is mixed with alcohol and deionized water respectively by volume for 40% hydrofluoric acid solution, volume is configured
Fraction is 5%-8% hydrofluoric acid solution.Plus the purpose of alcohol is the effect of buffering.The container material quality of hydrofluoric acid contact is poly- four
PVF.Sample fiber is cut off at the 2-3mm apart from MZ cavity configurations, is then inserted perpendicularly into and fills the hydrofluoric acid that configuration is completed
In the container of solution.It is 40-45 DEG C that magnetic stirring apparatus, which adds design temperature after water, has hydrofluoric acid solution after will be remained after temperature stabilization
Container be put into wherein, set etching time as 10-20 minutes, finally cleaned successively with ethanol and deionized water, be put into baking oven
Drying, by the sample fiber corroded and single-mode fiber welding.
Step 4, the MZ chamber quality sample fiber corroded being placed in after micro- Microscopic observation corrosion.
Situation about being likely to occur in practical operation has：Incomplete burn into chamber is in irregular shape, excessive corrosion causes fibre core
It is corroded completely.Such as there are the machined parameters that case above needs return to step 2 to change femtosecond laser, machined parameters include swashing
Light energy, sweep speed etc., or return to step 3 change the concentration and etching time of hydrofluoric acid solution, until corroding such as Fig. 5
Shown structure.Corrosion area material is completely removed as seen from Figure 6, and MZ chambers pass through microchannel UNICOM, fibre core with the external world
Also part retains.Sample fiber two ends after corrosion are connect into light source and spectrometer respectively, the interference spectrogram of MZ chambers is detected.If
It can't detect interference spectrum or spectral contrast be less than 5dB, then need return to step 2 or step 3 to change corresponding parameter, weight
Multiple experiment.Fig. 7 shows the interference spectrogram of a length of 98 μm of chamber, and interference contrast reaches 17dB.
Step 5, the test device of hematocrite concentration is made.
The solvent of PDMS (dimethyl silicone polymer) glue and curing agent are pressed 10:1 weight ratio is thoroughly mixed, then
It is placed in the bubble for vacuumizing and being extracted out in instrument in glue.A diameter of 300-350 μm of iron wire is placed in model, then will be exhausted
Glue after vacuum is poured into model, will be integrally positioned in sweat box and is toasted, and temperature setting is 80-90 DEG C, and the time is set as
1-1.5 hour.PDMS glue is fully cured after baking completely, extracts out after iron wire, microchannel is formed.Using card punch in microchannel
Two holes are made a call in top, are inserted into metal catheter formation feed pathway and liquid outlet channel.The test device completed such as Fig. 8 institutes
Show.
Step 6, the sample fiber etched is placed in the microchannel of test device, tests the concentration of haemocyte.
As shown in figure 8, the sample fiber etched is placed in the microchannel 807 of test device 800, microchannel 807
Two ends sealed with uv-curable glue.One termination Supercontinuum source 802 of sample fiber, another termination spectrometer 806 will be injected
Device 801 is connected with the feed pathway 803 of test device by plastic flexible pipe, and liquid outlet channel 804 connects with waste collecting device 805
Connect.Haemocyte solution is extracted with syringe 801, injection rate is set, haemocyte solution passes through feed pathway 803 and microchannel
807 enter in the MZ chambers of sample fiber, after record data after spectrum-stable.Then ethanol injection test device progress structure is changed clear
Wash, then change the haemocyte solution injection of another concentration, the spectrogram of the haemocyte of various concentrations is tested successively.Various concentrations
Haemocyte density it is different so as to showing otherness in terms of refractive index.When the haemocyte solution of various concentrations injects MZ
The refractive index of the medium of MZ chambers is changed after chamber, so as to cause the refringence between two interfere arms to change, is reflected in
It is exactly spectrally that wave length shift occurs for interference spectrum.By detecting that this wave length shift indirectly measures the change of hematocrite concentration
Change, and the hematocrite concentration sensor provided in an embodiment of the present invention for possessing MZ chambers is with very high refractive index sensitivity and very
Low refractive index detection limit, can detect 10-5Variations in refractive index, detect hematocrite concentration in terms of show excellent biography
Feel characteristic.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of preparation method of hematocrite concentration sensor, it is characterised in that including：
The single-mode fiber for peeling coat off is placed on fiber clamp, and adjusting the fiber clamp is located at the single-mode fiber
Horizontal level；
Femtosecond laser is focused on to the fibre core plane of the single-mode fiber, the energy of the femtosecond laser is adjusted, according to default
Femtosecond laser after Mach-Zehnder chamber model, control energy adjustment carries out by-line scanning machining to the single-mode fiber, obtains
Sample fiber；
The sample fiber is placed in preset etching solution and corroded, to etch Mach-Zehnder chamber；
Sample fiber after cleaning corrosion, is placed in micro- Microscopic observation by the sample fiber after corrosion and corrodes Mach-Zehnder chamber
Quality, the sample fiber after corrosion is accessed between light source and spectrometer, the transmission spectrum of the sample fiber after test corrosion；
Judge to corrode Mach-Zehnder chamber quality or the corrosion after the transmission spectrum of sample fiber whether reach preset mark
Quasi- value, if not up to, adjusting the machined parameters of the femtosecond laser or the parameter of etching, and takes new single-mode fiber, performs
The step that the single-mode fiber for peeling coat off is placed on fiber clamp, adjusting the machined parameters of the femtosecond laser includes adjustment
The energy of the femtosecond laser, the parameter of adjustment etching includes adjusting concentration, the etching time of the etching solution；
If reaching, the sample fiber after corrosion is regard as hematocrite concentration sensor.
2. preparation method as claimed in claim 1, it is characterised in that described that the single-mode fiber for peeling coat off is placed in optical fiber
On fixture, and adjust the fiber clamp make the single-mode fiber be horizontally situated including：
The single-mode fiber for peeling coat off is fixed on the adjustable flexion-extension platform of two dimension, the flexion-extension platform is located at three-dimensional mobile platform
On；
The movement of the three-dimensional mobile platform is controlled, and adjusts the flexion-extension platform, makes optical fiber axial direction and the institute of the single-mode fiber
The hot spot moving direction for stating femtosecond laser is parallel.
3. preparation method as claimed in claim 1, it is characterised in that the energy of the regulation femtosecond laser includes：
The attenuator being made up of half-wave plate and Glan prism adjusts the energy of the femtosecond laser, by the energy of the femtosecond laser
Amount control is modulated in 65nJ to 100nJ with the suitable refractive index intensity for forming local uniform.
4. preparation method as claimed in claim 1, it is characterised in that after the energy of the regulation femtosecond laser, also
Including：
The covering that the femtosecond laser is focused on to the fibre core plane of the single-mode fiber by 100 times of immersion oil object lens is handed over fibre core
At boundary, and enter at about 3 μm to 5 μm of the fibre core.
5. preparation method as claimed in claim 1, it is characterised in that the step of configuring the etching solution includes：
Add deionized water and alcohol to dilute for 40% hydrofluoric acid stoste mass fraction, regard alcohol as cushioning liquid, configuration
Solution concentration is 5%-8% hydrofluoric acid solution.
6. preparation method as claimed in claim 5, it is characterised in that the sample fiber is placed in preset hydrofluoric acid solution
Middle progress corrosion includes：
The sample fiber is placed in the hydrogen-oxygen acid solution, the temperature of corrosion is set as 40 DEG C to 45 DEG C, using water-bath plus
Hot mode is corroded, and is added magnetic agitation and disturbed.
7. preparation method as claimed in claim 1, it is characterised in that etching time was controlled at 10 to 20 minutes.
8. a kind of hematocrite concentration sensor, it is characterised in that the hematocrite concentration sensor passes through claim 1 to 7 times
Preparation method described in meaning one is prepared.
9. a kind of test device of hematocrite concentration, it is characterised in that the test device is prepared into by dimethyl silicone polymer
Arrive, including microchannel, the feed pathway that is connected with the microchannel and liquid outlet channel.
10. test device as claimed in claim 9, it is characterised in that a diameter of 300 μm to 359 μm of the microchannel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710695544.8A CN107314960B (en) | 2017-08-15 | 2017-08-15 | Blood cell concentration sensor, preparation method thereof and testing device |
Applications Claiming Priority (1)
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